Rmu_sc0002322.1_g000063

26S protease regulatory subunit 6B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002322.1
OG0016980
Physical Location & Seq
Reverse (-)
270870 .. 274532
3663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002322.1_g000063.1.cds

Sequence Viewer

Length: 435 bp
atggtccgtgatgtttttcgtctcgccaaggagaatgcacctgccatcatctttattgttaaggtggatgctattgctactgcaaggtttgatgctcaaactagagctgatagagaagttcagcgaattcttatggagctcctcaatcaggatgtcgagttgccaatcttcttgaatgataaaaggttgctatccacacaagtaaaagcacctgcccaagcaaggcaaatgaaatatgatgctgacccagcggcaaaggcagctgctgcaactgtcctgggcatccaagttgggtgtggattgcattcaggagatgaatctagagctaatgttgcccaagataatgaacttgtccatcagaatcctcaagcatatgctacacaaaatggaaattggattgctcgtattgcagtattacttgtgggtgaggattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

144

Amino Acids

15.79

Weight (kDa)

5.68

Isoelectric Point (pI)

22.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 49, 220
Acc36I ACCTGC 2 cut(s) 49, 220
AciI CCGC 1 cut(s) 251
AcsI RAATTY 1 cut(s) 126
AfiI CCNNNNNNNGG 2 cut(s) 148, 222
AgsI TTSAA 1 cut(s) 175
AjnI CCWGG 1 cut(s) 276
AluBI AGCT 4 cut(s) 107, 139, 263, 326
AluI AGCT 4 cut(s) 107, 139, 263, 326
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 1 cut(s) 26
AlwNI CAGNNNCTG 1 cut(s) 266
ApeKI GCWGC 3 cut(s) 260, 263, 266
ApoI RAATTY 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 4
AvaII GGWCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 3 cut(s) 250, 253, 272
BccI CCATC 2 cut(s) 53, 363
BciT130I CCWGG 1 cut(s) 278
BcoDI GTCTC 1 cut(s) 26
BfaI CTAG 2 cut(s) 102, 321
BfuAI ACCTGC 2 cut(s) 49, 220
BisI GCNGC 4 cut(s) 252, 261, 264, 267
BlsI GCNGC 4 cut(s) 253, 262, 265, 268
Bme1390I CCNGG 1 cut(s) 278
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmrFI CCNGG 1 cut(s) 278
BmsI GCATC 4 cut(s) 58, 82, 229, 291
BpuEI CTTGAG 1 cut(s) 351
BsaJI CCNNGG 2 cut(s) 27, 277
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 222
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 2 cut(s) 27, 277
BseGI GGATG 3 cut(s) 73, 157, 282
BseLI CCNNNNNNNGG 2 cut(s) 148, 222
BseRI GAGGAG 1 cut(s) 131
BseXI GCAGC 3 cut(s) 250, 253, 272
BseYI CCCAGC 1 cut(s) 247
BsiHKAI GWGCWC 1 cut(s) 141
BslI CCNNNNNNNGG 2 cut(s) 148, 222
BsmAI GTCTC 1 cut(s) 26
BsmBI CGTCTC 1 cut(s) 26
BsmI GAATGC 2 cut(s) 40, 304
Bsp1286I GDGCHC 1 cut(s) 141
BspACI CCGC 1 cut(s) 251
BspMI ACCTGC 2 cut(s) 49, 220
BssECI CCNNGG 2 cut(s) 27, 277
BssT1I CCWWGG 1 cut(s) 27
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 1 cut(s) 274
BstAPI GCANNNNNTGC 1 cut(s) 266
BstENI CCTNNNNNAGG 1 cut(s) 146
BstF5I GGATG 3 cut(s) 73, 157, 282
BstMAI GTCTC 1 cut(s) 26
BstMWI GCNNNNNNNGC 6 cut(s) 248, 257, 260, 266, 332, 407
BstNI CCWGG 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 276
BstV1I GCAGC 3 cut(s) 250, 253, 272
BtsCI GGATG 3 cut(s) 73, 157, 282
BveI ACCTGC 2 cut(s) 49, 220
CaiI CAGNNNCTG 1 cut(s) 266
Cfr13I GGNCC 1 cut(s) 4
CviJI RGCY 4 cut(s) 107, 139, 263, 326
CviKI_1 RGCY 4 cut(s) 107, 139, 263, 326
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 27
Eco24I GRGCYC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 4
Eco53kI GAGCTC 1 cut(s) 139
EcoICRI GAGCTC 1 cut(s) 139
EcoNI CCTNNNNNAGG 1 cut(s) 146
EcoRI GAATTC 1 cut(s) 126
EcoRII CCWGG 1 cut(s) 276
EcoT14I CCWWGG 1 cut(s) 27
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 27
Esp3I CGTCTC 1 cut(s) 26
FaiI YATR 4 cut(s) 134, 237, 373, 375
FauNDI CATATG 1 cut(s) 373
Fnu4HI GCNGC 4 cut(s) 252, 261, 264, 267
FokI GGATG 3 cut(s) 80, 164, 269
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 4 cut(s) 252, 261, 264, 267
FspBI CTAG 2 cut(s) 102, 321
GluI GCNGC 4 cut(s) 252, 261, 264, 267
GsaI CCCAGC 1 cut(s) 251
HinfI GANTC 2 cut(s) 317, 361
Hpy188I TCNGA 1 cut(s) 360
Hpy188III TCNNGA 4 cut(s) 149, 172, 309, 321
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4V TGCA 5 cut(s) 38, 83, 269, 304, 410
HpyF10VI GCNNNNNNNGC 6 cut(s) 248, 257, 260, 266, 332, 407
LmnI GCTCC 2 cut(s) 136, 144
LpnPI CCDG 7 cut(s) 54, 134, 225, 261, 263, 290, 294
Lsp1109I GCAGC 3 cut(s) 250, 253, 272
LweI GCATC 4 cut(s) 58, 82, 229, 291
MaeI CTAG 2 cut(s) 102, 321
MboII GAAGA 1 cut(s) 160
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 2 cut(s) 126, 391
MnlI CCTC 3 cut(s) 152, 375, 421
MseI TTAA 1 cut(s) 60
MspA1I CMGCKG 2 cut(s) 251, 263
MspR9I CCNGG 1 cut(s) 278
Mva1269I GAATGC 2 cut(s) 40, 304
MvaI CCWGG 1 cut(s) 278
MwoI GCNNNNNNNGC 6 cut(s) 248, 257, 260, 266, 332, 407
NdeI CATATG 1 cut(s) 373
PaqCI CACCTGC 2 cut(s) 49, 220
PctI GAATGC 2 cut(s) 40, 304
PfeI GAWTC 2 cut(s) 317, 361
PkrI GCNGC 4 cut(s) 253, 262, 265, 268
Psp124BI GAGCTC 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 276
PspFI CCCAGC 1 cut(s) 247
PspGI CCWGG 1 cut(s) 276
PspPI GGNCC 1 cut(s) 4
PstNI CAGNNNCTG 1 cut(s) 266
PvuII CAGCTG 1 cut(s) 263
SacI GAGCTC 1 cut(s) 141
SaqAI TTAA 1 cut(s) 60
SatI GCNGC 4 cut(s) 252, 261, 264, 267
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 1 cut(s) 278
SduI GDGCHC 1 cut(s) 141
SetI ASST 9 cut(s) 43, 66, 89, 109, 141, 188, 214, 265, 328
SfaNI GCATC 4 cut(s) 58, 82, 229, 291
SinI GGWCC 1 cut(s) 4
SmlI CTYRAG 1 cut(s) 366
SmoI CTYRAG 1 cut(s) 366
Sse9I AATT 2 cut(s) 126, 391
SsiI CCGC 1 cut(s) 251
SspMI CTAG 2 cut(s) 102, 321
SstI GAGCTC 1 cut(s) 141
StyD4I CCNGG 1 cut(s) 276
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 1 cut(s) 274
TaqI TCGA 1 cut(s) 156
TasI AATT 2 cut(s) 126, 391
TauI GCSGC 1 cut(s) 254
TfiI GAWTC 2 cut(s) 317, 361
Tru1I TTAA 1 cut(s) 60
Tru9I TTAA 1 cut(s) 60
TseI GCWGC 3 cut(s) 260, 263, 266
TspDTI ATGAA 3 cut(s) 245, 330, 360
VpaK11BI GGWCC 1 cut(s) 4
XagI CCTNNNNNAGG 1 cut(s) 146
XapI RAATTY 1 cut(s) 126
XbaI TCTAGA 1 cut(s) 320
XcmI CCANNNNNNNNNTGG 1 cut(s) 293
XspI CTAG 2 cut(s) 102, 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.