Rorug03G0174000

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0010840
Physical Location & Seq
Reverse (-)
14706196 .. 14708203
2008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0174000.1

Sequence Viewer

Length: 807 bp
ATGCGCGCCATGAAAATGCCTTCGAGAGCACTGTTTGGGTCATTCCTTGTCTTGTTTCTTGTTGGACATGCTGTCCATGGCCATGATGGTCAAAAGTTCCAGGCAGGTGAATGGAAACATGCTCATGCCACACACTATGAAGGAAGCTCCGACACATTTGGTGGAGCTTGTGGATACCATGATGTCGTTAAAGAAGGCTATGGCCTGGAGACAGTAGCATTGAGCAATGCATTGTTCAACAAGGGGGAGATGTGTGGAGCATGCTTCGAAATCAAATGCGAGGACTCGCCTGAATGGTGCAAGCCTGGCCAGCCAAGTCTCATTGTTACAGCAACCAACCAGTGCCCTCCGAATTACGACCAGCCGAGTGATAATGGAGGATGGTGCAACGAACCCCGTGAACATTTCGACATAGCAAGGCCAGTGTTCGAGACGATTGCCGCGTTCAAGGCCGGCATAGTTCCAGTCTCATACCGCAGGATTCCATGTGACCATAAGGAAGGAGGAATTAAATTTACAATAACTGGGAATCCTTATTTCAATGAAGTGTTGGTGTGGAATGTAGGAGGTGCTGGGGATATTTGCAACGTCGAAGTGAAAACTGAAGAGCAGCCGGATAAGTGGATACCGATGGAGCGAATGTGGGGTCAACGGTGGGTTTACAACGGAAAATTGTGTGAGAGTCAAGGCGCGCTTACCTTCCGAGTGACAGGGGGTGATGGAAGAAGCGTAACCTGTGACAATGTTGCCCCAAAGGGTTGGCAGTTTGGCCAAACTTATCAAGCCAAGAATAATTTCGAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000280 GO:0000923 GO:0000930 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0008092 GO:0008150 GO:0008275 GO:0009605 GO:0009607 GO:0009624 GO:0009898 GO:0009987 GO:0010638 GO:0010968 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0022402 GO:0022414 GO:0022607 GO:0030863 GO:0030981 GO:0031109 GO:0031110 GO:0031112 GO:0031113 GO:0031116 GO:0031122 GO:0031334 GO:0031967 GO:0031975 GO:0032153 GO:0032155 GO:0032271 GO:0032273 GO:0032886 GO:0032991 GO:0033043 GO:0034622 GO:0043015 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044450 GO:0044459 GO:0044464 GO:0046785 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051225 GO:0051258 GO:0051321 GO:0051415 GO:0051418 GO:0051493 GO:0051495 GO:0051704 GO:0051707 GO:0055028 GO:0065003 GO:0065007 GO:0070507 GO:0070925 GO:0071840 GO:0071944 GO:0090063 GO:0090307 GO:0097435 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1902850 GO:1902903 GO:1902905 GO:1903047
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spc97_Spc98

Protein Analysis

268

Amino Acids

29.74

Weight (kDa)

5.99

Isoelectric Point (pI)

33.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 42 - 165 2.4e-10 EXPB1-like domain 1
DPBB_1 PF03330 71 - 158 1.8e-18 Lytic transglycolase
Expansin_C PF01357 170 - 251 2e-24 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 95
Acc36I ACCTGC 1 cut(s) 95
AccII CGCG 3 cut(s) 6, 443, 692
AciI CCGC 2 cut(s) 441, 475
AcoI YGGCCR 3 cut(s) 79, 307, 769
AcsI RAATTY 1 cut(s) 512
AcuI CTGAAG 1 cut(s) 624
AgsI TTSAA 3 cut(s) 238, 448, 541
AhdI GACNNNNNGTC 1 cut(s) 71
AjnI CCWGG 3 cut(s) 99, 204, 304
AjuI GAANNNNNNNTTGG 1 cut(s) 779
AluBI AGCT 2 cut(s) 147, 167
AluI AGCT 2 cut(s) 147, 167
Alw21I GWGCWC 1 cut(s) 31
Alw26I GTCTC 4 cut(s) 203, 323, 425, 472
AoxI GGCC 6 cut(s) 79, 202, 307, 419, 450, 769
ApeKI GCWGC 1 cut(s) 610
ApoI RAATTY 1 cut(s) 512
ArsI GACNNNNNNTTYG 2 cut(s) 631, 663
Asp700I GAANNNNTTC 1 cut(s) 794
AspLEI GCGC 4 cut(s) 6, 8, 692, 694
AsuHPI GGTGA 2 cut(s) 119, 728
AsuII TTCGAA 2 cut(s) 267, 798
BaeGI GKGCMC 1 cut(s) 347
BalI TGGCCA 3 cut(s) 81, 309, 771
Bbv12I GWGCWC 1 cut(s) 31
BbvI GCAGC 1 cut(s) 622
BccI CCATC 4 cut(s) 80, 375, 625, 713
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 3 cut(s) 101, 206, 306
BciVI GTATCC 2 cut(s) 167, 618
BcoDI GTCTC 4 cut(s) 203, 323, 425, 472
BfuAI ACCTGC 1 cut(s) 95
BfuI GTATCC 2 cut(s) 167, 618
BisI GCNGC 2 cut(s) 441, 611
BlsI GCNGC 2 cut(s) 442, 612
Bme1390I CCNGG 3 cut(s) 101, 206, 306
BmeRI GACNNNNNGTC 1 cut(s) 71
BmrFI CCNGG 3 cut(s) 101, 206, 306
BmrI ACTGGG 1 cut(s) 534
BmuI ACTGGG 1 cut(s) 534
BpmI CTGGAG 1 cut(s) 227
Bpu14I TTCGAA 2 cut(s) 267, 798
BsaJI CCNNGG 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bse118I RCCGGY 1 cut(s) 452
Bse1I ACTGG 4 cut(s) 340, 422, 464, 529
Bse3DI GCAATG 1 cut(s) 232
BseBI CCWGG 3 cut(s) 101, 206, 306
BseDI CCNNGG 1 cut(s) 76
BseGI GGATG 1 cut(s) 386
BseMI GCAATG 1 cut(s) 232
BseNI ACTGG 4 cut(s) 340, 422, 464, 529
BsePI GCGCGC 2 cut(s) 4, 690
BseSI GKGCMC 1 cut(s) 347
BseXI GCAGC 1 cut(s) 622
BseYI CCCAGC 1 cut(s) 572
Bsh1236I CGCG 3 cut(s) 6, 443, 692
BshFI GGCC 6 cut(s) 81, 204, 309, 421, 452, 771
BsiHKAI GWGCWC 1 cut(s) 31
BsiSI CCGG 2 cut(s) 453, 614
BsmAI GTCTC 4 cut(s) 203, 323, 425, 472
BsmBI CGTCTC 1 cut(s) 425
BsnI GGCC 6 cut(s) 81, 204, 309, 421, 452, 771
Bsp119I TTCGAA 2 cut(s) 267, 798
Bsp1286I GDGCHC 2 cut(s) 31, 347
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 2 cut(s) 441, 475
BspANI GGCC 6 cut(s) 81, 204, 309, 421, 452, 771
BspFNI CGCG 3 cut(s) 6, 443, 692
BspMI ACCTGC 1 cut(s) 95
BspQI GCTCTTC 1 cut(s) 600
BspT104I TTCGAA 2 cut(s) 267, 798
BsrDI GCAATG 1 cut(s) 232
BsrFI RCCGGY 1 cut(s) 452
BsrI ACTGG 4 cut(s) 340, 422, 464, 529
BssAI RCCGGY 1 cut(s) 452
BssECI CCNNGG 1 cut(s) 76
BssHII GCGCGC 2 cut(s) 4, 690
BssT1I CCWWGG 1 cut(s) 76
Bst2UI CCWGG 3 cut(s) 101, 206, 306
Bst4CI ACNGT 3 cut(s) 33, 214, 654
Bst6I CTCTTC 1 cut(s) 600
BstBI TTCGAA 2 cut(s) 267, 798
BstC8I GCNNGC 6 cut(s) 6, 262, 302, 311, 454, 692
BstDSI CCRYGG 1 cut(s) 76
BstF5I GGATG 1 cut(s) 386
BstFNI CGCG 3 cut(s) 6, 443, 692
BstHHI GCGC 4 cut(s) 6, 8, 692, 694
BstMAI GTCTC 4 cut(s) 203, 323, 425, 472
BstMWI GCNNNNNNNGC 3 cut(s) 306, 310, 449
BstNI CCWGG 3 cut(s) 101, 206, 306
BstNSI RCATGY 3 cut(s) 71, 122, 264
BstSCI CCNGG 3 cut(s) 99, 204, 304
BstSLI GKGCMC 1 cut(s) 347
BstUI CGCG 3 cut(s) 6, 443, 692
BstV1I GCAGC 1 cut(s) 622
BstXI CCANNNNNNTGG 1 cut(s) 759
BsuI GTATCC 2 cut(s) 167, 618
BsuRI GGCC 6 cut(s) 81, 204, 309, 421, 452, 771
BtgI CCRYGG 1 cut(s) 76
BtsCI GGATG 1 cut(s) 386
BtsIMutI CAGTG 3 cut(s) 29, 347, 429
BveI ACCTGC 1 cut(s) 95
Cac8I GCNNGC 6 cut(s) 6, 262, 302, 311, 454, 692
CfoI GCGC 4 cut(s) 6, 8, 692, 694
Cfr10I RCCGGY 1 cut(s) 452
CviAII CATG 9 cut(s) 10, 68, 77, 83, 119, 125, 179, 261, 486
DriI GACNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 3 cut(s) 79, 307, 769
Eam1104I CTCTTC 1 cut(s) 600
Eam1105I GACNNNNNGTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 600
Eco130I CCWWGG 1 cut(s) 76
Eco57I CTGAAG 1 cut(s) 624
EcoRII CCWGG 3 cut(s) 99, 204, 304
EcoT14I CCWWGG 1 cut(s) 76
EcoT22I ATGCAT 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 76
Esp3I CGTCTC 1 cut(s) 425
FaeI CATG 9 cut(s) 13, 71, 80, 86, 122, 128, 182, 264, 489
FalI AAGNNNNNCTT 2 cut(s) 678, 710
FatI CATG 9 cut(s) 9, 67, 76, 82, 118, 124, 178, 260, 485
Fnu4HI GCNGC 2 cut(s) 441, 611
FokI GGATG 1 cut(s) 393
Fsp4HI GCNGC 2 cut(s) 441, 611
GlaI GCGC 4 cut(s) 5, 7, 691, 693
GluI GCNGC 2 cut(s) 441, 611
GsaI CCCAGC 1 cut(s) 576
GsuI CTGGAG 1 cut(s) 227
HaeIII GGCC 6 cut(s) 81, 204, 309, 421, 452, 771
HapII CCGG 2 cut(s) 453, 614
HhaI GCGC 4 cut(s) 6, 8, 692, 694
Hin1II CATG 9 cut(s) 13, 71, 80, 86, 122, 128, 182, 264, 489
Hin6I GCGC 4 cut(s) 4, 6, 690, 692
HinP1I GCGC 4 cut(s) 4, 6, 690, 692
HincII GTYRAC 1 cut(s) 650
HindII GTYRAC 1 cut(s) 650
HinfI GANTC 4 cut(s) 284, 481, 529, 682
HpaII CCGG 2 cut(s) 453, 614
HphI GGTGA 2 cut(s) 119, 728
Hpy166II GTNNAC 3 cut(s) 401, 650, 661
Hpy188I TCNGA 3 cut(s) 151, 351, 704
Hpy188III TCNNGA 2 cut(s) 24, 430
Hpy8I GTNNAC 3 cut(s) 401, 650, 661
Hpy99I CGWCG 1 cut(s) 593
HpyAV CCTTC 5 cut(s) 30, 134, 188, 494, 709
HpyCH4III ACNGT 3 cut(s) 33, 214, 654
HpyCH4IV ACGT 1 cut(s) 588
HpyCH4V TGCA 4 cut(s) 230, 300, 387, 585
HpyF10VI GCNNNNNNNGC 3 cut(s) 306, 310, 449
HpySE526I ACGT 1 cut(s) 588
Hsp92II CATG 9 cut(s) 13, 71, 80, 86, 122, 128, 182, 264, 489
HspAI GCGC 4 cut(s) 4, 6, 690, 692
KroI GCCGGC 1 cut(s) 452
KroNI GCCGGC 1 cut(s) 454
LguI GCTCTTC 1 cut(s) 600
LmnI GCTCC 4 cut(s) 152, 164, 257, 634
Lsp1109I GCAGC 1 cut(s) 622
MaeII ACGT 1 cut(s) 588
MaeIII GTNAC 5 cut(s) 325, 488, 706, 730, 737
MboII GAAGA 2 cut(s) 617, 735
MhlI GDGCHC 2 cut(s) 31, 347
MlsI TGGCCA 3 cut(s) 81, 309, 771
MluCI AATT 5 cut(s) 352, 507, 512, 671, 793
MluNI TGGCCA 3 cut(s) 81, 309, 771
MlyI GAGTC 2 cut(s) 278, 691
MmeI TCCRAC 2 cut(s) 43, 174
MnlI CCTC 5 cut(s) 274, 357, 371, 497, 560
Mox20I TGGCCA 3 cut(s) 81, 309, 771
Mph1103I ATGCAT 1 cut(s) 232
MroNI GCCGGC 1 cut(s) 452
MroXI GAANNNNTTC 1 cut(s) 794
MscI TGGCCA 3 cut(s) 81, 309, 771
MseI TTAA 2 cut(s) 189, 510
MslI CAYNNNNRTG 3 cut(s) 14, 81, 123
Msp20I TGGCCA 3 cut(s) 81, 309, 771
MspI CCGG 2 cut(s) 453, 614
MspR9I CCNGG 3 cut(s) 101, 206, 306
MvaI CCWGG 3 cut(s) 101, 206, 306
MvnI CGCG 3 cut(s) 6, 443, 692
MwoI GCNNNNNNNGC 3 cut(s) 306, 310, 449
NaeI GCCGGC 1 cut(s) 454
NcoI CCATGG 1 cut(s) 76
NgoMIV GCCGGC 1 cut(s) 452
NlaIII CATG 9 cut(s) 13, 71, 80, 86, 122, 128, 182, 264, 489
NmeAIII GCCGAG 1 cut(s) 390
NmuCI GTSAC 3 cut(s) 488, 706, 737
NsiI ATGCAT 1 cut(s) 232
NspI RCATGY 3 cut(s) 71, 122, 264
NspV TTCGAA 2 cut(s) 267, 798
PaeI GCATGC 1 cut(s) 264
PaqCI CACCTGC 1 cut(s) 95
PauI GCGCGC 2 cut(s) 4, 690
PciSI GCTCTTC 1 cut(s) 600
PcsI WCGNNNNNNNCGW 1 cut(s) 440
PdiI GCCGGC 1 cut(s) 454
PdmI GAANNNNTTC 1 cut(s) 794
PfeI GAWTC 2 cut(s) 481, 529
PkrI GCNGC 2 cut(s) 442, 612
PleI GAGTC 2 cut(s) 278, 690
PpsI GAGTC 2 cut(s) 278, 690
Psp6I CCWGG 3 cut(s) 99, 204, 304
PspFI CCCAGC 1 cut(s) 572
PspGI CCWGG 3 cut(s) 99, 204, 304
PteI GCGCGC 2 cut(s) 4, 690
RseI CAYNNNNRTG 3 cut(s) 14, 81, 123
SapI GCTCTTC 1 cut(s) 600
SaqAI TTAA 2 cut(s) 189, 510
SatI GCNGC 2 cut(s) 441, 611
SchI GAGTC 2 cut(s) 278, 691
ScrFI CCNGG 3 cut(s) 101, 206, 306
SduI GDGCHC 2 cut(s) 31, 347
SetI ASST 7 cut(s) 109, 149, 169, 571, 591, 701, 737
SfuI TTCGAA 2 cut(s) 267, 798
SmiMI CAYNNNNRTG 3 cut(s) 14, 81, 123
SphI GCATGC 1 cut(s) 264
Sse9I AATT 5 cut(s) 352, 507, 512, 671, 793
SsiI CCGC 2 cut(s) 441, 475
StyD4I CCNGG 3 cut(s) 99, 204, 304
StyI CCWWGG 1 cut(s) 76
TaaI ACNGT 3 cut(s) 33, 214, 654
TaiI ACGT 1 cut(s) 591
TaqI TCGA 6 cut(s) 23, 267, 408, 429, 591, 798
TasI AATT 5 cut(s) 352, 507, 512, 671, 793
TauI GCSGC 1 cut(s) 443
TfiI GAWTC 2 cut(s) 481, 529
Tru1I TTAA 2 cut(s) 189, 510
Tru9I TTAA 2 cut(s) 189, 510
TscAI CASTG 3 cut(s) 36, 347, 429
TseFI GTSAC 3 cut(s) 488, 706, 737
TseI GCWGC 1 cut(s) 610
Tsp45I GTSAC 3 cut(s) 488, 706, 737
TspDTI ATGAA 3 cut(s) 26, 153, 558
TspGWI ACGGA 1 cut(s) 681
TspRI CASTG 3 cut(s) 36, 347, 429
XapI RAATTY 1 cut(s) 512
XceI RCATGY 3 cut(s) 71, 122, 264
XcmI CCANNNNNNNNNTGG 1 cut(s) 83
XmnI GAANNNNTTC 1 cut(s) 794
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.