Rmu_sc0003601.1_g000003

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003601.1
OG0010551
Physical Location & Seq
Reverse (-)
10131 .. 14189
4059 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1626 bp
atgagcggcggtggggagactagggtttcaatcccagacaatgtacacagcacgatccaaaacatcagagagatcactaagaagcttcacagcgacgatgaaatctatgctgtgctcaaggagtgctctatggatcccaacgagaccgcccagaagctcttgtatttagatacatttcatgaggtcaaaaagagacgtgatcggagaaaggagaacctgaaaggcagggcatctgaagattcaaggacagcaccaggtgcgcacaggcgaatggtcaggggtggtggccaagggaattattcttctgatgctggtggtgggaggaattcttctgctcggagggaaaatggagccagtcatgttgcagatggaggttctctgtcctcttttatgccaggtcagcagaaaactaagaacattgctgcggctccaatcgcaaaagcttcaattggcattccaaacgggattaaaaacctgtcaaatgggacttctagtcatgtttctgctccaaaatcctctgccggtgtagatgcaaacaagttgggagctacagcacctcgttatgctgtggttgtggcatctacacctgttgttggcttaaaggatcaacgcaaggccccaacaactacatctgatcatcttccaaattctgccacttcagcatctgattctggtgtttactcatctgcttcagatccttccttatcaactcatataccagaaaacaaagatgtttctggtgaatctgttgtcatagagttgtcaaagagtgaaaatgttgcctccaaatctgataatcacgtgatcaaagaaaagactcaaagtgacaatgctaaggcaattggaaagaatcggcatactaagtcgtcagaacccccttcctcaactcatgatggttctcaggccattctgccttctaattctgatggagaatcacagatagagtcgactgatgctttgaaagtggccaaactggaggttgaaacagttgctgttaaaccttcttctcagttgccacctgactcgagtctctcagataccaaacttgttacttttccaaatcatatcaaggtccctgaatctttaagaaatgttttgacttttggaagtattaattctacttttggaacgagtgttgactgcgttaacggtactggtggcaatggttctacgggcggtgtagaatcttctcaggatactgaagaaactgctaaagagccttctcctagcaataacagtgtagcctcatctgttcaaggagatttttcagaggatccactgccttcaccaaatgttcttgaaaagttgcaaccacctgagggtaatgtctcttcagagagtaatgtcccttcagcccctgactcagactccaaaagtgaactgttgaagcaggatttgcagttgcctccagaagttccacataaccttactgttgaagagaatgaactccgcgagaacaatggtaggagtgacagcaattttagccatctggtagaaaccaacactgcaattaatgagcagaatggtgaggaaagtagacaacaacatattgagtttataacaattttgtacatcacctgttgtgttttaggcttgtattggcaagaccagaagcaaaagccatga

Protein Analysis

541

Amino Acids

57.48

Weight (kDa)

5.6

Isoelectric Point (pI)

55.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 11 - 71 1.3e-30 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1559
Acc16I TGCGCA 1 cut(s) 261
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 2 cut(s) 947, 1537
AccII CGCG 1 cut(s) 1452
AciI CCGC 6 cut(s) 6, 9, 147, 425, 1176, 1450
AclWI GGATC 7 cut(s) 49, 128, 141, 612, 689, 1268, 1281
AcoI YGGCCR 2 cut(s) 286, 966
AcsI RAATTY 2 cut(s) 325, 646
AcuI CTGAAG 6 cut(s) 255, 642, 675, 1221, 1317, 1335
AcvI CACGTG 1 cut(s) 802
AdeI CACNNNGTG 1 cut(s) 257
AfaI GTAC 3 cut(s) 45, 1153, 1571
AfiI CCNNNNNNNGG 2 cut(s) 593, 1319
AgsI TTSAA 9 cut(s) 30, 243, 447, 961, 983, 1256, 1301, 1387, 1436
AhdI GACNNNNNGTC 1 cut(s) 492
AjiI CACGTC 1 cut(s) 197
AjnI CCWGG 2 cut(s) 253, 394
AluBI AGCT 4 cut(s) 85, 157, 443, 548
AluI AGCT 4 cut(s) 85, 157, 443, 548
Alw21I GWGCWC 2 cut(s) 117, 128
Alw26I GTCTC 5 cut(s) 11, 137, 187, 1034, 1333
AlwI GGATC 7 cut(s) 49, 128, 141, 612, 689, 1268, 1281
AlwNI CAGNNNCTG 3 cut(s) 665, 992, 1358
Ama87I CYCGRG 1 cut(s) 1024
AoxI GGCC 4 cut(s) 286, 615, 903, 966
ApeKI GCWGC 1 cut(s) 422
ApoI RAATTY 2 cut(s) 325, 646
ArsI GACNNNNNNTTYG 2 cut(s) 940, 972
AseI ATTAAT 2 cut(s) 1113, 1512
AspLEI GCGC 1 cut(s) 262
AspS9I GGNCC 2 cut(s) 616, 1072
AsuHPI GGTGA 4 cut(s) 752, 1278, 1538, 1567
AvaI CYCGRG 1 cut(s) 1024
AvaII GGWCC 1 cut(s) 1072
AxyI CCTNAGG 1 cut(s) 1317
BalI TGGCCA 2 cut(s) 288, 968
BamHI GGATCC 2 cut(s) 133, 1273
BarI GAAGNNNNNNTAC 4 cut(s) 1315, 1333, 1347, 1365
BbrPI CACGTG 1 cut(s) 802
Bbv12I GWGCWC 2 cut(s) 117, 128
BbvI GCAGC 1 cut(s) 409
BccI CCATC 4 cut(s) 362, 887, 920, 1494
BciT130I CCWGG 2 cut(s) 255, 396
BciVI GTATCC 1 cut(s) 1189
BclI TGATCA 2 cut(s) 634, 804
BcoDI GTCTC 5 cut(s) 11, 137, 187, 1034, 1333
BfaI CTAG 3 cut(s) 21, 492, 1227
BfmI CTRYAG 1 cut(s) 549
BfuI GTATCC 1 cut(s) 1189
BisI GCNGC 3 cut(s) 7, 423, 426
BlsI GCNGC 3 cut(s) 8, 424, 427
Bme1390I CCNGG 2 cut(s) 255, 396
Bme18I GGWCC 1 cut(s) 1072
BmeRI GACNNNNNGTC 1 cut(s) 492
BmeT110I CYCGRG 1 cut(s) 1024
BmgBI CACGTC 1 cut(s) 197
BmgT120I GGNCC 2 cut(s) 616, 1072
BmiI GGNNCC 6 cut(s) 135, 352, 429, 618, 1074, 1275
BmrFI CCNGG 2 cut(s) 255, 396
BmsI GCATC 6 cut(s) 239, 298, 520, 587, 671, 943
BoxI GACNNNNGTC 1 cut(s) 1026
BpmI CTGGAG 2 cut(s) 995, 1392
Bpu10I CCTNAGC 1 cut(s) 834
BpuEI CTTGAG 1 cut(s) 101
BsaAI YACGTR 1 cut(s) 802
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 1 cut(s) 289
BsaXI ACNNNNNCTCC 2 cut(s) 1352, 1382
Bsc4I CCNNNNNNNGG 2 cut(s) 593, 1319
Bse118I RCCGGY 1 cut(s) 521
Bse1I ACTGG 3 cut(s) 354, 978, 1159
Bse21I CCTNAGG 1 cut(s) 1317
Bse3DI GCAATG 2 cut(s) 417, 1168
BseBI CCWGG 2 cut(s) 255, 396
BseDI CCNNGG 1 cut(s) 289
BseLI CCNNNNNNNGG 2 cut(s) 593, 1319
BseMI GCAATG 2 cut(s) 417, 1168
BseMII CTCAG 6 cut(s) 914, 1022, 1047, 1205, 1308, 1377
BseNI ACTGG 3 cut(s) 354, 978, 1159
BseXI GCAGC 1 cut(s) 409
Bsh1236I CGCG 1 cut(s) 1452
BshFI GGCC 4 cut(s) 288, 617, 905, 968
BsiHKAI GWGCWC 2 cut(s) 117, 128
BsiHKCI CYCGRG 1 cut(s) 1024
BsiSI CCGG 1 cut(s) 522
BslFI GGGAC 3 cut(s) 499, 1058, 1331
BslI CCNNNNNNNGG 2 cut(s) 593, 1319
BsmAI GTCTC 5 cut(s) 11, 137, 187, 1034, 1333
BsmBI CGTCTC 1 cut(s) 187
BsmFI GGGAC 3 cut(s) 499, 1058, 1331
BsmI GAATGC 1 cut(s) 453
BsnI GGCC 4 cut(s) 288, 617, 905, 968
Bso31I GGTCTC 1 cut(s) 137
BsoBI CYCGRG 1 cut(s) 1024
Bsp1286I GDGCHC 2 cut(s) 117, 128
Bsp1407I TGTACA 2 cut(s) 43, 1569
Bsp143I GATC 9 cut(s) 54, 72, 133, 199, 604, 634, 694, 804, 1273
BspACI CCGC 6 cut(s) 6, 9, 147, 425, 1176, 1450
BspANI GGCC 4 cut(s) 288, 617, 905, 968
BspCNI CTCAG 6 cut(s) 913, 1021, 1046, 1204, 1309, 1376
BspFNI CGCG 1 cut(s) 1452
BspHI TCATGA 2 cut(s) 178, 889
BspLI GGNNCC 6 cut(s) 135, 352, 429, 618, 1074, 1275
BspPI GGATC 7 cut(s) 49, 128, 141, 612, 689, 1268, 1281
BspTNI GGTCTC 1 cut(s) 137
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 2 cut(s) 417, 1168
BsrFI RCCGGY 1 cut(s) 521
BsrGI TGTACA 2 cut(s) 43, 1569
BsrI ACTGG 3 cut(s) 354, 978, 1159
BssAI RCCGGY 1 cut(s) 521
BssECI CCNNGG 1 cut(s) 289
BssMI GATC 9 cut(s) 54, 72, 133, 199, 604, 634, 694, 804, 1273
BssT1I CCWWGG 1 cut(s) 289
Bst2UI CCWGG 2 cut(s) 255, 396
Bst4CI ACNGT 5 cut(s) 988, 1151, 1238, 1383, 1432
Bst6I CTCTTC 2 cut(s) 1336, 1431
BstAPI GCANNNNNTGC 2 cut(s) 257, 1396
BstAUI TGTACA 2 cut(s) 43, 1569
BstBAI YACGTR 1 cut(s) 802
BstFNI CGCG 1 cut(s) 1452
BstHHI GCGC 1 cut(s) 262
BstKTI GATC 9 cut(s) 57, 75, 136, 202, 607, 637, 697, 807, 1276
BstMAI GTCTC 5 cut(s) 11, 137, 187, 1034, 1333
BstMBI GATC 9 cut(s) 54, 72, 133, 199, 604, 634, 694, 804, 1273
BstMWI GCNNNNNNNGC 6 cut(s) 257, 400, 434, 659, 1396, 1482
BstNI CCWGG 2 cut(s) 255, 396
BstPAI GACNNNNGTC 1 cut(s) 1026
BstSCI CCNGG 2 cut(s) 253, 394
BstSFI CTRYAG 1 cut(s) 549
BstUI CGCG 1 cut(s) 1452
BstV1I GCAGC 1 cut(s) 409
BstX2I RGATCY 3 cut(s) 133, 694, 1273
BstYI RGATCY 3 cut(s) 133, 694, 1273
Bsu36I CCTNAGG 1 cut(s) 1317
BsuI GTATCC 1 cut(s) 1189
BsuRI GGCC 4 cut(s) 288, 617, 905, 968
BtrI CACGTC 1 cut(s) 197
BtsI GCAGTG 2 cut(s) 1277, 1503
BtsIMutI CAGTG 3 cut(s) 1243, 1277, 1503
CaiI CAGNNNCTG 3 cut(s) 665, 992, 1358
CciI TCATGA 2 cut(s) 178, 889
CfoI GCGC 1 cut(s) 262
Cfr10I RCCGGY 1 cut(s) 521
Cfr13I GGNCC 2 cut(s) 616, 1072
CsiI ACCWGGT 1 cut(s) 253
Csp6I GTAC 3 cut(s) 44, 1152, 1570
CviAII CATG 5 cut(s) 179, 359, 497, 890, 1623
CviQI GTAC 3 cut(s) 44, 1152, 1570
DpnI GATC 9 cut(s) 56, 74, 135, 201, 606, 636, 696, 806, 1275
DpnII GATC 9 cut(s) 54, 72, 133, 199, 604, 634, 694, 804, 1273
DraIII CACNNNGTG 1 cut(s) 257
DriI GACNNNNNGTC 1 cut(s) 492
EaeI YGGCCR 2 cut(s) 286, 966
Eam1104I CTCTTC 2 cut(s) 1336, 1431
Eam1105I GACNNNNNGTC 1 cut(s) 492
EarI CTCTTC 2 cut(s) 1336, 1431
Eco130I CCWWGG 1 cut(s) 289
Eco31I GGTCTC 1 cut(s) 137
Eco47I GGWCC 1 cut(s) 1072
Eco57I CTGAAG 6 cut(s) 255, 642, 675, 1221, 1317, 1335
Eco72I CACGTG 1 cut(s) 802
Eco81I CCTNAGG 1 cut(s) 1317
Eco88I CYCGRG 1 cut(s) 1024
EcoO109I RGGNCCY 2 cut(s) 616, 1072
EcoRI GAATTC 1 cut(s) 325
EcoRII CCWGG 2 cut(s) 253, 394
EcoT14I CCWWGG 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 289
Esp3I CGTCTC 1 cut(s) 187
FaeI CATG 5 cut(s) 182, 362, 500, 893, 1626
FaqI GGGAC 3 cut(s) 499, 1058, 1331
FatI CATG 5 cut(s) 178, 358, 496, 889, 1622
FbaI TGATCA 2 cut(s) 634, 804
FblI GTMKAC 2 cut(s) 947, 1537
Fnu4HI GCNGC 3 cut(s) 7, 423, 426
Fsp4HI GCNGC 3 cut(s) 7, 423, 426
FspAI RTGCGCAY 1 cut(s) 261
FspBI CTAG 3 cut(s) 21, 492, 1227
FspI TGCGCA 1 cut(s) 261
GlaI GCGC 1 cut(s) 261
GluI GCNGC 3 cut(s) 7, 423, 426
GsuI CTGGAG 2 cut(s) 995, 1392
HaeIII GGCC 4 cut(s) 288, 617, 905, 968
HapII CCGG 1 cut(s) 522
HhaI GCGC 1 cut(s) 262
Hin1II CATG 5 cut(s) 182, 362, 500, 893, 1626
Hin6I GCGC 1 cut(s) 260
HinP1I GCGC 1 cut(s) 260
HincII GTYRAC 3 cut(s) 948, 1138, 1147
HindII GTYRAC 3 cut(s) 948, 1138, 1147
HindIII AAGCTT 2 cut(s) 83, 441
HpaI GTTAAC 1 cut(s) 1147
HpaII CCGG 1 cut(s) 522
HphI GGTGA 4 cut(s) 752, 1278, 1538, 1567
Hpy166II GTNNAC 7 cut(s) 46, 679, 948, 1138, 1147, 1379, 1538
Hpy188III TCNNGA 5 cut(s) 179, 890, 1193, 1298, 1409
Hpy8I GTNNAC 7 cut(s) 46, 679, 948, 1138, 1147, 1379, 1538
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 7 cut(s) 708, 888, 924, 1011, 1230, 1293, 1359
HpyCH4III ACNGT 5 cut(s) 988, 1151, 1238, 1383, 1432
HpyCH4IV ACGT 2 cut(s) 196, 801
HpyCH4V TGCA 5 cut(s) 365, 533, 1309, 1399, 1508
HpyF10VI GCNNNNNNNGC 6 cut(s) 257, 400, 434, 659, 1396, 1482
HpySE526I ACGT 2 cut(s) 196, 801
Hsp92II CATG 5 cut(s) 182, 362, 500, 893, 1626
HspAI GCGC 1 cut(s) 260
Ksp22I TGATCA 2 cut(s) 634, 804
KspAI GTTAAC 1 cut(s) 1147
Kzo9I GATC 9 cut(s) 54, 72, 133, 199, 604, 634, 694, 804, 1273
LmnI GCTCC 4 cut(s) 350, 433, 511, 545
Lsp1109I GCAGC 1 cut(s) 409
LweI GCATC 6 cut(s) 239, 298, 520, 587, 671, 943
MabI ACCWGGT 1 cut(s) 253
MaeI CTAG 3 cut(s) 21, 492, 1227
MaeII ACGT 2 cut(s) 196, 801
MaeIII GTNAC 3 cut(s) 824, 1048, 1469
MalI GATC 9 cut(s) 56, 74, 135, 201, 606, 636, 696, 806, 1275
MbiI CCGCTC 1 cut(s) 6
MboI GATC 9 cut(s) 54, 72, 133, 199, 604, 634, 694, 804, 1273
MboII GAAGA 9 cut(s) 248, 294, 321, 632, 996, 1179, 1214, 1323, 1448
MfeI CAATTG 2 cut(s) 447, 840
MflI RGATCY 3 cut(s) 133, 694, 1273
MhlI GDGCHC 2 cut(s) 117, 128
MlsI TGGCCA 2 cut(s) 288, 968
MluNI TGGCCA 2 cut(s) 288, 968
MlyI GAGTC 6 cut(s) 811, 953, 1016, 1036, 1355, 1361
Mox20I TGGCCA 2 cut(s) 288, 968
MscI TGGCCA 2 cut(s) 288, 968
MseI TTAA 7 cut(s) 468, 599, 996, 1085, 1113, 1146, 1512
Msp20I TGGCCA 2 cut(s) 288, 968
MspI CCGG 1 cut(s) 522
MspR9I CCNGG 2 cut(s) 255, 396
MunI CAATTG 2 cut(s) 447, 840
Mva1269I GAATGC 1 cut(s) 453
MvaI CCWGG 2 cut(s) 255, 396
MvnI CGCG 1 cut(s) 1452
MwoI GCNNNNNNNGC 6 cut(s) 257, 400, 434, 659, 1396, 1482
NdeII GATC 9 cut(s) 54, 72, 133, 199, 604, 634, 694, 804, 1273
NlaIII CATG 5 cut(s) 182, 362, 500, 893, 1626
NlaIV GGNNCC 6 cut(s) 135, 352, 429, 618, 1074, 1275
NmuCI GTSAC 2 cut(s) 824, 1469
NsbI TGCGCA 1 cut(s) 261
PaeR7I CTCGAG 1 cut(s) 1024
PagI TCATGA 2 cut(s) 178, 889
PctI GAATGC 1 cut(s) 453
PfeI GAWTC 7 cut(s) 239, 668, 743, 850, 932, 1079, 1184
PkrI GCNGC 3 cut(s) 8, 424, 427
PleI GAGTC 6 cut(s) 811, 952, 1016, 1035, 1355, 1361
PmaCI CACGTG 1 cut(s) 802
PmlI CACGTG 1 cut(s) 802
PpsI GAGTC 6 cut(s) 811, 952, 1016, 1035, 1355, 1361
Ppu21I YACGTR 1 cut(s) 802
PpuMI RGGWCCY 1 cut(s) 1072
PshAI GACNNNNGTC 1 cut(s) 1026
PshBI ATTAAT 2 cut(s) 1113, 1512
PsiI TTATAA 1 cut(s) 1559
Psp5II RGGWCCY 1 cut(s) 1072
Psp6I CCWGG 2 cut(s) 253, 394
PspCI CACGTG 1 cut(s) 802
PspGI CCWGG 2 cut(s) 253, 394
PspN4I GGNNCC 6 cut(s) 135, 352, 429, 618, 1074, 1275
PspPI GGNCC 2 cut(s) 616, 1072
PspPPI RGGWCCY 1 cut(s) 1072
PspXI VCTCGAGB 1 cut(s) 1024
PstNI CAGNNNCTG 3 cut(s) 665, 992, 1358
PsuI RGATCY 3 cut(s) 133, 694, 1273
RsaI GTAC 3 cut(s) 45, 1153, 1571
RsaNI GTAC 3 cut(s) 44, 1152, 1570
SalI GTCGAC 1 cut(s) 946
SaqAI TTAA 7 cut(s) 468, 599, 996, 1085, 1113, 1146, 1512
SatI GCNGC 3 cut(s) 7, 423, 426
Sau3AI GATC 9 cut(s) 54, 72, 133, 199, 604, 634, 694, 804, 1273
Sau96I GGNCC 2 cut(s) 616, 1072
SchI GAGTC 6 cut(s) 811, 953, 1016, 1036, 1355, 1361
ScrFI CCNGG 2 cut(s) 255, 396
SduI GDGCHC 2 cut(s) 117, 128
SexAI ACCWGGT 1 cut(s) 253
SfaNI GCATC 6 cut(s) 239, 298, 520, 587, 671, 943
SfcI CTRYAG 1 cut(s) 549
Sfr274I CTCGAG 1 cut(s) 1024
SinI GGWCC 1 cut(s) 1072
SlaI CTCGAG 1 cut(s) 1024
SmlI CTYRAG 2 cut(s) 116, 1024
SmoI CTYRAG 2 cut(s) 116, 1024
SsiI CCGC 6 cut(s) 6, 9, 147, 425, 1176, 1450
SspMI CTAG 3 cut(s) 21, 492, 1227
StyD4I CCNGG 2 cut(s) 253, 394
StyI CCWWGG 1 cut(s) 289
TaaI ACNGT 5 cut(s) 988, 1151, 1238, 1383, 1432
TaiI ACGT 2 cut(s) 199, 804
TaqI TCGA 2 cut(s) 947, 1025
TatI WGTACW 2 cut(s) 43, 1569
TauI GCSGC 2 cut(s) 9, 428
TfiI GAWTC 7 cut(s) 239, 668, 743, 850, 932, 1079, 1184
Tru1I TTAA 7 cut(s) 468, 599, 996, 1085, 1113, 1146, 1512
Tru9I TTAA 7 cut(s) 468, 599, 996, 1085, 1113, 1146, 1512
TscAI CASTG 3 cut(s) 1243, 1284, 1510
TseFI GTSAC 2 cut(s) 824, 1469
TseI GCWGC 1 cut(s) 422
Tsp45I GTSAC 2 cut(s) 824, 1469
TspDTI ATGAA 3 cut(s) 114, 167, 1458
TspRI CASTG 3 cut(s) 1243, 1284, 1510
VpaK11BI GGWCC 1 cut(s) 1072
VspI ATTAAT 2 cut(s) 1113, 1512
XapI RAATTY 2 cut(s) 325, 646
XhoI CTCGAG 1 cut(s) 1024
XmiI GTMKAC 2 cut(s) 947, 1537
XspI CTAG 3 cut(s) 21, 492, 1227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.