Rmu_sc0002226.1_g000002

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002226.1
OG0016760
Physical Location & Seq
Forward (+)
8296 .. 15064
6769 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4179 bp
atggactcgattttaaactcatccttgcaagaaatctgctcggaaggtccaaacggtctccctcttcaaaccctctcctccaggctcaacctctctccgccactccaacaacccctatgggcggcgctactctccgtccccgccctcaaattccacgcccaaacccaaaacgccaccgtttcgcacctccccacagaccgttcaatccagtcgttccgcgacgccgagaagctcaacctgaagctcgtcgccgaccagcctctccgcaacaacttcctcggcgaacaacccctatgggcggcgctactctccgtccccgccctcaaattccacgcccaaacccaaaacgccaccgtttcgcacctccccacagacccttcaatccagtcgttccgcgacgccgagaagctcaacctgaagctcgtcgccgaccagcctctccgcaacaacttcctcggcctctacgacgtccagtccgcatccgacaccatgtgcgagtaccaacgcaaaaccctagagcgcgtggccgcggctgggtcaaaggggatcacgcagagtcaactatccaaggagcttgggattagaggtgagaatttcttttacaaattgaggaggctcgaatgtcagggcttgattgtgaggcaaccattgaatgatgatgccaatgtgagtgggagtactaatttagtgcacttaaatcgttatgcaaagcatttaggtctagggtctgaacaaaaagctgaggtcactaaatctaaggaggaagagaatatgactaagaagagtgggtctgaacaaaaagctgaggtcactaaatctaaggaggaagagaatatgactaagaagagtggtaatttgatagcggaacttccgattgagcatcaaatttacgagctagttaatgatgcggggtcaaggggggtcgtggtgtttaaggttgcagagaggctgggaattgataggaaagagaataggaagaggattgaaagtttgtgctccaagtttgggatgataatggataaagagaaatgcggaaagactaaagtgtatcgagtttgggcacctgggaaacgtaatgctgaatcagctaatgcaattctgaatgagtctgaaaatgttactcaaagtaaatttaatgaagatgttcttcgtgggtccgttcagaaaggtgatacagcaataaataatgtctcatcaggaatgccacctgctgtggaatgtgctaggcccagaaggggaaaaagtgtacttgaaccatcacaggataaaggggatgaacgtcaatctctgggaattagaagccctttgcagaaaagaaagagatcttctgaagcagcttctgtaaagtttatagaagttgatggtgtaactgagcaccaacctggaccaaatgaagatggtgaggcgaggtgtgctttttcaaagtcaaaatcaacatgtcaaagaaggtttttatggacagaggaggctgataggcaattagttatccaatatgtaagacggtgtgcaactcttggggcaaaatatcatcaccagataaattgggcttcactttcggaccttccagctcctccaagtacctgcaaaagaagaatgtcatatctgaagctcagtaatggaaaatttagaaaatctttaatgacactgtgtaacatgctcagtaaacggtatgcaaagctcctgcagaaaacccagaacaggccattttaccaagatgattgtggatcacttttgcaaggttcaacagaggagggttgtaaacagaattatcctctcagtgaccatgggacaggtcttcaggaagaacaatgggatgactttaatgacaaaaatattaaagaagtgcttgatcagatacttcactatgtgaggttgactaaatctgttgcctccaaaagatgtggagcttcctgcgaggagggttcagaacttaatgctaatgctgaagatgttggaggcagtgattgtgaacctttgtctctctcacctcaggtcttccccaatatttgcaagtcaccatttccaactgattccggcaagagagcagccaaatttgctcattggctccacaaaagagatagagatcttatgggaagggggcttgatcttcctacagatatacagtgtggtgaattttttcatatgtttgctctagttttttcaggtgaattgtcaatctccccatgcttgccagatggagtgagagaagcagaagatttgagaagctcaaaacataaaattggtaggaataaacattcggatgctaaaagactaaaatccttggttgcagaaagtgagattatttcccgtcgagaaaaaggttttccgggtctcatggtatctatatgtcgtacagcatcttctaaagcctattgtgaaaatttgttcaaagatgatgatactctaggtggtgacaaacattttggtgaaaattatcagcaagacagtacttcaggtgtgaatcagatgagagaaaacctcaattcaggcattgttatccctgtggcagagacttgtagtgagtcacctggcggaggtatcgtccatcctggaaattgtgatattattggaactcttttgatagggaaaacaaatatgaatgttaatgatgcacataatgtgacttttcttaattctcctaaagaggttgatgagcgtccgtctgaaaaactaactagcggtgtggaaggcaaagttatctcacctagaggagattttgatgaatgttctaaactctcttctgatgaattatgcgtgccacttcttccttggataaatggggacgagaccatcaacaagactatccacaaggggttcatgcaacgtgtttttgggattgtgatgcaaaaccccgggatattagagggtgaaattatccgtaagatggatgtactgaatccgcagagttgtagaaaattgttggtggagatgattgaagcaaaacatcttcatgtcaggacaatgcgccaggccacattgaatggccccccagcatttctagggaacctatttggaagcagcctcacccagtcaaaggtggttggaggcagtgattgtgaacctttgtctctctcacctcaggtcttccccaatatttgcaagtcaccatttccaactgattccggcaagagagcagccaaatttgctcattggctccacaaaagagatagagatcttatgggaagggggcttgatcttcctacagatatacagtgtggtgaattttttcatatgtttgctctagttttttcaggtgaattgtcaatctccccatgcttgccagatggagtgagagaagcagaagatttgagaagctcaaaacataaaattggtaggaataaacattcggatgctaaaagactaaaatccttggttgcagaaagtgagattatttcccgtcgagaaaaaggtttcccgggtctcatggtatctatatgtcgtacagcatcttctaaagcctattgtgaaaatttgttcaaagatgatgatactctaggtggtgacaaacattttggtgaaaattatcagcaagacagtacttcaggtgtgaatcagatgagagaaaacctcaattcaggcattgttatccctgtggcagagacttgtagtgagtcacctggcggaggtatcctccatcctggaaattgtgatattattggaactcttttgatagggaaaacaaatatgaatgttaatgatgcacataatgtgacttttcttaattctcctaaagaggttgatgagcgtccgtctgaaaaactaactagcggtgtggaaggcaaagttatctcacctagaggagattttgatgaatgttctaaactctcttctgatgaattatgcgtgccacttcctccttggataaatggggacgagaccatcaacaagactatccacaaggggttcatccaacgtgtttttgggattgtgatgcaaaaccccgggatattagagggtgaaattatccgtaagatggatgtactgaatccgcagagttgtagaaaattgttggtggagatgattgaagcaaaacatcttcatgtcaggacaatgcgccaagccacatggaatggccccccagcatttctagggaacctatttggaagcagcctcacccagtcaaaggtggttttccgtaggcatctctttgcaaattccatgagcgcgttcttgctatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B-block_TFIIIC

Protein Analysis

1392

Amino Acids

154.3

Weight (kDa)

8.29

Isoelectric Point (pI)

48.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_GTF3C1_N PF23704 1 - 94 1.3e-22 General transcription factor 3C polypeptide 1, winged-helix domain
WHD_GTF3C1_N PF23704 96 - 155 1e-12 General transcription factor 3C polypeptide 1, winged-helix domain
B-block_TFIIIC PF04182 167 - 237 7.1e-14 B-block binding subunit of TFIIIC
DUF7646 PF24657 285 - 361 1.2e-27 Domain of unknown function (DUF7646)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016760)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g23920 FvH4_2g23920
malus_domestica MD11G1308600.v1.1
prunus_persica Prupe.8G262800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0291551
rosa_laevigata RLG00000012108
rosa_multiflora Rmu_sc0002226.1_g000002
rosa_roxburghii Rroxscaffold_7G00175980
rosa_rugosa Rorug06G0220200
rosa_samantha Rh6AG332400 Rh6BG339300 Rh6CG345600 Rh6DG332900
rosa_wichuraiana Rw6G028870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1216
AatII GACGTC 1 cut(s) 471
Acc36I ACCTGC 2 cut(s) 1216, 1589
AccB1I GGYRCC 1 cut(s) 1060
AccII CGCG 5 cut(s) 219, 396, 522, 530, 4166
AclWI GGATC 2 cut(s) 554, 1732
AcoI YGGCCR 1 cut(s) 525
AcuI CTGAAG 8 cut(s) 260, 437, 1350, 1625, 1781, 1965, 2436, 3525
AcyI GRCGYC 3 cut(s) 222, 399, 468
AdeI CACNNNGTG 2 cut(s) 492, 1867
AflIII ACRYGT 3 cut(s) 1436, 2824, 3913
AhdI GACNNNNNGTC 1 cut(s) 472
AjnI CCWGG 8 cut(s) 80, 1063, 1381, 2527, 2548, 2967, 3616, 3637
Alw21I GWGCWC 3 cut(s) 693, 998, 1377
Alw44I GTGCAC 1 cut(s) 689
AlwI GGATC 2 cut(s) 554, 1732
AlwNI CAGNNNCTG 1 cut(s) 1340
Ama87I CYCGRG 3 cut(s) 2852, 3415, 3941
AoxI GGCC 7 cut(s) 457, 525, 1226, 1700, 2970, 2983, 4072
ApaLI GTGCAC 1 cut(s) 689
ApeKI GCWGC 5 cut(s) 1334, 2045, 3018, 3134, 4107
ArsI GACNNNNNNTTYG 4 cut(s) 2405, 2437, 3494, 3526
Asp700I GAANNNNTTC 3 cut(s) 1143, 2136, 3225
AspLEI GCGC 6 cut(s) 127, 304, 522, 2967, 4056, 4166
AspS9I GGNCC 7 cut(s) 47, 1155, 1227, 1385, 1558, 2984, 4073
AsuC2I CCSGG 7 cut(s) 2328, 2853, 2854, 3416, 3417, 3942, 3943
AvaI CYCGRG 3 cut(s) 2852, 3415, 3941
AvaII GGWCC 4 cut(s) 47, 1155, 1385, 1558
AxyI CCTNAGG 2 cut(s) 1989, 3078
BaeGI GKGCMC 2 cut(s) 693, 1063
BanI GGYRCC 1 cut(s) 1060
BarI GAAGNNNNNNTAC 4 cut(s) 2344, 2376, 3433, 3465
BbsI GAAGAC 3 cut(s) 1787, 1987, 3076
Bbv12I GWGCWC 3 cut(s) 693, 998, 1377
BbvCI CCTCAGC 2 cut(s) 741, 804
BbvI GCAGC 5 cut(s) 1346, 2057, 3030, 3146, 4119
BcgI CGANNNNNNTGC 2 cut(s) 680, 714
BciT130I CCWGG 8 cut(s) 82, 1065, 1383, 2529, 2550, 2969, 3618, 3639
BciVI GTATCC 1 cut(s) 3638
BclI TGATCA 1 cut(s) 1849
BcnI CCSGG 7 cut(s) 2328, 2853, 2854, 3416, 3417, 3942, 3943
BfmI CTRYAG 4 cut(s) 1682, 2112, 3201, 4175
BfoI RGCGCY 2 cut(s) 128, 305
BfuAI ACCTGC 2 cut(s) 1216, 1589
BfuI GTATCC 1 cut(s) 3638
BglII AGATCT 3 cut(s) 1322, 2083, 3172
BisI GCNGC 9 cut(s) 123, 300, 528, 531, 1335, 2046, 3019, 3135, 4108
BlsI GCNGC 9 cut(s) 124, 301, 529, 532, 1336, 2047, 3020, 3136, 4109
BmcAI AGTACT 3 cut(s) 679, 2449, 3538
Bme18I GGWCC 4 cut(s) 47, 1155, 1385, 1558
BmeRI GACNNNNNGTC 1 cut(s) 472
BmeT110I CYCGRG 3 cut(s) 2852, 3415, 3941
BmgT120I GGNCC 7 cut(s) 47, 1155, 1227, 1385, 1558, 2984, 4073
BmiI GGNNCC 8 cut(s) 1062, 1156, 2066, 2986, 3005, 3155, 4075, 4094
BmrI ACTGGG 2 cut(s) 3022, 4111
BmuI ACTGGG 2 cut(s) 3022, 4111
BpiI GAAGAC 3 cut(s) 1787, 1987, 3076
BplI GAGNNNNNCTC 6 cut(s) 2463, 2495, 3552, 3584, 3615, 3647
BpmI CTGGAG 1 cut(s) 64
Bpu10I CCTNAGC 2 cut(s) 741, 804
BpuMI CCSGG 7 cut(s) 2328, 2853, 2854, 3416, 3417, 3942, 3943
BsaBI GATNNNNATC 2 cut(s) 2082, 3171
BsaHI GRCGYC 3 cut(s) 222, 399, 468
BsaI GGTCTC 5 cut(s) 62, 2336, 2780, 3425, 3869
BsaXI ACNNNNNCTCC 2 cut(s) 2526, 2556
Bse1I ACTGG 5 cut(s) 208, 385, 472, 3028, 4117
Bse21I CCTNAGG 2 cut(s) 1989, 3078
Bse8I GATNNNNATC 2 cut(s) 2082, 3171
BseBI CCWGG 8 cut(s) 82, 1065, 1383, 2529, 2550, 2969, 3618, 3639
BseJI GATNNNNATC 2 cut(s) 2082, 3171
BseMII CTCAG 8 cut(s) 732, 795, 1362, 1624, 1672, 1789, 2003, 3092
BseNI ACTGG 5 cut(s) 208, 385, 472, 3028, 4117
BseRI GAGGAG 8 cut(s) 67, 625, 1478, 1560, 1763, 1931, 2724, 3813
BseSI GKGCMC 2 cut(s) 693, 1063
BseXI GCAGC 5 cut(s) 1346, 2057, 3030, 3146, 4119
BseYI CCCAGC 4 cut(s) 533, 949, 2989, 4078
Bsh1236I CGCG 5 cut(s) 219, 396, 522, 530, 4166
BshFI GGCC 7 cut(s) 459, 527, 1228, 1702, 2972, 2985, 4074
BshNI GGYRCC 1 cut(s) 1060
BsiHKAI GWGCWC 3 cut(s) 693, 998, 1377
BsiHKCI CYCGRG 3 cut(s) 2852, 3415, 3941
BsiSI CCGG 6 cut(s) 2034, 2327, 2853, 3123, 3416, 3942
BslFI GGGAC 5 cut(s) 122, 299, 1801, 2795, 3884
BsmFI GGGAC 5 cut(s) 122, 299, 1801, 2795, 3884
BsmI GAATGC 1 cut(s) 1206
BsnI GGCC 7 cut(s) 459, 527, 1228, 1702, 2972, 2985, 4074
Bso31I GGTCTC 5 cut(s) 62, 2336, 2780, 3425, 3869
BsoBI CYCGRG 3 cut(s) 2852, 3415, 3941
Bsp1286I GDGCHC 4 cut(s) 693, 998, 1063, 1377
Bsp143I GATC 8 cut(s) 546, 1322, 1724, 1849, 2083, 2104, 3172, 3193
Bsp19I CCATGG 1 cut(s) 1783
BspANI GGCC 7 cut(s) 459, 527, 1228, 1702, 2972, 2985, 4074
BspCNI CTCAG 8 cut(s) 733, 796, 1363, 1623, 1671, 1788, 2002, 3091
BspFNI CGCG 5 cut(s) 219, 396, 522, 530, 4166
BspLI GGNNCC 8 cut(s) 1062, 1156, 2066, 2986, 3005, 3155, 4075, 4094
BspMAI CTGCAG 1 cut(s) 1686
BspMI ACCTGC 2 cut(s) 1216, 1589
BspPI GGATC 2 cut(s) 554, 1732
BspT107I GGYRCC 1 cut(s) 1060
BspTNI GGTCTC 5 cut(s) 62, 2336, 2780, 3425, 3869
BsrI ACTGG 5 cut(s) 208, 385, 472, 3028, 4117
BssMI GATC 8 cut(s) 546, 1322, 1724, 1849, 2083, 2104, 3172, 3193
BssNI GRCGYC 3 cut(s) 222, 399, 468
BssT1I CCWWGG 6 cut(s) 567, 1783, 2280, 2768, 3369, 3857
Bst2UI CCWGG 8 cut(s) 82, 1065, 1383, 2529, 2550, 2969, 3618, 3639
Bst6I CTCTTC 8 cut(s) 69, 759, 776, 822, 839, 971, 2743, 3832
BstACI GRCGYC 3 cut(s) 222, 399, 468
BstC8I GCNNGC 4 cut(s) 2189, 2756, 3278, 3845
BstDSI CCRYGG 2 cut(s) 528, 1783
BstENI CCTNNNNNAGG 2 cut(s) 2532, 3621
BstFNI CGCG 5 cut(s) 219, 396, 522, 530, 4166
BstH2I RGCGCY 2 cut(s) 128, 305
BstHHI GCGC 6 cut(s) 127, 304, 522, 2967, 4056, 4166
BstKTI GATC 8 cut(s) 549, 1325, 1727, 1852, 2086, 2107, 3175, 3196
BstMBI GATC 8 cut(s) 546, 1322, 1724, 1849, 2083, 2104, 3172, 3193
BstMWI GCNNNNNNNGC 4 cut(s) 1085, 1412, 2054, 3143
BstNI CCWGG 8 cut(s) 82, 1065, 1383, 2529, 2550, 2969, 3618, 3639
BstNSI RCATGY 2 cut(s) 1440, 1657
BstSFI CTRYAG 4 cut(s) 1682, 2112, 3201, 4175
BstSLI GKGCMC 2 cut(s) 693, 1063
BstUI CGCG 5 cut(s) 219, 396, 522, 530, 4166
BstV1I GCAGC 5 cut(s) 1346, 2057, 3030, 3146, 4119
BstV2I GAAGAC 3 cut(s) 1787, 1987, 3076
BstX2I RGATCY 3 cut(s) 1322, 2083, 3172
BstYI RGATCY 3 cut(s) 1322, 2083, 3172
Bsu36I CCTNAGG 2 cut(s) 1989, 3078
BsuI GTATCC 1 cut(s) 3638
BsuRI GGCC 7 cut(s) 459, 527, 1228, 1702, 2972, 2985, 4074
BtgI CCRYGG 2 cut(s) 528, 1783
BtsI GCAGTG 2 cut(s) 1966, 3055
BtsIMutI CAGTG 6 cut(s) 1643, 1783, 1966, 2129, 3055, 3218
BveI ACCTGC 2 cut(s) 1216, 1589
Cac8I GCNNGC 4 cut(s) 2189, 2756, 3278, 3845
CaiI CAGNNNCTG 1 cut(s) 1340
CfoI GCGC 6 cut(s) 127, 304, 522, 2967, 4056, 4166
Cfr13I GGNCC 7 cut(s) 47, 1155, 1227, 1385, 1558, 2984, 4073
Cfr42I CCGCGG 1 cut(s) 531
Cfr9I CCCGGG 3 cut(s) 2852, 3415, 3941
CseI GACGC 4 cut(s) 230, 407, 2645, 3734
CspCI CAANNNNNGTGG 4 cut(s) 652, 687, 1882, 1917
DpnI GATC 8 cut(s) 548, 1324, 1726, 1851, 2085, 2106, 3174, 3195
DpnII GATC 8 cut(s) 546, 1322, 1724, 1849, 2083, 2104, 3172, 3193
DraI TTTAAA 1 cut(s) 15
DraIII CACNNNGTG 2 cut(s) 492, 1867
DriI GACNNNNNGTC 1 cut(s) 472
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 8 cut(s) 69, 759, 776, 822, 839, 971, 2743, 3832
Eam1105I GACNNNNNGTC 1 cut(s) 472
EarI CTCTTC 8 cut(s) 69, 759, 776, 822, 839, 971, 2743, 3832
EciI GGCGGA 3 cut(s) 87, 2547, 3636
Eco130I CCWWGG 6 cut(s) 567, 1783, 2280, 2768, 3369, 3857
Eco31I GGTCTC 5 cut(s) 62, 2336, 2780, 3425, 3869
Eco47I GGWCC 4 cut(s) 47, 1155, 1385, 1558
Eco57I CTGAAG 8 cut(s) 260, 437, 1350, 1625, 1781, 1965, 2436, 3525
Eco81I CCTNAGG 2 cut(s) 1989, 3078
Eco88I CYCGRG 3 cut(s) 2852, 3415, 3941
EcoNI CCTNNNNNAGG 2 cut(s) 2532, 3621
EcoRII CCWGG 8 cut(s) 80, 1063, 1381, 2527, 2548, 2967, 3616, 3637
EcoT14I CCWWGG 6 cut(s) 567, 1783, 2280, 2768, 3369, 3857
ErhI CCWWGG 6 cut(s) 567, 1783, 2280, 2768, 3369, 3857
FalI AAGNNNNNCTT 4 cut(s) 1131, 1163, 1830, 1862
FaqI GGGAC 5 cut(s) 122, 299, 1801, 2795, 3884
FauI CCCGC 3 cut(s) 148, 325, 901
FauNDI CATATG 2 cut(s) 2142, 3231
FbaI TGATCA 1 cut(s) 1849
Fnu4HI GCNGC 9 cut(s) 123, 300, 528, 531, 1335, 2046, 3019, 3135, 4108
Fsp4HI GCNGC 9 cut(s) 123, 300, 528, 531, 1335, 2046, 3019, 3135, 4108
GlaI GCGC 6 cut(s) 126, 303, 521, 2966, 4055, 4165
GluI GCNGC 9 cut(s) 123, 300, 528, 531, 1335, 2046, 3019, 3135, 4108
GsaI CCCAGC 4 cut(s) 537, 953, 2993, 4082
GsuI CTGGAG 1 cut(s) 64
HaeII RGCGCY 2 cut(s) 128, 305
HaeIII GGCC 7 cut(s) 459, 527, 1228, 1702, 2972, 2985, 4074
HapII CCGG 6 cut(s) 2034, 2327, 2853, 3123, 3416, 3942
HgaI GACGC 4 cut(s) 230, 407, 2645, 3734
HhaI GCGC 6 cut(s) 127, 304, 522, 2967, 4056, 4166
Hin1I GRCGYC 3 cut(s) 222, 399, 468
Hin6I GCGC 6 cut(s) 125, 302, 520, 2965, 4054, 4164
HinP1I GCGC 6 cut(s) 125, 302, 520, 2965, 4054, 4164
HincII GTYRAC 2 cut(s) 560, 1875
HindII GTYRAC 2 cut(s) 560, 1875
HpaII CCGG 6 cut(s) 2034, 2327, 2853, 3123, 3416, 3942
Hpy166II GTNNAC 8 cut(s) 560, 691, 1247, 1664, 1760, 1875, 1970, 3059
Hpy188III TCNNGA 6 cut(s) 1197, 1799, 2312, 2956, 3401, 4045
Hpy8I GTNNAC 8 cut(s) 560, 691, 1247, 1664, 1760, 1875, 1970, 3059
Hpy99I CGWCG 7 cut(s) 224, 251, 401, 428, 470, 2313, 3402
HpyAV CCTTC 9 cut(s) 38, 387, 1227, 1440, 1571, 2088, 2681, 3177, 3770
HpyCH4IV ACGT 5 cut(s) 468, 1072, 1279, 2824, 3913
HpyF10VI GCNNNNNNNGC 4 cut(s) 1085, 1412, 2054, 3143
HpySE526I ACGT 5 cut(s) 468, 1072, 1279, 2824, 3913
Hsp92I GRCGYC 3 cut(s) 222, 399, 468
HspAI GCGC 6 cut(s) 125, 302, 520, 2965, 4054, 4164
Ksp22I TGATCA 1 cut(s) 1849
KspI CCGCGG 1 cut(s) 531
Kzo9I GATC 8 cut(s) 546, 1322, 1724, 1849, 2083, 2104, 3172, 3193
LmnI GCTCC 7 cut(s) 571, 1001, 1573, 1683, 1904, 2070, 3159
Lsp1109I GCAGC 5 cut(s) 1346, 2057, 3030, 3146, 4119
MaeII ACGT 5 cut(s) 468, 1072, 1279, 2824, 3913
MalI GATC 8 cut(s) 548, 1324, 1726, 1851, 2085, 2106, 3174, 3195
MboI GATC 8 cut(s) 546, 1322, 1724, 1849, 2083, 2104, 3172, 3193
MflI RGATCY 3 cut(s) 1322, 2083, 3172
MhlI GDGCHC 4 cut(s) 693, 998, 1063, 1377
MlyI GAGTC 4 cut(s) 565, 1115, 2531, 3620
MmeI TCCRAC 7 cut(s) 130, 507, 1933, 2048, 3022, 3137, 3934
MroXI GAANNNNTTC 3 cut(s) 1143, 2136, 3225
MslI CAYNNNNRTG 6 cut(s) 2259, 2424, 2949, 3348, 3513, 4038
MspA1I CMGCKG 1 cut(s) 530
MspI CCGG 6 cut(s) 2034, 2327, 2853, 3123, 3416, 3942
Mva1269I GAATGC 1 cut(s) 1206
MvaI CCWGG 8 cut(s) 82, 1065, 1383, 2529, 2550, 2969, 3618, 3639
MvnI CGCG 5 cut(s) 219, 396, 522, 530, 4166
MwoI GCNNNNNNNGC 4 cut(s) 1085, 1412, 2054, 3143
NciI CCSGG 7 cut(s) 2328, 2853, 2854, 3416, 3417, 3942, 3943
NcoI CCATGG 1 cut(s) 1783
NdeI CATATG 2 cut(s) 2142, 3231
NdeII GATC 8 cut(s) 546, 1322, 1724, 1849, 2083, 2104, 3172, 3193
NlaIV GGNNCC 8 cut(s) 1062, 1156, 2066, 2986, 3005, 3155, 4075, 4094
NmeAIII GCCGAG 4 cut(s) 250, 258, 427, 435
NspI RCATGY 2 cut(s) 1440, 1657
PaqCI CACCTGC 1 cut(s) 1216
PciI ACATGT 1 cut(s) 1436
PcsI WCGNNNNNNNCGW 1 cut(s) 462
PctI GAATGC 1 cut(s) 1206
PdmI GAANNNNTTC 3 cut(s) 1143, 2136, 3225
PfeI GAWTC 7 cut(s) 1082, 2030, 2461, 2896, 3119, 3550, 3985
PfoI TCCNGGA 2 cut(s) 2548, 3637
PkrI GCNGC 9 cut(s) 124, 301, 529, 532, 1336, 2047, 3020, 3136, 4109
PleI GAGTC 4 cut(s) 564, 1114, 2530, 3619
PpsI GAGTC 4 cut(s) 564, 1114, 2530, 3619
PscI ACATGT 1 cut(s) 1436
Psp6I CCWGG 8 cut(s) 80, 1063, 1381, 2527, 2548, 2967, 3616, 3637
PspFI CCCAGC 4 cut(s) 533, 949, 2989, 4078
PspGI CCWGG 8 cut(s) 80, 1063, 1381, 2527, 2548, 2967, 3616, 3637
PspN4I GGNNCC 8 cut(s) 1062, 1156, 2066, 2986, 3005, 3155, 4075, 4094
PspPI GGNCC 7 cut(s) 47, 1155, 1227, 1385, 1558, 2984, 4073
PstI CTGCAG 1 cut(s) 1686
PstNI CAGNNNCTG 1 cut(s) 1340
PsuI RGATCY 3 cut(s) 1322, 2083, 3172
RseI CAYNNNNRTG 6 cut(s) 2259, 2424, 2949, 3348, 3513, 4038
SacII CCGCGG 1 cut(s) 531
SatI GCNGC 9 cut(s) 123, 300, 528, 531, 1335, 2046, 3019, 3135, 4108
Sau3AI GATC 8 cut(s) 546, 1322, 1724, 1849, 2083, 2104, 3172, 3193
Sau96I GGNCC 7 cut(s) 47, 1155, 1227, 1385, 1558, 2984, 4073
ScaI AGTACT 3 cut(s) 679, 2449, 3538
SchI GAGTC 4 cut(s) 565, 1115, 2531, 3620
SduI GDGCHC 4 cut(s) 693, 998, 1063, 1377
SfcI CTRYAG 4 cut(s) 1682, 2112, 3201, 4175
Sfr303I CCGCGG 1 cut(s) 531
SgrBI CCGCGG 1 cut(s) 531
SinI GGWCC 4 cut(s) 47, 1155, 1385, 1558
SmaI CCCGGG 3 cut(s) 2854, 3417, 3943
SmiMI CAYNNNNRTG 6 cut(s) 2259, 2424, 2949, 3348, 3513, 4038
SspI AATATT 3 cut(s) 1834, 2005, 3094
StyI CCWWGG 6 cut(s) 567, 1783, 2280, 2768, 3369, 3857
TaiI ACGT 5 cut(s) 471, 1075, 1282, 2827, 3916
TaqI TCGA 5 cut(s) 8, 618, 1051, 2311, 3400
TatI WGTACW 6 cut(s) 677, 1246, 2447, 2890, 3536, 3979
TauI GCSGC 4 cut(s) 125, 302, 530, 533
TfiI GAWTC 7 cut(s) 1082, 2030, 2461, 2896, 3119, 3550, 3985
TscAI CASTG 6 cut(s) 1650, 1783, 1966, 2129, 3055, 3218
TseI GCWGC 5 cut(s) 1334, 2045, 3018, 3134, 4107
TspGWI ACGGA 8 cut(s) 124, 301, 1147, 2649, 2867, 3738, 3956, 4124
TspMI CCCGGG 3 cut(s) 2852, 3415, 3941
TspRI CASTG 6 cut(s) 1650, 1783, 1966, 2129, 3055, 3218
VneI GTGCAC 1 cut(s) 689
VpaK11BI GGWCC 4 cut(s) 47, 1155, 1385, 1558
XagI CCTNNNNNAGG 2 cut(s) 2532, 3621
XceI RCATGY 2 cut(s) 1440, 1657
XcmI CCANNNNNNNNNTGG 4 cut(s) 1718, 2766, 3855, 3917
XmaI CCCGGG 3 cut(s) 2852, 3415, 3941
XmnI GAANNNNTTC 3 cut(s) 1143, 2136, 3225
ZraI GACGTC 1 cut(s) 469
ZrmI AGTACT 3 cut(s) 679, 2449, 3538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.