Rmu_sc0001460.1_g000008

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001460.1
OG0010635
Physical Location & Seq
Reverse (-)
41354 .. 43974
2621 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
atgtttatcattaatgctggttctggttttagaatgttgtggagcactgtgaagtccttccttgatcccaagacaactgaaaagattaatgtccttggtgacaagagcaagttacttcaaataattgatgctagatggcttttcttggcgtgtcaccctcgcttgtggctgcgagttgaacgatccattaaagatccattggagcctggcgtcttccccagcattgagacagcggttgctgccgcaaggcttggcgacaccattctaatttcagcagggggaaagcatcttgcccccccccccccccccccccatgcattggagttcctgtccacctgcaaattggcaaacttaactgtaaaggcagagccgggttgttgcttgcttcatcgaagtggaagactcacaatagatgggtgcgtgcttcagtgccagacaaatcccctagacttcctttcagtccccattgtgagtacggcaagtgcgcaaactctgttgtcttcatcggtgaagtgttataaggatggtgtttctgtttctctgactcaaattggaggtgccaactgccaaggctgtttcaactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

194

Amino Acids

20.87

Weight (kDa)

8.77

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 1 - 44 1.5e-07 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 519
AarI CACCTGC 1 cut(s) 344
Acc16I TGCGCA 1 cut(s) 486
Acc36I ACCTGC 1 cut(s) 344
AccB1I GGYRCC 1 cut(s) 557
AccB7I CCANNNNNTGG 1 cut(s) 319
AciI CCGC 2 cut(s) 233, 243
AclWI GGATC 3 cut(s) 59, 177, 188
AcuI CTGAAG 1 cut(s) 410
AcyI GRCGYC 1 cut(s) 210
AfaI GTAC 1 cut(s) 475
AfiI CCNNNNNNNGG 1 cut(s) 319
AgsI TTSAA 3 cut(s) 119, 179, 580
AjnI CCWGG 1 cut(s) 205
Alw21I GWGCWC 1 cut(s) 47
Alw26I GTCTC 1 cut(s) 221
AlwI GGATC 3 cut(s) 59, 177, 188
ApeKI GCWGC 2 cut(s) 169, 239
AseI ATTAAT 2 cut(s) 12, 87
Asp700I GAANNNNTTC 1 cut(s) 56
AspLEI GCGC 1 cut(s) 487
AsuC2I CCSGG 1 cut(s) 372
AsuHPI GGTGA 3 cut(s) 110, 146, 520
BanI GGYRCC 1 cut(s) 557
BbsI GAAGAC 3 cut(s) 205, 406, 492
Bbv12I GWGCWC 1 cut(s) 47
BbvI GCAGC 2 cut(s) 156, 226
BccI CCATC 3 cut(s) 129, 407, 518
BceAI ACGGC 1 cut(s) 492
BciT130I CCWGG 1 cut(s) 207
BcnI CCSGG 1 cut(s) 372
BcoDI GTCTC 1 cut(s) 221
BfaI CTAG 3 cut(s) 132, 446, 583
BfuAI ACCTGC 1 cut(s) 344
BisI GCNGC 3 cut(s) 170, 240, 243
BlsI GCNGC 3 cut(s) 171, 241, 244
Bme1390I CCNGG 2 cut(s) 207, 372
BmiI GGNNCC 2 cut(s) 204, 559
BmrFI CCNGG 2 cut(s) 207, 372
BmsI GCATC 2 cut(s) 118, 295
BpiI GAAGAC 3 cut(s) 205, 406, 492
BpuMI CCSGG 1 cut(s) 372
BsaHI GRCGYC 1 cut(s) 210
BsaJI CCNNGG 2 cut(s) 94, 568
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 1 cut(s) 319
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 94, 568
BseGI GGATG 1 cut(s) 529
BseLI CCNNNNNNNGG 1 cut(s) 319
BseXI GCAGC 2 cut(s) 156, 226
BseYI CCCAGC 1 cut(s) 218
BshNI GGYRCC 1 cut(s) 557
BsiHKAI GWGCWC 1 cut(s) 47
BsiSI CCGG 1 cut(s) 371
BslFI GGGAC 1 cut(s) 446
BslI CCNNNNNNNGG 1 cut(s) 319
BsmAI GTCTC 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 446
Bsp1286I GDGCHC 1 cut(s) 47
Bsp143I GATC 3 cut(s) 64, 182, 193
BspACI CCGC 2 cut(s) 233, 243
BspLI GGNNCC 2 cut(s) 204, 559
BspMI ACCTGC 1 cut(s) 344
BspPI GGATC 3 cut(s) 59, 177, 188
BspT107I GGYRCC 1 cut(s) 557
BssECI CCNNGG 2 cut(s) 94, 568
BssMI GATC 3 cut(s) 64, 182, 193
BssNI GRCGYC 1 cut(s) 210
BssT1I CCWWGG 2 cut(s) 94, 568
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 2 cut(s) 49, 358
BstACI GRCGYC 1 cut(s) 210
BstC8I GCNNGC 2 cut(s) 383, 422
BstF5I GGATG 1 cut(s) 529
BstHHI GCGC 1 cut(s) 487
BstKTI GATC 3 cut(s) 67, 185, 196
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 3 cut(s) 64, 182, 193
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNI CCWGG 1 cut(s) 207
BstSCI CCNGG 2 cut(s) 205, 370
BstV1I GCAGC 2 cut(s) 156, 226
BstV2I GAAGAC 3 cut(s) 205, 406, 492
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BtsCI GGATG 1 cut(s) 529
BtsIMutI CAGTG 2 cut(s) 45, 434
BveI ACCTGC 1 cut(s) 344
Cac8I GCNNGC 2 cut(s) 383, 422
CfoI GCGC 1 cut(s) 487
CseI GACGC 1 cut(s) 199
Csp6I GTAC 1 cut(s) 474
CviAII CATG 1 cut(s) 314
CviJI RGCY 6 cut(s) 139, 169, 205, 250, 370, 573
CviKI_1 RGCY 6 cut(s) 139, 169, 205, 250, 370, 573
CviQI GTAC 1 cut(s) 474
DpnI GATC 3 cut(s) 66, 184, 195
DpnII GATC 3 cut(s) 64, 182, 193
Eco130I CCWWGG 2 cut(s) 94, 568
Eco57I CTGAAG 1 cut(s) 410
EcoRII CCWGG 1 cut(s) 205
EcoT14I CCWWGG 2 cut(s) 94, 568
EcoT22I ATGCAT 1 cut(s) 319
ErhI CCWWGG 2 cut(s) 94, 568
FaeI CATG 1 cut(s) 317
FaiI YATR 2 cut(s) 315, 519
FaqI GGGAC 1 cut(s) 446
FatI CATG 1 cut(s) 313
Fnu4HI GCNGC 3 cut(s) 170, 240, 243
FokI GGATG 1 cut(s) 536
Fsp4HI GCNGC 3 cut(s) 170, 240, 243
FspBI CTAG 3 cut(s) 132, 446, 583
FspI TGCGCA 1 cut(s) 486
GlaI GCGC 1 cut(s) 486
GluI GCNGC 3 cut(s) 170, 240, 243
GsaI CCCAGC 1 cut(s) 222
HapII CCGG 1 cut(s) 371
HgaI GACGC 1 cut(s) 199
HhaI GCGC 1 cut(s) 487
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 1 cut(s) 317
Hin6I GCGC 1 cut(s) 485
HinP1I GCGC 1 cut(s) 485
HinfI GANTC 2 cut(s) 402, 544
HpaII CCGG 1 cut(s) 371
HphI GGTGA 3 cut(s) 110, 146, 520
Hpy166II GTNNAC 1 cut(s) 333
Hpy188I TCNGA 1 cut(s) 543
Hpy8I GTNNAC 1 cut(s) 333
HpyAV CCTTC 1 cut(s) 67
HpyCH4III ACNGT 2 cut(s) 49, 358
HpyCH4V TGCA 2 cut(s) 317, 339
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 1 cut(s) 317
HspAI GCGC 1 cut(s) 485
Kzo9I GATC 3 cut(s) 64, 182, 193
LmnI GCTCC 2 cut(s) 42, 202
Lsp1109I GCAGC 2 cut(s) 156, 226
LweI GCATC 2 cut(s) 118, 295
MaeI CTAG 3 cut(s) 132, 446, 583
MaeIII GTNAC 3 cut(s) 98, 111, 152
MalI GATC 3 cut(s) 66, 184, 195
MboI GATC 3 cut(s) 64, 182, 193
MboII GAAGA 3 cut(s) 205, 411, 492
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 47
MluCI AATT 4 cut(s) 123, 267, 341, 549
MlyI GAGTC 2 cut(s) 396, 538
MnlI CCTC 2 cut(s) 168, 548
Mph1103I ATGCAT 1 cut(s) 319
MroXI GAANNNNTTC 1 cut(s) 56
MseI TTAA 4 cut(s) 12, 87, 189, 353
MslI CAYNNNNRTG 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 233
MspI CCGG 1 cut(s) 371
MspR9I CCNGG 2 cut(s) 207, 372
MvaI CCWGG 1 cut(s) 207
MwoI GCNNNNNNNGC 1 cut(s) 239
NciI CCSGG 1 cut(s) 372
NdeII GATC 3 cut(s) 64, 182, 193
NlaIII CATG 1 cut(s) 317
NlaIV GGNNCC 2 cut(s) 204, 559
NmuCI GTSAC 2 cut(s) 98, 152
NsbI TGCGCA 1 cut(s) 486
NsiI ATGCAT 1 cut(s) 319
PaqCI CACCTGC 1 cut(s) 344
PdmI GAANNNNTTC 1 cut(s) 56
PflMI CCANNNNNTGG 1 cut(s) 319
PkrI GCNGC 3 cut(s) 171, 241, 244
PleI GAGTC 2 cut(s) 396, 538
PpsI GAGTC 2 cut(s) 396, 538
PshBI ATTAAT 2 cut(s) 12, 87
PsiI TTATAA 1 cut(s) 519
Psp6I CCWGG 1 cut(s) 205
PspFI CCCAGC 1 cut(s) 218
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 2 cut(s) 204, 559
PsuI RGATCY 1 cut(s) 193
RsaI GTAC 1 cut(s) 475
RsaNI GTAC 1 cut(s) 474
RseI CAYNNNNRTG 1 cut(s) 393
SaqAI TTAA 4 cut(s) 12, 87, 189, 353
SatI GCNGC 3 cut(s) 170, 240, 243
Sau3AI GATC 3 cut(s) 64, 182, 193
SchI GAGTC 2 cut(s) 396, 538
ScrFI CCNGG 2 cut(s) 207, 372
SduI GDGCHC 1 cut(s) 47
SetI ASST 2 cut(s) 338, 559
SfaNI GCATC 2 cut(s) 118, 295
SmiMI CAYNNNNRTG 1 cut(s) 393
Sse9I AATT 4 cut(s) 123, 267, 341, 549
SsiI CCGC 2 cut(s) 233, 243
SspMI CTAG 3 cut(s) 132, 446, 583
StyD4I CCNGG 2 cut(s) 205, 370
StyI CCWWGG 2 cut(s) 94, 568
TaaI ACNGT 2 cut(s) 49, 358
TaqI TCGA 1 cut(s) 391
TasI AATT 4 cut(s) 123, 267, 341, 549
TauI GCSGC 1 cut(s) 245
Tru1I TTAA 4 cut(s) 12, 87, 189, 353
Tru9I TTAA 4 cut(s) 12, 87, 189, 353
TscAI CASTG 2 cut(s) 52, 434
TseFI GTSAC 2 cut(s) 98, 152
TseI GCWGC 2 cut(s) 169, 239
Tsp45I GTSAC 2 cut(s) 98, 152
TspDTI ATGAA 2 cut(s) 377, 492
TspRI CASTG 2 cut(s) 52, 434
Van91I CCANNNNNTGG 1 cut(s) 319
VspI ATTAAT 2 cut(s) 12, 87
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XmnI GAANNNNTTC 1 cut(s) 56
XspI CTAG 3 cut(s) 132, 446, 583
Zsp2I ATGCAT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.