Rmu_sc0002978.1_g000002

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002978.1
OG0010840
Physical Location & Seq
Reverse (-)
2559 .. 5468
2910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002978.1_g000002.1.cds

Sequence Viewer

Length: 2700 bp
atggaagaagaagaccagcaaaaagtcgcagatctggtcaaggagctcgtcattcgcttggtcgcccaaaactccacatccgaatctcaccaaccaaatcccaactcccttcatctccaaacctcacttcgctacgcgattcgcttactctccagccgattgactccctccgtcgccccggacgccgccgcaatcgccgagtccaccaaacgccgactcgccactcaaggtaagtcctcgcaagccctaactttcgccgacctttatactaagttcgcatccaaaaccgggcccggaagtgtaaataacaagtgggcggtcatctatttgcttaagattatatcggaggatcgaaagagtgtgaattcccagttggattcttcggttttgttgcccaatttgggtttaaacgacgccggatcgggcgacgagtcgagggttttggggggaaggggaagtggggaaaagggttggagcaatggggttttgttagtttccaaggacccggagaatctccgggaaattgcgtttagggagtttgtgaatttggttagggaagagaatgaagtttccgaagaggctttggtgagagatgtgttgtatgcttgtcagggaattgatgggaagtatgtgaaaacgacgccggatcgggcgacgagtcgaggggagtttgtgaatttggttaaggaagagaatgaagtttccgaagaggctttggtgagagatgtgttgtatgcttgtcagggaattgatgggaagtatgtgaagttcgacagtaatgcggacggctatgttttgtccgattcgataaaggttccgagagctaccaggattatggttcggaagctttgtgaattggggtggttgtttaggaaggtgaaggggtatatctcggagagtatggatgggtttccatccgaagatgttggaaccattgggcaggctttttgtgctgcattgcaagatgagctctccgattattataagctgttggcggtgcttgaagcacaggcgatgaatccgattcccttggtttcagagacggtgagctcggggaattatctttctatgaggaggttgtctgtttggtttgcagagccaatggtgaaaatgaggttgatggctgttttggtagataaatgtagggttttaaggggtggggcaatgactggggctattcatatgcatgctcagcatggggacccactagtgcatgagttcatggggcggttactccgtcgggtgtgctccccactgtttgagatggtcaggagttgggttttggaaggggagttagaagatgtttttgctgaattctttattgtgggtcagccggtgaaagctgaatctctttggagggaaggttacatgctccatgctgggatgcttccttcttttatttcacaatctcttgctcaacgcattctgaggactggaaagtcgattaatttccttcgcgtctgttgtgacgatcgtggttgggcagatgctgcactagaagctgcagctgctgctggcaccacgacaagaagatggggtcttggatatggtgaaactgatgctcttgagtctttggtcgatggagcagcaaagagagttgataagcatttgttggatgttatatacaaccactataagttcaaggagcactgtcttgcaataaagcggtatttactgcttggacagggtgactttgttcagtacctaatggatattgttgggccagagctttctgagcctgctaacactataagctctttccagctagcggggttgctggaaactgcaatccgatcttctaatgcacaatatgatgatcgcgacatattggatagattgaaggtaaagatgatgccacatgaaactggagatagaggttgggatgtattttcattggaatatgatgcaagggttcctttagatactgtttttacggaatccgtgatgacaaagtatttaagaatatttaatttcttgtggaagcttcgacgagtagagcatgctcttattggtgcttggaagactatgaaaccaaattgtatcactgcccgttccttcatgaagctgcagaatgcagttaagttgccgttgctttcaactctgagacgatgccaggttctttgggatgagatgaatcattttgtttcaaacttgcagtactatattatgtttgaagtcttggaggtgtcatggtctgactttttgaacgaaatggaggtggccaaggaccttgatgatctacttgctgcacatgaaaagtacctccattcgattgtggagaaatccctccttggagaacgttcccaaaccctttacagctcactctttgccttgtttgatcttatattgaaatttcgaagtcatgctgatagattgtctgaagggatcaatgagttgcaagcaagaaccatggaatcctcagcaccctctcgagtcaagagtaaaacgaaaaagcgattaaatgatagatcctcagagcctgggtcctggattagtgatggcaggaaggccctcacacaacgcgctggtgaatttcttcggaatatgggacaggatctagatgcagtagcaaaagaatattcgtcattgcttgaggattttatttctaagttgcccatgcagcaacatgttgatttaaagttcctcctgtttcggcttgacttcaccgaattttacagccagttgcgtcctagtacgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000280 GO:0000923 GO:0000930 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0008092 GO:0008150 GO:0008275 GO:0009605 GO:0009607 GO:0009624 GO:0009898 GO:0009987 GO:0010638 GO:0010968 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0022402 GO:0022414 GO:0022607 GO:0030863 GO:0030981 GO:0031109 GO:0031110 GO:0031112 GO:0031113 GO:0031116 GO:0031122 GO:0031334 GO:0031967 GO:0031975 GO:0032153 GO:0032155 GO:0032271 GO:0032273 GO:0032886 GO:0032991 GO:0033043 GO:0034622 GO:0043015 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044450 GO:0044459 GO:0044464 GO:0046785 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051225 GO:0051258 GO:0051321 GO:0051415 GO:0051418 GO:0051493 GO:0051495 GO:0051704 GO:0051707 GO:0055028 GO:0065003 GO:0065007 GO:0070507 GO:0070925 GO:0071840 GO:0071944 GO:0090063 GO:0090307 GO:0097435 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1902850 GO:1902903 GO:1902905 GO:1903047
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spc97_Spc98

Protein Analysis

899

Amino Acids

101.46

Weight (kDa)

6.11

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 984
AasI GACNNNNNNGTC 1 cut(s) 1438
AccB1I GGYRCC 1 cut(s) 1517
AccII CGCG 4 cut(s) 137, 1458, 1821, 2523
AciI CCGC 8 cut(s) 186, 189, 317, 782, 995, 1228, 1666, 1769
AclI AACGTT 1 cut(s) 2299
AclWI GGATC 6 cut(s) 357, 427, 654, 2393, 2463, 2562
AcoI YGGCCR 1 cut(s) 2220
AcsI RAATTY 7 cut(s) 364, 544, 676, 1313, 2351, 2531, 2669
AcuI CTGAAG 1 cut(s) 2400
AcyI GRCGYC 3 cut(s) 183, 414, 641
AfaI GTAC 4 cut(s) 1703, 2159, 2261, 2695
AfiI CCNNNNNNNGG 3 cut(s) 288, 401, 1768
AflII CTTAAG 1 cut(s) 332
AflIII ACRYGT 1 cut(s) 2626
AgsI TTSAA 8 cut(s) 1004, 1642, 1840, 2097, 2148, 2174, 2206, 2350
AhlI ACTAGT 1 cut(s) 1207
AjnI CCWGG 4 cut(s) 827, 2112, 2479, 2486
AjuI GAANNNNNNNTTGG 4 cut(s) 111, 143, 356, 388
Alw21I GWGCWC 5 cut(s) 48, 972, 1052, 1250, 1650
Alw26I GTCTC 2 cut(s) 1034, 2098
AlwI GGATC 6 cut(s) 357, 427, 654, 2393, 2463, 2562
AlwNI CAGNNNCTG 3 cut(s) 1490, 1511, 2480
Ama87I CYCGRG 2 cut(s) 1051, 2430
AoxI GGCC 4 cut(s) 290, 1721, 2220, 2508
ApaI GGGCCC 1 cut(s) 294
ApoI RAATTY 7 cut(s) 364, 544, 676, 1313, 2351, 2531, 2669
AseI ATTAAT 1 cut(s) 1446
Asp700I GAANNNNTTC 1 cut(s) 2535
AspLEI GCGC 1 cut(s) 2525
AspS9I GGNCC 8 cut(s) 290, 291, 502, 1201, 1721, 2227, 2484, 2509
AsuC2I CCSGG 5 cut(s) 179, 289, 294, 506, 518
AsuII TTCGAA 1 cut(s) 2356
AsuNHI GCTAGC 1 cut(s) 1765
AvaI CYCGRG 2 cut(s) 1051, 2430
AvaII GGWCC 4 cut(s) 502, 1201, 2227, 2484
BaeGI GKGCMC 1 cut(s) 294
BalI TGGCCA 1 cut(s) 2222
BanI GGYRCC 1 cut(s) 1517
BanII GRGCYC 4 cut(s) 48, 294, 972, 1052
BbsI GAAGAC 2 cut(s) 18, 2027
Bbv12I GWGCWC 5 cut(s) 48, 972, 1052, 1250, 1650
BbvCI CCTCAGC 1 cut(s) 2419
BccI CCATC 9 cut(s) 614, 746, 899, 922, 1114, 1258, 1527, 1574, 2492
BceAI ACGGC 2 cut(s) 802, 2071
BcgI CGANNNNNNTGC 2 cut(s) 761, 795
BciT130I CCWGG 4 cut(s) 829, 2114, 2481, 2488
BcnI CCSGG 5 cut(s) 179, 289, 294, 506, 518
BcoDI GTCTC 2 cut(s) 1034, 2098
BcuI ACTAGT 1 cut(s) 1207
BfaI CTAG 5 cut(s) 1208, 1496, 1766, 2558, 2691
BfmI CTRYAG 2 cut(s) 1503, 2066
BfrI CTTAAG 1 cut(s) 332
BglII AGATCT 1 cut(s) 31
BlpI GCTNAGC 1 cut(s) 1191
BmcAI AGTACT 1 cut(s) 2159
Bme1390I CCNGG 9 cut(s) 179, 289, 294, 506, 518, 829, 2114, 2481, 2488
Bme18I GGWCC 4 cut(s) 502, 1201, 2227, 2484
BmeT110I CYCGRG 2 cut(s) 1051, 2430
BmgT120I GGNCC 8 cut(s) 290, 291, 502, 1201, 1721, 2227, 2484, 2509
BmiI GGNNCC 9 cut(s) 292, 504, 816, 931, 1202, 1203, 1519, 1914, 2485
BmrFI CCNGG 9 cut(s) 179, 289, 294, 506, 518, 829, 2114, 2481, 2488
BmrI ACTGGG 2 cut(s) 364, 1179
BmsI GCATC 8 cut(s) 287, 1374, 1477, 1549, 1842, 1894, 2099, 2551
BmtI GCTAGC 1 cut(s) 1769
BmuI ACTGGG 2 cut(s) 364, 1179
BpiI GAAGAC 2 cut(s) 18, 2027
BpmI CTGGAG 2 cut(s) 136, 1887
Bpu10I CCTNAGC 1 cut(s) 2419
Bpu1102I GCTNAGC 1 cut(s) 1191
Bpu14I TTCGAA 1 cut(s) 2356
BpuEI CTTGAG 3 cut(s) 210, 1586, 2612
BpuMI CCSGG 5 cut(s) 179, 289, 294, 506, 518
BsaAI YACGTR 1 cut(s) 2697
BsaHI GRCGYC 3 cut(s) 183, 414, 641
BsaJI CCNNGG 7 cut(s) 177, 498, 1029, 2223, 2290, 2409, 2480
BsaXI ACNNNNNCTCC 2 cut(s) 1348, 1378
Bsc4I CCNNNNNNNGG 3 cut(s) 288, 401, 1768
Bse118I RCCGGY 1 cut(s) 1333
Bse1I ACTGG 5 cut(s) 370, 1174, 1438, 1870, 2680
Bse3DI GCAATG 4 cut(s) 484, 956, 1170, 2585
BseBI CCWGG 4 cut(s) 829, 2114, 2481, 2488
BseDI CCNNGG 7 cut(s) 177, 498, 1029, 2223, 2290, 2409, 2480
BseGI GGATG 8 cut(s) 77, 278, 910, 914, 1389, 1621, 1888, 2131
BseLI CCNNNNNNNGG 3 cut(s) 288, 401, 1768
BseMI GCAATG 4 cut(s) 484, 956, 1170, 2585
BseMII CTCAG 6 cut(s) 1205, 1418, 1725, 2093, 2433, 2487
BseNI ACTGG 5 cut(s) 370, 1174, 1438, 1870, 2680
BseRI GAGGAG 1 cut(s) 1087
BseSI GKGCMC 1 cut(s) 294
BseYI CCCAGC 1 cut(s) 1379
BsgI GTGCAG 2 cut(s) 1476, 2232
Bsh1236I CGCG 4 cut(s) 137, 1458, 1821, 2523
Bsh1285I CGRYCG 1 cut(s) 1474
BshFI GGCC 4 cut(s) 292, 1723, 2222, 2510
BshNI GGYRCC 1 cut(s) 1517
BsiEI CGRYCG 1 cut(s) 1474
BsiHKAI GWGCWC 5 cut(s) 48, 972, 1052, 1250, 1650
BsiHKCI CYCGRG 2 cut(s) 1051, 2430
BsiSI CCGG 8 cut(s) 179, 288, 294, 417, 506, 517, 644, 1334
BslFI GGGAC 2 cut(s) 1214, 2562
BslI CCNNNNNNNGG 3 cut(s) 288, 401, 1768
BsmAI GTCTC 2 cut(s) 1034, 2098
BsmBI CGTCTC 2 cut(s) 1034, 2098
BsmFI GGGAC 2 cut(s) 1214, 2562
BsmI GAATGC 2 cut(s) 1422, 2077
BsnI GGCC 4 cut(s) 292, 1723, 2222, 2510
BsoBI CYCGRG 2 cut(s) 1051, 2430
Bsp119I TTCGAA 1 cut(s) 2356
Bsp120I GGGCCC 1 cut(s) 290
Bsp1286I GDGCHC 6 cut(s) 48, 294, 972, 1052, 1250, 1650
Bsp1720I GCTNAGC 1 cut(s) 1191
Bsp19I CCATGG 1 cut(s) 2409
Bsp68I TCGCGA 1 cut(s) 1821
BspACI CCGC 8 cut(s) 186, 189, 317, 782, 995, 1228, 1666, 1769
BspANI GGCC 4 cut(s) 292, 1723, 2222, 2510
BspCNI CTCAG 6 cut(s) 1204, 1419, 1726, 2094, 2432, 2486
BspFNI CGCG 4 cut(s) 137, 1458, 1821, 2523
BspHI TCATGA 1 cut(s) 2058
BspLI GGNNCC 9 cut(s) 292, 504, 816, 931, 1202, 1203, 1519, 1914, 2485
BspMAI CTGCAG 2 cut(s) 1507, 2070
BspOI GCTAGC 1 cut(s) 1769
BspPI GGATC 6 cut(s) 357, 427, 654, 2393, 2463, 2562
BspT104I TTCGAA 1 cut(s) 2356
BspT107I GGYRCC 1 cut(s) 1517
BspTI CTTAAG 1 cut(s) 332
BsrDI GCAATG 4 cut(s) 484, 956, 1170, 2585
BsrFI RCCGGY 1 cut(s) 1333
BsrI ACTGG 5 cut(s) 370, 1174, 1438, 1870, 2680
BssAI RCCGGY 1 cut(s) 1333
BssECI CCNNGG 7 cut(s) 177, 498, 1029, 2223, 2290, 2409, 2480
BssNI GRCGYC 3 cut(s) 183, 414, 641
BssT1I CCWWGG 5 cut(s) 498, 1029, 2223, 2290, 2409
Bst2UI CCWGG 4 cut(s) 829, 2114, 2481, 2488
Bst4CI ACNGT 5 cut(s) 776, 1045, 1257, 1652, 1927
Bst6I CTCTTC 4 cut(s) 552, 570, 684, 702
BstACI GRCGYC 3 cut(s) 183, 414, 641
BstAFI CTTAAG 1 cut(s) 332
BstAPI GCANNNNNTGC 2 cut(s) 1490, 1511
BstBAI YACGTR 1 cut(s) 2697
BstBI TTCGAA 1 cut(s) 2356
BstC8I GCNNGC 8 cut(s) 243, 942, 1188, 1516, 1740, 1767, 2001, 2400
BstDEI CTNAG 8 cut(s) 270, 1191, 1427, 1734, 2102, 2419, 2473, 2607
BstDSI CCRYGG 1 cut(s) 2409
BstF5I GGATG 8 cut(s) 77, 278, 910, 914, 1389, 1621, 1888, 2131
BstFNI CGCG 4 cut(s) 137, 1458, 1821, 2523
BstHHI GCGC 1 cut(s) 2525
BstMAI GTCTC 2 cut(s) 1034, 2098
BstMCI CGRYCG 1 cut(s) 1474
BstNI CCWGG 4 cut(s) 829, 2114, 2481, 2488
BstNSI RCATGY 4 cut(s) 1190, 1372, 2003, 2630
BstSCI CCNGG 9 cut(s) 177, 287, 292, 504, 516, 827, 2112, 2479, 2486
BstSFI CTRYAG 2 cut(s) 1503, 2066
BstSLI GKGCMC 1 cut(s) 294
BstSNI TACGTA 1 cut(s) 2697
BstUI CGCG 4 cut(s) 137, 1458, 1821, 2523
BstV2I GAAGAC 2 cut(s) 18, 2027
BstX2I RGATCY 3 cut(s) 31, 2468, 2554
BstXI CCANNNNNNTGG 1 cut(s) 835
BstYI RGATCY 3 cut(s) 31, 2468, 2554
BsuRI GGCC 4 cut(s) 292, 1723, 2222, 2510
BtgI CCRYGG 1 cut(s) 2409
BtgZI GCGATG 1 cut(s) 1028
BtsCI GGATG 8 cut(s) 77, 278, 910, 914, 1389, 1621, 1888, 2131
BtsI GCAGTG 1 cut(s) 2043
BtsIMutI CAGTG 3 cut(s) 1253, 1648, 2043
BtuMI TCGCGA 1 cut(s) 1821
Cac8I GCNNGC 8 cut(s) 243, 942, 1188, 1516, 1740, 1767, 2001, 2400
CaiI CAGNNNCTG 3 cut(s) 1490, 1511, 2480
CciI TCATGA 1 cut(s) 2058
CfoI GCGC 1 cut(s) 2525
Cfr10I RCCGGY 1 cut(s) 1333
Cfr13I GGNCC 8 cut(s) 290, 291, 502, 1201, 1721, 2227, 2484, 2509
CseI GACGC 5 cut(s) 191, 422, 649, 1447, 2675
Csp6I GTAC 4 cut(s) 1702, 2158, 2260, 2694
CviQI GTAC 4 cut(s) 1702, 2158, 2260, 2694
DdeI CTNAG 8 cut(s) 270, 1191, 1427, 1734, 2102, 2419, 2473, 2607
DraI TTTAAA 2 cut(s) 408, 2637
DrdI GACNNNNNNGTC 1 cut(s) 1438
DseDI GACNNNNNNGTC 1 cut(s) 1438
EaeI YGGCCR 1 cut(s) 2220
Eam1104I CTCTTC 4 cut(s) 552, 570, 684, 702
EarI CTCTTC 4 cut(s) 552, 570, 684, 702
Ecl136II GAGCTC 3 cut(s) 46, 970, 1050
Eco105I TACGTA 1 cut(s) 2697
Eco130I CCWWGG 5 cut(s) 498, 1029, 2223, 2290, 2409
Eco24I GRGCYC 4 cut(s) 48, 294, 972, 1052
Eco47I GGWCC 4 cut(s) 502, 1201, 2227, 2484
Eco53kI GAGCTC 3 cut(s) 46, 970, 1050
Eco57I CTGAAG 1 cut(s) 2400
Eco88I CYCGRG 2 cut(s) 1051, 2430
EcoICRI GAGCTC 3 cut(s) 46, 970, 1050
EcoO109I RGGNCCY 5 cut(s) 502, 1201, 2227, 2484, 2509
EcoRI GAATTC 2 cut(s) 364, 1313
EcoRII CCWGG 4 cut(s) 827, 2112, 2479, 2486
EcoT14I CCWWGG 5 cut(s) 498, 1029, 2223, 2290, 2409
EcoT22I ATGCAT 1 cut(s) 1188
EcoT38I GRGCYC 4 cut(s) 48, 294, 972, 1052
ErhI CCWWGG 5 cut(s) 498, 1029, 2223, 2290, 2409
Esp3I CGTCTC 2 cut(s) 1034, 2098
FalI AAGNNNNNCTT 2 cut(s) 1900, 1932
FaqI GGGAC 2 cut(s) 1214, 2562
FauI CCCGC 1 cut(s) 1762
FauNDI CATATG 1 cut(s) 1182
FokI GGATG 8 cut(s) 64, 265, 901, 917, 1396, 1628, 1895, 2138
FriOI GRGCYC 4 cut(s) 48, 294, 972, 1052
FspBI CTAG 5 cut(s) 1208, 1496, 1766, 2558, 2691
GlaI GCGC 1 cut(s) 2524
GsaI CCCAGC 1 cut(s) 1383
GsuI CTGGAG 2 cut(s) 136, 1887
HaeIII GGCC 4 cut(s) 292, 1723, 2222, 2510
HapII CCGG 8 cut(s) 179, 288, 294, 417, 506, 517, 644, 1334
HgaI GACGC 5 cut(s) 191, 422, 649, 1447, 2675
HhaI GCGC 1 cut(s) 2525
Hin1I GRCGYC 3 cut(s) 183, 414, 641
Hin6I GCGC 1 cut(s) 2523
HinP1I GCGC 1 cut(s) 2523
HindIII AAGCTT 2 cut(s) 845, 1982
HpaII CCGG 8 cut(s) 179, 288, 294, 417, 506, 517, 644, 1334
Hpy166II GTNNAC 1 cut(s) 204
Hpy188III TCNNGA 7 cut(s) 1270, 1565, 1820, 2059, 2430, 2437, 2558
Hpy8I GTNNAC 1 cut(s) 204
Hpy99I CGWCG 7 cut(s) 176, 416, 431, 643, 658, 1242, 1992
HpyCH4III ACNGT 5 cut(s) 776, 1045, 1257, 1652, 1927
HpyCH4IV ACGT 2 cut(s) 2299, 2696
HpyF3I CTNAG 8 cut(s) 270, 1191, 1427, 1734, 2102, 2419, 2473, 2607
HpySE526I ACGT 2 cut(s) 2299, 2696
Hsp92I GRCGYC 3 cut(s) 183, 414, 641
HspAI GCGC 1 cut(s) 2523
KflI GGGWCCC 1 cut(s) 1201
LmnI GCTCC 6 cut(s) 43, 474, 1253, 1377, 1583, 1645
LweI GCATC 8 cut(s) 287, 1374, 1477, 1549, 1842, 1894, 2099, 2551
MaeI CTAG 5 cut(s) 1208, 1496, 1766, 2558, 2691
MaeII ACGT 2 cut(s) 2299, 2696
MaeIII GTNAC 4 cut(s) 1230, 1364, 1466, 1688
MflI RGATCY 3 cut(s) 31, 2468, 2554
MhlI GDGCHC 6 cut(s) 48, 294, 972, 1052, 1250, 1650
MlsI TGGCCA 1 cut(s) 2222
MluNI TGGCCA 1 cut(s) 2222
MlyI GAGTC 7 cut(s) 157, 209, 210, 440, 667, 1577, 2442
MmeI TCCRAC 4 cut(s) 354, 452, 907, 1593
Mox20I TGGCCA 1 cut(s) 2222
Mph1103I ATGCAT 1 cut(s) 1188
MroXI GAANNNNTTC 1 cut(s) 2535
MscI TGGCCA 1 cut(s) 2222
MslI CAYNNNNRTG 1 cut(s) 1185
Msp20I TGGCCA 1 cut(s) 2222
MspA1I CMGCKG 1 cut(s) 1508
MspCI CTTAAG 1 cut(s) 332
MspI CCGG 8 cut(s) 179, 288, 294, 417, 506, 517, 644, 1334
MspR9I CCNGG 9 cut(s) 179, 289, 294, 506, 518, 829, 2114, 2481, 2488
MssI GTTTAAAC 1 cut(s) 408
Mva1269I GAATGC 2 cut(s) 1422, 2077
MvaI CCWGG 4 cut(s) 829, 2114, 2481, 2488
MvnI CGCG 4 cut(s) 137, 1458, 1821, 2523
NciI CCSGG 5 cut(s) 179, 289, 294, 506, 518
NcoI CCATGG 1 cut(s) 2409
NdeI CATATG 1 cut(s) 1182
NheI GCTAGC 1 cut(s) 1765
NlaIV GGNNCC 9 cut(s) 292, 504, 816, 931, 1202, 1203, 1519, 1914, 2485
NmeAIII GCCGAG 1 cut(s) 223
NmuCI GTSAC 2 cut(s) 1466, 1688
NruI TCGCGA 1 cut(s) 1821
NsiI ATGCAT 1 cut(s) 1188
NspI RCATGY 4 cut(s) 1190, 1372, 2003, 2630
NspV TTCGAA 1 cut(s) 2356
PaeI GCATGC 2 cut(s) 1190, 2003
PaeR7I CTCGAG 1 cut(s) 2430
PagI TCATGA 1 cut(s) 2058
PciI ACATGT 1 cut(s) 2626
PctI GAATGC 2 cut(s) 1422, 2077
PdmI GAANNNNTTC 1 cut(s) 2535
PfoI TCCNGGA 2 cut(s) 516, 2486
Ple19I CGATCG 1 cut(s) 1474
PleI GAGTC 7 cut(s) 157, 208, 210, 439, 666, 1576, 2441
PmeI GTTTAAAC 1 cut(s) 408
PpsI GAGTC 7 cut(s) 157, 208, 210, 439, 666, 1576, 2441
Ppu21I YACGTR 1 cut(s) 2697
PpuMI RGGWCCY 4 cut(s) 502, 1201, 2227, 2484
PscI ACATGT 1 cut(s) 2626
PshBI ATTAAT 1 cut(s) 1446
PsiI TTATAA 1 cut(s) 984
Psp124BI GAGCTC 3 cut(s) 48, 972, 1052
Psp1406I AACGTT 1 cut(s) 2299
Psp5II RGGWCCY 4 cut(s) 502, 1201, 2227, 2484
Psp6I CCWGG 4 cut(s) 827, 2112, 2479, 2486
PspFI CCCAGC 1 cut(s) 1379
PspGI CCWGG 4 cut(s) 827, 2112, 2479, 2486
PspN4I GGNNCC 9 cut(s) 292, 504, 816, 931, 1202, 1203, 1519, 1914, 2485
PspOMI GGGCCC 1 cut(s) 290
PspPI GGNCC 8 cut(s) 290, 291, 502, 1201, 1721, 2227, 2484, 2509
PspPPI RGGWCCY 4 cut(s) 502, 1201, 2227, 2484
PsrI GAACNNNNNNTAC 2 cut(s) 752, 784
PstI CTGCAG 2 cut(s) 1507, 2070
PstNI CAGNNNCTG 3 cut(s) 1490, 1511, 2480
PsuI RGATCY 3 cut(s) 31, 2468, 2554
PvuI CGATCG 1 cut(s) 1474
PvuII CAGCTG 1 cut(s) 1508
RruI TCGCGA 1 cut(s) 1821
RsaI GTAC 4 cut(s) 1703, 2159, 2261, 2695
RsaNI GTAC 4 cut(s) 1702, 2158, 2260, 2694
RseI CAYNNNNRTG 1 cut(s) 1185
SacI GAGCTC 3 cut(s) 48, 972, 1052
Sau96I GGNCC 8 cut(s) 290, 291, 502, 1201, 1721, 2227, 2484, 2509
ScaI AGTACT 1 cut(s) 2159
SchI GAGTC 7 cut(s) 157, 209, 210, 440, 667, 1577, 2442
ScrFI CCNGG 9 cut(s) 179, 289, 294, 506, 518, 829, 2114, 2481, 2488
SduI GDGCHC 6 cut(s) 48, 294, 972, 1052, 1250, 1650
SfaNI GCATC 8 cut(s) 287, 1374, 1477, 1549, 1842, 1894, 2099, 2551
SfcI CTRYAG 2 cut(s) 1503, 2066
Sfr274I CTCGAG 1 cut(s) 2430
SfuI TTCGAA 1 cut(s) 2356
SinI GGWCC 4 cut(s) 502, 1201, 2227, 2484
SlaI CTCGAG 1 cut(s) 2430
SmiMI CAYNNNNRTG 1 cut(s) 1185
SmlI CTYRAG 5 cut(s) 225, 332, 1565, 2430, 2591
SmoI CTYRAG 5 cut(s) 225, 332, 1565, 2430, 2591
SnaBI TACGTA 1 cut(s) 2697
SpeI ACTAGT 1 cut(s) 1207
SphI GCATGC 2 cut(s) 1190, 2003
SsiI CCGC 8 cut(s) 186, 189, 317, 782, 995, 1228, 1666, 1769
SspI AATATT 2 cut(s) 1965, 2579
SspMI CTAG 5 cut(s) 1208, 1496, 1766, 2558, 2691
SstI GAGCTC 3 cut(s) 48, 972, 1052
StyD4I CCNGG 9 cut(s) 177, 287, 292, 504, 516, 827, 2112, 2479, 2486
StyI CCWWGG 5 cut(s) 498, 1029, 2223, 2290, 2409
TaaI ACNGT 5 cut(s) 776, 1045, 1257, 1652, 1927
TaiI ACGT 2 cut(s) 2302, 2699
TatI WGTACW 1 cut(s) 2157
TauI GCSGC 2 cut(s) 188, 191
TscAI CASTG 3 cut(s) 1260, 1655, 2050
TseFI GTSAC 2 cut(s) 1466, 1688
Tsp45I GTSAC 2 cut(s) 1466, 1688
TspGWI ACGGA 4 cut(s) 160, 1226, 1930, 1949
TspRI CASTG 3 cut(s) 1260, 1655, 2050
Vha464I CTTAAG 1 cut(s) 332
VpaK11BI GGWCC 4 cut(s) 502, 1201, 2227, 2484
VspI ATTAAT 1 cut(s) 1446
XapI RAATTY 7 cut(s) 364, 544, 676, 1313, 2351, 2531, 2669
XbaI TCTAGA 1 cut(s) 2557
XceI RCATGY 4 cut(s) 1190, 1372, 2003, 2630
XhoI CTCGAG 1 cut(s) 2430
XmnI GAANNNNTTC 1 cut(s) 2535
XspI CTAG 5 cut(s) 1208, 1496, 1766, 2558, 2691
ZrmI AGTACT 1 cut(s) 2159
Zsp2I ATGCAT 1 cut(s) 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.