Rmu_sc0000079.1_g000002

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000079.1
OG0008935
Physical Location & Seq
Reverse (-)
5649 .. 7969
2321 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 594 bp
ctgccatctgatcatgctccagattcactgagcacagctgatcttcaacccttgatcctccactccacacacaaaatcgtccctcaaggcgggtatgaggatgatcaagaggaggaggagattccaaaaccacgaaggatcggccgatacgtcgccggaaaagtgaactttcgaccgggtcccgatcgcgtcgactgtagttgctccgatcttcattattcgtcaccggaggtggccggaagtgagagaaccagtcgggtcgggtcaccgggtttgggtcggagggttaacgtcgtctactataatgaagcaagcggcgggaggggtgttccactcaccgtcctcatggatttggagcctgggaccgtggacagcgtcagatctggacagatcttccgtcccgataactttgtcttcaatcaatctgatgctagaaacaactgggcgaaaggtcactacaccgaggtggctgatttgatcgattcagttctcgatgtgatgctaacagaggtggagaactgtgactgcttgcaagggttccaggtatgccattcttgggtggtggtgctagatctggaatgggaacacttatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

197

Amino Acids

21.86

Weight (kDa)

4.76

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 99 - 185 5.7e-16 Tubulin/FtsZ family, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 192, 297
AccII CGCG 1 cut(s) 189
AciI CCGC 3 cut(s) 90, 315, 318
AclWI GGATC 2 cut(s) 49, 146
AcoI YGGCCR 2 cut(s) 142, 234
AfiI CCNNNNNNNGG 3 cut(s) 89, 275, 556
AgsI TTSAA 2 cut(s) 47, 418
AjnI CCWGG 2 cut(s) 358, 540
AleI CACNNNNGTG 1 cut(s) 464
AluBI AGCT 1 cut(s) 38
AluI AGCT 1 cut(s) 38
Alw21I GWGCWC 1 cut(s) 35
AlwI GGATC 2 cut(s) 49, 146
AoxI GGCC 2 cut(s) 142, 234
AspS9I GGNCC 2 cut(s) 179, 363
AsuC2I CCSGG 2 cut(s) 177, 270
AsuHPI GGTGA 3 cut(s) 216, 258, 328
AvaII GGWCC 2 cut(s) 179, 363
BbsI GAAGAC 1 cut(s) 406
Bbv12I GWGCWC 1 cut(s) 35
BccI CCATC 1 cut(s) 13
BciT130I CCWGG 2 cut(s) 360, 542
BclI TGATCA 2 cut(s) 10, 103
BcnI CCSGG 2 cut(s) 177, 270
BfaI CTAG 2 cut(s) 432, 569
BfmI CTRYAG 1 cut(s) 196
BglII AGATCT 3 cut(s) 380, 390, 571
BisI GCNGC 1 cut(s) 316
BlsI GCNGC 1 cut(s) 317
Bme1390I CCNGG 4 cut(s) 177, 270, 360, 542
Bme18I GGWCC 2 cut(s) 179, 363
BmgT120I GGNCC 2 cut(s) 179, 363
BmiI GGNNCC 5 cut(s) 180, 181, 357, 364, 539
BmrFI CCNGG 4 cut(s) 177, 270, 360, 542
BmrI ACTGGG 1 cut(s) 451
BmsI GCATC 2 cut(s) 418, 489
BmuI ACTGGG 1 cut(s) 451
BpiI GAAGAC 1 cut(s) 406
BpuEI CTTGAG 1 cut(s) 69
BpuMI CCSGG 2 cut(s) 177, 270
Bsa29I ATCGAT 1 cut(s) 480
BsaJI CCNNGG 3 cut(s) 359, 366, 462
BsaWI WCCGGW 1 cut(s) 226
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 275, 556
Bse1I ACTGG 2 cut(s) 252, 446
BseBI CCWGG 2 cut(s) 360, 542
BseCI ATCGAT 1 cut(s) 480
BseDI CCNNGG 3 cut(s) 359, 366, 462
BseGI GGATG 1 cut(s) 106
BseLI CCNNNNNNNGG 3 cut(s) 89, 275, 556
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 2 cut(s) 252, 446
BseRI GAGGAG 3 cut(s) 125, 128, 131
BseX3I CGGCCG 1 cut(s) 142
Bsh1236I CGCG 1 cut(s) 189
Bsh1285I CGRYCG 3 cut(s) 145, 176, 187
BshFI GGCC 2 cut(s) 144, 236
BshVI ATCGAT 1 cut(s) 480
BsiEI CGRYCG 3 cut(s) 145, 176, 187
BsiHKAI GWGCWC 1 cut(s) 35
BsiSI CCGG 5 cut(s) 156, 176, 227, 237, 269
BslFI GGGAC 4 cut(s) 65, 165, 376, 384
BslI CCNNNNNNNGG 3 cut(s) 89, 275, 556
BsmFI GGGAC 4 cut(s) 65, 165, 376, 384
BsnI GGCC 2 cut(s) 144, 236
Bsp1286I GDGCHC 1 cut(s) 35
BspACI CCGC 3 cut(s) 90, 315, 318
BspANI GGCC 2 cut(s) 144, 236
BspCNI CTCAG 1 cut(s) 21
BspDI ATCGAT 1 cut(s) 480
BspFNI CGCG 1 cut(s) 189
BspLI GGNNCC 5 cut(s) 180, 181, 357, 364, 539
BspPI GGATC 2 cut(s) 49, 146
BsrI ACTGG 2 cut(s) 252, 446
BssECI CCNNGG 3 cut(s) 359, 366, 462
Bst2UI CCWGG 2 cut(s) 360, 542
Bst4CI ACNGT 4 cut(s) 197, 340, 367, 521
BstC8I GCNNGC 2 cut(s) 313, 530
BstDEI CTNAG 1 cut(s) 29
BstDSI CCRYGG 1 cut(s) 366
BstEII GGTNACC 1 cut(s) 264
BstF5I GGATG 1 cut(s) 106
BstFNI CGCG 1 cut(s) 189
BstMCI CGRYCG 3 cut(s) 145, 176, 187
BstNI CCWGG 2 cut(s) 360, 542
BstPI GGTNACC 1 cut(s) 264
BstSCI CCNGG 4 cut(s) 175, 268, 358, 540
BstSFI CTRYAG 1 cut(s) 196
BstUI CGCG 1 cut(s) 189
BstV2I GAAGAC 1 cut(s) 406
BstX2I RGATCY 3 cut(s) 380, 390, 571
BstYI RGATCY 3 cut(s) 380, 390, 571
BstZI CGGCCG 1 cut(s) 142
Bsu15I ATCGAT 1 cut(s) 480
BsuRI GGCC 2 cut(s) 144, 236
BsuTUI ATCGAT 1 cut(s) 480
BtgI CCRYGG 1 cut(s) 366
BtsCI GGATG 1 cut(s) 106
BtsIMutI CAGTG 1 cut(s) 26
Cac8I GCNNGC 2 cut(s) 313, 530
Cfr13I GGNCC 2 cut(s) 179, 363
ClaI ATCGAT 1 cut(s) 480
CseI GACGC 2 cut(s) 178, 364
CviAII CATG 2 cut(s) 14, 346
CviJI RGCY 5 cut(s) 38, 144, 236, 358, 470
CviKI_1 RGCY 5 cut(s) 38, 144, 236, 358, 470
DdeI CTNAG 1 cut(s) 29
EaeI YGGCCR 2 cut(s) 142, 234
EagI CGGCCG 1 cut(s) 142
EclXI CGGCCG 1 cut(s) 142
Eco47I GGWCC 2 cut(s) 179, 363
Eco52I CGGCCG 1 cut(s) 142
Eco91I GGTNACC 1 cut(s) 264
EcoO109I RGGNCCY 1 cut(s) 179
EcoO65I GGTNACC 1 cut(s) 264
EcoRII CCWGG 2 cut(s) 358, 540
FaeI CATG 2 cut(s) 17, 349
FaiI YATR 6 cut(s) 15, 96, 303, 347, 547, 592
FaqI GGGAC 4 cut(s) 65, 165, 376, 384
FatI CATG 2 cut(s) 13, 345
FauI CCCGC 2 cut(s) 83, 311
FbaI TGATCA 2 cut(s) 10, 103
FblI GTMKAC 2 cut(s) 192, 297
Fnu4HI GCNGC 1 cut(s) 316
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 1 cut(s) 316
FspBI CTAG 2 cut(s) 432, 569
GluI GCNGC 1 cut(s) 316
HaeIII GGCC 2 cut(s) 144, 236
HapII CCGG 5 cut(s) 156, 176, 227, 237, 269
HgaI GACGC 2 cut(s) 178, 364
Hin1II CATG 2 cut(s) 17, 349
HincII GTYRAC 2 cut(s) 193, 289
HindII GTYRAC 2 cut(s) 193, 289
HinfI GANTC 3 cut(s) 23, 121, 482
HpaI GTTAAC 1 cut(s) 289
HpaII CCGG 5 cut(s) 156, 176, 227, 237, 269
HphI GGTGA 3 cut(s) 216, 258, 328
Hpy166II GTNNAC 5 cut(s) 166, 193, 289, 298, 370
Hpy188I TCNGA 5 cut(s) 10, 208, 282, 380, 427
Hpy188III TCNNGA 7 cut(s) 20, 107, 182, 384, 401, 491, 575
Hpy8I GTNNAC 5 cut(s) 166, 193, 289, 298, 370
Hpy99I CGWCG 3 cut(s) 155, 194, 296
HpyAV CCTTC 1 cut(s) 129
HpyCH4III ACNGT 4 cut(s) 197, 340, 367, 521
HpyCH4IV ACGT 2 cut(s) 150, 291
HpyCH4V TGCA 1 cut(s) 532
HpyF3I CTNAG 1 cut(s) 29
HpySE526I ACGT 2 cut(s) 150, 291
Hsp92II CATG 2 cut(s) 17, 349
KflI GGGWCCC 1 cut(s) 179
Ksp22I TGATCA 2 cut(s) 10, 103
KspAI GTTAAC 1 cut(s) 289
LmnI GCTCC 3 cut(s) 22, 209, 355
LweI GCATC 2 cut(s) 418, 489
MaeI CTAG 2 cut(s) 432, 569
MaeII ACGT 2 cut(s) 150, 291
MaeIII GTNAC 4 cut(s) 222, 264, 452, 521
MboII GAAGA 4 cut(s) 35, 203, 385, 406
MflI RGATCY 3 cut(s) 380, 390, 571
MhlI GDGCHC 1 cut(s) 35
MmeI TCCRAC 1 cut(s) 260
MseI TTAA 1 cut(s) 288
MslI CAYNNNNRTG 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 38
MspI CCGG 5 cut(s) 156, 176, 227, 237, 269
MspR9I CCNGG 4 cut(s) 177, 270, 360, 542
MvaI CCWGG 2 cut(s) 360, 542
MvnI CGCG 1 cut(s) 189
NciI CCSGG 2 cut(s) 177, 270
NlaIII CATG 2 cut(s) 17, 349
NlaIV GGNNCC 5 cut(s) 180, 181, 357, 364, 539
NmuCI GTSAC 4 cut(s) 222, 264, 452, 521
OliI CACNNNNGTG 1 cut(s) 464
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PfeI GAWTC 3 cut(s) 23, 121, 482
PflFI GACNNNGTC 2 cut(s) 177, 374
PkrI GCNGC 1 cut(s) 317
Ple19I CGATCG 1 cut(s) 187
PpuMI RGGWCCY 1 cut(s) 179
Psp5II RGGWCCY 1 cut(s) 179
Psp6I CCWGG 2 cut(s) 358, 540
PspEI GGTNACC 1 cut(s) 264
PspGI CCWGG 2 cut(s) 358, 540
PspN4I GGNNCC 5 cut(s) 180, 181, 357, 364, 539
PspPI GGNCC 2 cut(s) 179, 363
PspPPI RGGWCCY 1 cut(s) 179
PsuI RGATCY 3 cut(s) 380, 390, 571
PsyI GACNNNGTC 2 cut(s) 177, 374
PvuI CGATCG 1 cut(s) 187
PvuII CAGCTG 1 cut(s) 38
RseI CAYNNNNRTG 1 cut(s) 464
SalI GTCGAC 1 cut(s) 191
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 1 cut(s) 316
Sau96I GGNCC 2 cut(s) 179, 363
ScrFI CCNGG 4 cut(s) 177, 270, 360, 542
SduI GDGCHC 1 cut(s) 35
SetI ASST 8 cut(s) 40, 153, 234, 294, 454, 468, 513, 546
SfaNI GCATC 2 cut(s) 418, 489
SfcI CTRYAG 1 cut(s) 196
SinI GGWCC 2 cut(s) 179, 363
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
SsiI CCGC 3 cut(s) 90, 315, 318
SspMI CTAG 2 cut(s) 432, 569
StyD4I CCNGG 4 cut(s) 175, 268, 358, 540
TaaI ACNGT 4 cut(s) 197, 340, 367, 521
TaiI ACGT 2 cut(s) 153, 294
TaqI TCGA 4 cut(s) 172, 192, 480, 492
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 3 cut(s) 23, 121, 482
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TscAI CASTG 1 cut(s) 33
TseFI GTSAC 4 cut(s) 222, 264, 452, 521
Tsp45I GTSAC 4 cut(s) 222, 264, 452, 521
TspDTI ATGAA 2 cut(s) 203, 321
TspGWI ACGGA 1 cut(s) 386
TspRI CASTG 1 cut(s) 33
Tth111I GACNNNGTC 2 cut(s) 177, 374
VpaK11BI GGWCC 2 cut(s) 179, 363
XmiI GTMKAC 2 cut(s) 192, 297
XspI CTAG 2 cut(s) 432, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.