Rroxscaffold_4G00308150

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010635
Physical Location & Seq
Forward (+)
29689773 .. 29693815
4043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00308150.1

Sequence Viewer

Length: 846 bp
ATGATTGATTCGCCTTCAGTGAGATTGGAACTTATCACTTGCCTTCAGACGATTGATAGCAACAACTATCCTGAGACTGTGAATTGTATGTTTATCATTAATGCTGGTTCTGGTTTTAGAATGTTGTGGAGCACTGTGAAGTCCTTCCTTGATCCCAAGACAACTGAAAAGATTAATGTCAGTATACCTTTTGAGCTAAAAGTTATAGTTCTTGTCAGGACCATATTATTAACTAGTGGAAGTTTATTCCTCCAGGTCCTTGGTGACAAGAGCAAGTTACTTCAAATAATTGATGCTAGCCCACACATGTATGACAAAGTTGTTCTATTTCTGTCTCATAATTCTATTGAGTTTGCGGATCGCTATAACACCACCCTAGTTTGCCACAGATGGCTTTTCTTGGCGTGTCACCCTCGCTTGTGGCTGCGAGTTGAACGATCCATTAAAGATCCATTGGAGCCTGGCGTCTTGCCTAGCATTGAGACAGCGGTTGCTGCCGCAAGTTACTTTGTTCAGATTGGTGGAGGTGAGCTGCCGGATGAAACAACCCTCATTTGTTCTCGAGGTGCAGACAGTGCATTGGAGTTCCTGTCCACCTGCAAATTAGCAAACTTAACTGTAAAGGCAGAGCTGGGTTGTTGCTTGCTTCATAGAAGTGGAAGACTCACAATAGATGGGTGCGTGCTTCAGTGCCAGACAAATCCCCTAGACTTCCTTTCAGTCCCCATCGTGAGTACGGCAAGTGCGCAAACTCTGTTGTCTTCATCGGTGAAGTGTTATAAGGATGGTGTTTCTGTTTCTCTGACTCAAATTGGAGGTGCCAACTGCCAAGGCTGTTTCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

281

Amino Acids

30.77

Weight (kDa)

6.21

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 17 - 60 7.8e-10 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 780
AarI CACCTGC 1 cut(s) 605
Acc16I TGCGCA 1 cut(s) 747
Acc36I ACCTGC 1 cut(s) 605
AccB1I GGYRCC 1 cut(s) 818
AccI GTMKAC 1 cut(s) 184
AciI CCGC 3 cut(s) 356, 488, 498
AclWI GGATC 4 cut(s) 146, 366, 432, 443
AcuI CTGAAG 2 cut(s) 29, 671
AcyI GRCGYC 1 cut(s) 465
AfaI GTAC 1 cut(s) 736
AflIII ACRYGT 1 cut(s) 306
AgsI TTSAA 3 cut(s) 284, 434, 841
AhlI ACTAGT 1 cut(s) 233
AjnI CCWGG 2 cut(s) 252, 460
AluBI AGCT 3 cut(s) 196, 532, 631
AluI AGCT 3 cut(s) 196, 532, 631
Alw21I GWGCWC 1 cut(s) 134
Alw26I GTCTC 3 cut(s) 68, 339, 476
AlwI GGATC 4 cut(s) 146, 366, 432, 443
Ama87I CYCGRG 1 cut(s) 561
ApeKI GCWGC 3 cut(s) 424, 494, 532
AseI ATTAAT 2 cut(s) 99, 174
Asp700I GAANNNNTTC 1 cut(s) 143
AspLEI GCGC 1 cut(s) 748
AspS9I GGNCC 2 cut(s) 219, 256
AsuHPI GGTGA 4 cut(s) 275, 401, 539, 781
AsuNHI GCTAGC 1 cut(s) 296
AvaI CYCGRG 1 cut(s) 561
AvaII GGWCC 2 cut(s) 219, 256
BanI GGYRCC 1 cut(s) 818
BbsI GAAGAC 2 cut(s) 667, 753
Bbv12I GWGCWC 1 cut(s) 134
BbvI GCAGC 3 cut(s) 411, 481, 519
BccI CCATC 4 cut(s) 384, 668, 734, 779
BceAI ACGGC 1 cut(s) 753
BciT130I CCWGG 2 cut(s) 254, 462
BcoDI GTCTC 3 cut(s) 68, 339, 476
BcuI ACTAGT 1 cut(s) 233
BfaI CTAG 6 cut(s) 234, 297, 377, 474, 707, 844
BfuAI ACCTGC 1 cut(s) 605
BisI GCNGC 4 cut(s) 425, 495, 498, 533
BlsI GCNGC 4 cut(s) 426, 496, 499, 534
Bme1390I CCNGG 2 cut(s) 254, 462
Bme18I GGWCC 2 cut(s) 219, 256
BmeT110I CYCGRG 1 cut(s) 561
BmgT120I GGNCC 2 cut(s) 219, 256
BmiI GGNNCC 2 cut(s) 459, 820
BmrFI CCNGG 2 cut(s) 254, 462
BmsI GCATC 1 cut(s) 283
BmtI GCTAGC 1 cut(s) 300
BpiI GAAGAC 2 cut(s) 667, 753
BpmI CTGGAG 1 cut(s) 236
BsaHI GRCGYC 1 cut(s) 465
BsaJI CCNNGG 2 cut(s) 259, 829
BsaXI ACNNNNNCTCC 2 cut(s) 575, 605
BseBI CCWGG 2 cut(s) 254, 462
BseDI CCNNGG 2 cut(s) 259, 829
BseGI GGATG 2 cut(s) 544, 790
BseMII CTCAG 1 cut(s) 63
BseXI GCAGC 3 cut(s) 411, 481, 519
BseYI CCCAGC 1 cut(s) 631
BsgI GTGCAG 1 cut(s) 588
BshNI GGYRCC 1 cut(s) 818
BsiHKAI GWGCWC 1 cut(s) 134
BsiHKCI CYCGRG 1 cut(s) 561
BsiSI CCGG 1 cut(s) 536
BslFI GGGAC 1 cut(s) 707
BsmAI GTCTC 3 cut(s) 68, 339, 476
BsmFI GGGAC 1 cut(s) 707
BsoBI CYCGRG 1 cut(s) 561
Bsp1286I GDGCHC 1 cut(s) 134
Bsp143I GATC 4 cut(s) 151, 358, 437, 448
BspACI CCGC 3 cut(s) 356, 488, 498
BspCNI CTCAG 1 cut(s) 64
BspLI GGNNCC 2 cut(s) 459, 820
BspMI ACCTGC 1 cut(s) 605
BspOI GCTAGC 1 cut(s) 300
BspPI GGATC 4 cut(s) 146, 366, 432, 443
BspT107I GGYRCC 1 cut(s) 818
BssECI CCNNGG 2 cut(s) 259, 829
BssMI GATC 4 cut(s) 151, 358, 437, 448
BssNAI GTATAC 1 cut(s) 185
BssNI GRCGYC 1 cut(s) 465
BssT1I CCWWGG 2 cut(s) 259, 829
Bst1107I GTATAC 1 cut(s) 185
Bst2UI CCWGG 2 cut(s) 254, 462
Bst4CI ACNGT 4 cut(s) 79, 136, 575, 619
BstACI GRCGYC 1 cut(s) 465
BstAPI GCANNNNNTGC 1 cut(s) 575
BstC8I GCNNGC 3 cut(s) 298, 644, 683
BstDEI CTNAG 1 cut(s) 72
BstF5I GGATG 2 cut(s) 544, 790
BstHHI GCGC 1 cut(s) 748
BstKTI GATC 4 cut(s) 154, 361, 440, 451
BstMAI GTCTC 3 cut(s) 68, 339, 476
BstMBI GATC 4 cut(s) 151, 358, 437, 448
BstMWI GCNNNNNNNGC 2 cut(s) 494, 575
BstNI CCWGG 2 cut(s) 254, 462
BstNSI RCATGY 1 cut(s) 310
BstSCI CCNGG 2 cut(s) 252, 460
BstV1I GCAGC 3 cut(s) 411, 481, 519
BstV2I GAAGAC 2 cut(s) 667, 753
BstX2I RGATCY 1 cut(s) 448
BstXI CCANNNNNNTGG 1 cut(s) 260
BstYI RGATCY 1 cut(s) 448
BstZ17I GTATAC 1 cut(s) 185
BtsCI GGATG 2 cut(s) 544, 790
BtsIMutI CAGTG 4 cut(s) 24, 132, 580, 695
BveI ACCTGC 1 cut(s) 605
Cac8I GCNNGC 3 cut(s) 298, 644, 683
CfoI GCGC 1 cut(s) 748
Cfr13I GGNCC 2 cut(s) 219, 256
CseI GACGC 1 cut(s) 454
Csp6I GTAC 1 cut(s) 735
CviAII CATG 1 cut(s) 307
CviJI RGCY 8 cut(s) 196, 300, 394, 424, 460, 532, 631, 834
CviKI_1 RGCY 8 cut(s) 196, 300, 394, 424, 460, 532, 631, 834
CviQI GTAC 1 cut(s) 735
DdeI CTNAG 1 cut(s) 72
DpnI GATC 4 cut(s) 153, 360, 439, 450
DpnII GATC 4 cut(s) 151, 358, 437, 448
Eco130I CCWWGG 2 cut(s) 259, 829
Eco47I GGWCC 2 cut(s) 219, 256
Eco57I CTGAAG 2 cut(s) 29, 671
Eco88I CYCGRG 1 cut(s) 561
EcoO109I RGGNCCY 1 cut(s) 256
EcoRII CCWGG 2 cut(s) 252, 460
EcoT14I CCWWGG 2 cut(s) 259, 829
ErhI CCWWGG 2 cut(s) 259, 829
FaeI CATG 1 cut(s) 310
FaqI GGGAC 1 cut(s) 707
FatI CATG 1 cut(s) 306
FblI GTMKAC 1 cut(s) 184
Fnu4HI GCNGC 4 cut(s) 425, 495, 498, 533
FokI GGATG 2 cut(s) 551, 797
Fsp4HI GCNGC 4 cut(s) 425, 495, 498, 533
FspBI CTAG 6 cut(s) 234, 297, 377, 474, 707, 844
FspI TGCGCA 1 cut(s) 747
GlaI GCGC 1 cut(s) 747
GluI GCNGC 4 cut(s) 425, 495, 498, 533
GsaI CCCAGC 1 cut(s) 635
GsuI CTGGAG 1 cut(s) 236
HapII CCGG 1 cut(s) 536
HgaI GACGC 1 cut(s) 454
HhaI GCGC 1 cut(s) 748
Hin1I GRCGYC 1 cut(s) 465
Hin1II CATG 1 cut(s) 310
Hin6I GCGC 1 cut(s) 746
HinP1I GCGC 1 cut(s) 746
HinfI GANTC 3 cut(s) 8, 663, 805
HpaII CCGG 1 cut(s) 536
HphI GGTGA 4 cut(s) 275, 401, 539, 781
Hpy166II GTNNAC 2 cut(s) 185, 594
Hpy188I TCNGA 3 cut(s) 48, 516, 804
Hpy188III TCNNGA 4 cut(s) 71, 217, 561, 730
Hpy8I GTNNAC 2 cut(s) 185, 594
HpyAV CCTTC 3 cut(s) 24, 53, 154
HpyCH4III ACNGT 4 cut(s) 79, 136, 575, 619
HpyCH4V TGCA 3 cut(s) 569, 578, 600
HpyF10VI GCNNNNNNNGC 2 cut(s) 494, 575
HpyF3I CTNAG 1 cut(s) 72
Hsp92I GRCGYC 1 cut(s) 465
Hsp92II CATG 1 cut(s) 310
HspAI GCGC 1 cut(s) 746
Kzo9I GATC 4 cut(s) 151, 358, 437, 448
LmnI GCTCC 2 cut(s) 129, 457
Lsp1109I GCAGC 3 cut(s) 411, 481, 519
LweI GCATC 1 cut(s) 283
MaeI CTAG 6 cut(s) 234, 297, 377, 474, 707, 844
MaeIII GTNAC 4 cut(s) 263, 276, 407, 503
MalI GATC 4 cut(s) 153, 360, 439, 450
MboI GATC 4 cut(s) 151, 358, 437, 448
MboII GAAGA 2 cut(s) 672, 753
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 1 cut(s) 134
MluCI AATT 5 cut(s) 82, 288, 340, 602, 810
MlyI GAGTC 2 cut(s) 657, 799
MnlI CCTC 6 cut(s) 260, 423, 518, 557, 560, 809
MroXI GAANNNNTTC 1 cut(s) 143
MseI TTAA 5 cut(s) 99, 174, 230, 444, 614
MslI CAYNNNNRTG 2 cut(s) 309, 654
MspA1I CMGCKG 1 cut(s) 488
MspI CCGG 1 cut(s) 536
MspR9I CCNGG 2 cut(s) 254, 462
MvaI CCWGG 2 cut(s) 254, 462
MwoI GCNNNNNNNGC 2 cut(s) 494, 575
NdeII GATC 4 cut(s) 151, 358, 437, 448
NheI GCTAGC 1 cut(s) 296
NlaIII CATG 1 cut(s) 310
NlaIV GGNNCC 2 cut(s) 459, 820
NmuCI GTSAC 2 cut(s) 263, 407
NsbI TGCGCA 1 cut(s) 747
NspI RCATGY 1 cut(s) 310
PaeR7I CTCGAG 1 cut(s) 561
PaqCI CACCTGC 1 cut(s) 605
PciI ACATGT 1 cut(s) 306
PdmI GAANNNNTTC 1 cut(s) 143
PfeI GAWTC 1 cut(s) 8
PkrI GCNGC 4 cut(s) 426, 496, 499, 534
PleI GAGTC 2 cut(s) 657, 799
PpsI GAGTC 2 cut(s) 657, 799
PpuMI RGGWCCY 1 cut(s) 256
PscI ACATGT 1 cut(s) 306
PshBI ATTAAT 2 cut(s) 99, 174
PsiI TTATAA 1 cut(s) 780
Psp5II RGGWCCY 1 cut(s) 256
Psp6I CCWGG 2 cut(s) 252, 460
PspFI CCCAGC 1 cut(s) 631
PspGI CCWGG 2 cut(s) 252, 460
PspN4I GGNNCC 2 cut(s) 459, 820
PspPI GGNCC 2 cut(s) 219, 256
PspPPI RGGWCCY 1 cut(s) 256
PsuI RGATCY 1 cut(s) 448
RsaI GTAC 1 cut(s) 736
RsaNI GTAC 1 cut(s) 735
RseI CAYNNNNRTG 2 cut(s) 309, 654
SaqAI TTAA 5 cut(s) 99, 174, 230, 444, 614
SatI GCNGC 4 cut(s) 425, 495, 498, 533
Sau3AI GATC 4 cut(s) 151, 358, 437, 448
Sau96I GGNCC 2 cut(s) 219, 256
SchI GAGTC 2 cut(s) 657, 799
ScrFI CCNGG 2 cut(s) 254, 462
SduI GDGCHC 1 cut(s) 134
SetI ASST 9 cut(s) 190, 198, 258, 529, 534, 568, 599, 633, 820
SfaNI GCATC 1 cut(s) 283
Sfr274I CTCGAG 1 cut(s) 561
SinI GGWCC 2 cut(s) 219, 256
SlaI CTCGAG 1 cut(s) 561
SmiMI CAYNNNNRTG 2 cut(s) 309, 654
SmlI CTYRAG 1 cut(s) 561
SmoI CTYRAG 1 cut(s) 561
SpeI ACTAGT 1 cut(s) 233
Sse9I AATT 5 cut(s) 82, 288, 340, 602, 810
SsiI CCGC 3 cut(s) 356, 488, 498
SspMI CTAG 6 cut(s) 234, 297, 377, 474, 707, 844
StyD4I CCNGG 2 cut(s) 252, 460
StyI CCWWGG 2 cut(s) 259, 829
TaaI ACNGT 4 cut(s) 79, 136, 575, 619
TaqI TCGA 1 cut(s) 562
TasI AATT 5 cut(s) 82, 288, 340, 602, 810
TauI GCSGC 1 cut(s) 500
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 5 cut(s) 99, 174, 230, 444, 614
Tru9I TTAA 5 cut(s) 99, 174, 230, 444, 614
TscAI CASTG 4 cut(s) 24, 139, 580, 695
TseFI GTSAC 2 cut(s) 263, 407
TseI GCWGC 3 cut(s) 424, 494, 532
Tsp45I GTSAC 2 cut(s) 263, 407
TspDTI ATGAA 3 cut(s) 555, 638, 753
TspRI CASTG 4 cut(s) 24, 139, 580, 695
VpaK11BI GGWCC 2 cut(s) 219, 256
VspI ATTAAT 2 cut(s) 99, 174
XceI RCATGY 1 cut(s) 310
XhoI CTCGAG 1 cut(s) 561
XmiI GTMKAC 1 cut(s) 184
XmnI GAANNNNTTC 1 cut(s) 143
XspI CTAG 6 cut(s) 234, 297, 377, 474, 707, 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.