Rorug06G0295400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0016980
Physical Location & Seq
Reverse (-)
44758370 .. 44762524
4155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0295400.1

Sequence Viewer

Length: 3699 bp
ATGATCCAACATAACATCAACACGTCAACTTTGCAAGCCATGGGAGGTGTTCCAAGACCTCATCCTCTTCTCCTTCTGCTTCACATTTTCTTGCTCTCATGGCTTCCATTGAAGGCAACCTCATCACCCAGAACACAAGCAGAGGCTTTGGTTAGCTGGAAGGGCAGCTTTTTTGCTCCCCCACCTTCTCTCAGTTCATGGTCCCTCACAAACATCAACAGCCTCTGCAACTGGACCTCCATTGTCTGTGACCCCAAAACCAAAACAGTTTCCCAGATTGACCTCTCAAATTTCAACCTCACTGCAACTCTAACAGGACTGGACTTCACCCAGTTTCTGAATCTCACCCATTTCAACCTCAATGGCAACAATTTCACAGGGGCAATACCATCTGCCATTGGCAACCTCACCAAGCTCACAACTTTGGATTTGGGCAACAATCTTTTTGAACAGGAAGTTCCTGTGGAGATGGGGAAGCTAACCCAGGTTGAGTATTTTAGTCTCTACAGCAACAGTCTCACTGGTGCCATTCCATATCAGCTGGACAATCTCAAAAAGGTACAATATTTGCTTCTTGGAAGTAACTACTTAGAGCCTGCTGATTGGGCAAAATTCTCAGGCTTTCCTGTGTTGACATTCCTTGATCTTTCTCTGAATAGTTTAGATTCAAAATTCCCAGAATTTATATCTGAATGTAGGAATTTGACATTCCTGGATTTGTCTCAAAATACTTTGACTGGCCAAATACCAGAAGTGGTATTTACCAATCTGGTCAAGCTTGAATATCTCAATCTCACCAATAACCAATTCCAAGGGCCAATGCCATATAACTTTCCCAACCTTAAACACCTTTATTTGGGATTGAACGACTTTAGTGGTCCAATTCCTGAAGACATAGGTCTGTTATCTGGTCTGGAAATTTTTGAAGTGCTTAACAATTCCCTTGAAGGGAAAATCCCATCCTCTATAGGCCAACTCAAGGAGCTTCAGCACCTTGATTTTTCATCAAACTCATTAAACTCTTCAATGCCTTCTGAGCTTGGTTCTTGTACCAACCTCACTTTCTTGGCCTTGGCTGTGAACAAACTCAGTGGGGAATTGCCTCTGTCCTTGTCAAAATTGACCAACTTGGTGGATTTGGGATTATCTGATAATCAATTTACTGGGCCAATCTTGCCTTCTCTGGTTTCCAATTGGACTGCAATAGCCTCTATTCAATTCCAAAACAATAGTTTCAGTGGCAACATTCCAGCAGCAATTGGCCTATTGACAAATCTTAATATCCTCTTTCTGTACAAAAATAATTTCACCGGCCCAATTCCCTCAGAGATAGGAAACTTGCAAGCTATGACAAGTTTGGATCTGTCGGGAAACCAGCTCTCCGGGCCTATTCCTATGGCATTCTGGAATCTCACTAATCTTCAAAGTGTACAGCTTTTCTCCAATAACCTCAGTGGCATAATCCGCCCAGAGATTGGGAACATGATTTCATTGTCTGTCTTTGATGTCAACACAAACCAATTTGAGGGGGAGTTGCCAGAGACCATTTCTCTCCTCACTAAGCTCGAGGGCTTTTCTGTGTTCACCAATAAGTTGTCCGGAACTATTCCTAGTGATTTTGGGAAGTATAGTCCTAAATTGGGATATCTTAGCTTTTCAAACAACAGCTTCTCTGGAGAATTGCCACCAGAGTTGTGCAGTGGATTTTCTTTGCAAGTATTCACAGTGAATGTCAACAACTTCACAGGGCCATTACCCGAGTGCTTGAGAAATTGTACTGCATTAAATAGAATCCGGCTTGATGAGAACCAATTCACCGGAAATATTACCAATGCATTTGGTGTCCATCCCATTCTTGAAGAAATTTATCTTAGTCACAACAAGTTTGTTGGTGAACTCTCACCACATTGGGGAGAGTGCATAAACATCACTGACATGCGTATGGACCGAAACAGAATTTCTGGTCAGATTCCACCTGAGCTTCTGAAGTTGAAGAACTTGCAGTATCTAACACTTGGTTCTAATGAATTCAGTGGGGAATTTCCAGTTGGTATTGGGAATCTAAGCTTGTTATACACTCTCAATCTCAGCAGGAACCACTTCACAGGAACCATCCCGCAGATCCACCAATTGAATAAGCTCCAGACTCTTGATTTGTCTGACAACAATTTTACAGGGGTAATACCAGTGGAACCTGGCACATTTGAGAGCTTAACAAGCTTAAACTTGAGCCACAACAAGTTATCAGGAGAAATACCAGCAGAGATTGGTAACTCAGAATTGAGGTACTTGTTGGATCTTAGTAGCAATTTGCTCTCAGGAGACATACCTTCAAACTTGGGCAAGCTCACACAGTTGGTGGTTCTCAATGTCTCACACAACAATCTGTCAGGGGACATACCATCAGCATTTTCCAACATGCTTAGTCTAGACAGCTATGATTTTTCTTACAACAACTTGACTGGTCCAATCCCAACTGGTGGAATTTTCCAAACTGCACCAGCAAATGCTTTTTTTGGCAACTCTGGTTTGTGTGGAGGTGCAGGACTAACAGCATGCAATTCCTCTAGTGGAAAGTCCAACAAGAACAACAAGAAAGTTCTTATTGGAGTCCTTGTTCCTATTTGTAGTTTAATAGTGATTGTAACTGTTATTGCTCTGATCCTTATTTTCCGTAAGAAACCTAAGCCGCTTGATGAGGAAACCAAAAGTTCTAAGAAGTCTGAGAGTTTTGAGTCGAATATATGGGAAAGAGAAGTAAAGTTTACATTTGGGGAAATTGTGAAGGCCACAGAGGACTTTGATGAGAATTACTGCATTGGAAAAGGAGGGTTTGGAAGAGTGTACAAAGCTGAGCTGCTTTCAGGTCAAATTGTTGCAGTCAAGAAGCTAAACATGTCTGACTCTAGTGACATTCCAGCAATAAATCGACAAAGTTTTGAGAATGAAATTCGAACTTTGACACATGTCAGGCACCGGAACATCATCAAGCTATTTGGGTTCTGTTCAAGGAGAGGTTCCATGTTCTTGGTTTATGAGTATTTAGAGAGAGGCAGTCTCGGAAAAGCATTATATGGGGTTGAAGGGAATGCAGAACTTGACTGGGCTACAAGGGTCAGAATTGTGCAAGGACTAGCTCATGCTCTTTCTTACCTGCACAATGACTGCTCTCCACCAGTTGTACACCGGGATGTTTCTATCAACAATGTACTGCTTGAGTGGGATTTTGAGCCCCGGCTATCAGATTTCGGAACAGCAAGACTGTTGAGTCTGGATTCATCCAACTGGACCAGTGCTGCTGGTTCTTATGGCTACATGGCACCTGAGCTCGCATACACTATGAAGGTGACAGATAAGTGTGATGTATATAGCTTTGGAGTGCTAGCACTAGAAGTCCTGATGGGAAGGCACCCAGGGGAAGTGCTAGAAGCTCTACTAGAGTCATCAAAATCATTGCAGGACAACACAGAAATGCTTTTGAAAGATGTGTTAGACCAAAGGCTGGAGCCTCCAACCGGTGAATTGGCTGAGGCAGTGGTGTTTGCAGTGAGCGTAGGCTTGGCCTGCACACGTTCCCGTCCAGAGTTACGACCCACAATGCGTTTTGTGGCACAAGAAATATCGGCTCGGACACAGCCTTACATTTCTGAACCATTTGGTACGTTAACCATCAACAAACTAACTGGTCTTCAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1232

Amino Acids

135.41

Weight (kDa)

5.28

Isoelectric Point (pI)

33.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 45 - 84 2e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 186 - 245 2.6e-07 Leucine rich repeat
LRR_8 PF13855 211 - 270 5.9e-08 Leucine rich repeat
LRR_14 PF23598 280 - 383 3.4e-09 Leucine-rich repeat region
LRR_14 PF23598 419 - 522 6.2e-06 Leucine-rich repeat region
LRR_14 PF23598 660 - 743 4.4e-06 Leucine-rich repeat region
LRR_8 PF13855 691 - 748 2.8e-07 Leucine rich repeat
Pkinase PF00069 938 - 1154 1.5e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 939 - 1206 5.3e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3267
Acc36I ACCTGC 1 cut(s) 3162
AccB1I GGYRCC 4 cut(s) 524, 2973, 3319, 3408
AccB7I CCANNNNNTGG 3 cut(s) 1475, 2480, 3502
AccIII TCCGGA 1 cut(s) 1598
AciI CCGC 3 cut(s) 1465, 2117, 2690
AclWI GGATC 4 cut(s) 1368, 2116, 2304, 2656
AcoI YGGCCR 1 cut(s) 739
AcuI CTGAAG 3 cut(s) 909, 971, 2006
AflIII ACRYGT 4 cut(s) 21, 2895, 2965, 3569
AgeI ACCGGT 1 cut(s) 3515
AjiI CACGTC 1 cut(s) 24
AjnI CCWGG 4 cut(s) 483, 711, 2194, 3412
AjuI GAANNNNNNNTTGG 8 cut(s) 791, 823, 1046, 1078, 1162, 1194, 2031, 2063
AloI GAACNNNNNNTCC 2 cut(s) 2601, 2633
Alw21I GWGCWC 1 cut(s) 3330
Alw26I GTCTC 7 cut(s) 506, 521, 726, 1535, 2316, 2377, 3062
AlwI GGATC 4 cut(s) 1368, 2116, 2304, 2656
AlwNI CAGNNNCTG 2 cut(s) 225, 337
Ama87I CYCGRG 2 cut(s) 1565, 1757
Aor13HI TCCGGA 1 cut(s) 1598
ApeKI GCWGC 4 cut(s) 165, 1253, 2857, 3296
ArsI GACNNNNNNTTYG 2 cut(s) 2137, 2169
AsiGI ACCGGT 1 cut(s) 3515
Asp700I GAANNNNTTC 2 cut(s) 1811, 2099
AsuC2I CCSGG 3 cut(s) 1384, 3188, 3235
AsuII TTCGAA 1 cut(s) 2953
AsuNHI GCTAGC 1 cut(s) 3382
AvaI CYCGRG 2 cut(s) 1565, 1757
AvaII GGWCC 6 cut(s) 201, 234, 878, 1945, 2465, 3288
BalI TGGCCA 1 cut(s) 741
BanI GGYRCC 4 cut(s) 524, 2973, 3319, 3408
BanII GRGCYC 2 cut(s) 3234, 3330
BbsI GAAGAC 2 cut(s) 897, 3680
Bbv12I GWGCWC 1 cut(s) 3330
BbvCI CCTCAGC 1 cut(s) 3528
BbvI GCAGC 4 cut(s) 177, 1265, 2844, 3283
BccI CCATC 8 cut(s) 397, 463, 967, 1854, 2120, 2410, 3394, 3677
BciT130I CCWGG 4 cut(s) 485, 713, 2196, 3414
BcnI CCSGG 3 cut(s) 1384, 3188, 3235
BcoDI GTCTC 7 cut(s) 506, 521, 726, 1535, 2316, 2377, 3062
BfaI CTAG 9 cut(s) 1611, 2429, 2568, 2907, 3134, 3383, 3389, 3425, 3437
BfmI CTRYAG 2 cut(s) 505, 966
BfuAI ACCTGC 1 cut(s) 3162
BisI GCNGC 5 cut(s) 166, 1254, 2690, 2858, 3297
BlpI GCTNAGC 1 cut(s) 2853
BlsI GCNGC 5 cut(s) 167, 1255, 2691, 2859, 3298
Bme1390I CCNGG 7 cut(s) 485, 713, 1384, 2196, 3188, 3235, 3414
Bme18I GGWCC 6 cut(s) 201, 234, 878, 1945, 2465, 3288
BmeT110I CYCGRG 2 cut(s) 1565, 1757
BmgBI CACGTC 1 cut(s) 24
BmrFI CCNGG 7 cut(s) 485, 713, 1384, 2196, 3188, 3235, 3414
BmrI ACTGGG 3 cut(s) 325, 1173, 3112
BmtI GCTAGC 1 cut(s) 3386
BmuI ACTGGG 3 cut(s) 325, 1173, 3112
BoxI GACNNNNGTC 2 cut(s) 897, 2966
BpiI GAAGAC 2 cut(s) 897, 3680
BplI GAGNNNNNCTC 2 cut(s) 3042, 3074
BpmI CTGGAG 3 cut(s) 1695, 2126, 3524
Bpu10I CCTNAGC 4 cut(s) 1977, 2685, 3324, 3528
Bpu1102I GCTNAGC 1 cut(s) 2853
Bpu14I TTCGAA 1 cut(s) 2953
BpuEI CTTGAG 4 cut(s) 962, 1786, 2248, 3236
BpuMI CCSGG 3 cut(s) 1384, 3188, 3235
BsaI GGTCTC 1 cut(s) 1535
BsaJI CCNNGG 7 cut(s) 39, 483, 811, 1071, 3233, 3412, 3413
BsaWI WCCGGW 4 cut(s) 1598, 1817, 2976, 3515
BsaXI ACNNNNNCTCC 6 cut(s) 221, 251, 1364, 1394, 2601, 2631
Bse118I RCCGGY 2 cut(s) 1310, 3515
Bse3DI GCAATG 1 cut(s) 3452
BseAI TCCGGA 1 cut(s) 1598
BseBI CCWGG 4 cut(s) 485, 713, 2196, 3414
BseDI CCNNGG 7 cut(s) 39, 483, 811, 1071, 3233, 3412, 3413
BseGI GGATG 6 cut(s) 61, 959, 1846, 2112, 3196, 3278
BseMI GCAATG 1 cut(s) 3452
BseRI GAGGAG 1 cut(s) 1544
BseXI GCAGC 4 cut(s) 177, 1265, 2844, 3283
BsgI GTGCAG 5 cut(s) 1717, 2481, 2562, 3140, 3550
BshNI GGYRCC 4 cut(s) 524, 2973, 3319, 3408
BshTI ACCGGT 1 cut(s) 3515
BsiHKAI GWGCWC 1 cut(s) 3330
BsiHKCI CYCGRG 2 cut(s) 1565, 1757
BsiSI CCGG 9 cut(s) 1311, 1383, 1599, 1795, 1818, 2977, 3187, 3235, 3516
BslFI GGGAC 2 cut(s) 187, 2408
BsmAI GTCTC 7 cut(s) 506, 521, 726, 1535, 2316, 2377, 3062
BsmFI GGGAC 2 cut(s) 187, 2408
BsmI GAATGC 2 cut(s) 1400, 3094
Bso31I GGTCTC 1 cut(s) 1535
BsoBI CYCGRG 2 cut(s) 1565, 1757
Bsp119I TTCGAA 1 cut(s) 2953
Bsp1286I GDGCHC 2 cut(s) 3234, 3330
Bsp13I TCCGGA 1 cut(s) 1598
Bsp1407I TGTACA 4 cut(s) 1293, 1429, 2844, 3181
Bsp143I GATC 6 cut(s) 3, 643, 1360, 2121, 2296, 2661
Bsp1720I GCTNAGC 1 cut(s) 2853
Bsp19I CCATGG 1 cut(s) 39
BspACI CCGC 3 cut(s) 1465, 2117, 2690
BspEI TCCGGA 1 cut(s) 1598
BspMI ACCTGC 1 cut(s) 3162
BspOI GCTAGC 1 cut(s) 3386
BspPI GGATC 4 cut(s) 1368, 2116, 2304, 2656
BspT104I TTCGAA 1 cut(s) 2953
BspT107I GGYRCC 4 cut(s) 524, 2973, 3319, 3408
BspTNI GGTCTC 1 cut(s) 1535
BsrDI GCAATG 1 cut(s) 3452
BsrFI RCCGGY 2 cut(s) 1310, 3515
BsrGI TGTACA 4 cut(s) 1293, 1429, 2844, 3181
BssAI RCCGGY 2 cut(s) 1310, 3515
BssECI CCNNGG 7 cut(s) 39, 483, 811, 1071, 3233, 3412, 3413
BssMI GATC 6 cut(s) 3, 643, 1360, 2121, 2296, 2661
BssT1I CCWWGG 3 cut(s) 39, 811, 1071
Bst2UI CCWGG 4 cut(s) 485, 713, 2196, 3414
Bst4CI ACNGT 6 cut(s) 268, 515, 1726, 2355, 2650, 3264
Bst6I CTCTTC 3 cut(s) 72, 1027, 2833
BstAUI TGTACA 4 cut(s) 1293, 1429, 2844, 3181
BstBI TTCGAA 1 cut(s) 2953
BstC8I GCNNGC 8 cut(s) 36, 597, 1344, 2345, 2557, 3330, 3384, 3565
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 6 cut(s) 61, 959, 1846, 2112, 3196, 3278
BstKTI GATC 6 cut(s) 6, 646, 1363, 2124, 2299, 2664
BstMAI GTCTC 7 cut(s) 506, 521, 726, 1535, 2316, 2377, 3062
BstMBI GATC 6 cut(s) 3, 643, 1360, 2121, 2296, 2661
BstMWI GCNNNNNNNGC 9 cut(s) 100, 162, 605, 1036, 1174, 1384, 1464, 2217, 3564
BstNI CCWGG 4 cut(s) 485, 713, 2196, 3414
BstNSI RCATGY 5 cut(s) 1939, 2422, 2559, 2899, 2969
BstPAI GACNNNNGTC 2 cut(s) 897, 2966
BstSCI CCNGG 7 cut(s) 483, 711, 1382, 2194, 3186, 3233, 3412
BstSFI CTRYAG 2 cut(s) 505, 966
BstV1I GCAGC 4 cut(s) 177, 1265, 2844, 3283
BstV2I GAAGAC 2 cut(s) 897, 3680
BstX2I RGATCY 3 cut(s) 1360, 2121, 2296
BstXI CCANNNNNNTGG 2 cut(s) 1132, 3028
BstYI RGATCY 3 cut(s) 1360, 2121, 2296
BtgI CCRYGG 1 cut(s) 39
BtrI CACGTC 1 cut(s) 24
BtsCI GGATG 6 cut(s) 61, 959, 1846, 2112, 3196, 3278
BtsI GCAGTG 4 cut(s) 300, 1705, 3540, 3552
BveI ACCTGC 1 cut(s) 3162
Cac8I GCNNGC 8 cut(s) 36, 597, 1344, 2345, 2557, 3330, 3384, 3565
CaiI CAGNNNCTG 2 cut(s) 225, 337
Cfr10I RCCGGY 2 cut(s) 1310, 3515
CspAI ACCGGT 1 cut(s) 3515
CspCI CAANNNNNGTGG 4 cut(s) 1072, 1107, 1674, 1709
DpnI GATC 6 cut(s) 5, 645, 1362, 2123, 2298, 2663
DpnII GATC 6 cut(s) 3, 643, 1360, 2121, 2296, 2661
DrdI GACNNNNNNGTC 1 cut(s) 3267
DseDI GACNNNNNNGTC 1 cut(s) 3267
EaeI YGGCCR 1 cut(s) 739
Eam1104I CTCTTC 3 cut(s) 72, 1027, 2833
EarI CTCTTC 3 cut(s) 72, 1027, 2833
EciI GGCGGA 1 cut(s) 1454
Ecl136II GAGCTC 1 cut(s) 3328
Eco130I CCWWGG 3 cut(s) 39, 811, 1071
Eco24I GRGCYC 2 cut(s) 3234, 3330
Eco31I GGTCTC 1 cut(s) 1535
Eco32I GATATC 1 cut(s) 1646
Eco47I GGWCC 6 cut(s) 201, 234, 878, 1945, 2465, 3288
Eco53kI GAGCTC 1 cut(s) 3328
Eco57I CTGAAG 3 cut(s) 909, 971, 2006
Eco88I CYCGRG 2 cut(s) 1565, 1757
EcoICRI GAGCTC 1 cut(s) 3328
EcoRI GAATTC 1 cut(s) 2027
EcoRII CCWGG 4 cut(s) 483, 711, 2194, 3412
EcoRV GATATC 1 cut(s) 1646
EcoT14I CCWWGG 3 cut(s) 39, 811, 1071
EcoT22I ATGCAT 1 cut(s) 1837
EcoT38I GRGCYC 2 cut(s) 3234, 3330
ErhI CCWWGG 3 cut(s) 39, 811, 1071
FalI AAGNNNNNCTT 2 cut(s) 152, 184
FaqI GGGAC 2 cut(s) 187, 2408
FauI CCCGC 1 cut(s) 2124
Fnu4HI GCNGC 5 cut(s) 166, 1254, 2690, 2858, 3297
FokI GGATG 6 cut(s) 48, 946, 1833, 2099, 3203, 3265
FriOI GRGCYC 2 cut(s) 3234, 3330
Fsp4HI GCNGC 5 cut(s) 166, 1254, 2690, 2858, 3297
FspBI CTAG 9 cut(s) 1611, 2429, 2568, 2907, 3134, 3383, 3389, 3425, 3437
GluI GCNGC 5 cut(s) 166, 1254, 2690, 2858, 3297
GsuI CTGGAG 3 cut(s) 1695, 2126, 3524
HapII CCGG 9 cut(s) 1311, 1383, 1599, 1795, 1818, 2977, 3187, 3235, 3516
HincII GTYRAC 5 cut(s) 27, 633, 1510, 1735, 3666
HindII GTYRAC 5 cut(s) 27, 633, 1510, 1735, 3666
HindIII AAGCTT 3 cut(s) 776, 2065, 2218
HpaI GTTAAC 1 cut(s) 3666
HpaII CCGG 9 cut(s) 1311, 1383, 1599, 1795, 1818, 2977, 3187, 3235, 3516
HpyCH4III ACNGT 6 cut(s) 268, 515, 1726, 2355, 2650, 3264
HpyCH4IV ACGT 3 cut(s) 23, 3571, 3662
HpyF10VI GCNNNNNNNGC 9 cut(s) 100, 162, 605, 1036, 1174, 1384, 1464, 2217, 3564
HpySE526I ACGT 3 cut(s) 23, 3571, 3662
Kpn2I TCCGGA 1 cut(s) 1598
KspAI GTTAAC 1 cut(s) 3666
Kzo9I GATC 6 cut(s) 3, 643, 1360, 2121, 2296, 2661
LmnI GCTCC 4 cut(s) 181, 982, 2145, 3505
Lsp1109I GCAGC 4 cut(s) 177, 1265, 2844, 3283
MaeI CTAG 9 cut(s) 1611, 2429, 2568, 2907, 3134, 3383, 3389, 3425, 3437
MaeII ACGT 3 cut(s) 23, 3571, 3662
MaeIII GTNAC 8 cut(s) 248, 581, 1874, 2270, 2644, 2909, 3346, 3585
MalI GATC 6 cut(s) 5, 645, 1362, 2123, 2298, 2663
MboI GATC 6 cut(s) 3, 643, 1360, 2121, 2296, 2661
MboII GAAGA 8 cut(s) 59, 902, 1014, 1412, 1871, 2005, 2850, 3680
MfeI CAATTG 3 cut(s) 1192, 1257, 2129
MflI RGATCY 3 cut(s) 1360, 2121, 2296
MhlI GDGCHC 2 cut(s) 3234, 3330
MlsI TGGCCA 1 cut(s) 741
MluNI TGGCCA 1 cut(s) 741
MlyI GAGTC 6 cut(s) 2140, 2619, 2744, 2897, 3277, 3449
MmeI TCCRAC 6 cut(s) 31, 2274, 2439, 2604, 3306, 3536
Mox20I TGGCCA 1 cut(s) 741
Mph1103I ATGCAT 1 cut(s) 1837
MroI TCCGGA 1 cut(s) 1598
MroXI GAANNNNTTC 2 cut(s) 1811, 2099
MscI TGGCCA 1 cut(s) 741
MseI TTAA 9 cut(s) 843, 933, 1016, 1278, 1784, 2213, 2222, 2633, 3665
MslI CAYNNNNRTG 4 cut(s) 1934, 1940, 3189, 3470
Msp20I TGGCCA 1 cut(s) 741
MspA1I CMGCKG 1 cut(s) 541
MspI CCGG 9 cut(s) 1311, 1383, 1599, 1795, 1818, 2977, 3187, 3235, 3516
MspR9I CCNGG 7 cut(s) 485, 713, 1384, 2196, 3188, 3235, 3414
MunI CAATTG 3 cut(s) 1192, 1257, 2129
Mva1269I GAATGC 2 cut(s) 1400, 3094
MvaI CCWGG 4 cut(s) 485, 713, 2196, 3414
MwoI GCNNNNNNNGC 9 cut(s) 100, 162, 605, 1036, 1174, 1384, 1464, 2217, 3564
NciI CCSGG 3 cut(s) 1384, 3188, 3235
NcoI CCATGG 1 cut(s) 39
NdeII GATC 6 cut(s) 3, 643, 1360, 2121, 2296, 2661
NheI GCTAGC 1 cut(s) 3382
NmuCI GTSAC 4 cut(s) 248, 1874, 2909, 3346
NsiI ATGCAT 1 cut(s) 1837
NspI RCATGY 5 cut(s) 1939, 2422, 2559, 2899, 2969
NspV TTCGAA 1 cut(s) 2953
PaeI GCATGC 1 cut(s) 2559
PaeR7I CTCGAG 1 cut(s) 1565
PasI CCCWGGG 1 cut(s) 3413
PciI ACATGT 2 cut(s) 2895, 2965
PctI GAATGC 2 cut(s) 1400, 3094
PdmI GAANNNNTTC 2 cut(s) 1811, 2099
PfeI GAWTC 7 cut(s) 340, 665, 1408, 1791, 1969, 2059, 3275
PflMI CCANNNNNTGG 3 cut(s) 1475, 2480, 3502
PfoI TCCNGGA 1 cut(s) 711
PinAI ACCGGT 1 cut(s) 3515
PkrI GCNGC 5 cut(s) 167, 1255, 2691, 2859, 3298
PleI GAGTC 6 cut(s) 2140, 2618, 2743, 2897, 3276, 3448
PpsI GAGTC 6 cut(s) 2140, 2618, 2743, 2897, 3276, 3448
PscI ACATGT 2 cut(s) 2895, 2965
PshAI GACNNNNGTC 2 cut(s) 897, 2966
Psp124BI GAGCTC 1 cut(s) 3330
Psp6I CCWGG 4 cut(s) 483, 711, 2194, 3412
PspGI CCWGG 4 cut(s) 483, 711, 2194, 3412
PspXI VCTCGAGB 1 cut(s) 1565
PstNI CAGNNNCTG 2 cut(s) 225, 337
PsuI RGATCY 3 cut(s) 1360, 2121, 2296
PvuII CAGCTG 1 cut(s) 541
RseI CAYNNNNRTG 4 cut(s) 1934, 1940, 3189, 3470
SacI GAGCTC 1 cut(s) 3330
SaqAI TTAA 9 cut(s) 843, 933, 1016, 1278, 1784, 2213, 2222, 2633, 3665
SatI GCNGC 5 cut(s) 166, 1254, 2690, 2858, 3297
Sau3AI GATC 6 cut(s) 3, 643, 1360, 2121, 2296, 2661
SchI GAGTC 6 cut(s) 2140, 2619, 2744, 2897, 3277, 3449
ScrFI CCNGG 7 cut(s) 485, 713, 1384, 2196, 3188, 3235, 3414
SduI GDGCHC 2 cut(s) 3234, 3330
SfcI CTRYAG 2 cut(s) 505, 966
Sfr274I CTCGAG 1 cut(s) 1565
SfuI TTCGAA 1 cut(s) 2953
SinI GGWCC 6 cut(s) 201, 234, 878, 1945, 2465, 3288
SlaI CTCGAG 1 cut(s) 1565
SmiMI CAYNNNNRTG 4 cut(s) 1934, 1940, 3189, 3470
SmlI CTYRAG 5 cut(s) 977, 1565, 1765, 2227, 3215
SmoI CTYRAG 5 cut(s) 977, 1565, 1765, 2227, 3215
SphI GCATGC 1 cut(s) 2559
SsiI CCGC 3 cut(s) 1465, 2117, 2690
SspI AATATT 2 cut(s) 566, 1825
SspMI CTAG 9 cut(s) 1611, 2429, 2568, 2907, 3134, 3383, 3389, 3425, 3437
SstI GAGCTC 1 cut(s) 3330
StyD4I CCNGG 7 cut(s) 483, 711, 1382, 2194, 3186, 3233, 3412
StyI CCWWGG 3 cut(s) 39, 811, 1071
TaaI ACNGT 6 cut(s) 268, 515, 1726, 2355, 2650, 3264
TaiI ACGT 3 cut(s) 26, 3574, 3665
TaqI TCGA 4 cut(s) 1566, 2738, 2929, 2953
TaqII GACCGA 1 cut(s) 1962
TatI WGTACW 6 cut(s) 1293, 1429, 1775, 2844, 3181, 3208
TauI GCSGC 1 cut(s) 2692
TfiI GAWTC 7 cut(s) 340, 665, 1408, 1791, 1969, 2059, 3275
Tru1I TTAA 9 cut(s) 843, 933, 1016, 1278, 1784, 2213, 2222, 2633, 3665
Tru9I TTAA 9 cut(s) 843, 933, 1016, 1278, 1784, 2213, 2222, 2633, 3665
TseFI GTSAC 4 cut(s) 248, 1874, 2909, 3346
TseI GCWGC 4 cut(s) 165, 1253, 2857, 3296
Tsp45I GTSAC 4 cut(s) 248, 1874, 2909, 3346
TspDTI ATGAA 7 cut(s) 186, 993, 1479, 2040, 2961, 3267, 3356
TspGWI ACGGA 1 cut(s) 2663
Van91I CCANNNNNTGG 3 cut(s) 1475, 2480, 3502
VpaK11BI GGWCC 6 cut(s) 201, 234, 878, 1945, 2465, 3288
XbaI TCTAGA 1 cut(s) 2428
XceI RCATGY 5 cut(s) 1939, 2422, 2559, 2899, 2969
XhoI CTCGAG 1 cut(s) 1565
XmnI GAANNNNTTC 2 cut(s) 1811, 2099
XspI CTAG 9 cut(s) 1611, 2429, 2568, 2907, 3134, 3383, 3389, 3425, 3437
Zsp2I ATGCAT 1 cut(s) 1837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.