Rmu_ssc0000354.1_g000005

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000354.1
OG0002401
Physical Location & Seq
Reverse (-)
19713 .. 24096
4384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000354.1_g000005.1.cds

Sequence Viewer

Length: 3945 bp
atgggatttgataatgagtgcatactgaacatccaatctcttgcgggagagtacttttgcccggtctgtcgtcttcttgtctacccaaatgaagcattgcaatcacagtgcactcatctatactgtaagccctgtttgacatacgttgtgagcactaccaaagcgtgtccatacgatggctacttagtgacagaagccgatgcgaagcccctcgttgaatcaaataaagtgctcgctgaaaccatagggaaaatagcagttcattgcctgtatcacaggagtgggtgcacatggcaggggcctttatctgattgcacatctcattgttctggttgtgcctttggaaattctcctgtagtctgcaacaggtgtggaatccaaattgtgcatcgtcaagtgcaggaacacgcacaaagttgccctggtgtgcagccccaagcacagcaggcagagggtgttccagacacttcagcctctggcacagctgctactgctaatcagaaccaggctgccactcagacagttacagtgacgtctcagacacaggctccccaaactgcaagtgccactatacctggagctgattcgaattctcaggctcaagctgttgttcaagctgctgctccgactgctgaccagtggtatcagcaacaatatcagcagtactaccagcagtatcctggatatgatccataccaacaacagtatcagcagtactatccttatcagcagccagcaattccacaaccacagcagcctttgcaggctaatccaccctatgggactgttcagcctcaaccgcaggcatacctgcaacctccgctcctacctcaacctcaacctcaacctcaacctcaaccacagacccaaccatctcaatctcaagctcaaccccaaccacaagttcagcccccaattatgcaggctcctatggctgcgcatactcaaaaccagacatcagtcaatcaacagcttcaacttcagcctgctatggcaccacattcacaaattcaggcacaatcttacccatcagctcatgggcaggctcaacttcctcctcaagcatatttccaagtccaaatgcctcaatatcagcagccacaagttcagcagcttacacagtcccagcttcaacagaatccccaacttcacccgtctcagcccatgaatgctacagtccagtcccagacacagctcccatcatctaattcatcgactggcaatcatttgttccctcaatctcaccttcctcaaccagtgctctcagcagcctcccaacaacgtactatgcaggtgcaatctcaaggagtgcccaactctcagtcgcaaaaccatgttcagactcagattcagtttcctcagcaaccccttttgcgaccacccccgtttcaaacaatagttccaaaccagcagcagacagccttgttgccttcacctaacgttatctctgcacaacagcctccagttcactcctttgctcaacaatctggaattcctgttctccagcgccctgcaatgcagccagtccaacagctaaatcctcagcaatatttccacatacaacagacaccccccacattgtcgcaattacgtccccagggccaatctcaatcattcccacaacatattcgtgcctcgaaccagtctcagcagaatgttgtattgtctcagggcgtgcaacatattcagccctcaaatcttgtgggacggcctatgatgccaagtcatggagtactacctcagccatatgcacaaacagtaggaggagttctgccaagaccaatgtatcctgctctaaaccatcaatctacaaatcagaataatataggcagaaccaacaatcaagtgcaactgggagcaaattcaagaccaacaatgaccgcaaggccggcagagaaagaagctgagttacctgctaataatgttgcacagaatttgggtgtattgtctgctgtaggtgctgattccgaggttaaaactgtgaaatctgaggtggatatgaagtccattgatgttggaaataaaccttcttctgaagatagaagttatcagggtaatacatctgccaaagagattccggagtctagggggatgttgggggcgaatggagagtctgaaagtaagccaacattgaaggaagagggtgtaaatagtactttggaggattcaagtaatgataaattgggtgaacttgtagctgaaggtgcaaaagatgttcccagcagtggcatgaaacagggggagcgtaaagaaatgccatcagaagaagcacaacttcatggagtaaaggatcaaaaattgcaaaaagatgctagtggcaccgaggagggttcccaaactgtttctacttcttcagctcctataggtcaagtacaagctggcggcttgatgcaaccttcatatcctggctctgctattctccagcaaaggccaggtgcacctccttcgttacaagtgccttcttcagggcctcaacatcatataccgggttctggacaacccttaattcatgcaaggcctcagggacctggacatgtgcctgggcaggcttctcatctatctgagcattttcaatcacctaggggaaacctgggatttgcagcatcatctgcaagtgcaagtcagcatgggccttataaccaaagccatgcgcctcctcatccaggggcacccagaggtccaccatttggagcaccccctagtgcttttgattttcatgggggtataatggccagagcagcaccccatggccatgaaggtcagaggggtcagcagcggccggcttttcagatggaacagggagcaactggacagccatcctcaaacatgctcagaataaatggaaatccaggcttcgaatccttatcaactcccgggttgagggatgaaagatttaaggctttgccggatgggaggttgaatcccttcccaggagacccaactcgtgtgattaatcgattagggtttgaagatgatctcaagcaattccctaggccttcttatctggattctgagcctcttccaaaattagggagttactcctctagagccttcgataggagacctcttggagtcaattatgatactcgtctaaattcagatcctgcagctggctctgctccaaggttcctgtctccctatggtcatggtggattaattcatgccaatgatacaggtggtcacccagattttggtggacagcgccttatggatggtttggctcgacgaagtccaatcagagattaccctggcatcccatctcgtggatttaggggatttggccctgatgattttgatggcagggatttccatcgatttggtgatccgcttggtagggaattccatgagaataggtttcctaatatgcacagtcattttcataggggtgagtttgagggtcctggtaatatgcgaatggatgatcatacgaggaacgatttgattggtcaaggtggtcaccctggtcatctgcggaggggagatcatttgggtcctcataacttgcctggtcatttgcatttgcgagagcatgtgggttttggtgtccatcctcgacatgcagggcctgggagctttgaatcatttattggcaacagggcaagtcatccacgtcttggcgagcctggatttaggagcagcttttcacttcagagatttccaaatgatggaacttatacaggagagctagagtcttttgatcactcaaggaaaaggaaaccagccagcatgggatggtgtcgtatctgtaaagttgactgtgaaacagttgagggcttggacgtgcattcacaaacaagggagcaccagagaatggctatggaaatggtacaaagtatcaagcagaatgccaaaaagcagaaattaacctctggtgatcagtcctcggtagaggatgcaaacaagtcaaagatttccagttttgagagccgggttgaaaagcattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000793 GO:0000794 GO:0001701 GO:0001756 GO:0001932 GO:0002020 GO:0002791 GO:0002793 GO:0003002 GO:0003007 GO:0003008 GO:0003012 GO:0003013 GO:0003015 GO:0003300 GO:0003674 GO:0003779 GO:0003824 GO:0004672 GO:0004674 GO:0004713 GO:0005198 GO:0005200 GO:0005488 GO:0005509 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005856 GO:0005859 GO:0005865 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006936 GO:0006941 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0007399 GO:0007417 GO:0007420 GO:0007507 GO:0007512 GO:0007517 GO:0008015 GO:0008092 GO:0008150 GO:0008152 GO:0008307 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009653 GO:0009790 GO:0009792 GO:0009887 GO:0009888 GO:0009893 GO:0009952 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010604 GO:0010628 GO:0010737 GO:0010927 GO:0014706 GO:0014866 GO:0014896 GO:0014897 GO:0015629 GO:0016043 GO:0016301 GO:0016310 GO:0016459 GO:0016460 GO:0016740 GO:0016772 GO:0016773 GO:0018108 GO:0018193 GO:0018212 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0021591 GO:0022607 GO:0023052 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030036 GO:0030154 GO:0030239 GO:0030240 GO:0030241 GO:0030506 GO:0031032 GO:0031033 GO:0031034 GO:0031323 GO:0031399 GO:0031430 GO:0031433 GO:0031672 GO:0031674 GO:0031974 GO:0031981 GO:0032268 GO:0032501 GO:0032502 GO:0032879 GO:0032880 GO:0032989 GO:0032991 GO:0033058 GO:0034622 GO:0035051 GO:0035265 GO:0035282 GO:0035556 GO:0035994 GO:0035995 GO:0036211 GO:0036379 GO:0040007 GO:0040011 GO:0042221 GO:0042325 GO:0042692 GO:0042802 GO:0042805 GO:0043009 GO:0043056 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043292 GO:0043412 GO:0043549 GO:0043621 GO:0043933 GO:0044057 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044449 GO:0044464 GO:0044877 GO:0045214 GO:0045859 GO:0046872 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048589 GO:0048644 GO:0048646 GO:0048729 GO:0048731 GO:0048738 GO:0048739 GO:0048747 GO:0048769 GO:0048856 GO:0048869 GO:0050708 GO:0050714 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0050982 GO:0051015 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051146 GO:0051171 GO:0051174 GO:0051222 GO:0051223 GO:0051239 GO:0051246 GO:0051338 GO:0051371 GO:0051393 GO:0051592 GO:0051606 GO:0051716 GO:0055001 GO:0055002 GO:0055003 GO:0055006 GO:0055007 GO:0055008 GO:0055013 GO:0060047 GO:0060048 GO:0060255 GO:0060322 GO:0060415 GO:0060419 GO:0060429 GO:0060537 GO:0061053 GO:0061061 GO:0065003 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070925 GO:0071688 GO:0071704 GO:0071840 GO:0072359 GO:0080090 GO:0090087 GO:0090257 GO:0097435 GO:0097493 GO:0099080 GO:0099081 GO:0099512 GO:0140096 GO:1901077 GO:1901564 GO:1901897 GO:1903530 GO:1903532 GO:1904951
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1314

Amino Acids

143.1

Weight (kDa)

7.23

Isoelectric Point (pI)

61.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2637
AarI CACCTGC 1 cut(s) 1282
AatII GACGTC 1 cut(s) 536
Acc16I TGCGCA 1 cut(s) 939
Acc36I ACCTGC 3 cut(s) 819, 1282, 1921
AccB1I GGYRCC 3 cut(s) 994, 2318, 2668
AccB7I CCANNNNNTGG 9 cut(s) 176, 779, 1727, 2687, 3191, 3263, 3614, 3665, 3811
AccBSI CCGCTC 1 cut(s) 823
AccI GTMKAC 1 cut(s) 81
AccIII TCCGGA 1 cut(s) 2077
AciI CCGC 8 cut(s) 44, 800, 821, 1881, 2382, 2776, 3326, 3472
AclI AACGTT 1 cut(s) 1440
AclWI GGATC 4 cut(s) 683, 2298, 3095, 3317
AcoI YGGCCR 3 cut(s) 2730, 2749, 2777
AcsI RAATTY 8 cut(s) 346, 589, 1008, 1491, 1861, 1933, 3094, 3338
AcuI CTGAAG 7 cut(s) 453, 965, 2055, 2220, 2337, 2448, 3632
AcyI GRCGYC 1 cut(s) 533
AfaI GTAC 8 cut(s) 53, 665, 716, 1285, 1734, 2155, 2373, 3828
AflIII ACRYGT 1 cut(s) 2533
AjiI CACGTC 2 cut(s) 3611, 3781
AjuI GAANNNNNNNTTGG 4 cut(s) 888, 920, 3570, 3602
AleI CACNNNNGTG 1 cut(s) 279
AloI GAACNNNNNNTCC 2 cut(s) 1482, 1514
Alw21I GWGCWC 8 cut(s) 113, 155, 234, 290, 1263, 2440, 2695, 3804
Alw26I GTCTC 7 cut(s) 540, 1161, 1650, 1671, 2928, 3055, 3138
Alw44I GTGCAC 3 cut(s) 109, 286, 2436
AlwI GGATC 4 cut(s) 683, 2298, 3095, 3317
AlwNI CAGNNNCTG 3 cut(s) 476, 3104, 3566
Ama87I CYCGRG 1 cut(s) 2873
Aor13HI TCCGGA 1 cut(s) 2077
ApaLI GTGCAC 3 cut(s) 109, 286, 2436
ApoI RAATTY 8 cut(s) 346, 589, 1008, 1491, 1861, 1933, 3094, 3338
ArsI GACNNNNNNTTYG 2 cut(s) 2096, 2128
AseI ATTAAT 2 cut(s) 2952, 3155
Asp700I GAANNNNTTC 1 cut(s) 2924
AspA2I CCTAGG 2 cut(s) 2579, 2990
AspLEI GCGC 4 cut(s) 940, 1509, 2653, 3204
AsuC2I CCSGG 5 cut(s) 62, 2487, 2874, 2875, 3929
AsuII TTCGAA 2 cut(s) 587, 2856
AvaI CYCGRG 1 cut(s) 2873
AvaII GGWCC 4 cut(s) 2525, 2678, 3398, 3491
AvrII CCTAGG 2 cut(s) 2579, 2990
AxyI CCTNAGG 1 cut(s) 2520
BaeGI GKGCMC 5 cut(s) 113, 290, 1314, 2440, 2671
BaeI ACNNNNGTAYC 4 cut(s) 689, 722, 1769, 1802
BalI TGGCCA 2 cut(s) 2732, 2751
BanI GGYRCC 3 cut(s) 994, 2318, 2668
BarI GAAGNNNNNNTAC 2 cut(s) 1893, 1925
BauI CACGAG 2 cut(s) 2943, 3261
BbsI GAAGAC 1 cut(s) 65
Bbv12I GWGCWC 8 cut(s) 113, 155, 234, 290, 1263, 2440, 2695, 3804
BbvCI CCTCAGC 3 cut(s) 1359, 1542, 1740
BceAI ACGGC 1 cut(s) 1724
BcgI CGANNNNNNTGC 2 cut(s) 2427, 2461
BciVI GTATCC 2 cut(s) 687, 1797
BclI TGATCA 3 cut(s) 3421, 3697, 3874
BcnI CCSGG 5 cut(s) 62, 2487, 2874, 2875, 3929
BcoDI GTCTC 7 cut(s) 540, 1161, 1650, 1671, 2928, 3055, 3138
BfaI CTAG 7 cut(s) 2085, 2313, 2580, 2700, 2991, 3045, 3686
BfmI CTRYAG 5 cut(s) 354, 1173, 1953, 2361, 3105
BfoI RGCGCY 2 cut(s) 1510, 3205
BfuAI ACCTGC 3 cut(s) 819, 1282, 1921
BfuI GTATCC 2 cut(s) 687, 1797
BlnI CCTAGG 2 cut(s) 2579, 2990
BmcAI AGTACT 5 cut(s) 53, 665, 716, 1734, 2155
Bme18I GGWCC 4 cut(s) 2525, 2678, 3398, 3491
BmeT110I CYCGRG 1 cut(s) 2873
BmgBI CACGTC 2 cut(s) 3611, 3781
BmrI ACTGGG 1 cut(s) 1862
BmsI GCATC 8 cut(s) 190, 397, 1707, 2299, 2379, 2612, 3261, 3883
BmuI ACTGGG 1 cut(s) 1862
BoxI GACNNNNGTC 1 cut(s) 959
BpiI GAAGAC 1 cut(s) 65
BpmI CTGGAG 4 cut(s) 597, 1446, 1487, 2405
Bpu10I CCTNAGC 3 cut(s) 1359, 1542, 1740
Bpu14I TTCGAA 2 cut(s) 587, 2856
BpuEI CTTGAG 6 cut(s) 585, 867, 1044, 1287, 2963, 3688
BpuMI CCSGG 5 cut(s) 62, 2487, 2874, 2875, 3929
Bsa29I ATCGAT 2 cut(s) 2956, 3313
BsaBI GATNNNNATC 1 cut(s) 3309
BsaHI GRCGYC 1 cut(s) 533
BsaI GGTCTC 2 cut(s) 2928, 3055
BsaWI WCCGGW 1 cut(s) 2077
BsaXI ACNNNNNCTCC 4 cut(s) 532, 562, 807, 837
Bse118I RCCGGY 2 cut(s) 1888, 2779
Bse21I CCTNAGG 1 cut(s) 2520
Bse3DI GCAATG 3 cut(s) 95, 262, 1521
Bse8I GATNNNNATC 1 cut(s) 3309
BseAI TCCGGA 1 cut(s) 2077
BseCI ATCGAT 2 cut(s) 2956, 3313
BseJI GATNNNNATC 1 cut(s) 3309
BseMI GCAATG 3 cut(s) 95, 262, 1521
BseRI GAGGAG 5 cut(s) 1047, 1779, 2339, 2646, 3031
BseSI GKGCMC 5 cut(s) 113, 290, 1314, 2440, 2671
BseX3I CGGCCG 1 cut(s) 2777
BseYI CCCAGC 2 cut(s) 1125, 2219
BsgI GTGCAG 3 cut(s) 419, 449, 1434
Bsh1285I CGRYCG 1 cut(s) 2780
BshNI GGYRCC 3 cut(s) 994, 2318, 2668
BshVI ATCGAT 2 cut(s) 2956, 3313
BsiEI CGRYCG 1 cut(s) 2780
BsiHKAI GWGCWC 8 cut(s) 113, 155, 234, 290, 1263, 2440, 2695, 3804
BsiHKCI CYCGRG 1 cut(s) 2873
BsiSI CCGG 8 cut(s) 62, 1889, 2078, 2486, 2780, 2874, 2906, 3928
BslFI GGGAC 6 cut(s) 796, 1108, 1168, 1578, 1719, 2538
BsmAI GTCTC 7 cut(s) 540, 1161, 1650, 1671, 2928, 3055, 3138
BsmBI CGTCTC 2 cut(s) 540, 1161
BsmFI GGGAC 6 cut(s) 796, 1108, 1168, 1578, 1719, 2538
BsmI GAATGC 3 cut(s) 1174, 3784, 3850
Bso31I GGTCTC 2 cut(s) 2928, 3055
BsoBI CYCGRG 1 cut(s) 2873
Bsp119I TTCGAA 2 cut(s) 587, 2856
Bsp13I TCCGGA 1 cut(s) 2077
Bsp143I GATC 9 cut(s) 688, 2290, 2974, 3100, 3322, 3421, 3481, 3697, 3874
Bsp19I CCATGG 1 cut(s) 2746
BspACI CCGC 8 cut(s) 44, 800, 821, 1881, 2382, 2776, 3326, 3472
BspDI ATCGAT 2 cut(s) 2956, 3313
BspEI TCCGGA 1 cut(s) 2077
BspMAI CTGCAG 1 cut(s) 3109
BspMI ACCTGC 3 cut(s) 819, 1282, 1921
BspPI GGATC 4 cut(s) 683, 2298, 3095, 3317
BspT104I TTCGAA 2 cut(s) 587, 2856
BspT107I GGYRCC 3 cut(s) 994, 2318, 2668
BspTNI GGTCTC 2 cut(s) 2928, 3055
BsrBI CCGCTC 1 cut(s) 823
BsrDI GCAATG 3 cut(s) 95, 262, 1521
BsrFI RCCGGY 2 cut(s) 1888, 2779
BssAI RCCGGY 2 cut(s) 1888, 2779
BssMI GATC 9 cut(s) 688, 2290, 2974, 3100, 3322, 3421, 3481, 3697, 3874
BssNI GRCGYC 1 cut(s) 533
BssSI CACGAG 2 cut(s) 2943, 3261
BssT1I CCWWGG 4 cut(s) 2579, 2746, 2990, 3122
Bst2BI CACGAG 2 cut(s) 2943, 3261
Bst6I CTCTTC 2 cut(s) 2133, 3024
BstACI GRCGYC 1 cut(s) 533
BstAPI GCANNNNNTGC 2 cut(s) 760, 2609
BstBI TTCGAA 2 cut(s) 587, 2856
BstDSI CCRYGG 1 cut(s) 2746
BstEII GGTNACC 2 cut(s) 3179, 3455
BstENI CCTNNNNNAGG 3 cut(s) 2463, 2661, 3055
BstH2I RGCGCY 2 cut(s) 1510, 3205
BstHHI GCGC 4 cut(s) 940, 1509, 2653, 3204
BstKTI GATC 9 cut(s) 691, 2293, 2977, 3103, 3325, 3424, 3484, 3700, 3877
BstMAI GTCTC 7 cut(s) 540, 1161, 1650, 1671, 2928, 3055, 3138
BstMBI GATC 9 cut(s) 688, 2290, 2974, 3100, 3322, 3421, 3481, 3697, 3874
BstMCI CGRYCG 1 cut(s) 2780
BstNSI RCATGY 4 cut(s) 2537, 2830, 3533, 3560
BstPAI GACNNNNGTC 1 cut(s) 959
BstPI GGTNACC 2 cut(s) 3179, 3455
BstSFI CTRYAG 5 cut(s) 354, 1173, 1953, 2361, 3105
BstSLI GKGCMC 5 cut(s) 113, 290, 1314, 2440, 2671
BstV2I GAAGAC 1 cut(s) 65
BstX2I RGATCY 1 cut(s) 3100
BstXI CCANNNNNNTGG 1 cut(s) 3317
BstYI RGATCY 1 cut(s) 3100
BstZI CGGCCG 1 cut(s) 2777
Bsu15I ATCGAT 2 cut(s) 2956, 3313
Bsu36I CCTNAGG 1 cut(s) 2520
BsuI GTATCC 2 cut(s) 687, 1797
BsuTUI ATCGAT 2 cut(s) 2956, 3313
BtgI CCRYGG 1 cut(s) 2746
BtrI CACGTC 2 cut(s) 3611, 3781
BtsI GCAGTG 1 cut(s) 2230
BtsIMutI CAGTG 5 cut(s) 113, 534, 644, 1263, 2230
BveI ACCTGC 3 cut(s) 819, 1282, 1921
CaiI CAGNNNCTG 3 cut(s) 476, 3104, 3566
CfoI GCGC 4 cut(s) 940, 1509, 2653, 3204
Cfr10I RCCGGY 2 cut(s) 1888, 2779
Cfr9I CCCGGG 1 cut(s) 2873
ClaI ATCGAT 2 cut(s) 2956, 3313
Csp6I GTAC 8 cut(s) 52, 664, 715, 1284, 1733, 2154, 2372, 3827
CspCI CAANNNNNGTGG 4 cut(s) 352, 387, 1683, 1718
CviQI GTAC 8 cut(s) 52, 664, 715, 1284, 1733, 2154, 2372, 3827
DpnI GATC 9 cut(s) 690, 2292, 2976, 3102, 3324, 3423, 3483, 3699, 3876
DpnII GATC 9 cut(s) 688, 2290, 2974, 3100, 3322, 3421, 3481, 3697, 3874
EaeI YGGCCR 3 cut(s) 2730, 2749, 2777
EagI CGGCCG 1 cut(s) 2777
Eam1104I CTCTTC 2 cut(s) 2133, 3024
EarI CTCTTC 2 cut(s) 2133, 3024
EclXI CGGCCG 1 cut(s) 2777
Eco130I CCWWGG 4 cut(s) 2579, 2746, 2990, 3122
Eco147I AGGCCT 2 cut(s) 2518, 2995
Eco31I GGTCTC 2 cut(s) 2928, 3055
Eco47I GGWCC 4 cut(s) 2525, 2678, 3398, 3491
Eco52I CGGCCG 1 cut(s) 2777
Eco57I CTGAAG 7 cut(s) 453, 965, 2055, 2220, 2337, 2448, 3632
Eco81I CCTNAGG 1 cut(s) 2520
Eco88I CYCGRG 1 cut(s) 2873
Eco91I GGTNACC 2 cut(s) 3179, 3455
EcoNI CCTNNNNNAGG 3 cut(s) 2463, 2661, 3055
EcoO109I RGGNCCY 6 cut(s) 299, 2468, 2525, 3398, 3491, 3563
EcoO65I GGTNACC 2 cut(s) 3179, 3455
EcoRI GAATTC 3 cut(s) 589, 1491, 3338
EcoT14I CCWWGG 4 cut(s) 2579, 2746, 2990, 3122
ErhI CCWWGG 4 cut(s) 2579, 2746, 2990, 3122
Esp3I CGTCTC 2 cut(s) 540, 1161
FalI AAGNNNNNCTT 2 cut(s) 2259, 2291
FaqI GGGAC 6 cut(s) 796, 1108, 1168, 1578, 1719, 2538
FauI CCCGC 1 cut(s) 37
FauNDI CATATG 1 cut(s) 1747
FbaI TGATCA 3 cut(s) 3421, 3697, 3874
FblI GTMKAC 1 cut(s) 81
FspBI CTAG 7 cut(s) 2085, 2313, 2580, 2700, 2991, 3045, 3686
FspI TGCGCA 1 cut(s) 939
GlaI GCGC 4 cut(s) 939, 1508, 2652, 3203
GsaI CCCAGC 2 cut(s) 1129, 2223
GsuI CTGGAG 4 cut(s) 597, 1446, 1487, 2405
HaeII RGCGCY 2 cut(s) 1510, 3205
HapII CCGG 8 cut(s) 62, 1889, 2078, 2486, 2780, 2874, 2906, 3928
HhaI GCGC 4 cut(s) 940, 1509, 2653, 3204
Hin1I GRCGYC 1 cut(s) 533
Hin6I GCGC 4 cut(s) 938, 1507, 2651, 3202
HinP1I GCGC 4 cut(s) 938, 1507, 2651, 3202
HincII GTYRAC 1 cut(s) 3754
HindII GTYRAC 1 cut(s) 3754
HpaII CCGG 8 cut(s) 62, 1889, 2078, 2486, 2780, 2874, 2906, 3928
Hpy166II GTNNAC 9 cut(s) 82, 111, 288, 1468, 2189, 2438, 2681, 3197, 3754
Hpy188III TCNNGA 7 cut(s) 461, 1488, 1866, 2078, 2493, 3005, 3045
Hpy8I GTNNAC 9 cut(s) 82, 111, 288, 1468, 2189, 2438, 2681, 3197, 3754
Hpy99I CGWCG 1 cut(s) 3228
HpyCH4IV ACGT 7 cut(s) 144, 533, 1282, 1440, 1591, 3610, 3780
HpySE526I ACGT 7 cut(s) 144, 533, 1282, 1440, 1591, 3610, 3780
Hsp92I GRCGYC 1 cut(s) 533
HspAI GCGC 4 cut(s) 938, 1507, 2651, 3202
Kpn2I TCCGGA 1 cut(s) 2077
KroI GCCGGC 2 cut(s) 1888, 2779
KroNI GCCGGC 2 cut(s) 1890, 2781
Ksp22I TGATCA 3 cut(s) 3421, 3697, 3874
Kzo9I GATC 9 cut(s) 688, 2290, 2974, 3100, 3322, 3421, 3481, 3697, 3874
LweI GCATC 8 cut(s) 190, 397, 1707, 2299, 2379, 2612, 3261, 3883
MaeI CTAG 7 cut(s) 2085, 2313, 2580, 2700, 2991, 3045, 3686
MaeII ACGT 7 cut(s) 144, 533, 1282, 1440, 1591, 3610, 3780
MaeIII GTNAC 8 cut(s) 187, 523, 529, 1908, 2448, 3035, 3179, 3455
MalI GATC 9 cut(s) 690, 2292, 2976, 3102, 3324, 3423, 3483, 3699, 3876
MbiI CCGCTC 1 cut(s) 823
MboI GATC 9 cut(s) 688, 2290, 2974, 3100, 3322, 3421, 3481, 3697, 3874
MboII GAAGA 9 cut(s) 65, 2022, 2048, 2150, 2276, 2343, 2454, 2981, 3011
MflI RGATCY 1 cut(s) 3100
MlsI TGGCCA 2 cut(s) 2732, 2751
MluNI TGGCCA 2 cut(s) 2732, 2751
MlyI GAGTC 5 cut(s) 1336, 2090, 2120, 3081, 3698
MmeI TCCRAC 3 cut(s) 650, 1552, 1996
Mox20I TGGCCA 2 cut(s) 2732, 2751
MroI TCCGGA 1 cut(s) 2077
MroNI GCCGGC 2 cut(s) 1888, 2779
MroXI GAANNNNTTC 1 cut(s) 2924
MscI TGGCCA 2 cut(s) 2732, 2751
MseI TTAA 6 cut(s) 1974, 2504, 2895, 2952, 3155, 3863
MslI CAYNNNNRTG 5 cut(s) 279, 1629, 2751, 3165, 3384
Msp20I TGGCCA 2 cut(s) 2732, 2751
MspA1I CMGCKG 3 cut(s) 485, 2776, 3110
MspI CCGG 8 cut(s) 62, 1889, 2078, 2486, 2780, 2874, 2906, 3928
Mva1269I GAATGC 3 cut(s) 1174, 3784, 3850
NaeI GCCGGC 2 cut(s) 1890, 2781
NciI CCSGG 5 cut(s) 62, 2487, 2874, 2875, 3929
NcoI CCATGG 1 cut(s) 2746
NdeI CATATG 1 cut(s) 1747
NdeII GATC 9 cut(s) 688, 2290, 2974, 3100, 3322, 3421, 3481, 3697, 3874
NgoMIV GCCGGC 2 cut(s) 1888, 2779
NmuCI GTSAC 4 cut(s) 187, 529, 3179, 3455
NsbI TGCGCA 1 cut(s) 939
NspI RCATGY 4 cut(s) 2537, 2830, 3533, 3560
NspV TTCGAA 2 cut(s) 587, 2856
OliI CACNNNNGTG 1 cut(s) 279
PaqCI CACCTGC 1 cut(s) 1282
PasI CCCWGGG 1 cut(s) 1597
PceI AGGCCT 2 cut(s) 2518, 2995
PciI ACATGT 1 cut(s) 2533
PctI GAATGC 3 cut(s) 1174, 3784, 3850
PdiI GCCGGC 2 cut(s) 1890, 2781
PdmI GAANNNNTTC 1 cut(s) 2924
PflFI GACNNNGTC 1 cut(s) 3228
PflMI CCANNNNNTGG 9 cut(s) 176, 779, 1727, 2687, 3191, 3263, 3614, 3665, 3811
PfoI TCCNGGA 1 cut(s) 679
PleI GAGTC 5 cut(s) 1336, 2089, 2119, 3080, 3697
PpsI GAGTC 5 cut(s) 1336, 2089, 2119, 3080, 3697
PpuMI RGGWCCY 3 cut(s) 2525, 3398, 3491
PscI ACATGT 1 cut(s) 2533
PshAI GACNNNNGTC 1 cut(s) 959
PshBI ATTAAT 2 cut(s) 2952, 3155
PsiI TTATAA 1 cut(s) 2637
Psp1406I AACGTT 1 cut(s) 1440
Psp5II RGGWCCY 3 cut(s) 2525, 3398, 3491
PspEI GGTNACC 2 cut(s) 3179, 3455
PspFI CCCAGC 2 cut(s) 1125, 2219
PspPPI RGGWCCY 3 cut(s) 2525, 3398, 3491
PsrI GAACNNNNNNTAC 2 cut(s) 1752, 1784
PstI CTGCAG 1 cut(s) 3109
PstNI CAGNNNCTG 3 cut(s) 476, 3104, 3566
PsuI RGATCY 1 cut(s) 3100
PsyI GACNNNGTC 1 cut(s) 3228
PvuII CAGCTG 2 cut(s) 485, 3110
RsaI GTAC 8 cut(s) 53, 665, 716, 1285, 1734, 2155, 2373, 3828
RsaNI GTAC 8 cut(s) 52, 664, 715, 1284, 1733, 2154, 2372, 3827
RseI CAYNNNNRTG 5 cut(s) 279, 1629, 2751, 3165, 3384
SaqAI TTAA 6 cut(s) 1974, 2504, 2895, 2952, 3155, 3863
Sau3AI GATC 9 cut(s) 688, 2290, 2974, 3100, 3322, 3421, 3481, 3697, 3874
ScaI AGTACT 5 cut(s) 53, 665, 716, 1734, 2155
SchI GAGTC 5 cut(s) 1336, 2090, 2120, 3081, 3698
SfaNI GCATC 8 cut(s) 190, 397, 1707, 2299, 2379, 2612, 3261, 3883
SfcI CTRYAG 5 cut(s) 354, 1173, 1953, 2361, 3105
SfuI TTCGAA 2 cut(s) 587, 2856
SinI GGWCC 4 cut(s) 2525, 2678, 3398, 3491
SmaI CCCGGG 1 cut(s) 2875
SmiMI CAYNNNNRTG 5 cut(s) 279, 1629, 2751, 3165, 3384
SmlI CTYRAG 6 cut(s) 600, 882, 1059, 1302, 2978, 3703
SmoI CTYRAG 6 cut(s) 600, 882, 1059, 1302, 2978, 3703
SseBI AGGCCT 2 cut(s) 2518, 2995
SsiI CCGC 8 cut(s) 44, 800, 821, 1881, 2382, 2776, 3326, 3472
SspI AATATT 1 cut(s) 1550
SspMI CTAG 7 cut(s) 2085, 2313, 2580, 2700, 2991, 3045, 3686
StuI AGGCCT 2 cut(s) 2518, 2995
StyI CCWWGG 4 cut(s) 2579, 2746, 2990, 3122
TaiI ACGT 7 cut(s) 147, 536, 1285, 1443, 1594, 3613, 3783
TaqI TCGA 9 cut(s) 587, 1214, 1637, 2856, 2956, 3054, 3223, 3313, 3553
TatI WGTACW 6 cut(s) 51, 663, 714, 1732, 2153, 2371
TauI GCSGC 2 cut(s) 2385, 2779
Tru1I TTAA 6 cut(s) 1974, 2504, 2895, 2952, 3155, 3863
Tru9I TTAA 6 cut(s) 1974, 2504, 2895, 2952, 3155, 3863
TscAI CASTG 5 cut(s) 113, 534, 644, 1263, 2230
TseFI GTSAC 4 cut(s) 187, 529, 3179, 3455
Tsp45I GTSAC 4 cut(s) 187, 529, 3179, 3455
TspMI CCCGGG 1 cut(s) 2873
TspRI CASTG 5 cut(s) 113, 534, 644, 1263, 2230
Tth111I GACNNNGTC 1 cut(s) 3228
Van91I CCANNNNNTGG 9 cut(s) 176, 779, 1727, 2687, 3191, 3263, 3614, 3665, 3811
VneI GTGCAC 3 cut(s) 109, 286, 2436
VpaK11BI GGWCC 4 cut(s) 2525, 2678, 3398, 3491
VspI ATTAAT 2 cut(s) 2952, 3155
XagI CCTNNNNNAGG 3 cut(s) 2463, 2661, 3055
XapI RAATTY 8 cut(s) 346, 589, 1008, 1491, 1861, 1933, 3094, 3338
XbaI TCTAGA 1 cut(s) 3044
XceI RCATGY 4 cut(s) 2537, 2830, 3533, 3560
XcmI CCANNNNNNNNNTGG 1 cut(s) 677
XmaI CCCGGG 1 cut(s) 2873
XmaJI CCTAGG 2 cut(s) 2579, 2990
XmiI GTMKAC 1 cut(s) 81
XmnI GAANNNNTTC 1 cut(s) 2924
XspI CTAG 7 cut(s) 2085, 2313, 2580, 2700, 2991, 3045, 3686
ZraI GACGTC 1 cut(s) 534
ZrmI AGTACT 5 cut(s) 53, 665, 716, 1734, 2155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.