MD03G1079900.v1.1

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002401
Physical Location & Seq
Forward (+)
6437970 .. 6443837
5868 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1079900.v1.1.491

Sequence Viewer

Length: 3849 bp
ATGGGATTTGACAATGAGTGCATATTGAACATCCAGTCTCTTGCCGGAGAGTACTTTTGTCCGGTTTGTCGTCTGCTTGTTTACCCAACTGAAGCACTACAGTCGCAATGTACTCATCTGTATTGCAAGCCCTGTTTGACATACGTTGTGAGCACCACCCAAGCTTGCCCATATGATGGCTACTTAGTGACAGAAGCAGATGCCAAGCCACTCACGGAGTCAAATAAGGCCCTCTCTGATACCATTGGCAAAATACTAGTTCATTGCTTATTTCACAGGAGTGGATGCACATGGCAGGGACCATTATCCGAGTGCACACCTCATTGTTCTGGATGTGCCTTTGGCAATTCTCCTGTAGTATGCAACCGGTGTGGAGTCCAAATTGTGCATCGTCATGTGCAGGAACATGCACAAACATGTCCTGGTGTGCAGCCTCAAGCGCAGCAGGCAGAGGTTGCTCTGCACACTTCTGCTTCTGGCACATCAGCAGCTACTGCTGATCAATCCCAGGCTGCAATTCAAACAGGAACAACTACATCTCAGGCCCCGGTTTCTCAAACTACAAGTGCCACTGCACCTGGATCTGATCCAAATCAGAAAGTAAACTCAAGTTCTCAAGCTCAAGCGGCTGTGCCGACTGCTGATCAATGGTACCAGCAGCAGCAACAATATCAACAGTACTACCAGCTGTATCCTGGATATGACCCATATCAACAGCAATATCAGAACTACTATCCCTATCAACAGCCAGCAGTTCCACAACAACAGCAGGCTCAGCCACCATATGGGACCGTCCAGAATCAACCTCAGACATACTTGCAACCTCAGCCTCAAACTCAACCTCCGCCACAGCCCCAAACTCAACCTCTGCTACAGCCCCAAATTTCTCAAGCTCAAGCTCAACCCCAAATTCAGCCTCCAAATGGCCAGGCTCCTGTTGTGGCTCAAACTCAAAATCAGGTACAAGCCAACCAACAGCAGCAACTCCACCCGACAGTGCAGACTTACCCAGCAGCTCATGGGCAGACACAGCCATATTTCCAAGCTCCATCTCAGTCACAGCCTCATTCTCAACAGGTGCAAGTGCTTCCGTATCAGCAGCCCCAAATTCAGCAACATACCCAGGCCCATCTTCAACCGCAGCACCATCCCCTTTCTCAACCCCAGTCTCAGTCACAACCTCAGCAACAGGTACAGGTTCAACACCATCCCCAACTTCACCCTTCTCAGCCCATGAATGCTGCTGTCCAACCTCAGGCACAGCATCCACCATCTCATCCCGTGTCTGGCAATAACTCATATCCTCAACCTCACCTTCACCAACCAGCGCAACACAATATGTACGTGCAATCTCAAGGTGTGCCTCACTCTCAGTTACAGACTCCTGTTCAAATGCCGAATCAGTTTCCTCCACAACCCCCACTCATGCGTCCGCCTTCATCTCATACAACAATTCCAAACCAGCAGCAGCCAGCTTTGTTGCCTTCACCCGGTCAAGTCCAAAATATCAATCCTGCACAACAGCAGCCAGTTCCCTCTTATGCTCAGCAACCTGGAAATTCAGTCTACCAGCGCCCTATTATGCAAGCAGTTCAACAACCGATGCCTCAGCAGTATTTCCACCATCAGCCATATGTACAGCAGCAACCATCTACACAGTTACGTCCCCAGAGCCAATCTCATCTGTTCCCTCAACATATTCATGCCTCTACACAGTCTCAGGGCATTCAACATACTCAGTCTAATCTTGTCGGAAGACCTATGATGCCAAGTCATGGAGTACAACCTCAGCCTTATGGACAAACTGCTGTTCAGGTGAGGCCAATGCATTCTTCTGTAAACCATCCATCTACAAATCAGAATAATATGGTCAGAACAAACAACCCAGTGCAATCTGGAGCAAACTCAAGACCAACTATGTCTGAGAGGCAAGCGGAGAAAGAATCTGAGTCATCAGGTCAGAATGTTGCACATGATGCTGAATTTAAATCCGTGAAATCTGAGATGGGTATGAAGTCCGTTGATGATGAGCATAAACCTAGTGGTGGAGTTAAACCTAATCAGGGGGATACATCTTTCAAGGAGATTCCAGAGTCTAGGGGCTTGTCGGAGGCAGATCCAAATTTACATGCACTTGATAATGGAGGTTCTGTAAATATCCCAATGTTGAAGGAAGAGGGTATGGATAGTGCTATGGAGCACTCAAGTAATGGTAAATTGGGTGAAGTAGCTGAGGGCGCAAAAGAAATCCCATCGGAAGAAGCTCAACTTCATGAAGAACAGGGTCGAAGGTTGCAGAAGAATGCTAGTGGCATTCTGCAGCCTTCCAATGTACAAATACAAGCTGGCGGGGGTTCTCAAGCTGTATCTACTCCAGCTCCAACTGTTCAAGTACAAGCTGGTGGCTCTACACAACCTTCACATCCTGTATCAATACATCATGGACCTACTATACTCCCCCAAGGGCCTGGTGCACCTCCGCATCATACACAGGGGCCTGGGCAGCCCTTGGCTCATTTAAGACCTCAGGGACCTGCACATGTACCTGGACAGCCTTTTCATCCATCTGAGCATTTGCAGCCACCTGGTCCCAACCCGGGTTTTGGACCATCCTCTGGAAGGGGAGGTCAGCATGGTCCTCAAGGTGTACTACCTCCTTCCCATACAGGGGCTCCCAAGATGCAAGGTGAACCTGTTGGAGGCCCTCCATTTAGTGGAATGATGGCACCATATGGCCCTGAAGGTCATAGGAGTTACCAGCGGCCTGCCTTTCAGATTGATCAAGGAGCAATTGGACAGCCATCTGGTATCCCCCCAAATATGTTGAGAATGAATGGAACTCCAGGCCTCGACTCCTCATCAGTTCATGGGTTGAGGGATGAAAAGTTCAAGGCTTTTCCAGACGAACGAATGAATCCCTTCCCAGCAGATCCAACTTATCGTGGTTTTGACCGGGTAGCGTTTGAAGATGATCTCAAGCAATTCCCTAGGCCTTCGTATCTGGATTCTGATCCTGTTTCTAAATTTGGGAATTATTCATCAAGGCCCTTTGATAAGGGACCTCATGGGCTCAAGTATGATATGGGTCTGAATACAGATCCTGCTGCTGGAGATATTGGGAGAATGGAGCCTTCTCGCGGTCGTCCAGATTTTGTTGGACGACGCCATATGGATGTTTTGGCTCCCCGAAGCCCAGTTAGAGATTACCCTGCGATCCCATCTCATGGGTTTAGAGGCTTTGGCCCTGATGATTTTGATAGAAGAGACTTTCATCGATTTGGTGATCCACATGGAAATTCATTCCATGAGGGTAGGCTTTCTAATTTTCCTGGTCAATTTCGACGGGGTGAGTTTGAGGGGCCTGGTAATATGCGAATTGTTGATGGGCACCCTGGTCATCTTCGGAGGGGAGATCATTTGGGTCATCAGAATGTGCATAATCATTTGCATTTGAGAGAAACTATGGGTTTTGGTGATCGTCATCCACGGATTGGAGACATGACTGGACCTGGGAACTTTGAATCATTTAGAGGCAAGAGGACAAGCCATCCACGTCTTGGTGAGCCTGGATTTAGGAGCAGCTTTTCACTTCAGAGATTTCCAACTGATGGAGCTTACACAGGAGATCTAGAGTCTTTTGATCACTTAAGGAAAAGGAAACCAGCTAGTATGGGATGGTGCCGGATATGTAAAGTTGATTGTGAAACAGTTGAAGGTTTAGACTTGCATTCACAAACAAGGGAACACCAGAAGATGGCTATGGATATGGTGCGAACCATCAAGCAAAATGCCAAAAAGCAGAAACTAACCTCTGGTGATCAGTCCTTGGTAGAGGATGAAAATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000793 GO:0000794 GO:0001701 GO:0001756 GO:0001932 GO:0002020 GO:0002791 GO:0002793 GO:0003002 GO:0003007 GO:0003008 GO:0003012 GO:0003013 GO:0003015 GO:0003300 GO:0003674 GO:0003779 GO:0003824 GO:0004672 GO:0004674 GO:0004713 GO:0005198 GO:0005200 GO:0005488 GO:0005509 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005856 GO:0005859 GO:0005865 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006936 GO:0006941 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0007399 GO:0007417 GO:0007420 GO:0007507 GO:0007512 GO:0007517 GO:0008015 GO:0008092 GO:0008150 GO:0008152 GO:0008307 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009653 GO:0009790 GO:0009792 GO:0009887 GO:0009888 GO:0009893 GO:0009952 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010604 GO:0010628 GO:0010737 GO:0010927 GO:0014706 GO:0014866 GO:0014896 GO:0014897 GO:0015629 GO:0016043 GO:0016301 GO:0016310 GO:0016459 GO:0016460 GO:0016740 GO:0016772 GO:0016773 GO:0018108 GO:0018193 GO:0018212 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0021591 GO:0022607 GO:0023052 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030036 GO:0030154 GO:0030239 GO:0030240 GO:0030241 GO:0030506 GO:0031032 GO:0031033 GO:0031034 GO:0031323 GO:0031399 GO:0031430 GO:0031433 GO:0031672 GO:0031674 GO:0031974 GO:0031981 GO:0032268 GO:0032501 GO:0032502 GO:0032879 GO:0032880 GO:0032989 GO:0032991 GO:0033058 GO:0034622 GO:0035051 GO:0035265 GO:0035282 GO:0035556 GO:0035994 GO:0035995 GO:0036211 GO:0036379 GO:0040007 GO:0040011 GO:0042221 GO:0042325 GO:0042692 GO:0042802 GO:0042805 GO:0043009 GO:0043056 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043292 GO:0043412 GO:0043549 GO:0043621 GO:0043933 GO:0044057 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044449 GO:0044464 GO:0044877 GO:0045214 GO:0045859 GO:0046872 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048589 GO:0048644 GO:0048646 GO:0048729 GO:0048731 GO:0048738 GO:0048739 GO:0048747 GO:0048769 GO:0048856 GO:0048869 GO:0050708 GO:0050714 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0050982 GO:0051015 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051146 GO:0051171 GO:0051174 GO:0051222 GO:0051223 GO:0051239 GO:0051246 GO:0051338 GO:0051371 GO:0051393 GO:0051592 GO:0051606 GO:0051716 GO:0055001 GO:0055002 GO:0055003 GO:0055006 GO:0055007 GO:0055008 GO:0055013 GO:0060047 GO:0060048 GO:0060255 GO:0060322 GO:0060415 GO:0060419 GO:0060429 GO:0060537 GO:0061053 GO:0061061 GO:0065003 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070925 GO:0071688 GO:0071704 GO:0071840 GO:0072359 GO:0080090 GO:0090087 GO:0090257 GO:0097435 GO:0097493 GO:0099080 GO:0099081 GO:0099512 GO:0140096 GO:1901077 GO:1901564 GO:1901897 GO:1903530 GO:1903532 GO:1904951
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1283

Amino Acids

140.73

Weight (kDa)

6.91

Isoelectric Point (pI)

60.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2542
Acc65I GGTACC 1 cut(s) 651
AccB1I GGYRCC 4 cut(s) 651, 2725, 3387, 3678
AccI GTMKAC 1 cut(s) 1566
AccII CGCG 1 cut(s) 3138
AciI CCGC 9 cut(s) 626, 845, 1139, 1433, 1934, 2349, 2480, 2761, 3138
AclWI GGATC 8 cut(s) 581, 589, 2111, 2924, 3005, 3092, 3208, 3278
AcoI YGGCCR 1 cut(s) 925
AcsI RAATTY 8 cut(s) 882, 909, 1107, 1558, 1982, 2122, 3023, 3295
AcuI CTGAAG 3 cut(s) 111, 2760, 3575
AcyI GRCGYC 1 cut(s) 3163
AflII CTTAAG 1 cut(s) 3646
AflIII ACRYGT 2 cut(s) 416, 2539
AgeI ACCGGT 1 cut(s) 366
AhlI ACTAGT 1 cut(s) 256
AjiI CACGTC 1 cut(s) 3554
AjuI GAANNNNNNNTTGG 2 cut(s) 1816, 1848
AleI CACNNNNGTG 1 cut(s) 279
AloI GAACNNNNNNTCC 2 cut(s) 2870, 2902
Alw21I GWGCWC 4 cut(s) 155, 317, 2202, 2476
Alw26I GTCTC 5 cut(s) 42, 1173, 1722, 3258, 3489
Alw44I GTGCAC 2 cut(s) 313, 2472
AlwI GGATC 8 cut(s) 581, 589, 2111, 2924, 3005, 3092, 3208, 3278
AlwNI CAGNNNCTG 2 cut(s) 494, 3101
Ama87I CYCGRG 1 cut(s) 2596
ApaLI GTGCAC 2 cut(s) 313, 2472
ApoI RAATTY 8 cut(s) 882, 909, 1107, 1558, 1982, 2122, 3023, 3295
AsiGI ACCGGT 1 cut(s) 366
Asp700I GAANNNNTTC 1 cut(s) 2918
Asp718I GGTACC 1 cut(s) 651
AspA2I CCTAGG 1 cut(s) 2987
AspLEI GCGC 4 cut(s) 442, 1330, 1575, 2240
AsuC2I CCSGG 5 cut(s) 548, 1491, 2597, 2598, 2954
AvaI CYCGRG 1 cut(s) 2596
AvaII GGWCC 9 cut(s) 299, 789, 2444, 2531, 2588, 2606, 2636, 3059, 3506
AvrII CCTAGG 1 cut(s) 2987
AxyI CCTNAGG 2 cut(s) 1254, 2526
BaeGI GKGCMC 3 cut(s) 317, 2476, 3390
BalI TGGCCA 1 cut(s) 927
BanI GGYRCC 4 cut(s) 651, 2725, 3387, 3678
BanII GRGCYC 2 cut(s) 2674, 3072
BbsI GAAGAC 1 cut(s) 1762
Bbv12I GWGCWC 4 cut(s) 155, 317, 2202, 2476
BbvCI CCTCAGC 5 cut(s) 825, 1182, 1608, 1788, 2232
BcgI CGANNNNNNTGC 2 cut(s) 84, 118
BciVI GTATCC 3 cut(s) 702, 2062, 2819
BclI TGATCA 5 cut(s) 499, 643, 2779, 3640, 3817
BcnI CCSGG 5 cut(s) 548, 1491, 2597, 2598, 2954
BcoDI GTCTC 5 cut(s) 42, 1173, 1722, 3258, 3489
BcuI ACTAGT 1 cut(s) 256
BfaI CTAG 7 cut(s) 257, 2040, 2097, 2307, 2988, 3629, 3666
BfmI CTRYAG 4 cut(s) 98, 354, 872, 2318
BfoI RGCGCY 1 cut(s) 1576
BfrI CTTAAG 1 cut(s) 3646
BfuAI ACCTGC 1 cut(s) 2542
BfuI GTATCC 3 cut(s) 702, 2062, 2819
BglII AGATCT 1 cut(s) 3625
BlnI CCTAGG 1 cut(s) 2987
BlpI GCTNAGC 2 cut(s) 774, 1545
BmcAI AGTACT 2 cut(s) 53, 680
Bme18I GGWCC 9 cut(s) 299, 789, 2444, 2531, 2588, 2606, 2636, 3059, 3506
BmeT110I CYCGRG 1 cut(s) 2596
BmgBI CACGTC 1 cut(s) 3554
BmrI ACTGGG 3 cut(s) 1159, 1880, 3188
BmsI GCATC 9 cut(s) 190, 275, 397, 1273, 1593, 1755, 1966, 2491, 2670
BmuI ACTGGG 3 cut(s) 1159, 1880, 3188
BpiI GAAGAC 1 cut(s) 1762
BplI GAGNNNNNCTC 2 cut(s) 1663, 1695
BpmI CTGGAG 4 cut(s) 1917, 2358, 2826, 3129
Bpu10I CCTNAGC 5 cut(s) 825, 1182, 1608, 1788, 2232
Bpu1102I GCTNAGC 2 cut(s) 774, 1545
BpuMI CCSGG 5 cut(s) 548, 1491, 2597, 2598, 2954
Bsa29I ATCGAT 1 cut(s) 3274
BsaAI YACGTR 1 cut(s) 1345
BsaBI GATNNNNATC 3 cut(s) 591, 3009, 3480
BsaHI GRCGYC 1 cut(s) 3163
BsaWI WCCGGW 2 cut(s) 61, 366
BsaXI ACNNNNNCTCC 8 cut(s) 826, 856, 2136, 2166, 2362, 2392, 2656, 2686
Bse118I RCCGGY 1 cut(s) 366
Bse1I ACTGG 6 cut(s) 34, 1165, 1529, 1886, 3194, 3508
Bse21I CCTNAGG 2 cut(s) 1254, 2526
Bse3DI GCAATG 2 cut(s) 113, 262
Bse8I GATNNNNATC 3 cut(s) 591, 3009, 3480
BseCI ATCGAT 1 cut(s) 3274
BseJI GATNNNNATC 3 cut(s) 591, 3009, 3480
BseMI GCAATG 2 cut(s) 113, 262
BseNI ACTGG 6 cut(s) 34, 1165, 1529, 1886, 3194, 3508
BseRI GAGGAG 1 cut(s) 2845
BseSI GKGCMC 3 cut(s) 317, 2476, 3390
BseYI CCCAGC 2 cut(s) 1009, 2923
BsgI GTGCAG 7 cut(s) 419, 446, 449, 558, 1019, 1500, 2520
Bsh1236I CGCG 1 cut(s) 3138
Bsh1285I CGRYCG 1 cut(s) 3142
BshNI GGYRCC 4 cut(s) 651, 2725, 3387, 3678
BshTI ACCGGT 1 cut(s) 366
BshVI ATCGAT 1 cut(s) 3274
BsiEI CGRYCG 1 cut(s) 3142
BsiHKAI GWGCWC 4 cut(s) 155, 317, 2202, 2476
BsiHKCI CYCGRG 1 cut(s) 2596
BsiSI CCGG 8 cut(s) 45, 62, 367, 548, 1491, 2597, 2953, 3682
BslFI GGGAC 6 cut(s) 312, 802, 1650, 2544, 2574, 3072
BsmAI GTCTC 5 cut(s) 42, 1173, 1722, 3258, 3489
BsmFI GGGAC 6 cut(s) 312, 802, 1650, 2544, 2574, 3072
BsmI GAATGC 6 cut(s) 1243, 1725, 1828, 2308, 2313, 3727
BsoBI CYCGRG 1 cut(s) 2596
Bsp1286I GDGCHC 7 cut(s) 155, 317, 2202, 2476, 2674, 3072, 3390
Bsp1407I TGTACA 2 cut(s) 1636, 2332
Bsp1720I GCTNAGC 2 cut(s) 774, 1545
BspACI CCGC 9 cut(s) 626, 845, 1139, 1433, 1934, 2349, 2480, 2761, 3138
BspDI ATCGAT 1 cut(s) 3274
BspFNI CGCG 1 cut(s) 3138
BspHI TCATGA 1 cut(s) 2272
BspMAI CTGCAG 1 cut(s) 2322
BspMI ACCTGC 1 cut(s) 2542
BspPI GGATC 8 cut(s) 581, 589, 2111, 2924, 3005, 3092, 3208, 3278
BspT107I GGYRCC 4 cut(s) 651, 2725, 3387, 3678
BspTI CTTAAG 1 cut(s) 3646
BsrDI GCAATG 2 cut(s) 113, 262
BsrFI RCCGGY 1 cut(s) 366
BsrGI TGTACA 2 cut(s) 1636, 2332
BsrI ACTGG 6 cut(s) 34, 1165, 1529, 1886, 3194, 3508
BssAI RCCGGY 1 cut(s) 366
BssNI GRCGYC 1 cut(s) 3163
BssT1I CCWWGG 4 cut(s) 2461, 2508, 2987, 3825
Bst4CI ACNGT 8 cut(s) 102, 678, 793, 997, 1659, 1716, 2386, 3709
Bst6I CTCTTC 2 cut(s) 2169, 3256
BstACI GRCGYC 1 cut(s) 3163
BstAFI CTTAAG 1 cut(s) 3646
BstAPI GCANNNNNTGC 3 cut(s) 455, 494, 1976
BstAUI TGTACA 2 cut(s) 1636, 2332
BstBAI YACGTR 1 cut(s) 1345
BstDSI CCRYGG 1 cut(s) 3485
BstENI CCTNNNNNAGG 2 cut(s) 2617, 2697
BstFNI CGCG 1 cut(s) 3138
BstH2I RGCGCY 1 cut(s) 1576
BstHHI GCGC 4 cut(s) 442, 1330, 1575, 2240
BstMAI GTCTC 5 cut(s) 42, 1173, 1722, 3258, 3489
BstMCI CGRYCG 1 cut(s) 3142
BstNSI RCATGY 4 cut(s) 410, 420, 2132, 2543
BstSFI CTRYAG 4 cut(s) 98, 354, 872, 2318
BstSLI GKGCMC 3 cut(s) 317, 2476, 3390
BstUI CGCG 1 cut(s) 3138
BstV2I GAAGAC 1 cut(s) 1762
BstX2I RGATCY 5 cut(s) 581, 2116, 2929, 3097, 3625
BstXI CCANNNNNNTGG 1 cut(s) 2468
BstYI RGATCY 5 cut(s) 581, 2116, 2929, 3097, 3625
Bsu15I ATCGAT 1 cut(s) 3274
Bsu36I CCTNAGG 2 cut(s) 1254, 2526
BsuI GTATCC 3 cut(s) 702, 2062, 2819
BsuTUI ATCGAT 1 cut(s) 3274
BtgI CCRYGG 1 cut(s) 3485
BtrI CACGTC 1 cut(s) 3554
BtsI GCAGTG 1 cut(s) 570
BtsIMutI CAGTG 3 cut(s) 570, 1002, 1893
BveI ACCTGC 1 cut(s) 2542
CaiI CAGNNNCTG 2 cut(s) 494, 3101
CciI TCATGA 1 cut(s) 2272
CfoI GCGC 4 cut(s) 442, 1330, 1575, 2240
Cfr10I RCCGGY 1 cut(s) 366
Cfr9I CCCGGG 1 cut(s) 2596
ClaI ATCGAT 1 cut(s) 3274
CseI GACGC 2 cut(s) 1418, 3171
CsiI ACCWGGT 1 cut(s) 2584
CspAI ACCGGT 1 cut(s) 366
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
DraI TTTAAA 1 cut(s) 1987
EaeI YGGCCR 1 cut(s) 925
Eam1104I CTCTTC 2 cut(s) 2169, 3256
EarI CTCTTC 2 cut(s) 2169, 3256
EciI GGCGGA 2 cut(s) 834, 1422
Eco130I CCWWGG 4 cut(s) 2461, 2508, 2987, 3825
Eco147I AGGCCT 2 cut(s) 2847, 2992
Eco24I GRGCYC 2 cut(s) 2674, 3072
Eco47I GGWCC 9 cut(s) 299, 789, 2444, 2531, 2588, 2606, 2636, 3059, 3506
Eco57I CTGAAG 3 cut(s) 111, 2760, 3575
Eco81I CCTNAGG 2 cut(s) 1254, 2526
Eco88I CYCGRG 1 cut(s) 2596
EcoNI CCTNNNNNAGG 2 cut(s) 2617, 2697
EcoO109I RGGNCCY 9 cut(s) 229, 544, 2465, 2495, 2531, 2702, 3045, 3059, 3359
EcoT14I CCWWGG 4 cut(s) 2461, 2508, 2987, 3825
EcoT22I ATGCAT 1 cut(s) 1830
EcoT38I GRGCYC 2 cut(s) 2674, 3072
ErhI CCWWGG 4 cut(s) 2461, 2508, 2987, 3825
FalI AAGNNNNNCTT 2 cut(s) 2253, 2285
FaqI GGGAC 6 cut(s) 312, 802, 1650, 2544, 2574, 3072
FauI CCCGC 1 cut(s) 2342
FauNDI CATATG 5 cut(s) 172, 784, 1633, 2731, 3168
FbaI TGATCA 5 cut(s) 499, 643, 2779, 3640, 3817
FblI GTMKAC 1 cut(s) 1566
FriOI GRGCYC 2 cut(s) 2674, 3072
FspBI CTAG 7 cut(s) 257, 2040, 2097, 2307, 2988, 3629, 3666
GlaI GCGC 4 cut(s) 441, 1329, 1574, 2239
GsaI CCCAGC 2 cut(s) 1013, 2927
GsuI CTGGAG 4 cut(s) 1917, 2358, 2826, 3129
HaeII RGCGCY 1 cut(s) 1576
HapII CCGG 8 cut(s) 45, 62, 367, 548, 1491, 2597, 2953, 3682
HgaI GACGC 2 cut(s) 1418, 3171
HhaI GCGC 4 cut(s) 442, 1330, 1575, 2240
Hin1I GRCGYC 1 cut(s) 3163
Hin6I GCGC 4 cut(s) 440, 1328, 1573, 2238
HinP1I GCGC 4 cut(s) 440, 1328, 1573, 2238
HindIII AAGCTT 1 cut(s) 162
HpaII CCGG 8 cut(s) 45, 62, 367, 548, 1491, 2597, 2953, 3682
Hpy166II GTNNAC 8 cut(s) 82, 315, 604, 1567, 1840, 2474, 2648, 2690
Hpy8I GTNNAC 8 cut(s) 82, 315, 604, 1567, 1840, 2474, 2648, 2690
Hpy99I CGWCG 2 cut(s) 3165, 3345
HpyCH4III ACNGT 8 cut(s) 102, 678, 793, 997, 1659, 1716, 2386, 3709
HpyCH4IV ACGT 4 cut(s) 144, 1344, 1663, 3553
HpySE526I ACGT 4 cut(s) 144, 1344, 1663, 3553
Hsp92I GRCGYC 1 cut(s) 3163
HspAI GCGC 4 cut(s) 440, 1328, 1573, 2238
KpnI GGTACC 1 cut(s) 655
Ksp22I TGATCA 5 cut(s) 499, 643, 2779, 3640, 3817
LweI GCATC 9 cut(s) 190, 275, 397, 1273, 1593, 1755, 1966, 2491, 2670
MabI ACCWGGT 1 cut(s) 2584
MaeI CTAG 7 cut(s) 257, 2040, 2097, 2307, 2988, 3629, 3666
MaeII ACGT 4 cut(s) 144, 1344, 1663, 3553
MaeIII GTNAC 6 cut(s) 187, 1056, 1173, 1374, 1659, 2753
MfeI CAATTG 1 cut(s) 2790
MflI RGATCY 5 cut(s) 581, 2116, 2929, 3097, 3625
MhlI GDGCHC 7 cut(s) 155, 317, 2202, 2476, 2674, 3072, 3390
MlsI TGGCCA 1 cut(s) 927
MluNI TGGCCA 1 cut(s) 927
MlyI GAGTC 7 cut(s) 227, 384, 1375, 1958, 2102, 2846, 3641
MmeI TCCRAC 8 cut(s) 1273, 1732, 2088, 2405, 2677, 2957, 3136, 3626
Mox20I TGGCCA 1 cut(s) 927
Mph1103I ATGCAT 1 cut(s) 1830
MroXI GAANNNNTTC 1 cut(s) 2918
MscI TGGCCA 1 cut(s) 927
MseI TTAA 4 cut(s) 1986, 2052, 2519, 3647
MslI CAYNNNNRTG 6 cut(s) 279, 393, 415, 1701, 3171, 3429
Msp20I TGGCCA 1 cut(s) 927
MspA1I CMGCKG 2 cut(s) 688, 2761
MspCI CTTAAG 1 cut(s) 3646
MspI CCGG 8 cut(s) 45, 62, 367, 548, 1491, 2597, 2953, 3682
MunI CAATTG 1 cut(s) 2790
Mva1269I GAATGC 6 cut(s) 1243, 1725, 1828, 2308, 2313, 3727
MvnI CGCG 1 cut(s) 3138
NciI CCSGG 5 cut(s) 548, 1491, 2597, 2598, 2954
NdeI CATATG 5 cut(s) 172, 784, 1633, 2731, 3168
NmuCI GTSAC 3 cut(s) 187, 1056, 1173
NsiI ATGCAT 1 cut(s) 1830
NspI RCATGY 4 cut(s) 410, 420, 2132, 2543
OliI CACNNNNGTG 1 cut(s) 279
PagI TCATGA 1 cut(s) 2272
PceI AGGCCT 2 cut(s) 2847, 2992
PciI ACATGT 2 cut(s) 416, 2539
PctI GAATGC 6 cut(s) 1243, 1725, 1828, 2308, 2313, 3727
PdmI GAANNNNTTC 1 cut(s) 2918
PfeI GAWTC 7 cut(s) 799, 1399, 1943, 2086, 2914, 3005, 3521
PfoI TCCNGGA 1 cut(s) 694
PinAI ACCGGT 1 cut(s) 366
PleI GAGTC 7 cut(s) 226, 383, 1375, 1957, 2101, 2846, 3640
PpsI GAGTC 7 cut(s) 226, 383, 1375, 1957, 2101, 2846, 3640
Ppu21I YACGTR 1 cut(s) 1345
PpuMI RGGWCCY 2 cut(s) 2531, 3059
PscI ACATGT 2 cut(s) 416, 2539
Psp5II RGGWCCY 2 cut(s) 2531, 3059
PspFI CCCAGC 2 cut(s) 1009, 2923
PspPPI RGGWCCY 2 cut(s) 2531, 3059
PstI CTGCAG 1 cut(s) 2322
PstNI CAGNNNCTG 2 cut(s) 494, 3101
PsuI RGATCY 5 cut(s) 581, 2116, 2929, 3097, 3625
PvuII CAGCTG 1 cut(s) 688
RseI CAYNNNNRTG 6 cut(s) 279, 393, 415, 1701, 3171, 3429
SaqAI TTAA 4 cut(s) 1986, 2052, 2519, 3647
ScaI AGTACT 2 cut(s) 53, 680
SchI GAGTC 7 cut(s) 227, 384, 1375, 1958, 2102, 2846, 3641
SduI GDGCHC 7 cut(s) 155, 317, 2202, 2476, 2674, 3072, 3390
SexAI ACCWGGT 1 cut(s) 2584
SfaNI GCATC 9 cut(s) 190, 275, 397, 1273, 1593, 1755, 1966, 2491, 2670
SfcI CTRYAG 4 cut(s) 98, 354, 872, 2318
SinI GGWCC 9 cut(s) 299, 789, 2444, 2531, 2588, 2606, 2636, 3059, 3506
SmaI CCCGGG 1 cut(s) 2598
SmiI ATTTAAAT 1 cut(s) 1987
SmiMI CAYNNNNRTG 6 cut(s) 279, 393, 415, 1701, 3171, 3429
SpeI ACTAGT 1 cut(s) 256
SseBI AGGCCT 2 cut(s) 2847, 2992
SsiI CCGC 9 cut(s) 626, 845, 1139, 1433, 1934, 2349, 2480, 2761, 3138
SspMI CTAG 7 cut(s) 257, 2040, 2097, 2307, 2988, 3629, 3666
StuI AGGCCT 2 cut(s) 2847, 2992
StyI CCWWGG 4 cut(s) 2461, 2508, 2987, 3825
SwaI ATTTAAAT 1 cut(s) 1987
TaaI ACNGT 8 cut(s) 102, 678, 793, 997, 1659, 1716, 2386, 3709
TaiI ACGT 4 cut(s) 147, 1347, 1666, 3556
TaqI TCGA 4 cut(s) 2287, 2850, 3274, 3340
TatI WGTACW 8 cut(s) 51, 110, 678, 1636, 1780, 2332, 2392, 2647
TauI GCSGC 2 cut(s) 629, 2764
TfiI GAWTC 7 cut(s) 799, 1399, 1943, 2086, 2914, 3005, 3521
Tru1I TTAA 4 cut(s) 1986, 2052, 2519, 3647
Tru9I TTAA 4 cut(s) 1986, 2052, 2519, 3647
TscAI CASTG 3 cut(s) 577, 1002, 1893
TseFI GTSAC 3 cut(s) 187, 1056, 1173
Tsp45I GTSAC 3 cut(s) 187, 1056, 1173
TspGWI ACGGA 5 cut(s) 230, 1080, 1981, 2008, 3502
TspMI CCCGGG 1 cut(s) 2596
TspRI CASTG 3 cut(s) 577, 1002, 1893
Vha464I CTTAAG 1 cut(s) 3646
VneI GTGCAC 2 cut(s) 313, 2472
VpaK11BI GGWCC 9 cut(s) 299, 789, 2444, 2531, 2588, 2606, 2636, 3059, 3506
XagI CCTNNNNNAGG 2 cut(s) 2617, 2697
XapI RAATTY 8 cut(s) 882, 909, 1107, 1558, 1982, 2122, 3023, 3295
XbaI TCTAGA 1 cut(s) 3628
XceI RCATGY 4 cut(s) 410, 420, 2132, 2543
XcmI CCANNNNNNNNNTGG 2 cut(s) 692, 3554
XmaI CCCGGG 1 cut(s) 2596
XmaJI CCTAGG 1 cut(s) 2987
XmiI GTMKAC 1 cut(s) 1566
XmnI GAANNNNTTC 1 cut(s) 2918
XspI CTAG 7 cut(s) 257, 2040, 2097, 2307, 2988, 3629, 3666
ZrmI AGTACT 2 cut(s) 53, 680
Zsp2I ATGCAT 1 cut(s) 1830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.