Rroxscaffold_2G00107120

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010840
Physical Location & Seq
Forward (+)
30824652 .. 30829102
4451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00107120.1

Sequence Viewer

Length: 963 bp
ATGTTCAATACTTCAAGATTGACCGCCTCCGTCACCCCGGACGCCGCCGCCATCGCCGAGTCCACCAAATGCCGACTCGCCACTCAAGGTAAGTCCTCCCAAGCCCTAACTTTCGCCGACCTTTATACTCGGTTCGCATCCAAAACGGGCCCCGAAAGTGTAAATAACAAGTTGGCAGTTCTTTATTTGCTTAAGATTATATCGGAGGATCGAAAGAGTGTGAATTCCCGAGTTGGATTCTTCGGTTTTGTTGCCCAATTTGGGTTTAAACAACGTCGGATCAAGCGGCGAGTCGAGGGTTTTGGGGGGAAGGGGAAGTGGGGAAAAGGGCTGCAGCAATGGGGTTTTCCCAACAAGGCAGTCCAAAGAGAAGAGGCCCACCGAAGGGTCCAGCAAGACAAGGTGGACACCAAGCCCAAACAAGCAACTCCACCACGCTGCAGTCTCCCGTGCATTGCTGCCCACGACTCCGGCCATGAACCGGCACCTCCTCCCGCCCTCGCCATGACAAAGGTCTTTGTTAAGCTCCGCCATGAGCCGGCAAAGCCCCGAGGCCCAAGAGCCTTCGTCAATGCGGCGCCTAGCCCCGAGTCCCACGTGCCCGCACCAAGCCACGCTACCGCCGGATATGGAAACGCTCGTCCCCGCACGGAAAGCCACCACAAACGGCTAGAGGAGCCGCCCATATCTGCCGTCACCCGGCCGCCAACCCCCAGCCCAAAGTCCTCCGGACCTCATCCCGGGCGTCGAAGTACCGTCCCGGCAGCCGGCATAGTGGAGGTTTGTGGGAGCTTGGTTCCTTTTCTTTCGGTGTGGGACGCTCGGGATCCCGATATTGCCCCGAGGGGTATCGGAACCCCCAGTGGGAGCTTACTTCGCCGGCGTATTGGCGATACGAGTCTTGTGCTTCCGATACTTGTGCCTAAGAGGTATGTGCATACCAACGGTGACATTGTTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000280 GO:0000923 GO:0000930 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0008092 GO:0008150 GO:0008275 GO:0009605 GO:0009607 GO:0009624 GO:0009898 GO:0009987 GO:0010638 GO:0010968 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0022402 GO:0022414 GO:0022607 GO:0030863 GO:0030981 GO:0031109 GO:0031110 GO:0031112 GO:0031113 GO:0031116 GO:0031122 GO:0031334 GO:0031967 GO:0031975 GO:0032153 GO:0032155 GO:0032271 GO:0032273 GO:0032886 GO:0032991 GO:0033043 GO:0034622 GO:0043015 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044450 GO:0044459 GO:0044464 GO:0046785 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051225 GO:0051258 GO:0051321 GO:0051415 GO:0051418 GO:0051493 GO:0051495 GO:0051704 GO:0051707 GO:0055028 GO:0065003 GO:0065007 GO:0070507 GO:0070925 GO:0071840 GO:0071944 GO:0090063 GO:0090307 GO:0097435 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1902850 GO:1902903 GO:1902905 GO:1903047
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spc97_Spc98

Protein Analysis

320

Amino Acids

34.68

Weight (kDa)

10.52

Isoelectric Point (pI)

50.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 484, 577
AccIII TCCGGA 1 cut(s) 728
AclWI GGATC 4 cut(s) 216, 287, 821, 834
AcoI YGGCCR 2 cut(s) 472, 701
AcsI RAATTY 1 cut(s) 223
AcvI CACGTG 1 cut(s) 598
AcyI GRCGYC 3 cut(s) 42, 578, 745
AfaI GTAC 1 cut(s) 754
AfiI CCNNNNNNNGG 9 cut(s) 261, 384, 385, 481, 538, 699, 740, 767, 864
AflII CTTAAG 1 cut(s) 191
AgsI TTSAA 2 cut(s) 7, 15
AluBI AGCT 3 cut(s) 526, 792, 870
AluI AGCT 3 cut(s) 526, 792, 870
Alw26I GTCTC 1 cut(s) 449
AlwI GGATC 4 cut(s) 216, 287, 821, 834
Ama87I CYCGRG 6 cut(s) 228, 549, 587, 740, 822, 841
Aor13HI TCCGGA 1 cut(s) 728
AoxI GGCC 5 cut(s) 148, 375, 472, 553, 701
ApaI GGGCCC 1 cut(s) 152
ApeKI GCWGC 5 cut(s) 331, 334, 438, 458, 764
ApoI RAATTY 1 cut(s) 223
AspLEI GCGC 1 cut(s) 580
AspS9I GGNCC 6 cut(s) 148, 149, 376, 388, 554, 731
AsuC2I CCSGG 5 cut(s) 38, 700, 741, 742, 761
AsuHPI GGTGA 3 cut(s) 25, 688, 959
AvaI CYCGRG 6 cut(s) 228, 549, 587, 740, 822, 841
AvaII GGWCC 2 cut(s) 388, 731
BaeGI GKGCMC 2 cut(s) 152, 603
BamHI GGATCC 1 cut(s) 826
BanI GGYRCC 2 cut(s) 484, 577
BanII GRGCYC 1 cut(s) 152
BbrPI CACGTG 1 cut(s) 598
BbvI GCAGC 5 cut(s) 318, 346, 425, 445, 776
BccI CCATC 1 cut(s) 59
BceAI ACGGC 2 cut(s) 677, 683
BcgI CGANNNNNNTGC 4 cut(s) 886, 901, 920, 935
BcnI CCSGG 5 cut(s) 38, 700, 741, 742, 761
BcoDI GTCTC 1 cut(s) 449
BfaI CTAG 2 cut(s) 582, 671
BfmI CTRYAG 2 cut(s) 332, 439
BfoI RGCGCY 1 cut(s) 581
BfrI CTTAAG 1 cut(s) 191
Bme1390I CCNGG 5 cut(s) 38, 700, 741, 742, 761
Bme18I GGWCC 2 cut(s) 388, 731
BmeT110I CYCGRG 6 cut(s) 228, 549, 587, 740, 822, 841
BmgT120I GGNCC 6 cut(s) 148, 149, 376, 388, 554, 731
BmiI GGNNCC 9 cut(s) 150, 151, 389, 486, 579, 678, 798, 828, 856
BmrFI CCNGG 5 cut(s) 38, 700, 741, 742, 761
BmrI ACTGGG 1 cut(s) 855
BmsI GCATC 1 cut(s) 146
BmuI ACTGGG 1 cut(s) 855
BoxI GACNNNNGTC 1 cut(s) 512
BpuEI CTTGAG 1 cut(s) 69
BpuMI CCSGG 5 cut(s) 38, 700, 741, 742, 761
BsaAI YACGTR 1 cut(s) 598
BsaHI GRCGYC 3 cut(s) 42, 578, 745
BsaJI CCNNGG 4 cut(s) 36, 550, 740, 842
BsaWI WCCGGW 1 cut(s) 728
Bsc4I CCNNNNNNNGG 9 cut(s) 261, 384, 385, 481, 538, 699, 740, 767, 864
Bse118I RCCGGY 4 cut(s) 481, 538, 767, 879
Bse1I ACTGG 1 cut(s) 861
Bse3DI GCAATG 2 cut(s) 344, 453
BseAI TCCGGA 1 cut(s) 728
BseDI CCNNGG 4 cut(s) 36, 550, 740, 842
BseGI GGATG 2 cut(s) 137, 736
BseLI CCNNNNNNNGG 9 cut(s) 261, 384, 385, 481, 538, 699, 740, 767, 864
BseMI GCAATG 2 cut(s) 344, 453
BseNI ACTGG 1 cut(s) 861
BseRI GAGGAG 2 cut(s) 480, 689
BseSI GKGCMC 2 cut(s) 152, 603
BseX3I CGGCCG 1 cut(s) 701
BseXI GCAGC 5 cut(s) 318, 346, 425, 445, 776
BseYI CCCAGC 1 cut(s) 713
Bsh1285I CGRYCG 1 cut(s) 704
BshFI GGCC 5 cut(s) 150, 377, 474, 555, 703
BshNI GGYRCC 2 cut(s) 484, 577
BsiEI CGRYCG 1 cut(s) 704
BsiHKCI CYCGRG 6 cut(s) 228, 549, 587, 740, 822, 841
BslFI GGGAC 4 cut(s) 577, 627, 743, 830
BslI CCNNNNNNNGG 9 cut(s) 261, 384, 385, 481, 538, 699, 740, 767, 864
BsmAI GTCTC 1 cut(s) 449
BsmFI GGGAC 4 cut(s) 577, 627, 743, 830
BsnI GGCC 5 cut(s) 150, 377, 474, 555, 703
BsoBI CYCGRG 6 cut(s) 228, 549, 587, 740, 822, 841
Bsp120I GGGCCC 1 cut(s) 148
Bsp1286I GDGCHC 2 cut(s) 152, 603
Bsp13I TCCGGA 1 cut(s) 728
Bsp143I GATC 3 cut(s) 208, 279, 826
BspANI GGCC 5 cut(s) 150, 377, 474, 555, 703
BspEI TCCGGA 1 cut(s) 728
BspLI GGNNCC 9 cut(s) 150, 151, 389, 486, 579, 678, 798, 828, 856
BspMAI CTGCAG 2 cut(s) 336, 443
BspPI GGATC 4 cut(s) 216, 287, 821, 834
BspT107I GGYRCC 2 cut(s) 484, 577
BspTI CTTAAG 1 cut(s) 191
BsrDI GCAATG 2 cut(s) 344, 453
BsrFI RCCGGY 4 cut(s) 481, 538, 767, 879
BsrI ACTGG 1 cut(s) 861
BssAI RCCGGY 4 cut(s) 481, 538, 767, 879
BssECI CCNNGG 4 cut(s) 36, 550, 740, 842
BssMI GATC 3 cut(s) 208, 279, 826
BssNI GRCGYC 3 cut(s) 42, 578, 745
Bst4CI ACNGT 2 cut(s) 757, 947
Bst6I CTCTTC 1 cut(s) 366
BstACI GRCGYC 3 cut(s) 42, 578, 745
BstAFI CTTAAG 1 cut(s) 191
BstBAI YACGTR 1 cut(s) 598
BstC8I GCNNGC 4 cut(s) 540, 603, 769, 881
BstDEI CTNAG 2 cut(s) 924, 960
BstF5I GGATG 2 cut(s) 137, 736
BstH2I RGCGCY 1 cut(s) 581
BstHHI GCGC 1 cut(s) 580
BstKTI GATC 3 cut(s) 211, 282, 829
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 3 cut(s) 208, 279, 826
BstMCI CGRYCG 1 cut(s) 704
BstMWI GCNNNNNNNGC 5 cut(s) 53, 544, 654, 676, 876
BstPAI GACNNNNGTC 1 cut(s) 512
BstSCI CCNGG 5 cut(s) 36, 698, 739, 740, 759
BstSFI CTRYAG 2 cut(s) 332, 439
BstSLI GKGCMC 2 cut(s) 152, 603
BstV1I GCAGC 5 cut(s) 318, 346, 425, 445, 776
BstX2I RGATCY 1 cut(s) 826
BstYI RGATCY 1 cut(s) 826
BstZI CGGCCG 1 cut(s) 701
BsuRI GGCC 5 cut(s) 150, 377, 474, 555, 703
BtgZI GCGATG 1 cut(s) 37
BtsCI GGATG 2 cut(s) 137, 736
BtsIMutI CAGTG 1 cut(s) 868
Cac8I GCNNGC 4 cut(s) 540, 603, 769, 881
CfoI GCGC 1 cut(s) 580
Cfr10I RCCGGY 4 cut(s) 481, 538, 767, 879
Cfr13I GGNCC 6 cut(s) 148, 149, 376, 388, 554, 731
Cfr9I CCCGGG 1 cut(s) 740
CseI GACGC 3 cut(s) 50, 734, 827
Csp6I GTAC 1 cut(s) 753
CviAII CATG 3 cut(s) 476, 505, 533
CviQI GTAC 1 cut(s) 753
DdeI CTNAG 2 cut(s) 924, 960
DinI GGCGCC 1 cut(s) 579
DpnI GATC 3 cut(s) 210, 281, 828
DpnII GATC 3 cut(s) 208, 279, 826
DraI TTTAAA 1 cut(s) 268
EaeI YGGCCR 2 cut(s) 472, 701
EagI CGGCCG 1 cut(s) 701
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
EciI GGCGGA 1 cut(s) 518
EclXI CGGCCG 1 cut(s) 701
Eco24I GRGCYC 1 cut(s) 152
Eco47I GGWCC 2 cut(s) 388, 731
Eco52I CGGCCG 1 cut(s) 701
Eco72I CACGTG 1 cut(s) 598
Eco88I CYCGRG 6 cut(s) 228, 549, 587, 740, 822, 841
EcoO109I RGGNCCY 1 cut(s) 149
EcoRI GAATTC 1 cut(s) 223
EcoT38I GRGCYC 1 cut(s) 152
EgeI GGCGCC 1 cut(s) 579
EheI GGCGCC 1 cut(s) 579
FaeI CATG 3 cut(s) 479, 508, 536
FaqI GGGAC 4 cut(s) 577, 627, 743, 830
FatI CATG 3 cut(s) 475, 504, 532
FauI CCCGC 3 cut(s) 502, 610, 653
FokI GGATG 2 cut(s) 124, 723
FriOI GRGCYC 1 cut(s) 152
FspBI CTAG 2 cut(s) 582, 671
GlaI GCGC 1 cut(s) 579
GsaI CCCAGC 1 cut(s) 717
HaeII RGCGCY 1 cut(s) 581
HaeIII GGCC 5 cut(s) 150, 377, 474, 555, 703
HgaI GACGC 3 cut(s) 50, 734, 827
HhaI GCGC 1 cut(s) 580
Hin1I GRCGYC 3 cut(s) 42, 578, 745
Hin1II CATG 3 cut(s) 479, 508, 536
Hin6I GCGC 1 cut(s) 578
HinP1I GCGC 1 cut(s) 578
HinfI GANTC 7 cut(s) 59, 75, 237, 291, 467, 590, 898
HphI GGTGA 3 cut(s) 25, 688, 959
Hpy166II GTNNAC 2 cut(s) 63, 406
Hpy188I TCNGA 4 cut(s) 205, 279, 854, 912
Hpy188III TCNNGA 5 cut(s) 15, 228, 729, 824, 830
Hpy8I GTNNAC 2 cut(s) 63, 406
Hpy99I CGWCG 2 cut(s) 279, 750
HpyAV CCTTC 3 cut(s) 304, 378, 574
HpyCH4III ACNGT 2 cut(s) 757, 947
HpyCH4IV ACGT 2 cut(s) 274, 597
HpyCH4V TGCA 4 cut(s) 334, 441, 453, 937
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 544, 654, 676, 876
HpyF3I CTNAG 2 cut(s) 924, 960
HpySE526I ACGT 2 cut(s) 274, 597
Hsp92I GRCGYC 3 cut(s) 42, 578, 745
Hsp92II CATG 3 cut(s) 479, 508, 536
HspAI GCGC 1 cut(s) 578
KasI GGCGCC 1 cut(s) 577
Kpn2I TCCGGA 1 cut(s) 728
KroI GCCGGC 3 cut(s) 538, 767, 879
KroNI GCCGGC 3 cut(s) 540, 769, 881
Kzo9I GATC 3 cut(s) 208, 279, 826
LmnI GCTCC 4 cut(s) 531, 676, 789, 867
Lsp1109I GCAGC 5 cut(s) 318, 346, 425, 445, 776
LweI GCATC 1 cut(s) 146
MaeI CTAG 2 cut(s) 582, 671
MaeII ACGT 2 cut(s) 274, 597
MaeIII GTNAC 4 cut(s) 31, 694, 947, 955
MalI GATC 3 cut(s) 210, 281, 828
MboI GATC 3 cut(s) 208, 279, 826
MboII GAAGA 2 cut(s) 232, 383
MflI RGATCY 1 cut(s) 826
MhlI GDGCHC 2 cut(s) 152, 603
MluCI AATT 2 cut(s) 223, 257
Mly113I GGCGCC 1 cut(s) 578
MlyI GAGTC 6 cut(s) 68, 69, 300, 461, 599, 907
MmeI TCCRAC 2 cut(s) 214, 257
MreI CGCCGGCG 1 cut(s) 879
MroI TCCGGA 1 cut(s) 728
MroNI GCCGGC 3 cut(s) 538, 767, 879
MseI TTAA 3 cut(s) 192, 267, 522
MspCI CTTAAG 1 cut(s) 191
MspR9I CCNGG 5 cut(s) 38, 700, 741, 742, 761
MssI GTTTAAAC 1 cut(s) 268
MwoI GCNNNNNNNGC 5 cut(s) 53, 544, 654, 676, 876
NaeI GCCGGC 3 cut(s) 540, 769, 881
NarI GGCGCC 1 cut(s) 578
NciI CCSGG 5 cut(s) 38, 700, 741, 742, 761
NdeII GATC 3 cut(s) 208, 279, 826
NgoMIV GCCGGC 3 cut(s) 538, 767, 879
NlaIII CATG 3 cut(s) 479, 508, 536
NlaIV GGNNCC 9 cut(s) 150, 151, 389, 486, 579, 678, 798, 828, 856
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 3 cut(s) 31, 694, 947
PdiI GCCGGC 3 cut(s) 540, 769, 881
PfeI GAWTC 1 cut(s) 237
PleI GAGTC 6 cut(s) 67, 69, 299, 461, 598, 906
PluTI GGCGCC 1 cut(s) 581
PmaCI CACGTG 1 cut(s) 598
PmeI GTTTAAAC 1 cut(s) 268
PmlI CACGTG 1 cut(s) 598
PpsI GAGTC 6 cut(s) 67, 69, 299, 461, 598, 906
Ppu21I YACGTR 1 cut(s) 598
PshAI GACNNNNGTC 1 cut(s) 512
PspCI CACGTG 1 cut(s) 598
PspFI CCCAGC 1 cut(s) 713
PspN4I GGNNCC 9 cut(s) 150, 151, 389, 486, 579, 678, 798, 828, 856
PspOMI GGGCCC 1 cut(s) 148
PspPI GGNCC 6 cut(s) 148, 149, 376, 388, 554, 731
PstI CTGCAG 2 cut(s) 336, 443
PsuI RGATCY 1 cut(s) 826
RsaI GTAC 1 cut(s) 754
RsaNI GTAC 1 cut(s) 753
SaqAI TTAA 3 cut(s) 192, 267, 522
Sau3AI GATC 3 cut(s) 208, 279, 826
Sau96I GGNCC 6 cut(s) 148, 149, 376, 388, 554, 731
SchI GAGTC 6 cut(s) 68, 69, 300, 461, 599, 907
ScrFI CCNGG 5 cut(s) 38, 700, 741, 742, 761
SduI GDGCHC 2 cut(s) 152, 603
SfaNI GCATC 1 cut(s) 146
SfcI CTRYAG 2 cut(s) 332, 439
SfoI GGCGCC 1 cut(s) 579
SgrAI CRCCGGYG 1 cut(s) 879
SinI GGWCC 2 cut(s) 388, 731
SmaI CCCGGG 1 cut(s) 742
SmlI CTYRAG 2 cut(s) 84, 191
SmoI CTYRAG 2 cut(s) 84, 191
Sse9I AATT 2 cut(s) 223, 257
SspDI GGCGCC 1 cut(s) 577
SspMI CTAG 2 cut(s) 582, 671
StyD4I CCNGG 5 cut(s) 36, 698, 739, 740, 759
TaaI ACNGT 2 cut(s) 757, 947
TaiI ACGT 2 cut(s) 277, 600
TaqI TCGA 3 cut(s) 211, 294, 748
TasI AATT 2 cut(s) 223, 257
TauI GCSGC 6 cut(s) 47, 50, 289, 578, 682, 706
TfiI GAWTC 1 cut(s) 237
Tru1I TTAA 3 cut(s) 192, 267, 522
Tru9I TTAA 3 cut(s) 192, 267, 522
TscAI CASTG 1 cut(s) 868
TseFI GTSAC 3 cut(s) 31, 694, 947
TseI GCWGC 5 cut(s) 331, 334, 438, 458, 764
Tsp45I GTSAC 3 cut(s) 31, 694, 947
TspDTI ATGAA 1 cut(s) 492
TspGWI ACGGA 2 cut(s) 19, 665
TspMI CCCGGG 1 cut(s) 740
TspRI CASTG 1 cut(s) 868
Vha464I CTTAAG 1 cut(s) 191
VpaK11BI GGWCC 2 cut(s) 388, 731
XapI RAATTY 1 cut(s) 223
XmaI CCCGGG 1 cut(s) 740
XspI CTAG 2 cut(s) 582, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.