Rmu_sc0000317.1_g000010

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000317.1
OG0004457
Physical Location & Seq
Forward (+)
54415 .. 64130
9716 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2343 bp
atgctgccgaatctgtctccctttggcagcaatttgggcaatccgtttttgttgaatggcgatttgagcagcgagggtgctcctgctcctagcgttttgttcttggtcccgtttctcttgttccaaggaggaaccggaatggatttgtccaaggtcggcgagaaaattctcagctccgtcaggtctgccagatcactcggtcttcttcctcccgcttctgatcgcccggaggttccggcgcgagctgcagctgctgcagttgttgctcgtgctattgcgggactgcctccacatcaaagatatggtctctcttcaagctctgaagaattgagctcaatttatgggagcagacaacaacatggtgaagaagtggaggagatagaggaagaattctatgaagagggttttgatccagttagacatattttggagcatattccttctgacgaaaatgagctggcttattttgagagacaggcagctcttaaattagcacaactggatagagtatcagaacatttatcccgaaatgttatggaacatcatgaagtaatggtgaaggggatgcatttggtcagggaattggagaaagacttaaaagttgcgaatgtcatttgcatgaatggaagacggcatcttacttcatcgagaaatgaggtttctagggatctgatcgtaaattcctattccaagaagaaacatgctcttctggacatggttccagttttgaatgagcttcgtcatgcgttagaaatgcaatccgaactcgagtctcttgttgaggaaggaaattactgcagggcctttcaagtactgtctgagtatctgcagctattagatagtttctcagaactatctgcagtacaagagatgagccgtggagttgaggtttggctgggacaaactcttcagaagctggattccttgttgctaggagtgtgccagaaattcaatgaggagggctatataactgtggttgatgcgtatgctctaattggggacatctctggtcttgctgagaagatacaaagttactttatgcaggaagttctctcagaaacccattctatattgaagaccattgtgcaagaggatcaggaagtagacatgcaaaatagtcgacttacctacagtgatctatgccgtcagatacctgaacctaagtttaggcagtgtttgttaaatacactagcgattctattcaagttgatgtgctcatatcatgaaatcatggcctttcagctggatgataaggattcggccaagaaaacttcaagcattgagccagaggaaagtgacatccctcaaattccaggaggcgttcagaacatttcaacgtctttctcgtccgcaaaagctaatggctctctctcggaatgtgtagatgtaatagcaggttcatattctatggaggaatctcataccaactcttccaccgagcctacgggaaacaccacttccgtgtgtacaacatctcatgatttagttgatgaggctaggatggatggcagtgctgcgtcaacgagtgggtctccttggtatcaactgcggaaagatgccactgcatttgtttcacaaacccttcaaagaggacgcaaaaatctctggcatctcacagctactcgtgtatcagtcttgctttcttctgcttctgtatcttctgccagcattcaccaattcttgaaaaattatgaagatctgagtgccttcatcttggctggggaagcattttgcggaattgaagcagctgacttcaggcagaagttgaaggcagtttgtgaaagttactttttagcttttcatcggcagaatatctttgcgctcaaaatggtcctggaaaaagaactttggttaatcatgccacctgatacggtacaagaaattacctttcctgggcttgttggtgatggagcacctttaattattccatctaatagtaagtccagggtgctactttctgataagtctgctagattggttgatactggtgtcaagaagagtggtttttctatttggataaaaaatggaaacccttttgcattaaaactgccacactcgtccaaagaaggtccgaaagggaatggtacaacttctggtgaatttgatggaaattttagtgaaggtgacaaagtctctccacgaaagagtgatgccaatcattcgaatggtgctaattctgtttcggaagacgaaaatgaggaccttcttgcagactttattgatgaggatagtcaactccccagtcgaatttcaaaaccgaaacacccgagaagccaatcttcacactggaatgctggagagctaatagcccaaacaggatcgtctatttgccttctaaggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2451 Vps54_N

Protein Analysis

780

Amino Acids

85.91

Weight (kDa)

5.18

Isoelectric Point (pI)

48.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps54_N PF10475 124 - 413 5.3e-97 Vacuolar-sorting protein 54, of GARP complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1385
AccI GTMKAC 2 cut(s) 1104, 1120
AccII CGCG 1 cut(s) 241
AciI CCGC 5 cut(s) 213, 278, 1350, 1549, 1734
AclWI GGATC 4 cut(s) 404, 675, 1101, 2326
AcoI YGGCCR 1 cut(s) 1260
AcsI RAATTY 8 cut(s) 165, 389, 679, 947, 1308, 2099, 2110, 2247
AcuI CTGAAG 3 cut(s) 342, 893, 1738
AfaI GTAC 5 cut(s) 813, 864, 1468, 1875, 2086
AfiI CCNNNNNNNGG 1 cut(s) 1893
AjnI CCWGG 4 cut(s) 1312, 1833, 1891, 1943
AleI CACNNNNGTG 1 cut(s) 1460
Alw21I GWGCWC 4 cut(s) 82, 335, 1217, 1915
Alw26I GTCTC 6 cut(s) 21, 311, 466, 777, 1536, 2137
AlwI GGATC 4 cut(s) 404, 675, 1101, 2326
AlwNI CAGNNNCTG 2 cut(s) 254, 916
Ama87I CYCGRG 2 cut(s) 767, 2266
AoxI GGCC 3 cut(s) 801, 1233, 1260
ApoI RAATTY 8 cut(s) 165, 389, 679, 947, 1308, 2099, 2110, 2247
ArsI GACNNNNNNTTYG 2 cut(s) 289, 321
AspLEI GCGC 2 cut(s) 241, 1822
AspS9I GGNCC 5 cut(s) 106, 801, 1831, 2069, 2200
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 6 cut(s) 374, 568, 1664, 1916, 2108, 2135
AsuII TTCGAA 1 cut(s) 2162
AvaI CYCGRG 2 cut(s) 767, 2266
AvaII GGWCC 4 cut(s) 106, 1831, 2069, 2200
BaeI ACNNNNGTAYC 4 cut(s) 1611, 1644, 1974, 2007
BanII GRGCYC 1 cut(s) 335
BauI CACGAG 2 cut(s) 267, 1623
BbsI GAAGAC 4 cut(s) 194, 634, 1082, 2193
Bbv12I GWGCWC 4 cut(s) 82, 335, 1217, 1915
BccI CCATC 5 cut(s) 1495, 1499, 1901, 1936, 2099
BceAI ACGGC 3 cut(s) 647, 861, 1128
BcgI CGANNNNNNTGC 2 cut(s) 1100, 1134
BciT130I CCWGG 4 cut(s) 1314, 1835, 1893, 1945
BcnI CCSGG 1 cut(s) 227
BcoDI GTCTC 6 cut(s) 21, 311, 466, 777, 1536, 2137
BfaI CTAG 6 cut(s) 90, 663, 932, 1190, 1497, 1971
BfmI CTRYAG 6 cut(s) 246, 255, 796, 827, 858, 1129
BfuAI ACCTGC 1 cut(s) 1385
BglII AGATCT 1 cut(s) 1696
BmcAI AGTACT 1 cut(s) 813
Bme1390I CCNGG 5 cut(s) 227, 1314, 1835, 1893, 1945
Bme18I GGWCC 4 cut(s) 106, 1831, 2069, 2200
BmeT110I CYCGRG 2 cut(s) 767, 2266
BmgT120I GGNCC 5 cut(s) 106, 801, 1831, 2069, 2200
BmiI GGNNCC 4 cut(s) 108, 133, 234, 720
BmrFI CCNGG 5 cut(s) 227, 1314, 1835, 1893, 1945
BmrI ACTGGG 1 cut(s) 2235
BmsI GCATC 6 cut(s) 555, 643, 970, 1546, 1618, 2140
BmuI ACTGGG 1 cut(s) 2235
BpiI GAAGAC 4 cut(s) 194, 634, 1082, 2193
BplI GAGNNNNNCTC 2 cut(s) 1516, 1548
BpmI CTGGAG 1 cut(s) 2316
Bpu14I TTCGAA 1 cut(s) 2162
BpuMI CCSGG 1 cut(s) 227
BsaBI GATNNNNATC 1 cut(s) 2154
BsaI GGTCTC 2 cut(s) 311, 1536
BsaJI CCNNGG 6 cut(s) 124, 150, 877, 1535, 1892, 1944
BsaWI WCCGGW 1 cut(s) 134
BsaXI ACNNNNNCTCC 2 cut(s) 873, 903
Bsc4I CCNNNNNNNGG 1 cut(s) 1893
Bse1I ACTGG 6 cut(s) 413, 504, 722, 1990, 2241, 2291
Bse8I GATNNNNATC 1 cut(s) 2154
BseBI CCWGG 4 cut(s) 1314, 1835, 1893, 1945
BseDI CCNNGG 6 cut(s) 124, 150, 877, 1535, 1892, 1944
BseGI GGATG 5 cut(s) 570, 1252, 1299, 1506, 1510
BseJI GATNNNNATC 1 cut(s) 2154
BseLI CCNNNNNNNGG 1 cut(s) 1893
BseMII CTCAG 6 cut(s) 184, 810, 861, 1008, 1068, 1691
BseNI ACTGG 6 cut(s) 413, 504, 722, 1990, 2241, 2291
BseRI GAGGAG 2 cut(s) 389, 971
BseYI CCCAGC 2 cut(s) 895, 1718
Bsh1236I CGCG 1 cut(s) 241
BshFI GGCC 3 cut(s) 803, 1235, 1262
BsiHKAI GWGCWC 4 cut(s) 82, 335, 1217, 1915
BsiHKCI CYCGRG 2 cut(s) 767, 2266
BsiSI CCGG 3 cut(s) 135, 227, 236
BslFI GGGAC 4 cut(s) 92, 294, 912, 1013
BslI CCNNNNNNNGG 1 cut(s) 1893
BsmAI GTCTC 6 cut(s) 21, 311, 466, 777, 1536, 2137
BsmFI GGGAC 4 cut(s) 92, 294, 912, 1013
BsmI GAATGC 2 cut(s) 1668, 2296
BsnI GGCC 3 cut(s) 803, 1235, 1262
Bso31I GGTCTC 2 cut(s) 311, 1536
BsoBI CYCGRG 2 cut(s) 767, 2266
Bsp119I TTCGAA 1 cut(s) 2162
Bsp1286I GDGCHC 4 cut(s) 82, 335, 1217, 1915
Bsp1407I TGTACA 1 cut(s) 1466
Bsp143I GATC 9 cut(s) 191, 220, 409, 667, 672, 1093, 1135, 1696, 2318
BspACI CCGC 5 cut(s) 213, 278, 1350, 1549, 1734
BspANI GGCC 3 cut(s) 803, 1235, 1262
BspCNI CTCAG 6 cut(s) 183, 811, 860, 1009, 1067, 1692
BspFNI CGCG 1 cut(s) 241
BspHI TCATGA 3 cut(s) 544, 1222, 1477
BspLI GGNNCC 4 cut(s) 108, 133, 234, 720
BspMAI CTGCAG 5 cut(s) 250, 259, 800, 831, 862
BspMI ACCTGC 1 cut(s) 1385
BspPI GGATC 4 cut(s) 404, 675, 1101, 2326
BspQI GCTCTTC 1 cut(s) 711
BspT104I TTCGAA 1 cut(s) 2162
BspTNI GGTCTC 2 cut(s) 311, 1536
BsrGI TGTACA 1 cut(s) 1466
BsrI ACTGG 6 cut(s) 413, 504, 722, 1990, 2241, 2291
BssECI CCNNGG 6 cut(s) 124, 150, 877, 1535, 1892, 1944
BssMI GATC 9 cut(s) 191, 220, 409, 667, 672, 1093, 1135, 1696, 2318
BssSI CACGAG 2 cut(s) 267, 1623
BssT1I CCWWGG 3 cut(s) 124, 150, 1535
Bst2BI CACGAG 2 cut(s) 267, 1623
Bst2UI CCWGG 4 cut(s) 1314, 1835, 1893, 1945
Bst4CI ACNGT 4 cut(s) 816, 973, 1133, 1873
Bst6I CTCTTC 6 cut(s) 316, 393, 711, 912, 1435, 1991
BstAPI GCANNNNNTGC 2 cut(s) 254, 263
BstAUI TGTACA 1 cut(s) 1466
BstBI TTCGAA 1 cut(s) 2162
BstC8I GCNNGC 3 cut(s) 243, 459, 1666
BstDEI CTNAG 8 cut(s) 170, 819, 847, 1017, 1054, 1161, 1700, 2336
BstDSI CCRYGG 1 cut(s) 877
BstF5I GGATG 5 cut(s) 570, 1252, 1299, 1506, 1510
BstFNI CGCG 1 cut(s) 241
BstHHI GCGC 2 cut(s) 241, 1822
BstKTI GATC 9 cut(s) 194, 223, 412, 670, 675, 1096, 1138, 1699, 2321
BstMAI GTCTC 6 cut(s) 21, 311, 466, 777, 1536, 2137
BstMBI GATC 9 cut(s) 191, 220, 409, 667, 672, 1093, 1135, 1696, 2318
BstMWI GCNNNNNNNGC 7 cut(s) 36, 66, 245, 251, 254, 263, 1724
BstNI CCWGG 4 cut(s) 1314, 1835, 1893, 1945
BstNSI RCATGY 2 cut(s) 704, 1111
BstSCI CCNGG 5 cut(s) 225, 1312, 1833, 1891, 1943
BstSFI CTRYAG 6 cut(s) 246, 255, 796, 827, 858, 1129
BstUI CGCG 1 cut(s) 241
BstV2I GAAGAC 4 cut(s) 194, 634, 1082, 2193
BstX2I RGATCY 2 cut(s) 667, 1696
BstYI RGATCY 2 cut(s) 667, 1696
BsuRI GGCC 3 cut(s) 803, 1235, 1262
BtgI CCRYGG 1 cut(s) 877
BtsCI GGATG 5 cut(s) 570, 1252, 1299, 1506, 1510
BtsI GCAGTG 3 cut(s) 1178, 1516, 1560
BtsIMutI CAGTG 5 cut(s) 1138, 1178, 1516, 1560, 2284
BveI ACCTGC 1 cut(s) 1385
Cac8I GCNNGC 3 cut(s) 243, 459, 1666
CaiI CAGNNNCTG 2 cut(s) 254, 916
CciI TCATGA 3 cut(s) 544, 1222, 1477
CfoI GCGC 2 cut(s) 241, 1822
Cfr13I GGNCC 5 cut(s) 106, 801, 1831, 2069, 2200
CseI GACGC 2 cut(s) 1506, 1602
Csp6I GTAC 5 cut(s) 812, 863, 1467, 1874, 2085
CspCI CAANNNNNGTGG 4 cut(s) 1549, 1584, 1981, 2016
CviQI GTAC 5 cut(s) 812, 863, 1467, 1874, 2085
DdeI CTNAG 8 cut(s) 170, 819, 847, 1017, 1054, 1161, 1700, 2336
DpnI GATC 9 cut(s) 193, 222, 411, 669, 674, 1095, 1137, 1698, 2320
DpnII GATC 9 cut(s) 191, 220, 409, 667, 672, 1093, 1135, 1696, 2318
EaeI YGGCCR 1 cut(s) 1260
Eam1104I CTCTTC 6 cut(s) 316, 393, 711, 912, 1435, 1991
EarI CTCTTC 6 cut(s) 316, 393, 711, 912, 1435, 1991
Ecl136II GAGCTC 1 cut(s) 333
Eco130I CCWWGG 3 cut(s) 124, 150, 1535
Eco24I GRGCYC 1 cut(s) 335
Eco31I GGTCTC 2 cut(s) 311, 1536
Eco47I GGWCC 4 cut(s) 106, 1831, 2069, 2200
Eco53kI GAGCTC 1 cut(s) 333
Eco57I CTGAAG 3 cut(s) 342, 893, 1738
Eco88I CYCGRG 2 cut(s) 767, 2266
EcoICRI GAGCTC 1 cut(s) 333
EcoO109I RGGNCCY 2 cut(s) 801, 2200
EcoRI GAATTC 1 cut(s) 389
EcoRII CCWGG 4 cut(s) 1312, 1833, 1891, 1943
EcoT14I CCWWGG 3 cut(s) 124, 150, 1535
EcoT22I ATGCAT 1 cut(s) 570
EcoT38I GRGCYC 1 cut(s) 335
ErhI CCWWGG 3 cut(s) 124, 150, 1535
FalI AAGNNNNNCTT 2 cut(s) 2263, 2295
FaqI GGGAC 4 cut(s) 92, 294, 912, 1013
FauI CCCGC 2 cut(s) 220, 271
FblI GTMKAC 2 cut(s) 1104, 1120
FokI GGATG 5 cut(s) 577, 1259, 1286, 1513, 1517
FriOI GRGCYC 1 cut(s) 335
FspBI CTAG 6 cut(s) 90, 663, 932, 1190, 1497, 1971
GlaI GCGC 2 cut(s) 240, 1821
GsaI CCCAGC 2 cut(s) 899, 1722
GsuI CTGGAG 1 cut(s) 2316
HaeIII GGCC 3 cut(s) 803, 1235, 1262
HapII CCGG 3 cut(s) 135, 227, 236
HgaI GACGC 2 cut(s) 1506, 1602
HhaI GCGC 2 cut(s) 241, 1822
Hin6I GCGC 2 cut(s) 239, 1820
HinP1I GCGC 2 cut(s) 239, 1820
HincII GTYRAC 3 cut(s) 1121, 1521, 2234
HindII GTYRAC 3 cut(s) 1121, 1521, 2234
HinfI GANTC 6 cut(s) 10, 770, 920, 1195, 1256, 1415
HpaII CCGG 3 cut(s) 135, 227, 236
HphI GGTGA 6 cut(s) 374, 568, 1664, 1916, 2108, 2135
Hpy166II GTNNAC 5 cut(s) 1105, 1121, 1467, 1521, 2234
Hpy188III TCNNGA 9 cut(s) 525, 545, 648, 710, 1097, 1223, 1478, 1681, 1993
Hpy8I GTNNAC 5 cut(s) 1105, 1121, 1467, 1521, 2234
HpyCH4III ACNGT 4 cut(s) 816, 973, 1133, 1873
HpyCH4IV ACGT 1 cut(s) 1337
HpyF10VI GCNNNNNNNGC 7 cut(s) 36, 66, 245, 251, 254, 263, 1724
HpyF3I CTNAG 8 cut(s) 170, 819, 847, 1017, 1054, 1161, 1700, 2336
HpySE526I ACGT 1 cut(s) 1337
HspAI GCGC 2 cut(s) 239, 1820
Kzo9I GATC 9 cut(s) 191, 220, 409, 667, 672, 1093, 1135, 1696, 2318
LguI GCTCTTC 1 cut(s) 711
LmnI GCTCC 6 cut(s) 85, 91, 179, 345, 430, 1910
LweI GCATC 6 cut(s) 555, 643, 970, 1546, 1618, 2140
MaeI CTAG 6 cut(s) 90, 663, 932, 1190, 1497, 1971
MaeII ACGT 1 cut(s) 1337
MaeIII GTNAC 4 cut(s) 1031, 1295, 1784, 2123
MalI GATC 9 cut(s) 193, 222, 411, 669, 674, 1095, 1137, 1698, 2320
MboI GATC 9 cut(s) 191, 220, 409, 667, 672, 1093, 1135, 1696, 2318
MflI RGATCY 2 cut(s) 667, 1696
MhlI GDGCHC 4 cut(s) 82, 335, 1217, 1915
MlyI GAGTC 1 cut(s) 779
Mph1103I ATGCAT 1 cut(s) 570
MseI TTAA 6 cut(s) 486, 596, 1181, 1853, 1919, 2042
MslI CAYNNNNRTG 4 cut(s) 617, 1460, 2163, 2289
MspA1I CMGCKG 3 cut(s) 251, 1243, 1748
MspI CCGG 3 cut(s) 135, 227, 236
MspR9I CCNGG 5 cut(s) 227, 1314, 1835, 1893, 1945
Mva1269I GAATGC 2 cut(s) 1668, 2296
MvaI CCWGG 4 cut(s) 1314, 1835, 1893, 1945
MvnI CGCG 1 cut(s) 241
MwoI GCNNNNNNNGC 7 cut(s) 36, 66, 245, 251, 254, 263, 1724
NciI CCSGG 1 cut(s) 227
NdeII GATC 9 cut(s) 191, 220, 409, 667, 672, 1093, 1135, 1696, 2318
NlaIV GGNNCC 4 cut(s) 108, 133, 234, 720
NmuCI GTSAC 2 cut(s) 1295, 2123
NsiI ATGCAT 1 cut(s) 570
NspI RCATGY 2 cut(s) 704, 1111
NspV TTCGAA 1 cut(s) 2162
OliI CACNNNNGTG 1 cut(s) 1460
PaeR7I CTCGAG 1 cut(s) 767
PagI TCATGA 3 cut(s) 544, 1222, 1477
PciSI GCTCTTC 1 cut(s) 711
PcsI WCGNNNNNNNCGW 1 cut(s) 1343
PctI GAATGC 2 cut(s) 1668, 2296
PfeI GAWTC 5 cut(s) 10, 920, 1195, 1256, 1415
PflFI GACNNNGTC 1 cut(s) 2129
PfoI TCCNGGA 2 cut(s) 1312, 1833
PleI GAGTC 1 cut(s) 778
PpsI GAGTC 1 cut(s) 778
PpuMI RGGWCCY 1 cut(s) 2200
Psp124BI GAGCTC 1 cut(s) 335
Psp5II RGGWCCY 1 cut(s) 2200
Psp6I CCWGG 4 cut(s) 1312, 1833, 1891, 1943
PspFI CCCAGC 2 cut(s) 895, 1718
PspGI CCWGG 4 cut(s) 1312, 1833, 1891, 1943
PspN4I GGNNCC 4 cut(s) 108, 133, 234, 720
PspPI GGNCC 5 cut(s) 106, 801, 1831, 2069, 2200
PspPPI RGGWCCY 1 cut(s) 2200
PspXI VCTCGAGB 1 cut(s) 767
PstI CTGCAG 5 cut(s) 250, 259, 800, 831, 862
PstNI CAGNNNCTG 2 cut(s) 254, 916
PsuI RGATCY 2 cut(s) 667, 1696
PsyI GACNNNGTC 1 cut(s) 2129
PvuII CAGCTG 3 cut(s) 251, 1243, 1748
RsaI GTAC 5 cut(s) 813, 864, 1468, 1875, 2086
RsaNI GTAC 5 cut(s) 812, 863, 1467, 1874, 2085
RseI CAYNNNNRTG 4 cut(s) 617, 1460, 2163, 2289
SacI GAGCTC 1 cut(s) 335
SalI GTCGAC 1 cut(s) 1119
SapI GCTCTTC 1 cut(s) 711
SaqAI TTAA 6 cut(s) 486, 596, 1181, 1853, 1919, 2042
Sau3AI GATC 9 cut(s) 191, 220, 409, 667, 672, 1093, 1135, 1696, 2318
Sau96I GGNCC 5 cut(s) 106, 801, 1831, 2069, 2200
ScaI AGTACT 1 cut(s) 813
SchI GAGTC 1 cut(s) 779
ScrFI CCNGG 5 cut(s) 227, 1314, 1835, 1893, 1945
SduI GDGCHC 4 cut(s) 82, 335, 1217, 1915
SfaNI GCATC 6 cut(s) 555, 643, 970, 1546, 1618, 2140
SfcI CTRYAG 6 cut(s) 246, 255, 796, 827, 858, 1129
Sfr274I CTCGAG 1 cut(s) 767
SfuI TTCGAA 1 cut(s) 2162
SinI GGWCC 4 cut(s) 106, 1831, 2069, 2200
SlaI CTCGAG 1 cut(s) 767
SmiMI CAYNNNNRTG 4 cut(s) 617, 1460, 2163, 2289
SmlI CTYRAG 1 cut(s) 767
SmoI CTYRAG 1 cut(s) 767
SsiI CCGC 5 cut(s) 213, 278, 1350, 1549, 1734
SspMI CTAG 6 cut(s) 90, 663, 932, 1190, 1497, 1971
SstI GAGCTC 1 cut(s) 335
StyD4I CCNGG 5 cut(s) 225, 1312, 1833, 1891, 1943
StyI CCWWGG 3 cut(s) 124, 150, 1535
TaaI ACNGT 4 cut(s) 816, 973, 1133, 1873
TaiI ACGT 1 cut(s) 1340
TaqI TCGA 5 cut(s) 647, 768, 1120, 2162, 2245
TaqII GACCGA 1 cut(s) 188
TatI WGTACW 3 cut(s) 811, 862, 1466
TfiI GAWTC 5 cut(s) 10, 920, 1195, 1256, 1415
Tru1I TTAA 6 cut(s) 486, 596, 1181, 1853, 1919, 2042
Tru9I TTAA 6 cut(s) 486, 596, 1181, 1853, 1919, 2042
TscAI CASTG 5 cut(s) 1138, 1178, 1516, 1567, 2291
TseFI GTSAC 2 cut(s) 1295, 2123
Tsp45I GTSAC 2 cut(s) 1295, 2123
TspDTI ATGAA 9 cut(s) 411, 561, 633, 635, 1239, 1389, 1699, 1707, 1790
TspGWI ACGGA 3 cut(s) 33, 166, 1450
TspRI CASTG 5 cut(s) 1138, 1178, 1516, 1567, 2291
Tth111I GACNNNGTC 1 cut(s) 2129
VpaK11BI GGWCC 4 cut(s) 106, 1831, 2069, 2200
XapI RAATTY 8 cut(s) 165, 389, 679, 947, 1308, 2099, 2110, 2247
XceI RCATGY 2 cut(s) 704, 1111
XhoI CTCGAG 1 cut(s) 767
XmiI GTMKAC 2 cut(s) 1104, 1120
XspI CTAG 6 cut(s) 90, 663, 932, 1190, 1497, 1971
ZrmI AGTACT 1 cut(s) 813
Zsp2I ATGCAT 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.