Rorug05G0384400

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002401
Physical Location & Seq
Forward (+)
52166086 .. 52172533
6448 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0384400.1

Sequence Viewer

Length: 1257 bp
ATGAATTCGGGCGACCTGAACAAGGTGTGGGAGATCAGAGCGCTGAAAAGAAAGGATCGGCAGGAGGAAGCCGGCAAGATGCTAGACAAAATCGCCAAACAGGTCCAGCCCATTATGCGCAAACACAAATGGCGTGTCAAGCTTCTCTCTGAGATGTGCCCAACCAATCCTTCTCTTCTCGGGTTGAATGTGAACCGCGGTGTGCATGTGAAGCTGAGGCTGCGTAGGCCGAATAGGGATTTGGATTTCTATCCCTTTGATCAAGTTCTTGATACAATGCTTCACGAGCTTTGCCACAATGTTCACGGTCCTCACGATTCCAAATTCTACAAGCTATGGGATGAACTTAGGAAGGAATGCGAGGAGCTGATGACAAAGGGTGTAACTGGCACTGCGGAGGGGTTTGATCTTCCAGGGAGGCGTTTGGGAAGTGTTACACGTCAACCCCCTCTTTCATCTCTCCGCAAAACTGCACTTGCTGCTGCAGAAAAGAGAGCGGTTTTGGGATCTTTGATGCCATCTGGACCTAAGCGTCTTGGTGGTGATAGCTCTATGATGGCTGCACTTAGTCCGGTGCAAGCTGCTGCAATGGCTGCCGAGAGAAGATTGCAGGATGATATCTGGTGTGGTTCAGCATTTGCTGGAGCTTCTGAGGATGGAGAATGCAACTCTGGTACATCAGAGGACTGTGAAAACATAGGTGCAAGTGCAGGAAGTTCAAGGCTTCACAATGGTGCAAAAGTACAATCCTCAGATACATTACCACAAAAAAGAAGTCGCATACCTAATAAGAACACTTCTTCTCGATCATCCAAAGTCCATTTAGGATCCGAGTTTGTAGATCTATCCAACGACGACTCTGTATCTGGTTCTGACATGAGATCTCAGAAAAGAAGTCGTGAATCAGAAACCAGTTCATTCCCTCTATCCAATCGTCATCTAGAATCTAGTTTTGTGGATTTGTTGGGTATTTCAACCTCTGGGTCCGAACCTAATAATGATGGAACACATAATCTTGAAGAATCTGCTATGTGGCAGTGTGGAACATGCACTTTGTTAAATCCGCCATTAGCTCCAGTATGCGAGCTTTGTAGCACACAGAGACCAAAAGATGTTGGTACAAGTTACAAAATCTGGTCATGTAAATTCTGCACTTTGGAAAACAGTATGAAGTTGGAGAAATGCTCAGCATGCGGACAGTGGAGATACTCGCATGGTCCACCAATTGCAACCCAACCACCAAATCTGGGCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

418

Amino Acids

46.03

Weight (kDa)

8.69

Isoelectric Point (pI)

60.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WLM PF08325 8 - 204 2.6e-65 WLM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 531
Acc16I TGCGCA 1 cut(s) 119
AccBSI CCGCTC 1 cut(s) 497
AccII CGCG 1 cut(s) 198
AciI CCGC 7 cut(s) 196, 198, 395, 463, 497, 1064, 1194
AclWI GGATC 4 cut(s) 63, 514, 822, 835
AcsI RAATTY 3 cut(s) 4, 323, 1145
AfaI GTAC 3 cut(s) 676, 744, 1120
AfeI AGCGCT 1 cut(s) 42
AfiI CCNNNNNNNGG 2 cut(s) 22, 1247
AflIII ACRYGT 1 cut(s) 437
AgsI TTSAA 4 cut(s) 187, 720, 975, 1019
AjiI CACGTC 1 cut(s) 440
AjnI CCWGG 1 cut(s) 412
AjuI GAANNNNNNNTTGG 4 cut(s) 154, 186, 224, 256
AleI CACNNNNGTG 1 cut(s) 732
Alw26I GTCTC 1 cut(s) 1096
AlwI GGATC 4 cut(s) 63, 514, 822, 835
Ama87I CYCGRG 1 cut(s) 179
Aor51HI AGCGCT 1 cut(s) 42
AoxI GGCC 1 cut(s) 227
ApeKI GCWGC 7 cut(s) 220, 479, 482, 560, 581, 584, 593
ApoI RAATTY 3 cut(s) 4, 323, 1145
ArsI GACNNNNNNTTYG 4 cut(s) 120, 152, 760, 792
AspLEI GCGC 2 cut(s) 43, 120
AspS9I GGNCC 5 cut(s) 103, 308, 524, 984, 1217
AsuHPI GGTGA 1 cut(s) 554
AvaI CYCGRG 1 cut(s) 179
AvaII GGWCC 5 cut(s) 103, 308, 524, 984, 1217
BaeGI GKGCMC 2 cut(s) 161, 1253
BamHI GGATCC 1 cut(s) 827
BauI CACGAG 1 cut(s) 284
BbvCI CCTCAGC 1 cut(s) 215
BbvI GCAGC 7 cut(s) 207, 466, 469, 547, 568, 571, 580
BccI CCATC 4 cut(s) 526, 550, 650, 995
BciT130I CCWGG 1 cut(s) 414
BclI TGATCA 1 cut(s) 259
BcoDI GTCTC 1 cut(s) 1096
BfaI CTAG 3 cut(s) 83, 941, 948
BfmI CTRYAG 1 cut(s) 483
BfoI RGCGCY 1 cut(s) 44
BglII AGATCT 2 cut(s) 841, 881
BisI GCNGC 7 cut(s) 221, 480, 483, 561, 582, 585, 594
BlpI GCTNAGC 1 cut(s) 1186
BlsI GCNGC 7 cut(s) 222, 481, 484, 562, 583, 586, 595
Bme1390I CCNGG 1 cut(s) 414
Bme18I GGWCC 5 cut(s) 103, 308, 524, 984, 1217
BmeT110I CYCGRG 1 cut(s) 179
BmgBI CACGTC 1 cut(s) 440
BmgT120I GGNCC 5 cut(s) 103, 308, 524, 984, 1217
BmiI GGNNCC 2 cut(s) 829, 985
BmrFI CCNGG 1 cut(s) 414
BmsI GCATC 2 cut(s) 69, 504
BplI GAGNNNNNCTC 2 cut(s) 1169, 1201
BpmI CTGGAG 2 cut(s) 663, 1059
Bpu10I CCTNAGC 2 cut(s) 215, 528
Bpu1102I GCTNAGC 1 cut(s) 1186
BsaBI GATNNNNATC 1 cut(s) 249
BsaI GGTCTC 1 cut(s) 1096
BsaJI CCNNGG 2 cut(s) 196, 413
BsaWI WCCGGW 1 cut(s) 571
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 1247
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 3 cut(s) 391, 912, 1076
Bse3DI GCAATG 1 cut(s) 594
Bse8I GATNNNNATC 1 cut(s) 249
BseBI CCWGG 1 cut(s) 414
BseDI CCNNGG 2 cut(s) 196, 413
BseGI GGATG 4 cut(s) 346, 619, 661, 809
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 2 cut(s) 22, 1247
BseMI GCAATG 1 cut(s) 594
BseMII CTCAG 6 cut(s) 141, 206, 642, 765, 899, 1200
BseNI ACTGG 3 cut(s) 391, 912, 1076
BseRI GAGGAG 1 cut(s) 377
BseSI GKGCMC 2 cut(s) 161, 1253
BseXI GCAGC 7 cut(s) 207, 466, 469, 547, 568, 571, 580
BsgI GTGCAG 4 cut(s) 456, 546, 729, 1135
Bsh1236I CGCG 1 cut(s) 198
BshFI GGCC 1 cut(s) 229
BsiHKCI CYCGRG 1 cut(s) 179
BsiSI CCGG 2 cut(s) 72, 572
BslI CCNNNNNNNGG 2 cut(s) 22, 1247
BsmAI GTCTC 1 cut(s) 1096
BsmI GAATGC 2 cut(s) 362, 668
BsnI GGCC 1 cut(s) 229
Bso31I GGTCTC 1 cut(s) 1096
BsoBI CYCGRG 1 cut(s) 179
Bsp1286I GDGCHC 2 cut(s) 161, 1253
Bsp143I GATC 9 cut(s) 33, 55, 259, 406, 506, 806, 827, 841, 881
Bsp1720I GCTNAGC 1 cut(s) 1186
BspACI CCGC 7 cut(s) 196, 198, 395, 463, 497, 1064, 1194
BspANI GGCC 1 cut(s) 229
BspCNI CTCAG 6 cut(s) 142, 207, 643, 764, 898, 1199
BspFNI CGCG 1 cut(s) 198
BspLI GGNNCC 2 cut(s) 829, 985
BspMAI CTGCAG 1 cut(s) 487
BspPI GGATC 4 cut(s) 63, 514, 822, 835
BspTNI GGTCTC 1 cut(s) 1096
BsrBI CCGCTC 1 cut(s) 497
BsrDI GCAATG 1 cut(s) 594
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 3 cut(s) 391, 912, 1076
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 2 cut(s) 196, 413
BssMI GATC 9 cut(s) 33, 55, 259, 406, 506, 806, 827, 841, 881
BssSI CACGAG 1 cut(s) 284
Bst2BI CACGAG 1 cut(s) 284
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 4 cut(s) 308, 689, 1166, 1200
Bst6I CTCTTC 1 cut(s) 180
BstAPI GCANNNNNTGC 2 cut(s) 479, 593
BstC8I GCNNGC 4 cut(s) 73, 579, 1085, 1192
BstDEI CTNAG 9 cut(s) 150, 215, 347, 528, 566, 651, 751, 885, 1186
BstDSI CCRYGG 1 cut(s) 196
BstENI CCTNNNNNAGG 1 cut(s) 20
BstF5I GGATG 4 cut(s) 346, 619, 661, 809
BstFNI CGCG 1 cut(s) 198
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 2 cut(s) 43, 120
BstKTI GATC 9 cut(s) 36, 58, 262, 409, 509, 809, 830, 844, 884
BstMAI GTCTC 1 cut(s) 1096
BstMBI GATC 9 cut(s) 33, 55, 259, 406, 506, 806, 827, 841, 881
BstNI CCWGG 1 cut(s) 414
BstNSI RCATGY 3 cut(s) 209, 1050, 1194
BstSCI CCNGG 1 cut(s) 412
BstSFI CTRYAG 1 cut(s) 483
BstSLI GKGCMC 2 cut(s) 161, 1253
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 7 cut(s) 207, 466, 469, 547, 568, 571, 580
BstX2I RGATCY 4 cut(s) 506, 827, 841, 881
BstYI RGATCY 4 cut(s) 506, 827, 841, 881
BsuRI GGCC 1 cut(s) 229
BtgI CCRYGG 1 cut(s) 196
BtrI CACGTC 1 cut(s) 440
BtsCI GGATG 4 cut(s) 346, 619, 661, 809
BtsI GCAGTG 2 cut(s) 390, 1043
BtsIMutI CAGTG 3 cut(s) 390, 1043, 1205
Cac8I GCNNGC 4 cut(s) 73, 579, 1085, 1192
CfoI GCGC 2 cut(s) 43, 120
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 5 cut(s) 103, 308, 524, 984, 1217
Cfr42I CCGCGG 1 cut(s) 199
CseI GACGC 1 cut(s) 521
Csp6I GTAC 3 cut(s) 675, 743, 1119
CviAII CATG 6 cut(s) 206, 877, 1047, 1140, 1191, 1214
CviQI GTAC 3 cut(s) 675, 743, 1119
DdeI CTNAG 9 cut(s) 150, 215, 347, 528, 566, 651, 751, 885, 1186
DpnI GATC 9 cut(s) 35, 57, 261, 408, 508, 808, 829, 843, 883
DpnII GATC 9 cut(s) 33, 55, 259, 406, 506, 806, 827, 841, 881
DrdI GACNNNNNNGTC 1 cut(s) 531
DseDI GACNNNNNNGTC 1 cut(s) 531
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
EciI GGCGGA 1 cut(s) 1053
Eco31I GGTCTC 1 cut(s) 1096
Eco32I GATATC 1 cut(s) 619
Eco47I GGWCC 5 cut(s) 103, 308, 524, 984, 1217
Eco47III AGCGCT 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 179
EcoNI CCTNNNNNAGG 1 cut(s) 20
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 412
EcoRV GATATC 1 cut(s) 619
FaeI CATG 6 cut(s) 209, 880, 1050, 1143, 1194, 1217
FatI CATG 6 cut(s) 205, 876, 1046, 1139, 1190, 1213
FbaI TGATCA 1 cut(s) 259
Fnu4HI GCNGC 7 cut(s) 221, 480, 483, 561, 582, 585, 594
FokI GGATG 4 cut(s) 353, 626, 668, 796
Fsp4HI GCNGC 7 cut(s) 221, 480, 483, 561, 582, 585, 594
FspBI CTAG 3 cut(s) 83, 941, 948
FspI TGCGCA 1 cut(s) 119
GlaI GCGC 2 cut(s) 42, 119
GluI GCNGC 7 cut(s) 221, 480, 483, 561, 582, 585, 594
GsuI CTGGAG 2 cut(s) 663, 1059
HaeII RGCGCY 1 cut(s) 44
HaeIII GGCC 1 cut(s) 229
HapII CCGG 2 cut(s) 72, 572
HgaI GACGC 1 cut(s) 521
HhaI GCGC 2 cut(s) 43, 120
Hin1II CATG 6 cut(s) 209, 880, 1050, 1143, 1194, 1217
Hin6I GCGC 2 cut(s) 41, 118
HinP1I GCGC 2 cut(s) 41, 118
HincII GTYRAC 1 cut(s) 443
HindII GTYRAC 1 cut(s) 443
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 5 cut(s) 317, 857, 902, 944, 1022
HpaII CCGG 2 cut(s) 72, 572
HphI GGTGA 1 cut(s) 554
Hpy166II GTNNAC 4 cut(s) 193, 304, 443, 1220
Hpy188III TCNNGA 8 cut(s) 269, 284, 314, 522, 804, 899, 941, 1016
Hpy8I GTNNAC 4 cut(s) 193, 304, 443, 1220
Hpy99I CGWCG 1 cut(s) 857
HpyAV CCTTC 2 cut(s) 180, 346
HpyCH4III ACNGT 4 cut(s) 308, 689, 1166, 1200
HpyCH4IV ACGT 1 cut(s) 439
HpyF3I CTNAG 9 cut(s) 150, 215, 347, 528, 566, 651, 751, 885, 1186
HpySE526I ACGT 1 cut(s) 439
Hsp92II CATG 6 cut(s) 209, 880, 1050, 1143, 1194, 1217
HspAI GCGC 2 cut(s) 41, 118
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
Ksp22I TGATCA 1 cut(s) 259
KspI CCGCGG 1 cut(s) 199
Kzo9I GATC 9 cut(s) 33, 55, 259, 406, 506, 806, 827, 841, 881
LmnI GCTCC 3 cut(s) 364, 644, 1078
Lsp1109I GCAGC 7 cut(s) 207, 466, 469, 547, 568, 571, 580
LweI GCATC 2 cut(s) 69, 504
MaeI CTAG 3 cut(s) 83, 941, 948
MaeII ACGT 1 cut(s) 439
MaeIII GTNAC 3 cut(s) 382, 433, 1124
MalI GATC 9 cut(s) 35, 57, 261, 408, 508, 808, 829, 843, 883
MbiI CCGCTC 1 cut(s) 497
MboI GATC 9 cut(s) 33, 55, 259, 406, 506, 806, 827, 841, 881
MboII GAAGA 5 cut(s) 167, 401, 615, 792, 1031
MfeI CAATTG 1 cut(s) 1224
MflI RGATCY 4 cut(s) 506, 827, 841, 881
MhlI GDGCHC 2 cut(s) 161, 1253
MluCI AATT 4 cut(s) 4, 323, 1145, 1224
MlyI GAGTC 1 cut(s) 851
MmeI TCCRAC 2 cut(s) 873, 1155
MroNI GCCGGC 1 cut(s) 71
MseI TTAA 1 cut(s) 1058
MslI CAYNNNNRTG 1 cut(s) 732
MspA1I CMGCKG 1 cut(s) 198
MspI CCGG 2 cut(s) 72, 572
MspR9I CCNGG 1 cut(s) 414
MunI CAATTG 1 cut(s) 1224
Mva1269I GAATGC 2 cut(s) 362, 668
MvaI CCWGG 1 cut(s) 414
MvnI CGCG 1 cut(s) 198
NaeI GCCGGC 1 cut(s) 73
NdeII GATC 9 cut(s) 33, 55, 259, 406, 506, 806, 827, 841, 881
NgoMIV GCCGGC 1 cut(s) 71
NlaIII CATG 6 cut(s) 209, 880, 1050, 1143, 1194, 1217
NlaIV GGNNCC 2 cut(s) 829, 985
NmeAIII GCCGAG 1 cut(s) 622
NsbI TGCGCA 1 cut(s) 119
NspI RCATGY 3 cut(s) 209, 1050, 1194
OliI CACNNNNGTG 1 cut(s) 732
PaeI GCATGC 1 cut(s) 1194
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PctI GAATGC 2 cut(s) 362, 668
PdiI GCCGGC 1 cut(s) 73
PfeI GAWTC 4 cut(s) 317, 902, 944, 1022
PkrI GCNGC 7 cut(s) 222, 481, 484, 562, 583, 586, 595
PleI GAGTC 1 cut(s) 851
PpsI GAGTC 1 cut(s) 851
Psp6I CCWGG 1 cut(s) 412
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 2 cut(s) 829, 985
PspPI GGNCC 5 cut(s) 103, 308, 524, 984, 1217
PstI CTGCAG 1 cut(s) 487
PsuI RGATCY 4 cut(s) 506, 827, 841, 881
RsaI GTAC 3 cut(s) 676, 744, 1120
RsaNI GTAC 3 cut(s) 675, 743, 1119
RseI CAYNNNNRTG 1 cut(s) 732
SacII CCGCGG 1 cut(s) 199
SaqAI TTAA 1 cut(s) 1058
SatI GCNGC 7 cut(s) 221, 480, 483, 561, 582, 585, 594
Sau3AI GATC 9 cut(s) 33, 55, 259, 406, 506, 806, 827, 841, 881
Sau96I GGNCC 5 cut(s) 103, 308, 524, 984, 1217
SchI GAGTC 1 cut(s) 851
ScrFI CCNGG 1 cut(s) 414
SduI GDGCHC 2 cut(s) 161, 1253
SfaNI GCATC 2 cut(s) 69, 504
SfcI CTRYAG 1 cut(s) 483
Sfr303I CCGCGG 1 cut(s) 199
SgrBI CCGCGG 1 cut(s) 199
SinI GGWCC 5 cut(s) 103, 308, 524, 984, 1217
SmiMI CAYNNNNRTG 1 cut(s) 732
SphI GCATGC 1 cut(s) 1194
Sse9I AATT 4 cut(s) 4, 323, 1145, 1224
SsiI CCGC 7 cut(s) 196, 198, 395, 463, 497, 1064, 1194
SspMI CTAG 3 cut(s) 83, 941, 948
StyD4I CCNGG 1 cut(s) 412
TaaI ACNGT 4 cut(s) 308, 689, 1166, 1200
TaiI ACGT 1 cut(s) 442
TaqI TCGA 1 cut(s) 805
TasI AATT 4 cut(s) 4, 323, 1145, 1224
TatI WGTACW 1 cut(s) 742
TfiI GAWTC 4 cut(s) 317, 902, 944, 1022
Tru1I TTAA 1 cut(s) 1058
Tru9I TTAA 1 cut(s) 1058
TscAI CASTG 3 cut(s) 397, 1043, 1205
TseI GCWGC 7 cut(s) 220, 479, 482, 560, 581, 584, 593
TspDTI ATGAA 5 cut(s) 17, 357, 444, 906, 1184
TspRI CASTG 3 cut(s) 397, 1043, 1205
VpaK11BI GGWCC 5 cut(s) 103, 308, 524, 984, 1217
XagI CCTNNNNNAGG 1 cut(s) 20
XapI RAATTY 3 cut(s) 4, 323, 1145
XbaI TCTAGA 1 cut(s) 940
XceI RCATGY 3 cut(s) 209, 1050, 1194
XspI CTAG 3 cut(s) 83, 941, 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.