Rmu_sc0000882.1_g000040

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000882.1
OG0016946
Physical Location & Seq
Reverse (-)
214291 .. 217336
3046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000882.1_g000040.1.cds

Sequence Viewer

Length: 822 bp
atggtggtgctctctcctccagggattatgagtgaggccatggttttgagctgctctggtttctttatctccacgcccttcactcctagccttctcagcactgcaacacagaacggagatgaggtagctccgatcgaagcttcgtcgtcgtcttcaggcgtggctgcgcctttttgtggttttgtaatctttgatcgattggaatctcaccagatcggaggcggtggtcgtcagaattggattgggggacaaagagtgtcgtgccggtggtcgcctgaatcggatggaggagagagagagtcgtgctggtggtcgccggaatcggaggcggagaccaaagacgtgaaaaatctagggtcgaggagagagagattcgtgctggtggtcgccggaatcggaggcggagaccaaagacatagagaccagaaacgaaatcttgaagctgctatcacgctcgcgttgattgtaatcattgcatctgataattggattagtgaggacggagaaaacctaggccacggtggtcaccacctcacaactcctgcagtcttgaacccaactgatttctatcccatttttgtatttgttccatcaaaggtggacctccccagatttaagaatgaagaagtgaaggttgagctagaggaagggaatgtccttcagatcaatggagagagaagcagtgagccagaggagaagaacgacaagtggcacagcgtagagaggagcagtggcaagttcttgaggaggttcaggctgccagtacgtcgtcgcctccacctcaccagagcctcgagttcatcgtcaagtcactgtaagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0016043 GO:0022607 GO:0043933 GO:0044085 GO:0050896 GO:0051259 GO:0065003 GO:0071840
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

273

Amino Acids

30.08

Weight (kDa)

6.09

Isoelectric Point (pI)

69.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 458
AciI CCGC 3 cut(s) 222, 329, 402
AcuI CTGAAG 2 cut(s) 138, 644
AfaI GTAC 1 cut(s) 765
AfiI CCNNNNNNNGG 1 cut(s) 176
AgsI TTSAA 2 cut(s) 440, 553
AjiI CACGTC 1 cut(s) 343
AjnI CCWGG 1 cut(s) 19
AluBI AGCT 5 cut(s) 51, 128, 140, 443, 640
AluI AGCT 5 cut(s) 51, 128, 140, 443, 640
Alw21I GWGCWC 1 cut(s) 12
Alw26I GTCTC 3 cut(s) 326, 399, 414
Ama87I CYCGRG 1 cut(s) 793
AoxI GGCC 2 cut(s) 36, 514
ApeKI GCWGC 4 cut(s) 51, 164, 443, 757
AspA2I CCTAGG 1 cut(s) 511
AspLEI GCGC 1 cut(s) 169
AspS9I GGNCC 1 cut(s) 601
AsuHPI GGTGA 3 cut(s) 200, 518, 775
AvaI CYCGRG 1 cut(s) 793
AvaII GGWCC 1 cut(s) 601
AvrII CCTAGG 1 cut(s) 511
BbsI GAAGAC 1 cut(s) 144
Bbv12I GWGCWC 1 cut(s) 12
BbvI GCAGC 4 cut(s) 38, 151, 430, 744
BccI CCATC 2 cut(s) 278, 598
BciT130I CCWGG 1 cut(s) 21
BcoDI GTCTC 3 cut(s) 326, 399, 414
BfaI CTAG 4 cut(s) 87, 353, 512, 641
BfmI CTRYAG 1 cut(s) 543
BisI GCNGC 4 cut(s) 52, 165, 444, 758
BlnI CCTAGG 1 cut(s) 511
BlsI GCNGC 4 cut(s) 53, 166, 445, 759
Bme1390I CCNGG 1 cut(s) 21
Bme18I GGWCC 1 cut(s) 601
BmeT110I CYCGRG 1 cut(s) 793
BmgBI CACGTC 1 cut(s) 343
BmgT120I GGNCC 1 cut(s) 601
BmrFI CCNGG 1 cut(s) 21
BmsI GCATC 1 cut(s) 485
BpiI GAAGAC 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 763
Bsa29I ATCGAT 1 cut(s) 196
BsaBI GATNNNNATC 3 cut(s) 202, 467, 567
BsaI GGTCTC 3 cut(s) 326, 399, 414
BsaJI CCNNGG 4 cut(s) 20, 39, 511, 517
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 1 cut(s) 176
Bse118I RCCGGY 1 cut(s) 264
Bse1I ACTGG 1 cut(s) 761
Bse3DI GCAATG 1 cut(s) 471
Bse8I GATNNNNATC 3 cut(s) 202, 467, 567
BseBI CCWGG 1 cut(s) 21
BseCI ATCGAT 1 cut(s) 196
BseDI CCNNGG 4 cut(s) 20, 39, 511, 517
BseGI GGATG 1 cut(s) 289
BseJI GATNNNNATC 3 cut(s) 202, 467, 567
BseLI CCNNNNNNNGG 1 cut(s) 176
BseMI GCAATG 1 cut(s) 471
BseMII CTCAG 1 cut(s) 109
BseNI ACTGG 1 cut(s) 761
BseRI GAGGAG 6 cut(s) 6, 303, 376, 707, 739, 760
BseXI GCAGC 4 cut(s) 38, 151, 430, 744
Bsh1236I CGCG 1 cut(s) 458
Bsh1285I CGRYCG 1 cut(s) 135
BshFI GGCC 2 cut(s) 38, 516
BshVI ATCGAT 1 cut(s) 196
BsiEI CGRYCG 1 cut(s) 135
BsiHKAI GWGCWC 1 cut(s) 12
BsiHKCI CYCGRG 1 cut(s) 793
BsiSI CCGG 3 cut(s) 265, 317, 390
BslFI GGGAC 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 176
BsmAI GTCTC 3 cut(s) 326, 399, 414
BsmFI GGGAC 1 cut(s) 261
BsnI GGCC 2 cut(s) 38, 516
Bso31I GGTCTC 3 cut(s) 326, 399, 414
BsoBI CYCGRG 1 cut(s) 793
Bsp1286I GDGCHC 1 cut(s) 12
Bsp143I GATC 4 cut(s) 132, 193, 213, 663
Bsp19I CCATGG 1 cut(s) 39
BspACI CCGC 3 cut(s) 222, 329, 402
BspANI GGCC 2 cut(s) 38, 516
BspCNI CTCAG 1 cut(s) 108
BspDI ATCGAT 1 cut(s) 196
BspFNI CGCG 1 cut(s) 458
BspMAI CTGCAG 1 cut(s) 547
BspTNI GGTCTC 3 cut(s) 326, 399, 414
BsrDI GCAATG 1 cut(s) 471
BsrFI RCCGGY 1 cut(s) 264
BsrI ACTGG 1 cut(s) 761
BssAI RCCGGY 1 cut(s) 264
BssECI CCNNGG 4 cut(s) 20, 39, 511, 517
BssMI GATC 4 cut(s) 132, 193, 213, 663
BssT1I CCWWGG 2 cut(s) 39, 511
Bst2UI CCWGG 1 cut(s) 21
Bst4CI ACNGT 2 cut(s) 521, 815
BstC8I GCNNGC 1 cut(s) 456
BstDEI CTNAG 1 cut(s) 95
BstDSI CCRYGG 2 cut(s) 39, 517
BstEII GGTNACC 1 cut(s) 524
BstF5I GGATG 1 cut(s) 289
BstFNI CGCG 1 cut(s) 458
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 4 cut(s) 135, 196, 216, 666
BstMAI GTCTC 3 cut(s) 326, 399, 414
BstMBI GATC 4 cut(s) 132, 193, 213, 663
BstMCI CGRYCG 1 cut(s) 135
BstMWI GCNNNNNNNGC 1 cut(s) 96
BstNI CCWGG 1 cut(s) 21
BstPI GGTNACC 1 cut(s) 524
BstSCI CCNGG 1 cut(s) 19
BstSFI CTRYAG 1 cut(s) 543
BstUI CGCG 1 cut(s) 458
BstV1I GCAGC 4 cut(s) 38, 151, 430, 744
BstV2I GAAGAC 1 cut(s) 144
Bsu15I ATCGAT 1 cut(s) 196
BsuRI GGCC 2 cut(s) 38, 516
BsuTUI ATCGAT 1 cut(s) 196
BtgI CCRYGG 2 cut(s) 39, 517
BtrI CACGTC 1 cut(s) 343
BtsCI GGATG 1 cut(s) 289
BtsI GCAGTG 3 cut(s) 99, 688, 736
BtsIMutI CAGTG 4 cut(s) 99, 688, 736, 811
Cac8I GCNNGC 1 cut(s) 456
CfoI GCGC 1 cut(s) 169
Cfr10I RCCGGY 1 cut(s) 264
Cfr13I GGNCC 1 cut(s) 601
ClaI ATCGAT 1 cut(s) 196
Csp6I GTAC 1 cut(s) 764
CviAII CATG 1 cut(s) 40
CviQI GTAC 1 cut(s) 764
DdeI CTNAG 1 cut(s) 95
DpnI GATC 4 cut(s) 134, 195, 215, 665
DpnII GATC 4 cut(s) 132, 193, 213, 663
EciI GGCGGA 2 cut(s) 344, 417
Eco130I CCWWGG 2 cut(s) 39, 511
Eco31I GGTCTC 3 cut(s) 326, 399, 414
Eco47I GGWCC 1 cut(s) 601
Eco57I CTGAAG 2 cut(s) 138, 644
Eco88I CYCGRG 1 cut(s) 793
Eco91I GGTNACC 1 cut(s) 524
EcoO65I GGTNACC 1 cut(s) 524
EcoRII CCWGG 1 cut(s) 19
EcoT14I CCWWGG 2 cut(s) 39, 511
ErhI CCWWGG 2 cut(s) 39, 511
FaeI CATG 1 cut(s) 43
FaiI YATR 3 cut(s) 29, 41, 417
FaqI GGGAC 1 cut(s) 261
FatI CATG 1 cut(s) 39
Fnu4HI GCNGC 4 cut(s) 52, 165, 444, 758
FokI GGATG 1 cut(s) 296
Fsp4HI GCNGC 4 cut(s) 52, 165, 444, 758
FspBI CTAG 4 cut(s) 87, 353, 512, 641
GlaI GCGC 1 cut(s) 168
GluI GCNGC 4 cut(s) 52, 165, 444, 758
HaeIII GGCC 2 cut(s) 38, 516
HapII CCGG 3 cut(s) 265, 317, 390
HhaI GCGC 1 cut(s) 169
Hin1II CATG 1 cut(s) 43
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HindIII AAGCTT 1 cut(s) 138
HinfI GANTC 6 cut(s) 203, 278, 299, 320, 372, 393
HpaII CCGG 3 cut(s) 265, 317, 390
HphI GGTGA 3 cut(s) 200, 518, 775
Hpy166II GTNNAC 1 cut(s) 601
Hpy188I TCNGA 8 cut(s) 132, 218, 234, 283, 325, 398, 481, 663
Hpy188III TCNNGA 3 cut(s) 437, 550, 742
Hpy8I GTNNAC 1 cut(s) 601
Hpy99I CGWCG 4 cut(s) 148, 151, 771, 774
HpyAV CCTTC 5 cut(s) 88, 101, 625, 641, 668
HpyCH4III ACNGT 2 cut(s) 521, 815
HpyCH4IV ACGT 2 cut(s) 342, 766
HpyCH4V TGCA 3 cut(s) 104, 476, 545
HpyF10VI GCNNNNNNNGC 1 cut(s) 96
HpyF3I CTNAG 1 cut(s) 95
HpySE526I ACGT 2 cut(s) 342, 766
Hsp92II CATG 1 cut(s) 43
HspAI GCGC 1 cut(s) 167
Kzo9I GATC 4 cut(s) 132, 193, 213, 663
LmnI GCTCC 2 cut(s) 133, 726
Lsp1109I GCAGC 4 cut(s) 38, 151, 430, 744
LweI GCATC 1 cut(s) 485
MaeI CTAG 4 cut(s) 87, 353, 512, 641
MaeII ACGT 2 cut(s) 342, 766
MaeIII GTNAC 2 cut(s) 524, 809
MalI GATC 4 cut(s) 134, 195, 215, 665
MboI GATC 4 cut(s) 132, 193, 213, 663
MboII GAAGA 3 cut(s) 144, 635, 709
MhlI GDGCHC 1 cut(s) 12
MluCI AATT 2 cut(s) 235, 484
MlyI GAGTC 1 cut(s) 308
MseI TTAA 1 cut(s) 615
MspI CCGG 3 cut(s) 265, 317, 390
MspR9I CCNGG 1 cut(s) 21
MvaI CCWGG 1 cut(s) 21
MvnI CGCG 1 cut(s) 458
MwoI GCNNNNNNNGC 1 cut(s) 96
NcoI CCATGG 1 cut(s) 39
NdeII GATC 4 cut(s) 132, 193, 213, 663
NlaIII CATG 1 cut(s) 43
NmuCI GTSAC 2 cut(s) 524, 809
PaeR7I CTCGAG 1 cut(s) 793
PcsI WCGNNNNNNNCGW 1 cut(s) 800
PfeI GAWTC 5 cut(s) 203, 278, 320, 372, 393
PkrI GCNGC 4 cut(s) 53, 166, 445, 759
Ple19I CGATCG 1 cut(s) 135
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
Psp6I CCWGG 1 cut(s) 19
PspEI GGTNACC 1 cut(s) 524
PspGI CCWGG 1 cut(s) 19
PspPI GGNCC 1 cut(s) 601
PspXI VCTCGAGB 1 cut(s) 793
PstI CTGCAG 1 cut(s) 547
PvuI CGATCG 1 cut(s) 135
RsaI GTAC 1 cut(s) 765
RsaNI GTAC 1 cut(s) 764
SaqAI TTAA 1 cut(s) 615
SatI GCNGC 4 cut(s) 52, 165, 444, 758
Sau3AI GATC 4 cut(s) 132, 193, 213, 663
Sau96I GGNCC 1 cut(s) 601
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 1 cut(s) 21
SduI GDGCHC 1 cut(s) 12
SfaNI GCATC 1 cut(s) 485
SfcI CTRYAG 1 cut(s) 543
Sfr274I CTCGAG 1 cut(s) 793
SinI GGWCC 1 cut(s) 601
SlaI CTCGAG 1 cut(s) 793
SmlI CTYRAG 2 cut(s) 742, 793
SmoI CTYRAG 2 cut(s) 742, 793
Sse9I AATT 2 cut(s) 235, 484
SsiI CCGC 3 cut(s) 222, 329, 402
SspMI CTAG 4 cut(s) 87, 353, 512, 641
StyD4I CCNGG 1 cut(s) 19
StyI CCWWGG 2 cut(s) 39, 511
TaaI ACNGT 2 cut(s) 521, 815
TaiI ACGT 2 cut(s) 345, 769
TaqI TCGA 4 cut(s) 135, 196, 359, 794
TasI AATT 2 cut(s) 235, 484
TfiI GAWTC 5 cut(s) 203, 278, 320, 372, 393
Tru1I TTAA 1 cut(s) 615
Tru9I TTAA 1 cut(s) 615
TscAI CASTG 4 cut(s) 106, 688, 736, 818
TseFI GTSAC 2 cut(s) 524, 809
TseI GCWGC 4 cut(s) 51, 164, 443, 757
Tsp45I GTSAC 2 cut(s) 524, 809
TspDTI ATGAA 2 cut(s) 636, 789
TspGWI ACGGA 2 cut(s) 129, 516
TspRI CASTG 4 cut(s) 106, 688, 736, 818
VpaK11BI GGWCC 1 cut(s) 601
XhoI CTCGAG 1 cut(s) 793
XmaJI CCTAGG 1 cut(s) 511
XspI CTAG 4 cut(s) 87, 353, 512, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.