Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0003153.1

Orthogroup ID

OG0013612

Physical Location & Seq

Genomic Coordinates Reverse (-)

84097 .. 86320

Gene Structure 2224 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0003153.1_g000023.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

atgatcttgcaggatgacattttcttaatttgctcgaatgcaatgcagtacaatgccccagatactatatattttcgacaggcgcgctccatccatgagcttgcgaaaaagaactttgaaaatttgaggcaagatagtgatgataatgaaccagaacccaaggttgtaagaaggggtagaccaccaaccaaaaatctgaagaaaccacttggcaggccttccttggagcgcgctggttcagagttctcagatgccactctcgctactggggcagaaaatgccatattgtccaattatgatcgttcacgaagtaatgatgtgtatactagttggttggctgataacaaatttgagaggaatgatgacttaacaggttccatgttgagaggtaattccaagactgggagaaagcagtttgtatttgatgagaacaagcgtaatacatatctacactctcatccctcggctaatggacgagagtcaactgtgttgactacatttgatcgagaaagaaaacagctaatgcctgtagggcttcagtcagatggttatgcaaggagcctggctcgatttgcagcgaatcttggacctgttacttggagaattgctgcacaaaggatccaaaggtctttgcctgctggtgttaagttcggccctgggtgggttggtgaaaatgatgttgctcctcataggccgctggtattagcctcatcctcaaaagagcagccatcttcaccacagcccttgcccattcgtgcaaactcgtcttctgctgcaatatcttgctctattgaaccaaataaagataaattatcagacaaaccttcaggtcataacttgtcagagaaacatgccccttctatttctgcagggccggatgaccatccaagcagtcctcccccactgtctgctgccacctcatcagatcccttaattgctaacagatcccctgaatccctcacaggaaaaggagaaactgttgaaggatcaaactctcgtggtgggattaatatgatgaacagtaatcatggtggaatcaggccaaagcccccttttcagatccatcggagtcctgaaatacaacctggggtgaatggattgaatgggacatatggattaaaccgtcttgctcaggtagggaaacaagttggcgtgaccaggcctggtggcttcagcttccaatcatctcaattgcttgaccaagtctccaggactaatactaacctgactcatccagcgacttcaaacagcttaaattcagaagaaaccaagccctctgaagatccaggtacaaagcagtcttctggttcatattcaaattcagtgaaaaatgagtcattgccagcccgaacatcggggcttcatccccggtcatcttggcgagggtcatcccaaccgattaaacctaatccaggattctctcctcaacagaagcctgactcagttccaccagatctgaacgtcagatttcagtcaccagggtcccctagttctagccgggctgattcaacgcagccggatttagcattgcagctctaa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000123 GO:0002576 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016192 GO:0016569 GO:0016570 GO:0016573 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019538 GO:0031248 GO:0031974 GO:0031981 GO:0032940 GO:0032991 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043543 GO:0043966 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045055 GO:0046903 GO:0051179 GO:0051234 GO:0051276 GO:0070013 GO:0070775 GO:0070776 GO:0071704 GO:0071840 GO:1901564 GO:1902493 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Bromodomain

Protein Analysis

512

Amino Acids

55.52

Weight (kDa)

9.53

Isoelectric Point (pI)

59.65

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013612)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g16690
malus_domestica	MD05G1135100.v1.1 MD10G1140600.v1.1
prunus_persica	Prupe.8G179000_v2.0.a1
pyrus_communis	pycom05g13170 pycom10g11920
rosa_chinensis	RchiOBHm_Chr6g0280831
rosa_laevigata	RLG00000013027
rosa_multiflora	Rmu_co8368811.1_g000001 Rmu_sc0003153.1_g000023 Rmu_sc0038972.1_g000001
rosa_roxburghii	Rroxscaffold_7G00187590
rosa_rugosa	Rorug06G0134800
rosa_samantha	Rh6AG248200 Rh6BG249700 Rh6CG250900 Rh6DG242500
rosa_wichuraiana	Rw6G021450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	178, 323
AccII	CGCG	2 cut(s)	85, 231
AciI	CCGC	1 cut(s)	695
AclWI	GGATC	7 cut(s)	613, 626, 920, 939, 994, 1054, 1277
AcsI	RAATTY	4 cut(s)	121, 347, 1255, 1318
AcuI	CTGAAG	5 cut(s)	218, 521, 810, 1156, 1299
AfaI	GTAC	2 cut(s)	50, 1291
AfiI	CCNNNNNNNGG	4 cut(s)	402, 661, 1354, 1412
AgsI	TTSAA	7 cut(s)	119, 794, 983, 1102, 1245, 1317, 1509
AhlI	ACTAGT	1 cut(s)	326
AjnI	CCWGG	9 cut(s)	561, 655, 1084, 1157, 1162, 1208, 1285, 1411, 1477
AluBI	AGCT	5 cut(s)	100, 520, 1176, 1251, 1534
AluI	AGCT	5 cut(s)	100, 520, 1176, 1251, 1534
Alw26I	GTCTC	1 cut(s)	1210
AlwI	GGATC	7 cut(s)	613, 626, 920, 939, 994, 1054, 1277
AoxI	GGCC	6 cut(s)	215, 652, 692, 872, 1040, 1160
ApeKI	GCWGC	7 cut(s)	575, 608, 724, 773, 911, 1513, 1531
ApoI	RAATTY	4 cut(s)	121, 347, 1255, 1318
AseI	ATTAAT	1 cut(s)	1008
AspLEI	GCGC	4 cut(s)	85, 87, 231, 233
AspS9I	GGNCC	4 cut(s)	587, 653, 872, 1482
AsuC2I	CCSGG	2 cut(s)	1369, 1499
AsuHPI	GGTGA	4 cut(s)	680, 726, 1102, 1467
AvaII	GGWCC	2 cut(s)	587, 1482
BamHI	GGATCC	1 cut(s)	618
BauI	CACGAG	1 cut(s)	996
BbsI	GAAGAC	2 cut(s)	759, 1293
BbvI	GCAGC	6 cut(s)	587, 595, 736, 760, 898, 1525
BccI	CCATC	5 cut(s)	98, 539, 736, 891, 1071
BcgI	CGANNNNNNTGC	2 cut(s)	25, 59
BciT130I	CCWGG	9 cut(s)	563, 657, 1086, 1159, 1164, 1210, 1287, 1413, 1479
BcnI	CCSGG	2 cut(s)	1369, 1499
BcoDI	GTCTC	1 cut(s)	1210
BcuI	ACTAGT	1 cut(s)	326
BfaI	CTAG	3 cut(s)	327, 1488, 1494
BfmI	CTRYAG	2 cut(s)	528, 867
BglI	GCCNNNNNGGC	1 cut(s)	532
BglII	AGATCT	1 cut(s)	1453
BisI	GCNGC	8 cut(s)	576, 609, 695, 725, 774, 912, 1514, 1532
BlsI	GCNGC	8 cut(s)	577, 610, 696, 726, 775, 913, 1515, 1533
Bme18I	GGWCC	2 cut(s)	587, 1482
BmgT120I	GGNCC	4 cut(s)	587, 653, 872, 1482
BmiI	GGNNCC	5 cut(s)	376, 560, 620, 1483, 1484
BmrI	ACTGGG	2 cut(s)	276, 411
BmsI	GCATC	1 cut(s)	241
BmuI	ACTGGG	2 cut(s)	276, 411
BoxI	GACNNNNGTC	1 cut(s)	478
BpiI	GAAGAC	2 cut(s)	759, 1293
BplI	GAGNNNNNCTC	2 cut(s)	550, 582
BpmI	CTGGAG	1 cut(s)	1192
Bpu10I	CCTNAGC	1 cut(s)	1131
BpuMI	CCSGG	2 cut(s)	1369, 1499
BsaBI	GATNNNNATC	1 cut(s)	882
BsaJI	CCNNGG	8 cut(s)	159, 222, 462, 655, 656, 1085, 1367, 1478
BsaXI	ACNNNNNCTCC	4 cut(s)	963, 993, 1190, 1220
Bsc4I	CCNNNNNNNGG	4 cut(s)	402, 661, 1354, 1412
Bse1I	ACTGG	2 cut(s)	271, 406
Bse3DI	GCAATG	3 cut(s)	48, 1337, 1526
Bse8I	GATNNNNATC	1 cut(s)	882
BseBI	CCWGG	9 cut(s)	563, 657, 1086, 1159, 1164, 1210, 1287, 1413, 1479
BseDI	CCNNGG	8 cut(s)	159, 222, 462, 655, 656, 1085, 1367, 1478
BseGI	GGATG	9 cut(s)	19, 90, 457, 710, 883, 883, 1231, 1363, 1388
BseJI	GATNNNNATC	1 cut(s)	882
BseLI	CCNNNNNNNGG	4 cut(s)	402, 661, 1354, 1412
BseMI	GCAATG	3 cut(s)	48, 1337, 1526
BseMII	CTCAG	3 cut(s)	261, 1145, 1455
BseNI	ACTGG	2 cut(s)	271, 406
BsePI	GCGCGC	2 cut(s)	83, 229
BseRI	GAGGAG	2 cut(s)	675, 1413
BseXI	GCAGC	6 cut(s)	587, 595, 736, 760, 898, 1525
BsgI	GTGCAG	1 cut(s)	594
Bsh1236I	CGCG	2 cut(s)	85, 231
BshFI	GGCC	6 cut(s)	217, 654, 694, 874, 1042, 1162
BsiSI	CCGG	4 cut(s)	875, 1369, 1498, 1517
BslFI	GGGAC	2 cut(s)	1120, 1468
BslI	CCNNNNNNNGG	4 cut(s)	402, 661, 1354, 1412
BsmAI	GTCTC	1 cut(s)	1210
BsmFI	GGGAC	2 cut(s)	1120, 1468
BsmI	GAATGC	1 cut(s)	43
BsnI	GGCC	6 cut(s)	217, 654, 694, 874, 1042, 1162
BspACI	CCGC	1 cut(s)	695
BspANI	GGCC	6 cut(s)	217, 654, 694, 874, 1042, 1162
BspCNI	CTCAG	3 cut(s)	260, 1144, 1454
BspFNI	CGCG	2 cut(s)	85, 231
BspLI	GGNNCC	5 cut(s)	376, 560, 620, 1483, 1484
BspMAI	CTGCAG	1 cut(s)	871
BspPI	GGATC	7 cut(s)	613, 626, 920, 939, 994, 1054, 1277
BsrDI	GCAATG	3 cut(s)	48, 1337, 1526
BsrI	ACTGG	2 cut(s)	271, 406
BssECI	CCNNGG	8 cut(s)	159, 222, 462, 655, 656, 1085, 1367, 1478
BssHII	GCGCGC	2 cut(s)	83, 229
BssNAI	GTATAC	1 cut(s)	324
BssSI	CACGAG	1 cut(s)	996
BssT1I	CCWWGG	2 cut(s)	159, 222
Bst1107I	GTATAC	1 cut(s)	324
Bst2BI	CACGAG	1 cut(s)	996
Bst2UI	CCWGG	9 cut(s)	563, 657, 1086, 1159, 1164, 1210, 1287, 1413, 1479
Bst4CI	ACNGT	5 cut(s)	487, 906, 979, 1022, 1124
BstAPI	GCANNNNNTGC	1 cut(s)	278
BstC8I	GCNNGC	6 cut(s)	85, 102, 215, 231, 636, 1344
BstDEI	CTNAG	3 cut(s)	247, 1131, 1441
BstENI	CCTNNNNNAGG	1 cut(s)	1410
BstF5I	GGATG	9 cut(s)	19, 90, 457, 710, 883, 883, 1231, 1363, 1388
BstFNI	CGCG	2 cut(s)	85, 231
BstHHI	GCGC	4 cut(s)	85, 87, 231, 233
BstMAI	GTCTC	1 cut(s)	1210
BstMWI	GCNNNNNNNGC	5 cut(s)	260, 269, 278, 532, 572
BstNI	CCWGG	9 cut(s)	563, 657, 1086, 1159, 1164, 1210, 1287, 1413, 1479
BstNSI	RCATGY	1 cut(s)	854
BstPAI	GACNNNNGTC	1 cut(s)	478
BstSFI	CTRYAG	2 cut(s)	528, 867
BstUI	CGCG	2 cut(s)	85, 231
BstV1I	GCAGC	6 cut(s)	587, 595, 736, 760, 898, 1525
BstV2I	GAAGAC	2 cut(s)	759, 1293
BstX2I	RGATCY	6 cut(s)	618, 925, 944, 1059, 1282, 1453
BstYI	RGATCY	6 cut(s)	618, 925, 944, 1059, 1282, 1453
BstZ17I	GTATAC	1 cut(s)	324
BsuRI	GGCC	6 cut(s)	217, 654, 694, 874, 1042, 1162
BtsCI	GGATG	9 cut(s)	19, 90, 457, 710, 883, 883, 1231, 1363, 1388
BtsIMutI	CAGTG	2 cut(s)	902, 1329
Cac8I	GCNNGC	6 cut(s)	85, 102, 215, 231, 636, 1344
CfoI	GCGC	4 cut(s)	85, 87, 231, 233
Cfr13I	GGNCC	4 cut(s)	587, 653, 872, 1482
Csp6I	GTAC	2 cut(s)	49, 1290
CspCI	CAANNNNNGTGG	2 cut(s)	726, 761
CviAII	CATG	4 cut(s)	95, 379, 851, 1028
CviQI	GTAC	2 cut(s)	49, 1290
DdeI	CTNAG	3 cut(s)	247, 1131, 1441
Eco130I	CCWWGG	2 cut(s)	159, 222
Eco147I	AGGCCT	2 cut(s)	217, 1162
Eco47I	GGWCC	2 cut(s)	587, 1482
Eco57I	CTGAAG	5 cut(s)	218, 521, 810, 1156, 1299
EcoNI	CCTNNNNNAGG	1 cut(s)	1410
EcoO109I	RGGNCCY	1 cut(s)	1482
EcoRII	CCWGG	9 cut(s)	561, 655, 1084, 1157, 1162, 1208, 1285, 1411, 1477
EcoT14I	CCWWGG	2 cut(s)	159, 222
ErhI	CCWWGG	2 cut(s)	159, 222
FaeI	CATG	4 cut(s)	98, 382, 854, 1031
FaqI	GGGAC	2 cut(s)	1120, 1468
FatI	CATG	4 cut(s)	94, 378, 850, 1027
FauNDI	CATATG	1 cut(s)	1111
FblI	GTMKAC	2 cut(s)	178, 323
Fnu4HI	GCNGC	8 cut(s)	576, 609, 695, 725, 774, 912, 1514, 1532
FokI	GGATG	9 cut(s)	26, 77, 444, 697, 870, 890, 1218, 1350, 1375
Fsp4HI	GCNGC	8 cut(s)	576, 609, 695, 725, 774, 912, 1514, 1532
FspBI	CTAG	3 cut(s)	327, 1488, 1494
GlaI	GCGC	4 cut(s)	84, 86, 230, 232
GluI	GCNGC	8 cut(s)	576, 609, 695, 725, 774, 912, 1514, 1532
GsuI	CTGGAG	1 cut(s)	1192
HaeIII	GGCC	6 cut(s)	217, 654, 694, 874, 1042, 1162
HapII	CCGG	4 cut(s)	875, 1369, 1498, 1517
HhaI	GCGC	4 cut(s)	85, 87, 231, 233
Hin1II	CATG	4 cut(s)	98, 382, 854, 1031
Hin6I	GCGC	4 cut(s)	83, 85, 229, 231
HinP1I	GCGC	4 cut(s)	83, 85, 229, 231
HincII	GTYRAC	2 cut(s)	483, 492
HindII	GTYRAC	2 cut(s)	483, 492
HpaII	CCGG	4 cut(s)	875, 1369, 1498, 1517
HphI	GGTGA	4 cut(s)	680, 726, 1102, 1467
Hpy166II	GTNNAC	5 cut(s)	179, 305, 324, 483, 492
Hpy188III	TCNNGA	3 cut(s)	306, 506, 1073
Hpy8I	GTNNAC	5 cut(s)	179, 305, 324, 483, 492
HpyAV	CCTTC	5 cut(s)	165, 228, 834, 867, 977
HpyCH4III	ACNGT	5 cut(s)	487, 906, 979, 1022, 1124
HpyCH4IV	ACGT	1 cut(s)	1461
HpyF10VI	GCNNNNNNNGC	5 cut(s)	260, 269, 278, 532, 572
HpyF3I	CTNAG	3 cut(s)	247, 1131, 1441
HpySE526I	ACGT	1 cut(s)	1461
Hsp92II	CATG	4 cut(s)	98, 382, 854, 1031
HspAI	GCGC	4 cut(s)	83, 85, 229, 231
KflI	GGGWCCC	1 cut(s)	1482
LmnI	GCTCC	4 cut(s)	92, 226, 558, 688
Lsp1109I	GCAGC	6 cut(s)	587, 595, 736, 760, 898, 1525
LweI	GCATC	1 cut(s)	241
MaeI	CTAG	3 cut(s)	327, 1488, 1494
MaeII	ACGT	1 cut(s)	1461
MaeIII	GTNAC	3 cut(s)	592, 1153, 1473
MboII	GAAGA	6 cut(s)	211, 723, 759, 1274, 1292, 1293
MfeI	CAATTG	1 cut(s)	1190
MflI	RGATCY	6 cut(s)	618, 925, 944, 1059, 1282, 1453
MlyI	GAGTC	5 cut(s)	488, 1078, 1222, 1343, 1433
MseI	TTAA	8 cut(s)	26, 368, 645, 932, 1008, 1118, 1253, 1401
MspA1I	CMGCKG	1 cut(s)	697
MspI	CCGG	4 cut(s)	875, 1369, 1498, 1517
MunI	CAATTG	1 cut(s)	1190
Mva1269I	GAATGC	1 cut(s)	43
MvaI	CCWGG	9 cut(s)	563, 657, 1086, 1159, 1164, 1210, 1287, 1413, 1479
MvnI	CGCG	2 cut(s)	85, 231
MwoI	GCNNNNNNNGC	5 cut(s)	260, 269, 278, 532, 572
NciI	CCSGG	2 cut(s)	1369, 1499
NdeI	CATATG	1 cut(s)	1111
NlaIII	CATG	4 cut(s)	98, 382, 854, 1031
NlaIV	GGNNCC	5 cut(s)	376, 560, 620, 1483, 1484
NmeAIII	GCCGAG	1 cut(s)	443
NmuCI	GTSAC	2 cut(s)	1153, 1473
NspI	RCATGY	1 cut(s)	854
PasI	CCCWGGG	1 cut(s)	656
PauI	GCGCGC	2 cut(s)	83, 229
PceI	AGGCCT	2 cut(s)	217, 1162
PctI	GAATGC	1 cut(s)	43
PfeI	GAWTC	5 cut(s)	580, 953, 1035, 1416, 1505
PflFI	GACNNNGTC	1 cut(s)	1202
PfoI	TCCNGGA	2 cut(s)	1208, 1411
PkrI	GCNGC	8 cut(s)	577, 610, 696, 726, 775, 913, 1515, 1533
PleI	GAGTC	5 cut(s)	487, 1077, 1222, 1342, 1433
PpsI	GAGTC	5 cut(s)	487, 1077, 1222, 1342, 1433
PpuMI	RGGWCCY	1 cut(s)	1482
PshAI	GACNNNNGTC	1 cut(s)	478
PshBI	ATTAAT	1 cut(s)	1008
Psp5II	RGGWCCY	1 cut(s)	1482
Psp6I	CCWGG	9 cut(s)	561, 655, 1084, 1157, 1162, 1208, 1285, 1411, 1477
PspGI	CCWGG	9 cut(s)	561, 655, 1084, 1157, 1162, 1208, 1285, 1411, 1477
PspN4I	GGNNCC	5 cut(s)	376, 560, 620, 1483, 1484
PspPI	GGNCC	4 cut(s)	587, 653, 872, 1482
PspPPI	RGGWCCY	1 cut(s)	1482
PstI	CTGCAG	1 cut(s)	871
PsuI	RGATCY	6 cut(s)	618, 925, 944, 1059, 1282, 1453
PsyI	GACNNNGTC	1 cut(s)	1202
PteI	GCGCGC	2 cut(s)	83, 229
RsaI	GTAC	2 cut(s)	50, 1291
RsaNI	GTAC	2 cut(s)	49, 1290
SaqAI	TTAA	8 cut(s)	26, 368, 645, 932, 1008, 1118, 1253, 1401
SatI	GCNGC	8 cut(s)	576, 609, 695, 725, 774, 912, 1514, 1532
Sau96I	GGNCC	4 cut(s)	587, 653, 872, 1482
SchI	GAGTC	5 cut(s)	488, 1078, 1222, 1343, 1433
SfaNI	GCATC	1 cut(s)	241
SfcI	CTRYAG	2 cut(s)	528, 867
SinI	GGWCC	2 cut(s)	587, 1482
SpeI	ACTAGT	1 cut(s)	326
SseBI	AGGCCT	2 cut(s)	217, 1162
SsiI	CCGC	1 cut(s)	695
SspMI	CTAG	3 cut(s)	327, 1488, 1494
StuI	AGGCCT	2 cut(s)	217, 1162
StyI	CCWWGG	2 cut(s)	159, 222
TaaI	ACNGT	5 cut(s)	487, 906, 979, 1022, 1124
TaiI	ACGT	1 cut(s)	1464
TaqI	TCGA	4 cut(s)	35, 76, 505, 568
TatI	WGTACW	1 cut(s)	48
TauI	GCSGC	1 cut(s)	697
TfiI	GAWTC	5 cut(s)	580, 953, 1035, 1416, 1505
Tru1I	TTAA	8 cut(s)	26, 368, 645, 932, 1008, 1118, 1253, 1401
Tru9I	TTAA	8 cut(s)	26, 368, 645, 932, 1008, 1118, 1253, 1401
TscAI	CASTG	2 cut(s)	909, 1329
TseFI	GTSAC	2 cut(s)	1153, 1473
TseI	GCWGC	7 cut(s)	575, 608, 724, 773, 911, 1513, 1531
Tsp45I	GTSAC	2 cut(s)	1153, 1473
TspDTI	ATGAA	4 cut(s)	162, 1031, 1299, 1352
TspRI	CASTG	2 cut(s)	909, 1329
Tth111I	GACNNNGTC	1 cut(s)	1202
VpaK11BI	GGWCC	2 cut(s)	587, 1482
VspI	ATTAAT	1 cut(s)	1008
XagI	CCTNNNNNAGG	1 cut(s)	1410
XapI	RAATTY	4 cut(s)	121, 347, 1255, 1318
XceI	RCATGY	1 cut(s)	854
XmiI	GTMKAC	2 cut(s)	178, 323
XspI	CTAG	3 cut(s)	327, 1488, 1494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.