Rmu_sc0002119.1_g000023

B3 domain-containing transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002119.1
OG0011010
Physical Location & Seq
Reverse (-)
113851 .. 114984
1134 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1134 bp
atggagtactttgtgcaaggagaccaaaaagggtattctgataaagaagaggatcaagaagaggaagaagaagagaagaaccacatcattaacagtaagtacttacctccttcttacaattatgggtcatcatcctcctcctcccaaaccccagaagccataaacttcatggacttgtcactaagaaatgcgaaagacaacaacaacaacgattggcccccaaacagtgagcgagagcacatgtttgacaaagttgttactccaagtgatgtgggcaagctcaaccgtctagtgatcccaaaacaacatgccgagaagtacttcccacttgattcgtccaccaacgagaagggtctgttattaaacttccaggaccggactgggaagccatggcggttcaggtattcttactggaacagtagccagagctatgtgatgaccaaaggctggagccggtttgtcaaggacaagaagcttgatgctggtgacattgtgtccttcgagcggagtgtaagggactcaaatgtcttgtacattgactggaaacgccgccctccgcctcctctaccgcctaatcaaacttctctctggggaggcggagggcgattttactcgctgcagcggcctccactaatgtcaatgccactgcggcatcacgagcattttcattacaacattcaccatccataccatcaccagtatcagtatcatcagatgaacccctaccaattgcagcagcagcagcagcagcaacaacaacaatactaccaccacagaggaattagtcccgccgtggtgattgattcagtgccactggtccaacatcatcaccatcagcatggtaaaaatatgagtcctactactaataagcggcttaggctgtttggtgtgaacatggagtacagtccaatgcaagaagatgaaccagaacaatgtcagatattgtcttccacagtggcttcgctctctccacatcaacaacatcatcatcatcatcaccttccttcttcttcgctttctcaacctctggagttaaggctgcccaattatgctgccccactggccggttcagcggatcagctgctttctgaaaagggaaaatctacttccttgtcgttttatttggatccctaa

Protein Analysis

377

Amino Acids

44.02

Weight (kDa)

6.82

Isoelectric Point (pI)

64.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 82 - 183 7.5e-27 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 524
AccB7I CCANNNNNTGG 1 cut(s) 447
AccBSI CCGCTC 1 cut(s) 505
AclWI GGATC 4 cut(s) 60, 289, 1083, 1121
AcoI YGGCCR 1 cut(s) 1062
AfaI GTAC 5 cut(s) 8, 101, 320, 533, 902
AfiI CCNNNNNNNGG 3 cut(s) 447, 504, 1064
AflIII ACRYGT 1 cut(s) 240
AhdI GACNNNNNGTC 1 cut(s) 493
AjnI CCWGG 1 cut(s) 369
AluBI AGCT 4 cut(s) 280, 429, 475, 1081
AluI AGCT 4 cut(s) 280, 429, 475, 1081
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 1 cut(s) 15
AlwI GGATC 4 cut(s) 60, 289, 1083, 1121
AoxI GGCC 3 cut(s) 215, 623, 1062
Asp700I GAANNNNTTC 1 cut(s) 320
AspS9I GGNCC 3 cut(s) 216, 373, 817
AsuHPI GGTGA 6 cut(s) 497, 671, 686, 808, 821, 989
AvaII GGWCC 2 cut(s) 373, 817
BamHI GGATCC 1 cut(s) 1126
BauI CACGAG 1 cut(s) 656
BbsI GAAGAC 1 cut(s) 939
Bbv12I GWGCWC 1 cut(s) 240
BccI CCATC 3 cut(s) 690, 699, 840
BceAI ACGGC 1 cut(s) 776
BciT130I CCWGG 1 cut(s) 371
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 617
BglI GCCNNNNNGGC 2 cut(s) 649, 1061
BmcAI AGTACT 3 cut(s) 8, 101, 320
Bme1390I CCNGG 1 cut(s) 371
Bme18I GGWCC 2 cut(s) 373, 817
BmeRI GACNNNNNGTC 1 cut(s) 493
BmgT120I GGNCC 3 cut(s) 216, 373, 817
BmiI GGNNCC 3 cut(s) 218, 452, 1128
BmrFI CCNGG 1 cut(s) 371
BmrI ACTGGG 1 cut(s) 390
BmsI GCATC 2 cut(s) 469, 661
BmuI ACTGGG 1 cut(s) 390
BpiI GAAGAC 1 cut(s) 939
BpmI CTGGAG 2 cut(s) 469, 1049
Bpu10I CCTNAGC 1 cut(s) 875
BsaI GGTCTC 1 cut(s) 15
BsaJI CCNNGG 2 cut(s) 389, 792
BsaWI WCCGGW 1 cut(s) 375
Bsc4I CCNNNNNNNGG 3 cut(s) 447, 504, 1064
Bse118I RCCGGY 2 cut(s) 453, 1064
Bse1I ACTGG 6 cut(s) 385, 416, 545, 697, 819, 1065
BseBI CCWGG 1 cut(s) 371
BseDI CCNNGG 2 cut(s) 389, 792
BseGI GGATG 2 cut(s) 131, 682
BseLI CCNNNNNNNGG 3 cut(s) 447, 504, 1064
BseNI ACTGG 6 cut(s) 385, 416, 545, 697, 819, 1065
BseRI GAGGAG 3 cut(s) 127, 130, 552
BshFI GGCC 3 cut(s) 217, 625, 1064
BsiHKAI GWGCWC 1 cut(s) 240
BsiSI CCGG 3 cut(s) 376, 454, 1065
BslFI GGGAC 2 cut(s) 530, 771
BslI CCNNNNNNNGG 3 cut(s) 447, 504, 1064
BsmAI GTCTC 1 cut(s) 15
BsmFI GGGAC 2 cut(s) 530, 771
BsnI GGCC 3 cut(s) 217, 625, 1064
Bso31I GGTCTC 1 cut(s) 15
Bsp1286I GDGCHC 1 cut(s) 240
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 4 cut(s) 52, 294, 1075, 1126
Bsp19I CCATGG 1 cut(s) 389
BspANI GGCC 3 cut(s) 217, 625, 1064
BspLI GGNNCC 3 cut(s) 218, 452, 1128
BspMAI CTGCAG 1 cut(s) 621
BspPI GGATC 4 cut(s) 60, 289, 1083, 1121
BspTNI GGTCTC 1 cut(s) 15
BsrBI CCGCTC 1 cut(s) 505
BsrFI RCCGGY 2 cut(s) 453, 1064
BsrGI TGTACA 1 cut(s) 531
BsrI ACTGG 6 cut(s) 385, 416, 545, 697, 819, 1065
BssAI RCCGGY 2 cut(s) 453, 1064
BssECI CCNNGG 2 cut(s) 389, 792
BssMI GATC 4 cut(s) 52, 294, 1075, 1126
BssSI CACGAG 1 cut(s) 656
BssT1I CCWWGG 1 cut(s) 389
Bst2BI CACGAG 1 cut(s) 656
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 6 cut(s) 95, 227, 287, 419, 905, 955
Bst6I CTCTTC 3 cut(s) 42, 54, 66
BstAUI TGTACA 1 cut(s) 531
BstC8I GCNNGC 1 cut(s) 278
BstDEI CTNAG 2 cut(s) 182, 875
BstDSI CCRYGG 2 cut(s) 389, 792
BstF5I GGATG 2 cut(s) 131, 682
BstKTI GATC 4 cut(s) 55, 297, 1078, 1129
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 4 cut(s) 52, 294, 1075, 1126
BstNI CCWGG 1 cut(s) 371
BstNSI RCATGY 2 cut(s) 244, 311
BstSCI CCNGG 1 cut(s) 369
BstSFI CTRYAG 1 cut(s) 617
BstV2I GAAGAC 1 cut(s) 939
BstX2I RGATCY 1 cut(s) 1126
BstXI CCANNNNNNTGG 1 cut(s) 839
BstYI RGATCY 1 cut(s) 1126
BsuRI GGCC 3 cut(s) 217, 625, 1064
BtgI CCRYGG 2 cut(s) 389, 792
BtsCI GGATG 2 cut(s) 131, 682
BtsI GCAGTG 1 cut(s) 644
BtsIMutI CAGTG 6 cut(s) 232, 644, 812, 813, 960, 1058
Cac8I GCNNGC 1 cut(s) 278
Cfr10I RCCGGY 2 cut(s) 453, 1064
Cfr13I GGNCC 3 cut(s) 216, 373, 817
Csp6I GTAC 5 cut(s) 7, 100, 319, 532, 901
CspCI CAANNNNNGTGG 2 cut(s) 252, 287
CviAII CATG 6 cut(s) 169, 241, 308, 390, 839, 895
CviQI GTAC 5 cut(s) 7, 100, 319, 532, 901
DdeI CTNAG 2 cut(s) 182, 875
DpnI GATC 4 cut(s) 54, 296, 1077, 1128
DpnII GATC 4 cut(s) 52, 294, 1075, 1126
DrdI GACNNNNNNGTC 1 cut(s) 524
DriI GACNNNNNGTC 1 cut(s) 493
DseDI GACNNNNNNGTC 1 cut(s) 524
EaeI YGGCCR 1 cut(s) 1062
Eam1104I CTCTTC 3 cut(s) 42, 54, 66
Eam1105I GACNNNNNGTC 1 cut(s) 493
EarI CTCTTC 3 cut(s) 42, 54, 66
EciI GGCGGA 2 cut(s) 546, 612
Eco130I CCWWGG 1 cut(s) 389
Eco31I GGTCTC 1 cut(s) 15
Eco47I GGWCC 2 cut(s) 373, 817
EcoRII CCWGG 1 cut(s) 369
EcoT14I CCWWGG 1 cut(s) 389
ErhI CCWWGG 1 cut(s) 389
FaeI CATG 6 cut(s) 172, 244, 311, 393, 842, 898
FaqI GGGAC 2 cut(s) 530, 771
FatI CATG 6 cut(s) 168, 240, 307, 389, 838, 894
FauI CCCGC 1 cut(s) 796
FokI GGATG 2 cut(s) 118, 669
FspBI CTAG 1 cut(s) 290
GsuI CTGGAG 2 cut(s) 469, 1049
HaeIII GGCC 3 cut(s) 217, 625, 1064
HapII CCGG 3 cut(s) 376, 454, 1065
Hin1II CATG 6 cut(s) 172, 244, 311, 393, 842, 898
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 4 cut(s) 332, 518, 803, 853
HpaII CCGG 3 cut(s) 376, 454, 1065
HphI GGTGA 6 cut(s) 497, 671, 686, 808, 821, 989
Hpy166II GTNNAC 2 cut(s) 339, 892
Hpy188I TCNGA 4 cut(s) 40, 714, 939, 1090
Hpy188III TCNNGA 3 cut(s) 56, 656, 1028
Hpy8I GTNNAC 2 cut(s) 339, 892
HpyAV CCTTC 5 cut(s) 120, 343, 508, 1010, 1014
HpyCH4III ACNGT 6 cut(s) 95, 227, 287, 419, 905, 955
HpyCH4V TGCA 4 cut(s) 16, 619, 733, 913
HpyF3I CTNAG 2 cut(s) 182, 875
Hsp92II CATG 6 cut(s) 172, 244, 311, 393, 842, 898
Kzo9I GATC 4 cut(s) 52, 294, 1075, 1126
LmnI GCTCC 1 cut(s) 450
LweI GCATC 2 cut(s) 469, 661
MaeI CTAG 1 cut(s) 290
MaeIII GTNAC 3 cut(s) 177, 256, 485
MalI GATC 4 cut(s) 54, 296, 1077, 1128
MbiI CCGCTC 1 cut(s) 505
MboI GATC 4 cut(s) 52, 294, 1075, 1126
MfeI CAATTG 1 cut(s) 728
MflI RGATCY 1 cut(s) 1126
MhlI GDGCHC 1 cut(s) 240
MluCI AATT 4 cut(s) 118, 728, 780, 1045
MlyI GAGTC 2 cut(s) 512, 862
MmeI TCCRAC 1 cut(s) 844
MroXI GAANNNNTTC 1 cut(s) 320
MseI TTAA 3 cut(s) 90, 362, 1034
MslI CAYNNNNRTG 1 cut(s) 837
MspA1I CMGCKG 3 cut(s) 622, 1073, 1081
MspI CCGG 3 cut(s) 376, 454, 1065
MspR9I CCNGG 1 cut(s) 371
MunI CAATTG 1 cut(s) 728
MvaI CCWGG 1 cut(s) 371
NcoI CCATGG 1 cut(s) 389
NdeII GATC 4 cut(s) 52, 294, 1075, 1126
NlaIII CATG 6 cut(s) 172, 244, 311, 393, 842, 898
NlaIV GGNNCC 3 cut(s) 218, 452, 1128
NmeAIII GCCGAG 1 cut(s) 337
NmuCI GTSAC 2 cut(s) 177, 485
NspI RCATGY 2 cut(s) 244, 311
PciI ACATGT 1 cut(s) 240
PdmI GAANNNNTTC 1 cut(s) 320
PfeI GAWTC 2 cut(s) 332, 803
PflMI CCANNNNNTGG 1 cut(s) 447
PfoI TCCNGGA 1 cut(s) 369
PleI GAGTC 2 cut(s) 512, 861
PpsI GAGTC 2 cut(s) 512, 861
PscI ACATGT 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 369
PspN4I GGNNCC 3 cut(s) 218, 452, 1128
PspPI GGNCC 3 cut(s) 216, 373, 817
PsrI GAACNNNNNNTAC 2 cut(s) 884, 916
PstI CTGCAG 1 cut(s) 621
PsuI RGATCY 1 cut(s) 1126
PvuII CAGCTG 1 cut(s) 1081
RsaI GTAC 5 cut(s) 8, 101, 320, 533, 902
RsaNI GTAC 5 cut(s) 7, 100, 319, 532, 901
RseI CAYNNNNRTG 1 cut(s) 837
SaqAI TTAA 3 cut(s) 90, 362, 1034
Sau3AI GATC 4 cut(s) 52, 294, 1075, 1126
Sau96I GGNCC 3 cut(s) 216, 373, 817
ScaI AGTACT 3 cut(s) 8, 101, 320
SchI GAGTC 2 cut(s) 512, 862
ScrFI CCNGG 1 cut(s) 371
SduI GDGCHC 1 cut(s) 240
SetI ASST 8 cut(s) 109, 282, 404, 431, 477, 1002, 1027, 1083
SfaNI GCATC 2 cut(s) 469, 661
SfcI CTRYAG 1 cut(s) 617
SinI GGWCC 2 cut(s) 373, 817
SmiMI CAYNNNNRTG 1 cut(s) 837
Sse9I AATT 4 cut(s) 118, 728, 780, 1045
SspMI CTAG 1 cut(s) 290
StyD4I CCNGG 1 cut(s) 369
StyI CCWWGG 1 cut(s) 389
TaaI ACNGT 6 cut(s) 95, 227, 287, 419, 905, 955
TaqI TCGA 1 cut(s) 501
TasI AATT 4 cut(s) 118, 728, 780, 1045
TatI WGTACW 5 cut(s) 6, 99, 318, 531, 900
TauI GCSGC 4 cut(s) 552, 625, 652, 874
TfiI GAWTC 2 cut(s) 332, 803
Tru1I TTAA 3 cut(s) 90, 362, 1034
Tru9I TTAA 3 cut(s) 90, 362, 1034
TscAI CASTG 6 cut(s) 232, 651, 813, 819, 960, 1065
TseFI GTSAC 2 cut(s) 177, 485
Tsp45I GTSAC 2 cut(s) 177, 485
TspDTI ATGAA 4 cut(s) 157, 656, 731, 936
TspRI CASTG 6 cut(s) 232, 651, 813, 819, 960, 1065
Van91I CCANNNNNTGG 1 cut(s) 447
VpaK11BI GGWCC 2 cut(s) 373, 817
XceI RCATGY 2 cut(s) 244, 311
XcmI CCANNNNNNNNNTGG 2 cut(s) 166, 377
XmnI GAANNNNTTC 1 cut(s) 320
XspI CTAG 1 cut(s) 290
ZrmI AGTACT 3 cut(s) 8, 101, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.