Rmu_sc0001035.1_g000081

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001035.1
OG0010796
Physical Location & Seq
Forward (+)
397664 .. 401339
3676 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1317 bp
atgacagagctcgtcctccattctcatcctgaccatttctcttcatctccaacacccaccacctcttcctcttcttcttcttcttcttcttctccaccaccctccttaacctccgcaccgccgtcttctttaacctgcgcacccccgagggatgagtcttgggtgacggactgtgacgcccggcagcagctgtccctgttggcattgctggtcacgtttttcaggaagtctttgagtccttgcaagagggaaaggagggagctttgtgccatggagatcggctggcctaccaatgtgcgccacgtggcacatgtcacctttgacaggttcaatggcttcttgggtttgcctgttgagtttgagcctgaacttcccagtaggcctccgagtgcaagtttttgtgaggttgtctattttccctgttctgttagtttgcttaaacccagaaatgttatgaagtgcttcaatgaaatgttatgttctgtagaagggatattcagaattaatggagaaaacagtcaagaggagcatataagaggccagttaaacaggggagatgtaccagaaggcattgacatacactgcttggcagggctgatcaaggcttggtttagggaactcccagttgggcttctggattctctagcccctgagcaggtaatgcagtgccagacagaagacgactgcttagagcttgtgaggcttctaccctctaccgaagttgctctattggattgggcggccaaccttatggctgatgttgtcgaacacgaacatctaaacaagatgaatgcgcgcaacattgccatggtttttgcaccgaacatgactcagatggcagatcctttgactgcactgatgtatgctgttcaagtgatgaacttcctcaagattcttattctgaagacactgcgagaaaggaaagaaaatgtggtagaggccagtgtcttttgtgtagaacctgctgatgagaatggacaccagagctgttcgcaatcatgccatgaagatgttgtcgaagagagcctggttgagaacccaattcctaatggcagggactgctgtggtacttctgatcaagttgacactttgggtaatgaaatggaagctgtaaacaccatgacaactgaagttcaaggggactctgtgaagagcaaacctgatcaattgaataattcaactttcaaaatgggacctgaaagaataagtgaccagcaagctgcagtatggggaacagggcccgttgagaaggctaggaaaatcagcaacctgaatcgcatagattcaaggagggagctaattgaagcctggcggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

438

Amino Acids

48.71

Weight (kDa)

4.98

Isoelectric Point (pI)

49.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 153 - 278 4.7e-22 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 139
Acc36I ACCTGC 3 cut(s) 143, 646, 970
AccII CGCG 1 cut(s) 796
AciI CCGC 4 cut(s) 114, 119, 740, 1312
AclWI GGATC 1 cut(s) 836
AcoI YGGCCR 1 cut(s) 741
AcuI CTGAAG 2 cut(s) 923, 1149
AcvI CACGTG 1 cut(s) 304
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 2 cut(s) 561, 1069
AfiI CCNNNNNNNGG 2 cut(s) 324, 655
AflIII ACRYGT 1 cut(s) 310
AgsI TTSAA 9 cut(s) 331, 466, 872, 1136, 1171, 1179, 1186, 1287, 1304
AjnI CCWGG 2 cut(s) 1026, 1307
AjuI GAANNNNNNNTTGG 2 cut(s) 609, 641
AluBI AGCT 8 cut(s) 10, 190, 262, 694, 987, 1109, 1220, 1297
AluI AGCT 8 cut(s) 10, 190, 262, 694, 987, 1109, 1220, 1297
Alw21I GWGCWC 1 cut(s) 12
AlwI GGATC 1 cut(s) 836
AlwNI CAGNNNCTG 2 cut(s) 190, 1059
Ama87I CYCGRG 1 cut(s) 145
AoxI GGCC 6 cut(s) 284, 380, 538, 741, 939, 1238
ApaI GGGCCC 1 cut(s) 1242
ApeKI GCWGC 3 cut(s) 184, 187, 1220
AseI ATTAAT 1 cut(s) 504
Asp700I GAANNNNTTC 1 cut(s) 461
AspLEI GCGC 4 cut(s) 140, 300, 796, 798
AspS9I GGNCC 3 cut(s) 1193, 1238, 1239
AsuC2I CCSGG 1 cut(s) 181
AsuHPI GGTGA 2 cut(s) 175, 307
AvaI CYCGRG 1 cut(s) 145
AvaII GGWCC 1 cut(s) 1193
BaeGI GKGCMC 1 cut(s) 1242
BanII GRGCYC 2 cut(s) 12, 1242
BbrPI CACGTG 1 cut(s) 304
BbsI GAAGAC 3 cut(s) 117, 684, 911
Bbv12I GWGCWC 1 cut(s) 12
BbvI GCAGC 3 cut(s) 196, 199, 1207
BccI CCATC 1 cut(s) 829
BceAI ACGGC 1 cut(s) 106
BciT130I CCWGG 2 cut(s) 1028, 1309
BclI TGATCA 3 cut(s) 597, 1075, 1162
BcnI CCSGG 1 cut(s) 181
BfaI CTAG 2 cut(s) 644, 1254
BfmI CTRYAG 2 cut(s) 483, 1221
BfuAI ACCTGC 3 cut(s) 143, 646, 970
BisI GCNGC 4 cut(s) 185, 188, 741, 1221
BlsI GCNGC 4 cut(s) 186, 189, 742, 1222
Bme1390I CCNGG 3 cut(s) 181, 1028, 1309
Bme18I GGWCC 1 cut(s) 1193
BmeT110I CYCGRG 1 cut(s) 145
BmgT120I GGNCC 3 cut(s) 1193, 1238, 1239
BmiI GGNNCC 2 cut(s) 1194, 1240
BmrFI CCNGG 3 cut(s) 181, 1028, 1309
BmrI ACTGGG 2 cut(s) 369, 617
BmuI ACTGGG 2 cut(s) 369, 617
BpiI GAAGAC 3 cut(s) 117, 684, 911
Bpu10I CCTNAGC 1 cut(s) 651
BpuEI CTTGAG 1 cut(s) 872
BpuMI CCSGG 1 cut(s) 181
BsaAI YACGTR 1 cut(s) 304
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 3 cut(s) 146, 270, 807
Bsc4I CCNNNNNNNGG 2 cut(s) 324, 655
Bse1I ACTGG 4 cut(s) 375, 541, 623, 942
Bse3DI GCAATG 2 cut(s) 203, 801
BseBI CCWGG 2 cut(s) 1028, 1309
BseDI CCNNGG 3 cut(s) 146, 270, 807
BseGI GGATG 2 cut(s) 25, 157
BseLI CCNNNNNNNGG 2 cut(s) 324, 655
BseMI GCAATG 2 cut(s) 203, 801
BseMII CTCAG 2 cut(s) 642, 845
BseNI ACTGG 4 cut(s) 375, 541, 623, 942
BsePI GCGCGC 1 cut(s) 794
BseRI GAGGAG 1 cut(s) 539
BseSI GKGCMC 1 cut(s) 1242
BseXI GCAGC 3 cut(s) 196, 199, 1207
BsgI GTGCAG 1 cut(s) 837
Bsh1236I CGCG 1 cut(s) 796
BshFI GGCC 6 cut(s) 286, 382, 540, 743, 941, 1240
BsiHKAI GWGCWC 1 cut(s) 12
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 1 cut(s) 181
BslFI GGGAC 4 cut(s) 178, 1070, 1154, 1206
BslI CCNNNNNNNGG 2 cut(s) 324, 655
BsmFI GGGAC 4 cut(s) 178, 1070, 1154, 1206
BsmI GAATGC 1 cut(s) 796
BsnI GGCC 6 cut(s) 286, 382, 540, 743, 941, 1240
BsoBI CYCGRG 1 cut(s) 145
Bsp120I GGGCCC 1 cut(s) 1238
Bsp1286I GDGCHC 2 cut(s) 12, 1242
Bsp143I GATC 5 cut(s) 276, 597, 841, 1075, 1162
Bsp19I CCATGG 2 cut(s) 270, 807
BspACI CCGC 4 cut(s) 114, 119, 740, 1312
BspANI GGCC 6 cut(s) 286, 382, 540, 743, 941, 1240
BspCNI CTCAG 2 cut(s) 643, 844
BspFNI CGCG 1 cut(s) 796
BspLI GGNNCC 2 cut(s) 1194, 1240
BspMAI CTGCAG 1 cut(s) 1225
BspMI ACCTGC 3 cut(s) 143, 646, 970
BspPI GGATC 1 cut(s) 836
BspQI GCTCTTC 1 cut(s) 1145
BsrDI GCAATG 2 cut(s) 203, 801
BsrI ACTGG 4 cut(s) 375, 541, 623, 942
BssECI CCNNGG 3 cut(s) 146, 270, 807
BssHII GCGCGC 1 cut(s) 794
BssMI GATC 5 cut(s) 276, 597, 841, 1075, 1162
BssNI GRCGYC 1 cut(s) 177
BssT1I CCWWGG 2 cut(s) 270, 807
Bst2UI CCWGG 2 cut(s) 1028, 1309
Bst4CI ACNGT 2 cut(s) 173, 518
Bst6I CTCTTC 5 cut(s) 46, 70, 76, 1014, 1145
BstACI GRCGYC 1 cut(s) 177
BstAPI GCANNNNNTGC 2 cut(s) 661, 1059
BstBAI YACGTR 1 cut(s) 304
BstC8I GCNNGC 3 cut(s) 284, 796, 1218
BstDEI CTNAG 3 cut(s) 651, 688, 831
BstDSI CCRYGG 2 cut(s) 270, 807
BstENI CCTNNNNNAGG 1 cut(s) 322
BstF5I GGATG 2 cut(s) 25, 157
BstFNI CGCG 1 cut(s) 796
BstHHI GCGC 4 cut(s) 140, 300, 796, 798
BstKTI GATC 5 cut(s) 279, 600, 844, 1078, 1165
BstMBI GATC 5 cut(s) 276, 597, 841, 1075, 1162
BstMWI GCNNNNNNNGC 3 cut(s) 661, 700, 1059
BstNI CCWGG 2 cut(s) 1028, 1309
BstNSI RCATGY 1 cut(s) 314
BstSCI CCNGG 3 cut(s) 179, 1026, 1307
BstSFI CTRYAG 2 cut(s) 483, 1221
BstSLI GKGCMC 1 cut(s) 1242
BstUI CGCG 1 cut(s) 796
BstV1I GCAGC 3 cut(s) 196, 199, 1207
BstV2I GAAGAC 3 cut(s) 117, 684, 911
BstX2I RGATCY 1 cut(s) 841
BstXI CCANNNNNNTGG 1 cut(s) 751
BstYI RGATCY 1 cut(s) 841
BsuRI GGCC 6 cut(s) 286, 382, 540, 743, 941, 1240
BtgI CCRYGG 2 cut(s) 270, 807
BtsCI GGATG 2 cut(s) 25, 157
BtsI GCAGTG 3 cut(s) 580, 671, 908
BtsIMutI CAGTG 5 cut(s) 580, 671, 854, 908, 949
BveI ACCTGC 3 cut(s) 143, 646, 970
Cac8I GCNNGC 3 cut(s) 284, 796, 1218
CaiI CAGNNNCTG 2 cut(s) 190, 1059
CfoI GCGC 4 cut(s) 140, 300, 796, 798
Cfr13I GGNCC 3 cut(s) 1193, 1238, 1239
CseI GACGC 1 cut(s) 185
Csp6I GTAC 2 cut(s) 560, 1068
CviAII CATG 7 cut(s) 271, 311, 808, 826, 999, 1004, 1120
CviQI GTAC 2 cut(s) 560, 1068
DdeI CTNAG 3 cut(s) 651, 688, 831
DpnI GATC 5 cut(s) 278, 599, 843, 1077, 1164
DpnII GATC 5 cut(s) 276, 597, 841, 1075, 1162
EaeI YGGCCR 1 cut(s) 741
Eam1104I CTCTTC 5 cut(s) 46, 70, 76, 1014, 1145
EarI CTCTTC 5 cut(s) 46, 70, 76, 1014, 1145
Ecl136II GAGCTC 1 cut(s) 10
Eco130I CCWWGG 2 cut(s) 270, 807
Eco147I AGGCCT 1 cut(s) 382
Eco24I GRGCYC 2 cut(s) 12, 1242
Eco47I GGWCC 1 cut(s) 1193
Eco53kI GAGCTC 1 cut(s) 10
Eco57I CTGAAG 2 cut(s) 923, 1149
Eco72I CACGTG 1 cut(s) 304
Eco88I CYCGRG 1 cut(s) 145
EcoICRI GAGCTC 1 cut(s) 10
EcoNI CCTNNNNNAGG 1 cut(s) 322
EcoO109I RGGNCCY 2 cut(s) 1193, 1238
EcoRII CCWGG 2 cut(s) 1026, 1307
EcoT14I CCWWGG 2 cut(s) 270, 807
EcoT38I GRGCYC 2 cut(s) 12, 1242
ErhI CCWWGG 2 cut(s) 270, 807
FaeI CATG 7 cut(s) 274, 314, 811, 829, 1002, 1007, 1123
FaqI GGGAC 4 cut(s) 178, 1070, 1154, 1206
FatI CATG 7 cut(s) 270, 310, 807, 825, 998, 1003, 1119
FbaI TGATCA 3 cut(s) 597, 1075, 1162
Fnu4HI GCNGC 4 cut(s) 185, 188, 741, 1221
FokI GGATG 2 cut(s) 12, 164
FriOI GRGCYC 2 cut(s) 12, 1242
Fsp4HI GCNGC 4 cut(s) 185, 188, 741, 1221
FspBI CTAG 2 cut(s) 644, 1254
FspI TGCGCA 1 cut(s) 139
GlaI GCGC 4 cut(s) 139, 299, 795, 797
GluI GCNGC 4 cut(s) 185, 188, 741, 1221
HaeIII GGCC 6 cut(s) 286, 382, 540, 743, 941, 1240
HapII CCGG 1 cut(s) 181
HgaI GACGC 1 cut(s) 185
HhaI GCGC 4 cut(s) 140, 300, 796, 798
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 7 cut(s) 274, 314, 811, 829, 1002, 1007, 1123
Hin6I GCGC 4 cut(s) 138, 298, 794, 796
HinP1I GCGC 4 cut(s) 138, 298, 794, 796
HincII GTYRAC 1 cut(s) 1084
HindII GTYRAC 1 cut(s) 1084
HinfI GANTC 8 cut(s) 155, 235, 638, 829, 892, 1142, 1273, 1283
HpaII CCGG 1 cut(s) 181
HphI GGTGA 2 cut(s) 175, 307
Hpy166II GTNNAC 2 cut(s) 1084, 1114
Hpy188I TCNGA 5 cut(s) 387, 500, 834, 903, 1075
Hpy188III TCNNGA 5 cut(s) 29, 223, 521, 635, 889
Hpy8I GTNNAC 2 cut(s) 1084, 1114
HpyAV CCTTC 3 cut(s) 482, 560, 1243
HpyCH4III ACNGT 2 cut(s) 173, 518
HpyCH4IV ACGT 2 cut(s) 215, 303
HpyCH4V TGCA 6 cut(s) 243, 392, 664, 818, 854, 1223
HpyF10VI GCNNNNNNNGC 3 cut(s) 661, 700, 1059
HpyF3I CTNAG 3 cut(s) 651, 688, 831
HpySE526I ACGT 2 cut(s) 215, 303
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 7 cut(s) 274, 314, 811, 829, 1002, 1007, 1123
HspAI GCGC 4 cut(s) 138, 298, 794, 796
Ksp22I TGATCA 3 cut(s) 597, 1075, 1162
Kzo9I GATC 5 cut(s) 276, 597, 841, 1075, 1162
LguI GCTCTTC 1 cut(s) 1145
LmnI GCTCC 3 cut(s) 259, 526, 1294
Lsp1109I GCAGC 3 cut(s) 196, 199, 1207
MaeI CTAG 2 cut(s) 644, 1254
MaeII ACGT 2 cut(s) 215, 303
MaeIII GTNAC 5 cut(s) 163, 173, 211, 313, 1208
MalI GATC 5 cut(s) 278, 599, 843, 1077, 1164
MboI GATC 5 cut(s) 276, 597, 841, 1075, 1162
MfeI CAATTG 1 cut(s) 1166
MflI RGATCY 1 cut(s) 841
MhlI GDGCHC 2 cut(s) 12, 1242
MluCI AATT 5 cut(s) 501, 1041, 1166, 1174, 1299
MlyI GAGTC 4 cut(s) 164, 244, 823, 1136
MmeI TCCRAC 1 cut(s) 74
MroXI GAANNNNTTC 1 cut(s) 461
MseI TTAA 5 cut(s) 107, 131, 438, 504, 545
MslI CAYNNNNRTG 2 cut(s) 806, 1008
MspA1I CMGCKG 1 cut(s) 190
MspI CCGG 1 cut(s) 181
MspR9I CCNGG 3 cut(s) 181, 1028, 1309
MunI CAATTG 1 cut(s) 1166
Mva1269I GAATGC 1 cut(s) 796
MvaI CCWGG 2 cut(s) 1028, 1309
MvnI CGCG 1 cut(s) 796
MwoI GCNNNNNNNGC 3 cut(s) 661, 700, 1059
NciI CCSGG 1 cut(s) 181
NcoI CCATGG 2 cut(s) 270, 807
NdeII GATC 5 cut(s) 276, 597, 841, 1075, 1162
NlaIII CATG 7 cut(s) 274, 314, 811, 829, 1002, 1007, 1123
NlaIV GGNNCC 2 cut(s) 1194, 1240
NmuCI GTSAC 5 cut(s) 163, 173, 211, 313, 1208
NsbI TGCGCA 1 cut(s) 139
NspI RCATGY 1 cut(s) 314
PauI GCGCGC 1 cut(s) 794
PceI AGGCCT 1 cut(s) 382
PciI ACATGT 1 cut(s) 310
PciSI GCTCTTC 1 cut(s) 1145
PctI GAATGC 1 cut(s) 796
PdmI GAANNNNTTC 1 cut(s) 461
PfeI GAWTC 4 cut(s) 638, 892, 1273, 1283
PkrI GCNGC 4 cut(s) 186, 189, 742, 1222
PleI GAGTC 4 cut(s) 163, 243, 823, 1136
PmaCI CACGTG 1 cut(s) 304
PmlI CACGTG 1 cut(s) 304
PpsI GAGTC 4 cut(s) 163, 243, 823, 1136
Ppu21I YACGTR 1 cut(s) 304
PpuMI RGGWCCY 1 cut(s) 1193
PscI ACATGT 1 cut(s) 310
PshBI ATTAAT 1 cut(s) 504
Psp124BI GAGCTC 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 1193
Psp6I CCWGG 2 cut(s) 1026, 1307
PspCI CACGTG 1 cut(s) 304
PspGI CCWGG 2 cut(s) 1026, 1307
PspN4I GGNNCC 2 cut(s) 1194, 1240
PspOMI GGGCCC 1 cut(s) 1238
PspPI GGNCC 3 cut(s) 1193, 1238, 1239
PspPPI RGGWCCY 1 cut(s) 1193
PstI CTGCAG 1 cut(s) 1225
PstNI CAGNNNCTG 2 cut(s) 190, 1059
PsuI RGATCY 1 cut(s) 841
PteI GCGCGC 1 cut(s) 794
PvuII CAGCTG 1 cut(s) 190
RsaI GTAC 2 cut(s) 561, 1069
RsaNI GTAC 2 cut(s) 560, 1068
RseI CAYNNNNRTG 2 cut(s) 806, 1008
SacI GAGCTC 1 cut(s) 12
SapI GCTCTTC 1 cut(s) 1145
SaqAI TTAA 5 cut(s) 107, 131, 438, 504, 545
SatI GCNGC 4 cut(s) 185, 188, 741, 1221
Sau3AI GATC 5 cut(s) 276, 597, 841, 1075, 1162
Sau96I GGNCC 3 cut(s) 1193, 1238, 1239
SchI GAGTC 4 cut(s) 164, 244, 823, 1136
ScrFI CCNGG 3 cut(s) 181, 1028, 1309
SduI GDGCHC 2 cut(s) 12, 1242
SfcI CTRYAG 2 cut(s) 483, 1221
SinI GGWCC 1 cut(s) 1193
SmiMI CAYNNNNRTG 2 cut(s) 806, 1008
SmlI CTYRAG 1 cut(s) 887
SmoI CTYRAG 1 cut(s) 887
Sse9I AATT 5 cut(s) 501, 1041, 1166, 1174, 1299
SseBI AGGCCT 1 cut(s) 382
SsiI CCGC 4 cut(s) 114, 119, 740, 1312
SspMI CTAG 2 cut(s) 644, 1254
SstI GAGCTC 1 cut(s) 12
StuI AGGCCT 1 cut(s) 382
StyD4I CCNGG 3 cut(s) 179, 1026, 1307
StyI CCWWGG 2 cut(s) 270, 807
TaaI ACNGT 2 cut(s) 173, 518
TaiI ACGT 2 cut(s) 218, 306
TaqI TCGA 2 cut(s) 765, 1017
TasI AATT 5 cut(s) 501, 1041, 1166, 1174, 1299
TauI GCSGC 1 cut(s) 743
TfiI GAWTC 4 cut(s) 638, 892, 1273, 1283
Tru1I TTAA 5 cut(s) 107, 131, 438, 504, 545
Tru9I TTAA 5 cut(s) 107, 131, 438, 504, 545
TscAI CASTG 5 cut(s) 587, 671, 861, 915, 949
TseFI GTSAC 5 cut(s) 163, 173, 211, 313, 1208
TseI GCWGC 3 cut(s) 184, 187, 1220
Tsp45I GTSAC 5 cut(s) 163, 173, 211, 313, 1208
TspDTI ATGAA 7 cut(s) 33, 470, 483, 803, 893, 1020, 1113
TspGWI ACGGA 1 cut(s) 182
TspRI CASTG 5 cut(s) 587, 671, 861, 915, 949
VpaK11BI GGWCC 1 cut(s) 1193
VspI ATTAAT 1 cut(s) 504
XagI CCTNNNNNAGG 1 cut(s) 322
XceI RCATGY 1 cut(s) 314
XmnI GAANNNNTTC 1 cut(s) 461
XspI CTAG 2 cut(s) 644, 1254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.