Rroxscaffold_1G00013020

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002401
Physical Location & Seq
Forward (+)
16051032 .. 16060680
9649 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00013020.1

Sequence Viewer

Length: 3960 bp
ATGGAAGCAATCTTTGAATCAGAAAAACCGCTAAACAAGCTGGTGGATTATATTGATGCACTAAGCATGGGATTTGATAATGAGTGCATACTGAACATCCAATCTCTTGCGGGAGAGTACTTTTGCCCGGTCTGTCGTCTTCTTGTCTACCCAAATGAAGCATTGCAGTCACAGTGCACTCATCTATACTGTAAGCCCTGTTTGACATACGTCGTGAGCACTACCAAAGCGTGTCCATACGATGGCTACTTAGTGACAGAAGCCGATGCGAAGCCCCTCGTTGAATCAAATAAAGCGCTCGCTGAAACCATAGGGAAAATAGCAGTTCATTGCCTGTATCACAGGAGTGGGTGCACATGGCAGGGGCCTTTATCTGATTGCACATCTCATTGTTCTGGTTGTGCCTTTGGAAATTCTCCTGTAGTCTGCAACAGGTGTGGAATCCAAATTGTGCATCGTCAAGTGCAGGAACACGCACAAAGTTGCCCTGGTGTGCAGCCCCAAGCACAGCAGGCAGAGGGTGTTCCAGACACTTCAGCCTCTGGCACAGCTGCTACTGCCAATCAGAACCAGGCTGCCACTCAGACAGGTACAGTGACATCTCAGACACAGGCTCCCCAAACTGCAAGTGGCACTATACCTGGAGCTGATTCGAATTCTCAGGCTCAAGCTGTTGTTCAAGCTGCTGCTCCGACTGCTGACCAGTGGTATCAGCAACAATATCAGCAGTACTACCAGCAGTATCCTGGATATGATCCATACCAACAACAGTATCAGCAGTACTATCCCTATCAGCAGCCAGCAATTCCACAACCACAGCAGCCTTTGCAGGCTAATCCACCCTATGGGACTGTTCAGCCTCAACCGCAGGCATACCTGCAACCTCCGCTCCTACCTCAACCTCAACCTCAACCTCAACCTCAACCACAGACCCAACCATCTCAATCTCAAGCTCAACCCCAACCGCAAGTTCAGCCCCCAATTATGCAGGCTCATATGGCTGCGCATACTCAAAACCAGACATCAGTTAATCAACAGCTTCAACTTCAGCCTGCTATGGCACCACATTCACAAATTCAAGCACAGTCTTACCCATCAGCTCATGGGCAGGCTCAACTTCCACCTCAAGCATATTTCCAAGTCCAAATGCCTCAATATCAGCAGCCACAAGTTCAGCAGCTTACACAGTCCCAGCTTCAACAGAATCCCCAACTTCACCCGTCTCAGCCCATGAATGCTACAGTCCAGTCCCAGACACAGCTCCCATCATCTAATTCGTCGACTGGCAATCATTTGTTCCCTCAATCTCACCTTCCTCAACCAGTGCTCTCAGCAGCCTCCCAGCAACGTACTATGCAGGTGCAATCTCAAGGAGTGCCCAACTCTCAGTCGCAAAACCATGTGCAGACTCAGATTCAGTTCCCTCAGCAACCCCTTTGCGACCACCCCCTTCCAAACCAGCAGCAGACAGCCTTGTTGCCTTCACCTAACGTCATCTCTGCACAACAGCCTCCAGTTCACTCCTTTGCTCAACAATCTGGAATTCCTGTTCTCCAGCGCCCTGCAATGCAGCCAGTCCAACAGCTAAATCCTCAGCAATATTTCCACATACAACCCCCCACATTGTCGCAATTACGTCCCCAGGGCCAATCTCAATCATTCCCACAACATATTCGTGCCTCGAACCAGTCTCAGCAGAATGTTGTATTGACTCAGGGCATGCAGCATATTCAGCCCTCAAATCTTGTGGGACGGCCTATGATGCCAAGTCATGGAGTACTACCTCAGCCATATGCACAAACAGTAGGAGGAGTTCTGCCAAGACCAATGTATCCTGCTCTAAACCATCAATCTACAAATCAGAATAATATAGGCAGAACCAACAACCAAGTGCAACTGGGAGCAAATTCAAGACCAACAATGACCGCAAGGCCAGCAGAGAAAGAAGCTGAGTTACCTGCTAATAATGTTGCACAGAATTTGGGTGTATTGTTTGCTGTAGGTGCTGATTCCGAGGTTAAAACTGTGAAATCTGAGGTGGATATGAAGGCCATTGATGTTGGAAATAAACCTTCTTCTGAAGATAGAAGTTATCAGGGTAATACATCTGCCAAAGAGATTCCGGAGTCTAGGGGGATGTTGGGGGTGAATGGAGAGTCTGAAAGTAAGCCAACATTGAAGGAAGAGGGTGTAAATAGTACTTTGGAGGATTCAAGTAATGATAAATTGGGTGAACTTGTAGCTGAAGGTGCAAAAGATGTTCCCAGCAGTGGCATGAAACAGGGGGAGTGTAAAGAAATGCCGTCAGAAGAAGCACAACTTCATGGAGTAAAGGATCAAAAATTGCAAAAAGATGCTAGTGGCACCGAGGAGGGTTCCCAAACTGTTTCTACTTCTTCAGCTCCTATAGGTCAAGTACAAGCTGGCGGCTTAATGCAACCTTCATATCCTGGTTCTGCTATACTCCAGCAAAGGCCAGGTGCATCTCCTTCGTTACAAGTGCCTTCTTCAGGGCCTCCACATCATATACCGGGTTCTGGACAACCCTTAATTCATGCAAGGCCTCAGGAACCTGGACATGTGCCTGGGCAGGCTTCTCATCTATCTGAGCATTTTCAATCACCTAGGGGAAACCTGGGATTTGCAGCATCATCTGCAGGTACAAGTCAGCATGGGCCTTATAACCAAAGCCATGGGCCTCCTCATCCAGGGGCACCCAGAGGTCCACCATTTGGAGCACCACCTAGTGCTTTTGATTTTCATGGGGGTATAATGGCCCGAGCAACACCCCATGGCCATGAAGGTCAGAGGGGTCAGCAGCGGCCAGCTTTTCAGATGGAACAGGGAGCAACTGGACAGCCATCCTCAAACATGCTCAGAATGAATGGAAATCCAGGCTTCGAATCCTTATCAACTCCCGGGTTGAGGGATGAAAGATTTAAGGCTTTGCCGGATGGGAGGTTGAATCCCTTCCCAGGAGACCCAACTCGTGTGATTAATCGATTAGGGTTTGAAGATGATCTCAAGCAATTCCCTAGGCCTTCTTATCTGGATTCTGAGCCTCTTCCAAAATTAGGGAGTTACTCCTCTAGAGCCTTCGATAGGAGACCTCTTGGAGTCAATTATGATACTCGTCTAAATTCAGATCCTGCAGCTGGCTCTGCTCCAAGGTTCCTGTCTCCCTATGGTCATGGTGGATTGATTCATGCCAATGATACAGGTGGTCACCCAGATTTTGGTGGACAGCGCCTTATGGATGGTTTGGCTCGACGAAGTCCAATCAGAGATTACCCTGGCATCCCATCTCGTGGATTTAGGGGATTTGGCCCTGATGATTTTGATGGCAGGGATTTCCATCGATTTGGTGATCCGCTTGGCAGGGAATTCCATGAGAATAGGTTTCCTAATATGCACAGTCATTTTCATAGGGGTGAGTTTGAGGGTCCTGGTAATATGCGAATGGATGATCGTATGAGGAACGATTTGATTGGTCACCCTGGTCATCTGCGGAGGGGAGATCATTTGGGTCCTCATAACTTGCCTGGTCATTTGCATTTGCGAGAGCATGTGGGTTTTGGTGTCCATCCTCGACATGCAGGGCCTGGGAGCTTTGAATCATTTATTGGCAACAGGGCAAGTCATCCACGTCTTGGCGAGCCTGGATTTAGGAGCAGCTTTTCACTTCAGAGATTTCCAAATGATGGAACTTATACAGGAGAGCTAGAGTCTTTTGATCACTCAAGGAAAAGGAAACCAGCCAGCATGGGATGGTGTCGTATCTGTAAAGTTGACTGTGAAACAGTTGAGGGCTTGGACGTGCATTCACAAACAAGGGAGCACCAGAGAATGGCTATGGAAATGGTACAAAGTATCAAGCAGAATGCCAAAAAACAGAAATTATCTTTTCAATTCGACCCGTCTTTTTTCGGCGAAGCAGACTTCATAAGGGCTGAAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000793 GO:0000794 GO:0001701 GO:0001756 GO:0001932 GO:0002020 GO:0002791 GO:0002793 GO:0003002 GO:0003007 GO:0003008 GO:0003012 GO:0003013 GO:0003015 GO:0003300 GO:0003674 GO:0003779 GO:0003824 GO:0004672 GO:0004674 GO:0004713 GO:0005198 GO:0005200 GO:0005488 GO:0005509 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005856 GO:0005859 GO:0005865 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006936 GO:0006941 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0007399 GO:0007417 GO:0007420 GO:0007507 GO:0007512 GO:0007517 GO:0008015 GO:0008092 GO:0008150 GO:0008152 GO:0008307 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009653 GO:0009790 GO:0009792 GO:0009887 GO:0009888 GO:0009893 GO:0009952 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010604 GO:0010628 GO:0010737 GO:0010927 GO:0014706 GO:0014866 GO:0014896 GO:0014897 GO:0015629 GO:0016043 GO:0016301 GO:0016310 GO:0016459 GO:0016460 GO:0016740 GO:0016772 GO:0016773 GO:0018108 GO:0018193 GO:0018212 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0021591 GO:0022607 GO:0023052 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030036 GO:0030154 GO:0030239 GO:0030240 GO:0030241 GO:0030506 GO:0031032 GO:0031033 GO:0031034 GO:0031323 GO:0031399 GO:0031430 GO:0031433 GO:0031672 GO:0031674 GO:0031974 GO:0031981 GO:0032268 GO:0032501 GO:0032502 GO:0032879 GO:0032880 GO:0032989 GO:0032991 GO:0033058 GO:0034622 GO:0035051 GO:0035265 GO:0035282 GO:0035556 GO:0035994 GO:0035995 GO:0036211 GO:0036379 GO:0040007 GO:0040011 GO:0042221 GO:0042325 GO:0042692 GO:0042802 GO:0042805 GO:0043009 GO:0043056 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043292 GO:0043412 GO:0043549 GO:0043621 GO:0043933 GO:0044057 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044449 GO:0044464 GO:0044877 GO:0045214 GO:0045859 GO:0046872 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048589 GO:0048644 GO:0048646 GO:0048729 GO:0048731 GO:0048738 GO:0048739 GO:0048747 GO:0048769 GO:0048856 GO:0048869 GO:0050708 GO:0050714 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0050982 GO:0051015 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051146 GO:0051171 GO:0051174 GO:0051222 GO:0051223 GO:0051239 GO:0051246 GO:0051338 GO:0051371 GO:0051393 GO:0051592 GO:0051606 GO:0051716 GO:0055001 GO:0055002 GO:0055003 GO:0055006 GO:0055007 GO:0055008 GO:0055013 GO:0060047 GO:0060048 GO:0060255 GO:0060322 GO:0060415 GO:0060419 GO:0060429 GO:0060537 GO:0061053 GO:0061061 GO:0065003 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070925 GO:0071688 GO:0071704 GO:0071840 GO:0072359 GO:0080090 GO:0090087 GO:0090257 GO:0097435 GO:0097493 GO:0099080 GO:0099081 GO:0099512 GO:0140096 GO:1901077 GO:1901564 GO:1901897 GO:1903530 GO:1903532 GO:1904951
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1319

Amino Acids

144.03

Weight (kDa)

6.64

Isoelectric Point (pI)

59.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2682
AarI CACCTGC 1 cut(s) 1348
Acc16I TGCGCA 1 cut(s) 1005
Acc36I ACCTGC 4 cut(s) 885, 1348, 1966, 2648
AccB1I GGYRCC 3 cut(s) 1060, 2363, 2713
AccB7I CCANNNNNTGG 9 cut(s) 242, 845, 1772, 2732, 3236, 3308, 3650, 3701, 3847
AccBSI CCGCTC 1 cut(s) 889
AccI GTMKAC 2 cut(s) 147, 1280
AccIII TCCGGA 1 cut(s) 2122
AclWI GGATC 4 cut(s) 749, 2343, 3140, 3362
AcoI YGGCCR 2 cut(s) 2794, 2822
AcsI RAATTY 8 cut(s) 412, 655, 1074, 1542, 1906, 1978, 3139, 3383
AcuI CTGAAG 7 cut(s) 519, 1031, 2100, 2265, 2382, 2493, 3668
AdeI CACNNNGTG 1 cut(s) 2747
AfeI AGCGCT 1 cut(s) 297
AflIII ACRYGT 1 cut(s) 2578
AjiI CACGTC 2 cut(s) 3647, 3817
AjuI GAANNNNNNNTTGG 4 cut(s) 954, 986, 3606, 3638
AleI CACNNNNGTG 1 cut(s) 345
AloI GAACNNNNNNTCC 2 cut(s) 1533, 1565
Alw21I GWGCWC 6 cut(s) 179, 221, 356, 1329, 2740, 3840
Alw26I GTCTC 5 cut(s) 1227, 1695, 2973, 3100, 3183
Alw44I GTGCAC 2 cut(s) 175, 352
AlwI GGATC 4 cut(s) 749, 2343, 3140, 3362
AlwNI CAGNNNCTG 3 cut(s) 542, 3149, 3602
Ama87I CYCGRG 2 cut(s) 2778, 2918
Aor13HI TCCGGA 1 cut(s) 2122
Aor51HI AGCGCT 1 cut(s) 297
ApaLI GTGCAC 2 cut(s) 175, 352
ApoI RAATTY 8 cut(s) 412, 655, 1074, 1542, 1906, 1978, 3139, 3383
AseI ATTAAT 1 cut(s) 2997
Asp700I GAANNNNTTC 1 cut(s) 2969
AspA2I CCTAGG 2 cut(s) 2624, 3035
AspLEI GCGC 4 cut(s) 298, 1006, 1560, 3249
AsuC2I CCSGG 4 cut(s) 128, 2532, 2919, 2920
AsuII TTCGAA 2 cut(s) 653, 2901
AvaI CYCGRG 2 cut(s) 2778, 2918
AvaII GGWCC 3 cut(s) 2723, 3443, 3527
AvrII CCTAGG 2 cut(s) 2624, 3035
AxyI CCTNAGG 1 cut(s) 2565
BaeGI GKGCMC 4 cut(s) 179, 356, 1380, 2716
BaeI ACNNNNGTAYC 4 cut(s) 755, 788, 1814, 1847
BalI TGGCCA 1 cut(s) 2796
BanI GGYRCC 3 cut(s) 1060, 2363, 2713
BarI GAAGNNNNNNTAC 2 cut(s) 1938, 1970
BauI CACGAG 2 cut(s) 2988, 3306
BbsI GAAGAC 1 cut(s) 131
Bbv12I GWGCWC 6 cut(s) 179, 221, 356, 1329, 2740, 3840
BbvCI CCTCAGC 3 cut(s) 1425, 1593, 1785
BceAI ACGGC 2 cut(s) 1769, 2287
BcgI CGANNNNNNTGC 2 cut(s) 2472, 2506
BciVI GTATCC 2 cut(s) 753, 1842
BclI TGATCA 1 cut(s) 3733
BcnI CCSGG 4 cut(s) 128, 2532, 2919, 2920
BcoDI GTCTC 5 cut(s) 1227, 1695, 2973, 3100, 3183
BfaI CTAG 7 cut(s) 2130, 2358, 2625, 2745, 3036, 3090, 3722
BfmI CTRYAG 6 cut(s) 420, 1239, 1998, 2406, 2655, 3150
BfoI RGCGCY 3 cut(s) 299, 1561, 3250
BfuAI ACCTGC 4 cut(s) 885, 1348, 1966, 2648
BfuI GTATCC 2 cut(s) 753, 1842
BlnI CCTAGG 2 cut(s) 2624, 3035
BmcAI AGTACT 5 cut(s) 119, 731, 782, 1779, 2200
Bme18I GGWCC 3 cut(s) 2723, 3443, 3527
BmeT110I CYCGRG 2 cut(s) 2778, 2918
BmgBI CACGTC 2 cut(s) 3647, 3817
BmrI ACTGGG 1 cut(s) 1907
BmsI GCATC 8 cut(s) 46, 256, 463, 1752, 2344, 2492, 2657, 3306
BmuI ACTGGG 1 cut(s) 1907
BoxI GACNNNNGTC 1 cut(s) 209
BpiI GAAGAC 1 cut(s) 131
BpmI CTGGAG 4 cut(s) 663, 1497, 1538, 2450
Bpu10I CCTNAGC 3 cut(s) 1425, 1593, 1785
Bpu14I TTCGAA 2 cut(s) 653, 2901
BpuEI CTTGAG 6 cut(s) 651, 933, 1110, 1353, 3008, 3724
BpuMI CCSGG 4 cut(s) 128, 2532, 2919, 2920
Bsa29I ATCGAT 2 cut(s) 3001, 3358
BsaBI GATNNNNATC 1 cut(s) 3354
BsaI GGTCTC 2 cut(s) 2973, 3100
BsaWI WCCGGW 1 cut(s) 2122
BsaXI ACNNNNNCTCC 4 cut(s) 598, 628, 873, 903
Bse1I ACTGG 9 cut(s) 703, 1246, 1288, 1322, 1514, 1574, 1687, 1902, 2857
Bse21I CCTNAGG 1 cut(s) 2565
Bse3DI GCAATG 3 cut(s) 161, 328, 1572
Bse8I GATNNNNATC 1 cut(s) 3354
BseAI TCCGGA 1 cut(s) 2122
BseCI ATCGAT 2 cut(s) 3001, 3358
BseJI GATNNNNATC 1 cut(s) 3354
BseMI GCAATG 3 cut(s) 161, 328, 1572
BseNI ACTGG 9 cut(s) 703, 1246, 1288, 1322, 1514, 1574, 1687, 1902, 2857
BseRI GAGGAG 4 cut(s) 1824, 2384, 2691, 3076
BseSI GKGCMC 4 cut(s) 179, 356, 1380, 2716
BseYI CCCAGC 3 cut(s) 1191, 1341, 2264
BsgI GTGCAG 4 cut(s) 485, 515, 1424, 1485
BshNI GGYRCC 3 cut(s) 1060, 2363, 2713
BshVI ATCGAT 2 cut(s) 3001, 3358
BsiHKAI GWGCWC 6 cut(s) 179, 221, 356, 1329, 2740, 3840
BsiHKCI CYCGRG 2 cut(s) 2778, 2918
BsiSI CCGG 5 cut(s) 128, 2123, 2531, 2919, 2951
BslFI GGGAC 5 cut(s) 862, 1174, 1234, 1623, 1764
BsmAI GTCTC 5 cut(s) 1227, 1695, 2973, 3100, 3183
BsmBI CGTCTC 1 cut(s) 1227
BsmFI GGGAC 5 cut(s) 862, 1174, 1234, 1623, 1764
BsmI GAATGC 3 cut(s) 1240, 3820, 3886
Bso31I GGTCTC 2 cut(s) 2973, 3100
BsoBI CYCGRG 2 cut(s) 2778, 2918
Bsp119I TTCGAA 2 cut(s) 653, 2901
Bsp1286I GDGCHC 8 cut(s) 179, 221, 356, 1329, 1380, 2716, 2740, 3840
Bsp13I TCCGGA 1 cut(s) 2122
Bsp143I GATC 8 cut(s) 754, 2335, 3019, 3145, 3367, 3466, 3517, 3733
Bsp19I CCATGG 2 cut(s) 2692, 2791
BspDI ATCGAT 2 cut(s) 3001, 3358
BspEI TCCGGA 1 cut(s) 2122
BspMAI CTGCAG 2 cut(s) 2659, 3154
BspMI ACCTGC 4 cut(s) 885, 1348, 1966, 2648
BspPI GGATC 4 cut(s) 749, 2343, 3140, 3362
BspT104I TTCGAA 2 cut(s) 653, 2901
BspT107I GGYRCC 3 cut(s) 1060, 2363, 2713
BspTNI GGTCTC 2 cut(s) 2973, 3100
BsrBI CCGCTC 1 cut(s) 889
BsrDI GCAATG 3 cut(s) 161, 328, 1572
BsrI ACTGG 9 cut(s) 703, 1246, 1288, 1322, 1514, 1574, 1687, 1902, 2857
BssMI GATC 8 cut(s) 754, 2335, 3019, 3145, 3367, 3466, 3517, 3733
BssSI CACGAG 2 cut(s) 2988, 3306
BssT1I CCWWGG 5 cut(s) 2624, 2692, 2791, 3035, 3167
Bst2BI CACGAG 2 cut(s) 2988, 3306
Bst6I CTCTTC 2 cut(s) 2178, 3069
BstAPI GCANNNNNTGC 2 cut(s) 826, 2654
BstBI TTCGAA 2 cut(s) 653, 2901
BstDSI CCRYGG 2 cut(s) 2692, 2791
BstEII GGTNACC 2 cut(s) 3224, 3491
BstENI CCTNNNNNAGG 3 cut(s) 2508, 2706, 3100
BstH2I RGCGCY 3 cut(s) 299, 1561, 3250
BstHHI GCGC 4 cut(s) 298, 1006, 1560, 3249
BstKTI GATC 8 cut(s) 757, 2338, 3022, 3148, 3370, 3469, 3520, 3736
BstMAI GTCTC 5 cut(s) 1227, 1695, 2973, 3100, 3183
BstMBI GATC 8 cut(s) 754, 2335, 3019, 3145, 3367, 3466, 3517, 3733
BstNSI RCATGY 5 cut(s) 1723, 2582, 2875, 3569, 3596
BstPAI GACNNNNGTC 1 cut(s) 209
BstPI GGTNACC 2 cut(s) 3224, 3491
BstSFI CTRYAG 6 cut(s) 420, 1239, 1998, 2406, 2655, 3150
BstSLI GKGCMC 4 cut(s) 179, 356, 1380, 2716
BstV2I GAAGAC 1 cut(s) 131
BstX2I RGATCY 1 cut(s) 3145
BstXI CCANNNNNNTGG 2 cut(s) 2693, 3362
BstYI RGATCY 1 cut(s) 3145
Bsu15I ATCGAT 2 cut(s) 3001, 3358
Bsu36I CCTNAGG 1 cut(s) 2565
BsuI GTATCC 2 cut(s) 753, 1842
BsuTUI ATCGAT 2 cut(s) 3001, 3358
BtgI CCRYGG 2 cut(s) 2692, 2791
BtrI CACGTC 2 cut(s) 3647, 3817
BtsI GCAGTG 1 cut(s) 2275
BtsIMutI CAGTG 5 cut(s) 179, 600, 710, 1329, 2275
BveI ACCTGC 4 cut(s) 885, 1348, 1966, 2648
CaiI CAGNNNCTG 3 cut(s) 542, 3149, 3602
CfoI GCGC 4 cut(s) 298, 1006, 1560, 3249
Cfr9I CCCGGG 1 cut(s) 2918
ClaI ATCGAT 2 cut(s) 3001, 3358
CspCI CAANNNNNGTGG 4 cut(s) 418, 453, 1728, 1763
DpnI GATC 8 cut(s) 756, 2337, 3021, 3147, 3369, 3468, 3519, 3735
DpnII GATC 8 cut(s) 754, 2335, 3019, 3145, 3367, 3466, 3517, 3733
DraIII CACNNNGTG 1 cut(s) 2747
EaeI YGGCCR 2 cut(s) 2794, 2822
Eam1104I CTCTTC 2 cut(s) 2178, 3069
EarI CTCTTC 2 cut(s) 2178, 3069
Eco130I CCWWGG 5 cut(s) 2624, 2692, 2791, 3035, 3167
Eco147I AGGCCT 2 cut(s) 2563, 3040
Eco31I GGTCTC 2 cut(s) 2973, 3100
Eco47I GGWCC 3 cut(s) 2723, 3443, 3527
Eco47III AGCGCT 1 cut(s) 297
Eco57I CTGAAG 7 cut(s) 519, 1031, 2100, 2265, 2382, 2493, 3668
Eco81I CCTNAGG 1 cut(s) 2565
Eco88I CYCGRG 2 cut(s) 2778, 2918
Eco91I GGTNACC 2 cut(s) 3224, 3491
EcoNI CCTNNNNNAGG 3 cut(s) 2508, 2706, 3100
EcoO109I RGGNCCY 5 cut(s) 365, 2513, 3443, 3527, 3599
EcoO65I GGTNACC 2 cut(s) 3224, 3491
EcoRI GAATTC 3 cut(s) 655, 1542, 3383
EcoT14I CCWWGG 5 cut(s) 2624, 2692, 2791, 3035, 3167
ErhI CCWWGG 5 cut(s) 2624, 2692, 2791, 3035, 3167
Esp3I CGTCTC 1 cut(s) 1227
FalI AAGNNNNNCTT 2 cut(s) 2304, 2336
FaqI GGGAC 5 cut(s) 862, 1174, 1234, 1623, 1764
FauI CCCGC 1 cut(s) 103
FauNDI CATATG 2 cut(s) 996, 1792
FbaI TGATCA 1 cut(s) 3733
FblI GTMKAC 2 cut(s) 147, 1280
FspBI CTAG 7 cut(s) 2130, 2358, 2625, 2745, 3036, 3090, 3722
FspI TGCGCA 1 cut(s) 1005
GlaI GCGC 4 cut(s) 297, 1005, 1559, 3248
GsaI CCCAGC 3 cut(s) 1195, 1345, 2268
GsuI CTGGAG 4 cut(s) 663, 1497, 1538, 2450
HaeII RGCGCY 3 cut(s) 299, 1561, 3250
HapII CCGG 5 cut(s) 128, 2123, 2531, 2919, 2951
HhaI GCGC 4 cut(s) 298, 1006, 1560, 3249
Hin6I GCGC 4 cut(s) 296, 1004, 1558, 3247
HinP1I GCGC 4 cut(s) 296, 1004, 1558, 3247
HincII GTYRAC 2 cut(s) 1281, 3790
HindII GTYRAC 2 cut(s) 1281, 3790
HpaII CCGG 5 cut(s) 128, 2123, 2531, 2919, 2951
Hpy166II GTNNAC 9 cut(s) 148, 177, 354, 1281, 1519, 2234, 2726, 3242, 3790
Hpy188III TCNNGA 9 cut(s) 214, 527, 1539, 1911, 2123, 2538, 2567, 3050, 3090
Hpy8I GTNNAC 9 cut(s) 148, 177, 354, 1281, 1519, 2234, 2726, 3242, 3790
Hpy99I CGWCG 3 cut(s) 215, 1282, 3273
HpyCH4IV ACGT 6 cut(s) 210, 1348, 1491, 1636, 3646, 3816
HpySE526I ACGT 6 cut(s) 210, 1348, 1491, 1636, 3646, 3816
HspAI GCGC 4 cut(s) 296, 1004, 1558, 3247
Kpn2I TCCGGA 1 cut(s) 2122
Ksp22I TGATCA 1 cut(s) 3733
Kzo9I GATC 8 cut(s) 754, 2335, 3019, 3145, 3367, 3466, 3517, 3733
LweI GCATC 8 cut(s) 46, 256, 463, 1752, 2344, 2492, 2657, 3306
MaeI CTAG 7 cut(s) 2130, 2358, 2625, 2745, 3036, 3090, 3722
MaeII ACGT 6 cut(s) 210, 1348, 1491, 1636, 3646, 3816
MaeIII GTNAC 8 cut(s) 168, 253, 595, 1953, 2493, 3080, 3224, 3491
MalI GATC 8 cut(s) 756, 2337, 3021, 3147, 3369, 3468, 3519, 3735
MbiI CCGCTC 1 cut(s) 889
MboI GATC 8 cut(s) 754, 2335, 3019, 3145, 3367, 3466, 3517, 3733
MboII GAAGA 9 cut(s) 131, 2067, 2093, 2195, 2321, 2388, 2499, 3026, 3056
MflI RGATCY 1 cut(s) 3145
MhlI GDGCHC 8 cut(s) 179, 221, 356, 1329, 1380, 2716, 2740, 3840
MlsI TGGCCA 1 cut(s) 2796
MluNI TGGCCA 1 cut(s) 2796
MlyI GAGTC 6 cut(s) 1402, 1705, 2135, 2165, 3126, 3734
MmeI TCCRAC 3 cut(s) 716, 1603, 2041
Mox20I TGGCCA 1 cut(s) 2796
MroI TCCGGA 1 cut(s) 2122
MroXI GAANNNNTTC 1 cut(s) 2969
MscI TGGCCA 1 cut(s) 2796
MseI TTAA 6 cut(s) 1029, 2019, 2432, 2549, 2940, 2997
MslI CAYNNNNRTG 5 cut(s) 345, 1674, 2796, 3210, 3429
Msp20I TGGCCA 1 cut(s) 2796
MspA1I CMGCKG 3 cut(s) 551, 2821, 3155
MspI CCGG 5 cut(s) 128, 2123, 2531, 2919, 2951
Mva1269I GAATGC 3 cut(s) 1240, 3820, 3886
NciI CCSGG 4 cut(s) 128, 2532, 2919, 2920
NcoI CCATGG 2 cut(s) 2692, 2791
NdeI CATATG 2 cut(s) 996, 1792
NdeII GATC 8 cut(s) 754, 2335, 3019, 3145, 3367, 3466, 3517, 3733
NmuCI GTSAC 5 cut(s) 168, 253, 595, 3224, 3491
NsbI TGCGCA 1 cut(s) 1005
NspI RCATGY 5 cut(s) 1723, 2582, 2875, 3569, 3596
NspV TTCGAA 2 cut(s) 653, 2901
OliI CACNNNNGTG 1 cut(s) 345
PaeI GCATGC 1 cut(s) 1723
PaqCI CACCTGC 1 cut(s) 1348
PasI CCCWGGG 1 cut(s) 1642
PceI AGGCCT 2 cut(s) 2563, 3040
PciI ACATGT 1 cut(s) 2578
PctI GAATGC 3 cut(s) 1240, 3820, 3886
PdmI GAANNNNTTC 1 cut(s) 2969
PflFI GACNNNGTC 1 cut(s) 3273
PflMI CCANNNNNTGG 9 cut(s) 242, 845, 1772, 2732, 3236, 3308, 3650, 3701, 3847
PfoI TCCNGGA 1 cut(s) 745
PleI GAGTC 6 cut(s) 1402, 1705, 2134, 2164, 3125, 3733
PpsI GAGTC 6 cut(s) 1402, 1705, 2134, 2164, 3125, 3733
PpuMI RGGWCCY 2 cut(s) 3443, 3527
PscI ACATGT 1 cut(s) 2578
PshAI GACNNNNGTC 1 cut(s) 209
PshBI ATTAAT 1 cut(s) 2997
PsiI TTATAA 1 cut(s) 2682
Psp5II RGGWCCY 2 cut(s) 3443, 3527
PspEI GGTNACC 2 cut(s) 3224, 3491
PspFI CCCAGC 3 cut(s) 1191, 1341, 2264
PspPPI RGGWCCY 2 cut(s) 3443, 3527
PsrI GAACNNNNNNTAC 2 cut(s) 1797, 1829
PstI CTGCAG 2 cut(s) 2659, 3154
PstNI CAGNNNCTG 3 cut(s) 542, 3149, 3602
PsuI RGATCY 1 cut(s) 3145
PsyI GACNNNGTC 1 cut(s) 3273
PvuII CAGCTG 2 cut(s) 551, 3155
RseI CAYNNNNRTG 5 cut(s) 345, 1674, 2796, 3210, 3429
SalI GTCGAC 1 cut(s) 1279
SaqAI TTAA 6 cut(s) 1029, 2019, 2432, 2549, 2940, 2997
Sau3AI GATC 8 cut(s) 754, 2335, 3019, 3145, 3367, 3466, 3517, 3733
ScaI AGTACT 5 cut(s) 119, 731, 782, 1779, 2200
SchI GAGTC 6 cut(s) 1402, 1705, 2135, 2165, 3126, 3734
SduI GDGCHC 8 cut(s) 179, 221, 356, 1329, 1380, 2716, 2740, 3840
SfaNI GCATC 8 cut(s) 46, 256, 463, 1752, 2344, 2492, 2657, 3306
SfcI CTRYAG 6 cut(s) 420, 1239, 1998, 2406, 2655, 3150
SfuI TTCGAA 2 cut(s) 653, 2901
SinI GGWCC 3 cut(s) 2723, 3443, 3527
SmaI CCCGGG 1 cut(s) 2920
SmiMI CAYNNNNRTG 5 cut(s) 345, 1674, 2796, 3210, 3429
SmlI CTYRAG 6 cut(s) 666, 948, 1125, 1368, 3023, 3739
SmoI CTYRAG 6 cut(s) 666, 948, 1125, 1368, 3023, 3739
SphI GCATGC 1 cut(s) 1723
SseBI AGGCCT 2 cut(s) 2563, 3040
SspI AATATT 1 cut(s) 1601
SspMI CTAG 7 cut(s) 2130, 2358, 2625, 2745, 3036, 3090, 3722
StuI AGGCCT 2 cut(s) 2563, 3040
StyI CCWWGG 5 cut(s) 2624, 2692, 2791, 3035, 3167
TaiI ACGT 6 cut(s) 213, 1351, 1494, 1639, 3649, 3819
TatI WGTACW 6 cut(s) 117, 729, 780, 1777, 2198, 2416
TauI GCSGC 2 cut(s) 2430, 2824
Tru1I TTAA 6 cut(s) 1029, 2019, 2432, 2549, 2940, 2997
Tru9I TTAA 6 cut(s) 1029, 2019, 2432, 2549, 2940, 2997
TscAI CASTG 5 cut(s) 179, 600, 710, 1329, 2275
TseFI GTSAC 5 cut(s) 168, 253, 595, 3224, 3491
Tsp45I GTSAC 5 cut(s) 168, 253, 595, 3224, 3491
TspMI CCCGGG 1 cut(s) 2918
TspRI CASTG 5 cut(s) 179, 600, 710, 1329, 2275
Tth111I GACNNNGTC 1 cut(s) 3273
Van91I CCANNNNNTGG 9 cut(s) 242, 845, 1772, 2732, 3236, 3308, 3650, 3701, 3847
VneI GTGCAC 2 cut(s) 175, 352
VpaK11BI GGWCC 3 cut(s) 2723, 3443, 3527
VspI ATTAAT 1 cut(s) 2997
XagI CCTNNNNNAGG 3 cut(s) 2508, 2706, 3100
XapI RAATTY 8 cut(s) 412, 655, 1074, 1542, 1906, 1978, 3139, 3383
XbaI TCTAGA 1 cut(s) 3089
XceI RCATGY 5 cut(s) 1723, 2582, 2875, 3569, 3596
XcmI CCANNNNNNNNNTGG 2 cut(s) 626, 743
XmaI CCCGGG 1 cut(s) 2918
XmaJI CCTAGG 2 cut(s) 2624, 3035
XmiI GTMKAC 2 cut(s) 147, 1280
XmnI GAANNNNTTC 1 cut(s) 2969
XspI CTAG 7 cut(s) 2130, 2358, 2625, 2745, 3036, 3090, 3722
ZrmI AGTACT 5 cut(s) 119, 731, 782, 1779, 2200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.