Rmu_sc0003400.1_g000027

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003400.1
OG0001598
Physical Location & Seq
Forward (+)
79110 .. 86949
7840 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1350 bp
atggcatgcattatccttcataatatgattgttgaggatgaatgtgccgattactacaatggcgagtttgatgatgaaaaactggatccaaataggtccagaagagctcgagctcgcatctcaaacgaaaggctctcctttcaagattcacttgaccgtgtctggcgcatggactatggtgtctccgacagcactggtactgatgatgatcttcctccatctcctcaatacagaattccaagacggggtcaatttactggaattgaaagatctgcagtaggttctatctcgttcgctaggaagtatactgacatggaggcccaaattcatcacctagagcaagaagcatactgtgcagtcctgcgtgctttcaaagcccagtctgatgcgattacttgggagaaggaaggtttgattacagaacttaggaaagaactgagagtatccgatgatgaacacagagagcttcttggcagggttaactctgatgatatcatcagtaagataagagagtggagaacggcaggtgggcatcaagctgcgagattcagtgcatcccagcctgtttatgaccttgtatccagtcctactgtttcagcatcccgaaagaaacagaaaacatcccaatcgcaccagggtcagccattccctggtttatcctcaatgaagtctatgcagtacccttctacaggaccttctttgagtaggcagctcactagccgcagtactactggtccccttgccataactgaacctgccgaagcagcaacatatgatttaattggaaagaaagtgtggatgagatggcctgcagacaacagcttctatgaagcccttataacagactacaaccctaatgatggtcgacatgctttgatctacgataaaaatacagcaaatgagacatttgaatgggtagatttcaaagagatatctcgtgaggatatccgttgggaaggtgaggaactggggctatctcatcaagggaaccatggcagtggacaaggacgtagggttaagaaatctacgaaccatggtggtgttggtccagtttctgggagaggtcgaggacatataaagcctcaatcaaagaaacaacttcttccttcacaaaacggcataggaaataagttgccagatgatctagaattattgaatactgattctctggtaaaggaggtggagagagttttcaatgcaagtaatccggacccctttgagcttgaaaaggcaaggaaaatgctgaaagatcacgaacaggcacttgttgatgccattgaaaggcttgcatatgcatccgatggtgaaagtgatggagagcatggagagcatgcatttatgcatggttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

449

Amino Acids

50.55

Weight (kDa)

5.81

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 107 - 175 5.8e-31 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 839
AarI CACCTGC 1 cut(s) 515
AasI GACNNNNNNGTC 1 cut(s) 179
Acc36I ACCTGC 2 cut(s) 515, 763
AccB7I CCANNNNNTGG 2 cut(s) 650, 1055
AccI GTMKAC 2 cut(s) 305, 865
AccIII TCCGGA 1 cut(s) 1207
AciI CCGC 1 cut(s) 721
AclWI GGATC 2 cut(s) 80, 93
AcsI RAATTY 2 cut(s) 234, 324
AfaI GTAC 3 cut(s) 199, 680, 727
AfiI CCNNNNNNNGG 6 cut(s) 245, 650, 689, 860, 1055, 1281
AgsI TTSAA 9 cut(s) 143, 266, 373, 911, 925, 1156, 1195, 1226, 1280
AjnI CCWGG 2 cut(s) 633, 649
AluBI AGCT 7 cut(s) 107, 113, 466, 539, 712, 822, 1222
AluI AGCT 7 cut(s) 107, 113, 466, 539, 712, 822, 1222
Alw21I GWGCWC 2 cut(s) 109, 115
Alw26I GTCTC 2 cut(s) 187, 896
AlwI GGATC 2 cut(s) 80, 93
AlwNI CAGNNNCTG 1 cut(s) 1055
Ama87I CYCGRG 1 cut(s) 108
Aor13HI TCCGGA 1 cut(s) 1207
AoxI GGCC 2 cut(s) 318, 806
ApeKI GCWGC 3 cut(s) 539, 709, 764
ApoI RAATTY 2 cut(s) 234, 324
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 6 cut(s) 96, 319, 692, 734, 1046, 1210
AsuHPI GGTGA 3 cut(s) 323, 971, 1316
AvaI CYCGRG 1 cut(s) 108
AvaII GGWCC 5 cut(s) 96, 692, 734, 1046, 1210
BamHI GGATCC 1 cut(s) 85
BanII GRGCYC 2 cut(s) 109, 115
BauI CACGAG 1 cut(s) 936
Bbv12I GWGCWC 2 cut(s) 109, 115
BbvI GCAGC 3 cut(s) 526, 721, 776
BccI CCATC 5 cut(s) 226, 798, 854, 1295, 1307
BceAI ACGGC 2 cut(s) 537, 1132
BciT130I CCWGG 2 cut(s) 635, 651
BciVI GTATCC 2 cut(s) 454, 589
BcoDI GTCTC 2 cut(s) 187, 896
BfaI CTAG 4 cut(s) 297, 335, 717, 1145
BfmI CTRYAG 3 cut(s) 273, 687, 810
BfuAI ACCTGC 2 cut(s) 515, 763
BfuI GTATCC 2 cut(s) 454, 589
BglII AGATCT 1 cut(s) 269
BisI GCNGC 4 cut(s) 540, 710, 721, 765
BlsI GCNGC 4 cut(s) 541, 711, 722, 766
BmcAI AGTACT 1 cut(s) 727
Bme1390I CCNGG 2 cut(s) 635, 651
Bme18I GGWCC 5 cut(s) 96, 692, 734, 1046, 1210
BmeT110I CYCGRG 1 cut(s) 108
BmgT120I GGNCC 6 cut(s) 96, 319, 692, 734, 1046, 1210
BmiI GGNNCC 4 cut(s) 87, 736, 989, 1212
BmrFI CCNGG 2 cut(s) 635, 651
BmrI ACTGGG 2 cut(s) 373, 977
BmsI GCATC 7 cut(s) 126, 376, 541, 563, 608, 1261, 1304
BmuI ACTGGG 2 cut(s) 373, 977
BsaBI GATNNNNATC 1 cut(s) 207
BsaJI CCNNGG 4 cut(s) 634, 649, 991, 1033
BsaWI WCCGGW 1 cut(s) 1207
Bsc4I CCNNNNNNNGG 6 cut(s) 245, 650, 689, 860, 1055, 1281
Bse1I ACTGG 8 cut(s) 87, 199, 262, 379, 582, 736, 972, 1049
Bse8I GATNNNNATC 1 cut(s) 207
BseAI TCCGGA 1 cut(s) 1207
BseBI CCWGG 2 cut(s) 635, 651
BseDI CCNNGG 4 cut(s) 634, 649, 991, 1033
BseGI GGATG 6 cut(s) 43, 554, 599, 620, 804, 1295
BseJI GATNNNNATC 1 cut(s) 207
BseLI CCNNNNNNNGG 6 cut(s) 245, 650, 689, 860, 1055, 1281
BseMII CTCAG 1 cut(s) 428
BseNI ACTGG 8 cut(s) 87, 199, 262, 379, 582, 736, 972, 1049
BseRI GAGGAG 1 cut(s) 213
BseXI GCAGC 3 cut(s) 526, 721, 776
BseYI CCCAGC 1 cut(s) 558
BsgI GTGCAG 1 cut(s) 375
BshFI GGCC 2 cut(s) 320, 808
BsiHKAI GWGCWC 2 cut(s) 109, 115
BsiHKCI CYCGRG 1 cut(s) 108
BsiSI CCGG 1 cut(s) 1208
BslFI GGGAC 1 cut(s) 720
BslI CCNNNNNNNGG 6 cut(s) 245, 650, 689, 860, 1055, 1281
BsmAI GTCTC 2 cut(s) 187, 896
BsmFI GGGAC 1 cut(s) 720
BsnI GGCC 2 cut(s) 320, 808
BsoBI CYCGRG 1 cut(s) 108
Bsp1286I GDGCHC 2 cut(s) 109, 115
Bsp13I TCCGGA 1 cut(s) 1207
Bsp143I GATC 6 cut(s) 85, 208, 269, 876, 1141, 1249
Bsp19I CCATGG 2 cut(s) 991, 1033
BspACI CCGC 1 cut(s) 721
BspANI GGCC 2 cut(s) 320, 808
BspCNI CTCAG 1 cut(s) 429
BspEI TCCGGA 1 cut(s) 1207
BspLI GGNNCC 4 cut(s) 87, 736, 989, 1212
BspMAI CTGCAG 2 cut(s) 277, 814
BspMI ACCTGC 2 cut(s) 515, 763
BspPI GGATC 2 cut(s) 80, 93
BspQI GCTCTTC 1 cut(s) 97
BsrI ACTGG 8 cut(s) 87, 199, 262, 379, 582, 736, 972, 1049
BssECI CCNNGG 4 cut(s) 634, 649, 991, 1033
BssMI GATC 6 cut(s) 85, 208, 269, 876, 1141, 1249
BssNAI GTATAC 1 cut(s) 306
BssSI CACGAG 1 cut(s) 936
BssT1I CCWWGG 2 cut(s) 991, 1033
Bst1107I GTATAC 1 cut(s) 306
Bst2BI CACGAG 1 cut(s) 936
Bst2UI CCWGG 2 cut(s) 635, 651
Bst4CI ACNGT 3 cut(s) 158, 353, 592
Bst6I CTCTTC 1 cut(s) 97
BstAPI GCANNNNNTGC 1 cut(s) 353
BstC8I GCNNGC 6 cut(s) 7, 115, 366, 810, 1287, 1332
BstDEI CTNAG 2 cut(s) 425, 437
BstDSI CCRYGG 2 cut(s) 991, 1033
BstENI CCTNNNNNAGG 1 cut(s) 687
BstF5I GGATG 6 cut(s) 43, 554, 599, 620, 804, 1295
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 6 cut(s) 88, 211, 272, 879, 1144, 1252
BstMAI GTCTC 2 cut(s) 187, 896
BstMBI GATC 6 cut(s) 85, 208, 269, 876, 1141, 1249
BstMWI GCNNNNNNNGC 4 cut(s) 353, 374, 764, 1327
BstNI CCWGG 2 cut(s) 635, 651
BstNSI RCATGY 3 cut(s) 9, 872, 1334
BstSCI CCNGG 2 cut(s) 633, 649
BstSFI CTRYAG 3 cut(s) 273, 687, 810
BstV1I GCAGC 3 cut(s) 526, 721, 776
BstX2I RGATCY 2 cut(s) 85, 269
BstXI CCANNNNNNTGG 1 cut(s) 998
BstYI RGATCY 2 cut(s) 85, 269
BstZ17I GTATAC 1 cut(s) 306
BsuI GTATCC 2 cut(s) 454, 589
BsuRI GGCC 2 cut(s) 320, 808
BtgI CCRYGG 2 cut(s) 991, 1033
BtsCI GGATG 6 cut(s) 43, 554, 599, 620, 804, 1295
BtsI GCAGTG 1 cut(s) 1003
BtsIMutI CAGTG 3 cut(s) 192, 556, 1003
BveI ACCTGC 2 cut(s) 515, 763
Cac8I GCNNGC 6 cut(s) 7, 115, 366, 810, 1287, 1332
CaiI CAGNNNCTG 1 cut(s) 1055
CfoI GCGC 1 cut(s) 168
Cfr13I GGNCC 6 cut(s) 96, 319, 692, 734, 1046, 1210
Csp6I GTAC 3 cut(s) 198, 679, 726
CviAII CATG 9 cut(s) 6, 169, 313, 869, 992, 1034, 1322, 1331, 1343
CviQI GTAC 3 cut(s) 198, 679, 726
DdeI CTNAG 2 cut(s) 425, 437
DpnI GATC 6 cut(s) 87, 210, 271, 878, 1143, 1251
DpnII GATC 6 cut(s) 85, 208, 269, 876, 1141, 1249
DrdI GACNNNNNNGTC 1 cut(s) 179
DseDI GACNNNNNNGTC 1 cut(s) 179
Eam1104I CTCTTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 97
Ecl136II GAGCTC 2 cut(s) 107, 113
Eco130I CCWWGG 2 cut(s) 991, 1033
Eco24I GRGCYC 2 cut(s) 109, 115
Eco32I GATATC 3 cut(s) 493, 933, 946
Eco47I GGWCC 5 cut(s) 96, 692, 734, 1046, 1210
Eco53kI GAGCTC 2 cut(s) 107, 113
Eco88I CYCGRG 1 cut(s) 108
EcoICRI GAGCTC 2 cut(s) 107, 113
EcoNI CCTNNNNNAGG 1 cut(s) 687
EcoO109I RGGNCCY 1 cut(s) 692
EcoRI GAATTC 1 cut(s) 234
EcoRII CCWGG 2 cut(s) 633, 649
EcoRV GATATC 3 cut(s) 493, 933, 946
EcoT14I CCWWGG 2 cut(s) 991, 1033
EcoT22I ATGCAT 4 cut(s) 11, 1297, 1336, 1344
EcoT38I GRGCYC 2 cut(s) 109, 115
ErhI CCWWGG 2 cut(s) 991, 1033
FaeI CATG 9 cut(s) 9, 172, 316, 872, 995, 1037, 1325, 1334, 1346
FalI AAGNNNNNCTT 2 cut(s) 135, 167
FaqI GGGAC 1 cut(s) 720
FatI CATG 9 cut(s) 5, 168, 312, 868, 991, 1033, 1321, 1330, 1342
FauNDI CATATG 2 cut(s) 772, 1291
FblI GTMKAC 2 cut(s) 305, 865
Fnu4HI GCNGC 4 cut(s) 540, 710, 721, 765
FokI GGATG 6 cut(s) 50, 541, 586, 607, 811, 1282
FriOI GRGCYC 2 cut(s) 109, 115
Fsp4HI GCNGC 4 cut(s) 540, 710, 721, 765
FspBI CTAG 4 cut(s) 297, 335, 717, 1145
GlaI GCGC 1 cut(s) 167
GluI GCNGC 4 cut(s) 540, 710, 721, 765
GsaI CCCAGC 1 cut(s) 562
HaeIII GGCC 2 cut(s) 320, 808
HapII CCGG 1 cut(s) 1208
HhaI GCGC 1 cut(s) 168
Hin1II CATG 9 cut(s) 9, 172, 316, 872, 995, 1037, 1325, 1334, 1346
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HincII GTYRAC 2 cut(s) 481, 866
HindII GTYRAC 2 cut(s) 481, 866
HinfI GANTC 3 cut(s) 146, 546, 1163
HpaI GTTAAC 1 cut(s) 481
HpaII CCGG 1 cut(s) 1208
HphI GGTGA 3 cut(s) 323, 971, 1316
Hpy166II GTNNAC 4 cut(s) 306, 481, 866, 1001
Hpy188I TCNGA 5 cut(s) 187, 385, 448, 487, 1300
Hpy188III TCNNGA 7 cut(s) 99, 143, 603, 938, 1145, 1208, 1253
Hpy8I GTNNAC 4 cut(s) 306, 481, 866, 1001
HpyAV CCTTC 7 cut(s) 26, 397, 401, 693, 705, 950, 1116
HpyCH4III ACNGT 3 cut(s) 158, 353, 592
HpyCH4IV ACGT 1 cut(s) 1009
HpyF10VI GCNNNNNNNGC 4 cut(s) 353, 374, 764, 1327
HpyF3I CTNAG 2 cut(s) 425, 437
HpySE526I ACGT 1 cut(s) 1009
Hsp92II CATG 9 cut(s) 9, 172, 316, 872, 995, 1037, 1325, 1334, 1346
HspAI GCGC 1 cut(s) 166
Kpn2I TCCGGA 1 cut(s) 1207
KspAI GTTAAC 1 cut(s) 481
Kzo9I GATC 6 cut(s) 85, 208, 269, 876, 1141, 1249
LguI GCTCTTC 1 cut(s) 97
Lsp1109I GCAGC 3 cut(s) 526, 721, 776
LweI GCATC 7 cut(s) 126, 376, 541, 563, 608, 1261, 1304
MaeI CTAG 4 cut(s) 297, 335, 717, 1145
MaeII ACGT 1 cut(s) 1009
MalI GATC 6 cut(s) 87, 210, 271, 878, 1143, 1251
MboI GATC 6 cut(s) 85, 208, 269, 876, 1141, 1249
MboII GAAGA 3 cut(s) 114, 203, 1094
MflI RGATCY 2 cut(s) 85, 269
MhlI GDGCHC 2 cut(s) 109, 115
MluCI AATT 6 cut(s) 234, 251, 261, 324, 780, 1148
MmeI TCCRAC 1 cut(s) 210
Mph1103I ATGCAT 4 cut(s) 11, 1297, 1336, 1344
MroI TCCGGA 1 cut(s) 1207
MseI TTAA 3 cut(s) 480, 779, 1017
MslI CAYNNNNRTG 3 cut(s) 910, 996, 1038
MspI CCGG 1 cut(s) 1208
MspR9I CCNGG 2 cut(s) 635, 651
MvaI CCWGG 2 cut(s) 635, 651
MwoI GCNNNNNNNGC 4 cut(s) 353, 374, 764, 1327
NcoI CCATGG 2 cut(s) 991, 1033
NdeI CATATG 2 cut(s) 772, 1291
NdeII GATC 6 cut(s) 85, 208, 269, 876, 1141, 1249
NlaIII CATG 9 cut(s) 9, 172, 316, 872, 995, 1037, 1325, 1334, 1346
NlaIV GGNNCC 4 cut(s) 87, 736, 989, 1212
NsiI ATGCAT 4 cut(s) 11, 1297, 1336, 1344
NspI RCATGY 3 cut(s) 9, 872, 1334
PaeI GCATGC 2 cut(s) 9, 1334
PaeR7I CTCGAG 1 cut(s) 108
PaqCI CACCTGC 1 cut(s) 515
PciSI GCTCTTC 1 cut(s) 97
PfeI GAWTC 3 cut(s) 146, 546, 1163
PflFI GACNNNGTC 2 cut(s) 158, 246
PflMI CCANNNNNTGG 2 cut(s) 650, 1055
PkrI GCNGC 4 cut(s) 541, 711, 722, 766
PpuMI RGGWCCY 1 cut(s) 692
PsiI TTATAA 1 cut(s) 839
Psp124BI GAGCTC 2 cut(s) 109, 115
Psp5II RGGWCCY 1 cut(s) 692
Psp6I CCWGG 2 cut(s) 633, 649
PspFI CCCAGC 1 cut(s) 558
PspGI CCWGG 2 cut(s) 633, 649
PspN4I GGNNCC 4 cut(s) 87, 736, 989, 1212
PspPI GGNCC 6 cut(s) 96, 319, 692, 734, 1046, 1210
PspPPI RGGWCCY 1 cut(s) 692
PspXI VCTCGAGB 1 cut(s) 108
PstI CTGCAG 2 cut(s) 277, 814
PstNI CAGNNNCTG 1 cut(s) 1055
PsuI RGATCY 2 cut(s) 85, 269
PsyI GACNNNGTC 2 cut(s) 158, 246
RsaI GTAC 3 cut(s) 199, 680, 727
RsaNI GTAC 3 cut(s) 198, 679, 726
RseI CAYNNNNRTG 3 cut(s) 910, 996, 1038
SacI GAGCTC 2 cut(s) 109, 115
SalI GTCGAC 1 cut(s) 864
SapI GCTCTTC 1 cut(s) 97
SaqAI TTAA 3 cut(s) 480, 779, 1017
SatI GCNGC 4 cut(s) 540, 710, 721, 765
Sau3AI GATC 6 cut(s) 85, 208, 269, 876, 1141, 1249
Sau96I GGNCC 6 cut(s) 96, 319, 692, 734, 1046, 1210
ScaI AGTACT 1 cut(s) 727
ScrFI CCNGG 2 cut(s) 635, 651
SduI GDGCHC 2 cut(s) 109, 115
SfaNI GCATC 7 cut(s) 126, 376, 541, 563, 608, 1261, 1304
SfcI CTRYAG 3 cut(s) 273, 687, 810
Sfr274I CTCGAG 1 cut(s) 108
SinI GGWCC 5 cut(s) 96, 692, 734, 1046, 1210
SlaI CTCGAG 1 cut(s) 108
SmiMI CAYNNNNRTG 3 cut(s) 910, 996, 1038
SmlI CTYRAG 1 cut(s) 108
SmoI CTYRAG 1 cut(s) 108
SphI GCATGC 2 cut(s) 9, 1334
Sse9I AATT 6 cut(s) 234, 251, 261, 324, 780, 1148
SsiI CCGC 1 cut(s) 721
SspMI CTAG 4 cut(s) 297, 335, 717, 1145
SstI GAGCTC 2 cut(s) 109, 115
StyD4I CCNGG 2 cut(s) 633, 649
StyI CCWWGG 2 cut(s) 991, 1033
TaaI ACNGT 3 cut(s) 158, 353, 592
TaiI ACGT 1 cut(s) 1012
TaqI TCGA 3 cut(s) 109, 865, 1066
TasI AATT 6 cut(s) 234, 251, 261, 324, 780, 1148
TatI WGTACW 1 cut(s) 725
TauI GCSGC 1 cut(s) 723
TfiI GAWTC 3 cut(s) 146, 546, 1163
Tru1I TTAA 3 cut(s) 480, 779, 1017
Tru9I TTAA 3 cut(s) 480, 779, 1017
TscAI CASTG 3 cut(s) 199, 556, 1003
TseI GCWGC 3 cut(s) 539, 709, 764
TspDTI ATGAA 7 cut(s) 8, 54, 90, 317, 468, 680, 843
TspGWI ACGGA 1 cut(s) 938
TspRI CASTG 3 cut(s) 199, 556, 1003
Tth111I GACNNNGTC 2 cut(s) 158, 246
Van91I CCANNNNNTGG 2 cut(s) 650, 1055
VpaK11BI GGWCC 5 cut(s) 96, 692, 734, 1046, 1210
XagI CCTNNNNNAGG 1 cut(s) 687
XapI RAATTY 2 cut(s) 234, 324
XbaI TCTAGA 1 cut(s) 1144
XceI RCATGY 3 cut(s) 9, 872, 1334
XcmI CCANNNNNNNNNTGG 1 cut(s) 1040
XhoI CTCGAG 1 cut(s) 108
XmiI GTMKAC 2 cut(s) 305, 865
XspI CTAG 4 cut(s) 297, 335, 717, 1145
ZrmI AGTACT 1 cut(s) 727
Zsp2I ATGCAT 4 cut(s) 11, 1297, 1336, 1344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.