Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000435.1

Orthogroup ID

OG0005110

Physical Location & Seq

Genomic Coordinates Forward (+)

58385 .. 70822

Gene Structure 12438 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3861 bp

atggatccgatacaagcagccaaggagctctcattccgggtcggcttctccggccacagcggccacctccgcctcgagcctctctgcaccgtcgagcgctccgaccccgtcaagtcgctccccgatttcgtcctgccgcctgcatttgctagggaaacgccggagtcgataaaagagtatatagaggagacatatctgttgccgagattggattctgatgtcttttcaccggaaaaggttgggaggcagtgggattttgactggtttgacaaggctaatgtacacttggagccgtcgttgccacggtctgttgtcgttccgacatgggagttaccgtttaggcgccagaagaatggatcagaaggagggatatgggaacccaaatctgtgcaggtcgatgaagcagaacttaccatagaagcacaagagtctggttcgttgccacgcatggccggaccagcaaaggattttgtatggggaagcatcagcaaccgtccttttcgtccaggaggcttggatgattcccaatctctagaaagaactcttcctgatggtgcttctaatggcgaatgggtacaccaacttcttactggtggccctgcacaggctgttccaccaagctttaagcaaggattggaccttggtcctctcaaggcatatcctggctcatggaatgtatacaatgatcaaggttcagtcaagagcacatcagatgaaaagctgggtttgcagagtgagttgtctgtacagtttgatgacttgttcaaaaaggcttgggatgaggatgttgttgagtttgaaggagatggatcggaatctgcaaagtcagaggatgaagcaaatgtggttgatgttgatattaccagcaatcctcctgagcctgaactgtctgtgttggatgagattttgtcagttgaggcaggagactcaaagtcaagatttaatggaactggtggtgaacagaacccggaggcttgggctattagtggacgtactgaatggatttcggagaattttcatgatcttgttcctgataaggcacttgattttccctttgaattggatacattccaaaaggaggcaatttattatcttgagaagggggagtctgtctttgtatctgctcacacatcagctggaaagacagtagttgcagaatatgcatttgctttggcatcaaaacattgcacgagagctgtgtatactgctcctatcaagaccatcagcaaccaaaaatatagggacttctgtggaaaattcgatgttggacttctcactggtgatgttagcttgaggcccgaggcttcttgtctcattatgaccacggaaatattaaggtcaatgctttatcggggtgctgacatcatacgtgacattgaatgggttatctttgatgaggttcattatgtcaatgatgtcgaaagaggtgttgtttgggaagaagttattataatgctcccgagacacatcaacattgtcctcctttcagctacggtaccgaacaaagttgagtttgctgactggattggacggacaaagcaaaagcaaatccgtgttactggggcttcaaagctgcaaaagatacattcaagaaaaagaatagtcctgccaccggcggcggcggcggtggatcacgtgctccagcttctgcttcagcttctcacgatggggctcgaggtcaaaaacgtgaaacttcacattcaggaaaacagaagcaattgggtgcccataactcagggaatttatccagaactggtggttatattttgtttctcaaagaatcgctgtgataggtcagctgatagcatgtcgggaattgacctcacaagtagttctgagaaaagtcagattcgtgttttctgtgacaaagcattttcaaggctgaagggatccgacaggaatttacctcaggttgtcagagttcaaaaccttctccatagagggattggtgtccatcatgcaggacttcttccaattgttaaggaagttgttgaaatgctcttttgtcgaggtgtgataaaggttctgttttcaacagagacatttgcaatgggagtgaatgcaccagctagaacggttgtctttgatacattaaggaaatttgatggcaaggaatttagacaactgctaccaggggaatacactcaaatggcaggccgtgctggcaggagaggacttgataaaattggtacagttattgtaatgtgccgtgatgaaatcctaggagagggggatttgaagcatgtcatagttggaagtgcaaccaggctagaatctcagtttcggcttacttatattatgatcatgcatcttcttcgtgttgaagaactgaaggtggaggacatgctgaaaagaagttttgctgagttccatgctcagaagaaactaccagatatgcagcagcttctgatgcgaaagcttgctcagcctacaaaaagtatcgaatgtataaaaggggaaccagctattgaggagtattatgacatgcacttagaaaaggagaagcatagaacagaagtattggaggcagtaatgcagtcccctggtgcccaacaatttcttacatccggaagagtggttgtcatgaaatcacaatcacaggcccagaaatacttgcttggagttgttgtaaaagcaccttcttctagtaataagcaacacatcgttctagtgctgaaacctgaactaccagcgacaatccaaactcccctggctagtggtaacttacaagataccaaaaacactgattcttctcagggttactacatggtagcgaaatctaaacgtgcttttgaagaagagtattgtacctctgtttcttcacgcaaaggatcaggtgtcatcaacataaaattaccacaccatggtgctgctgctggggtgagatatgaggttagaggagccgatatcacagacttctcattcatatgcagtagcaaaataaagattgacctagtccggcttcttgaggactgtagcagtgctgcttactccaagacagtgcaactgctgttggacacaaaacctaaaggagaggagtaccctctagccttagatccagtgaaagatctgaagttgaaagacatgcatcttgtggaaacatactacaaatggacaaacttagtgcagaagatggcaaagaataagtgcgatggatgtattaaattggaggaacacattcatttagcaagagagataaagagacacaaagaggaagttaatgctcttaagtttcaaatgtcagatgaagcacttcaacagatgccagattttcaaggccggatagatgttctgaaggaaatcggatgtatagatgctgaccttgtagttcaaataaaaggccgtgtcgcatgcgagatgaattctggggaggagttgatctgcacagagtgtttgtttgagaaccaactagatgacctggaaccagaagaagcggtggcattaatgtcgtccttcgtttttcagcagaagaatacttctgaaccttcacttactcctaaactttctatggcgaaagagagattgtacaatacagcaataagactaggcaagtgtcaagcttacttcaaacttccaataaatccagaagagtacgccaaagagaatctcaagtttggtcttgttgaagtcgtttatgagtgggcaaagggcacaccatttgcagatatttgtgaactcacagatgttcctgaaggcatgatagtccggaccattgttagacttgatgagacgtgtcgtgaattcaaaaatgctgcatctattatgggtaattctgctctatacaagaaaatggagacagcttcaaatgcaataaaacgtgacattgttttcgcagctagcttgtacgtaacaggagtataa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000184 GO:0000288 GO:0000291 GO:0000956 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0015672 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019439 GO:0030001 GO:0032991 GO:0034427 GO:0034641 GO:0034655 GO:0035864 GO:0040029 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0050789 GO:0050896 GO:0051179 GO:0051234 GO:0055087 GO:0060255 GO:0065007 GO:0070035 GO:0070478 GO:0071704 GO:0090304 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

1286

Amino Acids

144.16

Weight (kDa)

5.67

Isoelectric Point (pI)

43.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ski2_N	PF17911	114 - 215	4.4e-09	Ski2 N-terminal region
DEAD	PF00270	357 - 504	4.8e-23	DEAD/DEAH box helicase
Helicase_C	PF00271	612 - 725	5.5e-06	Helicase conserved C-terminal domain
MTR4-like_stalk	PF21408	780 - 844	9e-13	Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel	PF13234	848 - 1082	5.5e-24	Mtr4-like, beta-barrel domain
DSHCT	PF08148	1108 - 1278	1.5e-58	DSHCT (NUC185) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46960 AT3G46960 AT3G46960
fragaria_vesca	FvH4_3g37880 FvH4_3g37880 FvH4_3g37880 FvH4_3g37880 FvH4_3g37880
malus_domestica	MD03G1077900.v1.1 MD11G1082500.v1.1
prunus_persica	Prupe.6G062900_v2.0.a1 Prupe.6G062900_v2.0.a1 Prupe.6G062900_v2.0.a1
pyrus_communis	pycom03g06210 pycom11g07060
rosa_chinensis	RchiOBHm_Chr5g0068091
rosa_laevigata	RLG00000035954
rosa_multiflora	Rmu_sc0000435.1_g000017
rosa_roxburghii	Rroxscaffold_1G00012520 Rroxscaffold_1G00013260
rosa_rugosa	Rorug05G0387900 Rorug05G0388300 Rorug05G0388400 Rorug05G0388500
rosa_samantha	Rh5AG446500 Rh5BG464600 Rh5CG486400 Rh5DG478400
rosa_wichuraiana	Rw5G041700 Rw5G041740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1451
Acc36I	ACCTGC	1 cut(s)	382
Acc65I	GGTACC	1 cut(s)	1495
AccB1I	GGYRCC	4 cut(s)	342, 1495, 1723, 2546
AccB7I	CCANNNNNTGG	1 cut(s)	2864
AccI	GTMKAC	2 cut(s)	678, 1202
AccIII	TCCGGA	2 cut(s)	2567, 3705
AciI	CCGC	8 cut(s)	60, 70, 137, 1616, 1619, 1622, 1625, 3434
AclWI	GGATC	8 cut(s)	12, 364, 817, 1638, 1884, 1897, 2839, 3050
AcoI	YGGCCR	3 cut(s)	52, 61, 450
AcsI	RAATTY	8 cut(s)	1012, 1256, 1740, 1900, 2099, 2114, 3361, 3740
AcuI	CTGAAG	6 cut(s)	1637, 1904, 2351, 3092, 3314, 3711
AcvI	CACGTG	1 cut(s)	1636
AcyI	GRCGYC	1 cut(s)	343
AdeI	CACNNNGTG	1 cut(s)	3390
AfeI	AGCGCT	1 cut(s)	98
AfiI	CCNNNNNNNGG	5 cut(s)	604, 1078, 1612, 2227, 2864
AflII	CTTAAG	1 cut(s)	3227
AflIII	ACRYGT	1 cut(s)	3731
AhdI	GACNNNNNGTC	1 cut(s)	931
AjiI	CACGTC	1 cut(s)	3732
AjnI	CCWGG	7 cut(s)	505, 661, 2131, 2264, 2542, 2709, 3417
AleI	CACNNNNGTG	1 cut(s)	2865
Alw21I	GWGCWC	3 cut(s)	30, 707, 1641
Alw26I	GTCTC	8 cut(s)	182, 918, 1316, 1456, 2033, 3196, 3722, 3788
AlwI	GGATC	8 cut(s)	12, 364, 817, 1638, 1884, 1897, 2839, 3050
AlwNI	CAGNNNCTG	2 cut(s)	1648, 2407
Ama87I	CYCGRG	4 cut(s)	74, 1298, 1459, 1673
Aor13HI	TCCGGA	2 cut(s)	2567, 3705
Aor51HI	AGCGCT	1 cut(s)	98
AoxI	GGCC	9 cut(s)	52, 61, 450, 595, 1295, 2155, 2601, 3277, 3340
ApeKI	GCWGC	9 cut(s)	17, 1573, 2398, 2401, 2870, 2873, 2984, 3752, 3833
ApoI	RAATTY	8 cut(s)	1012, 1256, 1740, 1900, 2099, 2114, 3361, 3740
ArsI	GACNNNNNNTTYG	2 cut(s)	3812, 3844
AseI	ATTAAT	1 cut(s)	3443
Asp700I	GAANNNNTTC	2 cut(s)	3177, 3252
Asp718I	GGTACC	1 cut(s)	1495
AspA2I	CCTAGG	1 cut(s)	2221
AspLEI	GCGC	2 cut(s)	99, 345
AspS9I	GGNCC	7 cut(s)	455, 596, 637, 644, 1296, 2602, 3708
AsuC2I	CCSGG	2 cut(s)	38, 968
AsuHPI	GGTGA	4 cut(s)	219, 968, 1292, 2893
AsuNHI	GCTAGC	1 cut(s)	3836
AvaI	CYCGRG	4 cut(s)	74, 1298, 1459, 1673
AvaII	GGWCC	4 cut(s)	455, 637, 644, 3708
AvrII	CCTAGG	1 cut(s)	2221
AxyI	CCTNAGG	1 cut(s)	1908
BaeGI	GKGCMC	3 cut(s)	1728, 2551, 3653
BamHI	GGATCC	2 cut(s)	4, 1889
BanI	GGYRCC	4 cut(s)	342, 1495, 1723, 2546
BanII	GRGCYC	2 cut(s)	30, 1674
BarI	GAAGNNNNNNTAC	2 cut(s)	567, 599
BauI	CACGAG	1 cut(s)	1189
BbrPI	CACGTG	1 cut(s)	1636
Bbv12I	GWGCWC	3 cut(s)	30, 707, 1641
BbvI	GCAGC	9 cut(s)	29, 1560, 2410, 2413, 2857, 2860, 2971, 3739, 3845
BccI	CCATC	8 cut(s)	545, 800, 1229, 1660, 1962, 2099, 3127, 3146
BceAI	ACGGC	4 cut(s)	277, 2142, 2193, 3327
BcgI	CGANNNNNNTGC	4 cut(s)	2297, 2331, 3429, 3463
BciT130I	CCWGG	7 cut(s)	507, 663, 2133, 2266, 2544, 2711, 3419
BciVI	GTATCC	1 cut(s)	1057
BclI	TGATCA	2 cut(s)	685, 2301
BcnI	CCSGG	2 cut(s)	38, 968
BcoDI	GTCTC	8 cut(s)	182, 918, 1316, 1456, 2033, 3196, 3722, 3788
BfmI	CTRYAG	1 cut(s)	2974
BfoI	RGCGCY	2 cut(s)	100, 346
BfrI	CTTAAG	1 cut(s)	3227
BfuAI	ACCTGC	1 cut(s)	382
BfuI	GTATCC	1 cut(s)	1057
BglI	GCCNNNNNGGC	2 cut(s)	60, 2163
BglII	AGATCT	1 cut(s)	3067
BlnI	CCTAGG	1 cut(s)	2221
BlpI	GCTNAGC	1 cut(s)	2424
Bme1390I	CCNGG	9 cut(s)	38, 507, 663, 968, 2133, 2266, 2544, 2711, 3419
Bme18I	GGWCC	4 cut(s)	455, 637, 644, 3708
BmeRI	GACNNNNNGTC	1 cut(s)	931
BmeT110I	CYCGRG	4 cut(s)	74, 1298, 1459, 1673
BmgBI	CACGTC	1 cut(s)	3732
BmgT120I	GGNCC	7 cut(s)	455, 596, 637, 644, 1296, 2602, 3708
BmrFI	CCNGG	9 cut(s)	38, 507, 663, 968, 2133, 2266, 2544, 2711, 3419
BmrI	ACTGGG	1 cut(s)	1569
BmsI	GCATC	8 cut(s)	492, 1184, 2317, 2400, 3097, 3252, 3304, 3764
BmtI	GCTAGC	1 cut(s)	3840
BmuI	ACTGGG	1 cut(s)	1569
BoxI	GACNNNNGTC	1 cut(s)	642
BpmI	CTGGAG	1 cut(s)	1625
Bpu10I	CCTNAGC	1 cut(s)	876
Bpu1102I	GCTNAGC	1 cut(s)	2424
BpuEI	CTTGAG	5 cut(s)	635, 1115, 1312, 2987, 3593
BpuMI	CCSGG	2 cut(s)	38, 968
BsaAI	YACGTR	3 cut(s)	1370, 1636, 3847
BsaBI	GATNNNNATC	1 cut(s)	814
BsaHI	GRCGYC	1 cut(s)	343
BsaWI	WCCGGW	3 cut(s)	229, 2567, 3705
BsaXI	ACNNNNNCTCC	6 cut(s)	281, 311, 320, 350, 2613, 2643
Bsc4I	CCNNNNNNNGG	5 cut(s)	604, 1078, 1612, 2227, 2864
Bse118I	RCCGGY	1 cut(s)	1612
Bse1I	ACTGG	8 cut(s)	266, 595, 955, 1282, 1526, 1564, 1758, 3059
Bse21I	CCTNAGG	1 cut(s)	1908
Bse3DI	GCAATG	2 cut(s)	1183, 2055
Bse8I	GATNNNNATC	1 cut(s)	814
BseAI	TCCGGA	2 cut(s)	2567, 3705
BseBI	CCWGG	7 cut(s)	507, 663, 2133, 2266, 2544, 2711, 3419
BseGI	GGATG	8 cut(s)	523, 784, 790, 838, 904, 2564, 3161, 3311
BseJI	GATNNNNATC	1 cut(s)	814
BseLI	CCNNNNNNNGG	5 cut(s)	604, 1078, 1612, 2227, 2864
BseMI	GCAATG	2 cut(s)	1183, 2055
BseMII	CTCAG	9 cut(s)	867, 1748, 1827, 1922, 2291, 2355, 2390, 2438, 2768
BseNI	ACTGG	8 cut(s)	266, 595, 955, 1282, 1526, 1564, 1758, 3059
BseRI	GAGGAG	5 cut(s)	200, 2486, 2913, 3050, 3386
BseSI	GKGCMC	3 cut(s)	1728, 2551, 3653
BseXI	GCAGC	9 cut(s)	29, 1560, 2410, 2413, 2857, 2860, 2971, 3739, 3845
BseYI	CCCAGC	2 cut(s)	721, 2876
BsgI	GTGCAG	5 cut(s)	70, 410, 585, 3146, 3367
BshFI	GGCC	9 cut(s)	54, 63, 452, 597, 1297, 2157, 2603, 3279, 3342
BshNI	GGYRCC	4 cut(s)	342, 1495, 1723, 2546
BsiHKAI	GWGCWC	3 cut(s)	30, 707, 1641
BsiHKCI	CYCGRG	4 cut(s)	74, 1298, 1459, 1673
BslFI	GGGAC	2 cut(s)	1256, 2524
BslI	CCNNNNNNNGG	5 cut(s)	604, 1078, 1612, 2227, 2864
BsmAI	GTCTC	8 cut(s)	182, 918, 1316, 1456, 2033, 3196, 3722, 3788
BsmBI	CGTCTC	1 cut(s)	3722
BsmFI	GGGAC	2 cut(s)	1256, 2524
BsmI	GAATGC	1 cut(s)	2065
BsnI	GGCC	9 cut(s)	54, 63, 452, 597, 1297, 2157, 2603, 3279, 3342
BsoBI	CYCGRG	4 cut(s)	74, 1298, 1459, 1673
Bsp1286I	GDGCHC	7 cut(s)	30, 707, 1641, 1674, 1728, 2551, 3653
Bsp13I	TCCGGA	2 cut(s)	2567, 3705
Bsp1407I	TGTACA	3 cut(s)	280, 745, 3525
Bsp1720I	GCTNAGC	1 cut(s)	2424
Bsp19I	CCATGG	1 cut(s)	2863
BspACI	CCGC	8 cut(s)	60, 70, 137, 1616, 1619, 1622, 1625, 3434
BspANI	GGCC	9 cut(s)	54, 63, 452, 597, 1297, 2157, 2603, 3279, 3342
BspCNI	CTCAG	9 cut(s)	868, 1747, 1828, 1921, 2290, 2356, 2389, 2437, 2767
BspEI	TCCGGA	2 cut(s)	2567, 3705
BspHI	TCATGA	2 cut(s)	1018, 2583
BspMI	ACCTGC	1 cut(s)	382
BspOI	GCTAGC	1 cut(s)	3840
BspPI	GGATC	8 cut(s)	12, 364, 817, 1638, 1884, 1897, 2839, 3050
BspT107I	GGYRCC	4 cut(s)	342, 1495, 1723, 2546
BspTI	CTTAAG	1 cut(s)	3227
BsrDI	GCAATG	2 cut(s)	1183, 2055
BsrFI	RCCGGY	1 cut(s)	1612
BsrGI	TGTACA	3 cut(s)	280, 745, 3525
BsrI	ACTGG	8 cut(s)	266, 595, 955, 1282, 1526, 1564, 1758, 3059
BssAI	RCCGGY	1 cut(s)	1612
BssNAI	GTATAC	2 cut(s)	679, 1203
BssNI	GRCGYC	1 cut(s)	343
BssSI	CACGAG	1 cut(s)	1189
BssT1I	CCWWGG	4 cut(s)	21, 640, 2221, 2863
Bst1107I	GTATAC	2 cut(s)	679, 1203
Bst2BI	CACGAG	1 cut(s)	1189
Bst2UI	CCWGG	7 cut(s)	507, 663, 2133, 2266, 2544, 2711, 3419
Bst6I	CTCTTC	4 cut(s)	549, 2566, 2793, 3582
BstACI	GRCGYC	1 cut(s)	343
BstAFI	CTTAAG	1 cut(s)	3227
BstAPI	GCANNNNNTGC	2 cut(s)	1160, 2159
BstAUI	TGTACA	3 cut(s)	280, 745, 3525
BstBAI	YACGTR	3 cut(s)	1370, 1636, 3847
BstC8I	GCNNGC	6 cut(s)	141, 2155, 2164, 2421, 3352, 3838
BstDSI	CCRYGG	3 cut(s)	302, 1323, 2863
BstENI	CCTNNNNNAGG	1 cut(s)	2225
BstF5I	GGATG	8 cut(s)	523, 784, 790, 838, 904, 2564, 3161, 3311
BstH2I	RGCGCY	2 cut(s)	100, 346
BstHHI	GCGC	2 cut(s)	99, 345
BstMAI	GTCTC	8 cut(s)	182, 918, 1316, 1456, 2033, 3196, 3722, 3788
BstNI	CCWGG	7 cut(s)	507, 663, 2133, 2266, 2544, 2711, 3419
BstNSI	RCATGY	6 cut(s)	1810, 2246, 2347, 2488, 3088, 3354
BstPAI	GACNNNNGTC	1 cut(s)	642
BstSCI	CCNGG	9 cut(s)	36, 505, 661, 966, 2131, 2264, 2542, 2709, 3417
BstSFI	CTRYAG	1 cut(s)	2974
BstSLI	GKGCMC	3 cut(s)	1728, 2551, 3653
BstSNI	TACGTA	1 cut(s)	3847
BstV1I	GCAGC	9 cut(s)	29, 1560, 2410, 2413, 2857, 2860, 2971, 3739, 3845
BstX2I	RGATCY	4 cut(s)	4, 1889, 3055, 3067
BstXI	CCANNNNNNTGG	1 cut(s)	353
BstYI	RGATCY	4 cut(s)	4, 1889, 3055, 3067
BstZ17I	GTATAC	2 cut(s)	679, 1203
Bsu36I	CCTNAGG	1 cut(s)	1908
BsuI	GTATCC	1 cut(s)	1057
BsuRI	GGCC	9 cut(s)	54, 63, 452, 597, 1297, 2157, 2603, 3279, 3342
BtgI	CCRYGG	3 cut(s)	302, 1323, 2863
BtgZI	GCGATG	1 cut(s)	3165
BtrI	CACGTC	1 cut(s)	3732
BtsCI	GGATG	8 cut(s)	523, 784, 790, 838, 904, 2564, 3161, 3311
BtsI	GCAGTG	2 cut(s)	254, 2986
BtsIMutI	CAGTG	6 cut(s)	254, 1275, 2742, 2986, 3006, 3066
BveI	ACCTGC	1 cut(s)	382
Cac8I	GCNNGC	6 cut(s)	141, 2155, 2164, 2421, 3352, 3838
CaiI	CAGNNNCTG	2 cut(s)	1648, 2407
CciI	TCATGA	2 cut(s)	1018, 2583
CfoI	GCGC	2 cut(s)	99, 345
Cfr10I	RCCGGY	1 cut(s)	1612
Cfr13I	GGNCC	7 cut(s)	455, 596, 637, 644, 1296, 2602, 3708
DinI	GGCGCC	1 cut(s)	344
DraIII	CACNNNGTG	1 cut(s)	3390
DriI	GACNNNNNGTC	1 cut(s)	931
EaeI	YGGCCR	3 cut(s)	52, 61, 450
Eam1104I	CTCTTC	4 cut(s)	549, 2566, 2793, 3582
Eam1105I	GACNNNNNGTC	1 cut(s)	931
EarI	CTCTTC	4 cut(s)	549, 2566, 2793, 3582
EciI	GGCGGA	1 cut(s)	59
Ecl136II	GAGCTC	1 cut(s)	28
Eco105I	TACGTA	1 cut(s)	3847
Eco130I	CCWWGG	4 cut(s)	21, 640, 2221, 2863
Eco24I	GRGCYC	2 cut(s)	30, 1674
Eco32I	GATATC	1 cut(s)	2908
Eco47I	GGWCC	4 cut(s)	455, 637, 644, 3708
Eco47III	AGCGCT	1 cut(s)	98
Eco53kI	GAGCTC	1 cut(s)	28
Eco57I	CTGAAG	6 cut(s)	1637, 1904, 2351, 3092, 3314, 3711
Eco72I	CACGTG	1 cut(s)	1636
Eco81I	CCTNAGG	1 cut(s)	1908
Eco88I	CYCGRG	4 cut(s)	74, 1298, 1459, 1673
EcoICRI	GAGCTC	1 cut(s)	28
EcoNI	CCTNNNNNAGG	1 cut(s)	2225
EcoRI	GAATTC	2 cut(s)	3361, 3740
EcoRII	CCWGG	7 cut(s)	505, 661, 2131, 2264, 2542, 2709, 3417
EcoRV	GATATC	1 cut(s)	2908
EcoT14I	CCWWGG	4 cut(s)	21, 640, 2221, 2863
EcoT22I	ATGCAT	3 cut(s)	1165, 2310, 3090
EcoT38I	GRGCYC	2 cut(s)	30, 1674
EgeI	GGCGCC	1 cut(s)	344
EheI	GGCGCC	1 cut(s)	344
ErhI	CCWWGG	4 cut(s)	21, 640, 2221, 2863
Esp3I	CGTCTC	1 cut(s)	3722
FalI	AAGNNNNNCTT	2 cut(s)	393, 425
FaqI	GGGAC	2 cut(s)	1256, 2524
FauNDI	CATATG	1 cut(s)	2927
FbaI	TGATCA	2 cut(s)	685, 2301
FblI	GTMKAC	2 cut(s)	678, 1202
FokI	GGATG	8 cut(s)	530, 791, 797, 845, 911, 2551, 3168, 3318
FriOI	GRGCYC	2 cut(s)	30, 1674
GlaI	GCGC	2 cut(s)	98, 344
GsaI	CCCAGC	2 cut(s)	725, 2880
GsuI	CTGGAG	1 cut(s)	1625
HaeII	RGCGCY	2 cut(s)	100, 346
HaeIII	GGCC	9 cut(s)	54, 63, 452, 597, 1297, 2157, 2603, 3279, 3342
HhaI	GCGC	2 cut(s)	99, 345
Hin1I	GRCGYC	1 cut(s)	343
Hin6I	GCGC	2 cut(s)	97, 343
HinP1I	GCGC	2 cut(s)	97, 343
HindIII	AAGCTT	3 cut(s)	619, 2417, 3558
HphI	GGTGA	4 cut(s)	219, 968, 1292, 2893
Hpy166II	GTNNAC	7 cut(s)	283, 577, 679, 959, 989, 1203, 3674
Hpy8I	GTNNAC	7 cut(s)	283, 577, 679, 959, 989, 1203, 3674
Hpy99I	CGWCG	2 cut(s)	95, 298
HpyCH4IV	ACGT	8 cut(s)	991, 1369, 1635, 1686, 2785, 3731, 3817, 3846
HpySE526I	ACGT	8 cut(s)	991, 1369, 1635, 1686, 2785, 3731, 3817, 3846
Hsp92I	GRCGYC	1 cut(s)	343
HspAI	GCGC	2 cut(s)	97, 343
KasI	GGCGCC	1 cut(s)	342
Kpn2I	TCCGGA	2 cut(s)	2567, 3705
KpnI	GGTACC	1 cut(s)	1499
Ksp22I	TGATCA	2 cut(s)	685, 2301
LmnI	GCTCC	8 cut(s)	25, 104, 123, 289, 1213, 1461, 1644, 2900
Lsp1109I	GCAGC	9 cut(s)	29, 1560, 2410, 2413, 2857, 2860, 2971, 3739, 3845
LweI	GCATC	8 cut(s)	492, 1184, 2317, 2400, 3097, 3252, 3304, 3764
MaeII	ACGT	8 cut(s)	991, 1369, 1635, 1686, 2785, 3731, 3817, 3846
MaeIII	GTNAC	8 cut(s)	330, 1370, 1555, 1862, 2720, 2759, 3818, 3847
MfeI	CAATTG	2 cut(s)	1717, 1974
MflI	RGATCY	4 cut(s)	4, 1889, 3055, 3067
MhlI	GDGCHC	7 cut(s)	30, 707, 1641, 1674, 1728, 2551, 3653
Mly113I	GGCGCC	1 cut(s)	343
MlyI	GAGTC	4 cut(s)	173, 437, 920, 1115
MmeI	TCCRAC	7 cut(s)	126, 344, 876, 1246, 1917, 2233, 2994
Mph1103I	ATGCAT	3 cut(s)	1165, 2310, 3090
MroI	TCCGGA	2 cut(s)	2567, 3705
MroXI	GAANNNNTTC	2 cut(s)	3177, 3252
MseI	TTAA	9 cut(s)	624, 942, 1334, 1980, 2093, 3162, 3219, 3228, 3443
MslI	CAYNNNNRTG	3 cut(s)	2147, 2865, 2926
MspA1I	CMGCKG	3 cut(s)	60, 1136, 1799
MspCI	CTTAAG	1 cut(s)	3227
MspR9I	CCNGG	9 cut(s)	38, 507, 663, 968, 2133, 2266, 2544, 2711, 3419
MunI	CAATTG	2 cut(s)	1717, 1974
Mva1269I	GAATGC	1 cut(s)	2065
MvaI	CCWGG	7 cut(s)	507, 663, 2133, 2266, 2544, 2711, 3419
NarI	GGCGCC	1 cut(s)	343
NciI	CCSGG	2 cut(s)	38, 968
NcoI	CCATGG	1 cut(s)	2863
NdeI	CATATG	1 cut(s)	2927
NheI	GCTAGC	1 cut(s)	3836
NmeAIII	GCCGAG	1 cut(s)	228
NmuCI	GTSAC	3 cut(s)	1370, 1862, 3818
NsiI	ATGCAT	3 cut(s)	1165, 2310, 3090
NspI	RCATGY	6 cut(s)	1810, 2246, 2347, 2488, 3088, 3354
OliI	CACNNNNGTG	1 cut(s)	2865
PaeI	GCATGC	1 cut(s)	3354
PaeR7I	CTCGAG	2 cut(s)	74, 1673
PagI	TCATGA	2 cut(s)	1018, 2583
PcsI	WCGNNNNNNNCGW	2 cut(s)	99, 164
PctI	GAATGC	1 cut(s)	2065
PdmI	GAANNNNTTC	2 cut(s)	3177, 3252
PfeI	GAWTC	8 cut(s)	212, 521, 815, 1780, 1849, 2273, 2747, 3604
PflFI	GACNNNGTC	2 cut(s)	107, 2954
PflMI	CCANNNNNTGG	1 cut(s)	2864
PfoI	TCCNGGA	1 cut(s)	505
PleI	GAGTC	4 cut(s)	172, 436, 920, 1114
PluTI	GGCGCC	1 cut(s)	346
PmaCI	CACGTG	1 cut(s)	1636
PmlI	CACGTG	1 cut(s)	1636
PpsI	GAGTC	4 cut(s)	172, 436, 920, 1114
Ppu21I	YACGTR	3 cut(s)	1370, 1636, 3847
PshAI	GACNNNNGTC	1 cut(s)	642
PshBI	ATTAAT	1 cut(s)	3443
PsiI	TTATAA	1 cut(s)	1451
Psp124BI	GAGCTC	1 cut(s)	30
Psp6I	CCWGG	7 cut(s)	505, 661, 2131, 2264, 2542, 2709, 3417
PspCI	CACGTG	1 cut(s)	1636
PspFI	CCCAGC	2 cut(s)	721, 2876
PspGI	CCWGG	7 cut(s)	505, 661, 2131, 2264, 2542, 2709, 3417
PspPI	GGNCC	7 cut(s)	455, 596, 637, 644, 1296, 2602, 3708
PspXI	VCTCGAGB	2 cut(s)	74, 1673
PstNI	CAGNNNCTG	2 cut(s)	1648, 2407
PsuI	RGATCY	4 cut(s)	4, 1889, 3055, 3067
PsyI	GACNNNGTC	2 cut(s)	107, 2954
PvuII	CAGCTG	2 cut(s)	1136, 1799
RseI	CAYNNNNRTG	3 cut(s)	2147, 2865, 2926
SacI	GAGCTC	1 cut(s)	30
SaqAI	TTAA	9 cut(s)	624, 942, 1334, 1980, 2093, 3162, 3219, 3228, 3443
Sau96I	GGNCC	7 cut(s)	455, 596, 637, 644, 1296, 2602, 3708
SchI	GAGTC	4 cut(s)	173, 437, 920, 1115
ScrFI	CCNGG	9 cut(s)	38, 507, 663, 968, 2133, 2266, 2544, 2711, 3419
SduI	GDGCHC	7 cut(s)	30, 707, 1641, 1674, 1728, 2551, 3653
SfaNI	GCATC	8 cut(s)	492, 1184, 2317, 2400, 3097, 3252, 3304, 3764
SfcI	CTRYAG	1 cut(s)	2974
SfiI	GGCCNNNNNGGCC	1 cut(s)	60
SfoI	GGCGCC	1 cut(s)	344
Sfr274I	CTCGAG	2 cut(s)	74, 1673
SgrAI	CRCCGGYG	1 cut(s)	1612
SinI	GGWCC	4 cut(s)	455, 637, 644, 3708
SlaI	CTCGAG	2 cut(s)	74, 1673
SmiMI	CAYNNNNRTG	3 cut(s)	2147, 2865, 2926
SmlI	CTYRAG	8 cut(s)	74, 650, 1094, 1291, 1673, 2966, 3227, 3608
SmoI	CTYRAG	8 cut(s)	74, 650, 1094, 1291, 1673, 2966, 3227, 3608
SnaBI	TACGTA	1 cut(s)	3847
SphI	GCATGC	1 cut(s)	3354
SsiI	CCGC	8 cut(s)	60, 70, 137, 1616, 1619, 1622, 1625, 3434
SspDI	GGCGCC	1 cut(s)	342
SspI	AATATT	1 cut(s)	1332
SstI	GAGCTC	1 cut(s)	30
StyD4I	CCNGG	9 cut(s)	36, 505, 661, 966, 2131, 2264, 2542, 2709, 3417
StyI	CCWWGG	4 cut(s)	21, 640, 2221, 2863
TaiI	ACGT	8 cut(s)	994, 1372, 1638, 1689, 2788, 3734, 3820, 3849
TaqI	TCGA	9 cut(s)	75, 93, 167, 396, 1260, 1419, 1674, 2008, 2442
TatI	WGTACW	3 cut(s)	280, 745, 3525
TauI	GCSGC	5 cut(s)	63, 139, 1619, 1622, 1625
TfiI	GAWTC	8 cut(s)	212, 521, 815, 1780, 1849, 2273, 2747, 3604
Tru1I	TTAA	9 cut(s)	624, 942, 1334, 1980, 2093, 3162, 3219, 3228, 3443
Tru9I	TTAA	9 cut(s)	624, 942, 1334, 1980, 2093, 3162, 3219, 3228, 3443
TscAI	CASTG	6 cut(s)	254, 1282, 2749, 2986, 3006, 3066
TseFI	GTSAC	3 cut(s)	1370, 1862, 3818
TseI	GCWGC	9 cut(s)	17, 1573, 2398, 2401, 2870, 2873, 2984, 3752, 3833
Tsp45I	GTSAC	3 cut(s)	1370, 1862, 3818
TspGWI	ACGGA	3 cut(s)	1340, 1541, 1546
TspRI	CASTG	6 cut(s)	254, 1282, 2749, 2986, 3006, 3066
Tth111I	GACNNNGTC	2 cut(s)	107, 2954
Van91I	CCANNNNNTGG	1 cut(s)	2864
Vha464I	CTTAAG	1 cut(s)	3227
VpaK11BI	GGWCC	4 cut(s)	455, 637, 644, 3708
VspI	ATTAAT	1 cut(s)	3443
XagI	CCTNNNNNAGG	1 cut(s)	2225
XapI	RAATTY	8 cut(s)	1012, 1256, 1740, 1900, 2099, 2114, 3361, 3740
XbaI	TCTAGA	1 cut(s)	532
XceI	RCATGY	6 cut(s)	1810, 2246, 2347, 2488, 3088, 3354
XcmI	CCANNNNNNNNNTGG	2 cut(s)	587, 1943
XhoI	CTCGAG	2 cut(s)	74, 1673
XmaJI	CCTAGG	1 cut(s)	2221
XmiI	GTMKAC	2 cut(s)	678, 1202
XmnI	GAANNNNTTC	2 cut(s)	3177, 3252
Zsp2I	ATGCAT	3 cut(s)	1165, 2310, 3090

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.