Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0001955.1

Orthogroup ID

OG0010361

Physical Location & Seq

Genomic Coordinates Reverse (-)

35908 .. 39585

Gene Structure 3678 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2622 bp

atggcgtcaacgccgccgcactgttcaatcacagcgaccaagccgtaccaaacccaccaatacccacaaaaccagcgcctcaaatctcagcggcagacccgtcagagcaaccaatggaccacccaccacgtctctctctcaaagcctctacctttacccccgcaaccgccgcgcacggcggcgaaacccgccgccgccgctcccagtccgacctccttttcctctctctgccctccgtccaagtccgacctcgtcgccgccttctcgggccgccgctcgacccggttcgtgtccaaaatgcacttcggccggcccaaaaccacggtggggtcccgccactcctcgctggcggaggaggccctgcagagcgcaatccagttcggtaaggacgatttggcccttgatgatgttttgcttagtttcgagtctaggctttgtgggtcggatgattacacttttttgcttagagagcttgggaatagaggtgagtgttggaaggctatgaggtgtttcgagtttgcggtaaggagggagagaaggagaaccgagcaggggaaattagctagctctatgattagtactcttggtaggttggggaaggttgagcttgccaagaatgtgtttcagactgctgtcaacgagggttatggcaagactgtttatacctattcggctttgattagtgcttatgggcgcagcgggtattgcgatgaggctattcgagtgctcgagtccatgaaagattcgggcttgaagccgaatttggttacttacaatgcggtgattgatgcatgtggaaaagggggagtggagtttaagaaggtagtggagattttcaatgagatgcttaaaattggggtgcagcctgatcgaattacttataattctctccttgcggtttgtagtcgagggggactgtgggaggcggctagaaacttgttcagtgagatggtggatagagggattgatcaggatatttatacctataatacacttttggatgcaatttctaaaggtgggaagatggatttggcatatcagattatgtcggagatgccttcaaagaatattttgcctaatgtggtgacttatagtactatgatcgatggttatgctaaggctggtagattagaagatgcactcagtttatttaatgaaatgaagtttttggccattggtctggatagagttctgtataatacattgctgtcactttacgggaagcttggcaggtttgcagaggctttgaatgtttgcaaggagatggagagtgttgggattgtgaaggacgttgtatcttataatgcacttcttggtgggtatgggaagcaaggcaagtatgatcaagttaaagcactgtacaatgaaatgaaagaagaacgtgtatcaccaaatttattaacttattcaacattgatcgatgtgtattcaaaaggtggtttgtatgtggaggcaatgaaagttttcagagagtttaagcaggcagggctgaaggctgatgttgttctctatagtgaacttattaatgctttgtgcaaaaacgggctggtggaatctgctgtttcattgcttgatgagatgaccaaggaaggcatccgacctaatgttgtcacttataattctataattgatgcctttggtaggccagcgacaacagagtgtgcagttggtgctggtgctggaggcattgtgctacacaatgaatcttcatcttcaatttctgaaagggatgttgacatttctgaagagaatgttcaaattcaggtgagagatatggaggataccagaattataaagatgtttgggcagcttgcagctgataaagctggttatgcagagaaagataggaaggttagacaagaaatcttgtgcattttgggtgtcgttcagaagatgcatgagctagacatcaaaccgaatgtcgtcacattttcagccattctaaatgcttgcagccgctgtaattcatttgaagatgcttccatgttattggaggaattgcggttgtttgataaccaggtttatggtgttgcccatggacttcttatgggctacaggggtagtgtatgggttaaagctcagtctctgtttgatgaagttaagcagatggactgttcaactgcatctgcattctacaatgctcttaccgacatgctatggcactttggacagaaacaaggggcgcaactggttgtgcttgaagggaagcgtcgaaatgtatgggagaatgcatgggagaattcacagttagatttgcatttgatgtcttctggggctgcccgagcaatggttcatgcatggttgctcaatatacactctatagtgtataagggccaacaattgccaaacctattaagcattttgacaggatggggcaagcacagcaaagtagtaggcgatagtgccttaagacgagcaatcgaggcacttcttaccggcatgggtgcaccctttcaggttgccaagcctaacaaaggtaggtttatttcgacgggatctgtggctgctgcatggttaaaagaatctggcacactggaggtgctcaagcttcatgatgatagggctgatccaaatagtgcaaattttggccaaatatccaatttacgagcgctcgctttgtag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010019 GO:0016070 GO:0016787 GO:0016788 GO:0023052 GO:0031930 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3 Smr

Protein Analysis

873

Amino Acids

96.46

Weight (kDa)

8.64

Isoelectric Point (pI)

37.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	191 - 283	4.1e-09	Fungal tetratrico peptide repeats
PPR_3	PF13812	207 - 267	6.9e-13	Pentatricopeptide repeat domain
PPR_2	PF13041	219 - 267	3.4e-13	PPR repeat family
PPR_1	PF12854	219 - 247	4e-06	PPR repeat
PPR	PF01535	221 - 251	7.2e-08	PPR repeat
PPR_2	PF13041	292 - 337	1.4e-11	PPR repeat family
PPR	PF01535	292 - 322	1.4e-06	PPR repeat
PPR_long	PF17177	301 - 450	4.6e-10	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	313 - 371	4.1e-13	Pentatricopeptide repeat domain
PPR_2	PF13041	325 - 373	5.2e-16	PPR repeat family
PPR_3	PF13812	348 - 408	1.7e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	355 - 388	1.2e-13	PPR repeat
PPR_2	PF13041	359 - 389	2.4e-10	PPR repeat family
PPR	PF01535	362 - 388	4.8e-10	PPR repeat
PPR	PF01535	397 - 427	9.2e-06	PPR repeat
PPR_3	PF13812	418 - 477	5.8e-13	Pentatricopeptide repeat domain
PPR_2	PF13041	429 - 477	8.5e-16	PPR repeat family
PPR	PF01535	432 - 461	5.7e-07	PPR repeat
PPR	PF01535	468 - 497	3.3e-06	PPR repeat
TPR_24	PF23276	485 - 548	2.2e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	487 - 547	1.2e-13	Pentatricopeptide repeat domain
PPR_1	PF12854	496 - 528	6.1e-10	PPR repeat
PPR_2	PF13041	500 - 547	6.6e-16	PPR repeat family
PPR	PF01535	502 - 532	5.8e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010361)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31400 AT2G31400
fragaria_vesca	FvH4_1g27040 FvH4_1g27040 FvH4_1g27040 FvH4_1g27040 FvH4_1g27040
malus_domestica	MD09G1284400.v1.1 MD17G1277600.v1.1
prunus_persica	Prupe.3G009900_v2.0.a1 Prupe.3G010400_v2.0.a1 Prupe.3G010400_v2.0.a1
pyrus_communis	pycom09g19230 pycom17g27520
rosa_chinensis	RchiOBHm_Chr3g0487361
rosa_laevigata	RLG00000023011
rosa_multiflora	Rmu_sc0001955.1_g000008
rosa_roxburghii	Rroxscaffold_6G00394850
rosa_rugosa	Rorug03G0233500
rosa_samantha	Rh3DG313000
rosa_wichuraiana	Rw3G024920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	882, 1302, 1617, 1793
Acc36I	ACCTGC	1 cut(s)	1221
AccBSI	CCGCTC	2 cut(s)	200, 276
AccII	CGCG	1 cut(s)	172
AclWI	GGATC	2 cut(s)	2503, 2561
AcoI	YGGCCR	3 cut(s)	307, 1170, 2587
AcsI	RAATTY	5 cut(s)	760, 1393, 1758, 2239, 2581
AcuI	CTGAAG	2 cut(s)	1511, 1764
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	4 cut(s)	47, 580, 1096, 1361
AfeI	AGCGCT	1 cut(s)	2610
AfiI	CCNNNNNNNGG	5 cut(s)	327, 552, 1641, 2287, 2474
AflII	CTTAAG	1 cut(s)	2407
AflIII	ACRYGT	1 cut(s)	1381
AjiI	CACGTC	1 cut(s)	130
AjnI	CCWGG	1 cut(s)	2016
AjuI	GAANNNNNNNTTGG	6 cut(s)	287, 319, 746, 778, 1013, 1045
Alw21I	GWGCWC	3 cut(s)	729, 2449, 2544
Alw26I	GTCTC	2 cut(s)	136, 2088
Alw44I	GTGCAC	1 cut(s)	2445
AlwI	GGATC	2 cut(s)	2503, 2561
AlwNI	CAGNNNCTG	2 cut(s)	1959, 2086
Ama87I	CYCGRG	3 cut(s)	265, 728, 2280
Aor51HI	AGCGCT	1 cut(s)	2610
AoxI	GGCC	9 cut(s)	268, 307, 311, 357, 396, 1170, 1643, 2332, 2587
ApaLI	GTGCAC	1 cut(s)	2445
ApeKI	GCWGC	8 cut(s)	696, 862, 1807, 1814, 1953, 2276, 2504, 2507
ApoI	RAATTY	5 cut(s)	760, 1393, 1758, 2239, 2581
ArsI	GACNNNNNNTTYG	2 cut(s)	1905, 1937
AseI	ATTAAT	1 cut(s)	1524
Asp700I	GAANNNNTTC	1 cut(s)	1463
AspLEI	GCGC	6 cut(s)	78, 174, 371, 696, 2185, 2611
AspS9I	GGNCC	7 cut(s)	117, 268, 312, 330, 358, 397, 2332
AsuC2I	CCSGG	1 cut(s)	283
AsuHPI	GGTGA	5 cut(s)	497, 793, 1096, 1380, 1777
AsuNHI	GCTAGC	1 cut(s)	563
AvaI	CYCGRG	3 cut(s)	265, 728, 2280
AvaII	GGWCC	2 cut(s)	117, 330
BaeGI	GKGCMC	1 cut(s)	2449
BalI	TGGCCA	2 cut(s)	1172, 2589
BbsI	GAAGAC	1 cut(s)	2259
Bbv12I	GWGCWC	3 cut(s)	729, 2449, 2544
BbvI	GCAGC	8 cut(s)	708, 874, 1819, 1826, 1965, 2263, 2491, 2494
BccI	CCATC	6 cut(s)	943, 1018, 1100, 1258, 2101, 2364
BceAI	ACGGC	2 cut(s)	28, 192
BcgI	CGANNNNNNTGC	4 cut(s)	851, 885, 1094, 1128
BciT130I	CCWGG	1 cut(s)	2018
BciVI	GTATCC	1 cut(s)	1774
BclI	TGATCA	2 cut(s)	967, 1342
BcnI	CCSGG	1 cut(s)	283
BcoDI	GTCTC	2 cut(s)	136, 2088
BfaI	CTAG	4 cut(s)	429, 564, 930, 1904
BfmI	CTRYAG	4 cut(s)	362, 1510, 2053, 2319
BfoI	RGCGCY	2 cut(s)	79, 2612
BfrI	CTTAAG	1 cut(s)	2407
BfuAI	ACCTGC	1 cut(s)	1221
BfuI	GTATCC	1 cut(s)	1774
BmcAI	AGTACT	2 cut(s)	580, 1096
Bme1390I	CCNGG	2 cut(s)	283, 2018
Bme18I	GGWCC	1 cut(s)	117
BmeT110I	CYCGRG	3 cut(s)	265, 728, 2280
BmgBI	CACGTC	1 cut(s)	130
BmgT120I	GGNCC	7 cut(s)	117, 268, 312, 330, 358, 397, 2332
BmiI	GGNNCC	2 cut(s)	331, 332
BmrFI	CCNGG	2 cut(s)	283, 2018
BmrI	ACTGGG	1 cut(s)	198
BmtI	GCTAGC	1 cut(s)	567
BmuI	ACTGGG	1 cut(s)	198
BoxI	GACNNNNGTC	1 cut(s)	632
BpiI	GAAGAC	1 cut(s)	2259
BpmI	CTGGAG	2 cut(s)	1701, 2555
Bpu10I	CCTNAGC	1 cut(s)	1116
BpuEI	CTTGAG	1 cut(s)	2528
BpuMI	CCSGG	1 cut(s)	283
Bsa29I	ATCGAT	2 cut(s)	1104, 1419
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	3 cut(s)	321, 1584, 2035
BsaXI	ACNNNNNCTCC	2 cut(s)	1674, 1704
Bsc4I	CCNNNNNNNGG	5 cut(s)	327, 552, 1641, 2287, 2474
Bse118I	RCCGGY	2 cut(s)	309, 2435
Bse1I	ACTGG	4 cut(s)	204, 376, 2193, 2538
Bse3DI	GCAATG	4 cut(s)	1202, 1461, 1565, 2292
BseBI	CCWGG	1 cut(s)	2018
BseCI	ATCGAT	2 cut(s)	1104, 1419
BseDI	CCNNGG	3 cut(s)	321, 1584, 2035
BseGI	GGATG	5 cut(s)	451, 1006, 1593, 1735, 2375
BseLI	CCNNNNNNNGG	5 cut(s)	327, 552, 1641, 2287, 2474
BseMI	GCAATG	4 cut(s)	1202, 1461, 1565, 2292
BseMII	CTCAG	3 cut(s)	101, 1156, 2093
BseNI	ACTGG	4 cut(s)	204, 376, 2193, 2538
BseRI	GAGGAG	2 cut(s)	331, 368
BseSI	GKGCMC	1 cut(s)	2449
BseX3I	CGGCCG	1 cut(s)	307
BseXI	GCAGC	8 cut(s)	708, 874, 1819, 1826, 1965, 2263, 2491, 2494
BsgI	GTGCAG	2 cut(s)	881, 1683
Bsh1236I	CGCG	1 cut(s)	172
Bsh1285I	CGRYCG	1 cut(s)	310
BshFI	GGCC	9 cut(s)	270, 309, 313, 359, 398, 1172, 1645, 2334, 2589
BshVI	ATCGAT	2 cut(s)	1104, 1419
BsiEI	CGRYCG	1 cut(s)	310
BsiHKAI	GWGCWC	3 cut(s)	729, 2449, 2544
BsiHKCI	CYCGRG	3 cut(s)	265, 728, 2280
BsiSI	CCGG	3 cut(s)	283, 310, 2436
BslFI	GGGAC	2 cut(s)	316, 927
BslI	CCNNNNNNNGG	5 cut(s)	327, 552, 1641, 2287, 2474
BsmAI	GTCTC	2 cut(s)	136, 2088
BsmBI	CGTCTC	1 cut(s)	136
BsmFI	GGGAC	2 cut(s)	316, 927
BsmI	GAATGC	2 cut(s)	2129, 2233
BsnI	GGCC	9 cut(s)	270, 309, 313, 359, 398, 1172, 1645, 2334, 2589
BsoBI	CYCGRG	3 cut(s)	265, 728, 2280
Bsp1286I	GDGCHC	3 cut(s)	729, 2449, 2544
Bsp1407I	TGTACA	1 cut(s)	1359
Bsp143I	GATC	7 cut(s)	868, 967, 1101, 1342, 1416, 2495, 2566
Bsp19I	CCATGG	1 cut(s)	2035
BspANI	GGCC	9 cut(s)	270, 309, 313, 359, 398, 1172, 1645, 2334, 2589
BspCNI	CTCAG	3 cut(s)	100, 1155, 2092
BspDI	ATCGAT	2 cut(s)	1104, 1419
BspFNI	CGCG	1 cut(s)	172
BspHI	TCATGA	1 cut(s)	2551
BspLI	GGNNCC	2 cut(s)	331, 332
BspMAI	CTGCAG	1 cut(s)	366
BspMI	ACCTGC	1 cut(s)	1221
BspOI	GCTAGC	1 cut(s)	567
BspPI	GGATC	2 cut(s)	2503, 2561
BspTI	CTTAAG	1 cut(s)	2407
BsrBI	CCGCTC	2 cut(s)	200, 276
BsrDI	GCAATG	4 cut(s)	1202, 1461, 1565, 2292
BsrFI	RCCGGY	2 cut(s)	309, 2435
BsrGI	TGTACA	1 cut(s)	1359
BsrI	ACTGG	4 cut(s)	204, 376, 2193, 2538
BssAI	RCCGGY	2 cut(s)	309, 2435
BssECI	CCNNGG	3 cut(s)	321, 1584, 2035
BssMI	GATC	7 cut(s)	868, 967, 1101, 1342, 1416, 2495, 2566
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	2 cut(s)	1584, 2035
Bst2UI	CCWGG	1 cut(s)	2018
Bst4CI	ACNGT	7 cut(s)	23, 325, 658, 918, 1359, 2114, 2247
Bst6I	CTCTTC	1 cut(s)	1740
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	1 cut(s)	2407
BstAPI	GCANNNNNTGC	1 cut(s)	1670
BstAUI	TGTACA	1 cut(s)	1359
BstDEI	CTNAG	6 cut(s)	87, 416, 464, 1116, 1142, 2079
BstDSI	CCRYGG	2 cut(s)	321, 2035
BstENI	CCTNNNNNAGG	2 cut(s)	1639, 2472
BstF5I	GGATG	5 cut(s)	451, 1006, 1593, 1735, 2375
BstFNI	CGCG	1 cut(s)	172
BstH2I	RGCGCY	2 cut(s)	79, 2612
BstHHI	GCGC	6 cut(s)	78, 174, 371, 696, 2185, 2611
BstKTI	GATC	7 cut(s)	871, 970, 1104, 1345, 1419, 2498, 2569
BstMAI	GTCTC	2 cut(s)	136, 2088
BstMBI	GATC	7 cut(s)	868, 967, 1101, 1342, 1416, 2495, 2566
BstMCI	CGRYCG	1 cut(s)	310
BstNI	CCWGG	1 cut(s)	2018
BstNSI	RCATGY	2 cut(s)	795, 2155
BstPAI	GACNNNNGTC	1 cut(s)	632
BstSCI	CCNGG	2 cut(s)	281, 2016
BstSFI	CTRYAG	4 cut(s)	362, 1510, 2053, 2319
BstSLI	GKGCMC	1 cut(s)	2449
BstUI	CGCG	1 cut(s)	172
BstV1I	GCAGC	8 cut(s)	708, 874, 1819, 1826, 1965, 2263, 2491, 2494
BstV2I	GAAGAC	1 cut(s)	2259
BstX2I	RGATCY	1 cut(s)	2495
BstXI	CCANNNNNNTGG	3 cut(s)	1180, 1990, 2024
BstYI	RGATCY	1 cut(s)	2495
BstZI	CGGCCG	1 cut(s)	307
Bsu15I	ATCGAT	2 cut(s)	1104, 1419
BsuI	GTATCC	1 cut(s)	1774
BsuRI	GGCC	9 cut(s)	270, 309, 313, 359, 398, 1172, 1645, 2334, 2589
BsuTUI	ATCGAT	2 cut(s)	1104, 1419
BtgI	CCRYGG	2 cut(s)	321, 2035
BtgZI	GCGATG	1 cut(s)	723
BtrI	CACGTC	1 cut(s)	130
BtsCI	GGATG	5 cut(s)	451, 1006, 1593, 1735, 2375
BtsIMutI	CAGTG	4 cut(s)	19, 949, 1355, 2531
BveI	ACCTGC	1 cut(s)	1221
CaiI	CAGNNNCTG	2 cut(s)	1959, 2086
CciI	TCATGA	1 cut(s)	2551
CfoI	GCGC	6 cut(s)	78, 174, 371, 696, 2185, 2611
Cfr10I	RCCGGY	2 cut(s)	309, 2435
Cfr13I	GGNCC	7 cut(s)	117, 268, 312, 330, 358, 397, 2332
ClaI	ATCGAT	2 cut(s)	1104, 1419
CseI	GACGC	1 cut(s)	2198
CsiI	ACCWGGT	1 cut(s)	2016
Csp6I	GTAC	4 cut(s)	46, 579, 1095, 1360
CviQI	GTAC	4 cut(s)	46, 579, 1095, 1360
DdeI	CTNAG	6 cut(s)	87, 416, 464, 1116, 1142, 2079
DpnI	GATC	7 cut(s)	870, 969, 1103, 1344, 1418, 2497, 2568
DpnII	GATC	7 cut(s)	868, 967, 1101, 1342, 1416, 2495, 2566
EaeI	YGGCCR	3 cut(s)	307, 1170, 2587
EagI	CGGCCG	1 cut(s)	307
Eam1104I	CTCTTC	1 cut(s)	1740
EarI	CTCTTC	1 cut(s)	1740
EciI	GGCGGA	1 cut(s)	365
EclXI	CGGCCG	1 cut(s)	307
Eco130I	CCWWGG	2 cut(s)	1584, 2035
Eco47I	GGWCC	2 cut(s)	117, 330
Eco47III	AGCGCT	1 cut(s)	2610
Eco52I	CGGCCG	1 cut(s)	307
Eco57I	CTGAAG	2 cut(s)	1511, 1764
Eco88I	CYCGRG	3 cut(s)	265, 728, 2280
EcoNI	CCTNNNNNAGG	2 cut(s)	1639, 2472
EcoO109I	RGGNCCY	2 cut(s)	330, 358
EcoRI	GAATTC	1 cut(s)	2239
EcoRII	CCWGG	1 cut(s)	2016
EcoT14I	CCWWGG	2 cut(s)	1584, 2035
EcoT22I	ATGCAT	4 cut(s)	793, 1899, 2233, 2299
ErhI	CCWWGG	2 cut(s)	1584, 2035
Esp3I	CGTCTC	1 cut(s)	136
FaqI	GGGAC	2 cut(s)	316, 927
FauI	CCCGC	4 cut(s)	168, 196, 341, 692
FbaI	TGATCA	2 cut(s)	967, 1342
FokI	GGATG	5 cut(s)	458, 1013, 1580, 1742, 2382
FseI	GGCCGGCC	1 cut(s)	313
FspBI	CTAG	4 cut(s)	429, 564, 930, 1904
GlaI	GCGC	6 cut(s)	77, 173, 370, 695, 2184, 2610
GsuI	CTGGAG	2 cut(s)	1701, 2555
HaeII	RGCGCY	2 cut(s)	79, 2612
HaeIII	GGCC	9 cut(s)	270, 309, 313, 359, 398, 1172, 1645, 2334, 2589
HapII	CCGG	3 cut(s)	283, 310, 2436
HgaI	GACGC	1 cut(s)	2198
HhaI	GCGC	6 cut(s)	78, 174, 371, 696, 2185, 2611
Hin1I	GRCGYC	1 cut(s)	5
Hin6I	GCGC	6 cut(s)	76, 172, 369, 694, 2183, 2609
HinP1I	GCGC	6 cut(s)	76, 172, 369, 694, 2183, 2609
HincII	GTYRAC	3 cut(s)	9, 637, 1735
HindII	GTYRAC	3 cut(s)	9, 637, 1735
HindIII	AAGCTT	2 cut(s)	1223, 2546
HinfI	GANTC	6 cut(s)	425, 731, 743, 1553, 1703, 2522
HpaII	CCGG	3 cut(s)	283, 310, 2436
HphI	GGTGA	5 cut(s)	497, 793, 1096, 1380, 1777
Hpy166II	GTNNAC	5 cut(s)	9, 637, 1517, 1735, 2447
Hpy188III	TCNNGA	3 cut(s)	971, 1181, 2552
Hpy8I	GTNNAC	5 cut(s)	9, 637, 1517, 1735, 2447
Hpy99I	CGWCG	3 cut(s)	257, 2214, 2494
HpyCH4III	ACNGT	7 cut(s)	23, 325, 658, 918, 1359, 2114, 2247
HpyCH4IV	ACGT	3 cut(s)	129, 1290, 1381
HpyF3I	CTNAG	6 cut(s)	87, 416, 464, 1116, 1142, 2079
HpySE526I	ACGT	3 cut(s)	129, 1290, 1381
Hsp92I	GRCGYC	1 cut(s)	5
HspAI	GCGC	6 cut(s)	76, 172, 369, 694, 2183, 2609
KflI	GGGWCCC	1 cut(s)	330
KroI	GCCGGC	1 cut(s)	309
KroNI	GCCGGC	1 cut(s)	311
Ksp22I	TGATCA	2 cut(s)	967, 1342
Kzo9I	GATC	7 cut(s)	868, 967, 1101, 1342, 1416, 2495, 2566
LmnI	GCTCC	1 cut(s)	205
Lsp1109I	GCAGC	8 cut(s)	708, 874, 1819, 1826, 1965, 2263, 2491, 2494
MabI	ACCWGGT	1 cut(s)	2016
MaeI	CTAG	4 cut(s)	429, 564, 930, 1904
MaeII	ACGT	3 cut(s)	129, 1290, 1381
MaeIII	GTNAC	5 cut(s)	766, 1084, 1209, 1609, 1924
MalI	GATC	7 cut(s)	870, 969, 1103, 1344, 1418, 2497, 2568
MbiI	CCGCTC	2 cut(s)	200, 276
MboI	GATC	7 cut(s)	868, 967, 1101, 1342, 1416, 2495, 2566
MboII	GAAGA	9 cut(s)	1033, 1145, 1388, 1698, 1704, 1757, 1903, 1985, 2259
MfeI	CAATTG	1 cut(s)	2339
MflI	RGATCY	1 cut(s)	2495
MhlI	GDGCHC	3 cut(s)	729, 2449, 2544
MlsI	TGGCCA	2 cut(s)	1172, 2589
MluNI	TGGCCA	2 cut(s)	1172, 2589
MlyI	GAGTC	2 cut(s)	434, 740
MmeI	TCCRAC	6 cut(s)	233, 270, 423, 473, 1029, 1621
Mox20I	TGGCCA	2 cut(s)	1172, 2589
Mph1103I	ATGCAT	4 cut(s)	793, 1899, 2233, 2299
MroNI	GCCGGC	1 cut(s)	309
MroXI	GAANNNNTTC	1 cut(s)	1463
MscI	TGGCCA	2 cut(s)	1172, 2589
Msp20I	TGGCCA	2 cut(s)	1172, 2589
MspA1I	CMGCKG	4 cut(s)	91, 699, 1817, 1959
MspCI	CTTAAG	1 cut(s)	2407
MspI	CCGG	3 cut(s)	283, 310, 2436
MspR9I	CCNGG	2 cut(s)	283, 2018
MunI	CAATTG	1 cut(s)	2339
Mva1269I	GAATGC	2 cut(s)	2129, 2233
MvaI	CCWGG	1 cut(s)	2018
MvnI	CGCG	1 cut(s)	172
NaeI	GCCGGC	1 cut(s)	311
NciI	CCSGG	1 cut(s)	283
NcoI	CCATGG	1 cut(s)	2035
NdeII	GATC	7 cut(s)	868, 967, 1101, 1342, 1416, 2495, 2566
NgoMIV	GCCGGC	1 cut(s)	309
NheI	GCTAGC	1 cut(s)	563
NlaIV	GGNNCC	2 cut(s)	331, 332
NmuCI	GTSAC	4 cut(s)	1084, 1209, 1609, 1924
NsiI	ATGCAT	4 cut(s)	793, 1899, 2233, 2299
NspI	RCATGY	2 cut(s)	795, 2155
PaeR7I	CTCGAG	1 cut(s)	728
PagI	TCATGA	1 cut(s)	2551
PcsI	WCGNNNNNNNCGW	1 cut(s)	387
PctI	GAATGC	2 cut(s)	2129, 2233
PdiI	GCCGGC	1 cut(s)	311
PdmI	GAANNNNTTC	1 cut(s)	1463
PfeI	GAWTC	4 cut(s)	743, 1553, 1703, 2522
PflFI	GACNNNGTC	1 cut(s)	251
PleI	GAGTC	2 cut(s)	433, 739
PpsI	GAGTC	2 cut(s)	433, 739
PpuMI	RGGWCCY	1 cut(s)	330
PshAI	GACNNNNGTC	1 cut(s)	632
PshBI	ATTAAT	1 cut(s)	1524
PsiI	TTATAA	4 cut(s)	882, 1302, 1617, 1793
Psp5II	RGGWCCY	1 cut(s)	330
Psp6I	CCWGG	1 cut(s)	2016
PspGI	CCWGG	1 cut(s)	2016
PspN4I	GGNNCC	2 cut(s)	331, 332
PspPI	GGNCC	7 cut(s)	117, 268, 312, 330, 358, 397, 2332
PspPPI	RGGWCCY	1 cut(s)	330
PspXI	VCTCGAGB	1 cut(s)	728
PstI	CTGCAG	1 cut(s)	366
PstNI	CAGNNNCTG	2 cut(s)	1959, 2086
PsuI	RGATCY	1 cut(s)	2495
PsyI	GACNNNGTC	1 cut(s)	251
PvuII	CAGCTG	1 cut(s)	1817
RigI	GGCCGGCC	1 cut(s)	313
RsaI	GTAC	4 cut(s)	47, 580, 1096, 1361
RsaNI	GTAC	4 cut(s)	46, 579, 1095, 1360
Sau3AI	GATC	7 cut(s)	868, 967, 1101, 1342, 1416, 2495, 2566
Sau96I	GGNCC	7 cut(s)	117, 268, 312, 330, 358, 397, 2332
ScaI	AGTACT	2 cut(s)	580, 1096
SchI	GAGTC	2 cut(s)	434, 740
ScrFI	CCNGG	1 cut(s)	283
SduI	GDGCHC	3 cut(s)	729, 2449, 2544
SexAI	ACCWGGT	1 cut(s)	2016
SfcI	CTRYAG	4 cut(s)	362, 1510, 2053, 2319
Sfr274I	CTCGAG	1 cut(s)	728
SinI	GGWCC	2 cut(s)	117, 330
SlaI	CTCGAG	1 cut(s)	728
SmlI	CTYRAG	3 cut(s)	728, 2407, 2543
SmoI	CTYRAG	3 cut(s)	728, 2407, 2543
SspI	AATATT	1 cut(s)	1069
SspMI	CTAG	4 cut(s)	429, 564, 930, 1904
StyD4I	CCNGG	2 cut(s)	281, 2016
StyI	CCWWGG	2 cut(s)	1584, 2035
TaaI	ACNGT	7 cut(s)	23, 325, 658, 918, 1359, 2114, 2247
TaiI	ACGT	3 cut(s)	132, 1293, 1384
TatI	WGTACW	3 cut(s)	578, 1094, 1359
TfiI	GAWTC	4 cut(s)	743, 1553, 1703, 2522
TscAI	CASTG	4 cut(s)	26, 949, 1362, 2538
TseFI	GTSAC	4 cut(s)	1084, 1209, 1609, 1924
TseI	GCWGC	8 cut(s)	696, 862, 1807, 1814, 1953, 2276, 2504, 2507
Tsp45I	GTSAC	4 cut(s)	1084, 1209, 1609, 1924
TspGWI	ACGGA	1 cut(s)	225
TspRI	CASTG	4 cut(s)	26, 949, 1362, 2538
Tth111I	GACNNNGTC	1 cut(s)	251
Vha464I	CTTAAG	1 cut(s)	2407
VneI	GTGCAC	1 cut(s)	2445
VpaK11BI	GGWCC	2 cut(s)	117, 330
VspI	ATTAAT	1 cut(s)	1524
XagI	CCTNNNNNAGG	2 cut(s)	1639, 2472
XapI	RAATTY	5 cut(s)	760, 1393, 1758, 2239, 2581
XceI	RCATGY	2 cut(s)	795, 2155
XcmI	CCANNNNNNNNNTGG	1 cut(s)	322
XhoI	CTCGAG	1 cut(s)	728
XmnI	GAANNNNTTC	1 cut(s)	1463
XspI	CTAG	4 cut(s)	429, 564, 930, 1904
ZrmI	AGTACT	2 cut(s)	580, 1096
Zsp2I	ATGCAT	4 cut(s)	793, 1899, 2233, 2299

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.