Rmu_sc0001792.1_g000012
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001792.1
OG0013738
Physical Location & Seq
Forward (+)
42678 .. 47411
4734 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3312 bp
atggtaccatgttttttcttcatttttcttgttctgccatctaagtctgcatttccttgtgaggattccagtgattgcaaaactctgctcaaattcaaggaggagctgacaagtgatcccgaaggtcatcttcaaacttggaacgaagccaatcccttctgctactggactggaattacatgccaccaacgtctccaaaaccgggttataggccttgaactgatagatatgggcttgcaaggaaggatatcaccattcatatccaacctctctcttcttaccaacctgtctttacagagcaatgggttccatggacaaattccatcttccttgggattacttaaagagttggcatttctgaatgtaagctcaaacaagcttaaaggtagcattccaggctcattacatggttgccagagcttgaaagtcatagacttgaatgacaacaatctatctggtgttctccctaaagaactcggctggatgaagaatctaacatacttggctctctccttaaacaatctcagtggactgcttccagcctcactttcaaacttgacggaattgacaaaactacaactggccgtcaactattttactggaaagatcccaccagagcttggagcattaagaaagctagagattttgcatctccatacaaactatctcgaaggctcaatacctgcagtaattggtaactgcactgctttacgcgaaatctcgctgcttgagaatatgttaaatggagaaatcccttcagatatgttttctaaactcaagaatatgcagaaaatgtacatgtcaaacaacaagctttctgggagaattccagtgaccctcaccaatctttcacagctgatactgtttgatgttagtttgaataatttagagggagaagttcctgcaggattgggcaagctagagaaccttgagaatttctacttacataccaacaacttgggtggcggctctggtaattcttctctcagtttcttgactgctctaactaactgttcatatctgcaaaaactacacttgggttcgtgtttgttcactggaaatctaccatcttcaataggtgacctttcaaaagacctctattacttgaatctttttaataaccacattacaggaagtataccagatagaatcggaaacttaagcggcctggtaaccttgaacttgtcatacaaccgtttggaaggaaaaattccatcttctcttggcaggctttcgaatttgcagaggttatacttggggaacaacagaattattgggccaatgccaaaagacttggggatgatgcctaaccttggtttactagaatttggcaacaattcgattggtgggccaattccgccttcacttggtaatctctcacagctgagatatctttacctgtctagaaatcgcctatcaggaaagtttccgattgagcttactcaatgttctcttatcatgctgcttgatctgtctttcaacaacttaaaagggtctgttcctgtacagattgaccgtttttccaatttagctctctctcttaatctttcgaacaataacttcgaaggacagttgcctgcaagcattggaaagctggtatctgtactggcaattgacttgtctgacaaccagttttctgggtccatacctacattgatgggaagctgcatatctttggcgtatctaaacatgtccaacaacatatttgaggggactattccagagtcgttgaagctgatcactcaacttgaagttttggacttgtcacttaatcgattaaatggcagcattccaatctggattgctaaagcacagatgatcaagaatctcaatttatcttataacaggttatcaggagaaattccagacactgggaggataaaatctttcaatagaagctcattcttggggaatgtggggttgtgtggtggttctgcactacttggtcgtccgccatgcgtagttcaaaagagaaaatataagataagaaaatcgatactttactctttggttgcagcagttgcagtagtctgtgctctggttgctgtctttgtttattgtttcttcttcttcagaaaacgggattcaaaaccagaacaccgtgaacacagaatgctaagagtaatgggatcaccaagccatcatggaagtcgaattttcacccaaagggaacttgaaattgcaacaggtgagttcaatgaagctcatctcctgggtagagggaccttcggaacagtctataaagcaattgttgatgatggggatactattatggcagtgaaggttctacatggtgacagtattcagagttacaaaagcttcaagagggaatgtcaaatcatgtccgaaattaagcaccggaatcttgtaagaatggtaggatatacctggaacaaacagttcaaggctcttattctcgagtatattgacaatggtaacttagcacagcatctttatcctggtgaactggaggaaggagcatgtgaattatcgttgagggatagaatgtcaatagctatagatgttgccagtggcttggagtatcttcaggaaggttgtccagtccaaattatgcactgtgatctaaaaccagaaaatgtgcttattgacaacaatatggtggctcatgtagcagatttcgggattgggaagctcaacgcagctgagaaatccaacgaatcacatgttagcacaacacattttctacgtggatcaattggttacattcccccaggtatgcataatcacatcactagtttcacatctataaagtcgtggttaatagagtttacttgtattacagaatatggacaaggaattgaggtatcagcaaaaggagacatctatagctttggtgtcatggtgcttgagatgataacaagaagaagaccgacaagcaatatgtttccggatgggcttgatctaaggaagtgggtggtttcttcgttcccagaccatgtttgggatgttgttgacagtacattaaaggagatacaaagcacacatggcgctttggatgagcttgagaaatgctgcattcaaatgcttgatgtggggttgatgtgcacagaagagaatccacaagaaagacctgctatgtcttttgttgtgcagaagttgaaacaggcgataaggttgagggactggtcgcagatcgagactaaattggccggccggagttctgaagcgagtttagggatgtttccggtgagctttccattttgtttttggatgagacaacgccagctcgttcgccgccgacagcctcagagttcgtcgtcgtcgaattcagtccaggtaagctggacttga

Protein Analysis

1103

Amino Acids

122.47

Weight (kDa)

8.35

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 23 - 61 4.3e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 134 - 244 5.7e-09 Leucine-rich repeat region
LRR_8 PF13855 140 - 197 5.4e-06 Leucine rich repeat
LRR_8 PF13855 211 - 272 7.9e-06 Leucine rich repeat
LRR_14 PF23598 329 - 466 3.3e-12 Leucine-rich repeat region
LRR_8 PF13855 366 - 424 4.6e-08 Leucine rich repeat
Pkinase PF00069 733 - 1035 6.7e-34 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 736 - 1040 2.8e-34 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1842
Acc36I ACCTGC 2 cut(s) 691, 3100
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 2 cut(s) 620, 1871
AccI GTMKAC 1 cut(s) 1138
AccII CGCG 1 cut(s) 714
AccIII TCCGGA 1 cut(s) 2908
AciI CCGC 5 cut(s) 966, 1164, 1358, 1952, 3256
AclWI GGATC 4 cut(s) 110, 601, 2137, 2719
AcoI YGGCCR 3 cut(s) 582, 3168, 3172
AcuI CTGAAG 4 cut(s) 741, 2056, 2531, 3204
AfaI GTAC 5 cut(s) 6, 797, 1506, 1605, 2980
AfiI CCNNNNNNNGG 6 cut(s) 202, 620, 1313, 1367, 1871, 2962
AflII CTTAAG 1 cut(s) 1159
AflIII ACRYGT 3 cut(s) 798, 1689, 2683
AhlI ACTAGT 1 cut(s) 2753
AjnI CCWGG 7 cut(s) 394, 1167, 2211, 2387, 2458, 2731, 3294
AjuI GAANNNNNNNTTGG 2 cut(s) 1345, 1377
Alw21I GWGCWC 2 cut(s) 2038, 3068
Alw26I GTCTC 4 cut(s) 197, 2832, 3152, 3229
Alw44I GTGCAC 1 cut(s) 3064
AlwI GGATC 4 cut(s) 110, 601, 2137, 2719
AlwNI CAGNNNCTG 1 cut(s) 1871
Ama87I CYCGRG 1 cut(s) 2417
Aor13HI TCCGGA 1 cut(s) 2908
AoxI GGCC 7 cut(s) 211, 582, 1165, 1277, 1349, 3168, 3172
ApaLI GTGCAC 1 cut(s) 3064
ApeKI GCWGC 7 cut(s) 724, 1462, 1665, 1785, 2015, 2660, 3033
Asp718I GGTACC 1 cut(s) 4
AspLEI GCGC 1 cut(s) 3011
AspS9I GGNCC 4 cut(s) 1277, 1349, 1641, 2223
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 9 cut(s) 243, 832, 1091, 2124, 2152, 2201, 2306, 2474, 3220
AsuII TTCGAA 3 cut(s) 1235, 1550, 1563
AvaI CYCGRG 1 cut(s) 2417
AvaII GGWCC 2 cut(s) 1641, 2223
BaeGI GKGCMC 1 cut(s) 3068
BanI GGYRCC 1 cut(s) 4
BarI GAAGNNNNNNTAC 2 cut(s) 663, 695
BbsI GAAGAC 1 cut(s) 2893
Bbv12I GWGCWC 2 cut(s) 2038, 3068
BbvI GCAGC 7 cut(s) 711, 1449, 1652, 1797, 2027, 2672, 3020
BccI CCATC 8 cut(s) 46, 331, 1075, 1222, 1651, 2148, 2252, 2906
BceAI ACGGC 1 cut(s) 569
BciT130I CCWGG 7 cut(s) 396, 1169, 2213, 2389, 2460, 2733, 3296
BciVI GTATCC 1 cut(s) 2257
BclI TGATCA 2 cut(s) 1737, 1818
BcnI CCSGG 1 cut(s) 203
BcoDI GTCTC 4 cut(s) 197, 2832, 3152, 3229
BcuI ACTAGT 1 cut(s) 2753
BfaI CTAG 5 cut(s) 638, 920, 1322, 1404, 2754
BfmI CTRYAG 4 cut(s) 684, 903, 2517, 2845
BfoI RGCGCY 1 cut(s) 3012
BfrI CTTAAG 1 cut(s) 1159
BfuAI ACCTGC 2 cut(s) 691, 3100
BfuI GTATCC 1 cut(s) 2257
Bme1390I CCNGG 8 cut(s) 203, 396, 1169, 2213, 2389, 2460, 2733, 3296
Bme18I GGWCC 2 cut(s) 1641, 2223
BmeT110I CYCGRG 1 cut(s) 2417
BmgT120I GGNCC 4 cut(s) 1277, 1349, 1641, 2223
BmiI GGNNCC 4 cut(s) 6, 308, 1642, 2224
BmrFI CCNGG 8 cut(s) 203, 396, 1169, 2213, 2389, 2460, 2733, 3296
BmrI ACTGGG 1 cut(s) 1881
BmsI GCATC 3 cut(s) 658, 1293, 2458
BmuI ACTGGG 1 cut(s) 1881
BpiI GAAGAC 1 cut(s) 2893
BpmI CTGGAG 1 cut(s) 2489
Bpu14I TTCGAA 3 cut(s) 1235, 1550, 1563
BpuEI CTTGAG 5 cut(s) 749, 761, 950, 2888, 3044
BpuMI CCSGG 1 cut(s) 203
Bsa29I ATCGAT 2 cut(s) 1774, 1994
BsaAI YACGTR 1 cut(s) 2708
BsaJI CCNNGG 5 cut(s) 310, 330, 1312, 2212, 2731
BsaWI WCCGGW 3 cut(s) 2358, 2908, 3205
Bsc4I CCNNNNNNNGG 6 cut(s) 202, 620, 1313, 1367, 1871, 2962
Bse118I RCCGGY 1 cut(s) 3170
Bse3DI GCAATG 1 cut(s) 307
BseAI TCCGGA 1 cut(s) 2908
BseBI CCWGG 7 cut(s) 396, 1169, 2213, 2389, 2460, 2733, 3296
BseCI ATCGAT 2 cut(s) 1774, 1994
BseDI CCNNGG 5 cut(s) 310, 330, 1312, 2212, 2731
BseGI GGATG 7 cut(s) 489, 1305, 2917, 2971, 3022, 3204, 3237
BseLI CCNNNNNNNGG 6 cut(s) 202, 620, 1313, 1367, 1871, 2962
BseMI GCAATG 1 cut(s) 307
BseMII CTCAG 5 cut(s) 538, 1000, 1376, 2655, 3281
BseRI GAGGAG 1 cut(s) 116
BseSI GKGCMC 1 cut(s) 3068
BseX3I CGGCCG 1 cut(s) 3172
BseXI GCAGC 7 cut(s) 711, 1449, 1652, 1797, 2027, 2672, 3020
BsgI GTGCAG 3 cut(s) 685, 1920, 3131
Bsh1236I CGCG 1 cut(s) 714
Bsh1285I CGRYCG 1 cut(s) 3175
BshFI GGCC 7 cut(s) 213, 584, 1167, 1279, 1351, 3170, 3174
BshNI GGYRCC 1 cut(s) 4
BshVI ATCGAT 2 cut(s) 1774, 1994
BsiEI CGRYCG 1 cut(s) 3175
BsiHKAI GWGCWC 2 cut(s) 2038, 3068
BsiHKCI CYCGRG 1 cut(s) 2417
BsiSI CCGG 6 cut(s) 202, 2359, 2909, 3171, 3175, 3206
BslFI GGGAC 3 cut(s) 1726, 2236, 3155
BslI CCNNNNNNNGG 6 cut(s) 202, 620, 1313, 1367, 1871, 2962
BsmAI GTCTC 4 cut(s) 197, 2832, 3152, 3229
BsmBI CGTCTC 1 cut(s) 197
BsmFI GGGAC 3 cut(s) 1726, 2236, 3155
BsmI GAATGC 4 cut(s) 390, 1788, 2118, 3036
BsnI GGCC 7 cut(s) 213, 584, 1167, 1279, 1351, 3170, 3174
BsoBI CYCGRG 1 cut(s) 2417
Bsp119I TTCGAA 3 cut(s) 1235, 1550, 1563
Bsp1286I GDGCHC 2 cut(s) 2038, 3068
Bsp13I TCCGGA 1 cut(s) 2908
Bsp1407I TGTACA 2 cut(s) 795, 1504
Bsp19I CCATGG 1 cut(s) 310
BspACI CCGC 5 cut(s) 966, 1164, 1358, 1952, 3256
BspANI GGCC 7 cut(s) 213, 584, 1167, 1279, 1351, 3170, 3174
BspCNI CTCAG 5 cut(s) 537, 999, 1377, 2656, 3280
BspDI ATCGAT 2 cut(s) 1774, 1994
BspEI TCCGGA 1 cut(s) 2908
BspFNI CGCG 1 cut(s) 714
BspLI GGNNCC 4 cut(s) 6, 308, 1642, 2224
BspMAI CTGCAG 2 cut(s) 688, 907
BspMI ACCTGC 2 cut(s) 691, 3100
BspPI GGATC 4 cut(s) 110, 601, 2137, 2719
BspT104I TTCGAA 3 cut(s) 1235, 1550, 1563
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 1159
BsrDI GCAATG 1 cut(s) 307
BsrFI RCCGGY 1 cut(s) 3170
BsrGI TGTACA 2 cut(s) 795, 1504
BssAI RCCGGY 1 cut(s) 3170
BssECI CCNNGG 5 cut(s) 310, 330, 1312, 2212, 2731
BssNAI GTATAC 1 cut(s) 1139
BssT1I CCWWGG 3 cut(s) 310, 330, 1312
Bst1107I GTATAC 1 cut(s) 1139
Bst2UI CCWGG 7 cut(s) 396, 1169, 2213, 2389, 2460, 2733, 3296
Bst6I CTCTTC 2 cut(s) 279, 3066
BstAFI CTTAAG 1 cut(s) 1159
BstAPI GCANNNNNTGC 1 cut(s) 2021
BstAUI TGTACA 2 cut(s) 795, 1504
BstBAI YACGTR 1 cut(s) 2708
BstBI TTCGAA 3 cut(s) 1235, 1550, 1563
BstC8I GCNNGC 7 cut(s) 236, 917, 1229, 1578, 1582, 3172, 3245
BstDEI CTNAG 9 cut(s) 42, 524, 986, 1385, 2117, 2440, 2664, 2924, 3267
BstDSI CCRYGG 1 cut(s) 310
BstEII GGTNACC 2 cut(s) 1079, 1171
BstF5I GGATG 7 cut(s) 489, 1305, 2917, 2971, 3022, 3204, 3237
BstFNI CGCG 1 cut(s) 714
BstH2I RGCGCY 1 cut(s) 3012
BstHHI GCGC 1 cut(s) 3011
BstMAI GTCTC 4 cut(s) 197, 2832, 3152, 3229
BstMCI CGRYCG 1 cut(s) 3175
BstMWI GCNNNNNNNGC 6 cut(s) 396, 1357, 2021, 2042, 2630, 3006
BstNI CCWGG 7 cut(s) 396, 1169, 2213, 2389, 2460, 2733, 3296
BstNSI RCATGY 5 cut(s) 183, 802, 1693, 2484, 2687
BstPI GGTNACC 2 cut(s) 1079, 1171
BstSCI CCNGG 8 cut(s) 201, 394, 1167, 2211, 2387, 2458, 2731, 3294
BstSFI CTRYAG 4 cut(s) 684, 903, 2517, 2845
BstSLI GKGCMC 1 cut(s) 3068
BstUI CGCG 1 cut(s) 714
BstV1I GCAGC 7 cut(s) 711, 1449, 1652, 1797, 2027, 2672, 3020
BstV2I GAAGAC 1 cut(s) 2893
BstX2I RGATCY 1 cut(s) 606
BstXI CCANNNNNNTGG 3 cut(s) 958, 1637, 2536
BstYI RGATCY 1 cut(s) 606
BstZ17I GTATAC 1 cut(s) 1139
BstZI CGGCCG 1 cut(s) 3172
Bsu15I ATCGAT 2 cut(s) 1774, 1994
BsuI GTATCC 1 cut(s) 2257
BsuRI GGCC 7 cut(s) 213, 584, 1167, 1279, 1351, 3170, 3174
BsuTUI ATCGAT 2 cut(s) 1774, 1994
BtgI CCRYGG 1 cut(s) 310
BtsCI GGATG 7 cut(s) 489, 1305, 2917, 2971, 3022, 3204, 3237
BtsI GCAGTG 2 cut(s) 702, 2283
BtsIMutI CAGTG 9 cut(s) 76, 532, 702, 837, 1053, 1869, 2283, 2536, 2575
BveI ACCTGC 2 cut(s) 691, 3100
Cac8I GCNNGC 7 cut(s) 236, 917, 1229, 1578, 1582, 3172, 3245
CaiI CAGNNNCTG 1 cut(s) 1871
CfoI GCGC 1 cut(s) 3011
Cfr10I RCCGGY 1 cut(s) 3170
Cfr13I GGNCC 4 cut(s) 1277, 1349, 1641, 2223
ClaI ATCGAT 2 cut(s) 1774, 1994
Csp6I GTAC 5 cut(s) 5, 796, 1505, 1604, 2979
CspCI CAANNNNNGTGG 4 cut(s) 508, 543, 943, 978
CviQI GTAC 5 cut(s) 5, 796, 1505, 1604, 2979
DdeI CTNAG 9 cut(s) 42, 524, 986, 1385, 2117, 2440, 2664, 2924, 3267
EaeI YGGCCR 3 cut(s) 582, 3168, 3172
EagI CGGCCG 1 cut(s) 3172
Eam1104I CTCTTC 2 cut(s) 279, 3066
EarI CTCTTC 2 cut(s) 279, 3066
EciI GGCGGA 2 cut(s) 1347, 1941
EclXI CGGCCG 1 cut(s) 3172
Eco130I CCWWGG 3 cut(s) 310, 330, 1312
Eco147I AGGCCT 1 cut(s) 213
Eco32I GATATC 2 cut(s) 249, 1391
Eco47I GGWCC 2 cut(s) 1641, 2223
Eco52I CGGCCG 1 cut(s) 3172
Eco57I CTGAAG 4 cut(s) 741, 2056, 2531, 3204
Eco88I CYCGRG 1 cut(s) 2417
Eco91I GGTNACC 2 cut(s) 1079, 1171
EcoO109I RGGNCCY 1 cut(s) 2223
EcoO65I GGTNACC 2 cut(s) 1079, 1171
EcoRI GAATTC 2 cut(s) 825, 3286
EcoRII CCWGG 7 cut(s) 394, 1167, 2211, 2387, 2458, 2731, 3294
EcoRV GATATC 2 cut(s) 249, 1391
EcoT14I CCWWGG 3 cut(s) 310, 330, 1312
EcoT22I ATGCAT 1 cut(s) 2742
ErhI CCWWGG 3 cut(s) 310, 330, 1312
Esp3I CGTCTC 1 cut(s) 197
FalI AAGNNNNNCTT 3 cut(s) 114, 146, 3292
FaqI GGGAC 3 cut(s) 1726, 2236, 3155
FbaI TGATCA 2 cut(s) 1737, 1818
FblI GTMKAC 1 cut(s) 1138
FokI GGATG 7 cut(s) 496, 1312, 2924, 2978, 3029, 3211, 3244
FseI GGCCGGCC 1 cut(s) 3174
FspBI CTAG 5 cut(s) 638, 920, 1322, 1404, 2754
GlaI GCGC 1 cut(s) 3010
GsuI CTGGAG 1 cut(s) 2489
HaeII RGCGCY 1 cut(s) 3012
HaeIII GGCC 7 cut(s) 213, 584, 1167, 1279, 1351, 3170, 3174
HapII CCGG 6 cut(s) 202, 2359, 2909, 3171, 3175, 3206
HhaI GCGC 1 cut(s) 3011
Hin6I GCGC 1 cut(s) 3009
HinP1I GCGC 1 cut(s) 3009
HincII GTYRAC 2 cut(s) 589, 2974
HindII GTYRAC 2 cut(s) 589, 2974
HindIII AAGCTT 3 cut(s) 377, 812, 2317
HpaII CCGG 6 cut(s) 202, 2359, 2909, 3171, 3175, 3206
HphI GGTGA 9 cut(s) 243, 832, 1091, 2124, 2152, 2201, 2306, 2474, 3220
Hpy99I CGWCG 3 cut(s) 3280, 3283, 3286
HpyCH4IV ACGT 2 cut(s) 190, 2707
HpyF10VI GCNNNNNNNGC 6 cut(s) 396, 1357, 2021, 2042, 2630, 3006
HpyF3I CTNAG 9 cut(s) 42, 524, 986, 1385, 2117, 2440, 2664, 2924, 3267
HpySE526I ACGT 2 cut(s) 190, 2707
HspAI GCGC 1 cut(s) 3009
Kpn2I TCCGGA 1 cut(s) 2908
KpnI GGTACC 1 cut(s) 8
KroI GCCGGC 1 cut(s) 3170
KroNI GCCGGC 1 cut(s) 3172
Ksp22I TGATCA 2 cut(s) 1737, 1818
LmnI GCTCC 3 cut(s) 103, 623, 2477
Lsp1109I GCAGC 7 cut(s) 711, 1449, 1652, 1797, 2027, 2672, 3020
LweI GCATC 3 cut(s) 658, 1293, 2458
MaeI CTAG 5 cut(s) 638, 920, 1322, 1404, 2754
MaeII ACGT 2 cut(s) 190, 2707
MaeIII GTNAC 9 cut(s) 695, 832, 1079, 1171, 1764, 2294, 2309, 2435, 2720
MfeI CAATTG 3 cut(s) 1611, 2247, 2715
MflI RGATCY 1 cut(s) 606
MhlI GDGCHC 2 cut(s) 2038, 3068
MlyI GAGTC 1 cut(s) 1733
MmeI TCCRAC 3 cut(s) 288, 1719, 2697
Mph1103I ATGCAT 1 cut(s) 2742
MroI TCCGGA 1 cut(s) 2908
MroNI GCCGGC 1 cut(s) 3170
MslI CAYNNNNRTG 1 cut(s) 3041
MspA1I CMGCKG 3 cut(s) 856, 1384, 2663
MspCI CTTAAG 1 cut(s) 1159
MspI CCGG 6 cut(s) 202, 2359, 2909, 3171, 3175, 3206
MspR9I CCNGG 8 cut(s) 203, 396, 1169, 2213, 2389, 2460, 2733, 3296
MunI CAATTG 3 cut(s) 1611, 2247, 2715
Mva1269I GAATGC 4 cut(s) 390, 1788, 2118, 3036
MvaI CCWGG 7 cut(s) 396, 1169, 2213, 2389, 2460, 2733, 3296
MvnI CGCG 1 cut(s) 714
MwoI GCNNNNNNNGC 6 cut(s) 396, 1357, 2021, 2042, 2630, 3006
NaeI GCCGGC 1 cut(s) 3172
NciI CCSGG 1 cut(s) 203
NcoI CCATGG 1 cut(s) 310
NgoMIV GCCGGC 1 cut(s) 3170
NlaIV GGNNCC 4 cut(s) 6, 308, 1642, 2224
NmeAIII GCCGAG 1 cut(s) 456
NmuCI GTSAC 4 cut(s) 832, 1079, 1764, 2294
NsiI ATGCAT 1 cut(s) 2742
NspI RCATGY 5 cut(s) 183, 802, 1693, 2484, 2687
NspV TTCGAA 3 cut(s) 1235, 1550, 1563
PaeR7I CTCGAG 1 cut(s) 2417
PceI AGGCCT 1 cut(s) 213
PciI ACATGT 3 cut(s) 798, 1689, 2683
PcsI WCGNNNNNNNCGW 1 cut(s) 3281
PctI GAATGC 4 cut(s) 390, 1788, 2118, 3036
PdiI GCCGGC 1 cut(s) 3172
PfeI GAWTC 9 cut(s) 65, 490, 1108, 1149, 1825, 2084, 2362, 2678, 3076
PflMI CCANNNNNTGG 2 cut(s) 620, 1871
PleI GAGTC 1 cut(s) 1732
PpsI GAGTC 1 cut(s) 1732
Ppu21I YACGTR 1 cut(s) 2708
PpuMI RGGWCCY 1 cut(s) 2223
PscI ACATGT 3 cut(s) 798, 1689, 2683
PsiI TTATAA 1 cut(s) 1842
Psp5II RGGWCCY 1 cut(s) 2223
Psp6I CCWGG 7 cut(s) 394, 1167, 2211, 2387, 2458, 2731, 3294
PspEI GGTNACC 2 cut(s) 1079, 1171
PspGI CCWGG 7 cut(s) 394, 1167, 2211, 2387, 2458, 2731, 3294
PspN4I GGNNCC 4 cut(s) 6, 308, 1642, 2224
PspPI GGNCC 4 cut(s) 1277, 1349, 1641, 2223
PspPPI RGGWCCY 1 cut(s) 2223
PsrI GAACNNNNNNTAC 2 cut(s) 1172, 1204
PstI CTGCAG 2 cut(s) 688, 907
PstNI CAGNNNCTG 1 cut(s) 1871
PsuI RGATCY 1 cut(s) 606
PvuII CAGCTG 3 cut(s) 856, 1384, 2663
RigI GGCCGGCC 1 cut(s) 3174
RsaI GTAC 5 cut(s) 6, 797, 1506, 1605, 2980
RsaNI GTAC 5 cut(s) 5, 796, 1505, 1604, 2979
RseI CAYNNNNRTG 1 cut(s) 3041
Sau96I GGNCC 4 cut(s) 1277, 1349, 1641, 2223
SbfI CCTGCAGG 1 cut(s) 907
SchI GAGTC 1 cut(s) 1733
ScrFI CCNGG 8 cut(s) 203, 396, 1169, 2213, 2389, 2460, 2733, 3296
SdaI CCTGCAGG 1 cut(s) 907
SduI GDGCHC 2 cut(s) 2038, 3068
SfaNI GCATC 3 cut(s) 658, 1293, 2458
SfcI CTRYAG 4 cut(s) 684, 903, 2517, 2845
Sfr274I CTCGAG 1 cut(s) 2417
SfuI TTCGAA 3 cut(s) 1235, 1550, 1563
SinI GGWCC 2 cut(s) 1641, 2223
SlaI CTCGAG 1 cut(s) 2417
SmiMI CAYNNNNRTG 1 cut(s) 3041
SmlI CTYRAG 7 cut(s) 728, 776, 929, 1159, 2417, 2867, 3023
SmoI CTYRAG 7 cut(s) 728, 776, 929, 1159, 2417, 2867, 3023
SpeI ACTAGT 1 cut(s) 2753
Sse8387I CCTGCAGG 1 cut(s) 907
SseBI AGGCCT 1 cut(s) 213
SsiI CCGC 5 cut(s) 966, 1164, 1358, 1952, 3256
SspMI CTAG 5 cut(s) 638, 920, 1322, 1404, 2754
StuI AGGCCT 1 cut(s) 213
StyD4I CCNGG 8 cut(s) 201, 394, 1167, 2211, 2387, 2458, 2731, 3294
StyI CCWWGG 3 cut(s) 310, 330, 1312
TaiI ACGT 2 cut(s) 193, 2710
TaqII GACCGA 1 cut(s) 2905
TatI WGTACW 4 cut(s) 795, 1504, 1603, 2978
TauI GCSGC 3 cut(s) 969, 1167, 3258
TfiI GAWTC 9 cut(s) 65, 490, 1108, 1149, 1825, 2084, 2362, 2678, 3076
TscAI CASTG 9 cut(s) 76, 532, 709, 837, 1060, 1876, 2283, 2536, 2582
TseFI GTSAC 4 cut(s) 832, 1079, 1764, 2294
TseI GCWGC 7 cut(s) 724, 1462, 1665, 1785, 2015, 2660, 3033
Tsp45I GTSAC 4 cut(s) 832, 1079, 1764, 2294
TspDTI ATGAA 5 cut(s) 10, 247, 500, 1005, 2214
TspGWI ACGGA 1 cut(s) 575
TspRI CASTG 9 cut(s) 76, 532, 709, 837, 1060, 1876, 2283, 2536, 2582
Van91I CCANNNNNTGG 2 cut(s) 620, 1871
Vha464I CTTAAG 1 cut(s) 1159
VneI GTGCAC 1 cut(s) 3064
VpaK11BI GGWCC 2 cut(s) 1641, 2223
XbaI TCTAGA 1 cut(s) 1403
XceI RCATGY 5 cut(s) 183, 802, 1693, 2484, 2687
XcmI CCANNNNNNNNNTGG 2 cut(s) 2958, 3225
XhoI CTCGAG 1 cut(s) 2417
XmiI GTMKAC 1 cut(s) 1138
XspI CTAG 5 cut(s) 638, 920, 1322, 1404, 2754
Zsp2I ATGCAT 1 cut(s) 2742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.