Rroxscaffold_6G00400510

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0010635
Physical Location & Seq
Forward (+)
22609257 .. 22613389
4133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00400510.1

Sequence Viewer

Length: 756 bp
ATGATTGATTCGCCTTCAGTGAGATTGGAACTTATCACTTGCCTTCAGACGATTGATAGCAACAACTATCCTGAGACTGTGAATTGTATGTTTATCATTAATGCTGGTTCTGGTTTTAGAATGTTGTGGAGCACTGTGAAGTCCTTCCTTGATCCCAAGACAACTGAAAAGATTAATGTCAGTATACCTTTTGAGCTAAAAGTTATAGTTCTTGTCAGGACCATATTATTAACTAGTGGAAGTTTATTCCTCCAGGTCCTTGGTGACAAGAGCAAGTTACTTCAAATAATTGATGCTAGATGGCTTTTCTTGGCGTGTCACCCTCGCTTGTGGCTGCGAGTTGAACGATCCATTAAAGATCCATTGGAGCCTGGCGTCTTGCCTAGCATTGAGACAGCGGTTGCTGCCGCAAGTTACTTTGTTCAGATTGGTGGAGGTGAGCTGCCGGATGAAACAACCCTCATTTGTTCTCGAGGTGCAGACAGTGCATTGGAGTTCCTGTCCACCTGCAAATTAGCAAACTTAACTGTAAAGGCAGAGCCGGGTTGTTGCTTGCTTCATAGAAGTGGAAGACTCACAATAGATGGGTGCGTGCTTCAGTGCCAGACAAATCCCCTAGACTTCCTTTCAGTCCCCATCGTGAGTACGGCAAGTGCGCAAACTCTGTTGTCTTCATCGGTGAAGTGTTATAAGGATGGTGTTTCTGTTTCTCTGACTCAAATTGGAGGTGCCAACTGCCAAGGCTGTTTCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

251

Amino Acids

27.23

Weight (kDa)

6.13

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 17 - 60 6.6e-10 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 690
AarI CACCTGC 1 cut(s) 515
Acc16I TGCGCA 1 cut(s) 657
Acc36I ACCTGC 1 cut(s) 515
AccB1I GGYRCC 1 cut(s) 728
AccI GTMKAC 1 cut(s) 184
AciI CCGC 2 cut(s) 398, 408
AclWI GGATC 3 cut(s) 146, 342, 353
AcuI CTGAAG 2 cut(s) 29, 581
AcyI GRCGYC 1 cut(s) 375
AfaI GTAC 1 cut(s) 646
AgsI TTSAA 3 cut(s) 284, 344, 751
AhlI ACTAGT 1 cut(s) 233
AjnI CCWGG 2 cut(s) 252, 370
AluBI AGCT 2 cut(s) 196, 442
AluI AGCT 2 cut(s) 196, 442
Alw21I GWGCWC 1 cut(s) 134
Alw26I GTCTC 2 cut(s) 68, 386
AlwI GGATC 3 cut(s) 146, 342, 353
Ama87I CYCGRG 1 cut(s) 471
ApeKI GCWGC 3 cut(s) 334, 404, 442
AseI ATTAAT 2 cut(s) 99, 174
Asp700I GAANNNNTTC 1 cut(s) 143
AspLEI GCGC 1 cut(s) 658
AspS9I GGNCC 2 cut(s) 219, 256
AsuC2I CCSGG 1 cut(s) 543
AsuHPI GGTGA 4 cut(s) 275, 311, 449, 691
AvaI CYCGRG 1 cut(s) 471
AvaII GGWCC 2 cut(s) 219, 256
BanI GGYRCC 1 cut(s) 728
BbsI GAAGAC 2 cut(s) 577, 663
Bbv12I GWGCWC 1 cut(s) 134
BbvI GCAGC 3 cut(s) 321, 391, 429
BccI CCATC 4 cut(s) 294, 578, 644, 689
BceAI ACGGC 1 cut(s) 663
BciT130I CCWGG 2 cut(s) 254, 372
BcnI CCSGG 1 cut(s) 543
BcoDI GTCTC 2 cut(s) 68, 386
BcuI ACTAGT 1 cut(s) 233
BfaI CTAG 5 cut(s) 234, 297, 384, 617, 754
BfuAI ACCTGC 1 cut(s) 515
BisI GCNGC 4 cut(s) 335, 405, 408, 443
BlsI GCNGC 4 cut(s) 336, 406, 409, 444
Bme1390I CCNGG 3 cut(s) 254, 372, 543
Bme18I GGWCC 2 cut(s) 219, 256
BmeT110I CYCGRG 1 cut(s) 471
BmgT120I GGNCC 2 cut(s) 219, 256
BmiI GGNNCC 2 cut(s) 369, 730
BmrFI CCNGG 3 cut(s) 254, 372, 543
BmsI GCATC 1 cut(s) 283
BpiI GAAGAC 2 cut(s) 577, 663
BpmI CTGGAG 1 cut(s) 236
BpuMI CCSGG 1 cut(s) 543
BsaHI GRCGYC 1 cut(s) 375
BsaJI CCNNGG 2 cut(s) 259, 739
BsaXI ACNNNNNCTCC 2 cut(s) 485, 515
BseBI CCWGG 2 cut(s) 254, 372
BseDI CCNNGG 2 cut(s) 259, 739
BseGI GGATG 2 cut(s) 454, 700
BseMII CTCAG 1 cut(s) 63
BseXI GCAGC 3 cut(s) 321, 391, 429
BsgI GTGCAG 1 cut(s) 498
BshNI GGYRCC 1 cut(s) 728
BsiHKAI GWGCWC 1 cut(s) 134
BsiHKCI CYCGRG 1 cut(s) 471
BsiSI CCGG 2 cut(s) 446, 542
BslFI GGGAC 1 cut(s) 617
BsmAI GTCTC 2 cut(s) 68, 386
BsmFI GGGAC 1 cut(s) 617
BsoBI CYCGRG 1 cut(s) 471
Bsp1286I GDGCHC 1 cut(s) 134
Bsp143I GATC 3 cut(s) 151, 347, 358
BspACI CCGC 2 cut(s) 398, 408
BspCNI CTCAG 1 cut(s) 64
BspLI GGNNCC 2 cut(s) 369, 730
BspMI ACCTGC 1 cut(s) 515
BspPI GGATC 3 cut(s) 146, 342, 353
BspT107I GGYRCC 1 cut(s) 728
BssECI CCNNGG 2 cut(s) 259, 739
BssMI GATC 3 cut(s) 151, 347, 358
BssNAI GTATAC 1 cut(s) 185
BssNI GRCGYC 1 cut(s) 375
BssT1I CCWWGG 2 cut(s) 259, 739
Bst1107I GTATAC 1 cut(s) 185
Bst2UI CCWGG 2 cut(s) 254, 372
Bst4CI ACNGT 4 cut(s) 79, 136, 485, 529
BstACI GRCGYC 1 cut(s) 375
BstAPI GCANNNNNTGC 1 cut(s) 485
BstC8I GCNNGC 2 cut(s) 554, 593
BstDEI CTNAG 1 cut(s) 72
BstF5I GGATG 2 cut(s) 454, 700
BstHHI GCGC 1 cut(s) 658
BstKTI GATC 3 cut(s) 154, 350, 361
BstMAI GTCTC 2 cut(s) 68, 386
BstMBI GATC 3 cut(s) 151, 347, 358
BstMWI GCNNNNNNNGC 2 cut(s) 404, 485
BstNI CCWGG 2 cut(s) 254, 372
BstSCI CCNGG 3 cut(s) 252, 370, 541
BstV1I GCAGC 3 cut(s) 321, 391, 429
BstV2I GAAGAC 2 cut(s) 577, 663
BstX2I RGATCY 1 cut(s) 358
BstXI CCANNNNNNTGG 1 cut(s) 260
BstYI RGATCY 1 cut(s) 358
BstZ17I GTATAC 1 cut(s) 185
BtsCI GGATG 2 cut(s) 454, 700
BtsIMutI CAGTG 4 cut(s) 24, 132, 490, 605
BveI ACCTGC 1 cut(s) 515
Cac8I GCNNGC 2 cut(s) 554, 593
CfoI GCGC 1 cut(s) 658
Cfr13I GGNCC 2 cut(s) 219, 256
CseI GACGC 1 cut(s) 364
Csp6I GTAC 1 cut(s) 645
CviJI RGCY 7 cut(s) 196, 304, 334, 370, 442, 541, 744
CviKI_1 RGCY 7 cut(s) 196, 304, 334, 370, 442, 541, 744
CviQI GTAC 1 cut(s) 645
DdeI CTNAG 1 cut(s) 72
DpnI GATC 3 cut(s) 153, 349, 360
DpnII GATC 3 cut(s) 151, 347, 358
Eco130I CCWWGG 2 cut(s) 259, 739
Eco47I GGWCC 2 cut(s) 219, 256
Eco57I CTGAAG 2 cut(s) 29, 581
Eco88I CYCGRG 1 cut(s) 471
EcoO109I RGGNCCY 1 cut(s) 256
EcoRII CCWGG 2 cut(s) 252, 370
EcoT14I CCWWGG 2 cut(s) 259, 739
ErhI CCWWGG 2 cut(s) 259, 739
FaiI YATR 6 cut(s) 89, 185, 206, 224, 561, 690
FaqI GGGAC 1 cut(s) 617
FblI GTMKAC 1 cut(s) 184
Fnu4HI GCNGC 4 cut(s) 335, 405, 408, 443
FokI GGATG 2 cut(s) 461, 707
Fsp4HI GCNGC 4 cut(s) 335, 405, 408, 443
FspBI CTAG 5 cut(s) 234, 297, 384, 617, 754
FspI TGCGCA 1 cut(s) 657
GlaI GCGC 1 cut(s) 657
GluI GCNGC 4 cut(s) 335, 405, 408, 443
GsuI CTGGAG 1 cut(s) 236
HapII CCGG 2 cut(s) 446, 542
HgaI GACGC 1 cut(s) 364
HhaI GCGC 1 cut(s) 658
Hin1I GRCGYC 1 cut(s) 375
Hin6I GCGC 1 cut(s) 656
HinP1I GCGC 1 cut(s) 656
HinfI GANTC 3 cut(s) 8, 573, 715
HpaII CCGG 2 cut(s) 446, 542
HphI GGTGA 4 cut(s) 275, 311, 449, 691
Hpy166II GTNNAC 2 cut(s) 185, 504
Hpy188I TCNGA 3 cut(s) 48, 426, 714
Hpy188III TCNNGA 4 cut(s) 71, 217, 471, 640
Hpy8I GTNNAC 2 cut(s) 185, 504
HpyAV CCTTC 3 cut(s) 24, 53, 154
HpyCH4III ACNGT 4 cut(s) 79, 136, 485, 529
HpyCH4V TGCA 3 cut(s) 479, 488, 510
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 485
HpyF3I CTNAG 1 cut(s) 72
Hsp92I GRCGYC 1 cut(s) 375
HspAI GCGC 1 cut(s) 656
Kzo9I GATC 3 cut(s) 151, 347, 358
LmnI GCTCC 2 cut(s) 129, 367
Lsp1109I GCAGC 3 cut(s) 321, 391, 429
LweI GCATC 1 cut(s) 283
MaeI CTAG 5 cut(s) 234, 297, 384, 617, 754
MaeIII GTNAC 4 cut(s) 263, 276, 317, 413
MalI GATC 3 cut(s) 153, 349, 360
MboI GATC 3 cut(s) 151, 347, 358
MboII GAAGA 2 cut(s) 582, 663
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 1 cut(s) 134
MluCI AATT 4 cut(s) 82, 288, 512, 720
MlyI GAGTC 2 cut(s) 567, 709
MnlI CCTC 6 cut(s) 260, 333, 428, 467, 470, 719
MroXI GAANNNNTTC 1 cut(s) 143
MseI TTAA 5 cut(s) 99, 174, 230, 354, 524
MslI CAYNNNNRTG 1 cut(s) 564
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 2 cut(s) 446, 542
MspR9I CCNGG 3 cut(s) 254, 372, 543
MvaI CCWGG 2 cut(s) 254, 372
MwoI GCNNNNNNNGC 2 cut(s) 404, 485
NciI CCSGG 1 cut(s) 543
NdeII GATC 3 cut(s) 151, 347, 358
NlaIV GGNNCC 2 cut(s) 369, 730
NmuCI GTSAC 2 cut(s) 263, 317
NsbI TGCGCA 1 cut(s) 657
PaeR7I CTCGAG 1 cut(s) 471
PaqCI CACCTGC 1 cut(s) 515
PdmI GAANNNNTTC 1 cut(s) 143
PfeI GAWTC 1 cut(s) 8
PkrI GCNGC 4 cut(s) 336, 406, 409, 444
PleI GAGTC 2 cut(s) 567, 709
PpsI GAGTC 2 cut(s) 567, 709
PpuMI RGGWCCY 1 cut(s) 256
PshBI ATTAAT 2 cut(s) 99, 174
PsiI TTATAA 1 cut(s) 690
Psp5II RGGWCCY 1 cut(s) 256
Psp6I CCWGG 2 cut(s) 252, 370
PspGI CCWGG 2 cut(s) 252, 370
PspN4I GGNNCC 2 cut(s) 369, 730
PspPI GGNCC 2 cut(s) 219, 256
PspPPI RGGWCCY 1 cut(s) 256
PsuI RGATCY 1 cut(s) 358
RsaI GTAC 1 cut(s) 646
RsaNI GTAC 1 cut(s) 645
RseI CAYNNNNRTG 1 cut(s) 564
SaqAI TTAA 5 cut(s) 99, 174, 230, 354, 524
SatI GCNGC 4 cut(s) 335, 405, 408, 443
Sau3AI GATC 3 cut(s) 151, 347, 358
Sau96I GGNCC 2 cut(s) 219, 256
SchI GAGTC 2 cut(s) 567, 709
ScrFI CCNGG 3 cut(s) 254, 372, 543
SduI GDGCHC 1 cut(s) 134
SetI ASST 8 cut(s) 190, 198, 258, 439, 444, 478, 509, 730
SfaNI GCATC 1 cut(s) 283
Sfr274I CTCGAG 1 cut(s) 471
SinI GGWCC 2 cut(s) 219, 256
SlaI CTCGAG 1 cut(s) 471
SmiMI CAYNNNNRTG 1 cut(s) 564
SmlI CTYRAG 1 cut(s) 471
SmoI CTYRAG 1 cut(s) 471
SpeI ACTAGT 1 cut(s) 233
Sse9I AATT 4 cut(s) 82, 288, 512, 720
SsiI CCGC 2 cut(s) 398, 408
SspMI CTAG 5 cut(s) 234, 297, 384, 617, 754
StyD4I CCNGG 3 cut(s) 252, 370, 541
StyI CCWWGG 2 cut(s) 259, 739
TaaI ACNGT 4 cut(s) 79, 136, 485, 529
TaqI TCGA 1 cut(s) 472
TasI AATT 4 cut(s) 82, 288, 512, 720
TauI GCSGC 1 cut(s) 410
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 5 cut(s) 99, 174, 230, 354, 524
Tru9I TTAA 5 cut(s) 99, 174, 230, 354, 524
TscAI CASTG 4 cut(s) 24, 139, 490, 605
TseFI GTSAC 2 cut(s) 263, 317
TseI GCWGC 3 cut(s) 334, 404, 442
Tsp45I GTSAC 2 cut(s) 263, 317
TspDTI ATGAA 3 cut(s) 465, 548, 663
TspRI CASTG 4 cut(s) 24, 139, 490, 605
VpaK11BI GGWCC 2 cut(s) 219, 256
VspI ATTAAT 2 cut(s) 99, 174
XhoI CTCGAG 1 cut(s) 471
XmiI GTMKAC 1 cut(s) 184
XmnI GAANNNNTTC 1 cut(s) 143
XspI CTAG 5 cut(s) 234, 297, 384, 617, 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.