Rroxscaffold_7G00188210

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010635
Physical Location & Seq
Reverse (-)
27753098 .. 27757224
4127 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00188210.1

Sequence Viewer

Length: 741 bp
ATGAGTACCATAGGAGACATCCAGCTGCACGCACTAGTTGTTGATGCCAATGAGGAACATGAAGCACTGCTCAAACAGGAGCCTCAAGTGGAGAGAATGTTGTGGAGCACTGTGAAGTCCTTCCTTGATCCCAAGACAACTGAAAAGATTAATGTCAGTATACCTTTTGAGCTAAAAGTTATAGTTCTTGTCAGGACCATATTATTAACTAGTGGAAGTTTATTCCTCCAGGTCCTTGGTGACAAGAGCAAGTTACTTCAAATAATTGATGCTAGATGGCTTTTCTTGGCGTGTCACCCTCGCTTGTGGCTGCGAGTTGAACGATCCATTAAAGATCCATTGGAGCCTGGCGTCTTGCCTAGCATTGAGACAGCGGTTGCTGCCGCAAGTTACTTTGTTCAGATTGGTGGAGGTGAGCTGCCGGATGAAACAACCCTCATTTGTTCTCGAGGTGCAGACAGGTTTGCAAATGCATTGGAGTTCCTGTCCACCTGCAAATTAGCAAACTTAACTGTAAAGGCAGAGCCGGGTTGTTGCTTGCTTCATAGAAGTGGAAGACTCACAATAGATGGGTGCGTGCTTCAGTGCCAGACAAATCCCCTAGACTTCCTTTCAGTCCCCATCGTGAGTACGGCAAGTGCGCAAACTCTGTTGTCTTCATCGGTGAAGTGTTATAAGGATGGTGTTTCTGTTTCTCTGACTCAAATTGGAGGTGCCAACTGCCAAGGCTGTTTCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

246

Amino Acids

26.78

Weight (kDa)

5.83

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 675
AarI CACCTGC 1 cut(s) 500
Acc16I TGCGCA 1 cut(s) 642
Acc36I ACCTGC 1 cut(s) 500
AccB1I GGYRCC 1 cut(s) 713
AccI GTMKAC 1 cut(s) 160
AciI CCGC 2 cut(s) 374, 384
AclWI GGATC 3 cut(s) 122, 318, 329
AcuI CTGAAG 1 cut(s) 566
AcyI GRCGYC 1 cut(s) 351
AfaI GTAC 2 cut(s) 7, 631
AgsI TTSAA 3 cut(s) 260, 320, 736
AhlI ACTAGT 2 cut(s) 34, 209
AjnI CCWGG 2 cut(s) 228, 346
AluBI AGCT 3 cut(s) 25, 172, 418
AluI AGCT 3 cut(s) 25, 172, 418
Alw21I GWGCWC 1 cut(s) 110
Alw26I GTCTC 2 cut(s) 9, 362
AlwI GGATC 3 cut(s) 122, 318, 329
Ama87I CYCGRG 1 cut(s) 447
ApeKI GCWGC 4 cut(s) 25, 310, 380, 418
AseI ATTAAT 1 cut(s) 150
Asp700I GAANNNNTTC 1 cut(s) 119
AspLEI GCGC 1 cut(s) 643
AspS9I GGNCC 2 cut(s) 195, 232
AsuC2I CCSGG 1 cut(s) 528
AsuHPI GGTGA 4 cut(s) 251, 287, 425, 676
AvaI CYCGRG 1 cut(s) 447
AvaII GGWCC 2 cut(s) 195, 232
BanI GGYRCC 1 cut(s) 713
BbsI GAAGAC 2 cut(s) 562, 648
Bbv12I GWGCWC 1 cut(s) 110
BbvI GCAGC 4 cut(s) 12, 297, 367, 405
BccI CCATC 4 cut(s) 270, 563, 629, 674
BceAI ACGGC 1 cut(s) 648
BciT130I CCWGG 2 cut(s) 230, 348
BcnI CCSGG 1 cut(s) 528
BcoDI GTCTC 2 cut(s) 9, 362
BcuI ACTAGT 2 cut(s) 34, 209
BfaI CTAG 6 cut(s) 35, 210, 273, 360, 602, 739
BfuAI ACCTGC 1 cut(s) 500
BisI GCNGC 5 cut(s) 26, 311, 381, 384, 419
BlsI GCNGC 5 cut(s) 27, 312, 382, 385, 420
Bme1390I CCNGG 3 cut(s) 230, 348, 528
Bme18I GGWCC 2 cut(s) 195, 232
BmeT110I CYCGRG 1 cut(s) 447
BmgT120I GGNCC 2 cut(s) 195, 232
BmiI GGNNCC 3 cut(s) 81, 345, 715
BmrFI CCNGG 3 cut(s) 230, 348, 528
BmsI GCATC 2 cut(s) 34, 259
BpiI GAAGAC 2 cut(s) 562, 648
BpmI CTGGAG 1 cut(s) 212
BpuEI CTTGAG 1 cut(s) 69
BpuMI CCSGG 1 cut(s) 528
BsaHI GRCGYC 1 cut(s) 351
BsaJI CCNNGG 2 cut(s) 235, 724
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 470, 500
BseBI CCWGG 2 cut(s) 230, 348
BseDI CCNNGG 2 cut(s) 235, 724
BseGI GGATG 3 cut(s) 18, 430, 685
BseXI GCAGC 4 cut(s) 12, 297, 367, 405
BsgI GTGCAG 2 cut(s) 11, 474
BshNI GGYRCC 1 cut(s) 713
BsiHKAI GWGCWC 1 cut(s) 110
BsiHKCI CYCGRG 1 cut(s) 447
BsiSI CCGG 2 cut(s) 422, 527
BslFI GGGAC 1 cut(s) 602
BsmAI GTCTC 2 cut(s) 9, 362
BsmFI GGGAC 1 cut(s) 602
BsoBI CYCGRG 1 cut(s) 447
Bsp1286I GDGCHC 1 cut(s) 110
Bsp143I GATC 3 cut(s) 127, 323, 334
BspACI CCGC 2 cut(s) 374, 384
BspLI GGNNCC 3 cut(s) 81, 345, 715
BspMI ACCTGC 1 cut(s) 500
BspPI GGATC 3 cut(s) 122, 318, 329
BspT107I GGYRCC 1 cut(s) 713
BssECI CCNNGG 2 cut(s) 235, 724
BssMI GATC 3 cut(s) 127, 323, 334
BssNAI GTATAC 1 cut(s) 161
BssNI GRCGYC 1 cut(s) 351
BssT1I CCWWGG 2 cut(s) 235, 724
Bst1107I GTATAC 1 cut(s) 161
Bst2UI CCWGG 2 cut(s) 230, 348
Bst4CI ACNGT 2 cut(s) 112, 514
BstACI GRCGYC 1 cut(s) 351
BstC8I GCNNGC 3 cut(s) 30, 539, 578
BstF5I GGATG 3 cut(s) 18, 430, 685
BstHHI GCGC 1 cut(s) 643
BstKTI GATC 3 cut(s) 130, 326, 337
BstMAI GTCTC 2 cut(s) 9, 362
BstMBI GATC 3 cut(s) 127, 323, 334
BstMWI GCNNNNNNNGC 1 cut(s) 380
BstNI CCWGG 2 cut(s) 230, 348
BstSCI CCNGG 3 cut(s) 228, 346, 526
BstV1I GCAGC 4 cut(s) 12, 297, 367, 405
BstV2I GAAGAC 2 cut(s) 562, 648
BstX2I RGATCY 1 cut(s) 334
BstXI CCANNNNNNTGG 1 cut(s) 236
BstYI RGATCY 1 cut(s) 334
BstZ17I GTATAC 1 cut(s) 161
BtsCI GGATG 3 cut(s) 18, 430, 685
BtsI GCAGTG 1 cut(s) 65
BtsIMutI CAGTG 3 cut(s) 65, 108, 590
BveI ACCTGC 1 cut(s) 500
Cac8I GCNNGC 3 cut(s) 30, 539, 578
CfoI GCGC 1 cut(s) 643
Cfr13I GGNCC 2 cut(s) 195, 232
CseI GACGC 1 cut(s) 340
Csp6I GTAC 2 cut(s) 6, 630
CviAII CATG 1 cut(s) 59
CviJI RGCY 9 cut(s) 25, 82, 172, 280, 310, 346, 418, 526, 729
CviKI_1 RGCY 9 cut(s) 25, 82, 172, 280, 310, 346, 418, 526, 729
CviQI GTAC 2 cut(s) 6, 630
DpnI GATC 3 cut(s) 129, 325, 336
DpnII GATC 3 cut(s) 127, 323, 334
Eco130I CCWWGG 2 cut(s) 235, 724
Eco47I GGWCC 2 cut(s) 195, 232
Eco57I CTGAAG 1 cut(s) 566
Eco88I CYCGRG 1 cut(s) 447
EcoO109I RGGNCCY 1 cut(s) 232
EcoRII CCWGG 2 cut(s) 228, 346
EcoT14I CCWWGG 2 cut(s) 235, 724
EcoT22I ATGCAT 1 cut(s) 475
ErhI CCWWGG 2 cut(s) 235, 724
FaeI CATG 1 cut(s) 62
FaiI YATR 7 cut(s) 11, 60, 161, 182, 200, 546, 675
FaqI GGGAC 1 cut(s) 602
FatI CATG 1 cut(s) 58
FblI GTMKAC 1 cut(s) 160
Fnu4HI GCNGC 5 cut(s) 26, 311, 381, 384, 419
FokI GGATG 3 cut(s) 5, 437, 692
Fsp4HI GCNGC 5 cut(s) 26, 311, 381, 384, 419
FspBI CTAG 6 cut(s) 35, 210, 273, 360, 602, 739
FspI TGCGCA 1 cut(s) 642
GlaI GCGC 1 cut(s) 642
GluI GCNGC 5 cut(s) 26, 311, 381, 384, 419
GsuI CTGGAG 1 cut(s) 212
HapII CCGG 2 cut(s) 422, 527
HgaI GACGC 1 cut(s) 340
HhaI GCGC 1 cut(s) 643
Hin1I GRCGYC 1 cut(s) 351
Hin1II CATG 1 cut(s) 62
Hin6I GCGC 1 cut(s) 641
HinP1I GCGC 1 cut(s) 641
HinfI GANTC 2 cut(s) 558, 700
HpaII CCGG 2 cut(s) 422, 527
HphI GGTGA 4 cut(s) 251, 287, 425, 676
Hpy166II GTNNAC 2 cut(s) 161, 489
Hpy188I TCNGA 2 cut(s) 402, 699
Hpy188III TCNNGA 3 cut(s) 193, 447, 625
Hpy8I GTNNAC 2 cut(s) 161, 489
HpyAV CCTTC 1 cut(s) 130
HpyCH4III ACNGT 2 cut(s) 112, 514
HpyCH4V TGCA 5 cut(s) 28, 455, 467, 473, 495
HpyF10VI GCNNNNNNNGC 1 cut(s) 380
Hsp92I GRCGYC 1 cut(s) 351
Hsp92II CATG 1 cut(s) 62
HspAI GCGC 1 cut(s) 641
Kzo9I GATC 3 cut(s) 127, 323, 334
LmnI GCTCC 3 cut(s) 79, 105, 343
Lsp1109I GCAGC 4 cut(s) 12, 297, 367, 405
LweI GCATC 2 cut(s) 34, 259
MaeI CTAG 6 cut(s) 35, 210, 273, 360, 602, 739
MaeIII GTNAC 4 cut(s) 239, 252, 293, 389
MalI GATC 3 cut(s) 129, 325, 336
MboI GATC 3 cut(s) 127, 323, 334
MboII GAAGA 2 cut(s) 567, 648
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 1 cut(s) 110
MluCI AATT 3 cut(s) 264, 497, 705
MlyI GAGTC 2 cut(s) 552, 694
MnlI CCTC 8 cut(s) 46, 93, 236, 309, 404, 443, 446, 704
Mph1103I ATGCAT 1 cut(s) 475
MroXI GAANNNNTTC 1 cut(s) 119
MseI TTAA 4 cut(s) 150, 206, 330, 509
MslI CAYNNNNRTG 1 cut(s) 549
MspA1I CMGCKG 2 cut(s) 25, 374
MspI CCGG 2 cut(s) 422, 527
MspR9I CCNGG 3 cut(s) 230, 348, 528
MvaI CCWGG 2 cut(s) 230, 348
MwoI GCNNNNNNNGC 1 cut(s) 380
NciI CCSGG 1 cut(s) 528
NdeII GATC 3 cut(s) 127, 323, 334
NlaIII CATG 1 cut(s) 62
NlaIV GGNNCC 3 cut(s) 81, 345, 715
NmuCI GTSAC 2 cut(s) 239, 293
NsbI TGCGCA 1 cut(s) 642
NsiI ATGCAT 1 cut(s) 475
PaeR7I CTCGAG 1 cut(s) 447
PaqCI CACCTGC 1 cut(s) 500
PdmI GAANNNNTTC 1 cut(s) 119
PkrI GCNGC 5 cut(s) 27, 312, 382, 385, 420
PleI GAGTC 2 cut(s) 552, 694
PpsI GAGTC 2 cut(s) 552, 694
PpuMI RGGWCCY 1 cut(s) 232
PshBI ATTAAT 1 cut(s) 150
PsiI TTATAA 1 cut(s) 675
Psp5II RGGWCCY 1 cut(s) 232
Psp6I CCWGG 2 cut(s) 228, 346
PspGI CCWGG 2 cut(s) 228, 346
PspN4I GGNNCC 3 cut(s) 81, 345, 715
PspPI GGNCC 2 cut(s) 195, 232
PspPPI RGGWCCY 1 cut(s) 232
PsuI RGATCY 1 cut(s) 334
PvuII CAGCTG 1 cut(s) 25
RsaI GTAC 2 cut(s) 7, 631
RsaNI GTAC 2 cut(s) 6, 630
RseI CAYNNNNRTG 1 cut(s) 549
SaqAI TTAA 4 cut(s) 150, 206, 330, 509
SatI GCNGC 5 cut(s) 26, 311, 381, 384, 419
Sau3AI GATC 3 cut(s) 127, 323, 334
Sau96I GGNCC 2 cut(s) 195, 232
SchI GAGTC 2 cut(s) 552, 694
ScrFI CCNGG 3 cut(s) 230, 348, 528
SduI GDGCHC 1 cut(s) 110
SfaNI GCATC 2 cut(s) 34, 259
Sfr274I CTCGAG 1 cut(s) 447
SinI GGWCC 2 cut(s) 195, 232
SlaI CTCGAG 1 cut(s) 447
SmiMI CAYNNNNRTG 1 cut(s) 549
SmlI CTYRAG 2 cut(s) 84, 447
SmoI CTYRAG 2 cut(s) 84, 447
SpeI ACTAGT 2 cut(s) 34, 209
Sse9I AATT 3 cut(s) 264, 497, 705
SsiI CCGC 2 cut(s) 374, 384
SspMI CTAG 6 cut(s) 35, 210, 273, 360, 602, 739
StyD4I CCNGG 3 cut(s) 228, 346, 526
StyI CCWWGG 2 cut(s) 235, 724
TaaI ACNGT 2 cut(s) 112, 514
TaqI TCGA 1 cut(s) 448
TasI AATT 3 cut(s) 264, 497, 705
TauI GCSGC 1 cut(s) 386
Tru1I TTAA 4 cut(s) 150, 206, 330, 509
Tru9I TTAA 4 cut(s) 150, 206, 330, 509
TscAI CASTG 3 cut(s) 72, 115, 590
TseFI GTSAC 2 cut(s) 239, 293
TseI GCWGC 4 cut(s) 25, 310, 380, 418
Tsp45I GTSAC 2 cut(s) 239, 293
TspDTI ATGAA 4 cut(s) 75, 441, 533, 648
TspRI CASTG 3 cut(s) 72, 115, 590
VpaK11BI GGWCC 2 cut(s) 195, 232
VspI ATTAAT 1 cut(s) 150
XhoI CTCGAG 1 cut(s) 447
XmiI GTMKAC 1 cut(s) 160
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 6 cut(s) 35, 210, 273, 360, 602, 739
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.