Rw5G041470

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002401
Physical Location & Seq
Reverse (-)
73019588 .. 73023971
4384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G041470.1

Sequence Viewer

Length: 3936 bp
ATGGGATTTGATAATGAGTGCATACTGAACATCCAATCTCTTGCGGGAGAGTACTTTTGCCCGGTCTGTCGTCTTCTTGTCTACCCAAATGAAGCATTGCAGTCACAGTGCACTCATCTATACTGTAAGCCCTGTTTGACATACGTCGTGAGCACTACCAAAGCGTGTCCATACGATGGCTACTTAGTGACAGAAGCTGATGCGAAGCCCCTCGTTGAATCAAATAAAGTGCTCGCTGAAACCATAGGGAAAATAGCAGTTCATTGCCTGTATCACAGGAGTGGGTGCACATGGCAGGGGCCTTTATCTGATTGCACATCTCATTGTTCTGGTTGTGCCTTTGGAAATTCTCCTGTAGTCTGCAACAGGTGTGGAATCCAAATTGTGCATCGTCAAGTGCAGGAACACGCACAAAGTTGCCCTGGTGTGCAGCCCCAAGCACAGCAGGCAGAGGGTGTTCCAGACACTTCAGCCTCTGGCACAGCTGCTACTGCCAATCAGAACCAGGCTGCCACTCAGACGGTTACAGTGACGTCTCAGACACAGGCTCCCCAAACTGCAAGTGCCACTATACCTGGAGCTGATTCGAATTCTCAGGCTCAAGCTGTTGTTCAAGCTGCTGCTCCGACTGCTGACCAGTGGTATCAGCAACAATATCAGCAGTACTACCAGCAGTATCCTGGATATGATCCATACCAACAACAGTATCAGCAGTACTATCCCTATCAGCAGCCAGCAATTCCACAACCACAGCAGCCTCTGCAGGCTAATCCACCCTATGGGACTGTTCAGCCTCAACCGCAGGCATACCTGCAACCTCCGCTCCTACCTCAACCTCAACCTCAACCTCAACCACAGACCCAACCATCTCAATCTCAAGCTCAACCCCAACCACAAGTTCAGCCCCCAATTATGCAGGCTCCTATGGCTGCGCATACTCAAAACCAGACATCAGTCAATCAACAACTTCAACTTCAGCCTGCTATGGCACCACATTCACAAATTCAGGCACAGTCTTACCCATCAGCTCATGGGCAGGCTCAACTTCCTCCTCAAGCATATTTCCAAGTCCAAATGCCTCAATATCAGCAGCCACAAGTTCAGCAGCTTACACAGTCCCAGCTTCAACAGAATCCCCAACTTCACCCGTCTCAGCCCATGAATGCTACAGTCCAGTCCCAGACACAGCTCCCATCATCTAATTCATCGACTGGCAATCATTTGTTCCCTCAATCTCACCTTCCTCAACCAGTGCTCTCAGCAGCCTCCCAACAACGTACTATGCAGGTGCAATCTCAAGGAGTGCCCAACTCTCAGTCGCAAAACCATGTTCAGACTCAGATTCAGTTTCCTCAGCAACCCCTTTTGCGACCACCCCCGTTTCAAACAATAGTTCCAAACCAGCAGCAGACAGCCTTGTTGCCTTCACCTAACGTTATCTCTGCACAACAGCCTCCAGTTCACTCCTTTGCTCAACAATCTGGAATTCCTGTTCTCCAGCGCCCTGCAATGCAGCCAGTCCAACAGCTAAATCCTCAGCAATATTTCCACATACAACAGACACCCCCCACATTGTCGCAATTACGTCCCCAGGGTCAATCTCAATCATTCCCACAACATATTCGTGCCTCGAACCAGTCTCAGCAGAATGTTGTATTGTCTCAGGGCATGCAACATATTCAGCCCTCAAATCTTGTGGGACGGCCTATGATGCCAAGTCATGGAGTACTACCTCAGCCATATGCACAAACAGTAGGAGTTCTGCCAAGACCAATGTATCCTTCTCTAAACCATCAATCTACAAATCAGAATAATATAGGCAGAACCAACAACCAAGTGCAACTGGGAGCAAATTCAAGACCAACAATGACCGCAAGGCCGGCAGAGAAAGAAGCTGAGTTACCTGCTAATGATGTTGCACAGAATTTGGGTGTATTGTCTGCTGTAGGTGCTGATTCCGAGGTTAAAACTGTGAAATCTGAGGTGGATATGAAGTCCATTGATGTTGGAAATAAACCTTCTTCTGAAGATAGAAGTTATCAGGGTAATACATCTGCCAAAGAGATTCCGGAGTCTAGGGGGATGTTGGGGGCGAATGAAGAGTCTGAAAGTAAGCCAACATTGAAGGAAGAGGGTGTAAATAGTACTTTGGAGGATTCAAGTAATGATAAATTGGGTGAACTTGTAGCTGAAGGTGCAAAAGATGTTCCCAGCAGTGGCATGAAACAGGGGGAGCGTAAAGAAATGCCATCAGAAGAAGCACAACTTCATGGAGTAAAGGATCAAAAATTGCAAAAAGATGCTAGTGGCACCGAGGAGGGTTCCCAAACTGTTTCTACTTCTTCAGCTCCTATTGGTCAAGTACAAGCTGGCGGCTTGATGCAACCTTCATATCCTGGCTCTGCTATACTCCAGCAAAGGCCAGGTGCACCTCCTTCGTTACAAGTGCCTTCTTCAGGGCCTCAACATCATTTACCGGGTTCTGGACAACCCTTAATTCATGCAAGGCCTCAGGGACCTGGACATGTGCCTGGGCAGGCTTCTCATCTATCTGAGCATTTTCAATCACCTAGGGGAAACCTGGGATTTGCAGCATCATCTGCAAGTGCAAGTCAGCATGGGCCTTATAACCAAAGCCATGCGCCTCCTCATCCAGGGGCACCCAGAGGTCCACCATTTGGAGCACCCCCTAGTGCTTTTGATTTTCATGGGGGTATAATGGCCAGAGCAGCACCCCATGGCCATGAAGGTCAGAGGGGTCAGCAGCGGCCGGCTTTTCAGATGGAACAGGGAGCAACTGGACAGCCATCCTCAAACATGCTCAGAATGAATGGAAATCCAGGCTTCGAATCCTTATCAACTCCCGGGTTGAGGGATGAAAGATTTAAGGCTTTGCCGGATGGGAGGTTGAATCCCTTCCCAGGAGACCCAACTCGTGTGATTAATCGATTAGGGTTTGAAGATGATCTCAAGCAATTCCCTAGGCCTTCTTATCTGGATTCTGAGCCTCTTCCAAAATTAGGGAGTTACTCCTCTAGAGCCTTCGATAGGAGACCTCTTGGAGTCAATTATGATACTCGTCTAAATTCAGATCCTGCAGCTGGCTCTGCTCCAAGGTTCCTGTCTCCCTATGGTCATGGTGGATTAATTCATGCCAATGATACAGGTGGTCACCCAGATTTTGGTGGACAGCGCCTTATGGATGGTTTGGCTCGACGAAGTCCAATCAGAGATTACCCTGGCATCCCATCTCGTGGATTTAGGGGATTTGGCCCTGATGATTTTGATGGCAGGGATTTCCATCGATTTGGTGATCCGCTTGGCAGGGAATTCCATGAGAATAGGTTTCCTAATATGCACAGTCATTTTCATAGGGGTGAGTTTGAGGGTCCTGGTAATATGCGAATGGATGATCATATGAGGAACGATTTGATTGGTCAAGGTGGTCACCCTGGTCATCTGCGGAGGGGAGATCATTTGGGTCCTCATAACTTGCCTGGTCATTTGCATTTGCGAGAGCATGTGGGTTTTGGTGTCCATCCTCGACATGCAGGGCCTGGGAGCTTTGAATCATTTATTGGCAACAGGGCAAGTCATCCACGTCTTGGTGAGCCTGGATTTAGGAGCAGCTTTTCACTTCAGAGATTTCCAAATGATGGAACTTATACAGGAGAGCTAGAGTCTTTTGATCACTCAAGGAAAAGGAAACCAGCCAGCATGGGATGGTGTCGTATCTGTAAAGTTGACTGTGAAACAGTTGAGGGCTTGGACGTGCATTCACAAACAAGGGAGCACCAGAGAATGGCTATGGAAATGGTACAAAGTATCAAGCAGAATGCCAAAAAGCAGAAATTAACCTCTGGTGATCAGTCCTCGGTAGAGGATGCAAACAAGTCAAAGATTTCCAGTTTTGAGAGCCGGGTTGAAAAGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000793 GO:0000794 GO:0001701 GO:0001756 GO:0001932 GO:0002020 GO:0002791 GO:0002793 GO:0003002 GO:0003007 GO:0003008 GO:0003012 GO:0003013 GO:0003015 GO:0003300 GO:0003674 GO:0003779 GO:0003824 GO:0004672 GO:0004674 GO:0004713 GO:0005198 GO:0005200 GO:0005488 GO:0005509 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005856 GO:0005859 GO:0005865 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006936 GO:0006941 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0007399 GO:0007417 GO:0007420 GO:0007507 GO:0007512 GO:0007517 GO:0008015 GO:0008092 GO:0008150 GO:0008152 GO:0008307 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009653 GO:0009790 GO:0009792 GO:0009887 GO:0009888 GO:0009893 GO:0009952 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010604 GO:0010628 GO:0010737 GO:0010927 GO:0014706 GO:0014866 GO:0014896 GO:0014897 GO:0015629 GO:0016043 GO:0016301 GO:0016310 GO:0016459 GO:0016460 GO:0016740 GO:0016772 GO:0016773 GO:0018108 GO:0018193 GO:0018212 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0021591 GO:0022607 GO:0023052 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030036 GO:0030154 GO:0030239 GO:0030240 GO:0030241 GO:0030506 GO:0031032 GO:0031033 GO:0031034 GO:0031323 GO:0031399 GO:0031430 GO:0031433 GO:0031672 GO:0031674 GO:0031974 GO:0031981 GO:0032268 GO:0032501 GO:0032502 GO:0032879 GO:0032880 GO:0032989 GO:0032991 GO:0033058 GO:0034622 GO:0035051 GO:0035265 GO:0035282 GO:0035556 GO:0035994 GO:0035995 GO:0036211 GO:0036379 GO:0040007 GO:0040011 GO:0042221 GO:0042325 GO:0042692 GO:0042802 GO:0042805 GO:0043009 GO:0043056 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043292 GO:0043412 GO:0043549 GO:0043621 GO:0043933 GO:0044057 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044449 GO:0044464 GO:0044877 GO:0045214 GO:0045859 GO:0046872 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048589 GO:0048644 GO:0048646 GO:0048729 GO:0048731 GO:0048738 GO:0048739 GO:0048747 GO:0048769 GO:0048856 GO:0048869 GO:0050708 GO:0050714 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0050982 GO:0051015 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051146 GO:0051171 GO:0051174 GO:0051222 GO:0051223 GO:0051239 GO:0051246 GO:0051338 GO:0051371 GO:0051393 GO:0051592 GO:0051606 GO:0051716 GO:0055001 GO:0055002 GO:0055003 GO:0055006 GO:0055007 GO:0055008 GO:0055013 GO:0060047 GO:0060048 GO:0060255 GO:0060322 GO:0060415 GO:0060419 GO:0060429 GO:0060537 GO:0061053 GO:0061061 GO:0065003 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070925 GO:0071688 GO:0071704 GO:0071840 GO:0072359 GO:0080090 GO:0090087 GO:0090257 GO:0097435 GO:0097493 GO:0099080 GO:0099081 GO:0099512 GO:0140096 GO:1901077 GO:1901564 GO:1901897 GO:1903530 GO:1903532 GO:1904951
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1311

Amino Acids

142.99

Weight (kDa)

7.02

Isoelectric Point (pI)

60.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2628
AarI CACCTGC 1 cut(s) 1276
AatII GACGTC 1 cut(s) 536
Acc16I TGCGCA 1 cut(s) 933
Acc36I ACCTGC 3 cut(s) 819, 1276, 1912
AccB1I GGYRCC 3 cut(s) 988, 2309, 2659
AccB7I CCANNNNNTGG 9 cut(s) 176, 779, 1721, 2678, 3182, 3254, 3605, 3656, 3802
AccBSI CCGCTC 1 cut(s) 823
AccI GTMKAC 1 cut(s) 81
AccIII TCCGGA 1 cut(s) 2068
AciI CCGC 8 cut(s) 44, 800, 821, 1872, 2373, 2767, 3317, 3463
AclI AACGTT 1 cut(s) 1434
AclWI GGATC 4 cut(s) 683, 2289, 3086, 3308
AcoI YGGCCR 3 cut(s) 2721, 2740, 2768
AcsI RAATTY 8 cut(s) 346, 589, 1002, 1485, 1852, 1924, 3085, 3329
AcuI CTGAAG 7 cut(s) 453, 959, 2046, 2211, 2328, 2439, 3623
AcyI GRCGYC 1 cut(s) 533
AfaI GTAC 8 cut(s) 53, 665, 716, 1279, 1728, 2146, 2364, 3819
AflIII ACRYGT 1 cut(s) 2524
AjiI CACGTC 2 cut(s) 3602, 3772
AjuI GAANNNNNNNTTGG 6 cut(s) 882, 914, 1765, 1797, 3561, 3593
AleI CACNNNNGTG 1 cut(s) 279
AloI GAACNNNNNNTCC 2 cut(s) 1476, 1508
Alw21I GWGCWC 8 cut(s) 113, 155, 234, 290, 1257, 2431, 2686, 3795
Alw26I GTCTC 7 cut(s) 540, 1155, 1644, 1665, 2919, 3046, 3129
Alw44I GTGCAC 3 cut(s) 109, 286, 2427
AlwI GGATC 4 cut(s) 683, 2289, 3086, 3308
AlwNI CAGNNNCTG 5 cut(s) 197, 476, 760, 3095, 3557
Ama87I CYCGRG 1 cut(s) 2864
Aor13HI TCCGGA 1 cut(s) 2068
ApaLI GTGCAC 3 cut(s) 109, 286, 2427
ApoI RAATTY 8 cut(s) 346, 589, 1002, 1485, 1852, 1924, 3085, 3329
ArsI GACNNNNNNTTYG 2 cut(s) 2087, 2119
AseI ATTAAT 2 cut(s) 2943, 3146
Asp700I GAANNNNTTC 1 cut(s) 2915
AspA2I CCTAGG 2 cut(s) 2570, 2981
AspLEI GCGC 4 cut(s) 934, 1503, 2644, 3195
AspS9I GGNCC 9 cut(s) 299, 2459, 2516, 2621, 2669, 3272, 3389, 3482, 3554
AsuC2I CCSGG 5 cut(s) 62, 2478, 2865, 2866, 3920
AsuII TTCGAA 2 cut(s) 587, 2847
AvaI CYCGRG 1 cut(s) 2864
AvaII GGWCC 4 cut(s) 2516, 2669, 3389, 3482
AvrII CCTAGG 2 cut(s) 2570, 2981
AxyI CCTNAGG 1 cut(s) 2511
BaeGI GKGCMC 5 cut(s) 113, 290, 1308, 2431, 2662
BaeI ACNNNNGTAYC 4 cut(s) 689, 722, 1760, 1793
BalI TGGCCA 2 cut(s) 2723, 2742
BanI GGYRCC 3 cut(s) 988, 2309, 2659
BarI GAAGNNNNNNTAC 2 cut(s) 1884, 1916
BauI CACGAG 2 cut(s) 2934, 3252
BbsI GAAGAC 1 cut(s) 65
Bbv12I GWGCWC 8 cut(s) 113, 155, 234, 290, 1257, 2431, 2686, 3795
BbvCI CCTCAGC 3 cut(s) 1353, 1536, 1734
BceAI ACGGC 1 cut(s) 1718
BcgI CGANNNNNNTGC 2 cut(s) 2418, 2452
BciVI GTATCC 2 cut(s) 687, 1788
BclI TGATCA 3 cut(s) 3412, 3688, 3865
BcnI CCSGG 5 cut(s) 62, 2478, 2865, 2866, 3920
BcoDI GTCTC 7 cut(s) 540, 1155, 1644, 1665, 2919, 3046, 3129
BfaI CTAG 7 cut(s) 2076, 2304, 2571, 2691, 2982, 3036, 3677
BfmI CTRYAG 5 cut(s) 354, 761, 1167, 1944, 3096
BfoI RGCGCY 2 cut(s) 1504, 3196
BfuAI ACCTGC 3 cut(s) 819, 1276, 1912
BfuI GTATCC 2 cut(s) 687, 1788
BlnI CCTAGG 2 cut(s) 2570, 2981
BmcAI AGTACT 5 cut(s) 53, 665, 716, 1728, 2146
Bme18I GGWCC 4 cut(s) 2516, 2669, 3389, 3482
BmeT110I CYCGRG 1 cut(s) 2864
BmgBI CACGTC 2 cut(s) 3602, 3772
BmgT120I GGNCC 9 cut(s) 299, 2459, 2516, 2621, 2669, 3272, 3389, 3482, 3554
BmrI ACTGGG 1 cut(s) 1853
BmsI GCATC 8 cut(s) 190, 397, 1701, 2290, 2370, 2603, 3252, 3874
BmuI ACTGGG 1 cut(s) 1853
BoxI GACNNNNGTC 2 cut(s) 143, 953
BpiI GAAGAC 1 cut(s) 65
BpmI CTGGAG 4 cut(s) 597, 1440, 1481, 2396
Bpu10I CCTNAGC 3 cut(s) 1353, 1536, 1734
Bpu14I TTCGAA 2 cut(s) 587, 2847
BpuEI CTTGAG 6 cut(s) 585, 861, 1038, 1281, 2954, 3679
BpuMI CCSGG 5 cut(s) 62, 2478, 2865, 2866, 3920
Bsa29I ATCGAT 2 cut(s) 2947, 3304
BsaBI GATNNNNATC 1 cut(s) 3300
BsaHI GRCGYC 1 cut(s) 533
BsaI GGTCTC 2 cut(s) 2919, 3046
BsaWI WCCGGW 1 cut(s) 2068
BsaXI ACNNNNNCTCC 4 cut(s) 532, 562, 807, 837
Bse118I RCCGGY 2 cut(s) 1879, 2770
Bse21I CCTNAGG 1 cut(s) 2511
Bse3DI GCAATG 3 cut(s) 95, 262, 1515
Bse8I GATNNNNATC 1 cut(s) 3300
BseAI TCCGGA 1 cut(s) 2068
BseCI ATCGAT 2 cut(s) 2947, 3304
BseJI GATNNNNATC 1 cut(s) 3300
BseMI GCAATG 3 cut(s) 95, 262, 1515
BseRI GAGGAG 4 cut(s) 1041, 2330, 2637, 3022
BseSI GKGCMC 5 cut(s) 113, 290, 1308, 2431, 2662
BseX3I CGGCCG 1 cut(s) 2768
BseYI CCCAGC 2 cut(s) 1119, 2210
BsgI GTGCAG 3 cut(s) 419, 449, 1428
Bsh1285I CGRYCG 1 cut(s) 2771
BshNI GGYRCC 3 cut(s) 988, 2309, 2659
BshVI ATCGAT 2 cut(s) 2947, 3304
BsiEI CGRYCG 1 cut(s) 2771
BsiHKAI GWGCWC 8 cut(s) 113, 155, 234, 290, 1257, 2431, 2686, 3795
BsiHKCI CYCGRG 1 cut(s) 2864
BsiSI CCGG 8 cut(s) 62, 1880, 2069, 2477, 2771, 2865, 2897, 3919
BslFI GGGAC 6 cut(s) 796, 1102, 1162, 1572, 1713, 2529
BsmAI GTCTC 7 cut(s) 540, 1155, 1644, 1665, 2919, 3046, 3129
BsmBI CGTCTC 2 cut(s) 540, 1155
BsmFI GGGAC 6 cut(s) 796, 1102, 1162, 1572, 1713, 2529
BsmI GAATGC 3 cut(s) 1168, 3775, 3841
Bso31I GGTCTC 2 cut(s) 2919, 3046
BsoBI CYCGRG 1 cut(s) 2864
Bsp119I TTCGAA 2 cut(s) 587, 2847
Bsp13I TCCGGA 1 cut(s) 2068
Bsp143I GATC 9 cut(s) 688, 2281, 2965, 3091, 3313, 3412, 3472, 3688, 3865
Bsp19I CCATGG 1 cut(s) 2737
BspACI CCGC 8 cut(s) 44, 800, 821, 1872, 2373, 2767, 3317, 3463
BspDI ATCGAT 2 cut(s) 2947, 3304
BspEI TCCGGA 1 cut(s) 2068
BspMAI CTGCAG 2 cut(s) 765, 3100
BspMI ACCTGC 3 cut(s) 819, 1276, 1912
BspPI GGATC 4 cut(s) 683, 2289, 3086, 3308
BspT104I TTCGAA 2 cut(s) 587, 2847
BspT107I GGYRCC 3 cut(s) 988, 2309, 2659
BspTNI GGTCTC 2 cut(s) 2919, 3046
BsrBI CCGCTC 1 cut(s) 823
BsrDI GCAATG 3 cut(s) 95, 262, 1515
BsrFI RCCGGY 2 cut(s) 1879, 2770
BssAI RCCGGY 2 cut(s) 1879, 2770
BssMI GATC 9 cut(s) 688, 2281, 2965, 3091, 3313, 3412, 3472, 3688, 3865
BssNI GRCGYC 1 cut(s) 533
BssSI CACGAG 2 cut(s) 2934, 3252
BssT1I CCWWGG 4 cut(s) 2570, 2737, 2981, 3113
Bst2BI CACGAG 2 cut(s) 2934, 3252
Bst6I CTCTTC 3 cut(s) 2094, 2124, 3015
BstACI GRCGYC 1 cut(s) 533
BstAPI GCANNNNNTGC 2 cut(s) 760, 2600
BstBI TTCGAA 2 cut(s) 587, 2847
BstDSI CCRYGG 1 cut(s) 2737
BstEII GGTNACC 2 cut(s) 3170, 3446
BstENI CCTNNNNNAGG 3 cut(s) 2454, 2652, 3046
BstH2I RGCGCY 2 cut(s) 1504, 3196
BstHHI GCGC 4 cut(s) 934, 1503, 2644, 3195
BstKTI GATC 9 cut(s) 691, 2284, 2968, 3094, 3316, 3415, 3475, 3691, 3868
BstMAI GTCTC 7 cut(s) 540, 1155, 1644, 1665, 2919, 3046, 3129
BstMBI GATC 9 cut(s) 688, 2281, 2965, 3091, 3313, 3412, 3472, 3688, 3865
BstMCI CGRYCG 1 cut(s) 2771
BstNSI RCATGY 5 cut(s) 1672, 2528, 2821, 3524, 3551
BstPAI GACNNNNGTC 2 cut(s) 143, 953
BstPI GGTNACC 2 cut(s) 3170, 3446
BstSFI CTRYAG 5 cut(s) 354, 761, 1167, 1944, 3096
BstSLI GKGCMC 5 cut(s) 113, 290, 1308, 2431, 2662
BstV2I GAAGAC 1 cut(s) 65
BstX2I RGATCY 1 cut(s) 3091
BstXI CCANNNNNNTGG 1 cut(s) 3308
BstYI RGATCY 1 cut(s) 3091
BstZI CGGCCG 1 cut(s) 2768
Bsu15I ATCGAT 2 cut(s) 2947, 3304
Bsu36I CCTNAGG 1 cut(s) 2511
BsuI GTATCC 2 cut(s) 687, 1788
BsuTUI ATCGAT 2 cut(s) 2947, 3304
BtgI CCRYGG 1 cut(s) 2737
BtrI CACGTC 2 cut(s) 3602, 3772
BtsI GCAGTG 1 cut(s) 2221
BtsIMutI CAGTG 5 cut(s) 113, 534, 644, 1257, 2221
BveI ACCTGC 3 cut(s) 819, 1276, 1912
CaiI CAGNNNCTG 5 cut(s) 197, 476, 760, 3095, 3557
CfoI GCGC 4 cut(s) 934, 1503, 2644, 3195
Cfr10I RCCGGY 2 cut(s) 1879, 2770
Cfr13I GGNCC 9 cut(s) 299, 2459, 2516, 2621, 2669, 3272, 3389, 3482, 3554
Cfr9I CCCGGG 1 cut(s) 2864
ClaI ATCGAT 2 cut(s) 2947, 3304
Csp6I GTAC 8 cut(s) 52, 664, 715, 1278, 1727, 2145, 2363, 3818
CspCI CAANNNNNGTGG 4 cut(s) 352, 387, 1677, 1712
CviQI GTAC 8 cut(s) 52, 664, 715, 1278, 1727, 2145, 2363, 3818
DpnI GATC 9 cut(s) 690, 2283, 2967, 3093, 3315, 3414, 3474, 3690, 3867
DpnII GATC 9 cut(s) 688, 2281, 2965, 3091, 3313, 3412, 3472, 3688, 3865
EaeI YGGCCR 3 cut(s) 2721, 2740, 2768
EagI CGGCCG 1 cut(s) 2768
Eam1104I CTCTTC 3 cut(s) 2094, 2124, 3015
EarI CTCTTC 3 cut(s) 2094, 2124, 3015
EclXI CGGCCG 1 cut(s) 2768
Eco130I CCWWGG 4 cut(s) 2570, 2737, 2981, 3113
Eco147I AGGCCT 2 cut(s) 2509, 2986
Eco31I GGTCTC 2 cut(s) 2919, 3046
Eco47I GGWCC 4 cut(s) 2516, 2669, 3389, 3482
Eco52I CGGCCG 1 cut(s) 2768
Eco57I CTGAAG 7 cut(s) 453, 959, 2046, 2211, 2328, 2439, 3623
Eco81I CCTNAGG 1 cut(s) 2511
Eco88I CYCGRG 1 cut(s) 2864
Eco91I GGTNACC 2 cut(s) 3170, 3446
EcoNI CCTNNNNNAGG 3 cut(s) 2454, 2652, 3046
EcoO109I RGGNCCY 6 cut(s) 299, 2459, 2516, 3389, 3482, 3554
EcoO65I GGTNACC 2 cut(s) 3170, 3446
EcoRI GAATTC 3 cut(s) 589, 1485, 3329
EcoT14I CCWWGG 4 cut(s) 2570, 2737, 2981, 3113
ErhI CCWWGG 4 cut(s) 2570, 2737, 2981, 3113
Esp3I CGTCTC 2 cut(s) 540, 1155
FalI AAGNNNNNCTT 2 cut(s) 2250, 2282
FaqI GGGAC 6 cut(s) 796, 1102, 1162, 1572, 1713, 2529
FauI CCCGC 1 cut(s) 37
FauNDI CATATG 2 cut(s) 1741, 3417
FbaI TGATCA 3 cut(s) 3412, 3688, 3865
FblI GTMKAC 1 cut(s) 81
FspBI CTAG 7 cut(s) 2076, 2304, 2571, 2691, 2982, 3036, 3677
FspI TGCGCA 1 cut(s) 933
GlaI GCGC 4 cut(s) 933, 1502, 2643, 3194
GsaI CCCAGC 2 cut(s) 1123, 2214
GsuI CTGGAG 4 cut(s) 597, 1440, 1481, 2396
HaeII RGCGCY 2 cut(s) 1504, 3196
HapII CCGG 8 cut(s) 62, 1880, 2069, 2477, 2771, 2865, 2897, 3919
HhaI GCGC 4 cut(s) 934, 1503, 2644, 3195
Hin1I GRCGYC 1 cut(s) 533
Hin6I GCGC 4 cut(s) 932, 1501, 2642, 3193
HinP1I GCGC 4 cut(s) 932, 1501, 2642, 3193
HincII GTYRAC 1 cut(s) 3745
HindII GTYRAC 1 cut(s) 3745
HpaII CCGG 8 cut(s) 62, 1880, 2069, 2477, 2771, 2865, 2897, 3919
Hpy166II GTNNAC 9 cut(s) 82, 111, 288, 1462, 2180, 2429, 2672, 3188, 3745
Hpy188III TCNNGA 8 cut(s) 148, 461, 1482, 1857, 2069, 2484, 2996, 3036
Hpy8I GTNNAC 9 cut(s) 82, 111, 288, 1462, 2180, 2429, 2672, 3188, 3745
Hpy99I CGWCG 2 cut(s) 149, 3219
HpyCH4IV ACGT 7 cut(s) 144, 533, 1276, 1434, 1585, 3601, 3771
HpySE526I ACGT 7 cut(s) 144, 533, 1276, 1434, 1585, 3601, 3771
Hsp92I GRCGYC 1 cut(s) 533
HspAI GCGC 4 cut(s) 932, 1501, 2642, 3193
Kpn2I TCCGGA 1 cut(s) 2068
KroI GCCGGC 2 cut(s) 1879, 2770
KroNI GCCGGC 2 cut(s) 1881, 2772
Ksp22I TGATCA 3 cut(s) 3412, 3688, 3865
Kzo9I GATC 9 cut(s) 688, 2281, 2965, 3091, 3313, 3412, 3472, 3688, 3865
LweI GCATC 8 cut(s) 190, 397, 1701, 2290, 2370, 2603, 3252, 3874
MaeI CTAG 7 cut(s) 2076, 2304, 2571, 2691, 2982, 3036, 3677
MaeII ACGT 7 cut(s) 144, 533, 1276, 1434, 1585, 3601, 3771
MaeIII GTNAC 9 cut(s) 102, 187, 523, 529, 1899, 2439, 3026, 3170, 3446
MalI GATC 9 cut(s) 690, 2283, 2967, 3093, 3315, 3414, 3474, 3690, 3867
MbiI CCGCTC 1 cut(s) 823
MboI GATC 9 cut(s) 688, 2281, 2965, 3091, 3313, 3412, 3472, 3688, 3865
MflI RGATCY 1 cut(s) 3091
MlsI TGGCCA 2 cut(s) 2723, 2742
MluNI TGGCCA 2 cut(s) 2723, 2742
MlyI GAGTC 5 cut(s) 1330, 2081, 2111, 3072, 3689
MmeI TCCRAC 3 cut(s) 650, 1546, 1987
Mox20I TGGCCA 2 cut(s) 2723, 2742
MroI TCCGGA 1 cut(s) 2068
MroNI GCCGGC 2 cut(s) 1879, 2770
MroXI GAANNNNTTC 1 cut(s) 2915
MscI TGGCCA 2 cut(s) 2723, 2742
MseI TTAA 6 cut(s) 1965, 2495, 2886, 2943, 3146, 3854
MslI CAYNNNNRTG 5 cut(s) 279, 1623, 2742, 3156, 3375
Msp20I TGGCCA 2 cut(s) 2723, 2742
MspA1I CMGCKG 3 cut(s) 485, 2767, 3101
MspI CCGG 8 cut(s) 62, 1880, 2069, 2477, 2771, 2865, 2897, 3919
Mva1269I GAATGC 3 cut(s) 1168, 3775, 3841
NaeI GCCGGC 2 cut(s) 1881, 2772
NciI CCSGG 5 cut(s) 62, 2478, 2865, 2866, 3920
NcoI CCATGG 1 cut(s) 2737
NdeI CATATG 2 cut(s) 1741, 3417
NdeII GATC 9 cut(s) 688, 2281, 2965, 3091, 3313, 3412, 3472, 3688, 3865
NgoMIV GCCGGC 2 cut(s) 1879, 2770
NmuCI GTSAC 5 cut(s) 102, 187, 529, 3170, 3446
NsbI TGCGCA 1 cut(s) 933
NspI RCATGY 5 cut(s) 1672, 2528, 2821, 3524, 3551
NspV TTCGAA 2 cut(s) 587, 2847
OliI CACNNNNGTG 1 cut(s) 279
PaeI GCATGC 1 cut(s) 1672
PaqCI CACCTGC 1 cut(s) 1276
PasI CCCWGGG 1 cut(s) 1591
PceI AGGCCT 2 cut(s) 2509, 2986
PciI ACATGT 1 cut(s) 2524
PctI GAATGC 3 cut(s) 1168, 3775, 3841
PdiI GCCGGC 2 cut(s) 1881, 2772
PdmI GAANNNNTTC 1 cut(s) 2915
PflFI GACNNNGTC 1 cut(s) 3219
PflMI CCANNNNNTGG 9 cut(s) 176, 779, 1721, 2678, 3182, 3254, 3605, 3656, 3802
PfoI TCCNGGA 1 cut(s) 679
PleI GAGTC 5 cut(s) 1330, 2080, 2110, 3071, 3688
PpsI GAGTC 5 cut(s) 1330, 2080, 2110, 3071, 3688
PpuMI RGGWCCY 3 cut(s) 2516, 3389, 3482
PscI ACATGT 1 cut(s) 2524
PshAI GACNNNNGTC 2 cut(s) 143, 953
PshBI ATTAAT 2 cut(s) 2943, 3146
PsiI TTATAA 1 cut(s) 2628
Psp1406I AACGTT 1 cut(s) 1434
Psp5II RGGWCCY 3 cut(s) 2516, 3389, 3482
PspEI GGTNACC 2 cut(s) 3170, 3446
PspFI CCCAGC 2 cut(s) 1119, 2210
PspPI GGNCC 9 cut(s) 299, 2459, 2516, 2621, 2669, 3272, 3389, 3482, 3554
PspPPI RGGWCCY 3 cut(s) 2516, 3389, 3482
PstI CTGCAG 2 cut(s) 765, 3100
PstNI CAGNNNCTG 5 cut(s) 197, 476, 760, 3095, 3557
PsuI RGATCY 1 cut(s) 3091
PsyI GACNNNGTC 1 cut(s) 3219
PvuII CAGCTG 2 cut(s) 485, 3101
RsaI GTAC 8 cut(s) 53, 665, 716, 1279, 1728, 2146, 2364, 3819
RsaNI GTAC 8 cut(s) 52, 664, 715, 1278, 1727, 2145, 2363, 3818
RseI CAYNNNNRTG 5 cut(s) 279, 1623, 2742, 3156, 3375
SaqAI TTAA 6 cut(s) 1965, 2495, 2886, 2943, 3146, 3854
Sau3AI GATC 9 cut(s) 688, 2281, 2965, 3091, 3313, 3412, 3472, 3688, 3865
Sau96I GGNCC 9 cut(s) 299, 2459, 2516, 2621, 2669, 3272, 3389, 3482, 3554
ScaI AGTACT 5 cut(s) 53, 665, 716, 1728, 2146
SchI GAGTC 5 cut(s) 1330, 2081, 2111, 3072, 3689
SfaNI GCATC 8 cut(s) 190, 397, 1701, 2290, 2370, 2603, 3252, 3874
SfcI CTRYAG 5 cut(s) 354, 761, 1167, 1944, 3096
SfuI TTCGAA 2 cut(s) 587, 2847
SinI GGWCC 4 cut(s) 2516, 2669, 3389, 3482
SmaI CCCGGG 1 cut(s) 2866
SmiMI CAYNNNNRTG 5 cut(s) 279, 1623, 2742, 3156, 3375
SmlI CTYRAG 6 cut(s) 600, 876, 1053, 1296, 2969, 3694
SmoI CTYRAG 6 cut(s) 600, 876, 1053, 1296, 2969, 3694
SphI GCATGC 1 cut(s) 1672
SseBI AGGCCT 2 cut(s) 2509, 2986
SsiI CCGC 8 cut(s) 44, 800, 821, 1872, 2373, 2767, 3317, 3463
SspI AATATT 1 cut(s) 1544
SspMI CTAG 7 cut(s) 2076, 2304, 2571, 2691, 2982, 3036, 3677
StuI AGGCCT 2 cut(s) 2509, 2986
StyI CCWWGG 4 cut(s) 2570, 2737, 2981, 3113
TaiI ACGT 7 cut(s) 147, 536, 1279, 1437, 1588, 3604, 3774
TaqI TCGA 9 cut(s) 587, 1208, 1631, 2847, 2947, 3045, 3214, 3304, 3544
TatI WGTACW 6 cut(s) 51, 663, 714, 1726, 2144, 2362
TauI GCSGC 2 cut(s) 2376, 2770
Tru1I TTAA 6 cut(s) 1965, 2495, 2886, 2943, 3146, 3854
Tru9I TTAA 6 cut(s) 1965, 2495, 2886, 2943, 3146, 3854
TscAI CASTG 5 cut(s) 113, 534, 644, 1257, 2221
TseFI GTSAC 5 cut(s) 102, 187, 529, 3170, 3446
Tsp45I GTSAC 5 cut(s) 102, 187, 529, 3170, 3446
TspMI CCCGGG 1 cut(s) 2864
TspRI CASTG 5 cut(s) 113, 534, 644, 1257, 2221
Tth111I GACNNNGTC 1 cut(s) 3219
Van91I CCANNNNNTGG 9 cut(s) 176, 779, 1721, 2678, 3182, 3254, 3605, 3656, 3802
VneI GTGCAC 3 cut(s) 109, 286, 2427
VpaK11BI GGWCC 4 cut(s) 2516, 2669, 3389, 3482
VspI ATTAAT 2 cut(s) 2943, 3146
XagI CCTNNNNNAGG 3 cut(s) 2454, 2652, 3046
XapI RAATTY 8 cut(s) 346, 589, 1002, 1485, 1852, 1924, 3085, 3329
XbaI TCTAGA 1 cut(s) 3035
XceI RCATGY 5 cut(s) 1672, 2528, 2821, 3524, 3551
XcmI CCANNNNNNNNNTGG 1 cut(s) 677
XmaI CCCGGG 1 cut(s) 2864
XmaJI CCTAGG 2 cut(s) 2570, 2981
XmiI GTMKAC 1 cut(s) 81
XmnI GAANNNNTTC 1 cut(s) 2915
XspI CTAG 7 cut(s) 2076, 2304, 2571, 2691, 2982, 3036, 3677
ZraI GACGTC 1 cut(s) 534
ZrmI AGTACT 5 cut(s) 53, 665, 716, 1728, 2146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.