Rmu_sc0002909.1_g000020

Protein unc-45 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002909.1
OG0010186
Physical Location & Seq
Forward (+)
117960 .. 120589
2630 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 969 bp
ctgtctcagctccgcctctccagctttgtaaatcacatttcacagtcactactccatccccaaactccgtctctctccgatagactgaacagctggacggctgagattggccggatggcatcggcggcgccggcgaacaagatcgagcgggcccacctcatgtaccgggaagggaggtaccaggaggccctgggattctacaccgaagccctgtccatggccaagaccaaaccccagaagattgccctccacagcaacagagccgcttgcttcctcaagcttcacgatttcaaaaaggctgcagaggaatgtacctcagtgcttgagcttgattacaatcacactggagcattgatgttgcgggctcagaccctggtgacactaaaggagtaccgttcagcactctttgatgttaataggctcatagagttaaatccatcatccgaagtgtatcagaaccttgaagcccgtttaaggacgcaagtgtcactggctccaatacccgagtccgaagcagagctagaagaagaggaagaagaagaggatgaagcagggccaaatagatatggggagagagaagagcaatatataggaaaagaagatgtagtacctttgagggaaggaagggatcagcttgctgaggttaataggactactattgctgatgtcatcccactcaagacaccaacaaaccaggactctgctgaacaagtaagggatccaaaagaaaactctattaagactacttccccggctgaagttacaccagataacatcaaagtatcacctgaaaaagaaaagggatggcaagcaatcccaaaaccaaagggacactcttctctggattatggaaggtgggacagtgtggaggatgactctagcgaagatgatgacgatgatgatgaggaagagtctcgaccacagtatcggttccgtgtaaaaactgttggtgttcggccggtaaagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_16 TPR_19 TPR_8

Protein Analysis

322

Amino Acids

36.52

Weight (kDa)

4.96

Isoelectric Point (pI)

53.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC3 PF12895 58 - 135 3.5e-06 Anaphase-promoting complex, cyclosome, subunit 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 484
Acc65I GGTACC 1 cut(s) 177
AccB1I GGYRCC 2 cut(s) 127, 177
AccBSI CCGCTC 1 cut(s) 148
AciI CCGC 5 cut(s) 13, 125, 148, 264, 361
AclWI GGATC 3 cut(s) 636, 713, 726
AcoI YGGCCR 3 cut(s) 109, 219, 956
AcuI CTGAAG 1 cut(s) 777
AcyI GRCGYC 1 cut(s) 128
AfaI GTAC 5 cut(s) 164, 179, 313, 392, 609
AfiI CCNNNNNNNGG 2 cut(s) 217, 474
AgsI TTSAA 2 cut(s) 292, 464
AjnI CCWGG 4 cut(s) 180, 189, 372, 693
AluBI AGCT 7 cut(s) 10, 24, 93, 280, 328, 520, 634
AluI AGCT 7 cut(s) 10, 24, 93, 280, 328, 520, 634
Alw26I GTCTC 3 cut(s) 9, 75, 918
AlwI GGATC 3 cut(s) 636, 713, 726
AlwNI CAGNNNCTG 1 cut(s) 373
Ama87I CYCGRG 1 cut(s) 503
AoxI GGCC 6 cut(s) 109, 150, 186, 219, 554, 956
ApaI GGGCCC 1 cut(s) 154
ApeKI GCWGC 1 cut(s) 299
Asp718I GGTACC 1 cut(s) 177
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 4 cut(s) 150, 151, 187, 554
AsuC2I CCSGG 2 cut(s) 167, 752
AsuHPI GGTGA 2 cut(s) 388, 777
AvaI CYCGRG 1 cut(s) 503
BaeGI GKGCMC 1 cut(s) 154
BaeI ACNNNNGTAYC 2 cut(s) 908, 941
BalI TGGCCA 1 cut(s) 221
BamHI GGATCC 1 cut(s) 718
BanI GGYRCC 2 cut(s) 127, 177
BanII GRGCYC 2 cut(s) 154, 367
BarI GAAGNNNNNNTAC 2 cut(s) 591, 623
BbvCI CCTCAGC 1 cut(s) 639
BbvI GCAGC 1 cut(s) 286
BccI CCATC 4 cut(s) 63, 109, 445, 798
BceAI ACGGC 1 cut(s) 114
BciT130I CCWGG 4 cut(s) 182, 191, 374, 695
BcnI CCSGG 2 cut(s) 167, 752
BcoDI GTCTC 3 cut(s) 9, 75, 918
BfaI CTAG 2 cut(s) 521, 879
BfmI CTRYAG 1 cut(s) 300
BfoI RGCGCY 1 cut(s) 131
BisI GCNGC 3 cut(s) 126, 264, 300
BlsI GCNGC 3 cut(s) 127, 265, 301
Bme1390I CCNGG 6 cut(s) 167, 182, 191, 374, 695, 752
BmeT110I CYCGRG 1 cut(s) 503
BmgT120I GGNCC 4 cut(s) 150, 151, 187, 554
BmiI GGNNCC 6 cut(s) 129, 152, 179, 495, 720, 932
BmrFI CCNGG 6 cut(s) 167, 182, 191, 374, 695, 752
BmsI GCATC 1 cut(s) 128
BplI GAGNNNNNCTC 2 cut(s) 860, 892
BpmI CTGGAG 2 cut(s) 4, 366
Bpu10I CCTNAGC 1 cut(s) 639
BpuEI CTTGAG 3 cut(s) 260, 344, 662
BpuMI CCSGG 2 cut(s) 167, 752
BsaBI GATNNNNATC 1 cut(s) 336
BsaHI GRCGYC 1 cut(s) 128
BsaJI CCNNGG 5 cut(s) 189, 190, 216, 372, 750
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 474
Bse118I RCCGGY 2 cut(s) 130, 958
Bse1I ACTGG 2 cut(s) 349, 495
Bse8I GATNNNNATC 1 cut(s) 336
BseBI CCWGG 4 cut(s) 182, 191, 374, 695
BseDI CCNNGG 5 cut(s) 189, 190, 216, 372, 750
BseGI GGATG 7 cut(s) 55, 120, 440, 550, 669, 809, 877
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 2 cut(s) 217, 474
BseMII CTCAG 5 cut(s) 20, 93, 330, 380, 630
BseNI ACTGG 2 cut(s) 349, 495
BseSI GKGCMC 1 cut(s) 154
BseX3I CGGCCG 1 cut(s) 956
BseXI GCAGC 1 cut(s) 286
Bsh1285I CGRYCG 1 cut(s) 959
BshFI GGCC 6 cut(s) 111, 152, 188, 221, 556, 958
BshNI GGYRCC 2 cut(s) 127, 177
BsiEI CGRYCG 1 cut(s) 959
BsiHKCI CYCGRG 1 cut(s) 503
BsiSI CCGG 5 cut(s) 112, 131, 166, 752, 959
BslFI GGGAC 2 cut(s) 843, 872
BslI CCNNNNNNNGG 2 cut(s) 217, 474
BsmAI GTCTC 3 cut(s) 9, 75, 918
BsmBI CGTCTC 1 cut(s) 75
BsmFI GGGAC 2 cut(s) 843, 872
BsnI GGCC 6 cut(s) 111, 152, 188, 221, 556, 958
BsoBI CYCGRG 1 cut(s) 503
Bsp120I GGGCCC 1 cut(s) 150
Bsp1286I GDGCHC 2 cut(s) 154, 367
Bsp143I GATC 3 cut(s) 141, 628, 718
Bsp19I CCATGG 1 cut(s) 216
BspACI CCGC 5 cut(s) 13, 125, 148, 264, 361
BspANI GGCC 6 cut(s) 111, 152, 188, 221, 556, 958
BspCNI CTCAG 5 cut(s) 19, 94, 329, 379, 631
BspLI GGNNCC 6 cut(s) 129, 152, 179, 495, 720, 932
BspMAI CTGCAG 1 cut(s) 304
BspPI GGATC 3 cut(s) 636, 713, 726
BspQI GCTCTTC 1 cut(s) 573
BspT107I GGYRCC 2 cut(s) 127, 177
BsrBI CCGCTC 1 cut(s) 148
BsrFI RCCGGY 2 cut(s) 130, 958
BsrI ACTGG 2 cut(s) 349, 495
BssAI RCCGGY 2 cut(s) 130, 958
BssECI CCNNGG 5 cut(s) 189, 190, 216, 372, 750
BssMI GATC 3 cut(s) 141, 628, 718
BssNI GRCGYC 1 cut(s) 128
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 4 cut(s) 182, 191, 374, 695
Bst4CI ACNGT 5 cut(s) 45, 395, 863, 924, 946
Bst6I CTCTTC 5 cut(s) 522, 534, 573, 841, 903
BstACI GRCGYC 1 cut(s) 128
BstC8I GCNNGC 6 cut(s) 132, 150, 268, 363, 636, 810
BstDEI CTNAG 5 cut(s) 6, 102, 316, 366, 639
BstDSI CCRYGG 1 cut(s) 216
BstF5I GGATG 7 cut(s) 55, 120, 440, 550, 669, 809, 877
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 3 cut(s) 144, 631, 721
BstMAI GTCTC 3 cut(s) 9, 75, 918
BstMBI GATC 3 cut(s) 141, 628, 718
BstMCI CGRYCG 1 cut(s) 959
BstMWI GCNNNNNNNGC 3 cut(s) 21, 125, 131
BstNI CCWGG 4 cut(s) 182, 191, 374, 695
BstSCI CCNGG 6 cut(s) 165, 180, 189, 372, 693, 750
BstSFI CTRYAG 1 cut(s) 300
BstSLI GKGCMC 1 cut(s) 154
BstV1I GCAGC 1 cut(s) 286
BstX2I RGATCY 1 cut(s) 718
BstYI RGATCY 1 cut(s) 718
BstZI CGGCCG 1 cut(s) 956
BsuRI GGCC 6 cut(s) 111, 152, 188, 221, 556, 958
BtgI CCRYGG 1 cut(s) 216
BtsCI GGATG 7 cut(s) 55, 120, 440, 550, 669, 809, 877
BtsIMutI CAGTG 4 cut(s) 324, 342, 488, 868
Cac8I GCNNGC 6 cut(s) 132, 150, 268, 363, 636, 810
CaiI CAGNNNCTG 1 cut(s) 373
CfoI GCGC 1 cut(s) 130
Cfr10I RCCGGY 2 cut(s) 130, 958
Cfr13I GGNCC 4 cut(s) 150, 151, 187, 554
CseI GACGC 1 cut(s) 487
Csp6I GTAC 5 cut(s) 163, 178, 312, 391, 608
CviAII CATG 2 cut(s) 160, 217
CviQI GTAC 5 cut(s) 163, 178, 312, 391, 608
DdeI CTNAG 5 cut(s) 6, 102, 316, 366, 639
DinI GGCGCC 1 cut(s) 129
DpnI GATC 3 cut(s) 143, 630, 720
DpnII GATC 3 cut(s) 141, 628, 718
DrdI GACNNNNNNGTC 1 cut(s) 484
DseDI GACNNNNNNGTC 1 cut(s) 484
EaeI YGGCCR 3 cut(s) 109, 219, 956
EagI CGGCCG 1 cut(s) 956
Eam1104I CTCTTC 5 cut(s) 522, 534, 573, 841, 903
EarI CTCTTC 5 cut(s) 522, 534, 573, 841, 903
EclXI CGGCCG 1 cut(s) 956
Eco130I CCWWGG 1 cut(s) 216
Eco24I GRGCYC 2 cut(s) 154, 367
Eco52I CGGCCG 1 cut(s) 956
Eco57I CTGAAG 1 cut(s) 777
Eco88I CYCGRG 1 cut(s) 503
EcoO109I RGGNCCY 1 cut(s) 187
EcoRII CCWGG 4 cut(s) 180, 189, 372, 693
EcoT14I CCWWGG 1 cut(s) 216
EcoT38I GRGCYC 2 cut(s) 154, 367
EgeI GGCGCC 1 cut(s) 129
EheI GGCGCC 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 216
Esp3I CGTCTC 1 cut(s) 75
FaeI CATG 2 cut(s) 163, 220
FaiI YATR 7 cut(s) 161, 218, 425, 567, 588, 590, 849
FaqI GGGAC 2 cut(s) 843, 872
FatI CATG 2 cut(s) 159, 216
FauI CCCGC 2 cut(s) 141, 354
Fnu4HI GCNGC 3 cut(s) 126, 264, 300
FokI GGATG 7 cut(s) 42, 127, 427, 557, 656, 816, 884
FriOI GRGCYC 2 cut(s) 154, 367
Fsp4HI GCNGC 3 cut(s) 126, 264, 300
FspBI CTAG 2 cut(s) 521, 879
GlaI GCGC 1 cut(s) 129
GluI GCNGC 3 cut(s) 126, 264, 300
GsuI CTGGAG 2 cut(s) 4, 366
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 6 cut(s) 111, 152, 188, 221, 556, 958
HapII CCGG 5 cut(s) 112, 131, 166, 752, 959
HgaI GACGC 1 cut(s) 487
HhaI GCGC 1 cut(s) 130
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 2 cut(s) 163, 220
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 5 cut(s) 195, 506, 698, 875, 911
HpaII CCGG 5 cut(s) 112, 131, 166, 752, 959
HphI GGTGA 2 cut(s) 388, 777
Hpy188I TCNGA 5 cut(s) 79, 369, 445, 456, 511
Hpy188III TCNNGA 4 cut(s) 284, 679, 842, 915
HpyAV CCTTC 4 cut(s) 164, 614, 618, 846
HpyCH4III ACNGT 5 cut(s) 45, 395, 863, 924, 946
HpyCH4V TGCA 1 cut(s) 302
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 125, 131
HpyF3I CTNAG 5 cut(s) 6, 102, 316, 366, 639
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 2 cut(s) 163, 220
HspAI GCGC 1 cut(s) 128
KasI GGCGCC 1 cut(s) 127
KpnI GGTACC 1 cut(s) 181
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
Kzo9I GATC 3 cut(s) 141, 628, 718
LguI GCTCTTC 1 cut(s) 573
LmnI GCTCC 3 cut(s) 15, 347, 499
Lsp1109I GCAGC 1 cut(s) 286
LweI GCATC 1 cut(s) 128
MaeI CTAG 2 cut(s) 521, 879
MaeIII GTNAC 4 cut(s) 45, 376, 486, 760
MalI GATC 3 cut(s) 143, 630, 720
MbiI CCGCTC 1 cut(s) 148
MboI GATC 3 cut(s) 141, 628, 718
MflI RGATCY 1 cut(s) 718
MhlI GDGCHC 2 cut(s) 154, 367
MlsI TGGCCA 1 cut(s) 221
MluNI TGGCCA 1 cut(s) 221
Mly113I GGCGCC 1 cut(s) 128
MlyI GAGTC 4 cut(s) 515, 692, 869, 920
Mox20I TGGCCA 1 cut(s) 221
MreI CGCCGGCG 1 cut(s) 130
MroNI GCCGGC 1 cut(s) 130
MscI TGGCCA 1 cut(s) 221
MseI TTAA 5 cut(s) 414, 431, 473, 645, 738
Msp20I TGGCCA 1 cut(s) 221
MspA1I CMGCKG 1 cut(s) 93
MspI CCGG 5 cut(s) 112, 131, 166, 752, 959
MspR9I CCNGG 6 cut(s) 167, 182, 191, 374, 695, 752
MvaI CCWGG 4 cut(s) 182, 191, 374, 695
MwoI GCNNNNNNNGC 3 cut(s) 21, 125, 131
NaeI GCCGGC 1 cut(s) 132
NarI GGCGCC 1 cut(s) 128
NciI CCSGG 2 cut(s) 167, 752
NcoI CCATGG 1 cut(s) 216
NdeII GATC 3 cut(s) 141, 628, 718
NgoMIV GCCGGC 1 cut(s) 130
NlaIII CATG 2 cut(s) 163, 220
NlaIV GGNNCC 6 cut(s) 129, 152, 179, 495, 720, 932
NmuCI GTSAC 3 cut(s) 45, 376, 486
PasI CCCWGGG 1 cut(s) 190
PciSI GCTCTTC 1 cut(s) 573
PdiI GCCGGC 1 cut(s) 132
PfeI GAWTC 1 cut(s) 195
PkrI GCNGC 3 cut(s) 127, 265, 301
PleI GAGTC 4 cut(s) 514, 692, 869, 919
PluTI GGCGCC 1 cut(s) 131
PpsI GAGTC 4 cut(s) 514, 692, 869, 919
Psp6I CCWGG 4 cut(s) 180, 189, 372, 693
PspGI CCWGG 4 cut(s) 180, 189, 372, 693
PspN4I GGNNCC 6 cut(s) 129, 152, 179, 495, 720, 932
PspOMI GGGCCC 1 cut(s) 150
PspPI GGNCC 4 cut(s) 150, 151, 187, 554
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 373
PsuI RGATCY 1 cut(s) 718
PvuII CAGCTG 1 cut(s) 93
RsaI GTAC 5 cut(s) 164, 179, 313, 392, 609
RsaNI GTAC 5 cut(s) 163, 178, 312, 391, 608
SapI GCTCTTC 1 cut(s) 573
SaqAI TTAA 5 cut(s) 414, 431, 473, 645, 738
SatI GCNGC 3 cut(s) 126, 264, 300
Sau3AI GATC 3 cut(s) 141, 628, 718
Sau96I GGNCC 4 cut(s) 150, 151, 187, 554
SchI GAGTC 4 cut(s) 515, 692, 869, 920
ScrFI CCNGG 6 cut(s) 167, 182, 191, 374, 695, 752
SduI GDGCHC 2 cut(s) 154, 367
SfaNI GCATC 1 cut(s) 128
SfcI CTRYAG 1 cut(s) 300
SfoI GGCGCC 1 cut(s) 129
SgrAI CRCCGGYG 1 cut(s) 130
SmlI CTYRAG 3 cut(s) 275, 323, 677
SmoI CTYRAG 3 cut(s) 275, 323, 677
SsiI CCGC 5 cut(s) 13, 125, 148, 264, 361
SspDI GGCGCC 1 cut(s) 127
SspMI CTAG 2 cut(s) 521, 879
StyD4I CCNGG 6 cut(s) 165, 180, 189, 372, 693, 750
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 5 cut(s) 45, 395, 863, 924, 946
TaqI TCGA 2 cut(s) 144, 916
TauI GCSGC 2 cut(s) 128, 266
TfiI GAWTC 1 cut(s) 195
Tru1I TTAA 5 cut(s) 414, 431, 473, 645, 738
Tru9I TTAA 5 cut(s) 414, 431, 473, 645, 738
TscAI CASTG 4 cut(s) 324, 349, 495, 868
TseFI GTSAC 3 cut(s) 45, 376, 486
TseI GCWGC 1 cut(s) 299
Tsp45I GTSAC 3 cut(s) 45, 376, 486
TspDTI ATGAA 1 cut(s) 561
TspGWI ACGGA 2 cut(s) 57, 923
TspRI CASTG 4 cut(s) 324, 349, 495, 868
XspI CTAG 2 cut(s) 521, 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.