Rorug03G0173800

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0010840
Physical Location & Seq
Forward (+)
14692179 .. 14699269
7091 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0173800.1

Sequence Viewer

Length: 2838 bp
ATGGTGAAGTCGTACCTCCGTTACGAGCAGGCGGCGGTCTTCGGCGTCATTTCCTCCGGCGATTCAAACATCACCTACGACAGCTCCGGCAAGCACCTTCTTGCTCCCGCCCTCGAAAAGGTTGGCGTTTGGCACGTACGACAGGGCGTTTGTACCAAAACCCTCACCCCTTCTTCCGCCACCGCCGGAAAACCCATCTCTGTCACTTCCATCGCTTCCTCTCATTCTTCTCTGATAGCTGCTGGGTATTCAGATGGTAGTATAAGAATTTGGGATTCTGAGAAAGGGACCTGTGAGACGACACTCAATGGACATAAAAAGGCTGTCACTGCCTTACGGTATGACAAGCTTGGATCCAAGCTTGCTTCTGGAAGCAAAGATGACGACATTATATTATGGGATGTGGTTGGTGAGAGCGGCCTATATCGCCTCCGTGGGCATCGTGATCAGGTTACGGATCTTGTCTTCTTGGATTCTGGAAAGAAACTCGTTAGTTCCTCAAAAGATAAATTTCTGAAAGTATGGGATCTTGATACCCAGCATTGCATGCAGACAATTAGTGGCCACCATAGTGAAATATGGTCTATAGATAGTGATCCAGAAGAAAGATATTTAGTCTCTGGGTCTGCAGACTCGGAGCTTAGATTTTACACAATTAAGCATGACTCGGAAGATGGCCAATCCATCTCTAATGGGAATGGAACTGTAACTGCGGGGAGTGGAGATCTACCGGTTCATACTAAATGGGAAGTTTTGAAGATGTTTGGTGAGTTACCACGGAAAAACGAGGGTAGAGTCGCAACTGTGAGATTCAACAAGTCCGGAAATTTGCTGGCTTGTCAGGTGGCGGGAAAGACAGTAGATATATTCCATGTACTGGATGAGGATGAATCAAAGCGGAAAGCAAAACGCAGGCTTCACCGGAAGAAAGAAAAAAAATCTGCCAAGGGGGCACCGCAGGTGTTGGAAAATGGAGACACAGATCGTGTAGTTGGAGAAGAAGGAAATAGTCCTGCTGTTACAGTCCTTGATGTTTTTAAGCTTCTTCAAACTATTCGAGCGAGCAAAAAGATATGTTCCATCTCGTTCTGTCCAAACACTACAAAGAGTTCGATGGCTACACTAGCTTTGTCATTGAACAATAATTTGTTGGAATTTTATTCTATCGAAAGCAATGCAACGAACAAAACACTTTCTGTAGAGCTTCAAGGACACCGATCTGATGTCAGAAGTGTAGCACTTAGCTCAGACAACACACTCTTGATGTCAACTAGTCACAATGCAGTAAAGATTTGGAATCCAAGTACTGGTTCCTGCCTGCGAACTATTGATTCCGGTTATGGCATGTGTGGTTTTATCATTCCTAACAGCGAGTATGCACTTGTTGGAACTAAAAGTGGAACAATGGAAATTATTGACATTGGAAGTGGCACTTGCATTGAAGCAGTGGAAGCACATGGTGGCTCTGTCATGTCAATTGCTGCCATTCCTAATGAAAATGGTTTTGTCACAGGAAGTGCAGATCATGATGTTAAGTTTTGGGAATATGAAGTTAAACAGAAGTCTGCTCAAGATTCCAAGCAGCTAGTGGTGTCAAATGTAAGGACTATGAAGATGAACGACGATGCCCTTGTGGTTGCTGTCAGCCCTGATGCCAAATATATCTTGGTTGCACTATTGGATTGCACAGTGAAGGTGTTCTTTATGGATTCACTTAAGCTTTTCCTTTCCTTGTATGGTCATAAGTTGCCTGTATTATGTATGGATACATCATCTGACGGAGACCTAATAGTGACTGGATCTGCTGACAAAAATTTGAAGATTTGGGGTTTAGATTTTGGTGATTGTCATAAATCCTTATTTGCTCATGAGGACAGTGTAACAGCTGTTCGATTTGTGCAAAACACACATTACATGTTTAGTGCTGGAAAGGACCGCATTGTAAAATATTGGGATGCTGATAAATTTCAGTTGCTTTTAACGCTTGAGGGACACCATGCTGAAGTTTGGTGCCTCGGAATTAGTAACCGTGGGGACTTCATTGTCACGGGATCTCATGACAGATCAATCCGTCGCTGGGATCGCACTGAAGAGCCATTTTTTATTGAGGAAGAAGAAGAAAAAAGGCAGTTGGAAATGATTGAGTCTGAACTTGACAATATGATAGAGAGCAGGTATCAAAAGGAAGAAATCCCTGAGGTGGGAGCTGTGGCCTTAGCAGGGAAGAAATCTAAAGAAACAATTACAGCTGCTGATGCAATTATCGATGCAATAGATATGGCAGACGCAGAACTGAAGCGACTTGCGGAACATGAGGAGGAGAAAAGCAGGGGAAGTGTTGCTGACTTTAAACCAAATAGTCTCATGAATGGGCTCTCTCCATCTGAATATGTTCTTCGCGCATTTTCAAATGTTCAGACAAATGATCTTGAGCAGACGCTACTGGCTTTACCGTTCTCAGATGCTTTGAAGCTTATGTCTTACTTGAAGGACTGGACTTTGAATCCTGATAAGGTTGAACTCGTTTGCAAGATTTCTTTAGTTCTATTGCAGACACATTTTAATCAGTTGGTTAGTACACCGGCAGCAAGACCAATGTTAACTGCTTTGAAAGAGATTCTTTATCCCGAAGTTAAGGAACGGAAAGACATCATTGGTTTCAACCTTGCAGGGTTGGAACATCTTAAGCAACTAATGGCTTCGAGATCAGATGCATTATTTCAAGATGCAAAATCAAAGTTGCAGGTAATTCGTGCACAGCATTCCAAGCGCCTAGATGCAAGGACAGACACAGGCAGAGAAGAAAATCGAAGTAAGAAGAGAGACAAAAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000280 GO:0000923 GO:0000930 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0008092 GO:0008150 GO:0008275 GO:0009605 GO:0009607 GO:0009624 GO:0009898 GO:0009987 GO:0010638 GO:0010968 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0022402 GO:0022414 GO:0022607 GO:0030863 GO:0030981 GO:0031109 GO:0031110 GO:0031112 GO:0031113 GO:0031116 GO:0031122 GO:0031334 GO:0031967 GO:0031975 GO:0032153 GO:0032155 GO:0032271 GO:0032273 GO:0032886 GO:0032991 GO:0033043 GO:0034622 GO:0043015 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044450 GO:0044459 GO:0044464 GO:0046785 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051225 GO:0051258 GO:0051321 GO:0051415 GO:0051418 GO:0051493 GO:0051495 GO:0051704 GO:0051707 GO:0055028 GO:0065003 GO:0065007 GO:0070507 GO:0070925 GO:0071840 GO:0071944 GO:0090063 GO:0090307 GO:0097435 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1902850 GO:1902903 GO:1902905 GO:1903047
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spc97_Spc98

Protein Analysis

945

Amino Acids

104.55

Weight (kDa)

6.23

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR75_1st PF23869 10 - 136 4.2e-06 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR5 PF25175 23 - 221 2e-33 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 24 - 218 3.2e-29 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 27 - 222 2.1e-24 CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_2nd PF24782 27 - 134 1.7e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_1st PF25173 31 - 219 1.5e-74 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 31 - 217 7.2e-14 WDR36/Utp21 first beta-propeller
Beta-prop_EML PF23409 40 - 135 9.1e-08 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 41 - 181 2.4e-11 WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 42 - 217 6.8e-15 TEP-1 second beta-propeller
Beta-prop_EML_2 PF23414 61 - 181 9.3e-13 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 61 - 137 4.2e-06 G protein beta WD-40 repeat protein
Beta-prop_SCAP PF24017 61 - 180 2.5e-14 SCAP Beta-propeller
Beta-prop_WDR3_2nd PF25172 66 - 176 6.5e-15 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 67 - 136 2e-09 WDHD1 first WD40 domain
WDR90_beta-prop_4th PF23342 69 - 128 1.2e-06 WDR90, 4th beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 75 - 221 1.1e-13 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 75 - 228 3.1e-15 WDR55
Beta-prop_IFT140_1st PF23383 76 - 135 7.8e-06 IFT140 first beta-propeller
WD40_Prp19 PF24814 77 - 134 2.2e-09 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 78 - 221 1.1e-14 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 96 - 134 7e-07 WD domain, G-beta repeat
EIF3I PF24805 101 - 185 5e-08 EIF3I
Beta-prop_Aladin PF25460 106 - 231 4.7e-06 Aladin seven-bladed propeller
WD40_Prp19 PF24814 119 - 223 3.1e-18 Prp19 WD40 domain
WD40_Gbeta PF25391 123 - 224 1.9e-09 G protein beta WD-40 repeat protein
WD40 PF00400 141 - 176 1e-06 WD domain, G-beta repeat
EIF3I PF24805 144 - 221 1.7e-10 EIF3I
WD40_WDHD1_1st PF24817 150 - 224 3.7e-10 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 172 - 228 1.3e-07 WDR3 second beta-propeller domain
WD40 PF00400 181 - 217 3.9e-06 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 262 - 514 1.3e-15 CDC20/Fizzy WD40 domain
WDR55 PF24796 351 - 524 2.7e-07 WDR55
Beta-prop_WDR5 PF25175 355 - 561 1.5e-16 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 357 - 515 7.8e-15 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 375 - 618 5.2e-25 THOC3 beta-propeller domain
WD40_Gbeta PF25391 377 - 609 1.7e-14 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 377 - 515 4.8e-06 WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 401 - 651 2e-11 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd PF25172 408 - 699 6.3e-136 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 455 - 693 1.7e-21 CDC20/Fizzy WD40 domain
PEP5_VPS11_N PF23341 455 - 718 4.8e-07 PEP5/VPS11 N-terminal
WD40_WDHD1_1st PF24817 456 - 571 4.9e-06 WDHD1 first WD40 domain
WD40_Prp19 PF24814 457 - 653 7.7e-23 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 458 - 639 8.9e-12 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 492 - 657 2e-13 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 499 - 696 1.4e-15 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 533 - 694 4.6e-23 THOC3 beta-propeller domain
WDR55 PF24796 537 - 711 8.7e-11 WDR55
Beta-prop_WDR3_1st PF25173 539 - 694 1.5e-31 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 540 - 693 7.4e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 545 - 696 4.4e-29 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 555 - 693 2.5e-06 WDR36/Utp21 first beta-propeller
WD40 PF00400 576 - 609 1e-07 WD domain, G-beta repeat
Beta-prop_EML PF23409 584 - 655 5.2e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 597 - 694 7.9e-07 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 614 - 652 7.9e-06 WD domain, G-beta repeat
EIF3I PF24805 620 - 697 3e-10 EIF3I
WD40_Prp19 PF24814 641 - 800 2.5e-08 Prp19 WD40 domain
WD40 PF00400 657 - 694 2.8e-08 WD domain, G-beta repeat
Utp12 PF04003 795 - 897 4.5e-23 Dip2/Utp12 Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 949
AasI GACNNNNNNGTC 1 cut(s) 2042
Acc36I ACCTGC 3 cut(s) 949, 2163, 2734
AccB1I GGYRCC 2 cut(s) 952, 2010
AccB7I CCANNNNNTGG 2 cut(s) 877, 1307
AccBSI CCGCTC 1 cut(s) 417
AccII CGCG 1 cut(s) 2400
AccIII TCCGGA 1 cut(s) 821
AclWI GGATC 8 cut(s) 348, 361, 465, 534, 590, 1807, 2059, 2088
AcoI YGGCCR 2 cut(s) 562, 676
AcsI RAATTY 6 cut(s) 267, 509, 826, 1154, 1813, 1964
AcuI CTGAAG 3 cut(s) 2022, 2109, 2315
AcyI GRCGYC 1 cut(s) 45
AdeI CACNNNGTG 1 cut(s) 1460
AfaI GTAC 6 cut(s) 14, 138, 154, 876, 1306, 2578
AfiI CCNNNNNNNGG 7 cut(s) 118, 877, 1307, 2077, 2200, 2201, 2220
AflII CTTAAG 2 cut(s) 1715, 2684
AflIII ACRYGT 1 cut(s) 1914
AgeI ACCGGT 1 cut(s) 730
AhlI ACTAGT 1 cut(s) 1271
AjuI GAANNNNNNNTTGG 2 cut(s) 1294, 1326
AleI CACNNNNGTG 1 cut(s) 570
Alw21I GWGCWC 1 cut(s) 2758
Alw26I GTCTC 6 cut(s) 290, 622, 969, 1776, 2366, 2817
Alw44I GTGCAC 1 cut(s) 2754
AlwI GGATC 8 cut(s) 348, 361, 465, 534, 590, 1807, 2059, 2088
AlwNI CAGNNNCTG 1 cut(s) 2252
Aor13HI TCCGGA 1 cut(s) 821
AoxI GGCC 4 cut(s) 418, 562, 676, 2211
ApaLI GTGCAC 1 cut(s) 2754
ApeKI GCWGC 5 cut(s) 239, 1481, 1582, 2249, 2585
ApoI RAATTY 6 cut(s) 267, 509, 826, 1154, 1813, 1964
ArsI GACNNNNNNTTYG 2 cut(s) 132, 164
AsiGI ACCGGT 1 cut(s) 730
Asp700I GAANNNNTTC 2 cut(s) 1697, 2391
AspLEI GCGC 2 cut(s) 2402, 2772
AspS9I GGNCC 2 cut(s) 288, 1933
AsuHPI GGTGA 7 cut(s) 16, 64, 157, 422, 779, 911, 1853
AvaII GGWCC 2 cut(s) 288, 1933
AxyI CCTNAGG 1 cut(s) 2196
BaeGI GKGCMC 2 cut(s) 955, 2758
BalI TGGCCA 2 cut(s) 564, 678
BamHI GGATCC 1 cut(s) 353
BanI GGYRCC 2 cut(s) 952, 2010
BanII GRGCYC 1 cut(s) 2376
BbsI GAAGAC 2 cut(s) 31, 457
Bbv12I GWGCWC 1 cut(s) 2758
BbvI GCAGC 5 cut(s) 226, 1468, 1594, 2236, 2597
BccI CCATC 8 cut(s) 203, 218, 248, 668, 692, 1088, 1108, 2389
BcgI CGANNNNNNTGC 4 cut(s) 1157, 1191, 2732, 2766
BciVI GTATCC 1 cut(s) 1759
BclI TGATCA 1 cut(s) 445
BcoDI GTCTC 6 cut(s) 290, 622, 969, 1776, 2366, 2817
BcuI ACTAGT 1 cut(s) 1271
BfaI CTAG 4 cut(s) 1124, 1272, 1586, 2774
BfmI CTRYAG 3 cut(s) 585, 627, 1197
BfoI RGCGCY 1 cut(s) 2773
BfrI CTTAAG 2 cut(s) 1715, 2684
BfuAI ACCTGC 3 cut(s) 949, 2163, 2734
BfuI GTATCC 1 cut(s) 1759
BglI GCCNNNNNGGC 1 cut(s) 950
BglII AGATCT 1 cut(s) 724
BisI GCNGC 7 cut(s) 33, 240, 418, 1482, 1583, 2250, 2586
BlsI GCNGC 7 cut(s) 34, 241, 419, 1483, 1584, 2251, 2587
BmcAI AGTACT 1 cut(s) 1306
Bme18I GGWCC 2 cut(s) 288, 1933
BmgT120I GGNCC 2 cut(s) 288, 1933
BmiI GGNNCC 5 cut(s) 289, 355, 954, 1312, 2012
BpiI GAAGAC 2 cut(s) 31, 457
Bpu10I CCTNAGC 1 cut(s) 2215
BpuEI CTTGAG 3 cut(s) 1554, 2006, 2450
Bsa29I ATCGAT 1 cut(s) 2265
BsaAI YACGTR 1 cut(s) 136
BsaBI GATNNNNATC 1 cut(s) 594
BsaHI GRCGYC 1 cut(s) 45
BsaI GGTCTC 1 cut(s) 1776
BsaJI CCNNGG 5 cut(s) 433, 776, 945, 2014, 2029
BsaWI WCCGGW 4 cut(s) 730, 821, 921, 1334
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 7 cut(s) 118, 877, 1307, 2077, 2200, 2201, 2220
Bse118I RCCGGY 2 cut(s) 730, 2581
Bse1I ACTGG 5 cut(s) 882, 1312, 1801, 2448, 2498
Bse21I CCTNAGG 1 cut(s) 2196
Bse3DI GCAATG 2 cut(s) 541, 1180
Bse8I GATNNNNATC 1 cut(s) 594
BseAI TCCGGA 1 cut(s) 821
BseCI ATCGAT 1 cut(s) 2265
BseDI CCNNGG 5 cut(s) 433, 776, 945, 2014, 2029
BseGI GGATG 4 cut(s) 406, 886, 892, 1960
BseJI GATNNNNATC 1 cut(s) 594
BseLI CCNNNNNNNGG 7 cut(s) 118, 877, 1307, 2077, 2200, 2201, 2220
BseMI GCAATG 2 cut(s) 541, 1180
BseMII CTCAG 4 cut(s) 270, 1260, 2187, 2472
BseNI ACTGG 5 cut(s) 882, 1312, 1801, 2448, 2498
BseRI GAGGAG 2 cut(s) 2330, 2333
BseSI GKGCMC 2 cut(s) 955, 2758
BseXI GCAGC 5 cut(s) 226, 1468, 1594, 2236, 2597
BseYI CCCAGC 3 cut(s) 242, 537, 2076
BsgI GTGCAG 1 cut(s) 1539
Bsh1236I CGCG 1 cut(s) 2400
BshFI GGCC 4 cut(s) 420, 564, 678, 2213
BshNI GGYRCC 2 cut(s) 952, 2010
BshTI ACCGGT 1 cut(s) 730
BshVI ATCGAT 1 cut(s) 2265
BsiHKAI GWGCWC 1 cut(s) 2758
BsiSI CCGG 8 cut(s) 57, 87, 186, 731, 822, 922, 1335, 2582
BsiWI CGTACG 1 cut(s) 136
BslFI GGGAC 3 cut(s) 301, 2004, 2048
BslI CCNNNNNNNGG 7 cut(s) 118, 877, 1307, 2077, 2200, 2201, 2220
BsmAI GTCTC 6 cut(s) 290, 622, 969, 1776, 2366, 2817
BsmBI CGTCTC 1 cut(s) 290
BsmFI GGGAC 3 cut(s) 301, 2004, 2048
BsmI GAATGC 1 cut(s) 2761
BsnI GGCC 4 cut(s) 420, 564, 678, 2213
Bso31I GGTCTC 1 cut(s) 1776
Bsp1286I GDGCHC 3 cut(s) 955, 2376, 2758
Bsp13I TCCGGA 1 cut(s) 821
BspANI GGCC 4 cut(s) 420, 564, 678, 2213
BspCNI CTCAG 4 cut(s) 271, 1259, 2188, 2471
BspDI ATCGAT 1 cut(s) 2265
BspEI TCCGGA 1 cut(s) 821
BspFNI CGCG 1 cut(s) 2400
BspHI TCATGA 4 cut(s) 1525, 1867, 2056, 2364
BspLI GGNNCC 5 cut(s) 289, 355, 954, 1312, 2012
BspMAI CTGCAG 1 cut(s) 631
BspMI ACCTGC 3 cut(s) 949, 2163, 2734
BspPI GGATC 8 cut(s) 348, 361, 465, 534, 590, 1807, 2059, 2088
BspQI GCTCTTC 1 cut(s) 2085
BspT107I GGYRCC 2 cut(s) 952, 2010
BspTI CTTAAG 2 cut(s) 1715, 2684
BspTNI GGTCTC 1 cut(s) 1776
BsrBI CCGCTC 1 cut(s) 417
BsrDI GCAATG 2 cut(s) 541, 1180
BsrFI RCCGGY 2 cut(s) 730, 2581
BsrI ACTGG 5 cut(s) 882, 1312, 1801, 2448, 2498
BssAI RCCGGY 2 cut(s) 730, 2581
BssECI CCNNGG 5 cut(s) 433, 776, 945, 2014, 2029
BssNI GRCGYC 1 cut(s) 45
BssT1I CCWWGG 1 cut(s) 945
Bst4CI ACNGT 9 cut(s) 339, 706, 805, 859, 1024, 1690, 1877, 2030, 2454
Bst6I CTCTTC 2 cut(s) 2085, 2813
BstACI GRCGYC 1 cut(s) 45
BstAFI CTTAAG 2 cut(s) 1715, 2684
BstAPI GCANNNNNTGC 1 cut(s) 547
BstBAI YACGTR 1 cut(s) 136
BstC8I GCNNGC 8 cut(s) 30, 92, 363, 548, 834, 914, 1063, 1319
BstDEI CTNAG 7 cut(s) 279, 641, 1241, 1246, 2196, 2215, 2458
BstDSI CCRYGG 3 cut(s) 433, 776, 2029
BstENI CCTNNNNNAGG 1 cut(s) 116
BstF5I GGATG 4 cut(s) 406, 886, 892, 1960
BstFNI CGCG 1 cut(s) 2400
BstH2I RGCGCY 1 cut(s) 2773
BstHHI GCGC 2 cut(s) 2402, 2772
BstMAI GTCTC 6 cut(s) 290, 622, 969, 1776, 2366, 2817
BstNSI RCATGY 3 cut(s) 550, 1348, 1918
BstSFI CTRYAG 3 cut(s) 585, 627, 1197
BstSLI GKGCMC 2 cut(s) 955, 2758
BstUI CGCG 1 cut(s) 2400
BstV1I GCAGC 5 cut(s) 226, 1468, 1594, 2236, 2597
BstV2I GAAGAC 2 cut(s) 31, 457
BstX2I RGATCY 6 cut(s) 353, 457, 526, 724, 1799, 2051
BstYI RGATCY 6 cut(s) 353, 457, 526, 724, 1799, 2051
Bsu15I ATCGAT 1 cut(s) 2265
Bsu36I CCTNAGG 1 cut(s) 2196
BsuI GTATCC 1 cut(s) 1759
BsuRI GGCC 4 cut(s) 420, 564, 678, 2213
BsuTUI ATCGAT 1 cut(s) 2265
BtgI CCRYGG 3 cut(s) 433, 776, 2029
BtgZI GCGATG 1 cut(s) 196
BtsCI GGATG 4 cut(s) 406, 886, 892, 1960
BtsI GCAGTG 2 cut(s) 327, 1452
BtsIMutI CAGTG 5 cut(s) 327, 1452, 1695, 1882, 2085
BveI ACCTGC 3 cut(s) 949, 2163, 2734
Cac8I GCNNGC 8 cut(s) 30, 92, 363, 548, 834, 914, 1063, 1319
CaiI CAGNNNCTG 1 cut(s) 2252
CciI TCATGA 4 cut(s) 1525, 1867, 2056, 2364
CfoI GCGC 2 cut(s) 2402, 2772
Cfr10I RCCGGY 2 cut(s) 730, 2581
Cfr13I GGNCC 2 cut(s) 288, 1933
ClaI ATCGAT 1 cut(s) 2265
CseI GACGC 3 cut(s) 34, 2294, 2446
Csp6I GTAC 6 cut(s) 13, 137, 153, 875, 1305, 2577
CspAI ACCGGT 1 cut(s) 730
CviQI GTAC 6 cut(s) 13, 137, 153, 875, 1305, 2577
DdeI CTNAG 7 cut(s) 279, 641, 1241, 1246, 2196, 2215, 2458
DraI TTTAAA 1 cut(s) 2350
DraIII CACNNNGTG 1 cut(s) 1460
DrdI GACNNNNNNGTC 1 cut(s) 2042
DseDI GACNNNNNNGTC 1 cut(s) 2042
EaeI YGGCCR 2 cut(s) 562, 676
Eam1104I CTCTTC 2 cut(s) 2085, 2813
EarI CTCTTC 2 cut(s) 2085, 2813
EciI GGCGGA 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 945
Eco24I GRGCYC 1 cut(s) 2376
Eco31I GGTCTC 1 cut(s) 1776
Eco47I GGWCC 2 cut(s) 288, 1933
Eco57I CTGAAG 3 cut(s) 2022, 2109, 2315
Eco81I CCTNAGG 1 cut(s) 2196
EcoNI CCTNNNNNAGG 1 cut(s) 116
EcoO109I RGGNCCY 1 cut(s) 288
EcoT14I CCWWGG 1 cut(s) 945
EcoT22I ATGCAT 1 cut(s) 2716
EcoT38I GRGCYC 1 cut(s) 2376
ErhI CCWWGG 1 cut(s) 945
Esp3I CGTCTC 1 cut(s) 290
FalI AAGNNNNNCTT 6 cut(s) 1417, 1449, 1685, 1717, 2604, 2636
FaqI GGGAC 3 cut(s) 301, 2004, 2048
FauI CCCGC 3 cut(s) 115, 706, 841
FbaI TGATCA 1 cut(s) 445
Fnu4HI GCNGC 7 cut(s) 33, 240, 418, 1482, 1583, 2250, 2586
FokI GGATG 4 cut(s) 413, 893, 899, 1967
FriOI GRGCYC 1 cut(s) 2376
Fsp4HI GCNGC 7 cut(s) 33, 240, 418, 1482, 1583, 2250, 2586
FspBI CTAG 4 cut(s) 1124, 1272, 1586, 2774
GlaI GCGC 2 cut(s) 2401, 2771
GluI GCNGC 7 cut(s) 33, 240, 418, 1482, 1583, 2250, 2586
GsaI CCCAGC 3 cut(s) 246, 541, 2080
HaeII RGCGCY 1 cut(s) 2773
HaeIII GGCC 4 cut(s) 420, 564, 678, 2213
HapII CCGG 8 cut(s) 57, 87, 186, 731, 822, 922, 1335, 2582
HgaI GACGC 3 cut(s) 34, 2294, 2446
HhaI GCGC 2 cut(s) 2402, 2772
Hin1I GRCGYC 1 cut(s) 45
Hin6I GCGC 2 cut(s) 2400, 2770
HinP1I GCGC 2 cut(s) 2400, 2770
HincII GTYRAC 2 cut(s) 1269, 2601
HindII GTYRAC 2 cut(s) 1269, 2601
HindIII AAGCTT 5 cut(s) 347, 359, 1040, 1718, 2471
HpaI GTTAAC 1 cut(s) 2601
HpaII CCGG 8 cut(s) 57, 87, 186, 731, 822, 922, 1335, 2582
HphI GGTGA 7 cut(s) 16, 64, 157, 422, 779, 911, 1853
Hpy166II GTNNAC 4 cut(s) 1269, 2579, 2601, 2756
Hpy8I GTNNAC 4 cut(s) 1269, 2579, 2601, 2756
Hpy99I CGWCG 2 cut(s) 1625, 2076
HpyAV CCTTC 5 cut(s) 107, 180, 995, 1687, 2482
HpyCH4III ACNGT 9 cut(s) 339, 706, 805, 859, 1024, 1690, 1877, 2030, 2454
HpyCH4IV ACGT 1 cut(s) 135
HpyF3I CTNAG 7 cut(s) 279, 641, 1241, 1246, 2196, 2215, 2458
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 1 cut(s) 45
HspAI GCGC 2 cut(s) 2400, 2770
Kpn2I TCCGGA 1 cut(s) 821
Ksp22I TGATCA 1 cut(s) 445
KspAI GTTAAC 1 cut(s) 2601
LguI GCTCTTC 1 cut(s) 2085
LmnI GCTCC 4 cut(s) 89, 109, 637, 2204
Lsp1109I GCAGC 5 cut(s) 226, 1468, 1594, 2236, 2597
MaeI CTAG 4 cut(s) 1124, 1272, 1586, 2774
MaeII ACGT 1 cut(s) 135
MbiI CCGCTC 1 cut(s) 417
MfeI CAATTG 1 cut(s) 1476
MflI RGATCY 6 cut(s) 353, 457, 526, 724, 1799, 2051
MhlI GDGCHC 3 cut(s) 955, 2376, 2758
MlsI TGGCCA 2 cut(s) 564, 678
MluNI TGGCCA 2 cut(s) 564, 678
MlyI GAGTC 4 cut(s) 626, 659, 804, 2153
MmeI TCCRAC 6 cut(s) 945, 973, 1131, 1366, 2112, 2655
Mox20I TGGCCA 2 cut(s) 564, 678
Mph1103I ATGCAT 1 cut(s) 2716
MroI TCCGGA 1 cut(s) 821
MroXI GAANNNNTTC 2 cut(s) 1697, 2391
MscI TGGCCA 2 cut(s) 564, 678
MslI CAYNNNNRTG 1 cut(s) 570
Msp20I TGGCCA 2 cut(s) 564, 678
MspA1I CMGCKG 2 cut(s) 1886, 2249
MspCI CTTAAG 2 cut(s) 1715, 2684
MspI CCGG 8 cut(s) 57, 87, 186, 731, 822, 922, 1335, 2582
MunI CAATTG 1 cut(s) 1476
Mva1269I GAATGC 1 cut(s) 2761
MvnI CGCG 1 cut(s) 2400
NlaIV GGNNCC 5 cut(s) 289, 355, 954, 1312, 2012
NmuCI GTSAC 6 cut(s) 202, 325, 1274, 1507, 1792, 2044
NsiI ATGCAT 1 cut(s) 2716
NspI RCATGY 3 cut(s) 550, 1348, 1918
OliI CACNNNNGTG 1 cut(s) 570
PaeI GCATGC 1 cut(s) 550
PagI TCATGA 4 cut(s) 1525, 1867, 2056, 2364
PaqCI CACCTGC 1 cut(s) 949
PciI ACATGT 1 cut(s) 1914
PciSI GCTCTTC 1 cut(s) 2085
PctI GAATGC 1 cut(s) 2761
PdmI GAANNNNTTC 2 cut(s) 1697, 2391
Pfl23II CGTACG 1 cut(s) 136
PflMI CCANNNNNTGG 2 cut(s) 877, 1307
PinAI ACCGGT 1 cut(s) 730
PkrI GCNGC 7 cut(s) 34, 241, 419, 1483, 1584, 2251, 2587
PleI GAGTC 4 cut(s) 626, 659, 803, 2152
PpsI GAGTC 4 cut(s) 626, 659, 803, 2152
Ppu21I YACGTR 1 cut(s) 136
PpuMI RGGWCCY 1 cut(s) 288
PscI ACATGT 1 cut(s) 1914
Psp5II RGGWCCY 1 cut(s) 288
PspFI CCCAGC 3 cut(s) 242, 537, 2076
PspLI CGTACG 1 cut(s) 136
PspN4I GGNNCC 5 cut(s) 289, 355, 954, 1312, 2012
PspPI GGNCC 2 cut(s) 288, 1933
PspPPI RGGWCCY 1 cut(s) 288
PsrI GAACNNNNNNTAC 2 cut(s) 1872, 1904
PstI CTGCAG 1 cut(s) 631
PstNI CAGNNNCTG 1 cut(s) 2252
PsuI RGATCY 6 cut(s) 353, 457, 526, 724, 1799, 2051
PvuII CAGCTG 2 cut(s) 1886, 2249
RsaI GTAC 6 cut(s) 14, 138, 154, 876, 1306, 2578
RsaNI GTAC 6 cut(s) 13, 137, 153, 875, 1305, 2577
RseI CAYNNNNRTG 1 cut(s) 570
SapI GCTCTTC 1 cut(s) 2085
SatI GCNGC 7 cut(s) 33, 240, 418, 1482, 1583, 2250, 2586
Sau96I GGNCC 2 cut(s) 288, 1933
ScaI AGTACT 1 cut(s) 1306
SchI GAGTC 4 cut(s) 626, 659, 804, 2153
SduI GDGCHC 3 cut(s) 955, 2376, 2758
SfcI CTRYAG 3 cut(s) 585, 627, 1197
SinI GGWCC 2 cut(s) 288, 1933
SmiMI CAYNNNNRTG 1 cut(s) 570
SmlI CTYRAG 5 cut(s) 1569, 1715, 1985, 2429, 2684
SmoI CTYRAG 5 cut(s) 1569, 1715, 1985, 2429, 2684
SpeI ACTAGT 1 cut(s) 1271
SphI GCATGC 1 cut(s) 550
SspI AATATT 1 cut(s) 1949
SspMI CTAG 4 cut(s) 1124, 1272, 1586, 2774
StyI CCWWGG 1 cut(s) 945
TaaI ACNGT 9 cut(s) 339, 706, 805, 859, 1024, 1690, 1877, 2030, 2454
TaiI ACGT 1 cut(s) 138
TaqI TCGA 8 cut(s) 114, 1057, 1112, 1167, 1891, 2265, 2702, 2809
TatI WGTACW 3 cut(s) 874, 1304, 2576
TauI GCSGC 2 cut(s) 35, 420
TscAI CASTG 5 cut(s) 334, 1452, 1695, 1882, 2092
TseFI GTSAC 6 cut(s) 202, 325, 1274, 1507, 1792, 2044
TseI GCWGC 5 cut(s) 239, 1481, 1582, 2249, 2585
Tsp45I GTSAC 6 cut(s) 202, 325, 1274, 1507, 1792, 2044
TspDTI ATGAA 8 cut(s) 725, 903, 1509, 1563, 1625, 1631, 2029, 2381
TspGWI ACGGA 7 cut(s) 8, 422, 470, 793, 1794, 2060, 2656
TspRI CASTG 5 cut(s) 334, 1452, 1695, 1882, 2092
Van91I CCANNNNNTGG 2 cut(s) 877, 1307
Vha464I CTTAAG 2 cut(s) 1715, 2684
VneI GTGCAC 1 cut(s) 2754
VpaK11BI GGWCC 2 cut(s) 288, 1933
XagI CCTNNNNNAGG 1 cut(s) 116
XapI RAATTY 6 cut(s) 267, 509, 826, 1154, 1813, 1964
XceI RCATGY 3 cut(s) 550, 1348, 1918
XcmI CCANNNNNNNNNTGG 2 cut(s) 1585, 1663
XmnI GAANNNNTTC 2 cut(s) 1697, 2391
XspI CTAG 4 cut(s) 1124, 1272, 1586, 2774
ZrmI AGTACT 1 cut(s) 1306
Zsp2I ATGCAT 1 cut(s) 2716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.