Rmu_sc0000071.1_g000019

Golgi to ER traffic protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000071.1
OG0009398
Physical Location & Seq
Forward (+)
73372 .. 75844
2473 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 666 bp
atgtcgaaggagagagccaaaagaggcacactgcctccagcccaagagcaaattgagaaatttgagaaagttgtagaggaaggtaactactatggagctcagcagctgtacaaatctattagtgcgagatatgtggctgctcagaggtatactgaagctttagatattcttcagtcgggtgcatgcatccagttgaaacaagggcaggttacgtgtggggcagagttagctgctttgtttgtggatacacttgtcaaagcgagatgcccttatgacgatgaagccctcgttcgtgtcaggacaatttataaacagtttcctaagatacctgtgccacaacagttgggggatgatgatgacgtgcaacgactttctgaaacacttgggacagcaaaaacatgtgttgaaggatgctcatcattcctaaaagctgctatcaagtggtctgcggagtttggtgcacctaggggtggggctccagaaatacatgttatgctagctaactatatgtactttgaatcttctgaggtggacatggttagagttgcacagcattttgtcagaggaaacaacccaaaagaatttgcttctactttagtagatttcatgggcaagtgctatccaggggaagatgatgtggccattgcaagggcaattttaatgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF410

Protein Analysis

221

Amino Acids

24.53

Weight (kDa)

5.56

Isoelectric Point (pI)

38.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GET4 PF04190 48 - 221 1.8e-36 Golgi to ER traffic protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 309
Acc36I ACCTGC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 149
AciI CCGC 1 cut(s) 449
AcoI YGGCCR 1 cut(s) 639
AcsI RAATTY 2 cut(s) 59, 581
AcuI CTGAAG 2 cut(s) 155, 174
AfaI GTAC 2 cut(s) 110, 512
AfiI CCNNNNNNNGG 2 cut(s) 470, 648
AflIII ACRYGT 3 cut(s) 212, 398, 487
AgsI TTSAA 3 cut(s) 196, 407, 518
AjiI CACGTC 1 cut(s) 361
AjnI CCWGG 1 cut(s) 622
AluBI AGCT 6 cut(s) 98, 106, 158, 230, 431, 500
AluI AGCT 6 cut(s) 98, 106, 158, 230, 431, 500
Alw21I GWGCWC 2 cut(s) 100, 463
Alw44I GTGCAC 1 cut(s) 459
AlwNI CAGNNNCTG 1 cut(s) 106
AoxI GGCC 1 cut(s) 639
ApaLI GTGCAC 1 cut(s) 459
ApeKI GCWGC 4 cut(s) 103, 137, 230, 431
ApoI RAATTY 2 cut(s) 59, 581
AspA2I CCTAGG 1 cut(s) 464
AsuNHI GCTAGC 1 cut(s) 496
AvrII CCTAGG 1 cut(s) 464
BaeGI GKGCMC 1 cut(s) 463
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BalI TGGCCA 1 cut(s) 641
BanII GRGCYC 2 cut(s) 100, 478
Bbv12I GWGCWC 2 cut(s) 100, 463
BbvI GCAGC 4 cut(s) 115, 124, 217, 418
BciT130I CCWGG 1 cut(s) 624
BciVI GTATCC 1 cut(s) 238
BfaI CTAG 2 cut(s) 465, 497
BfuAI ACCTGC 1 cut(s) 196
BfuI GTATCC 1 cut(s) 238
BisI GCNGC 4 cut(s) 104, 138, 231, 432
BlnI CCTAGG 1 cut(s) 464
BlpI GCTNAGC 1 cut(s) 99
BlsI GCNGC 4 cut(s) 105, 139, 232, 433
Bme1390I CCNGG 1 cut(s) 624
BmgBI CACGTC 1 cut(s) 361
BmiI GGNNCC 1 cut(s) 477
BmrFI CCNGG 1 cut(s) 624
BmsI GCATC 3 cut(s) 195, 254, 401
BmtI GCTAGC 1 cut(s) 500
BpmI CTGGAG 2 cut(s) 21, 462
Bpu1102I GCTNAGC 1 cut(s) 99
BsaAI YACGTR 1 cut(s) 213
BsaBI GATNNNNATC 1 cut(s) 415
BsaJI CCNNGG 2 cut(s) 464, 623
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 2 cut(s) 470, 648
Bse1I ACTGG 1 cut(s) 190
Bse3DI GCAATG 1 cut(s) 642
Bse8I GATNNNNATC 1 cut(s) 415
BseBI CCWGG 1 cut(s) 624
BseDI CCNNGG 2 cut(s) 464, 623
BseGI GGATG 3 cut(s) 186, 355, 416
BseJI GATNNNNATC 1 cut(s) 415
BseLI CCNNNNNNNGG 2 cut(s) 470, 648
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 3 cut(s) 113, 155, 516
BseNI ACTGG 1 cut(s) 190
BseSI GKGCMC 1 cut(s) 463
BseXI GCAGC 4 cut(s) 115, 124, 217, 418
BshFI GGCC 1 cut(s) 641
BsiHKAI GWGCWC 2 cut(s) 100, 463
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 2 cut(s) 470, 648
BsmFI GGGAC 1 cut(s) 400
BsnI GGCC 1 cut(s) 641
Bsp1286I GDGCHC 3 cut(s) 100, 463, 478
Bsp1407I TGTACA 1 cut(s) 108
Bsp1720I GCTNAGC 1 cut(s) 99
BspACI CCGC 1 cut(s) 449
BspANI GGCC 1 cut(s) 641
BspCNI CTCAG 3 cut(s) 112, 154, 517
BspLI GGNNCC 1 cut(s) 477
BspMI ACCTGC 1 cut(s) 196
BspOI GCTAGC 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 642
BsrGI TGTACA 1 cut(s) 108
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 2 cut(s) 464, 623
BssNAI GTATAC 1 cut(s) 150
BssT1I CCWWGG 1 cut(s) 464
Bst1107I GTATAC 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 624
Bst4CI ACNGT 2 cut(s) 315, 342
BstAUI TGTACA 1 cut(s) 108
BstBAI YACGTR 1 cut(s) 213
BstC8I GCNNGC 2 cut(s) 184, 498
BstDEI CTNAG 4 cut(s) 99, 141, 321, 525
BstF5I GGATG 3 cut(s) 186, 355, 416
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstNI CCWGG 1 cut(s) 624
BstNSI RCATGY 3 cut(s) 186, 402, 491
BstSCI CCNGG 1 cut(s) 622
BstSLI GKGCMC 1 cut(s) 463
BstV1I GCAGC 4 cut(s) 115, 124, 217, 418
BstZ17I GTATAC 1 cut(s) 150
BsuI GTATCC 1 cut(s) 238
BsuRI GGCC 1 cut(s) 641
BtrI CACGTC 1 cut(s) 361
BtsCI GGATG 3 cut(s) 186, 355, 416
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 29
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 2 cut(s) 184, 498
CaiI CAGNNNCTG 1 cut(s) 106
Csp6I GTAC 2 cut(s) 109, 511
CspCI CAANNNNNGTGG 2 cut(s) 324, 359
CviAII CATG 5 cut(s) 183, 399, 488, 535, 607
CviQI GTAC 2 cut(s) 109, 511
DdeI CTNAG 4 cut(s) 99, 141, 321, 525
EaeI YGGCCR 1 cut(s) 639
Ecl136II GAGCTC 1 cut(s) 98
Eco130I CCWWGG 1 cut(s) 464
Eco24I GRGCYC 2 cut(s) 100, 478
Eco53kI GAGCTC 1 cut(s) 98
Eco57I CTGAAG 2 cut(s) 155, 174
EcoICRI GAGCTC 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 464
EcoT22I ATGCAT 1 cut(s) 188
EcoT38I GRGCYC 2 cut(s) 100, 478
ErhI CCWWGG 1 cut(s) 464
FaeI CATG 5 cut(s) 186, 402, 491, 538, 610
FaqI GGGAC 1 cut(s) 400
FatI CATG 5 cut(s) 182, 398, 487, 534, 606
FblI GTMKAC 1 cut(s) 149
Fnu4HI GCNGC 4 cut(s) 104, 138, 231, 432
FokI GGATG 3 cut(s) 173, 362, 423
FriOI GRGCYC 2 cut(s) 100, 478
Fsp4HI GCNGC 4 cut(s) 104, 138, 231, 432
FspBI CTAG 2 cut(s) 465, 497
GluI GCNGC 4 cut(s) 104, 138, 231, 432
GsuI CTGGAG 2 cut(s) 21, 462
HaeIII GGCC 1 cut(s) 641
Hin1II CATG 5 cut(s) 186, 402, 491, 538, 610
HindIII AAGCTT 1 cut(s) 156
HinfI GANTC 1 cut(s) 518
Hpy166II GTNNAC 3 cut(s) 150, 461, 532
Hpy188I TCNGA 4 cut(s) 144, 376, 526, 563
Hpy188III TCNNGA 2 cut(s) 298, 479
Hpy8I GTNNAC 3 cut(s) 150, 461, 532
HpyAV CCTTC 2 cut(s) 74, 401
HpyCH4III ACNGT 2 cut(s) 315, 342
HpyCH4IV ACGT 2 cut(s) 212, 360
HpyCH4V TGCA 6 cut(s) 182, 186, 364, 461, 548, 647
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 4 cut(s) 99, 141, 321, 525
HpySE526I ACGT 2 cut(s) 212, 360
Hsp92II CATG 5 cut(s) 186, 402, 491, 538, 610
LmnI GCTCC 2 cut(s) 95, 481
LpnPI CCDG 8 cut(s) 51, 191, 203, 283, 342, 492, 609, 636
Lsp1109I GCAGC 4 cut(s) 115, 124, 217, 418
LweI GCATC 3 cut(s) 195, 254, 401
MaeI CTAG 2 cut(s) 465, 497
MaeII ACGT 2 cut(s) 212, 360
MaeIII GTNAC 2 cut(s) 83, 208
MboII GAAGA 3 cut(s) 161, 513, 641
MhlI GDGCHC 3 cut(s) 100, 463, 478
MlsI TGGCCA 1 cut(s) 641
MluCI AATT 5 cut(s) 51, 59, 303, 581, 654
MluNI TGGCCA 1 cut(s) 641
MnlI CCTC 7 cut(s) 17, 45, 70, 138, 296, 520, 557
Mox20I TGGCCA 1 cut(s) 641
Mph1103I ATGCAT 1 cut(s) 188
MscI TGGCCA 1 cut(s) 641
MseI TTAA 1 cut(s) 659
Msp20I TGGCCA 1 cut(s) 641
MspA1I CMGCKG 1 cut(s) 106
MspR9I CCNGG 1 cut(s) 624
MvaI CCWGG 1 cut(s) 624
MwoI GCNNNNNNNGC 1 cut(s) 227
NheI GCTAGC 1 cut(s) 496
NlaIII CATG 5 cut(s) 186, 402, 491, 538, 610
NlaIV GGNNCC 1 cut(s) 477
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 3 cut(s) 186, 402, 491
PaeI GCATGC 1 cut(s) 186
PciI ACATGT 2 cut(s) 398, 487
PfeI GAWTC 1 cut(s) 518
PkrI GCNGC 4 cut(s) 105, 139, 232, 433
Ppu21I YACGTR 1 cut(s) 213
PscI ACATGT 2 cut(s) 398, 487
PsiI TTATAA 1 cut(s) 309
Psp124BI GAGCTC 1 cut(s) 100
Psp6I CCWGG 1 cut(s) 622
PspGI CCWGG 1 cut(s) 622
PspN4I GGNNCC 1 cut(s) 477
PstNI CAGNNNCTG 1 cut(s) 106
PvuII CAGCTG 1 cut(s) 106
RsaI GTAC 2 cut(s) 110, 512
RsaNI GTAC 2 cut(s) 109, 511
SacI GAGCTC 1 cut(s) 100
SaqAI TTAA 1 cut(s) 659
SatI GCNGC 4 cut(s) 104, 138, 231, 432
ScrFI CCNGG 1 cut(s) 624
SduI GDGCHC 3 cut(s) 100, 463, 478
SfaNI GCATC 3 cut(s) 195, 254, 401
SphI GCATGC 1 cut(s) 186
Sse9I AATT 5 cut(s) 51, 59, 303, 581, 654
SsiI CCGC 1 cut(s) 449
SspMI CTAG 2 cut(s) 465, 497
SstI GAGCTC 1 cut(s) 100
StyD4I CCNGG 1 cut(s) 622
StyI CCWWGG 1 cut(s) 464
TaaI ACNGT 2 cut(s) 315, 342
TaiI ACGT 2 cut(s) 215, 363
TaqI TCGA 1 cut(s) 5
TasI AATT 5 cut(s) 51, 59, 303, 581, 654
TatI WGTACW 2 cut(s) 108, 510
TfiI GAWTC 1 cut(s) 518
Tru1I TTAA 1 cut(s) 659
Tru9I TTAA 1 cut(s) 659
TscAI CASTG 1 cut(s) 36
TseI GCWGC 4 cut(s) 103, 137, 230, 431
TspDTI ATGAA 2 cut(s) 294, 595
TspRI CASTG 1 cut(s) 36
VneI GTGCAC 1 cut(s) 459
XapI RAATTY 2 cut(s) 59, 581
XceI RCATGY 3 cut(s) 186, 402, 491
XmaJI CCTAGG 1 cut(s) 464
XmiI GTMKAC 1 cut(s) 149
XspI CTAG 2 cut(s) 465, 497
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.