Rmu_sc0003539.1_g000006

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003539.1
OG0005824
Physical Location & Seq
Forward (+)
8576 .. 10246
1671 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1671 bp
atgtacactaaattaggggatctgaaggaagtaaggaaccaatttgagctcataagcaatcaggacaatgtatcttggaatgcaattattgttggatatgtgcaggaagaagacgaggacaaggctttttgtatgttcagaagaatgatattgcataggattgtgcctgatgaagtatccttggctagtatactacgtgcttgtgcaaatgttcaagcactggacatgggacagccagtccactgtctctcgattaaaattggtctagaaacaagcctttatgctgggagctcccttattgatatgtattccaaatgtgggctaattagggatgcacgaagtgttcttgatttcttgccccattgcagtgtggtctcgatgaatgctctgatttctggctttgttcatagaaacttcgaagaagcagtaaacatatttcgtgagatgcaggatattggattgaacccatctgaagttactttttcaagtcttttagatgcatgcaatggacctattatgctaccactgggaagacaaatccacaacatagtactaaagaaaggccttttgtttgatggtgacttcttaggtgtctctcttttgggaatgtatatgaattgccaaagaaaaatagatgcaaccagtcttttcttagaattcccaaagccaaaaagcaaagtattatggacctctatgatttcaggactcagtcaaaatgattgtatagaggaggcattgcagttgtatcgagaaatgcgtagtgataatgccctaccagaccaagcaacttttgccagtgttcttcgagcatgttctgttatatcttctttacaaaatggtagagagatccattctctcatttttcatactggttttaatttggatgagttaacatgtagtgccctggtagacatgtatgctaaatgtggggatgtgagaagctctgtgcaagttttccaagaaatgggtactaagaatggtgttatttcttggaactcaatgatcattggttttgctaaaaatgggtatgcagaagatgcatttaagatctttgatgagatgaagcaatctcatgttgagcctgacgacgtcacatttcttggtgttctcactgcatgtagtcatgcagggaaggtggctgaaggtagtgagatctttgattctatggtcaattattattcgattcaacccagggttgatcgtatttgctgcatgcttgatcttctgggcagatggggtttgcttaaagaagcagaggagtttattgataggctagattttgtaccaaattctatgatttgggccacactgcttggtgcttgcaaactacatggagaagacataaggggtcggcgtgcggctaagaaactgaaggagttagaaccacaaagttcgtccccatatgtgctactttctagtatgtgtgctgaatctggaaactggaatgaggctaactctttgaggagggaaatgaaagaaaaaggggtgattaagttgcctggatttagttggatttctgtgggacagaggacaaattactttgttgttggggacaagtctcatccaagtgctgctgaaattcatgtcgctttaaagtacttgacagcaataatgaaaggagagggttgtgtttttgatgaaacagattctttgttgcatgaagaagagtga

Protein Analysis

556

Amino Acids

62.14

Weight (kDa)

5.27

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 22 - 70 1.9e-07 PPR repeat family
PPR_2 PF13041 123 - 168 8.3e-10 PPR repeat family
PPR_2 PF13041 226 - 271 1.9e-09 PPR repeat family
PPR PF01535 227 - 255 1.3e-07 PPR repeat
PPR_3 PF13812 323 - 373 9.4e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 327 - 374 8.4e-13 PPR repeat family
PPR PF01535 329 - 357 5.9e-09 PPR repeat
E_motif PF20431 449 - 505 4.5e-16 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1092
AccB7I CCANNNNNTGG 1 cut(s) 964
AccI GTMKAC 2 cut(s) 190, 909
AciI CCGC 1 cut(s) 1358
AclWI GGATC 2 cut(s) 27, 841
AcsI RAATTY 3 cut(s) 656, 1288, 1578
AcuI CTGAAG 4 cut(s) 44, 492, 1161, 1391
AcyI GRCGYC 1 cut(s) 1089
AdeI CACNNNGTG 1 cut(s) 341
AfaI GTAC 5 cut(s) 5, 552, 970, 1284, 1598
AfiI CCNNNNNNNGG 2 cut(s) 318, 964
AflIII ACRYGT 2 cut(s) 893, 912
AgsI TTSAA 4 cut(s) 215, 463, 486, 1187
AhdI GACNNNNNGTC 1 cut(s) 236
AjnI CCWGG 3 cut(s) 903, 1190, 1498
AluBI AGCT 3 cut(s) 49, 291, 942
AluI AGCT 3 cut(s) 49, 291, 942
Alw21I GWGCWC 2 cut(s) 51, 293
Alw26I GTCTC 4 cut(s) 251, 379, 598, 1563
AlwI GGATC 2 cut(s) 27, 841
AoxI GGCC 2 cut(s) 562, 1302
ApeKI GCWGC 2 cut(s) 1209, 1571
ApoI RAATTY 3 cut(s) 656, 1288, 1578
AspS9I GGNCC 3 cut(s) 509, 687, 1302
AsuHPI GGTGA 2 cut(s) 590, 1498
AsuII TTCGAA 1 cut(s) 417
AvaII GGWCC 2 cut(s) 509, 687
BaeGI GKGCMC 1 cut(s) 904
BanII GRGCYC 2 cut(s) 51, 293
BbsI GAAGAC 3 cut(s) 117, 538, 1344
Bbv12I GWGCWC 2 cut(s) 51, 293
BbvI GCAGC 2 cut(s) 1196, 1558
BccI CCATC 3 cut(s) 475, 569, 1227
BcgI CGANNNNNNTGC 2 cut(s) 728, 762
BciT130I CCWGG 3 cut(s) 905, 1192, 1500
BciVI GTATCC 1 cut(s) 187
BclI TGATCA 1 cut(s) 1002
BcoDI GTCTC 4 cut(s) 251, 379, 598, 1563
BfaI CTAG 4 cut(s) 186, 266, 1274, 1416
BfuI GTATCC 1 cut(s) 187
BglII AGATCT 2 cut(s) 1047, 1152
BisI GCNGC 3 cut(s) 1210, 1359, 1572
BlsI GCNGC 3 cut(s) 1211, 1360, 1573
BmcAI AGTACT 2 cut(s) 552, 1598
Bme1390I CCNGG 3 cut(s) 905, 1192, 1500
Bme18I GGWCC 2 cut(s) 509, 687
BmeRI GACNNNNNGTC 1 cut(s) 236
BmgT120I GGNCC 3 cut(s) 509, 687, 1302
BmiI GGNNCC 1 cut(s) 38
BmrFI CCNGG 3 cut(s) 905, 1192, 1500
BmrI ACTGGG 1 cut(s) 536
BmsI GCATC 5 cut(s) 322, 435, 487, 625, 1027
BmuI ACTGGG 1 cut(s) 536
BpiI GAAGAC 3 cut(s) 117, 538, 1344
BplI GAGNNNNNCTC 2 cut(s) 1439, 1471
Bpu14I TTCGAA 1 cut(s) 417
BsaAI YACGTR 1 cut(s) 197
BsaHI GRCGYC 1 cut(s) 1089
BsaI GGTCTC 1 cut(s) 379
BsaJI CCNNGG 4 cut(s) 180, 903, 1190, 1191
Bsc4I CCNNNNNNNGG 2 cut(s) 318, 964
Bse1I ACTGG 7 cut(s) 225, 236, 531, 642, 795, 874, 1445
Bse3DI GCAATG 3 cut(s) 361, 511, 734
BseBI CCWGG 3 cut(s) 905, 1192, 1500
BseDI CCNNGG 4 cut(s) 180, 903, 1190, 1191
BseGI GGATG 4 cut(s) 337, 889, 937, 1561
BseLI CCNNNNNNNGG 2 cut(s) 318, 964
BseMI GCAATG 3 cut(s) 361, 511, 734
BseMII CTCAG 1 cut(s) 721
BseNI ACTGG 7 cut(s) 225, 236, 531, 642, 795, 874, 1445
BseRI GAGGAG 3 cut(s) 743, 1271, 1477
BseSI GKGCMC 1 cut(s) 904
BseXI GCAGC 2 cut(s) 1196, 1558
BseYI CCCAGC 1 cut(s) 284
BsgI GTGCAG 1 cut(s) 122
BshFI GGCC 2 cut(s) 564, 1304
BsiHKAI GWGCWC 2 cut(s) 51, 293
BslFI GGGAC 4 cut(s) 243, 1381, 1536, 1565
BslI CCNNNNNNNGG 2 cut(s) 318, 964
BsmAI GTCTC 4 cut(s) 251, 379, 598, 1563
BsmFI GGGAC 4 cut(s) 243, 1381, 1536, 1565
BsmI GAATGC 2 cut(s) 85, 388
BsnI GGCC 2 cut(s) 564, 1304
Bso31I GGTCTC 1 cut(s) 379
Bsp119I TTCGAA 1 cut(s) 417
Bsp1286I GDGCHC 3 cut(s) 51, 293, 904
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 7 cut(s) 19, 846, 1002, 1047, 1152, 1198, 1219
BspACI CCGC 1 cut(s) 1358
BspANI GGCC 2 cut(s) 564, 1304
BspCNI CTCAG 1 cut(s) 720
BspLI GGNNCC 1 cut(s) 38
BspPI GGATC 2 cut(s) 27, 841
BspT104I TTCGAA 1 cut(s) 417
BspTNI GGTCTC 1 cut(s) 379
BsrDI GCAATG 3 cut(s) 361, 511, 734
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 7 cut(s) 225, 236, 531, 642, 795, 874, 1445
BssECI CCNNGG 4 cut(s) 180, 903, 1190, 1191
BssMI GATC 7 cut(s) 19, 846, 1002, 1047, 1152, 1198, 1219
BssNAI GTATAC 1 cut(s) 191
BssNI GRCGYC 1 cut(s) 1089
BssT1I CCWWGG 1 cut(s) 180
Bst1107I GTATAC 1 cut(s) 191
Bst2UI CCWGG 3 cut(s) 905, 1192, 1500
Bst4CI ACNGT 1 cut(s) 245
Bst6I CTCTTC 1 cut(s) 1659
BstACI GRCGYC 1 cut(s) 1089
BstAPI GCANNNNNTGC 2 cut(s) 791, 1037
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 197
BstBI TTCGAA 1 cut(s) 417
BstC8I GCNNGC 4 cut(s) 502, 1214, 1321, 1356
BstDEI CTNAG 5 cut(s) 586, 652, 707, 972, 1362
BstF5I GGATG 4 cut(s) 337, 889, 937, 1561
BstKTI GATC 7 cut(s) 22, 849, 1005, 1050, 1155, 1201, 1222
BstMAI GTCTC 4 cut(s) 251, 379, 598, 1563
BstMBI GATC 7 cut(s) 19, 846, 1002, 1047, 1152, 1198, 1219
BstMWI GCNNNNNNNGC 2 cut(s) 791, 1037
BstNI CCWGG 3 cut(s) 905, 1192, 1500
BstNSI RCATGY 6 cut(s) 504, 813, 897, 916, 1119, 1216
BstSCI CCNGG 3 cut(s) 903, 1190, 1498
BstSLI GKGCMC 1 cut(s) 904
BstV1I GCAGC 2 cut(s) 1196, 1558
BstV2I GAAGAC 3 cut(s) 117, 538, 1344
BstX2I RGATCY 4 cut(s) 19, 846, 1047, 1152
BstYI RGATCY 4 cut(s) 19, 846, 1047, 1152
BstZ17I GTATAC 1 cut(s) 191
BsuI GTATCC 1 cut(s) 187
BsuRI GGCC 2 cut(s) 564, 1304
BtsCI GGATG 4 cut(s) 337, 889, 937, 1561
BtsI GCAGTG 3 cut(s) 373, 1110, 1307
BtsIMutI CAGTG 7 cut(s) 218, 241, 373, 524, 802, 1110, 1307
Cac8I GCNNGC 4 cut(s) 502, 1214, 1321, 1356
Cfr13I GGNCC 3 cut(s) 509, 687, 1302
Csp6I GTAC 5 cut(s) 4, 551, 969, 1283, 1597
CspCI CAANNNNNGTGG 2 cut(s) 1294, 1329
CviQI GTAC 5 cut(s) 4, 551, 969, 1283, 1597
DdeI CTNAG 5 cut(s) 586, 652, 707, 972, 1362
DpnI GATC 7 cut(s) 21, 848, 1004, 1049, 1154, 1200, 1221
DpnII GATC 7 cut(s) 19, 846, 1002, 1047, 1152, 1198, 1219
DraI TTTAAA 1 cut(s) 1593
DraIII CACNNNGTG 1 cut(s) 341
DriI GACNNNNNGTC 1 cut(s) 236
Eam1104I CTCTTC 1 cut(s) 1659
Eam1105I GACNNNNNGTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 1659
Ecl136II GAGCTC 2 cut(s) 49, 291
Eco130I CCWWGG 1 cut(s) 180
Eco147I AGGCCT 1 cut(s) 564
Eco24I GRGCYC 2 cut(s) 51, 293
Eco31I GGTCTC 1 cut(s) 379
Eco47I GGWCC 2 cut(s) 509, 687
Eco53kI GAGCTC 2 cut(s) 49, 291
Eco57I CTGAAG 4 cut(s) 44, 492, 1161, 1391
EcoICRI GAGCTC 2 cut(s) 49, 291
EcoRI GAATTC 1 cut(s) 656
EcoRII CCWGG 3 cut(s) 903, 1190, 1498
EcoT14I CCWWGG 1 cut(s) 180
EcoT22I ATGCAT 2 cut(s) 502, 1042
EcoT38I GRGCYC 2 cut(s) 51, 293
ErhI CCWWGG 1 cut(s) 180
FaqI GGGAC 4 cut(s) 243, 1381, 1536, 1565
FauNDI CATATG 1 cut(s) 1402
FbaI TGATCA 1 cut(s) 1002
FblI GTMKAC 2 cut(s) 190, 909
Fnu4HI GCNGC 3 cut(s) 1210, 1359, 1572
FokI GGATG 4 cut(s) 344, 896, 944, 1548
FriOI GRGCYC 2 cut(s) 51, 293
Fsp4HI GCNGC 3 cut(s) 1210, 1359, 1572
FspBI CTAG 4 cut(s) 186, 266, 1274, 1416
GluI GCNGC 3 cut(s) 1210, 1359, 1572
GsaI CCCAGC 1 cut(s) 288
HaeIII GGCC 2 cut(s) 564, 1304
Hin1I GRCGYC 1 cut(s) 1089
HincII GTYRAC 1 cut(s) 891
HindII GTYRAC 1 cut(s) 891
HinfI GANTC 5 cut(s) 705, 1160, 1183, 1430, 1646
HpaI GTTAAC 1 cut(s) 891
HphI GGTGA 2 cut(s) 590, 1498
Hpy166II GTNNAC 6 cut(s) 6, 191, 241, 430, 891, 910
Hpy188I TCNGA 4 cut(s) 24, 140, 390, 472
Hpy188III TCNNGA 9 cut(s) 62, 250, 266, 347, 376, 440, 702, 749, 1434
Hpy8I GTNNAC 6 cut(s) 6, 191, 241, 430, 891, 910
Hpy99I CGWCG 1 cut(s) 1091
HpyAV CCTTC 4 cut(s) 19, 1126, 1136, 1366
HpyCH4III ACNGT 1 cut(s) 245
HpyCH4IV ACGT 2 cut(s) 196, 1089
HpyF10VI GCNNNNNNNGC 2 cut(s) 791, 1037
HpyF3I CTNAG 5 cut(s) 586, 652, 707, 972, 1362
HpySE526I ACGT 2 cut(s) 196, 1089
Hsp92I GRCGYC 1 cut(s) 1089
Ksp22I TGATCA 1 cut(s) 1002
KspAI GTTAAC 1 cut(s) 891
Kzo9I GATC 7 cut(s) 19, 846, 1002, 1047, 1152, 1198, 1219
LmnI GCTCC 2 cut(s) 288, 296
Lsp1109I GCAGC 2 cut(s) 1196, 1558
LweI GCATC 5 cut(s) 322, 435, 487, 625, 1027
MaeI CTAG 4 cut(s) 186, 266, 1274, 1416
MaeII ACGT 2 cut(s) 196, 1089
MaeIII GTNAC 3 cut(s) 475, 578, 1090
MalI GATC 7 cut(s) 21, 848, 1004, 1049, 1154, 1200, 1221
MboI GATC 7 cut(s) 19, 846, 1002, 1047, 1152, 1198, 1219
MflI RGATCY 4 cut(s) 19, 846, 1047, 1152
MhlI GDGCHC 3 cut(s) 51, 293, 904
MlyI GAGTC 1 cut(s) 699
MmeI TCCRAC 2 cut(s) 73, 1490
Mph1103I ATGCAT 2 cut(s) 502, 1042
MseI TTAA 7 cut(s) 255, 876, 890, 1044, 1245, 1491, 1592
MslI CAYNNNNRTG 2 cut(s) 366, 1566
MspR9I CCNGG 3 cut(s) 905, 1192, 1500
Mva1269I GAATGC 2 cut(s) 85, 388
MvaI CCWGG 3 cut(s) 905, 1192, 1500
MwoI GCNNNNNNNGC 2 cut(s) 791, 1037
NdeI CATATG 1 cut(s) 1402
NdeII GATC 7 cut(s) 19, 846, 1002, 1047, 1152, 1198, 1219
NlaIV GGNNCC 1 cut(s) 38
NmuCI GTSAC 2 cut(s) 578, 1090
NsiI ATGCAT 2 cut(s) 502, 1042
NspI RCATGY 6 cut(s) 504, 813, 897, 916, 1119, 1216
NspV TTCGAA 1 cut(s) 417
PaeI GCATGC 2 cut(s) 504, 1216
PasI CCCWGGG 1 cut(s) 1191
PceI AGGCCT 1 cut(s) 564
PciI ACATGT 2 cut(s) 893, 912
PcsI WCGNNNNNNNCGW 1 cut(s) 754
PctI GAATGC 2 cut(s) 85, 388
PfeI GAWTC 4 cut(s) 1160, 1183, 1430, 1646
PflFI GACNNNGTC 2 cut(s) 708, 1088
PflMI CCANNNNNTGG 1 cut(s) 964
PkrI GCNGC 3 cut(s) 1211, 1360, 1573
PleI GAGTC 1 cut(s) 699
PpsI GAGTC 1 cut(s) 699
Ppu21I YACGTR 1 cut(s) 197
PscI ACATGT 2 cut(s) 893, 912
Psp124BI GAGCTC 2 cut(s) 51, 293
Psp6I CCWGG 3 cut(s) 903, 1190, 1498
PspFI CCCAGC 1 cut(s) 284
PspGI CCWGG 3 cut(s) 903, 1190, 1498
PspN4I GGNNCC 1 cut(s) 38
PspPI GGNCC 3 cut(s) 509, 687, 1302
PsuI RGATCY 4 cut(s) 19, 846, 1047, 1152
PsyI GACNNNGTC 2 cut(s) 708, 1088
RsaI GTAC 5 cut(s) 5, 552, 970, 1284, 1598
RsaNI GTAC 5 cut(s) 4, 551, 969, 1283, 1597
RseI CAYNNNNRTG 2 cut(s) 366, 1566
SacI GAGCTC 2 cut(s) 51, 293
SaqAI TTAA 7 cut(s) 255, 876, 890, 1044, 1245, 1491, 1592
SatI GCNGC 3 cut(s) 1210, 1359, 1572
Sau3AI GATC 7 cut(s) 19, 846, 1002, 1047, 1152, 1198, 1219
Sau96I GGNCC 3 cut(s) 509, 687, 1302
ScaI AGTACT 2 cut(s) 552, 1598
SchI GAGTC 1 cut(s) 699
ScrFI CCNGG 3 cut(s) 905, 1192, 1500
SduI GDGCHC 3 cut(s) 51, 293, 904
SfaNI GCATC 5 cut(s) 322, 435, 487, 625, 1027
SfuI TTCGAA 1 cut(s) 417
SinI GGWCC 2 cut(s) 509, 687
SmiMI CAYNNNNRTG 2 cut(s) 366, 1566
SphI GCATGC 2 cut(s) 504, 1216
SseBI AGGCCT 1 cut(s) 564
SsiI CCGC 1 cut(s) 1358
SspMI CTAG 4 cut(s) 186, 266, 1274, 1416
SstI GAGCTC 2 cut(s) 51, 293
StuI AGGCCT 1 cut(s) 564
StyD4I CCNGG 3 cut(s) 903, 1190, 1498
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 1 cut(s) 245
TaiI ACGT 2 cut(s) 199, 1092
TaqI TCGA 6 cut(s) 251, 377, 417, 748, 805, 1181
TatI WGTACW 3 cut(s) 3, 550, 1596
TauI GCSGC 1 cut(s) 1361
TfiI GAWTC 4 cut(s) 1160, 1183, 1430, 1646
Tru1I TTAA 7 cut(s) 255, 876, 890, 1044, 1245, 1491, 1592
Tru9I TTAA 7 cut(s) 255, 876, 890, 1044, 1245, 1491, 1592
TscAI CASTG 7 cut(s) 225, 248, 373, 531, 802, 1117, 1314
TseFI GTSAC 2 cut(s) 578, 1090
TseI GCWGC 2 cut(s) 1209, 1571
Tsp45I GTSAC 2 cut(s) 578, 1090
TspRI CASTG 7 cut(s) 225, 248, 373, 531, 802, 1117, 1314
Tth111I GACNNNGTC 2 cut(s) 708, 1088
Van91I CCANNNNNTGG 1 cut(s) 964
VpaK11BI GGWCC 2 cut(s) 509, 687
XapI RAATTY 3 cut(s) 656, 1288, 1578
XbaI TCTAGA 1 cut(s) 265
XceI RCATGY 6 cut(s) 504, 813, 897, 916, 1119, 1216
XmiI GTMKAC 2 cut(s) 190, 909
XspI CTAG 4 cut(s) 186, 266, 1274, 1416
ZraI GACGTC 1 cut(s) 1090
ZrmI AGTACT 2 cut(s) 552, 1598
Zsp2I ATGCAT 2 cut(s) 502, 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.