Rmu_sc0003468.1_g000002

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003468.1
OG0010034
Physical Location & Seq
Reverse (-)
4516 .. 5184
669 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 669 bp
atgggaaattgttcttctctcaaaggaacctgtggggagtgtcgtaactctatcagggtccttacagattctgggggtatcctacagtttaaaggtcctaagttagcttcggaaatcctcgctggttttccaggctatggcatttttcagcagggcaaggcctcggtgccattgcctgaggaagaggccttaactagtggccagttctaccttcttccactgaggactaaagaggaagtgcctgctgattatggggtctctgtagaagttcaagaaatgggtgctgatgaagatttggatggtgcagaagaagctgagccggtgaagaagtcgtttgggggtgcatcggattgcgtggagaatttggcaaatgggtcaggaggaggtctggaagtaatgccgtgtggaggagatggggtttggagagtgaagcttgtgattgacactaagcagttagaggagatattgtctgaaggcaacactctggctctgatagagatgatgaggatggctgcaacagcaagtgctacaccgaagctgcagagtaagagcctatggggttcaggaggaggtgggggttggaattggaagcctatcttctccaacatgtttaaggcccccacggccataactgcccctatggccgtaactgatcaaagtgggatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

222

Amino Acids

23.27

Weight (kDa)

4.6

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 181 4.7e-13 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 166
AccB7I CCANNNNNTGG 1 cut(s) 137
AcoI YGGCCR 3 cut(s) 199, 624, 642
AcsI RAATTY 1 cut(s) 361
AcuI CTGAAG 1 cut(s) 492
AfiI CCNNNNNNNGG 2 cut(s) 137, 407
AflIII ACRYGT 1 cut(s) 606
AgsI TTSAA 1 cut(s) 272
AhlI ACTAGT 1 cut(s) 194
AjnI CCWGG 1 cut(s) 130
AluBI AGCT 4 cut(s) 107, 314, 433, 538
AluI AGCT 4 cut(s) 107, 314, 433, 538
Alw26I GTCTC 1 cut(s) 262
AlwNI CAGNNNCTG 1 cut(s) 71
AoxI GGCC 6 cut(s) 159, 186, 199, 615, 624, 642
ApeKI GCWGC 2 cut(s) 512, 538
ApoI RAATTY 1 cut(s) 361
Asp700I GAANNNNTTC 1 cut(s) 10
AspS9I GGNCC 3 cut(s) 58, 95, 616
AsuHPI GGTGA 1 cut(s) 334
AvaII GGWCC 2 cut(s) 58, 95
AxyI CCTNAGG 1 cut(s) 177
BalI TGGCCA 1 cut(s) 201
BanI GGYRCC 1 cut(s) 166
BbvI GCAGC 2 cut(s) 499, 525
BccI CCATC 3 cut(s) 293, 407, 502
BceAI ACGGC 3 cut(s) 385, 629, 639
BciT130I CCWGG 1 cut(s) 132
BciVI GTATCC 1 cut(s) 89
BclI TGATCA 1 cut(s) 652
BcoDI GTCTC 1 cut(s) 262
BcuI ACTAGT 1 cut(s) 194
BfaI CTAG 1 cut(s) 195
BfmI CTRYAG 3 cut(s) 83, 261, 539
BfuI GTATCC 1 cut(s) 89
BglI GCCNNNNNGGC 2 cut(s) 623, 641
BisI GCNGC 2 cut(s) 513, 539
BlpI GCTNAGC 1 cut(s) 315
BlsI GCNGC 2 cut(s) 514, 540
Bme1390I CCNGG 1 cut(s) 132
Bme18I GGWCC 2 cut(s) 58, 95
BmgT120I GGNCC 3 cut(s) 58, 95, 616
BmiI GGNNCC 4 cut(s) 28, 59, 168, 618
BmrFI CCNGG 1 cut(s) 132
BmsI GCATC 1 cut(s) 353
Bpu1102I GCTNAGC 1 cut(s) 315
BsaI GGTCTC 1 cut(s) 262
BsaJI CCNNGG 2 cut(s) 162, 621
BsaXI ACNNNNNCTCC 4 cut(s) 402, 432, 452, 482
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 407
Bse118I RCCGGY 1 cut(s) 319
Bse1I ACTGG 1 cut(s) 202
Bse21I CCTNAGG 1 cut(s) 177
Bse3DI GCAATG 1 cut(s) 170
BseBI CCWGG 1 cut(s) 132
BseDI CCNNGG 2 cut(s) 162, 621
BseGI GGATG 3 cut(s) 304, 513, 669
BseLI CCNNNNNNNGG 2 cut(s) 137, 407
BseMI GCAATG 1 cut(s) 170
BseMII CTCAG 3 cut(s) 168, 212, 306
BseNI ACTGG 1 cut(s) 202
BseRI GAGGAG 4 cut(s) 396, 423, 473, 582
BseXI GCAGC 2 cut(s) 499, 525
BsgI GTGCAG 1 cut(s) 324
BshFI GGCC 6 cut(s) 161, 188, 201, 617, 626, 644
BshNI GGYRCC 1 cut(s) 166
BsiSI CCGG 1 cut(s) 320
BslI CCNNNNNNNGG 2 cut(s) 137, 407
BsmAI GTCTC 1 cut(s) 262
BsnI GGCC 6 cut(s) 161, 188, 201, 617, 626, 644
Bso31I GGTCTC 1 cut(s) 262
Bsp143I GATC 1 cut(s) 652
Bsp1720I GCTNAGC 1 cut(s) 315
BspANI GGCC 6 cut(s) 161, 188, 201, 617, 626, 644
BspCNI CTCAG 3 cut(s) 169, 213, 307
BspLI GGNNCC 4 cut(s) 28, 59, 168, 618
BspMAI CTGCAG 1 cut(s) 543
BspT107I GGYRCC 1 cut(s) 166
BspTNI GGTCTC 1 cut(s) 262
BsrDI GCAATG 1 cut(s) 170
BsrFI RCCGGY 1 cut(s) 319
BsrI ACTGG 1 cut(s) 202
BssAI RCCGGY 1 cut(s) 319
BssECI CCNNGG 2 cut(s) 162, 621
BssMI GATC 1 cut(s) 652
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 1 cut(s) 87
Bst6I CTCTTC 1 cut(s) 177
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 5 cut(s) 99, 177, 221, 315, 447
BstDSI CCRYGG 1 cut(s) 621
BstF5I GGATG 3 cut(s) 304, 513, 669
BstKTI GATC 1 cut(s) 655
BstMAI GTCTC 1 cut(s) 262
BstMBI GATC 1 cut(s) 652
BstMWI GCNNNNNNNGC 5 cut(s) 311, 518, 623, 632, 641
BstNI CCWGG 1 cut(s) 132
BstNSI RCATGY 1 cut(s) 610
BstSCI CCNGG 1 cut(s) 130
BstSFI CTRYAG 3 cut(s) 83, 261, 539
BstV1I GCAGC 2 cut(s) 499, 525
Bsu36I CCTNAGG 1 cut(s) 177
BsuI GTATCC 1 cut(s) 89
BsuRI GGCC 6 cut(s) 161, 188, 201, 617, 626, 644
BtgI CCRYGG 1 cut(s) 621
BtsCI GGATG 3 cut(s) 304, 513, 669
BtsIMutI CAGTG 1 cut(s) 218
Cac8I GCNNGC 1 cut(s) 243
CaiI CAGNNNCTG 1 cut(s) 71
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 3 cut(s) 58, 95, 616
CviAII CATG 1 cut(s) 607
DdeI CTNAG 5 cut(s) 99, 177, 221, 315, 447
DpnI GATC 1 cut(s) 654
DpnII GATC 1 cut(s) 652
DraI TTTAAA 1 cut(s) 91
EaeI YGGCCR 3 cut(s) 199, 624, 642
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco147I AGGCCT 2 cut(s) 161, 188
Eco31I GGTCTC 1 cut(s) 262
Eco47I GGWCC 2 cut(s) 58, 95
Eco57I CTGAAG 1 cut(s) 492
Eco81I CCTNAGG 1 cut(s) 177
EcoO109I RGGNCCY 3 cut(s) 58, 95, 616
EcoRII CCWGG 1 cut(s) 130
FaeI CATG 1 cut(s) 610
FaiI YATR 6 cut(s) 138, 252, 556, 608, 629, 641
FalI AAGNNNNNCTT 2 cut(s) 581, 613
FatI CATG 1 cut(s) 606
FbaI TGATCA 1 cut(s) 652
Fnu4HI GCNGC 2 cut(s) 513, 539
FokI GGATG 2 cut(s) 311, 520
Fsp4HI GCNGC 2 cut(s) 513, 539
FspBI CTAG 1 cut(s) 195
GluI GCNGC 2 cut(s) 513, 539
HaeIII GGCC 6 cut(s) 161, 188, 201, 617, 626, 644
HapII CCGG 1 cut(s) 320
Hin1II CATG 1 cut(s) 610
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 1 cut(s) 68
HpaII CCGG 1 cut(s) 320
HphI GGTGA 1 cut(s) 334
Hpy188I TCNGA 4 cut(s) 112, 349, 472, 492
Hpy188III TCNNGA 4 cut(s) 272, 378, 389, 564
HpyAV CCTTC 2 cut(s) 221, 467
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4V TGCA 4 cut(s) 305, 344, 515, 541
HpyF10VI GCNNNNNNNGC 5 cut(s) 311, 518, 623, 632, 641
HpyF3I CTNAG 5 cut(s) 99, 177, 221, 315, 447
Hsp92II CATG 1 cut(s) 610
Ksp22I TGATCA 1 cut(s) 652
Kzo9I GATC 1 cut(s) 652
Lsp1109I GCAGC 2 cut(s) 499, 525
LweI GCATC 1 cut(s) 353
MaeI CTAG 1 cut(s) 195
MaeIII GTNAC 2 cut(s) 44, 646
MalI GATC 1 cut(s) 654
MboI GATC 1 cut(s) 652
MboII GAAGA 7 cut(s) 6, 194, 206, 302, 320, 337, 589
MlsI TGGCCA 1 cut(s) 201
MluCI AATT 3 cut(s) 7, 361, 583
MluNI TGGCCA 1 cut(s) 201
MmeI TCCRAC 2 cut(s) 560, 627
Mox20I TGGCCA 1 cut(s) 201
MroXI GAANNNNTTC 1 cut(s) 10
MscI TGGCCA 1 cut(s) 201
MseI TTAA 3 cut(s) 90, 191, 612
Msp20I TGGCCA 1 cut(s) 201
MspI CCGG 1 cut(s) 320
MspR9I CCNGG 1 cut(s) 132
MvaI CCWGG 1 cut(s) 132
MwoI GCNNNNNNNGC 5 cut(s) 311, 518, 623, 632, 641
NdeII GATC 1 cut(s) 652
NlaIII CATG 1 cut(s) 610
NlaIV GGNNCC 4 cut(s) 28, 59, 168, 618
NspI RCATGY 1 cut(s) 610
PceI AGGCCT 2 cut(s) 161, 188
PciI ACATGT 1 cut(s) 606
PdmI GAANNNNTTC 1 cut(s) 10
PfeI GAWTC 1 cut(s) 68
PflMI CCANNNNNTGG 1 cut(s) 137
PkrI GCNGC 2 cut(s) 514, 540
PpuMI RGGWCCY 2 cut(s) 58, 95
PscI ACATGT 1 cut(s) 606
Psp5II RGGWCCY 2 cut(s) 58, 95
Psp6I CCWGG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 4 cut(s) 28, 59, 168, 618
PspPI GGNCC 3 cut(s) 58, 95, 616
PspPPI RGGWCCY 2 cut(s) 58, 95
PstI CTGCAG 1 cut(s) 543
PstNI CAGNNNCTG 1 cut(s) 71
SaqAI TTAA 3 cut(s) 90, 191, 612
SatI GCNGC 2 cut(s) 513, 539
Sau3AI GATC 1 cut(s) 652
Sau96I GGNCC 3 cut(s) 58, 95, 616
ScrFI CCNGG 1 cut(s) 132
SetI ASST 9 cut(s) 32, 97, 109, 213, 316, 388, 435, 540, 574
SfaNI GCATC 1 cut(s) 353
SfcI CTRYAG 3 cut(s) 83, 261, 539
SfiI GGCCNNNNNGGCC 1 cut(s) 623
SinI GGWCC 2 cut(s) 58, 95
SpeI ACTAGT 1 cut(s) 194
Sse9I AATT 3 cut(s) 7, 361, 583
SseBI AGGCCT 2 cut(s) 161, 188
SspMI CTAG 1 cut(s) 195
StuI AGGCCT 2 cut(s) 161, 188
StyD4I CCNGG 1 cut(s) 130
TaaI ACNGT 1 cut(s) 87
TasI AATT 3 cut(s) 7, 361, 583
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 3 cut(s) 90, 191, 612
Tru9I TTAA 3 cut(s) 90, 191, 612
TscAI CASTG 1 cut(s) 225
TseI GCWGC 2 cut(s) 512, 538
TspDTI ATGAA 1 cut(s) 303
TspRI CASTG 1 cut(s) 225
Van91I CCANNNNNTGG 1 cut(s) 137
VpaK11BI GGWCC 2 cut(s) 58, 95
XapI RAATTY 1 cut(s) 361
XceI RCATGY 1 cut(s) 610
XmnI GAANNNNTTC 1 cut(s) 10
XspI CTAG 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.