Rmu_sc0002169.1_g000037

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002169.1
OG0012143
Physical Location & Seq
Reverse (-)
196716 .. 198083
1368 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1368 bp
atgggatggctccagatcttattgggagggcttgttataattgtcagcatatccagcctcttcaagttctactctgctggatttttcttgcgcaatgaagacatatgccgacatgtttatggggtcagagatgaatatgggggcttcgacataaaagctctgtctgatcgagtgggggaagtgctaaacaaattggatagtttacaagaaaagcttgagtcagctgtgcagcaaatggagaagaacaaagatgtcttgcatacaactaatatcacaaaactggagttcaagaagtatctggaggagcaagtaatcaggcctctttatagtgctcacatttctctgagactgattcggttacctaacccagaaggtattagaaactcaacaatgaaagaggaacctttgattaatacttttgtgattgaagagataaggaagtatataaccccaaaggagagtagaattgggaagattaatatatatggtacagagaggatatacaatacgattggacatgcttgtgtgttgtacaagaaagagttggaagagtacatgaactatgacatcgggtcttattgtaacgatgattggaacttggctcagaagctgatgtttaatggttgtgatcctttgccgcggaggcgatgtttgacaagggcctcaaaggtctatcagaagccttatccgatcaatgagtctctgtggaaattgccggatgacagaaatgtgcggtggagcaattatcagtgcaggaactttcagtgcttgatgagcaataattccaagaggggctattcaaagtgcgtaggatgttttgaaatggagaaggaaaagcttaaatggatgattaccaataaattgctaccagttgatttcctgatcagtgaaattttggctattaagcctggggagataagaattggtcttgactttggtgttggcacaggaacttatgctgcaagaatgagagaaaataatgttacggtagtctctactgctcttaaccttggggctcctttcaatgaaatgattgcattaagaggtttggttccgctttatgtaacattgaatcagcgtctacctttctttgataatataatggacttgattcataccgctgggtttctggatggctggattgacctgcagttgctggatttcatcctgtttgattgggaccgaattctgaggcctggcggattgttatggattgaccggttcttctgcaataaaaaggatttggatgatttcatgtatatgtttctgcagttgagatacaagaaacacaagtgggttatttctcccaaatcaaaggatgaggtctacttttctgcagtgttagagaaacctccaagagcaatctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

455

Amino Acids

53.13

Weight (kDa)

8.79

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 300 - 404 2.9e-06 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05390
fragaria_vesca FvH4_6g06250 FvH4_6g06250
malus_domestica MD12G1217000.v1.1
prunus_persica Prupe.6G323300_v2.0.a1
pyrus_communis pycom04g17960
rosa_chinensis RchiOBHm_Chr3g0455271
rosa_laevigata RLG00000025373
rosa_multiflora Rmu_sc0002169.1_g000037
rosa_roxburghii Rroxscaffold_6G00424410
rosa_rugosa Rorug03G0005500 Rorug03G0005600 Rorug03G0005700 Rorug03G0005800
rosa_samantha Rh3AG065600 Rh3BG067800 Rh3CG066200 Rh3DG067800
rosa_wichuraiana Rw3G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 38
Acc16I TGCGCA 1 cut(s) 92
Acc36I ACCTGC 1 cut(s) 1155
AccI GTMKAC 2 cut(s) 1081, 1326
AccII CGCG 1 cut(s) 640
AciI CCGC 6 cut(s) 638, 640, 733, 1055, 1119, 1200
AclWI GGATC 1 cut(s) 623
AcsI RAATTY 2 cut(s) 891, 1185
AfaI GTAC 3 cut(s) 490, 533, 554
AflIII ACRYGT 1 cut(s) 112
AgeI ACCGGT 1 cut(s) 1218
AgsI TTSAA 7 cut(s) 64, 289, 428, 801, 821, 1024, 1072
AhdI GACNNNNNGTC 1 cut(s) 571
AjnI CCWGG 2 cut(s) 907, 1195
AluBI AGCT 5 cut(s) 158, 214, 224, 610, 838
AluI AGCT 5 cut(s) 158, 214, 224, 610, 838
Alw21I GWGCWC 1 cut(s) 334
Alw26I GTCTC 3 cut(s) 340, 705, 997
AlwI GGATC 1 cut(s) 623
AlwNI CAGNNNCTG 2 cut(s) 610, 1156
AoxI GGCC 3 cut(s) 317, 660, 1193
ApeKI GCWGC 2 cut(s) 229, 959
ApoI RAATTY 2 cut(s) 891, 1185
AseI ATTAAT 2 cut(s) 411, 477
AsiGI ACCGGT 1 cut(s) 1218
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 2 cut(s) 660, 1180
AvaII GGWCC 1 cut(s) 1180
BanII GRGCYC 1 cut(s) 1018
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 334
BbvI GCAGC 2 cut(s) 241, 946
BccI CCATC 1 cut(s) 1127
BciT130I CCWGG 2 cut(s) 909, 1197
BclI TGATCA 1 cut(s) 882
BcoDI GTCTC 3 cut(s) 340, 705, 997
BfmI CTRYAG 3 cut(s) 1148, 1268, 1335
BfuAI ACCTGC 1 cut(s) 1155
BglI GCCNNNNNGGC 1 cut(s) 643
BglII AGATCT 1 cut(s) 15
BisI GCNGC 3 cut(s) 230, 638, 960
BlsI GCNGC 3 cut(s) 231, 639, 961
Bme1390I CCNGG 2 cut(s) 909, 1197
Bme18I GGWCC 1 cut(s) 1180
BmeRI GACNNNNNGTC 1 cut(s) 571
BmgT120I GGNCC 2 cut(s) 660, 1180
BmiI GGNNCC 5 cut(s) 11, 402, 1017, 1053, 1181
BmrFI CCNGG 2 cut(s) 909, 1197
BpiI GAAGAC 1 cut(s) 105
BpmI CTGGAG 2 cut(s) 302, 320
BpuEI CTTGAG 1 cut(s) 236
BsaJI CCNNGG 3 cut(s) 638, 908, 1009
BsaWI WCCGGW 1 cut(s) 1218
BsaXI ACNNNNNCTCC 4 cut(s) 18, 48, 634, 664
Bse118I RCCGGY 1 cut(s) 1218
Bse1I ACTGG 2 cut(s) 285, 869
Bse3DI GCAATG 1 cut(s) 100
BseBI CCWGG 2 cut(s) 909, 1197
BseDI CCNNGG 3 cut(s) 638, 908, 1009
BseGI GGATG 8 cut(s) 11, 724, 818, 852, 1138, 1164, 1252, 1324
BseMI GCAATG 1 cut(s) 100
BseMII CTCAG 3 cut(s) 335, 617, 1181
BseNI ACTGG 2 cut(s) 285, 869
BseRI GAGGAG 1 cut(s) 317
BseXI GCAGC 2 cut(s) 241, 946
BseYI CCCAGC 1 cut(s) 1121
BsgI GTGCAG 2 cut(s) 248, 772
Bsh1236I CGCG 1 cut(s) 640
BshFI GGCC 3 cut(s) 319, 662, 1195
BshTI ACCGGT 1 cut(s) 1218
BsiHKAI GWGCWC 1 cut(s) 334
BsiSI CCGG 2 cut(s) 716, 1219
BslFI GGGAC 1 cut(s) 1193
BsmAI GTCTC 3 cut(s) 340, 705, 997
BsmFI GGGAC 1 cut(s) 1193
BsnI GGCC 3 cut(s) 319, 662, 1195
Bsp1286I GDGCHC 2 cut(s) 334, 1018
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 5 cut(s) 15, 166, 628, 690, 882
BspACI CCGC 6 cut(s) 638, 640, 733, 1055, 1119, 1200
BspANI GGCC 3 cut(s) 319, 662, 1195
BspCNI CTCAG 3 cut(s) 336, 616, 1182
BspFNI CGCG 1 cut(s) 640
BspLI GGNNCC 5 cut(s) 11, 402, 1017, 1053, 1181
BspMAI CTGCAG 3 cut(s) 1152, 1272, 1339
BspMI ACCTGC 1 cut(s) 1155
BspPI GGATC 1 cut(s) 623
BsrDI GCAATG 1 cut(s) 100
BsrFI RCCGGY 1 cut(s) 1218
BsrGI TGTACA 1 cut(s) 531
BsrI ACTGG 2 cut(s) 285, 869
BssAI RCCGGY 1 cut(s) 1218
BssECI CCNNGG 3 cut(s) 638, 908, 1009
BssMI GATC 5 cut(s) 15, 166, 628, 690, 882
BssT1I CCWWGG 1 cut(s) 1009
Bst2UI CCWGG 2 cut(s) 909, 1197
Bst4CI ACNGT 1 cut(s) 988
Bst6I CTCTTC 3 cut(s) 65, 423, 543
BstAUI TGTACA 1 cut(s) 531
BstDEI CTNAG 3 cut(s) 344, 603, 1190
BstDSI CCRYGG 1 cut(s) 638
BstEII GGTNACC 1 cut(s) 357
BstF5I GGATG 8 cut(s) 11, 724, 818, 852, 1138, 1164, 1252, 1324
BstFNI CGCG 1 cut(s) 640
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 5 cut(s) 18, 169, 631, 693, 885
BstMAI GTCTC 3 cut(s) 340, 705, 997
BstMBI GATC 5 cut(s) 15, 166, 628, 690, 882
BstMWI GCNNNNNNNGC 3 cut(s) 54, 643, 774
BstNI CCWGG 2 cut(s) 909, 1197
BstNSI RCATGY 2 cut(s) 116, 521
BstPI GGTNACC 1 cut(s) 357
BstSCI CCNGG 2 cut(s) 907, 1195
BstSFI CTRYAG 3 cut(s) 1148, 1268, 1335
BstUI CGCG 1 cut(s) 640
BstV1I GCAGC 2 cut(s) 241, 946
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 15
BstYI RGATCY 1 cut(s) 15
BsuRI GGCC 3 cut(s) 319, 662, 1195
BtgI CCRYGG 1 cut(s) 638
BtgZI GCGATG 1 cut(s) 661
BtsCI GGATG 8 cut(s) 11, 724, 818, 852, 1138, 1164, 1252, 1324
BtsI GCAGTG 1 cut(s) 1344
BtsIMutI CAGTG 4 cut(s) 755, 770, 892, 1344
BveI ACCTGC 1 cut(s) 1155
CaiI CAGNNNCTG 2 cut(s) 610, 1156
CfoI GCGC 1 cut(s) 93
Cfr10I RCCGGY 1 cut(s) 1218
Cfr13I GGNCC 2 cut(s) 660, 1180
Cfr42I CCGCGG 1 cut(s) 641
CseI GACGC 1 cut(s) 1067
Csp6I GTAC 3 cut(s) 489, 532, 553
CspAI ACCGGT 1 cut(s) 1218
CviAII CATG 4 cut(s) 113, 518, 556, 1255
CviQI GTAC 3 cut(s) 489, 532, 553
DdeI CTNAG 3 cut(s) 344, 603, 1190
DpnI GATC 5 cut(s) 17, 168, 630, 692, 884
DpnII GATC 5 cut(s) 15, 166, 628, 690, 882
DriI GACNNNNNGTC 1 cut(s) 571
Eam1104I CTCTTC 3 cut(s) 65, 423, 543
Eam1105I GACNNNNNGTC 1 cut(s) 571
EarI CTCTTC 3 cut(s) 65, 423, 543
EciI GGCGGA 1 cut(s) 1215
Eco130I CCWWGG 1 cut(s) 1009
Eco147I AGGCCT 2 cut(s) 319, 1195
Eco24I GRGCYC 1 cut(s) 1018
Eco47I GGWCC 1 cut(s) 1180
Eco91I GGTNACC 1 cut(s) 357
EcoO109I RGGNCCY 1 cut(s) 660
EcoO65I GGTNACC 1 cut(s) 357
EcoRI GAATTC 1 cut(s) 1185
EcoRII CCWGG 2 cut(s) 907, 1195
EcoT14I CCWWGG 1 cut(s) 1009
EcoT38I GRGCYC 1 cut(s) 1018
ErhI CCWWGG 1 cut(s) 1009
FaeI CATG 4 cut(s) 116, 521, 559, 1258
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FaqI GGGAC 1 cut(s) 1193
FatI CATG 4 cut(s) 112, 517, 555, 1254
FauNDI CATATG 1 cut(s) 104
FbaI TGATCA 1 cut(s) 882
FblI GTMKAC 2 cut(s) 1081, 1326
Fnu4HI GCNGC 3 cut(s) 230, 638, 960
FokI GGATG 8 cut(s) 18, 731, 825, 859, 1145, 1151, 1259, 1331
FriOI GRGCYC 1 cut(s) 1018
Fsp4HI GCNGC 3 cut(s) 230, 638, 960
FspI TGCGCA 1 cut(s) 92
GlaI GCGC 1 cut(s) 92
GluI GCNGC 3 cut(s) 230, 638, 960
GsaI CCCAGC 1 cut(s) 1125
GsuI CTGGAG 2 cut(s) 302, 320
HaeIII GGCC 3 cut(s) 319, 662, 1195
HapII CCGG 2 cut(s) 716, 1219
HgaI GACGC 1 cut(s) 1067
HhaI GCGC 1 cut(s) 93
Hin1II CATG 4 cut(s) 116, 521, 559, 1258
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HindIII AAGCTT 2 cut(s) 212, 836
HinfI GANTC 5 cut(s) 218, 352, 698, 1072, 1111
HpaII CCGG 2 cut(s) 716, 1219
Hpy166II GTNNAC 3 cut(s) 203, 1082, 1327
Hpy188I TCNGA 8 cut(s) 128, 166, 345, 606, 678, 690, 1191, 1367
Hpy188III TCNNGA 6 cut(s) 13, 289, 299, 880, 929, 1130
Hpy8I GTNNAC 3 cut(s) 203, 1082, 1327
HpyAV CCTTC 2 cut(s) 365, 823
HpyCH4III ACNGT 1 cut(s) 988
HpyCH4V TGCA 9 cut(s) 229, 259, 753, 962, 1037, 1150, 1230, 1270, 1337
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 643, 774
HpyF3I CTNAG 3 cut(s) 344, 603, 1190
Hsp92II CATG 4 cut(s) 116, 521, 559, 1258
HspAI GCGC 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 882
KspI CCGCGG 1 cut(s) 641
Kzo9I GATC 5 cut(s) 15, 166, 628, 690, 882
LmnI GCTCC 4 cut(s) 15, 304, 738, 1021
Lsp1109I GCAGC 2 cut(s) 241, 946
MaeIII GTNAC 4 cut(s) 357, 581, 982, 1063
MalI GATC 5 cut(s) 17, 168, 630, 692, 884
MboI GATC 5 cut(s) 15, 166, 628, 690, 882
MboII GAAGA 7 cut(s) 52, 110, 253, 440, 484, 560, 1216
MflI RGATCY 1 cut(s) 15
MhlI GDGCHC 2 cut(s) 334, 1018
MlyI GAGTC 2 cut(s) 227, 707
MmeI TCCRAC 1 cut(s) 525
MseI TTAA 7 cut(s) 411, 477, 618, 840, 903, 1005, 1040
MslI CAYNNNNRTG 3 cut(s) 117, 522, 1259
MspA1I CMGCKG 3 cut(s) 224, 640, 1121
MspI CCGG 2 cut(s) 716, 1219
MspR9I CCNGG 2 cut(s) 909, 1197
MvaI CCWGG 2 cut(s) 909, 1197
MvnI CGCG 1 cut(s) 640
MwoI GCNNNNNNNGC 3 cut(s) 54, 643, 774
NdeI CATATG 1 cut(s) 104
NdeII GATC 5 cut(s) 15, 166, 628, 690, 882
NlaIII CATG 4 cut(s) 116, 521, 559, 1258
NlaIV GGNNCC 5 cut(s) 11, 402, 1017, 1053, 1181
NsbI TGCGCA 1 cut(s) 92
NspI RCATGY 2 cut(s) 116, 521
PceI AGGCCT 2 cut(s) 319, 1195
PciI ACATGT 1 cut(s) 112
PfeI GAWTC 3 cut(s) 352, 1072, 1111
PinAI ACCGGT 1 cut(s) 1218
PkrI GCNGC 3 cut(s) 231, 639, 961
PleI GAGTC 2 cut(s) 226, 706
PpsI GAGTC 2 cut(s) 226, 706
PscI ACATGT 1 cut(s) 112
PshBI ATTAAT 2 cut(s) 411, 477
PsiI TTATAA 1 cut(s) 38
Psp6I CCWGG 2 cut(s) 907, 1195
PspEI GGTNACC 1 cut(s) 357
PspFI CCCAGC 1 cut(s) 1121
PspGI CCWGG 2 cut(s) 907, 1195
PspN4I GGNNCC 5 cut(s) 11, 402, 1017, 1053, 1181
PspPI GGNCC 2 cut(s) 660, 1180
PstI CTGCAG 3 cut(s) 1152, 1272, 1339
PstNI CAGNNNCTG 2 cut(s) 610, 1156
PsuI RGATCY 1 cut(s) 15
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 3 cut(s) 490, 533, 554
RsaNI GTAC 3 cut(s) 489, 532, 553
RseI CAYNNNNRTG 3 cut(s) 117, 522, 1259
SacII CCGCGG 1 cut(s) 641
SaqAI TTAA 7 cut(s) 411, 477, 618, 840, 903, 1005, 1040
SatI GCNGC 3 cut(s) 230, 638, 960
Sau3AI GATC 5 cut(s) 15, 166, 628, 690, 882
Sau96I GGNCC 2 cut(s) 660, 1180
SchI GAGTC 2 cut(s) 227, 707
ScrFI CCNGG 2 cut(s) 909, 1197
SduI GDGCHC 2 cut(s) 334, 1018
SfcI CTRYAG 3 cut(s) 1148, 1268, 1335
Sfr303I CCGCGG 1 cut(s) 641
SgrBI CCGCGG 1 cut(s) 641
SinI GGWCC 1 cut(s) 1180
SmiMI CAYNNNNRTG 3 cut(s) 117, 522, 1259
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SseBI AGGCCT 2 cut(s) 319, 1195
SsiI CCGC 6 cut(s) 638, 640, 733, 1055, 1119, 1200
StuI AGGCCT 2 cut(s) 319, 1195
StyD4I CCNGG 2 cut(s) 907, 1195
StyI CCWWGG 1 cut(s) 1009
TaaI ACNGT 1 cut(s) 988
TaqI TCGA 2 cut(s) 147, 169
TaqII GACCGA 1 cut(s) 1197
TatI WGTACW 2 cut(s) 531, 552
TauI GCSGC 1 cut(s) 640
TfiI GAWTC 3 cut(s) 352, 1072, 1111
Tru1I TTAA 7 cut(s) 411, 477, 618, 840, 903, 1005, 1040
Tru9I TTAA 7 cut(s) 411, 477, 618, 840, 903, 1005, 1040
TscAI CASTG 4 cut(s) 755, 770, 892, 1344
TseI GCWGC 2 cut(s) 229, 959
TspDTI ATGAA 8 cut(s) 111, 147, 407, 572, 1041, 1103, 1153, 1243
TspRI CASTG 4 cut(s) 755, 770, 892, 1344
VpaK11BI GGWCC 1 cut(s) 1180
VspI ATTAAT 2 cut(s) 411, 477
XapI RAATTY 2 cut(s) 891, 1185
XceI RCATGY 2 cut(s) 116, 521
XmiI GTMKAC 2 cut(s) 1081, 1326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.