Rmu_sc0003116.1_g000011

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003116.1
OG0010703
Physical Location & Seq
Forward (+)
57680 .. 60305
2626 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1083 bp
atgttggctttttgggttgctttggtcctgctggttgcttgtgctggtcgtcaagccgaggcacgagcattctttgttttcggagattcgctggtggacaatggcaacaatgactacttggtcaccactgcccgggccgactcgtacccttatgggatcgactacccgactcacataccaaccggacggttttctaatggcctcaacataccagacctcatcagtgagtcaattgggtcagaacccacattgccatacttgagtcctcacctcactggacaaaacctacttgttggtgccaactttgcttctgctgggattggaatcctcaatgacactggaatccagtttcttaacataatcagaatcaccaagcaactggaatacttcgaacaataccagacaagggtgagtgccctaatcggacctgatcagactcaaagccttgtgaacggagccctggtccttataacccttggtggaaatgactttgtaaacaactactacttggttccctactctgctaggtctcgccaatactctctcccagactacgtcgtctacctcatctctgagtatcgcaaaatcctcaacaggctgtacgaactgggagcccgtcaagtcctggtcacaggcacaggaccactaggctgtgtccccgccgagcttgccatgcatagccgaaacggggagtgctccgtggagctccaacgcgctgcctcgctcttcaacccgcagctagtccaaatgatcagctcgctcaacagcgatctcgggtccaacgtcttcattgccgcaaacgcctaccaaatgcacatggatttcgttagtaaccccgctgcctacgggtttgtaacatcaaagatagcctgttgtggccaaggaccttacaatgggatcggactatgcacacccctttcgaacctgtgcccgaaccgagacttgtacgcgttttgggatccgttccatccttcggagaaggctaataggatcattgtgcagcaaatcctgactgggaccgaagagtacatgcaccccatgaacctcagtactatgttggccttggacaactctaggacctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0008150 GO:0032501 GO:0032502 GO:0042335 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

360

Amino Acids

39.53

Weight (kDa)

5.21

Isoelectric Point (pI)

35.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 25 - 335 1.2e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010703)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04290 AT5G33370 AT5G33370
fragaria_vesca FvH4_6g01390 FvH4_6g01390
malus_domestica MD04G1234300.v1.1 MD12G1252200.v1.1
prunus_persica Prupe.6G354100_v2.0.a1
pyrus_communis pycom04g20800
rosa_chinensis RchiOBHm_Chr3g0448981 RchiOBHm_Chr3g0449021
rosa_laevigata RLG00000025862
rosa_multiflora Rmu_sc0003116.1_g000011
rosa_roxburghii Rroxscaffold_6G00427140
rosa_rugosa Rorug02G0617400
rosa_samantha Rh3AG015900 Rh3BG016000 Rh3CG015000 Rh3DG016300
rosa_wichuraiana Rw3G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 470
AasI GACNNNNNNGTC 2 cut(s) 119, 557
AccB1I GGYRCC 1 cut(s) 296
AccI GTMKAC 1 cut(s) 561
AccII CGCG 2 cut(s) 714, 950
AciI CCGC 4 cut(s) 660, 734, 795, 837
AclWI GGATC 5 cut(s) 164, 905, 953, 966, 998
AcoI YGGCCR 1 cut(s) 877
AfaI GTAC 5 cut(s) 146, 602, 947, 1028, 1051
AfiI CCNNNNNNNGG 5 cut(s) 132, 406, 688, 893, 973
AflIII ACRYGT 1 cut(s) 948
AgsI TTSAA 1 cut(s) 730
AjnI CCWGG 2 cut(s) 459, 624
AluBI AGCT 4 cut(s) 667, 706, 739, 756
AluI AGCT 4 cut(s) 667, 706, 739, 756
Alw21I GWGCWC 2 cut(s) 698, 708
Alw26I GTCTC 2 cut(s) 534, 933
AlwI GGATC 5 cut(s) 164, 905, 953, 966, 998
Ama87I CYCGRG 2 cut(s) 132, 773
AoxI GGCC 4 cut(s) 135, 199, 877, 1059
ApeKI GCWGC 4 cut(s) 716, 736, 839, 1000
AspLEI GCGC 1 cut(s) 716
AspS9I GGNCC 9 cut(s) 25, 135, 425, 463, 641, 777, 884, 1017, 1077
AsuC2I CCSGG 2 cut(s) 133, 134
AsuHPI GGTGA 4 cut(s) 115, 260, 361, 421
AsuII TTCGAA 2 cut(s) 390, 920
AvaI CYCGRG 2 cut(s) 132, 773
AvaII GGWCC 8 cut(s) 25, 425, 463, 641, 777, 884, 1017, 1077
BaeGI GKGCMC 2 cut(s) 418, 932
BalI TGGCCA 1 cut(s) 879
BamHI GGATCC 1 cut(s) 958
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 3 cut(s) 460, 616, 708
BauI CACGAG 1 cut(s) 63
BbsI GAAGAC 1 cut(s) 778
Bbv12I GWGCWC 2 cut(s) 698, 708
BbvI GCAGC 4 cut(s) 703, 748, 826, 1012
BccI CCATC 1 cut(s) 975
BciT130I CCWGG 2 cut(s) 461, 626
BclI TGATCA 2 cut(s) 430, 750
BcnI CCSGG 2 cut(s) 133, 134
BcoDI GTCTC 2 cut(s) 534, 933
BfaI CTAG 5 cut(s) 525, 647, 740, 1074, 1081
BisI GCNGC 5 cut(s) 717, 737, 795, 840, 1001
BlsI GCNGC 5 cut(s) 718, 738, 796, 841, 1002
BmcAI AGTACT 1 cut(s) 1051
Bme1390I CCNGG 4 cut(s) 133, 134, 461, 626
Bme18I GGWCC 8 cut(s) 25, 425, 463, 641, 777, 884, 1017, 1077
BmeT110I CYCGRG 2 cut(s) 132, 773
BmgT120I GGNCC 9 cut(s) 25, 135, 425, 463, 641, 777, 884, 1017, 1077
BmiI GGNNCC 7 cut(s) 298, 457, 513, 613, 778, 960, 1018
BmrFI CCNGG 4 cut(s) 133, 134, 461, 626
BmrI ACTGGG 2 cut(s) 617, 1023
BmuI ACTGGG 2 cut(s) 617, 1023
BpiI GAAGAC 1 cut(s) 778
Bpu14I TTCGAA 2 cut(s) 390, 920
BpuEI CTTGAG 1 cut(s) 280
BpuMI CCSGG 2 cut(s) 133, 134
BsaBI GATNNNNATC 1 cut(s) 323
BsaI GGTCTC 1 cut(s) 534
BsaJI CCNNGG 7 cut(s) 57, 132, 459, 475, 699, 880, 1062
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 2 cut(s) 447, 477
Bsc4I CCNNNNNNNGG 5 cut(s) 132, 406, 688, 893, 973
Bse1I ACTGG 6 cut(s) 280, 343, 346, 384, 612, 1018
Bse3DI GCAATG 2 cut(s) 248, 789
Bse8I GATNNNNATC 1 cut(s) 323
BseBI CCWGG 2 cut(s) 461, 626
BseDI CCNNGG 7 cut(s) 57, 132, 459, 475, 699, 880, 1062
BseGI GGATG 1 cut(s) 967
BseJI GATNNNNATC 1 cut(s) 323
BseLI CCNNNNNNNGG 5 cut(s) 132, 406, 688, 893, 973
BseMI GCAATG 2 cut(s) 248, 789
BseMII CTCAG 2 cut(s) 564, 1060
BseNI ACTGG 6 cut(s) 280, 343, 346, 384, 612, 1018
BseSI GKGCMC 2 cut(s) 418, 932
BseXI GCAGC 4 cut(s) 703, 748, 826, 1012
BseYI CCCAGC 1 cut(s) 314
BsgI GTGCAG 1 cut(s) 1019
Bsh1236I CGCG 2 cut(s) 714, 950
BshFI GGCC 4 cut(s) 137, 201, 879, 1061
BshNI GGYRCC 1 cut(s) 296
BsiHKAI GWGCWC 2 cut(s) 698, 708
BsiHKCI CYCGRG 2 cut(s) 132, 773
BsiSI CCGG 2 cut(s) 133, 183
BslFI GGGAC 2 cut(s) 641, 1030
BslI CCNNNNNNNGG 5 cut(s) 132, 406, 688, 893, 973
BsmAI GTCTC 2 cut(s) 534, 933
BsmFI GGGAC 2 cut(s) 641, 1030
BsmI GAATGC 1 cut(s) 68
BsnI GGCC 4 cut(s) 137, 201, 879, 1061
Bso31I GGTCTC 1 cut(s) 534
BsoBI CYCGRG 2 cut(s) 132, 773
Bsp119I TTCGAA 2 cut(s) 390, 920
Bsp1286I GDGCHC 6 cut(s) 418, 460, 616, 698, 708, 932
Bsp143I GATC 7 cut(s) 156, 430, 750, 769, 897, 958, 990
BspACI CCGC 4 cut(s) 660, 734, 795, 837
BspANI GGCC 4 cut(s) 137, 201, 879, 1061
BspCNI CTCAG 2 cut(s) 565, 1059
BspFNI CGCG 2 cut(s) 714, 950
BspLI GGNNCC 7 cut(s) 298, 457, 513, 613, 778, 960, 1018
BspPI GGATC 5 cut(s) 164, 905, 953, 966, 998
BspQI GCTCTTC 1 cut(s) 731
BspT104I TTCGAA 2 cut(s) 390, 920
BspT107I GGYRCC 1 cut(s) 296
BspTNI GGTCTC 1 cut(s) 534
BsrDI GCAATG 2 cut(s) 248, 789
BsrI ACTGG 6 cut(s) 280, 343, 346, 384, 612, 1018
BssECI CCNNGG 7 cut(s) 57, 132, 459, 475, 699, 880, 1062
BssMI GATC 7 cut(s) 156, 430, 750, 769, 897, 958, 990
BssSI CACGAG 1 cut(s) 63
BssT1I CCWWGG 3 cut(s) 475, 880, 1062
Bst2BI CACGAG 1 cut(s) 63
Bst2UI CCWGG 2 cut(s) 461, 626
Bst4CI ACNGT 1 cut(s) 189
Bst6I CTCTTC 2 cut(s) 731, 1017
BstBI TTCGAA 2 cut(s) 390, 920
BstC8I GCNNGC 2 cut(s) 669, 758
BstDEI CTNAG 2 cut(s) 573, 1046
BstDSI CCRYGG 1 cut(s) 699
BstEII GGTNACC 1 cut(s) 121
BstF5I GGATG 1 cut(s) 967
BstFNI CGCG 2 cut(s) 714, 950
BstHHI GCGC 1 cut(s) 716
BstKTI GATC 7 cut(s) 159, 433, 753, 772, 900, 961, 993
BstMAI GTCTC 2 cut(s) 534, 933
BstMBI GATC 7 cut(s) 156, 430, 750, 769, 897, 958, 990
BstMWI GCNNNNNNNGC 4 cut(s) 305, 668, 673, 800
BstNI CCWGG 2 cut(s) 461, 626
BstNSI RCATGY 1 cut(s) 1033
BstPI GGTNACC 1 cut(s) 121
BstSCI CCNGG 4 cut(s) 131, 132, 459, 624
BstSLI GKGCMC 2 cut(s) 418, 932
BstUI CGCG 2 cut(s) 714, 950
BstV1I GCAGC 4 cut(s) 703, 748, 826, 1012
BstV2I GAAGAC 1 cut(s) 778
BstX2I RGATCY 1 cut(s) 958
BstXI CCANNNNNNTGG 1 cut(s) 379
BstYI RGATCY 1 cut(s) 958
BsuRI GGCC 4 cut(s) 137, 201, 879, 1061
BtgI CCRYGG 1 cut(s) 699
BtsCI GGATG 1 cut(s) 967
BtsI GCAGTG 1 cut(s) 126
BtsIMutI CAGTG 4 cut(s) 126, 229, 273, 336
Cac8I GCNNGC 2 cut(s) 669, 758
CfoI GCGC 1 cut(s) 716
Cfr13I GGNCC 9 cut(s) 25, 135, 425, 463, 641, 777, 884, 1017, 1077
Cfr9I CCCGGG 1 cut(s) 132
Csp6I GTAC 5 cut(s) 145, 601, 946, 1027, 1050
CviAII CATG 4 cut(s) 673, 817, 1030, 1039
CviQI GTAC 5 cut(s) 145, 601, 946, 1027, 1050
DdeI CTNAG 2 cut(s) 573, 1046
DpnI GATC 7 cut(s) 158, 432, 752, 771, 899, 960, 992
DpnII GATC 7 cut(s) 156, 430, 750, 769, 897, 958, 990
DrdI GACNNNNNNGTC 2 cut(s) 119, 557
DseDI GACNNNNNNGTC 2 cut(s) 119, 557
EaeI YGGCCR 1 cut(s) 877
Eam1104I CTCTTC 2 cut(s) 731, 1017
EarI CTCTTC 2 cut(s) 731, 1017
Ecl136II GAGCTC 1 cut(s) 706
Eco130I CCWWGG 3 cut(s) 475, 880, 1062
Eco24I GRGCYC 3 cut(s) 460, 616, 708
Eco31I GGTCTC 1 cut(s) 534
Eco47I GGWCC 8 cut(s) 25, 425, 463, 641, 777, 884, 1017, 1077
Eco53kI GAGCTC 1 cut(s) 706
Eco88I CYCGRG 2 cut(s) 132, 773
Eco91I GGTNACC 1 cut(s) 121
EcoICRI GAGCTC 1 cut(s) 706
EcoO109I RGGNCCY 2 cut(s) 884, 1077
EcoO65I GGTNACC 1 cut(s) 121
EcoRII CCWGG 2 cut(s) 459, 624
EcoT14I CCWWGG 3 cut(s) 475, 880, 1062
EcoT22I ATGCAT 1 cut(s) 678
EcoT38I GRGCYC 3 cut(s) 460, 616, 708
ErhI CCWWGG 3 cut(s) 475, 880, 1062
FaeI CATG 4 cut(s) 676, 820, 1033, 1042
FaqI GGGAC 2 cut(s) 641, 1030
FatI CATG 4 cut(s) 672, 816, 1029, 1038
FauI CCCGC 3 cut(s) 667, 741, 844
FbaI TGATCA 2 cut(s) 430, 750
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 5 cut(s) 717, 737, 795, 840, 1001
FokI GGATG 1 cut(s) 954
FriOI GRGCYC 3 cut(s) 460, 616, 708
Fsp4HI GCNGC 5 cut(s) 717, 737, 795, 840, 1001
FspBI CTAG 5 cut(s) 525, 647, 740, 1074, 1081
GlaI GCGC 1 cut(s) 715
GluI GCNGC 5 cut(s) 717, 737, 795, 840, 1001
GsaI CCCAGC 1 cut(s) 318
HaeIII GGCC 4 cut(s) 137, 201, 879, 1061
HapII CCGG 2 cut(s) 133, 183
HhaI GCGC 1 cut(s) 716
Hin1II CATG 4 cut(s) 676, 820, 1033, 1042
Hin6I GCGC 1 cut(s) 714
HinP1I GCGC 1 cut(s) 714
HinfI GANTC 9 cut(s) 86, 140, 169, 227, 262, 324, 342, 366, 436
HpaII CCGG 2 cut(s) 133, 183
HphI GGTGA 4 cut(s) 115, 260, 361, 421
Hpy166II GTNNAC 4 cut(s) 97, 451, 496, 562
Hpy188I TCNGA 8 cut(s) 83, 241, 365, 425, 435, 574, 902, 976
Hpy188III TCNNGA 1 cut(s) 1009
Hpy8I GTNNAC 4 cut(s) 97, 451, 496, 562
Hpy99I CGWCG 1 cut(s) 560
HpyAV CCTTC 2 cut(s) 973, 981
HpyCH4III ACNGT 1 cut(s) 189
HpyCH4IV ACGT 2 cut(s) 555, 783
HpyCH4V TGCA 5 cut(s) 676, 814, 909, 1000, 1033
HpyF10VI GCNNNNNNNGC 4 cut(s) 305, 668, 673, 800
HpyF3I CTNAG 2 cut(s) 573, 1046
HpySE526I ACGT 2 cut(s) 555, 783
Hsp92II CATG 4 cut(s) 676, 820, 1033, 1042
HspAI GCGC 1 cut(s) 714
Ksp22I TGATCA 2 cut(s) 430, 750
Kzo9I GATC 7 cut(s) 156, 430, 750, 769, 897, 958, 990
LguI GCTCTTC 1 cut(s) 731
LmnI GCTCC 5 cut(s) 455, 611, 701, 703, 711
Lsp1109I GCAGC 4 cut(s) 703, 748, 826, 1012
MaeI CTAG 5 cut(s) 525, 647, 740, 1074, 1081
MaeII ACGT 2 cut(s) 555, 783
MaeIII GTNAC 4 cut(s) 121, 628, 830, 853
MalI GATC 7 cut(s) 158, 432, 752, 771, 899, 960, 992
MboI GATC 7 cut(s) 156, 430, 750, 769, 897, 958, 990
MboII GAAGA 3 cut(s) 718, 778, 1034
MfeI CAATTG 1 cut(s) 231
MflI RGATCY 1 cut(s) 958
MhlI GDGCHC 6 cut(s) 418, 460, 616, 698, 708, 932
MlsI TGGCCA 1 cut(s) 879
MluCI AATT 1 cut(s) 231
MluI ACGCGT 1 cut(s) 948
MluNI TGGCCA 1 cut(s) 879
MlyI GAGTC 5 cut(s) 134, 163, 236, 271, 430
MmeI TCCRAC 2 cut(s) 733, 804
Mox20I TGGCCA 1 cut(s) 879
Mph1103I ATGCAT 1 cut(s) 678
MscI TGGCCA 1 cut(s) 879
MseI TTAA 1 cut(s) 354
Msp20I TGGCCA 1 cut(s) 879
MspA1I CMGCKG 1 cut(s) 839
MspI CCGG 2 cut(s) 133, 183
MspR9I CCNGG 4 cut(s) 133, 134, 461, 626
MunI CAATTG 1 cut(s) 231
Mva1269I GAATGC 1 cut(s) 68
MvaI CCWGG 2 cut(s) 461, 626
MvnI CGCG 2 cut(s) 714, 950
MwoI GCNNNNNNNGC 4 cut(s) 305, 668, 673, 800
NciI CCSGG 2 cut(s) 133, 134
NdeII GATC 7 cut(s) 156, 430, 750, 769, 897, 958, 990
NlaIII CATG 4 cut(s) 676, 820, 1033, 1042
NlaIV GGNNCC 7 cut(s) 298, 457, 513, 613, 778, 960, 1018
NmeAIII GCCGAG 2 cut(s) 82, 688
NmuCI GTSAC 2 cut(s) 121, 628
NsiI ATGCAT 1 cut(s) 678
NspI RCATGY 1 cut(s) 1033
NspV TTCGAA 2 cut(s) 390, 920
PciSI GCTCTTC 1 cut(s) 731
PcsI WCGNNNNNNNCGW 1 cut(s) 780
PctI GAATGC 1 cut(s) 68
PfeI GAWTC 4 cut(s) 86, 324, 342, 366
PflFI GACNNNGTC 1 cut(s) 554
PkrI GCNGC 5 cut(s) 718, 738, 796, 841, 1002
PleI GAGTC 5 cut(s) 134, 163, 235, 270, 430
PpsI GAGTC 5 cut(s) 134, 163, 235, 270, 430
PpuMI RGGWCCY 2 cut(s) 884, 1077
PsiI TTATAA 1 cut(s) 470
Psp124BI GAGCTC 1 cut(s) 708
Psp5II RGGWCCY 2 cut(s) 884, 1077
Psp6I CCWGG 2 cut(s) 459, 624
PspEI GGTNACC 1 cut(s) 121
PspFI CCCAGC 1 cut(s) 314
PspGI CCWGG 2 cut(s) 459, 624
PspN4I GGNNCC 7 cut(s) 298, 457, 513, 613, 778, 960, 1018
PspPI GGNCC 9 cut(s) 25, 135, 425, 463, 641, 777, 884, 1017, 1077
PspPPI RGGWCCY 2 cut(s) 884, 1077
PsuI RGATCY 1 cut(s) 958
PsyI GACNNNGTC 1 cut(s) 554
RsaI GTAC 5 cut(s) 146, 602, 947, 1028, 1051
RsaNI GTAC 5 cut(s) 145, 601, 946, 1027, 1050
SacI GAGCTC 1 cut(s) 708
SapI GCTCTTC 1 cut(s) 731
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 5 cut(s) 717, 737, 795, 840, 1001
Sau3AI GATC 7 cut(s) 156, 430, 750, 769, 897, 958, 990
Sau96I GGNCC 9 cut(s) 25, 135, 425, 463, 641, 777, 884, 1017, 1077
ScaI AGTACT 1 cut(s) 1051
SchI GAGTC 5 cut(s) 134, 163, 236, 271, 430
ScrFI CCNGG 4 cut(s) 133, 134, 461, 626
SduI GDGCHC 6 cut(s) 418, 460, 616, 698, 708, 932
SfuI TTCGAA 2 cut(s) 390, 920
SinI GGWCC 8 cut(s) 25, 425, 463, 641, 777, 884, 1017, 1077
SmaI CCCGGG 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 259
SmoI CTYRAG 1 cut(s) 259
SrfI GCCCGGGC 1 cut(s) 134
Sse9I AATT 1 cut(s) 231
SsiI CCGC 4 cut(s) 660, 734, 795, 837
SspMI CTAG 5 cut(s) 525, 647, 740, 1074, 1081
SstI GAGCTC 1 cut(s) 708
StyD4I CCNGG 4 cut(s) 131, 132, 459, 624
StyI CCWWGG 3 cut(s) 475, 880, 1062
TaaI ACNGT 1 cut(s) 189
TaiI ACGT 2 cut(s) 558, 786
TaqI TCGA 3 cut(s) 159, 390, 920
TaqII GACCGA 1 cut(s) 1034
TasI AATT 1 cut(s) 231
TatI WGTACW 2 cut(s) 1026, 1049
TauI GCSGC 1 cut(s) 797
TfiI GAWTC 4 cut(s) 86, 324, 342, 366
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 4 cut(s) 133, 229, 280, 343
TseFI GTSAC 2 cut(s) 121, 628
TseI GCWGC 4 cut(s) 716, 736, 839, 1000
Tsp45I GTSAC 2 cut(s) 121, 628
TspDTI ATGAA 2 cut(s) 778, 1055
TspGWI ACGGA 3 cut(s) 468, 688, 951
TspMI CCCGGG 1 cut(s) 132
TspRI CASTG 4 cut(s) 133, 229, 280, 343
Tth111I GACNNNGTC 1 cut(s) 554
VpaK11BI GGWCC 8 cut(s) 25, 425, 463, 641, 777, 884, 1017, 1077
XceI RCATGY 1 cut(s) 1033
XmaI CCCGGG 1 cut(s) 132
XmiI GTMKAC 1 cut(s) 561
XspI CTAG 5 cut(s) 525, 647, 740, 1074, 1081
ZrmI AGTACT 1 cut(s) 1051
Zsp2I ATGCAT 1 cut(s) 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.