Rmu_sc0001299.1_g000022

Monothiol glutaredoxin-S15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001299.1
OG0011348
Physical Location & Seq
Reverse (-)
127322 .. 131993
4672 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1095 bp
atggtgaaccggtggaatatttgctgcgggttggatggagcttccggtggccagttcggtaggtttccgaccagttcttggggtggctcctccggttctggactcctgagatcgaatgcttcaaggtgtgaggtggaggcagaaaaggcttcaaggttttgtattcggattcaagaccagacaaaggcgatggacggcggcgtgggcttggggagactgtggcttcttctgcgacggcattgggctctcgaccaaggttttcgaaggcttcggagggcaacgatgattgggccggtggttgagactagtttgatggaggtcagtgcggcggaaggctacgggcaggaagaaggtccggaaagatgcatgggaaaccctagttttgaactagggttgattgccttgaccctcaagaagagcctgaagagcaacctgttgccgatgtttttggctactgaggaggctgaagatggttcaatcgaacagaagtacatagcagacggacagcagccagttccaggcggaaaagtactgttggttcttccttccatcttcttcttcttcttcttcttcagcatggctaggtctatttcaaatttgctcttcaagggtctctcattttcaagcgtcccagcttctcgttcttccacaattgctcctgggctactttatctcaatggaatgagatactccacctctgtgccgaatgatcccgatacgcatgatgactttaaacccacggataagtttaagggttctggccttagcttgaaggacatcgttgaacgggatgtcaaggaaaaccctgtaatgctttatatgaaaggagtgcctgattctcctcaatgcggattcagctcacttgcagtgagagtgttatcactataccatgttcctttgagtgctagaaatatcttggaagatcctgagctgaaaaatgctgtgaaagcctatagcacctggccaacatttccacagattttcatcaagggggagtttattggaggttcagatattattctcaatatgcatcagaacggtgagcttaaggaaaagcttaaggatattgcagccaatcaggagaagtctgaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

364

Amino Acids

40.05

Weight (kDa)

7.58

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 270 - 334 5.7e-17 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 78
AccIII TCCGGA 1 cut(s) 355
AciI CCGC 6 cut(s) 27, 198, 326, 329, 522, 849
AclWI GGATC 2 cut(s) 704, 917
AcoI YGGCCR 2 cut(s) 49, 962
AcsI RAATTY 1 cut(s) 595
AcuI CTGAAG 3 cut(s) 443, 486, 556
AfaI GTAC 2 cut(s) 491, 531
AfiI CCNNNNNNNGG 4 cut(s) 78, 184, 518, 848
AflII CTTAAG 2 cut(s) 1046, 1058
AgeI ACCGGT 1 cut(s) 9
AhlI ACTAGT 1 cut(s) 305
AjnI CCWGG 3 cut(s) 517, 658, 959
AleI CACNNNNGTG 1 cut(s) 698
AluBI AGCT 7 cut(s) 41, 635, 768, 858, 931, 1045, 1057
AluI AGCT 7 cut(s) 41, 635, 768, 858, 931, 1045, 1057
Alw26I GTCTC 3 cut(s) 208, 296, 617
AlwI GGATC 2 cut(s) 704, 917
Aor13HI TCCGGA 1 cut(s) 355
AoxI GGCC 4 cut(s) 49, 290, 760, 962
ApeKI GCWGC 3 cut(s) 24, 508, 1070
ApoI RAATTY 1 cut(s) 595
AsiGI ACCGGT 1 cut(s) 9
AspS9I GGNCC 2 cut(s) 290, 353
AsuHPI GGTGA 2 cut(s) 16, 1052
AsuII TTCGAA 1 cut(s) 262
AvaII GGWCC 1 cut(s) 353
BalI TGGCCA 2 cut(s) 51, 964
BanII GRGCYC 1 cut(s) 247
BbvI GCAGC 3 cut(s) 11, 520, 1082
BccI CCATC 5 cut(s) 29, 184, 307, 464, 557
BceAI ACGGC 2 cut(s) 211, 251
BciT130I CCWGG 3 cut(s) 519, 660, 961
BcoDI GTCTC 3 cut(s) 208, 296, 617
BcuI ACTAGT 1 cut(s) 305
BfaI CTAG 5 cut(s) 306, 378, 389, 582, 906
BfmI CTRYAG 1 cut(s) 952
BfrI CTTAAG 2 cut(s) 1046, 1058
BisI GCNGC 5 cut(s) 25, 199, 327, 509, 1071
BlsI GCNGC 5 cut(s) 26, 200, 328, 510, 1072
BmcAI AGTACT 1 cut(s) 531
Bme1390I CCNGG 3 cut(s) 519, 660, 961
Bme18I GGWCC 1 cut(s) 353
BmgT120I GGNCC 2 cut(s) 290, 353
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 3 cut(s) 519, 660, 961
BmsI GCATC 2 cut(s) 353, 1039
Bpu10I CCTNAGC 2 cut(s) 764, 927
Bpu14I TTCGAA 1 cut(s) 262
BpuEI CTTGAG 1 cut(s) 395
BsaBI GATNNNNATC 1 cut(s) 983
BsaI GGTCTC 1 cut(s) 617
BsaJI CCNNGG 3 cut(s) 253, 659, 738
BsaWI WCCGGW 4 cut(s) 9, 44, 92, 355
BsaXI ACNNNNNCTCC 2 cut(s) 640, 670
Bsc4I CCNNNNNNNGG 4 cut(s) 78, 184, 518, 848
Bse118I RCCGGY 2 cut(s) 9, 292
Bse1I ACTGG 3 cut(s) 52, 72, 512
Bse8I GATNNNNATC 1 cut(s) 983
BseAI TCCGGA 1 cut(s) 355
BseBI CCWGG 3 cut(s) 519, 660, 961
BseDI CCNNGG 3 cut(s) 253, 659, 738
BseGI GGATG 2 cut(s) 40, 796
BseJI GATNNNNATC 1 cut(s) 983
BseLI CCNNNNNNNGG 4 cut(s) 78, 184, 518, 848
BseMII CTCAG 3 cut(s) 98, 447, 918
BseNI ACTGG 3 cut(s) 52, 72, 512
BseRI GAGGAG 3 cut(s) 79, 473, 831
BseXI GCAGC 3 cut(s) 11, 520, 1082
BseYI CCCAGC 1 cut(s) 631
BshFI GGCC 4 cut(s) 51, 292, 762, 964
BshTI ACCGGT 1 cut(s) 9
BsiSI CCGG 5 cut(s) 10, 45, 93, 293, 356
BslFI GGGAC 1 cut(s) 614
BslI CCNNNNNNNGG 4 cut(s) 78, 184, 518, 848
BsmAI GTCTC 3 cut(s) 208, 296, 617
BsmFI GGGAC 1 cut(s) 614
BsmI GAATGC 1 cut(s) 121
BsnI GGCC 4 cut(s) 51, 292, 762, 964
Bso31I GGTCTC 1 cut(s) 617
Bsp119I TTCGAA 1 cut(s) 262
Bsp1286I GDGCHC 1 cut(s) 247
Bsp13I TCCGGA 1 cut(s) 355
Bsp143I GATC 3 cut(s) 110, 709, 922
BspACI CCGC 6 cut(s) 27, 198, 326, 329, 522, 849
BspANI GGCC 4 cut(s) 51, 292, 762, 964
BspCNI CTCAG 3 cut(s) 99, 448, 919
BspEI TCCGGA 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 88
BspPI GGATC 2 cut(s) 704, 917
BspQI GCTCTTC 3 cut(s) 410, 419, 608
BspT104I TTCGAA 1 cut(s) 262
BspTI CTTAAG 2 cut(s) 1046, 1058
BspTNI GGTCTC 1 cut(s) 617
BsrFI RCCGGY 2 cut(s) 9, 292
BsrI ACTGG 3 cut(s) 52, 72, 512
BssAI RCCGGY 2 cut(s) 9, 292
BssECI CCNNGG 3 cut(s) 253, 659, 738
BssMI GATC 3 cut(s) 110, 709, 922
BssT1I CCWWGG 1 cut(s) 253
Bst2UI CCWGG 3 cut(s) 519, 660, 961
Bst4CI ACNGT 3 cut(s) 219, 534, 1040
Bst6I CTCTTC 3 cut(s) 410, 419, 608
BstAFI CTTAAG 2 cut(s) 1046, 1058
BstBI TTCGAA 1 cut(s) 262
BstDEI CTNAG 4 cut(s) 107, 456, 764, 927
BstDSI CCRYGG 1 cut(s) 738
BstF5I GGATG 2 cut(s) 40, 796
BstKTI GATC 3 cut(s) 113, 712, 925
BstMAI GTCTC 3 cut(s) 208, 296, 617
BstMBI GATC 3 cut(s) 110, 709, 922
BstMWI GCNNNNNNNGC 6 cut(s) 146, 204, 229, 426, 855, 947
BstNI CCWGG 3 cut(s) 519, 660, 961
BstSCI CCNGG 3 cut(s) 517, 658, 959
BstSFI CTRYAG 1 cut(s) 952
BstV1I GCAGC 3 cut(s) 11, 520, 1082
BstX2I RGATCY 1 cut(s) 922
BstYI RGATCY 1 cut(s) 922
BsuRI GGCC 4 cut(s) 51, 292, 762, 964
BtgI CCRYGG 1 cut(s) 738
BtgZI GCGATG 1 cut(s) 203
BtsCI GGATG 2 cut(s) 40, 796
BtsI GCAGTG 1 cut(s) 873
BtsIMutI CAGTG 2 cut(s) 328, 873
Cfr10I RCCGGY 2 cut(s) 9, 292
Cfr13I GGNCC 2 cut(s) 290, 353
CseI GACGC 1 cut(s) 616
Csp6I GTAC 2 cut(s) 490, 530
CspAI ACCGGT 1 cut(s) 9
CviAII CATG 4 cut(s) 367, 577, 722, 890
CviQI GTAC 2 cut(s) 490, 530
DdeI CTNAG 4 cut(s) 107, 456, 764, 927
DpnI GATC 3 cut(s) 112, 711, 924
DpnII GATC 3 cut(s) 110, 709, 922
DraI TTTAAA 1 cut(s) 733
EaeI YGGCCR 2 cut(s) 49, 962
Eam1104I CTCTTC 3 cut(s) 410, 419, 608
EarI CTCTTC 3 cut(s) 410, 419, 608
EciI GGCGGA 2 cut(s) 344, 537
Eco130I CCWWGG 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 247
Eco31I GGTCTC 1 cut(s) 617
Eco47I GGWCC 1 cut(s) 353
Eco57I CTGAAG 3 cut(s) 443, 486, 556
EcoRII CCWGG 3 cut(s) 517, 658, 959
EcoT14I CCWWGG 1 cut(s) 253
EcoT22I ATGCAT 2 cut(s) 368, 1032
EcoT38I GRGCYC 1 cut(s) 247
ErhI CCWWGG 1 cut(s) 253
FaeI CATG 4 cut(s) 370, 580, 725, 893
FaqI GGGAC 1 cut(s) 614
FatI CATG 4 cut(s) 366, 576, 721, 889
FauI CCCGC 1 cut(s) 20
Fnu4HI GCNGC 5 cut(s) 25, 199, 327, 509, 1071
FokI GGATG 2 cut(s) 47, 803
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 5 cut(s) 25, 199, 327, 509, 1071
FspBI CTAG 5 cut(s) 306, 378, 389, 582, 906
GluI GCNGC 5 cut(s) 25, 199, 327, 509, 1071
GsaI CCCAGC 1 cut(s) 635
HaeIII GGCC 4 cut(s) 51, 292, 762, 964
HapII CCGG 5 cut(s) 10, 45, 93, 293, 356
HgaI GACGC 1 cut(s) 616
Hin1II CATG 4 cut(s) 370, 580, 725, 893
HindIII AAGCTT 1 cut(s) 1055
HinfI GANTC 4 cut(s) 102, 169, 836, 852
HpaII CCGG 5 cut(s) 10, 45, 93, 293, 356
HphI GGTGA 2 cut(s) 16, 1052
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 6 cut(s) 69, 168, 273, 1012, 1035, 1090
Hpy188III TCNNGA 9 cut(s) 99, 106, 173, 248, 356, 412, 713, 926, 1079
Hpy8I GTNNAC 1 cut(s) 7
Hpy99I CGWCG 1 cut(s) 237
HpyAV CCTTC 5 cut(s) 258, 326, 344, 555, 766
HpyCH4III ACNGT 3 cut(s) 219, 534, 1040
HpyCH4V TGCA 4 cut(s) 366, 866, 1030, 1070
HpyF10VI GCNNNNNNNGC 6 cut(s) 146, 204, 229, 426, 855, 947
HpyF3I CTNAG 4 cut(s) 107, 456, 764, 927
Hsp92II CATG 4 cut(s) 370, 580, 725, 893
Kpn2I TCCGGA 1 cut(s) 355
Kzo9I GATC 3 cut(s) 110, 709, 922
LguI GCTCTTC 3 cut(s) 410, 419, 608
LmnI GCTCC 3 cut(s) 38, 92, 661
Lsp1109I GCAGC 3 cut(s) 11, 520, 1082
LweI GCATC 2 cut(s) 353, 1039
MaeI CTAG 5 cut(s) 306, 378, 389, 582, 906
MalI GATC 3 cut(s) 112, 711, 924
MboI GATC 3 cut(s) 110, 709, 922
MfeI CAATTG 1 cut(s) 651
MflI RGATCY 1 cut(s) 922
MhlI GDGCHC 1 cut(s) 247
MlsI TGGCCA 2 cut(s) 51, 964
MluCI AATT 2 cut(s) 595, 651
MluNI TGGCCA 2 cut(s) 51, 964
MlyI GAGTC 1 cut(s) 96
MmeI TCCRAC 2 cut(s) 12, 92
Mox20I TGGCCA 2 cut(s) 51, 964
Mph1103I ATGCAT 2 cut(s) 368, 1032
MroI TCCGGA 1 cut(s) 355
MscI TGGCCA 2 cut(s) 51, 964
MseI TTAA 4 cut(s) 732, 750, 1047, 1059
MslI CAYNNNNRTG 1 cut(s) 698
Msp20I TGGCCA 2 cut(s) 51, 964
MspCI CTTAAG 2 cut(s) 1046, 1058
MspI CCGG 5 cut(s) 10, 45, 93, 293, 356
MspR9I CCNGG 3 cut(s) 519, 660, 961
MunI CAATTG 1 cut(s) 651
Mva1269I GAATGC 1 cut(s) 121
MvaI CCWGG 3 cut(s) 519, 660, 961
MwoI GCNNNNNNNGC 6 cut(s) 146, 204, 229, 426, 855, 947
NdeII GATC 3 cut(s) 110, 709, 922
NlaIII CATG 4 cut(s) 370, 580, 725, 893
NlaIV GGNNCC 1 cut(s) 88
NsiI ATGCAT 2 cut(s) 368, 1032
NspV TTCGAA 1 cut(s) 262
OliI CACNNNNGTG 1 cut(s) 698
PciSI GCTCTTC 3 cut(s) 410, 419, 608
PctI GAATGC 1 cut(s) 121
PfeI GAWTC 3 cut(s) 169, 836, 852
PflMI CCANNNNNTGG 1 cut(s) 78
PinAI ACCGGT 1 cut(s) 9
PkrI GCNGC 5 cut(s) 26, 200, 328, 510, 1072
PleI GAGTC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 96
Psp6I CCWGG 3 cut(s) 517, 658, 959
PspFI CCCAGC 1 cut(s) 631
PspGI CCWGG 3 cut(s) 517, 658, 959
PspN4I GGNNCC 1 cut(s) 88
PspPI GGNCC 2 cut(s) 290, 353
PsrI GAACNNNNNNTAC 2 cut(s) 522, 554
PsuI RGATCY 1 cut(s) 922
RsaI GTAC 2 cut(s) 491, 531
RsaNI GTAC 2 cut(s) 490, 530
RseI CAYNNNNRTG 1 cut(s) 698
SapI GCTCTTC 3 cut(s) 410, 419, 608
SaqAI TTAA 4 cut(s) 732, 750, 1047, 1059
SatI GCNGC 5 cut(s) 25, 199, 327, 509, 1071
Sau3AI GATC 3 cut(s) 110, 709, 922
Sau96I GGNCC 2 cut(s) 290, 353
ScaI AGTACT 1 cut(s) 531
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 3 cut(s) 519, 660, 961
SduI GDGCHC 1 cut(s) 247
SfaNI GCATC 2 cut(s) 353, 1039
SfcI CTRYAG 1 cut(s) 952
SfuI TTCGAA 1 cut(s) 262
SinI GGWCC 1 cut(s) 353
SmiMI CAYNNNNRTG 1 cut(s) 698
SmlI CTYRAG 3 cut(s) 410, 1046, 1058
SmoI CTYRAG 3 cut(s) 410, 1046, 1058
SpeI ACTAGT 1 cut(s) 305
Sse9I AATT 2 cut(s) 595, 651
SsiI CCGC 6 cut(s) 27, 198, 326, 329, 522, 849
SspI AATATT 1 cut(s) 19
SspMI CTAG 5 cut(s) 306, 378, 389, 582, 906
StyD4I CCNGG 3 cut(s) 517, 658, 959
StyI CCWWGG 1 cut(s) 253
TaaI ACNGT 3 cut(s) 219, 534, 1040
TaqI TCGA 4 cut(s) 113, 249, 262, 480
TasI AATT 2 cut(s) 595, 651
TatI WGTACW 2 cut(s) 489, 529
TauI GCSGC 2 cut(s) 201, 329
TfiI GAWTC 3 cut(s) 169, 836, 852
Tru1I TTAA 4 cut(s) 732, 750, 1047, 1059
Tru9I TTAA 4 cut(s) 732, 750, 1047, 1059
TscAI CASTG 2 cut(s) 328, 873
TseI GCWGC 3 cut(s) 24, 508, 1070
TspDTI ATGAA 2 cut(s) 836, 973
TspGWI ACGGA 2 cut(s) 516, 755
TspRI CASTG 2 cut(s) 328, 873
Van91I CCANNNNNTGG 1 cut(s) 78
Vha464I CTTAAG 2 cut(s) 1046, 1058
VpaK11BI GGWCC 1 cut(s) 353
XapI RAATTY 1 cut(s) 595
XspI CTAG 5 cut(s) 306, 378, 389, 582, 906
ZrmI AGTACT 1 cut(s) 531
Zsp2I ATGCAT 2 cut(s) 368, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.