Rh5BG461100

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002401
Physical Location & Seq
Reverse (-)
73660636 .. 73665015
4380 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG461100.1

Sequence Viewer

Length: 3948 bp
ATGGGATTTGATAATGAGTGCATACTGAACATCCAATCTCTTGCGGGAGAGTACTTTTGCCCGGTCTGTCGTCTTCTTGTCTACCCAAATGAAGCATTGCAGTCACAGTGCACTCATCTATACTGTAAGCCCTGTTTGACATACGTCGTGAGCACTACCAAAGCGTGTCCATACGATGGCTACTTAGTGACAGAAGCCGATGCGAAGCCCCTCGTTGAATCAAATAAAGTGCTCGCTGAAACCATAGGGAAAATAGCAGTTCATTGCCTGTATCACAGGAGTGGGTGCACATGGCAGGGGCCTTTATCTGATTGCACATCTCATTGTTCTGGTTGTGCCTTTGGAAATTCTCCTGTAGTCTGCAACAGGTGTGGAATCCAAATTGTGCATCGTCAAGTGCAGGAACACGCACAAAGTTGCCCTGGTGTGCAGCCCCAAGCACAGCAGGCAGAGGGTGTTCCAGACACTTCAGCCTCTGGCACAGCTGCTACTGCCAATCAAAACCAGGCTGCCACTCAGACAGTTACAGTGACGTCTCAGACACAGGCTCCCCAAACTGCAAGTGCCACTATACCTGGAGCTGATTCGAATTCTCAGGCTCAAGCTGTTGTTCAAGCTGCTGCTCCGACTGCTGACCAGTGGTATCAGCAACAATATCAGCAGTACTACCAGCAGTATCCTGGATATGATCCATACCAACAACAGTATCAGCAGTACTATCCCTATCAGCAGCCAGCAATTCCACAACCACAGCAGCCTTTGCAGGCTAATCCACCCTATGGGACTGTTCAGCCTCAACCGCAGGCATACCTGCAACCTCCGCTCCTACCTCAACCTCAACCTCAACCTCAACCTCAACCTCAACCACAGACCCAACCATCTCAATCTCAAGCTCAACCCCAACCACAAGTTCAGCCCCCAATTATGCAGGCTCCTATGGCTGCGCATACTCAAAACCAGACATCAGTCAATCAACAGCTTCAACTTCAGCCTGCTATGGCACCACATTCACAAATTCAGGCACAGTCTTACCCATCAGCTCATGGGCAGGCTCAACTTCCTCCTCAAGCATATTTCCAAGTCCAAATGCCTCAATATCAGCAGCCACAAGTTCAGCAGCTTACACAGTCCCAGCTTCAACAGAATCCCCAACTTCACCCGTCTCAGCCCATGAATGCTACAGTCCAGTCCCAGACACAGCTCCCATCATCTAATTCATCGACTGGCAATCATTTGTTCCCTCAATCTCACCTTCCTCAACCAGTGCTCTCAGCAGCCTCCCAACAACGTACTATGCAGGTGCAATCTCAAGGAGTGCCCAACTCTCAGTCGCAAAACCATGTTCAGACTCAGATTCAGTTTCCTCAGCAACCCCTTTTGCGACCACCCCCGTTTCAAACAATAGTTCCAAACCAGCAGCAGACAGCCTTGCTGCCTTCACCTAACGTTATCTCTGCACAACAGCCTCCAGTTCACTCCTTTGCTCAACAATCTGGAATTCCTGTTCTCCAGCGCCCTGCAATGCAGCCAGTCCAACAGCTAAATCCTCAGCAATATTTCCACATACAACAGACACCCCCCACATTGTCGCAATTACGTCCCCAGGGCCAATCTCAATCATTCCCACAACATATTCGTGCCTCGAACCAGTCTCAGCAGAATGTTGTATTGTCTCAGGGCATGCAACATATTCAGCCCTCAAATCTGGGACGGCCTATGATGCCAAGTCATGGAGTACTACCTCAGCCATATGCACAAACAGTAGGAGGAGTTCTGCCAAGACCAATGTATCCTGCTCTAAACCATCAATCTACAAATCAGAATAATATAGGCAGAACCAACAACCAAGTGCAACTGGGAGCAAATTCAAGACCAACAATGACCGCAAGGCCGGCAGAGAAAGAAGCTGAGTTACCTGCTAATAATGTTGCACAGAATTTGGGTGTATTTTCTGCTGTAGGTGCTGATTCCGAGGTTAAAACTGTGAAATCTGAGGTGGATATGAAGTCCATTGATGTTGGAAATAAACCTTCTTCTGAAGATAGAAGTTATCAGGGTAATACATCTGCCAAAGAGATTCCGGAGTCTAGGGGGATGTTGGGGGCGAATGGAGAGTCTGAAAGTAAGCCAACATTGAAGGAAGAGGGTGTAAATAGTACTCTGGAGGATTCAAGTAATGATAAATTGGGTGAACTTGTAGCTGAAGGTGCAAAAGATGTTCCCAGCAGTGGCATGAAACAGGGGGAGCGTAAAGAAATGCCATCAGAAGAAGCACAACTTCATGGAGTAAAGGATCAAAAATTGCAAAAAGATGCTAGTGGCACCGAGGACGGTTCCCAAACTGTTTCTACTTCTTCAGCTCCTATAGGTCAAGTACAAGCTGGCGGCTTGATGCAACCTTCATATCCTGGCTCTGCTATACTCCAGCAAAGGCCAGGTGCACCTCCTTCATTACAAGTGCCTTCTTCAGGGCCTCAACATCATATACCGGGTTCTGGACAACCCTTAATTCATGCAAGGCCTCAGGGACCTGGACATGTGCCTGGGCAGGCTTCTCATCTATCTGAGCATTTTCAATCACCTAGGGGAAACCTGGGATTTGCAGCATCATCTGCAAGTGCAAGTCAGCATGGGCCTTATAACCAAAGCCATGCGCCTCCTCATCCAGGGGCACCCAGAGGTCCACCATTTGGAGCACCCCCTAGTGCTTTTGATTTTCATGGGGGTATAATGGCCAGAGCAGCACCCCATGGCCATGAAGGTCAGAGGGGTCAGCAGCGGCCGGCTTTTCAGATGGAACAGGGAGCAACTGGACAGCCATCCTCAAACATGCTCAGAATGAATGGAAATCCAGGCTTCGAATCCTTATCAACTCCCGGGTTGAGGGATGAAAGATTTAAGGCTTTGCCGGATGGGAGGTTGAATCCCTTCCCAGGAGACCCAACTCGTGTGATTAATCGATTAGGGTTTGAAGATGATCTCAAGCAATTCCCTAGGCCTTCTTATCTGGATTCTGAGCCTCTTCCAAAATTAGGGAGTTACTCCTCGAGAGCCTTCGATAGGAGACCTCTTGGAGTCAATTATGATACTCGTCTAAATTCAGATCCTGCAGCTGGCTCTGCTCCAAGGTTCCTGTCTCCCTATGGTCATGGTGGATTAATTCATGCCAATGATACAGGTGGTCACCCAGATTTTGGTGGACAGCGCCTTATGGATGGTTTGGCTCGACGAAGTCCAATCAGAGATTACCCTGGCATCCCATCTCGTGGATTTAGGGGATTTGGCCCTGATGATTTTGATGGCAGGGATTTCCATCGATTTGGTGATCTGCTTGGCAGGGAATTCCATGAGAATAGGTTTCCTAATATGCAGAGTCATTTTCATAGGGGTGAGTTTGAGGGTCCTGGTAATATGCGAATGGATGATCATATGAGGAACGATTTGATTGGTCAAGGTGGTCACCCTGGTCATCTGCGGAGGGGAGATCATTTGGGTCCTCATAACTTGCCTGGTCATTTGCATTTGCGAGAGCATGTGGGTTTTGGTGTCCATCCTCGACATGCAGGGCCTGGGAGCTTTGAATCATTTATTGGCAACAGGGCAAGTCATCCACGTCTTGGCGAGCCTGGATTTAGGAGCAGCTTTTCACTTCAGAGATTTCCAAATGATGGGACTTATACAGGAGAGCTAGAGTCTTTTGATCACTCAAGGAAAAGGAAACCAGCCAGCATGGGTTGGTGTCGTATCTGTAAAGTTGACTGTGAAACAGTTGAGGGCTTGGACGTGCATTCACAAACAAGGGAGCACCAGAGAATGGCTATGGAAATGGTACAAAGTATCAAGCAGAATGCCAAAAAGCAGAAATTAACCTCTGGTGATCAGTCCTCGGTAGAGGATGCAAACAAGTCAAAGATTTCCAGTTTTGAGAGCCGGGTTGAAAAGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000793 GO:0000794 GO:0001701 GO:0001756 GO:0001932 GO:0002020 GO:0002791 GO:0002793 GO:0003002 GO:0003007 GO:0003008 GO:0003012 GO:0003013 GO:0003015 GO:0003300 GO:0003674 GO:0003779 GO:0003824 GO:0004672 GO:0004674 GO:0004713 GO:0005198 GO:0005200 GO:0005488 GO:0005509 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005856 GO:0005859 GO:0005865 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006936 GO:0006941 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0007399 GO:0007417 GO:0007420 GO:0007507 GO:0007512 GO:0007517 GO:0008015 GO:0008092 GO:0008150 GO:0008152 GO:0008307 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009653 GO:0009790 GO:0009792 GO:0009887 GO:0009888 GO:0009893 GO:0009952 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010604 GO:0010628 GO:0010737 GO:0010927 GO:0014706 GO:0014866 GO:0014896 GO:0014897 GO:0015629 GO:0016043 GO:0016301 GO:0016310 GO:0016459 GO:0016460 GO:0016740 GO:0016772 GO:0016773 GO:0018108 GO:0018193 GO:0018212 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0021591 GO:0022607 GO:0023052 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030036 GO:0030154 GO:0030239 GO:0030240 GO:0030241 GO:0030506 GO:0031032 GO:0031033 GO:0031034 GO:0031323 GO:0031399 GO:0031430 GO:0031433 GO:0031672 GO:0031674 GO:0031974 GO:0031981 GO:0032268 GO:0032501 GO:0032502 GO:0032879 GO:0032880 GO:0032989 GO:0032991 GO:0033058 GO:0034622 GO:0035051 GO:0035265 GO:0035282 GO:0035556 GO:0035994 GO:0035995 GO:0036211 GO:0036379 GO:0040007 GO:0040011 GO:0042221 GO:0042325 GO:0042692 GO:0042802 GO:0042805 GO:0043009 GO:0043056 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043292 GO:0043412 GO:0043549 GO:0043621 GO:0043933 GO:0044057 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044449 GO:0044464 GO:0044877 GO:0045214 GO:0045859 GO:0046872 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048589 GO:0048644 GO:0048646 GO:0048729 GO:0048731 GO:0048738 GO:0048739 GO:0048747 GO:0048769 GO:0048856 GO:0048869 GO:0050708 GO:0050714 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0050982 GO:0051015 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051146 GO:0051171 GO:0051174 GO:0051222 GO:0051223 GO:0051239 GO:0051246 GO:0051338 GO:0051371 GO:0051393 GO:0051592 GO:0051606 GO:0051716 GO:0055001 GO:0055002 GO:0055003 GO:0055006 GO:0055007 GO:0055008 GO:0055013 GO:0060047 GO:0060048 GO:0060255 GO:0060322 GO:0060415 GO:0060419 GO:0060429 GO:0060537 GO:0061053 GO:0061061 GO:0065003 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070925 GO:0071688 GO:0071704 GO:0071840 GO:0072359 GO:0080090 GO:0090087 GO:0090257 GO:0097435 GO:0097493 GO:0099080 GO:0099081 GO:0099512 GO:0140096 GO:1901077 GO:1901564 GO:1901897 GO:1903530 GO:1903532 GO:1904951
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1315

Amino Acids

143.34

Weight (kDa)

7.22

Isoelectric Point (pI)

61.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2640
AarI CACCTGC 1 cut(s) 1288
AatII GACGTC 1 cut(s) 536
Acc16I TGCGCA 1 cut(s) 945
Acc36I ACCTGC 3 cut(s) 819, 1288, 1924
AccB1I GGYRCC 3 cut(s) 1000, 2321, 2671
AccB7I CCANNNNNTGG 9 cut(s) 176, 779, 1730, 2690, 3194, 3266, 3617, 3668, 3814
AccBSI CCGCTC 1 cut(s) 823
AccI GTMKAC 1 cut(s) 81
AccIII TCCGGA 1 cut(s) 2080
AciI CCGC 7 cut(s) 44, 800, 821, 1884, 2385, 2779, 3475
AclI AACGTT 1 cut(s) 1446
AclWI GGATC 3 cut(s) 683, 2301, 3098
AcoI YGGCCR 3 cut(s) 2733, 2752, 2780
AcsI RAATTY 8 cut(s) 346, 589, 1014, 1497, 1864, 1936, 3097, 3341
AcuI CTGAAG 7 cut(s) 453, 971, 2058, 2223, 2340, 2451, 3635
AcyI GRCGYC 1 cut(s) 533
AfaI GTAC 8 cut(s) 53, 665, 716, 1291, 1737, 2158, 2376, 3831
AflIII ACRYGT 1 cut(s) 2536
AjiI CACGTC 2 cut(s) 3614, 3784
AjuI GAANNNNNNNTTGG 4 cut(s) 894, 926, 3573, 3605
AleI CACNNNNGTG 1 cut(s) 279
AloI GAACNNNNNNTCC 2 cut(s) 1488, 1520
Alw21I GWGCWC 8 cut(s) 113, 155, 234, 290, 1269, 2443, 2698, 3807
Alw26I GTCTC 7 cut(s) 540, 1167, 1656, 1677, 2931, 3058, 3141
Alw44I GTGCAC 3 cut(s) 109, 286, 2439
AlwI GGATC 3 cut(s) 683, 2301, 3098
AlwNI CAGNNNCTG 3 cut(s) 476, 3107, 3569
Ama87I CYCGRG 2 cut(s) 2876, 3046
Aor13HI TCCGGA 1 cut(s) 2080
ApaLI GTGCAC 3 cut(s) 109, 286, 2439
ApoI RAATTY 8 cut(s) 346, 589, 1014, 1497, 1864, 1936, 3097, 3341
ArsI GACNNNNNNTTYG 2 cut(s) 2099, 2131
AseI ATTAAT 2 cut(s) 2955, 3158
Asp700I GAANNNNTTC 1 cut(s) 2927
AspA2I CCTAGG 2 cut(s) 2582, 2993
AspLEI GCGC 4 cut(s) 946, 1515, 2656, 3207
AsuC2I CCSGG 5 cut(s) 62, 2490, 2877, 2878, 3932
AsuII TTCGAA 2 cut(s) 587, 2859
AvaI CYCGRG 2 cut(s) 2876, 3046
AvaII GGWCC 4 cut(s) 2528, 2681, 3401, 3494
AvrII CCTAGG 2 cut(s) 2582, 2993
AxyI CCTNAGG 1 cut(s) 2523
BaeGI GKGCMC 5 cut(s) 113, 290, 1320, 2443, 2674
BaeI ACNNNNGTAYC 4 cut(s) 689, 722, 1772, 1805
BalI TGGCCA 2 cut(s) 2735, 2754
BanI GGYRCC 3 cut(s) 1000, 2321, 2671
BarI GAAGNNNNNNTAC 2 cut(s) 1896, 1928
BauI CACGAG 2 cut(s) 2946, 3264
BbsI GAAGAC 1 cut(s) 65
Bbv12I GWGCWC 8 cut(s) 113, 155, 234, 290, 1269, 2443, 2698, 3807
BbvCI CCTCAGC 3 cut(s) 1365, 1548, 1743
BceAI ACGGC 1 cut(s) 1727
BciVI GTATCC 2 cut(s) 687, 1800
BclI TGATCA 3 cut(s) 3424, 3700, 3877
BcnI CCSGG 5 cut(s) 62, 2490, 2877, 2878, 3932
BcoDI GTCTC 7 cut(s) 540, 1167, 1656, 1677, 2931, 3058, 3141
BfaI CTAG 6 cut(s) 2088, 2316, 2583, 2703, 2994, 3689
BfmI CTRYAG 5 cut(s) 354, 1179, 1956, 2364, 3108
BfoI RGCGCY 2 cut(s) 1516, 3208
BfuAI ACCTGC 3 cut(s) 819, 1288, 1924
BfuI GTATCC 2 cut(s) 687, 1800
BlnI CCTAGG 2 cut(s) 2582, 2993
BmcAI AGTACT 5 cut(s) 53, 665, 716, 1737, 2158
Bme18I GGWCC 4 cut(s) 2528, 2681, 3401, 3494
BmeT110I CYCGRG 2 cut(s) 2876, 3046
BmgBI CACGTC 2 cut(s) 3614, 3784
BmrI ACTGGG 1 cut(s) 1865
BmsI GCATC 8 cut(s) 190, 397, 1710, 2302, 2382, 2615, 3264, 3886
BmuI ACTGGG 1 cut(s) 1865
BoxI GACNNNNGTC 2 cut(s) 143, 965
BpiI GAAGAC 1 cut(s) 65
BpmI CTGGAG 5 cut(s) 597, 1452, 1493, 2183, 2408
Bpu10I CCTNAGC 3 cut(s) 1365, 1548, 1743
Bpu14I TTCGAA 2 cut(s) 587, 2859
BpuEI CTTGAG 6 cut(s) 585, 873, 1050, 1293, 2966, 3691
BpuMI CCSGG 5 cut(s) 62, 2490, 2877, 2878, 3932
Bsa29I ATCGAT 2 cut(s) 2959, 3316
BsaBI GATNNNNATC 1 cut(s) 3312
BsaHI GRCGYC 1 cut(s) 533
BsaI GGTCTC 2 cut(s) 2931, 3058
BsaWI WCCGGW 1 cut(s) 2080
BsaXI ACNNNNNCTCC 4 cut(s) 532, 562, 807, 837
Bse118I RCCGGY 2 cut(s) 1891, 2782
Bse21I CCTNAGG 1 cut(s) 2523
Bse3DI GCAATG 3 cut(s) 95, 262, 1527
Bse8I GATNNNNATC 1 cut(s) 3312
BseAI TCCGGA 1 cut(s) 2080
BseCI ATCGAT 2 cut(s) 2959, 3316
BseJI GATNNNNATC 1 cut(s) 3312
BseMI GCAATG 3 cut(s) 95, 262, 1527
BseRI GAGGAG 4 cut(s) 1053, 1782, 2649, 3034
BseSI GKGCMC 5 cut(s) 113, 290, 1320, 2443, 2674
BseX3I CGGCCG 1 cut(s) 2780
BseYI CCCAGC 2 cut(s) 1131, 2222
BsgI GTGCAG 3 cut(s) 419, 449, 1440
Bsh1285I CGRYCG 1 cut(s) 2783
BshNI GGYRCC 3 cut(s) 1000, 2321, 2671
BshVI ATCGAT 2 cut(s) 2959, 3316
BsiEI CGRYCG 1 cut(s) 2783
BsiHKAI GWGCWC 8 cut(s) 113, 155, 234, 290, 1269, 2443, 2698, 3807
BsiHKCI CYCGRG 2 cut(s) 2876, 3046
BsiSI CCGG 8 cut(s) 62, 1892, 2081, 2489, 2783, 2877, 2909, 3931
BslFI GGGAC 7 cut(s) 796, 1114, 1174, 1584, 1722, 2541, 3685
BsmAI GTCTC 7 cut(s) 540, 1167, 1656, 1677, 2931, 3058, 3141
BsmBI CGTCTC 2 cut(s) 540, 1167
BsmFI GGGAC 7 cut(s) 796, 1114, 1174, 1584, 1722, 2541, 3685
BsmI GAATGC 3 cut(s) 1180, 3787, 3853
Bso31I GGTCTC 2 cut(s) 2931, 3058
BsoBI CYCGRG 2 cut(s) 2876, 3046
Bsp119I TTCGAA 2 cut(s) 587, 2859
Bsp13I TCCGGA 1 cut(s) 2080
Bsp143I GATC 9 cut(s) 688, 2293, 2977, 3103, 3325, 3424, 3484, 3700, 3877
Bsp19I CCATGG 1 cut(s) 2749
BspACI CCGC 7 cut(s) 44, 800, 821, 1884, 2385, 2779, 3475
BspDI ATCGAT 2 cut(s) 2959, 3316
BspEI TCCGGA 1 cut(s) 2080
BspMAI CTGCAG 1 cut(s) 3112
BspMI ACCTGC 3 cut(s) 819, 1288, 1924
BspPI GGATC 3 cut(s) 683, 2301, 3098
BspT104I TTCGAA 2 cut(s) 587, 2859
BspT107I GGYRCC 3 cut(s) 1000, 2321, 2671
BspTNI GGTCTC 2 cut(s) 2931, 3058
BsrBI CCGCTC 1 cut(s) 823
BsrDI GCAATG 3 cut(s) 95, 262, 1527
BsrFI RCCGGY 2 cut(s) 1891, 2782
BssAI RCCGGY 2 cut(s) 1891, 2782
BssMI GATC 9 cut(s) 688, 2293, 2977, 3103, 3325, 3424, 3484, 3700, 3877
BssNI GRCGYC 1 cut(s) 533
BssSI CACGAG 2 cut(s) 2946, 3264
BssT1I CCWWGG 4 cut(s) 2582, 2749, 2993, 3125
Bst2BI CACGAG 2 cut(s) 2946, 3264
Bst6I CTCTTC 2 cut(s) 2136, 3027
BstACI GRCGYC 1 cut(s) 533
BstAPI GCANNNNNTGC 2 cut(s) 760, 2612
BstBI TTCGAA 2 cut(s) 587, 2859
BstDSI CCRYGG 1 cut(s) 2749
BstEII GGTNACC 2 cut(s) 3182, 3458
BstENI CCTNNNNNAGG 3 cut(s) 2466, 2664, 3058
BstH2I RGCGCY 2 cut(s) 1516, 3208
BstHHI GCGC 4 cut(s) 946, 1515, 2656, 3207
BstKTI GATC 9 cut(s) 691, 2296, 2980, 3106, 3328, 3427, 3487, 3703, 3880
BstMAI GTCTC 7 cut(s) 540, 1167, 1656, 1677, 2931, 3058, 3141
BstMBI GATC 9 cut(s) 688, 2293, 2977, 3103, 3325, 3424, 3484, 3700, 3877
BstMCI CGRYCG 1 cut(s) 2783
BstNSI RCATGY 5 cut(s) 1684, 2540, 2833, 3536, 3563
BstPAI GACNNNNGTC 2 cut(s) 143, 965
BstPI GGTNACC 2 cut(s) 3182, 3458
BstSFI CTRYAG 5 cut(s) 354, 1179, 1956, 2364, 3108
BstSLI GKGCMC 5 cut(s) 113, 290, 1320, 2443, 2674
BstV2I GAAGAC 1 cut(s) 65
BstX2I RGATCY 1 cut(s) 3103
BstXI CCANNNNNNTGG 1 cut(s) 3320
BstYI RGATCY 1 cut(s) 3103
BstZI CGGCCG 1 cut(s) 2780
Bsu15I ATCGAT 2 cut(s) 2959, 3316
Bsu36I CCTNAGG 1 cut(s) 2523
BsuI GTATCC 2 cut(s) 687, 1800
BsuTUI ATCGAT 2 cut(s) 2959, 3316
BtgI CCRYGG 1 cut(s) 2749
BtrI CACGTC 2 cut(s) 3614, 3784
BtsI GCAGTG 1 cut(s) 2233
BtsIMutI CAGTG 5 cut(s) 113, 534, 644, 1269, 2233
BveI ACCTGC 3 cut(s) 819, 1288, 1924
CaiI CAGNNNCTG 3 cut(s) 476, 3107, 3569
CfoI GCGC 4 cut(s) 946, 1515, 2656, 3207
Cfr10I RCCGGY 2 cut(s) 1891, 2782
Cfr9I CCCGGG 1 cut(s) 2876
ClaI ATCGAT 2 cut(s) 2959, 3316
Csp6I GTAC 8 cut(s) 52, 664, 715, 1290, 1736, 2157, 2375, 3830
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
CviQI GTAC 8 cut(s) 52, 664, 715, 1290, 1736, 2157, 2375, 3830
DpnI GATC 9 cut(s) 690, 2295, 2979, 3105, 3327, 3426, 3486, 3702, 3879
DpnII GATC 9 cut(s) 688, 2293, 2977, 3103, 3325, 3424, 3484, 3700, 3877
EaeI YGGCCR 3 cut(s) 2733, 2752, 2780
EagI CGGCCG 1 cut(s) 2780
Eam1104I CTCTTC 2 cut(s) 2136, 3027
EarI CTCTTC 2 cut(s) 2136, 3027
EclXI CGGCCG 1 cut(s) 2780
Eco130I CCWWGG 4 cut(s) 2582, 2749, 2993, 3125
Eco147I AGGCCT 2 cut(s) 2521, 2998
Eco31I GGTCTC 2 cut(s) 2931, 3058
Eco47I GGWCC 4 cut(s) 2528, 2681, 3401, 3494
Eco52I CGGCCG 1 cut(s) 2780
Eco57I CTGAAG 7 cut(s) 453, 971, 2058, 2223, 2340, 2451, 3635
Eco81I CCTNAGG 1 cut(s) 2523
Eco88I CYCGRG 2 cut(s) 2876, 3046
Eco91I GGTNACC 2 cut(s) 3182, 3458
EcoNI CCTNNNNNAGG 3 cut(s) 2466, 2664, 3058
EcoO109I RGGNCCY 6 cut(s) 299, 2471, 2528, 3401, 3494, 3566
EcoO65I GGTNACC 2 cut(s) 3182, 3458
EcoRI GAATTC 3 cut(s) 589, 1497, 3341
EcoT14I CCWWGG 4 cut(s) 2582, 2749, 2993, 3125
ErhI CCWWGG 4 cut(s) 2582, 2749, 2993, 3125
Esp3I CGTCTC 2 cut(s) 540, 1167
FalI AAGNNNNNCTT 2 cut(s) 2262, 2294
FaqI GGGAC 7 cut(s) 796, 1114, 1174, 1584, 1722, 2541, 3685
FauI CCCGC 1 cut(s) 37
FauNDI CATATG 2 cut(s) 1750, 3429
FbaI TGATCA 3 cut(s) 3424, 3700, 3877
FblI GTMKAC 1 cut(s) 81
FspBI CTAG 6 cut(s) 2088, 2316, 2583, 2703, 2994, 3689
FspI TGCGCA 1 cut(s) 945
GlaI GCGC 4 cut(s) 945, 1514, 2655, 3206
GsaI CCCAGC 2 cut(s) 1135, 2226
GsuI CTGGAG 5 cut(s) 597, 1452, 1493, 2183, 2408
HaeII RGCGCY 2 cut(s) 1516, 3208
HapII CCGG 8 cut(s) 62, 1892, 2081, 2489, 2783, 2877, 2909, 3931
HhaI GCGC 4 cut(s) 946, 1515, 2656, 3207
Hin1I GRCGYC 1 cut(s) 533
Hin6I GCGC 4 cut(s) 944, 1513, 2654, 3205
HinP1I GCGC 4 cut(s) 944, 1513, 2654, 3205
HincII GTYRAC 1 cut(s) 3757
HindII GTYRAC 1 cut(s) 3757
HpaII CCGG 8 cut(s) 62, 1892, 2081, 2489, 2783, 2877, 2909, 3931
Hpy166II GTNNAC 9 cut(s) 82, 111, 288, 1474, 2192, 2441, 2684, 3200, 3757
Hpy188III TCNNGA 9 cut(s) 148, 461, 1494, 1869, 2081, 2162, 2496, 3008, 3048
Hpy8I GTNNAC 9 cut(s) 82, 111, 288, 1474, 2192, 2441, 2684, 3200, 3757
Hpy99I CGWCG 2 cut(s) 149, 3231
HpyCH4IV ACGT 7 cut(s) 144, 533, 1288, 1446, 1597, 3613, 3783
HpySE526I ACGT 7 cut(s) 144, 533, 1288, 1446, 1597, 3613, 3783
Hsp92I GRCGYC 1 cut(s) 533
HspAI GCGC 4 cut(s) 944, 1513, 2654, 3205
Kpn2I TCCGGA 1 cut(s) 2080
KroI GCCGGC 2 cut(s) 1891, 2782
KroNI GCCGGC 2 cut(s) 1893, 2784
Ksp22I TGATCA 3 cut(s) 3424, 3700, 3877
Kzo9I GATC 9 cut(s) 688, 2293, 2977, 3103, 3325, 3424, 3484, 3700, 3877
LweI GCATC 8 cut(s) 190, 397, 1710, 2302, 2382, 2615, 3264, 3886
MaeI CTAG 6 cut(s) 2088, 2316, 2583, 2703, 2994, 3689
MaeII ACGT 7 cut(s) 144, 533, 1288, 1446, 1597, 3613, 3783
MaeIII GTNAC 8 cut(s) 102, 187, 523, 529, 1911, 3038, 3182, 3458
MalI GATC 9 cut(s) 690, 2295, 2979, 3105, 3327, 3426, 3486, 3702, 3879
MbiI CCGCTC 1 cut(s) 823
MboI GATC 9 cut(s) 688, 2293, 2977, 3103, 3325, 3424, 3484, 3700, 3877
MboII GAAGA 9 cut(s) 65, 2025, 2051, 2153, 2279, 2346, 2457, 2984, 3014
MflI RGATCY 1 cut(s) 3103
MlsI TGGCCA 2 cut(s) 2735, 2754
MluNI TGGCCA 2 cut(s) 2735, 2754
MlyI GAGTC 6 cut(s) 1342, 2093, 2123, 3084, 3382, 3701
MmeI TCCRAC 3 cut(s) 650, 1558, 1999
Mox20I TGGCCA 2 cut(s) 2735, 2754
MroI TCCGGA 1 cut(s) 2080
MroNI GCCGGC 2 cut(s) 1891, 2782
MroXI GAANNNNTTC 1 cut(s) 2927
MscI TGGCCA 2 cut(s) 2735, 2754
MseI TTAA 6 cut(s) 1977, 2507, 2898, 2955, 3158, 3866
MslI CAYNNNNRTG 5 cut(s) 279, 1635, 2754, 3168, 3387
Msp20I TGGCCA 2 cut(s) 2735, 2754
MspA1I CMGCKG 3 cut(s) 485, 2779, 3113
MspI CCGG 8 cut(s) 62, 1892, 2081, 2489, 2783, 2877, 2909, 3931
Mva1269I GAATGC 3 cut(s) 1180, 3787, 3853
NaeI GCCGGC 2 cut(s) 1893, 2784
NciI CCSGG 5 cut(s) 62, 2490, 2877, 2878, 3932
NcoI CCATGG 1 cut(s) 2749
NdeI CATATG 2 cut(s) 1750, 3429
NdeII GATC 9 cut(s) 688, 2293, 2977, 3103, 3325, 3424, 3484, 3700, 3877
NgoMIV GCCGGC 2 cut(s) 1891, 2782
NmuCI GTSAC 5 cut(s) 102, 187, 529, 3182, 3458
NsbI TGCGCA 1 cut(s) 945
NspI RCATGY 5 cut(s) 1684, 2540, 2833, 3536, 3563
NspV TTCGAA 2 cut(s) 587, 2859
OliI CACNNNNGTG 1 cut(s) 279
PaeI GCATGC 1 cut(s) 1684
PaeR7I CTCGAG 1 cut(s) 3046
PaqCI CACCTGC 1 cut(s) 1288
PasI CCCWGGG 1 cut(s) 1603
PceI AGGCCT 2 cut(s) 2521, 2998
PciI ACATGT 1 cut(s) 2536
PctI GAATGC 3 cut(s) 1180, 3787, 3853
PdiI GCCGGC 2 cut(s) 1893, 2784
PdmI GAANNNNTTC 1 cut(s) 2927
PflFI GACNNNGTC 1 cut(s) 3231
PflMI CCANNNNNTGG 9 cut(s) 176, 779, 1730, 2690, 3194, 3266, 3617, 3668, 3814
PfoI TCCNGGA 1 cut(s) 679
PleI GAGTC 6 cut(s) 1342, 2092, 2122, 3083, 3381, 3700
PpsI GAGTC 6 cut(s) 1342, 2092, 2122, 3083, 3381, 3700
PpuMI RGGWCCY 3 cut(s) 2528, 3401, 3494
PscI ACATGT 1 cut(s) 2536
PshAI GACNNNNGTC 2 cut(s) 143, 965
PshBI ATTAAT 2 cut(s) 2955, 3158
PsiI TTATAA 1 cut(s) 2640
Psp1406I AACGTT 1 cut(s) 1446
Psp5II RGGWCCY 3 cut(s) 2528, 3401, 3494
PspEI GGTNACC 2 cut(s) 3182, 3458
PspFI CCCAGC 2 cut(s) 1131, 2222
PspPPI RGGWCCY 3 cut(s) 2528, 3401, 3494
PsrI GAACNNNNNNTAC 2 cut(s) 1755, 1787
PstI CTGCAG 1 cut(s) 3112
PstNI CAGNNNCTG 3 cut(s) 476, 3107, 3569
PsuI RGATCY 1 cut(s) 3103
PsyI GACNNNGTC 1 cut(s) 3231
PvuII CAGCTG 2 cut(s) 485, 3113
RsaI GTAC 8 cut(s) 53, 665, 716, 1291, 1737, 2158, 2376, 3831
RsaNI GTAC 8 cut(s) 52, 664, 715, 1290, 1736, 2157, 2375, 3830
RseI CAYNNNNRTG 5 cut(s) 279, 1635, 2754, 3168, 3387
SaqAI TTAA 6 cut(s) 1977, 2507, 2898, 2955, 3158, 3866
Sau3AI GATC 9 cut(s) 688, 2293, 2977, 3103, 3325, 3424, 3484, 3700, 3877
ScaI AGTACT 5 cut(s) 53, 665, 716, 1737, 2158
SchI GAGTC 6 cut(s) 1342, 2093, 2123, 3084, 3382, 3701
SfaNI GCATC 8 cut(s) 190, 397, 1710, 2302, 2382, 2615, 3264, 3886
SfcI CTRYAG 5 cut(s) 354, 1179, 1956, 2364, 3108
Sfr274I CTCGAG 1 cut(s) 3046
SfuI TTCGAA 2 cut(s) 587, 2859
SinI GGWCC 4 cut(s) 2528, 2681, 3401, 3494
SlaI CTCGAG 1 cut(s) 3046
SmaI CCCGGG 1 cut(s) 2878
SmiMI CAYNNNNRTG 5 cut(s) 279, 1635, 2754, 3168, 3387
SmlI CTYRAG 7 cut(s) 600, 888, 1065, 1308, 2981, 3046, 3706
SmoI CTYRAG 7 cut(s) 600, 888, 1065, 1308, 2981, 3046, 3706
SphI GCATGC 1 cut(s) 1684
SseBI AGGCCT 2 cut(s) 2521, 2998
SsiI CCGC 7 cut(s) 44, 800, 821, 1884, 2385, 2779, 3475
SspI AATATT 1 cut(s) 1556
SspMI CTAG 6 cut(s) 2088, 2316, 2583, 2703, 2994, 3689
StuI AGGCCT 2 cut(s) 2521, 2998
StyI CCWWGG 4 cut(s) 2582, 2749, 2993, 3125
TaiI ACGT 7 cut(s) 147, 536, 1291, 1449, 1600, 3616, 3786
TatI WGTACW 6 cut(s) 51, 663, 714, 1735, 2156, 2374
TauI GCSGC 2 cut(s) 2388, 2782
Tru1I TTAA 6 cut(s) 1977, 2507, 2898, 2955, 3158, 3866
Tru9I TTAA 6 cut(s) 1977, 2507, 2898, 2955, 3158, 3866
TscAI CASTG 5 cut(s) 113, 534, 644, 1269, 2233
TseFI GTSAC 5 cut(s) 102, 187, 529, 3182, 3458
Tsp45I GTSAC 5 cut(s) 102, 187, 529, 3182, 3458
TspMI CCCGGG 1 cut(s) 2876
TspRI CASTG 5 cut(s) 113, 534, 644, 1269, 2233
Tth111I GACNNNGTC 1 cut(s) 3231
Van91I CCANNNNNTGG 9 cut(s) 176, 779, 1730, 2690, 3194, 3266, 3617, 3668, 3814
VneI GTGCAC 3 cut(s) 109, 286, 2439
VpaK11BI GGWCC 4 cut(s) 2528, 2681, 3401, 3494
VspI ATTAAT 2 cut(s) 2955, 3158
XagI CCTNNNNNAGG 3 cut(s) 2466, 2664, 3058
XapI RAATTY 8 cut(s) 346, 589, 1014, 1497, 1864, 1936, 3097, 3341
XceI RCATGY 5 cut(s) 1684, 2540, 2833, 3536, 3563
XcmI CCANNNNNNNNNTGG 1 cut(s) 677
XhoI CTCGAG 1 cut(s) 3046
XmaI CCCGGG 1 cut(s) 2876
XmaJI CCTAGG 2 cut(s) 2582, 2993
XmiI GTMKAC 1 cut(s) 81
XmnI GAANNNNTTC 1 cut(s) 2927
XspI CTAG 6 cut(s) 2088, 2316, 2583, 2703, 2994, 3689
ZraI GACGTC 1 cut(s) 534
ZrmI AGTACT 5 cut(s) 53, 665, 716, 1737, 2158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.