Rmu_sc0000449.1_g000056

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000449.1
OG0006676
Physical Location & Seq
Reverse (-)
245138 .. 246381
1244 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1146 bp
atgcaaagaacaggtgttcgaccagatagcgttgttgcatctttaatgcattttgctcaaacatccttaaagggtattggaaaatgtcaagtttggaaaaagccggatagcttaccagaacatgaccaaaggactattgtggaaacccttataagcttaatgccaacagaaaaaagctgttcggtgccactgagttttatgtttggaatgctgaggatgtcaatcatggtggatgctgcaattgcctgcaggctcgagattgaaaggaggattgcttacaggttggagatggttacactagatgatatccttataccgtccatttttgcagggggttcactgtatgatgttgataccgttcatcgaatactggtgaacttcctacagcggattgaagaagaagaagatgaagactgtggatatgagtcggaaggcattgctaattctccgagccatggttcattgttgaaagttggacggcttattgactcatatctagctgaaattgctcctgacccatacctcagcttgcaaaagttcgttgctatgattgaagtactgcctgattatgctcgagtcatcgatgatggactttacagggctgttgatatatatttgaaggtacattcaatgctaactgaacaagaatgcaagaagctctgcaagtttatagactgtcaaaagctctctcaagaagcctgcaaccatgctgcacaaaatgatcggctcccagtacaaatgacagtgagggttctgtatttcgagcaggtccgattgaagactgcactgtcaggaaactcaggagatgggtttgtctcgcagagaataagcagcggggtaccaagtgcagccatgtccccccgagataactatgcttcattgaggagagaaaaccgagaactgaagctggagatctcaagaatgagagtgaggctgagcgagttggagaaagagcagttgtttatgaaacaaggaatgatggacaagacaggaaatggcaaaactttcttgacctcaatctctaaggggattggaaaaatgggaatttttagtggtcaagcaggaggtaaacgacagaaatcgagtaggaagtcccggggatcggaggggaaaactggtaggggtaggaggcactctctttcctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

381

Amino Acids

42.72

Weight (kDa)

8.9

Isoelectric Point (pI)

46.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 1 - 234 1.9e-77 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 152
AasI GACNNNNNNGTC 1 cut(s) 787
Acc36I ACCTGC 1 cut(s) 757
Acc65I GGTACC 1 cut(s) 838
AccB1I GGYRCC 2 cut(s) 184, 838
AciI CCGC 2 cut(s) 388, 834
AclWI GGATC 1 cut(s) 1108
AcsI RAATTY 1 cut(s) 1044
AcuI CTGAAG 1 cut(s) 923
AfaI GTAC 4 cut(s) 558, 624, 735, 840
AfiI CCNNNNNNNGG 2 cut(s) 455, 1102
AgsI TTSAA 7 cut(s) 263, 395, 469, 554, 619, 630, 778
AluBI AGCT 8 cut(s) 111, 156, 177, 500, 528, 658, 685, 907
AluI AGCT 8 cut(s) 111, 156, 177, 500, 528, 658, 685, 907
Alw26I GTCTC 1 cut(s) 820
AlwI GGATC 1 cut(s) 1108
Ama87I CYCGRG 4 cut(s) 254, 573, 861, 1095
ApeKI GCWGC 4 cut(s) 236, 710, 831, 848
ApoI RAATTY 1 cut(s) 1044
Asp718I GGTACC 1 cut(s) 838
AspS9I GGNCC 1 cut(s) 769
AsuC2I CCSGG 2 cut(s) 1096, 1097
AsuHPI GGTGA 1 cut(s) 385
AvaI CYCGRG 4 cut(s) 254, 573, 861, 1095
AvaII GGWCC 1 cut(s) 769
BanI GGYRCC 2 cut(s) 184, 838
BbsI GAAGAC 2 cut(s) 417, 785
BbvCI CCTCAGC 2 cut(s) 212, 524
BbvI GCAGC 4 cut(s) 223, 697, 843, 860
BccI CCATC 4 cut(s) 283, 581, 800, 973
BceAI ACGGC 1 cut(s) 494
BcnI CCSGG 2 cut(s) 1096, 1097
BcoDI GTCTC 1 cut(s) 820
BfaI CTAG 3 cut(s) 299, 497, 1144
BfmI CTRYAG 2 cut(s) 247, 383
BfuAI ACCTGC 1 cut(s) 757
BglII AGATCT 1 cut(s) 912
BisI GCNGC 4 cut(s) 237, 711, 832, 849
BlpI GCTNAGC 1 cut(s) 935
BlsI GCNGC 4 cut(s) 238, 712, 833, 850
BmcAI AGTACT 1 cut(s) 558
Bme1390I CCNGG 2 cut(s) 1096, 1097
Bme18I GGWCC 1 cut(s) 769
BmeT110I CYCGRG 4 cut(s) 254, 573, 861, 1095
BmgT120I GGNCC 1 cut(s) 769
BmiI GGNNCC 3 cut(s) 186, 728, 840
BmrFI CCNGG 2 cut(s) 1096, 1097
BmrI ACTGGG 1 cut(s) 725
BmsI GCATC 2 cut(s) 47, 223
BmuI ACTGGG 1 cut(s) 725
BpiI GAAGAC 2 cut(s) 417, 785
BplI GAGNNNNNCTC 1 cut(s) 1120
BpmI CTGGAG 1 cut(s) 929
Bpu10I CCTNAGC 2 cut(s) 212, 524
Bpu1102I GCTNAGC 1 cut(s) 935
BpuEI CTTGAG 2 cut(s) 675, 901
BpuMI CCSGG 2 cut(s) 1096, 1097
Bsa29I ATCGAT 1 cut(s) 582
BsaBI GATNNNNATC 1 cut(s) 221
BsaJI CCNNGG 3 cut(s) 454, 1095, 1096
Bsc4I CCNNNNNNNGG 2 cut(s) 455, 1102
Bse1I ACTGG 3 cut(s) 375, 731, 1120
Bse3DI GCAATG 1 cut(s) 435
Bse8I GATNNNNATC 1 cut(s) 221
BseCI ATCGAT 1 cut(s) 582
BseDI CCNNGG 3 cut(s) 454, 1095, 1096
BseGI GGATG 3 cut(s) 62, 222, 238
BseJI GATNNNNATC 1 cut(s) 221
BseLI CCNNNNNNNGG 2 cut(s) 455, 1102
BseMI GCAATG 1 cut(s) 435
BseMII CTCAG 5 cut(s) 182, 203, 538, 813, 926
BseNI ACTGG 3 cut(s) 375, 731, 1120
BseRI GAGGAG 1 cut(s) 898
BseXI GCAGC 4 cut(s) 223, 697, 843, 860
BsgI GTGCAG 3 cut(s) 696, 768, 867
BshNI GGYRCC 2 cut(s) 184, 838
BshVI ATCGAT 1 cut(s) 582
BsiHKCI CYCGRG 4 cut(s) 254, 573, 861, 1095
BsiSI CCGG 2 cut(s) 104, 1096
BslFI GGGAC 2 cut(s) 841, 1078
BslI CCNNNNNNNGG 2 cut(s) 455, 1102
BsmAI GTCTC 1 cut(s) 820
BsmFI GGGAC 2 cut(s) 841, 1078
BsmI GAATGC 2 cut(s) 213, 653
BsoBI CYCGRG 4 cut(s) 254, 573, 861, 1095
Bsp143I GATC 3 cut(s) 721, 912, 1100
Bsp1720I GCTNAGC 1 cut(s) 935
Bsp19I CCATGG 1 cut(s) 454
BspACI CCGC 2 cut(s) 388, 834
BspCNI CTCAG 5 cut(s) 183, 204, 537, 812, 927
BspDI ATCGAT 1 cut(s) 582
BspLI GGNNCC 3 cut(s) 186, 728, 840
BspMAI CTGCAG 1 cut(s) 251
BspMI ACCTGC 1 cut(s) 757
BspPI GGATC 1 cut(s) 1108
BspT107I GGYRCC 2 cut(s) 184, 838
BsrDI GCAATG 1 cut(s) 435
BsrI ACTGG 3 cut(s) 375, 731, 1120
BssECI CCNNGG 3 cut(s) 454, 1095, 1096
BssMI GATC 3 cut(s) 721, 912, 1100
BssT1I CCWWGG 1 cut(s) 454
Bst4CI ACNGT 7 cut(s) 318, 342, 358, 416, 677, 745, 789
BstC8I GCNNGC 4 cut(s) 247, 251, 530, 700
BstDEI CTNAG 6 cut(s) 191, 212, 524, 799, 935, 1023
BstDSI CCRYGG 1 cut(s) 454
BstF5I GGATG 3 cut(s) 62, 222, 238
BstKTI GATC 3 cut(s) 724, 915, 1103
BstMAI GTCTC 1 cut(s) 820
BstMBI GATC 3 cut(s) 721, 912, 1100
BstMWI GCNNNNNNNGC 2 cut(s) 242, 506
BstSCI CCNGG 2 cut(s) 1094, 1095
BstSFI CTRYAG 2 cut(s) 247, 383
BstV1I GCAGC 4 cut(s) 223, 697, 843, 860
BstV2I GAAGAC 2 cut(s) 417, 785
BstX2I RGATCY 1 cut(s) 912
BstYI RGATCY 1 cut(s) 912
Bsu15I ATCGAT 1 cut(s) 582
BsuTUI ATCGAT 1 cut(s) 582
BtgI CCRYGG 1 cut(s) 454
BtsCI GGATG 3 cut(s) 62, 222, 238
BtsIMutI CAGTG 4 cut(s) 188, 338, 750, 785
BveI ACCTGC 1 cut(s) 757
Cac8I GCNNGC 4 cut(s) 247, 251, 530, 700
Cfr13I GGNCC 1 cut(s) 769
Cfr9I CCCGGG 1 cut(s) 1095
ClaI ATCGAT 1 cut(s) 582
Csp6I GTAC 4 cut(s) 557, 623, 734, 839
CspCI CAANNNNNGTGG 2 cut(s) 210, 245
CviAII CATG 5 cut(s) 122, 226, 455, 707, 853
CviQI GTAC 4 cut(s) 557, 623, 734, 839
DdeI CTNAG 6 cut(s) 191, 212, 524, 799, 935, 1023
DpnI GATC 3 cut(s) 723, 914, 1102
DpnII GATC 3 cut(s) 721, 912, 1100
DrdI GACNNNNNNGTC 1 cut(s) 787
DseDI GACNNNNNNGTC 1 cut(s) 787
Eco130I CCWWGG 1 cut(s) 454
Eco32I GATATC 1 cut(s) 307
Eco47I GGWCC 1 cut(s) 769
Eco57I CTGAAG 1 cut(s) 923
Eco88I CYCGRG 4 cut(s) 254, 573, 861, 1095
EcoRV GATATC 1 cut(s) 307
EcoT14I CCWWGG 1 cut(s) 454
EcoT22I ATGCAT 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 454
FaeI CATG 5 cut(s) 125, 229, 458, 710, 856
FaqI GGGAC 2 cut(s) 841, 1078
FatI CATG 5 cut(s) 121, 225, 454, 706, 852
FauI CCCGC 1 cut(s) 827
Fnu4HI GCNGC 4 cut(s) 237, 711, 832, 849
FokI GGATG 3 cut(s) 49, 229, 245
Fsp4HI GCNGC 4 cut(s) 237, 711, 832, 849
FspBI CTAG 3 cut(s) 299, 497, 1144
GluI GCNGC 4 cut(s) 237, 711, 832, 849
GsuI CTGGAG 1 cut(s) 929
HapII CCGG 2 cut(s) 104, 1096
Hin1II CATG 5 cut(s) 125, 229, 458, 710, 856
HindIII AAGCTT 1 cut(s) 154
HinfI GANTC 3 cut(s) 425, 488, 576
HpaII CCGG 2 cut(s) 104, 1096
HphI GGTGA 1 cut(s) 385
Hpy166II GTNNAC 3 cut(s) 338, 376, 1070
Hpy188I TCNGA 4 cut(s) 430, 450, 773, 1105
Hpy188III TCNNGA 7 cut(s) 256, 512, 692, 792, 801, 918, 1009
Hpy8I GTNNAC 3 cut(s) 338, 376, 1070
HpyAV CCTTC 2 cut(s) 425, 613
HpyCH4III ACNGT 7 cut(s) 318, 342, 358, 416, 677, 745, 789
HpyF10VI GCNNNNNNNGC 2 cut(s) 242, 506
HpyF3I CTNAG 6 cut(s) 191, 212, 524, 799, 935, 1023
Hsp92II CATG 5 cut(s) 125, 229, 458, 710, 856
KpnI GGTACC 1 cut(s) 842
Kzo9I GATC 3 cut(s) 721, 912, 1100
LmnI GCTCC 2 cut(s) 514, 732
Lsp1109I GCAGC 4 cut(s) 223, 697, 843, 860
LweI GCATC 2 cut(s) 47, 223
MaeI CTAG 3 cut(s) 299, 497, 1144
MaeIII GTNAC 1 cut(s) 292
MalI GATC 3 cut(s) 723, 914, 1102
MboI GATC 3 cut(s) 721, 912, 1100
MboII GAAGA 6 cut(s) 407, 410, 413, 416, 422, 790
MfeI CAATTG 1 cut(s) 240
MflI RGATCY 1 cut(s) 912
MluCI AATT 4 cut(s) 240, 442, 504, 1044
MlyI GAGTC 3 cut(s) 434, 482, 585
MmeI TCCRAC 4 cut(s) 264, 408, 454, 924
Mph1103I ATGCAT 1 cut(s) 51
MseI TTAA 3 cut(s) 44, 68, 158
MspA1I CMGCKG 2 cut(s) 388, 834
MspI CCGG 2 cut(s) 104, 1096
MspR9I CCNGG 2 cut(s) 1096, 1097
MunI CAATTG 1 cut(s) 240
Mva1269I GAATGC 2 cut(s) 213, 653
MwoI GCNNNNNNNGC 2 cut(s) 242, 506
NciI CCSGG 2 cut(s) 1096, 1097
NcoI CCATGG 1 cut(s) 454
NdeII GATC 3 cut(s) 721, 912, 1100
NlaIII CATG 5 cut(s) 125, 229, 458, 710, 856
NlaIV GGNNCC 3 cut(s) 186, 728, 840
NsiI ATGCAT 1 cut(s) 51
PaeR7I CTCGAG 2 cut(s) 254, 573
PctI GAATGC 2 cut(s) 213, 653
PkrI GCNGC 4 cut(s) 238, 712, 833, 850
PleI GAGTC 3 cut(s) 433, 482, 584
PpsI GAGTC 3 cut(s) 433, 482, 584
PsiI TTATAA 1 cut(s) 152
PspN4I GGNNCC 3 cut(s) 186, 728, 840
PspPI GGNCC 1 cut(s) 769
PspXI VCTCGAGB 1 cut(s) 573
PstI CTGCAG 1 cut(s) 251
PsuI RGATCY 1 cut(s) 912
RsaI GTAC 4 cut(s) 558, 624, 735, 840
RsaNI GTAC 4 cut(s) 557, 623, 734, 839
SaqAI TTAA 3 cut(s) 44, 68, 158
SatI GCNGC 4 cut(s) 237, 711, 832, 849
Sau3AI GATC 3 cut(s) 721, 912, 1100
Sau96I GGNCC 1 cut(s) 769
SbfI CCTGCAGG 1 cut(s) 251
ScaI AGTACT 1 cut(s) 558
SchI GAGTC 3 cut(s) 434, 482, 585
ScrFI CCNGG 2 cut(s) 1096, 1097
SdaI CCTGCAGG 1 cut(s) 251
SfaNI GCATC 2 cut(s) 47, 223
SfcI CTRYAG 2 cut(s) 247, 383
Sfr274I CTCGAG 2 cut(s) 254, 573
SinI GGWCC 1 cut(s) 769
SlaI CTCGAG 2 cut(s) 254, 573
SmaI CCCGGG 1 cut(s) 1097
SmlI CTYRAG 4 cut(s) 254, 573, 690, 916
SmoI CTYRAG 4 cut(s) 254, 573, 690, 916
Sse8387I CCTGCAGG 1 cut(s) 251
Sse9I AATT 4 cut(s) 240, 442, 504, 1044
SsiI CCGC 2 cut(s) 388, 834
SspMI CTAG 3 cut(s) 299, 497, 1144
StyD4I CCNGG 2 cut(s) 1094, 1095
StyI CCWWGG 1 cut(s) 454
TaaI ACNGT 7 cut(s) 318, 342, 358, 416, 677, 745, 789
TaqI TCGA 7 cut(s) 19, 255, 364, 574, 582, 762, 1082
TasI AATT 4 cut(s) 240, 442, 504, 1044
TatI WGTACW 2 cut(s) 556, 733
Tru1I TTAA 3 cut(s) 44, 68, 158
Tru9I TTAA 3 cut(s) 44, 68, 158
TscAI CASTG 4 cut(s) 195, 345, 750, 792
TseI GCWGC 4 cut(s) 236, 710, 831, 848
TspDTI ATGAA 5 cut(s) 350, 423, 450, 867, 980
TspMI CCCGGG 1 cut(s) 1095
TspRI CASTG 4 cut(s) 195, 345, 750, 792
VpaK11BI GGWCC 1 cut(s) 769
XapI RAATTY 1 cut(s) 1044
XhoI CTCGAG 2 cut(s) 254, 573
XmaI CCCGGG 1 cut(s) 1095
XspI CTAG 3 cut(s) 299, 497, 1144
ZrmI AGTACT 1 cut(s) 558
Zsp2I ATGCAT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.