Rorug03G0173900

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0010840
Physical Location & Seq
Reverse (-)
14699511 .. 14700617
1107 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0173900.1

Sequence Viewer

Length: 783 bp
ATGGCGTCAATGTGTTCTGCCCTCCTGGGTTCATTGCTTTTCATTCTGCTCGTGTTACCTGACCATGTCACCGCTCGCCATAAGCCAAAGTTCAGACCTGGCCCTTGGAAGCATGCTCATGCCACATTCTACGAAGGCGGCTCTGGATCATTTGGTGGTGCTTGTGGTTACCACGACGTCGTTCAAGAAGGCTATGGCCTCGACACGGTGGCTTTGAGCGACACCTTGTTCAACAATGGGCAGTCGTGTGGTGCATGTTACGATATCCAATGTGTGGACTCCCCACAATGGTGCAAGCCCGGGCAGCCAACTCTTCATGTTACAGCAACCAACCATTGCCCCCCGAATTATAACCTTCCCAGTGACAATGGAGGTTGGTGCAATGCACCACGTGAGCACTTCGATATAGCCAAGCCGGTGTTCCTCAACATTGCCGAGTACAAGGCTGGCATAGTACCAATCATATACCGCAGGGTTCCGTGCCAAAAGAGAGGAGGCATCCGATTCACGATAACCGGGAATGCTTACTTCAATGAAGTGTTGGTGTGGAACGTGGGAGGTGCCGGAGATGTGATAAGCGTGCAAGTGAAGGGCCATGACAAGCTCAAATGGACAACGATGAAGCGAATGTGGGGTCAGAGGTGGATTACTGATGCCAAGTTGGTTGGGGAGTCTCTGACCTTCAGAGTTGAAGCAAGTGATAAAAGACGCTCAACTTCATGGCATATAGCCCCTCAGAACTGGCAGTTTGGCCAGACCTTTGAAGGCAAGAACTTCCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000280 GO:0000923 GO:0000930 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0008092 GO:0008150 GO:0008275 GO:0009605 GO:0009607 GO:0009624 GO:0009898 GO:0009987 GO:0010638 GO:0010968 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0022402 GO:0022414 GO:0022607 GO:0030863 GO:0030981 GO:0031109 GO:0031110 GO:0031112 GO:0031113 GO:0031116 GO:0031122 GO:0031334 GO:0031967 GO:0031975 GO:0032153 GO:0032155 GO:0032271 GO:0032273 GO:0032886 GO:0032991 GO:0033043 GO:0034622 GO:0043015 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044450 GO:0044459 GO:0044464 GO:0046785 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051225 GO:0051258 GO:0051321 GO:0051415 GO:0051418 GO:0051493 GO:0051495 GO:0051704 GO:0051707 GO:0055028 GO:0065003 GO:0065007 GO:0070507 GO:0070925 GO:0071840 GO:0071944 GO:0090063 GO:0090307 GO:0097435 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1902850 GO:1902903 GO:1902905 GO:1903047
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spc97_Spc98

Protein Analysis

260

Amino Acids

28.95

Weight (kDa)

8.95

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 41 - 162 4.2e-06 EXPB1-like domain 1
DPBB_1 PF03330 69 - 156 9.6e-18 Lytic transglycolase
Expansin_C PF01357 167 - 245 1e-23 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 351
AatII GACGTC 1 cut(s) 180
AccB1I GGYRCC 1 cut(s) 560
AccB7I CCANNNNNTGG 1 cut(s) 274
AccBSI CCGCTC 1 cut(s) 74
AciI CCGC 3 cut(s) 72, 138, 469
AclWI GGATC 1 cut(s) 154
AcoI YGGCCR 1 cut(s) 751
AcuI CTGAAG 1 cut(s) 667
AcvI CACGTG 1 cut(s) 392
AcyI GRCGYC 2 cut(s) 5, 177
AdeI CACNNNGTG 1 cut(s) 392
AfaI GTAC 2 cut(s) 440, 456
AfiI CCNNNNNNNGG 3 cut(s) 205, 274, 288
AgsI TTSAA 5 cut(s) 185, 232, 532, 692, 764
AjnI CCWGG 2 cut(s) 24, 97
AjuI GAANNNNNNNTTGG 2 cut(s) 404, 436
AleI CACNNNNGTG 1 cut(s) 289
AluBI AGCT 1 cut(s) 604
AluI AGCT 1 cut(s) 604
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 1 cut(s) 678
AlwI GGATC 1 cut(s) 154
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 4 cut(s) 100, 196, 592, 751
ApeKI GCWGC 1 cut(s) 304
ArsI GACNNNNNNTTYG 2 cut(s) 619, 651
AspS9I GGNCC 2 cut(s) 101, 592
AsuC2I CCSGG 3 cut(s) 300, 301, 517
AsuHPI GGTGA 1 cut(s) 61
AvaI CYCGRG 1 cut(s) 299
BalI TGGCCA 1 cut(s) 753
BanI GGYRCC 1 cut(s) 560
BauI CACGAG 1 cut(s) 50
BbrPI CACGTG 1 cut(s) 392
Bbv12I GWGCWC 1 cut(s) 399
BbvI GCAGC 1 cut(s) 316
BciT130I CCWGG 2 cut(s) 26, 99
BcnI CCSGG 3 cut(s) 300, 301, 517
BcoDI GTCTC 1 cut(s) 678
BisI GCNGC 2 cut(s) 139, 305
BlsI GCNGC 2 cut(s) 140, 306
Bme1390I CCNGG 5 cut(s) 26, 99, 300, 301, 517
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 2 cut(s) 101, 592
BmiI GGNNCC 2 cut(s) 477, 562
BmrFI CCNGG 5 cut(s) 26, 99, 300, 301, 517
BmrI ACTGGG 1 cut(s) 354
BmsI GCATC 2 cut(s) 507, 643
BmuI ACTGGG 1 cut(s) 354
BpuMI CCSGG 3 cut(s) 300, 301, 517
BsaAI YACGTR 1 cut(s) 392
BsaHI GRCGYC 2 cut(s) 5, 177
BsaJI CCNNGG 3 cut(s) 25, 104, 299
Bsc4I CCNNNNNNNGG 3 cut(s) 205, 274, 288
Bse118I RCCGGY 1 cut(s) 415
Bse1I ACTGG 2 cut(s) 360, 746
Bse3DI GCAATG 4 cut(s) 32, 334, 388, 429
BseBI CCWGG 2 cut(s) 26, 99
BseDI CCNNGG 3 cut(s) 25, 104, 299
BseGI GGATG 1 cut(s) 498
BseLI CCNNNNNNNGG 3 cut(s) 205, 274, 288
BseMI GCAATG 4 cut(s) 32, 334, 388, 429
BseMII CTCAG 1 cut(s) 749
BseNI ACTGG 2 cut(s) 360, 746
BseRI GAGGAG 1 cut(s) 507
BseXI GCAGC 1 cut(s) 316
BshFI GGCC 4 cut(s) 102, 198, 594, 753
BshNI GGYRCC 1 cut(s) 560
BsiHKAI GWGCWC 1 cut(s) 399
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 4 cut(s) 300, 416, 516, 564
BslI CCNNNNNNNGG 3 cut(s) 205, 274, 288
BsmAI GTCTC 1 cut(s) 678
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 4 cut(s) 102, 198, 594, 753
BsoBI CYCGRG 1 cut(s) 299
Bsp1286I GDGCHC 1 cut(s) 399
Bsp143I GATC 1 cut(s) 146
BspACI CCGC 3 cut(s) 72, 138, 469
BspANI GGCC 4 cut(s) 102, 198, 594, 753
BspCNI CTCAG 1 cut(s) 748
BspLI GGNNCC 2 cut(s) 477, 562
BspPI GGATC 1 cut(s) 154
BspT107I GGYRCC 1 cut(s) 560
BsrBI CCGCTC 1 cut(s) 74
BsrDI GCAATG 4 cut(s) 32, 334, 388, 429
BsrFI RCCGGY 1 cut(s) 415
BsrI ACTGG 2 cut(s) 360, 746
BssAI RCCGGY 1 cut(s) 415
BssECI CCNNGG 3 cut(s) 25, 104, 299
BssMI GATC 1 cut(s) 146
BssNI GRCGYC 2 cut(s) 5, 177
BssSI CACGAG 1 cut(s) 50
BssT1I CCWWGG 1 cut(s) 104
Bst2BI CACGAG 1 cut(s) 50
Bst2UI CCWGG 2 cut(s) 26, 99
Bst4CI ACNGT 1 cut(s) 208
Bst6I CTCTTC 1 cut(s) 318
BstACI GRCGYC 2 cut(s) 5, 177
BstBAI YACGTR 1 cut(s) 392
BstC8I GCNNGC 5 cut(s) 76, 114, 296, 448, 581
BstDEI CTNAG 1 cut(s) 735
BstEII GGTNACC 1 cut(s) 167
BstF5I GGATG 1 cut(s) 498
BstKTI GATC 1 cut(s) 149
BstMAI GTCTC 1 cut(s) 678
BstMBI GATC 1 cut(s) 146
BstMWI GCNNNNNNNGC 1 cut(s) 304
BstNI CCWGG 2 cut(s) 26, 99
BstNSI RCATGY 2 cut(s) 116, 258
BstPI GGTNACC 1 cut(s) 167
BstSCI CCNGG 5 cut(s) 24, 97, 298, 299, 515
BstV1I GCAGC 1 cut(s) 316
BsuRI GGCC 4 cut(s) 102, 198, 594, 753
BtsCI GGATG 1 cut(s) 498
BtsIMutI CAGTG 1 cut(s) 367
Cac8I GCNNGC 5 cut(s) 76, 114, 296, 448, 581
Cfr10I RCCGGY 1 cut(s) 415
Cfr13I GGNCC 2 cut(s) 101, 592
Cfr9I CCCGGG 1 cut(s) 299
CseI GACGC 1 cut(s) 717
Csp6I GTAC 2 cut(s) 439, 455
CviAII CATG 7 cut(s) 65, 113, 119, 255, 317, 596, 720
CviQI GTAC 2 cut(s) 439, 455
DdeI CTNAG 1 cut(s) 735
DpnI GATC 1 cut(s) 148
DpnII GATC 1 cut(s) 146
DraIII CACNNNGTG 1 cut(s) 392
EaeI YGGCCR 1 cut(s) 751
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 104
Eco32I GATATC 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 667
Eco72I CACGTG 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 299
Eco91I GGTNACC 1 cut(s) 167
EcoO65I GGTNACC 1 cut(s) 167
EcoRII CCWGG 2 cut(s) 24, 97
EcoRV GATATC 1 cut(s) 265
EcoT14I CCWWGG 1 cut(s) 104
ErhI CCWWGG 1 cut(s) 104
FaeI CATG 7 cut(s) 68, 116, 122, 258, 320, 599, 723
FatI CATG 7 cut(s) 64, 112, 118, 254, 316, 595, 719
Fnu4HI GCNGC 2 cut(s) 139, 305
FokI GGATG 1 cut(s) 485
Fsp4HI GCNGC 2 cut(s) 139, 305
GluI GCNGC 2 cut(s) 139, 305
HaeIII GGCC 4 cut(s) 102, 198, 594, 753
HapII CCGG 4 cut(s) 300, 416, 516, 564
HgaI GACGC 1 cut(s) 717
Hin1I GRCGYC 2 cut(s) 5, 177
Hin1II CATG 7 cut(s) 68, 116, 122, 258, 320, 599, 723
HinfI GANTC 3 cut(s) 278, 504, 671
HpaII CCGG 4 cut(s) 300, 416, 516, 564
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 7 cut(s) 95, 503, 639, 678, 686, 738, 779
Hpy188III TCNNGA 3 cut(s) 144, 185, 508
Hpy8I GTNNAC 1 cut(s) 277
Hpy99I CGWCG 2 cut(s) 179, 182
HpyAV CCTTC 6 cut(s) 128, 182, 365, 583, 691, 758
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 3 cut(s) 177, 391, 552
HpyCH4V TGCA 5 cut(s) 254, 294, 381, 386, 583
HpyF10VI GCNNNNNNNGC 1 cut(s) 304
HpyF3I CTNAG 1 cut(s) 735
HpySE526I ACGT 3 cut(s) 177, 391, 552
Hsp92I GRCGYC 2 cut(s) 5, 177
Hsp92II CATG 7 cut(s) 68, 116, 122, 258, 320, 599, 723
Kzo9I GATC 1 cut(s) 146
Lsp1109I GCAGC 1 cut(s) 316
LweI GCATC 2 cut(s) 507, 643
MaeII ACGT 3 cut(s) 177, 391, 552
MaeIII GTNAC 6 cut(s) 54, 67, 167, 257, 319, 362
MalI GATC 1 cut(s) 148
MbiI CCGCTC 1 cut(s) 74
MboI GATC 1 cut(s) 146
MboII GAAGA 1 cut(s) 305
MhlI GDGCHC 1 cut(s) 399
MlsI TGGCCA 1 cut(s) 753
MluCI AATT 1 cut(s) 346
MluNI TGGCCA 1 cut(s) 753
MlyI GAGTC 2 cut(s) 272, 680
MnlI CCTC 9 cut(s) 32, 209, 365, 434, 485, 488, 551, 633, 744
Mox20I TGGCCA 1 cut(s) 753
MscI TGGCCA 1 cut(s) 753
MslI CAYNNNNRTG 2 cut(s) 117, 289
Msp20I TGGCCA 1 cut(s) 753
MspI CCGG 4 cut(s) 300, 416, 516, 564
MspR9I CCNGG 5 cut(s) 26, 99, 300, 301, 517
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 2 cut(s) 26, 99
MwoI GCNNNNNNNGC 1 cut(s) 304
NciI CCSGG 3 cut(s) 300, 301, 517
NdeII GATC 1 cut(s) 146
NlaIII CATG 7 cut(s) 68, 116, 122, 258, 320, 599, 723
NlaIV GGNNCC 2 cut(s) 477, 562
NmeAIII GCCGAG 1 cut(s) 460
NmuCI GTSAC 2 cut(s) 67, 362
NspI RCATGY 2 cut(s) 116, 258
OliI CACNNNNGTG 1 cut(s) 289
PaeI GCATGC 1 cut(s) 116
PctI GAATGC 1 cut(s) 526
PfeI GAWTC 1 cut(s) 504
PflFI GACNNNGTC 1 cut(s) 65
PflMI CCANNNNNTGG 1 cut(s) 274
PkrI GCNGC 2 cut(s) 140, 306
PleI GAGTC 2 cut(s) 272, 679
PmaCI CACGTG 1 cut(s) 392
PmlI CACGTG 1 cut(s) 392
PpsI GAGTC 2 cut(s) 272, 679
Ppu21I YACGTR 1 cut(s) 392
PsiI TTATAA 1 cut(s) 351
Psp6I CCWGG 2 cut(s) 24, 97
PspCI CACGTG 1 cut(s) 392
PspEI GGTNACC 1 cut(s) 167
PspGI CCWGG 2 cut(s) 24, 97
PspN4I GGNNCC 2 cut(s) 477, 562
PspPI GGNCC 2 cut(s) 101, 592
PsyI GACNNNGTC 1 cut(s) 65
RsaI GTAC 2 cut(s) 440, 456
RsaNI GTAC 2 cut(s) 439, 455
RseI CAYNNNNRTG 2 cut(s) 117, 289
SatI GCNGC 2 cut(s) 139, 305
Sau3AI GATC 1 cut(s) 146
Sau96I GGNCC 2 cut(s) 101, 592
SchI GAGTC 2 cut(s) 272, 680
ScrFI CCNGG 5 cut(s) 26, 99, 300, 301, 517
SduI GDGCHC 1 cut(s) 399
SfaNI GCATC 2 cut(s) 507, 643
SmaI CCCGGG 1 cut(s) 301
SmiMI CAYNNNNRTG 2 cut(s) 117, 289
SphI GCATGC 1 cut(s) 116
SrfI GCCCGGGC 1 cut(s) 301
Sse9I AATT 1 cut(s) 346
SsiI CCGC 3 cut(s) 72, 138, 469
StyD4I CCNGG 5 cut(s) 24, 97, 298, 299, 515
StyI CCWWGG 1 cut(s) 104
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 3 cut(s) 180, 394, 555
TaqI TCGA 2 cut(s) 201, 402
TasI AATT 1 cut(s) 346
TatI WGTACW 1 cut(s) 438
TauI GCSGC 1 cut(s) 141
TfiI GAWTC 1 cut(s) 504
TscAI CASTG 1 cut(s) 367
TseFI GTSAC 2 cut(s) 67, 362
TseI GCWGC 1 cut(s) 304
Tsp45I GTSAC 2 cut(s) 67, 362
TspDTI ATGAA 6 cut(s) 21, 31, 305, 549, 635, 708
TspGWI ACGGA 1 cut(s) 468
TspMI CCCGGG 1 cut(s) 299
TspRI CASTG 1 cut(s) 367
Tth111I GACNNNGTC 1 cut(s) 65
Van91I CCANNNNNTGG 1 cut(s) 274
XceI RCATGY 2 cut(s) 116, 258
XmaI CCCGGG 1 cut(s) 299
ZraI GACGTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.