Rh5BG113800

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0016946
Physical Location & Seq
Reverse (-)
10653949 .. 10658628
4680 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG113800.1

Sequence Viewer

Length: 336 bp
ATGATTAGTAAGGACGGAGAAAACCCAGGCCACGGTGGTCGCCACCTCACAACTCCTGCAGCCCTGAACCCAGCTGATTTCTATCCCATTTTTGTATTTGTTCCGTCAAAGGTGGACCTCCCCGGACTGAAGAAGGAAGAAGTGAAGGTTGAGCTGGAGGAAAGGAATGTCCTTCAGATTAGTGGAGAGAGAAGCAGGGAGCAGGAGGAGAAGAACGACAAGTGGCACAAGGTAGAGAGGAGCAGCAGCAAGTTCTTGAGGAGGTTCAGGCTGCCAGTACGTCGCCACCTCCACCCCACCAGAGCCTCGAGTTCACCGTCAAGTCGTTGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0016043 GO:0022607 GO:0043933 GO:0044085 GO:0050896 GO:0051259 GO:0065003 GO:0071840
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

111

Amino Acids

12.77

Weight (kDa)

9.92

Isoelectric Point (pI)

79.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 37 - 93 3e-13 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 2 cut(s) 149, 158
AfaI GTAC 1 cut(s) 279
AfiI CCNNNNNNNGG 1 cut(s) 32
AjnI CCWGG 1 cut(s) 25
AluBI AGCT 2 cut(s) 74, 154
AluI AGCT 2 cut(s) 74, 154
Ama87I CYCGRG 1 cut(s) 307
AoxI GGCC 1 cut(s) 28
ApeKI GCWGC 4 cut(s) 59, 243, 246, 271
AspS9I GGNCC 1 cut(s) 115
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 1 cut(s) 306
AvaI CYCGRG 1 cut(s) 307
AvaII GGWCC 1 cut(s) 115
BbvI GCAGC 4 cut(s) 71, 255, 258, 258
BciT130I CCWGG 1 cut(s) 27
BcnI CCSGG 1 cut(s) 123
BfmI CTRYAG 1 cut(s) 57
BisI GCNGC 4 cut(s) 60, 244, 247, 272
BlsI GCNGC 4 cut(s) 61, 245, 248, 273
Bme1390I CCNGG 2 cut(s) 27, 123
Bme18I GGWCC 1 cut(s) 115
BmeT110I CYCGRG 1 cut(s) 307
BmgT120I GGNCC 1 cut(s) 115
BmrFI CCNGG 2 cut(s) 27, 123
BpmI CTGGAG 1 cut(s) 176
BpuEI CTTGAG 1 cut(s) 277
BpuMI CCSGG 1 cut(s) 123
BsaBI GATNNNNATC 1 cut(s) 81
BsaJI CCNNGG 3 cut(s) 25, 31, 121
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 275
Bse8I GATNNNNATC 1 cut(s) 81
BseBI CCWGG 1 cut(s) 27
BseDI CCNNGG 3 cut(s) 25, 31, 121
BseJI GATNNNNATC 1 cut(s) 81
BseLI CCNNNNNNNGG 1 cut(s) 32
BseNI ACTGG 1 cut(s) 275
BseRI GAGGAG 3 cut(s) 221, 253, 274
BseXI GCAGC 4 cut(s) 71, 255, 258, 258
BseYI CCCAGC 1 cut(s) 70
BshFI GGCC 1 cut(s) 30
BsiHKCI CYCGRG 1 cut(s) 307
BsiSI CCGG 1 cut(s) 123
BslI CCNNNNNNNGG 1 cut(s) 32
BsnI GGCC 1 cut(s) 30
BsoBI CYCGRG 1 cut(s) 307
BspANI GGCC 1 cut(s) 30
BspMAI CTGCAG 1 cut(s) 61
BsrI ACTGG 1 cut(s) 275
BssECI CCNNGG 3 cut(s) 25, 31, 121
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 2 cut(s) 35, 318
BstDSI CCRYGG 1 cut(s) 31
BstNI CCWGG 1 cut(s) 27
BstSCI CCNGG 2 cut(s) 25, 121
BstSFI CTRYAG 1 cut(s) 57
BstV1I GCAGC 4 cut(s) 71, 255, 258, 258
BsuRI GGCC 1 cut(s) 30
BtgI CCRYGG 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 115
Csp6I GTAC 1 cut(s) 278
CviJI RGCY 6 cut(s) 30, 62, 74, 154, 271, 305
CviKI_1 RGCY 6 cut(s) 30, 62, 74, 154, 271, 305
CviQI GTAC 1 cut(s) 278
Eco47I GGWCC 1 cut(s) 115
Eco57I CTGAAG 2 cut(s) 149, 158
Eco88I CYCGRG 1 cut(s) 307
EcoRII CCWGG 1 cut(s) 25
Fnu4HI GCNGC 4 cut(s) 60, 244, 247, 272
Fsp4HI GCNGC 4 cut(s) 60, 244, 247, 272
GluI GCNGC 4 cut(s) 60, 244, 247, 272
GsaI CCCAGC 1 cut(s) 74
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 1 cut(s) 30
HapII CCGG 1 cut(s) 123
HpaII CCGG 1 cut(s) 123
HphI GGTGA 1 cut(s) 306
Hpy166II GTNNAC 2 cut(s) 115, 314
Hpy188I TCNGA 1 cut(s) 177
Hpy188III TCNNGA 1 cut(s) 256
Hpy8I GTNNAC 2 cut(s) 115, 314
Hpy99I CGWCG 1 cut(s) 285
HpyAV CCTTC 3 cut(s) 127, 139, 182
HpyCH4III ACNGT 2 cut(s) 35, 318
HpyCH4IV ACGT 1 cut(s) 280
HpyCH4V TGCA 1 cut(s) 59
HpySE526I ACGT 1 cut(s) 280
LmnI GCTCC 2 cut(s) 199, 240
Lsp1109I GCAGC 4 cut(s) 71, 255, 258, 258
MaeII ACGT 1 cut(s) 280
MboII GAAGA 3 cut(s) 142, 149, 223
MnlI CCTC 9 cut(s) 56, 128, 151, 199, 231, 252, 255, 299, 316
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 1 cut(s) 123
MspR9I CCNGG 2 cut(s) 27, 123
MvaI CCWGG 1 cut(s) 27
NciI CCSGG 1 cut(s) 123
PaeR7I CTCGAG 1 cut(s) 307
PcsI WCGNNNNNNNCGW 1 cut(s) 314
PkrI GCNGC 4 cut(s) 61, 245, 248, 273
Psp6I CCWGG 1 cut(s) 25
PspFI CCCAGC 1 cut(s) 70
PspGI CCWGG 1 cut(s) 25
PspPI GGNCC 1 cut(s) 115
PspXI VCTCGAGB 1 cut(s) 307
PstI CTGCAG 1 cut(s) 61
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 1 cut(s) 279
RsaNI GTAC 1 cut(s) 278
SatI GCNGC 4 cut(s) 60, 244, 247, 272
Sau96I GGNCC 1 cut(s) 115
ScrFI CCNGG 2 cut(s) 27, 123
SfcI CTRYAG 1 cut(s) 57
Sfr274I CTCGAG 1 cut(s) 307
SinI GGWCC 1 cut(s) 115
SlaI CTCGAG 1 cut(s) 307
SmlI CTYRAG 2 cut(s) 256, 307
SmoI CTYRAG 2 cut(s) 256, 307
StyD4I CCNGG 2 cut(s) 25, 121
TaaI ACNGT 2 cut(s) 35, 318
TaiI ACGT 1 cut(s) 283
TaqI TCGA 1 cut(s) 308
TseI GCWGC 4 cut(s) 59, 243, 246, 271
TspGWI ACGGA 2 cut(s) 30, 93
VpaK11BI GGWCC 1 cut(s) 115
XhoI CTCGAG 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.