Rmu_sc0001850.1_g000050

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001850.1
OG0007277
Physical Location & Seq
Forward (+)
193625 .. 201588
7964 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1293 bp
atgaagtctgctctcctcctcctctcagcttttgctgctctcctcctcgccattttcgcttctctcaatcccaaccgccagtggttcagttggaggtcaccggcggcggacttggtggtgaagaatgccaagatatacacagtcgacgagtctctccccttcgccgattccatggccgttcgcaacggaaggattctccggattggaagctattcctcagttcaggatatggtagggtatgggactaaagaattagatcttaagggaaaaattgtagtccctggatttattgattcccatgtgcatctgatctttggtggactgcagatgatgcgtgtagaactacatggcataaactcaaaagatgaatttgtgaagaggatcaaagaagctgtgagaaatgcaaaacaaggttcctggattttgggcggtggatggaacaatgatctatggggaggtgagttgccattggcttcttggattgacgatattacaccttataatcctgtctttctatcaagaatggatgggcatatgagcttggctaattcagtggcactcaagttgggtgggattactaaatcatcagtggatccaaatggtggaaccatcatgaaaaccaccagtggagaacctactggactgttgattgattctgcaatggaacatatccttccactcattccagaggtctcagtagaggagcggagggaagctttgcgtaaagccagcaatcatgccttgatgaggggtgtgacgacagttgttgacgttggtagatactttcctgggacttctgtaaaagattcctgggaagatttttcagatgtgtatcaatgggctgatttgtcagggaagatgactatcagggtctgcttatttttcccactggagacttggtcacgtttacgtgatcttatcaacaatgttggccaatctatgagccagtggatttatttgggtggggttaaagcatttgctgatgggtcattgggttctcgtagcgcacttttctatgagcatctggcacctgggacaccagccagattcggtaaactagggattgttgcttcagtacaggttgatcacactactccttgggctcagagatcttatttattcaagtcactcttgggtagcaatgtagaattggcatttggctccgactggccagttgtagacattaatcctttaggtggtattaagacggcaatgaaaaggatgcccccggcttgggatattgcttgggttccatcagagagcgtttctctgaatgactcactgaaagcgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_3

Protein Analysis

430

Amino Acids

47.23

Weight (kDa)

7.81

Isoelectric Point (pI)

34.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_3 PF07969 84 - 343 7e-42 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 501
AccB1I GGYRCC 1 cut(s) 1027
AccB7I CCANNNNNTGG 1 cut(s) 602
AccBSI CCGCTC 1 cut(s) 706
AccI GTMKAC 2 cut(s) 144, 1179
AccIII TCCGGA 1 cut(s) 198
AciI CCGC 5 cut(s) 76, 104, 107, 429, 706
AclWI GGATC 3 cut(s) 389, 587, 600
AcoI YGGCCR 3 cut(s) 174, 931, 1169
AcsI RAATTY 1 cut(s) 368
AcuI CTGAAG 1 cut(s) 1056
AfaI GTAC 1 cut(s) 1077
AfiI CCNNNNNNNGG 5 cut(s) 602, 747, 1196, 1233, 1234
AflII CTTAAG 1 cut(s) 260
AgsI TTSAA 1 cut(s) 1123
AjnI CCWGG 5 cut(s) 280, 416, 787, 809, 1030
AjuI GAANNNNNNNTTGG 2 cut(s) 1140, 1172
AluBI AGCT 5 cut(s) 29, 210, 392, 540, 716
AluI AGCT 5 cut(s) 29, 210, 392, 540, 716
Alw26I GTCTC 3 cut(s) 156, 697, 887
AlwI GGATC 3 cut(s) 389, 587, 600
AlwNI CAGNNNCTG 1 cut(s) 873
Aor13HI TCCGGA 1 cut(s) 198
AoxI GGCC 3 cut(s) 174, 931, 1169
ApeKI GCWGC 1 cut(s) 35
ApoI RAATTY 1 cut(s) 368
AseI ATTAAT 1 cut(s) 1185
Asp700I GAANNNNTTC 1 cut(s) 211
AspLEI GCGC 1 cut(s) 1007
AsuC2I CCSGG 1 cut(s) 1229
AsuHPI GGTGA 3 cut(s) 90, 130, 470
BalI TGGCCA 2 cut(s) 933, 1171
BamHI GGATCC 1 cut(s) 592
BanI GGYRCC 1 cut(s) 1027
BanII GRGCYC 1 cut(s) 1105
BbvI GCAGC 1 cut(s) 22
BccI CCATC 5 cut(s) 429, 521, 617, 977, 1261
BceAI ACGGC 2 cut(s) 161, 1224
BciT130I CCWGG 5 cut(s) 282, 418, 789, 811, 1032
BclI TGATCA 1 cut(s) 1084
BcnI CCSGG 1 cut(s) 1229
BcoDI GTCTC 3 cut(s) 156, 697, 887
BfaI CTAG 1 cut(s) 1058
BfmI CTRYAG 1 cut(s) 323
BfrI CTTAAG 1 cut(s) 260
BglII AGATCT 2 cut(s) 256, 1109
BisI GCNGC 2 cut(s) 36, 105
BlsI GCNGC 2 cut(s) 37, 106
Bme1390I CCNGG 6 cut(s) 282, 418, 789, 811, 1032, 1229
BmiI GGNNCC 6 cut(s) 415, 594, 607, 1029, 1162, 1251
BmrFI CCNGG 6 cut(s) 282, 418, 789, 811, 1032, 1229
BmsI GCATC 4 cut(s) 313, 321, 1030, 1212
BplI GAGNNNNNCTC 2 cut(s) 1252, 1284
BpmI CTGGAG 1 cut(s) 911
BpuEI CTTGAG 1 cut(s) 545
BpuMI CCSGG 1 cut(s) 1229
BsaAI YACGTR 1 cut(s) 911
BsaBI GATNNNNATC 2 cut(s) 831, 863
BsaI GGTCTC 1 cut(s) 697
BsaJI CCNNGG 7 cut(s) 171, 280, 788, 810, 1031, 1097, 1227
BsaWI WCCGGW 1 cut(s) 198
BsaXI ACNNNNNCTCC 2 cut(s) 884, 914
Bsc4I CCNNNNNNNGG 5 cut(s) 602, 747, 1196, 1233, 1234
Bse118I RCCGGY 1 cut(s) 100
Bse1I ACTGG 7 cut(s) 79, 624, 643, 894, 946, 1172, 1172
Bse3DI GCAATG 3 cut(s) 666, 1147, 1218
Bse8I GATNNNNATC 2 cut(s) 831, 863
BseAI TCCGGA 1 cut(s) 198
BseBI CCWGG 5 cut(s) 282, 418, 789, 811, 1032
BseDI CCNNGG 7 cut(s) 171, 280, 788, 810, 1031, 1097, 1227
BseGI GGATG 3 cut(s) 440, 532, 1227
BseJI GATNNNNATC 2 cut(s) 831, 863
BseLI CCNNNNNNNGG 5 cut(s) 602, 747, 1196, 1233, 1234
BseMI GCAATG 3 cut(s) 666, 1147, 1218
BseMII CTCAG 4 cut(s) 39, 231, 708, 1118
BseNI ACTGG 7 cut(s) 79, 624, 643, 894, 946, 1172, 1172
BseRI GAGGAG 6 cut(s) 5, 8, 11, 32, 35, 716
BseXI GCAGC 1 cut(s) 22
BshFI GGCC 3 cut(s) 176, 933, 1171
BshNI GGYRCC 1 cut(s) 1027
BsiSI CCGG 3 cut(s) 101, 199, 1229
BslFI GGGAC 4 cut(s) 256, 263, 805, 1048
BslI CCNNNNNNNGG 5 cut(s) 602, 747, 1196, 1233, 1234
BsmAI GTCTC 3 cut(s) 156, 697, 887
BsmFI GGGAC 4 cut(s) 256, 263, 805, 1048
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 3 cut(s) 176, 933, 1171
Bso31I GGTCTC 1 cut(s) 697
Bsp1286I GDGCHC 1 cut(s) 1105
Bsp13I TCCGGA 1 cut(s) 198
Bsp143I GATC 8 cut(s) 256, 309, 381, 445, 592, 913, 1084, 1109
Bsp19I CCATGG 1 cut(s) 171
BspACI CCGC 5 cut(s) 76, 104, 107, 429, 706
BspANI GGCC 3 cut(s) 176, 933, 1171
BspCNI CTCAG 4 cut(s) 38, 230, 707, 1117
BspEI TCCGGA 1 cut(s) 198
BspHI TCATGA 1 cut(s) 612
BspLI GGNNCC 6 cut(s) 415, 594, 607, 1029, 1162, 1251
BspMAI CTGCAG 1 cut(s) 327
BspPI GGATC 3 cut(s) 389, 587, 600
BspT107I GGYRCC 1 cut(s) 1027
BspTI CTTAAG 1 cut(s) 260
BspTNI GGTCTC 1 cut(s) 697
BsrBI CCGCTC 1 cut(s) 706
BsrDI GCAATG 3 cut(s) 666, 1147, 1218
BsrFI RCCGGY 1 cut(s) 100
BsrI ACTGG 7 cut(s) 79, 624, 643, 894, 946, 1172, 1172
BssAI RCCGGY 1 cut(s) 100
BssECI CCNNGG 7 cut(s) 171, 280, 788, 810, 1031, 1097, 1227
BssMI GATC 8 cut(s) 256, 309, 381, 445, 592, 913, 1084, 1109
BssT1I CCWWGG 2 cut(s) 171, 1097
Bst2UI CCWGG 5 cut(s) 282, 418, 789, 811, 1032
Bst4CI ACNGT 3 cut(s) 142, 645, 763
Bst6I CTCTTC 1 cut(s) 371
BstAFI CTTAAG 1 cut(s) 260
BstAPI GCANNNNNTGC 1 cut(s) 331
BstBAI YACGTR 1 cut(s) 911
BstC8I GCNNGC 1 cut(s) 730
BstDEI CTNAG 4 cut(s) 25, 217, 694, 1104
BstDSI CCRYGG 1 cut(s) 171
BstEII GGTNACC 1 cut(s) 96
BstENI CCTNNNNNAGG 1 cut(s) 745
BstF5I GGATG 3 cut(s) 440, 532, 1227
BstHHI GCGC 1 cut(s) 1007
BstKTI GATC 8 cut(s) 259, 312, 384, 448, 595, 916, 1087, 1112
BstMAI GTCTC 3 cut(s) 156, 697, 887
BstMBI GATC 8 cut(s) 256, 309, 381, 445, 592, 913, 1084, 1109
BstMWI GCNNNNNNNGC 3 cut(s) 35, 56, 331
BstNI CCWGG 5 cut(s) 282, 418, 789, 811, 1032
BstPI GGTNACC 1 cut(s) 96
BstSCI CCNGG 6 cut(s) 280, 416, 787, 809, 1030, 1227
BstSFI CTRYAG 1 cut(s) 323
BstV1I GCAGC 1 cut(s) 22
BstX2I RGATCY 3 cut(s) 256, 592, 1109
BstYI RGATCY 3 cut(s) 256, 592, 1109
BsuRI GGCC 3 cut(s) 176, 933, 1171
BtgI CCRYGG 1 cut(s) 171
BtsCI GGATG 3 cut(s) 440, 532, 1227
BtsIMutI CAGTG 7 cut(s) 86, 558, 594, 631, 887, 953, 1280
Cac8I GCNNGC 1 cut(s) 730
CaiI CAGNNNCTG 1 cut(s) 873
CciI TCATGA 1 cut(s) 612
CfoI GCGC 1 cut(s) 1007
Cfr10I RCCGGY 1 cut(s) 100
Csp6I GTAC 1 cut(s) 1076
CspCI CAANNNNNGTGG 2 cut(s) 550, 585
CviAII CATG 5 cut(s) 172, 299, 347, 613, 737
CviQI GTAC 1 cut(s) 1076
DdeI CTNAG 4 cut(s) 25, 217, 694, 1104
DpnI GATC 8 cut(s) 258, 311, 383, 447, 594, 915, 1086, 1111
DpnII GATC 8 cut(s) 256, 309, 381, 445, 592, 913, 1084, 1109
EaeI YGGCCR 3 cut(s) 174, 931, 1169
Eam1104I CTCTTC 1 cut(s) 371
EarI CTCTTC 1 cut(s) 371
EciI GGCGGA 1 cut(s) 122
Eco130I CCWWGG 2 cut(s) 171, 1097
Eco24I GRGCYC 1 cut(s) 1105
Eco31I GGTCTC 1 cut(s) 697
Eco57I CTGAAG 1 cut(s) 1056
Eco91I GGTNACC 1 cut(s) 96
EcoNI CCTNNNNNAGG 1 cut(s) 745
EcoO65I GGTNACC 1 cut(s) 96
EcoRII CCWGG 5 cut(s) 280, 416, 787, 809, 1030
EcoT14I CCWWGG 2 cut(s) 171, 1097
EcoT38I GRGCYC 1 cut(s) 1105
ErhI CCWWGG 2 cut(s) 171, 1097
FaeI CATG 5 cut(s) 175, 302, 350, 616, 740
FalI AAGNNNNNCTT 2 cut(s) 1115, 1147
FaqI GGGAC 4 cut(s) 256, 263, 805, 1048
FatI CATG 5 cut(s) 171, 298, 346, 612, 736
FauNDI CATATG 1 cut(s) 534
FbaI TGATCA 1 cut(s) 1084
FblI GTMKAC 2 cut(s) 144, 1179
Fnu4HI GCNGC 2 cut(s) 36, 105
FokI GGATG 3 cut(s) 447, 539, 1234
FriOI GRGCYC 1 cut(s) 1105
Fsp4HI GCNGC 2 cut(s) 36, 105
FspBI CTAG 1 cut(s) 1058
GlaI GCGC 1 cut(s) 1006
GluI GCNGC 2 cut(s) 36, 105
GsuI CTGGAG 1 cut(s) 911
HaeIII GGCC 3 cut(s) 176, 933, 1171
HapII CCGG 3 cut(s) 101, 199, 1229
HhaI GCGC 1 cut(s) 1007
Hin1II CATG 5 cut(s) 175, 302, 350, 616, 740
Hin6I GCGC 1 cut(s) 1005
HinP1I GCGC 1 cut(s) 1005
HincII GTYRAC 2 cut(s) 145, 769
HindII GTYRAC 2 cut(s) 145, 769
HindIII AAGCTT 1 cut(s) 714
HinfI GANTC 8 cut(s) 149, 167, 193, 293, 653, 806, 1047, 1277
HpaII CCGG 3 cut(s) 101, 199, 1229
HphI GGTGA 3 cut(s) 90, 130, 470
Hpy166II GTNNAC 6 cut(s) 145, 320, 769, 908, 1055, 1180
Hpy188I TCNGA 6 cut(s) 309, 826, 1107, 1165, 1258, 1272
Hpy188III TCNNGA 5 cut(s) 199, 224, 519, 613, 686
Hpy8I GTNNAC 6 cut(s) 145, 320, 769, 908, 1055, 1180
Hpy99I CGWCG 1 cut(s) 149
HpyAV CCTTC 3 cut(s) 169, 183, 683
HpyCH4III ACNGT 3 cut(s) 142, 645, 763
HpyCH4IV ACGT 3 cut(s) 771, 904, 910
HpyCH4V TGCA 4 cut(s) 304, 325, 404, 659
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 56, 331
HpyF3I CTNAG 4 cut(s) 25, 217, 694, 1104
HpySE526I ACGT 3 cut(s) 771, 904, 910
Hsp92II CATG 5 cut(s) 175, 302, 350, 616, 740
HspAI GCGC 1 cut(s) 1005
Kpn2I TCCGGA 1 cut(s) 198
Ksp22I TGATCA 1 cut(s) 1084
Kzo9I GATC 8 cut(s) 256, 309, 381, 445, 592, 913, 1084, 1109
LmnI GCTCC 2 cut(s) 703, 1166
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 4 cut(s) 313, 321, 1030, 1212
MaeI CTAG 1 cut(s) 1058
MaeII ACGT 3 cut(s) 771, 904, 910
MaeIII GTNAC 4 cut(s) 96, 754, 900, 1125
MalI GATC 8 cut(s) 258, 311, 383, 447, 594, 915, 1086, 1111
MbiI CCGCTC 1 cut(s) 706
MboI GATC 8 cut(s) 256, 309, 381, 445, 592, 913, 1084, 1109
MboII GAAGA 4 cut(s) 133, 388, 827, 868
MflI RGATCY 3 cut(s) 256, 592, 1109
MhlI GDGCHC 1 cut(s) 1105
MlsI TGGCCA 2 cut(s) 933, 1171
MluCI AATT 5 cut(s) 251, 270, 368, 547, 1148
MluNI TGGCCA 2 cut(s) 933, 1171
MlyI GAGTC 2 cut(s) 158, 1271
MmeI TCCRAC 2 cut(s) 71, 1188
Mox20I TGGCCA 2 cut(s) 933, 1171
MroI TCCGGA 1 cut(s) 198
MroXI GAANNNNTTC 1 cut(s) 211
MscI TGGCCA 2 cut(s) 933, 1171
MseI TTAA 4 cut(s) 261, 969, 1185, 1203
Msp20I TGGCCA 2 cut(s) 933, 1171
MspCI CTTAAG 1 cut(s) 260
MspI CCGG 3 cut(s) 101, 199, 1229
MspR9I CCNGG 6 cut(s) 282, 418, 789, 811, 1032, 1229
Mva1269I GAATGC 1 cut(s) 130
MvaI CCWGG 5 cut(s) 282, 418, 789, 811, 1032
MwoI GCNNNNNNNGC 3 cut(s) 35, 56, 331
NciI CCSGG 1 cut(s) 1229
NcoI CCATGG 1 cut(s) 171
NdeI CATATG 1 cut(s) 534
NdeII GATC 8 cut(s) 256, 309, 381, 445, 592, 913, 1084, 1109
NlaIII CATG 5 cut(s) 175, 302, 350, 616, 740
NlaIV GGNNCC 6 cut(s) 415, 594, 607, 1029, 1162, 1251
NmuCI GTSAC 4 cut(s) 96, 754, 900, 1125
PagI TCATGA 1 cut(s) 612
PctI GAATGC 1 cut(s) 130
PdmI GAANNNNTTC 1 cut(s) 211
PfeI GAWTC 6 cut(s) 167, 193, 293, 653, 806, 1047
PflFI GACNNNGTC 1 cut(s) 898
PflMI CCANNNNNTGG 1 cut(s) 602
PfoI TCCNGGA 1 cut(s) 416
PkrI GCNGC 2 cut(s) 37, 106
PleI GAGTC 2 cut(s) 157, 1271
PpsI GAGTC 2 cut(s) 157, 1271
Ppu21I YACGTR 1 cut(s) 911
PshBI ATTAAT 1 cut(s) 1185
PsiI TTATAA 1 cut(s) 501
Psp6I CCWGG 5 cut(s) 280, 416, 787, 809, 1030
PspEI GGTNACC 1 cut(s) 96
PspGI CCWGG 5 cut(s) 280, 416, 787, 809, 1030
PspN4I GGNNCC 6 cut(s) 415, 594, 607, 1029, 1162, 1251
PstI CTGCAG 1 cut(s) 327
PstNI CAGNNNCTG 1 cut(s) 873
PsuI RGATCY 3 cut(s) 256, 592, 1109
PsyI GACNNNGTC 1 cut(s) 898
RsaI GTAC 1 cut(s) 1077
RsaNI GTAC 1 cut(s) 1076
SalI GTCGAC 1 cut(s) 143
SaqAI TTAA 4 cut(s) 261, 969, 1185, 1203
SatI GCNGC 2 cut(s) 36, 105
Sau3AI GATC 8 cut(s) 256, 309, 381, 445, 592, 913, 1084, 1109
SchI GAGTC 2 cut(s) 158, 1271
ScrFI CCNGG 6 cut(s) 282, 418, 789, 811, 1032, 1229
SduI GDGCHC 1 cut(s) 1105
SfaNI GCATC 4 cut(s) 313, 321, 1030, 1212
SfcI CTRYAG 1 cut(s) 323
SgrAI CRCCGGYG 1 cut(s) 100
SmlI CTYRAG 2 cut(s) 260, 560
SmoI CTYRAG 2 cut(s) 260, 560
Sse9I AATT 5 cut(s) 251, 270, 368, 547, 1148
SsiI CCGC 5 cut(s) 76, 104, 107, 429, 706
SspMI CTAG 1 cut(s) 1058
StyD4I CCNGG 6 cut(s) 280, 416, 787, 809, 1030, 1227
StyI CCWWGG 2 cut(s) 171, 1097
TaaI ACNGT 3 cut(s) 142, 645, 763
TaiI ACGT 3 cut(s) 774, 907, 913
TaqI TCGA 1 cut(s) 144
TasI AATT 5 cut(s) 251, 270, 368, 547, 1148
TatI WGTACW 1 cut(s) 1075
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 6 cut(s) 167, 193, 293, 653, 806, 1047
Tru1I TTAA 4 cut(s) 261, 969, 1185, 1203
Tru9I TTAA 4 cut(s) 261, 969, 1185, 1203
TscAI CASTG 7 cut(s) 86, 558, 594, 631, 894, 953, 1287
TseFI GTSAC 4 cut(s) 96, 754, 900, 1125
TseI GCWGC 1 cut(s) 35
Tsp45I GTSAC 4 cut(s) 96, 754, 900, 1125
TspDTI ATGAA 4 cut(s) 17, 381, 629, 1229
TspGWI ACGGA 1 cut(s) 201
TspRI CASTG 7 cut(s) 86, 558, 594, 631, 894, 953, 1287
Tth111I GACNNNGTC 1 cut(s) 898
Van91I CCANNNNNTGG 1 cut(s) 602
Vha464I CTTAAG 1 cut(s) 260
VspI ATTAAT 1 cut(s) 1185
XagI CCTNNNNNAGG 1 cut(s) 745
XapI RAATTY 1 cut(s) 368
XcmI CCANNNNNNNNNTGG 2 cut(s) 474, 894
XmiI GTMKAC 2 cut(s) 144, 1179
XmnI GAANNNNTTC 1 cut(s) 211
XspI CTAG 1 cut(s) 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.