Rorug05G0445300

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010551
Physical Location & Seq
Forward (+)
61685667 .. 61688128
2462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0445300.1

Sequence Viewer

Length: 762 bp
ATGAGAGATGTATTTGATGATAATGAAGACCTTCAAAACTTGCCACCGAATCTGCCAACGACATTAAAGGATTTGTGCACGTGGGGGAACACTTTCCTTAGAGACGGGGTCACCATCCACACAACATTTGCTGCGGACCTTTTCGGCCATTCAAAGAAAGTCTTTCTGTTTAGAAGTGATCTCTATACCATGGCCAACAAGCTAGAAGTATCAAATCGTGTACTTGTGTTTTACATGAGCTACCTTCATGAAGTCTTGAAGAAGTGTAAGATGGTGGATATGGTTGCCTTTGTCGATCCTGATCATATTGGTACACTTGGGTGTGGAAATCCCACTGAAAGTGCTTGGTCTTTGTCAGATCGGTATAGGCAAGGGAGGTTAGGGCAAATCTTTGTGGTTCCATATAACTTGGGTGCTCATTGGATGTTGACGGTTGTGAACCCAAATGAAGAAGTCGTGCACTTCATGGATCCACTTAAAAGGCGACTCGATACTGGGGAATGGAAATCTATTGTCAACAACTTCATTAAAATCTATAATGCCCACAAGAATCGGAAAGGAAGGAAAGGTATTCTGGAGCAAAAAAATGACAAGACTTGTGGATATTTCATTATGCGTTACATGAAAGAAATTGTGGAGGACAAGAACTTGGATTTTAGCATTAAGTGGGGAACGGGGTCAAATTTGGTTTACACAGACAAAGATATTGATGAGATCCGAGCGGAATGGGCTAAGCATGTTTTGAAGTTCCCAGAAAATTAA

Protein Analysis

253

Amino Acids

29.44

Weight (kDa)

7.69

Isoelectric Point (pI)

25.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 130 - 246 4.7e-08 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 722
AciI CCGC 2 cut(s) 134, 722
AclWI GGATC 4 cut(s) 290, 464, 477, 709
AcoI YGGCCR 2 cut(s) 145, 192
AcsI RAATTY 1 cut(s) 682
AcvI CACGTG 1 cut(s) 81
AfaI GTAC 2 cut(s) 222, 313
AgsI TTSAA 4 cut(s) 35, 153, 259, 745
AleI CACNNNNGTG 1 cut(s) 319
AluBI AGCT 2 cut(s) 202, 240
AluI AGCT 2 cut(s) 202, 240
Alw21I GWGCWC 3 cut(s) 80, 418, 462
Alw26I GTCTC 1 cut(s) 96
Alw44I GTGCAC 2 cut(s) 76, 458
AlwI GGATC 4 cut(s) 290, 464, 477, 709
AoxI GGCC 2 cut(s) 145, 192
ApaLI GTGCAC 2 cut(s) 76, 458
ApeKI GCWGC 1 cut(s) 131
ApoI RAATTY 1 cut(s) 682
Asp700I GAANNNNTTC 2 cut(s) 30, 92
AspS9I GGNCC 1 cut(s) 136
AsuHPI GGTGA 1 cut(s) 103
AvaII GGWCC 1 cut(s) 136
BaeGI GKGCMC 2 cut(s) 80, 462
BalI TGGCCA 1 cut(s) 194
BamHI GGATCC 1 cut(s) 469
BbrPI CACGTG 1 cut(s) 81
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 3 cut(s) 80, 418, 462
BbvI GCAGC 1 cut(s) 118
BccI CCATC 2 cut(s) 122, 265
BclI TGATCA 1 cut(s) 301
BcoDI GTCTC 1 cut(s) 96
BfaI CTAG 1 cut(s) 203
BisI GCNGC 1 cut(s) 132
BlpI GCTNAGC 1 cut(s) 732
BlsI GCNGC 1 cut(s) 133
Bme18I GGWCC 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 2 cut(s) 399, 471
BmrI ACTGGG 1 cut(s) 504
BmuI ACTGGG 1 cut(s) 504
BpiI GAAGAC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 596
Bpu1102I GCTNAGC 1 cut(s) 732
BsaAI YACGTR 1 cut(s) 81
BsaBI GATNNNNATC 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 189
Bse1I ACTGG 1 cut(s) 499
Bse8I GATNNNNATC 1 cut(s) 300
BseDI CCNNGG 1 cut(s) 189
BseGI GGATG 2 cut(s) 114, 429
BseJI GATNNNNATC 1 cut(s) 300
BseNI ACTGG 1 cut(s) 499
BseSI GKGCMC 2 cut(s) 80, 462
BseXI GCAGC 1 cut(s) 118
BshFI GGCC 2 cut(s) 147, 194
BsiHKAI GWGCWC 3 cut(s) 80, 418, 462
BsmAI GTCTC 1 cut(s) 96
BsmBI CGTCTC 1 cut(s) 96
BsnI GGCC 2 cut(s) 147, 194
Bsp1286I GDGCHC 3 cut(s) 80, 418, 462
Bsp143I GATC 6 cut(s) 178, 295, 301, 358, 469, 714
Bsp1720I GCTNAGC 1 cut(s) 732
Bsp19I CCATGG 1 cut(s) 189
BspACI CCGC 2 cut(s) 134, 722
BspANI GGCC 2 cut(s) 147, 194
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 399, 471
BspPI GGATC 4 cut(s) 290, 464, 477, 709
BsrBI CCGCTC 1 cut(s) 722
BsrI ACTGG 1 cut(s) 499
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 6 cut(s) 178, 295, 301, 358, 469, 714
BssT1I CCWWGG 1 cut(s) 189
Bst4CI ACNGT 1 cut(s) 433
BstBAI YACGTR 1 cut(s) 81
BstDEI CTNAG 2 cut(s) 98, 732
BstDSI CCRYGG 1 cut(s) 189
BstEII GGTNACC 1 cut(s) 109
BstF5I GGATG 2 cut(s) 114, 429
BstKTI GATC 6 cut(s) 181, 298, 304, 361, 472, 717
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 6 cut(s) 178, 295, 301, 358, 469, 714
BstMWI GCNNNNNNNGC 1 cut(s) 728
BstNSI RCATGY 1 cut(s) 740
BstPI GGTNACC 1 cut(s) 109
BstSLI GKGCMC 2 cut(s) 80, 462
BstV1I GCAGC 1 cut(s) 118
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 2 cut(s) 469, 714
BstYI RGATCY 2 cut(s) 469, 714
BsuRI GGCC 2 cut(s) 147, 194
BtgI CCRYGG 1 cut(s) 189
BtsCI GGATG 2 cut(s) 114, 429
BtsIMutI CAGTG 1 cut(s) 333
CciI TCATGA 1 cut(s) 247
Cfr13I GGNCC 1 cut(s) 136
Csp6I GTAC 2 cut(s) 221, 312
CspCI CAANNNNNGTGG 2 cut(s) 580, 615
CviAII CATG 6 cut(s) 190, 235, 248, 466, 622, 737
CviJI RGCY 5 cut(s) 147, 194, 202, 240, 731
CviKI_1 RGCY 5 cut(s) 147, 194, 202, 240, 731
CviQI GTAC 2 cut(s) 221, 312
DdeI CTNAG 2 cut(s) 98, 732
DpnI GATC 6 cut(s) 180, 297, 303, 360, 471, 716
DpnII GATC 6 cut(s) 178, 295, 301, 358, 469, 714
EaeI YGGCCR 2 cut(s) 145, 192
Eco130I CCWWGG 1 cut(s) 189
Eco47I GGWCC 1 cut(s) 136
Eco72I CACGTG 1 cut(s) 81
Eco91I GGTNACC 1 cut(s) 109
EcoO65I GGTNACC 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 189
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 6 cut(s) 193, 238, 251, 469, 625, 740
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FatI CATG 6 cut(s) 189, 234, 247, 465, 621, 736
FbaI TGATCA 1 cut(s) 301
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 2 cut(s) 101, 436
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 1 cut(s) 203
GluI GCNGC 1 cut(s) 132
GsuI CTGGAG 1 cut(s) 596
HaeIII GGCC 2 cut(s) 147, 194
Hin1II CATG 6 cut(s) 193, 238, 251, 469, 625, 740
HincII GTYRAC 2 cut(s) 429, 517
HindII GTYRAC 2 cut(s) 429, 517
HinfI GANTC 3 cut(s) 49, 486, 550
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 8 cut(s) 78, 221, 314, 429, 439, 460, 517, 691
Hpy188I TCNGA 3 cut(s) 358, 555, 719
Hpy188III TCNNGA 4 cut(s) 248, 256, 299, 575
Hpy8I GTNNAC 8 cut(s) 78, 221, 314, 429, 439, 460, 517, 691
HpyAV CCTTC 3 cut(s) 41, 254, 555
HpyCH4III ACNGT 1 cut(s) 433
HpyCH4IV ACGT 1 cut(s) 80
HpyCH4V TGCA 2 cut(s) 78, 460
HpyF10VI GCNNNNNNNGC 1 cut(s) 728
HpyF3I CTNAG 2 cut(s) 98, 732
HpySE526I ACGT 1 cut(s) 80
Hsp92II CATG 6 cut(s) 193, 238, 251, 469, 625, 740
Ksp22I TGATCA 1 cut(s) 301
Kzo9I GATC 6 cut(s) 178, 295, 301, 358, 469, 714
LmnI GCTCC 1 cut(s) 577
LpnPI CCDG 3 cut(s) 312, 480, 560
Lsp1109I GCAGC 1 cut(s) 118
MaeI CTAG 1 cut(s) 203
MaeII ACGT 1 cut(s) 80
MaeIII GTNAC 2 cut(s) 109, 617
MalI GATC 6 cut(s) 180, 297, 303, 360, 471, 716
MbiI CCGCTC 1 cut(s) 722
MboI GATC 6 cut(s) 178, 295, 301, 358, 469, 714
MboII GAAGA 3 cut(s) 38, 271, 461
MflI RGATCY 2 cut(s) 469, 714
MhlI GDGCHC 3 cut(s) 80, 418, 462
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 3 cut(s) 630, 682, 757
MluNI TGGCCA 1 cut(s) 194
MlyI GAGTC 1 cut(s) 480
MnlI CCTC 2 cut(s) 369, 631
Mox20I TGGCCA 1 cut(s) 194
MroXI GAANNNNTTC 2 cut(s) 30, 92
MscI TGGCCA 1 cut(s) 194
MseI TTAA 5 cut(s) 65, 477, 528, 663, 760
MslI CAYNNNNRTG 1 cut(s) 319
Msp20I TGGCCA 1 cut(s) 194
MwoI GCNNNNNNNGC 1 cut(s) 728
NcoI CCATGG 1 cut(s) 189
NdeII GATC 6 cut(s) 178, 295, 301, 358, 469, 714
NlaIII CATG 6 cut(s) 193, 238, 251, 469, 625, 740
NlaIV GGNNCC 2 cut(s) 399, 471
NmuCI GTSAC 1 cut(s) 109
NspI RCATGY 1 cut(s) 740
OliI CACNNNNGTG 1 cut(s) 319
PagI TCATGA 1 cut(s) 247
PdmI GAANNNNTTC 2 cut(s) 30, 92
PfeI GAWTC 2 cut(s) 49, 550
PflFI GACNNNGTC 1 cut(s) 107
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 1 cut(s) 480
PmaCI CACGTG 1 cut(s) 81
PmlI CACGTG 1 cut(s) 81
PpsI GAGTC 1 cut(s) 480
Ppu21I YACGTR 1 cut(s) 81
PspCI CACGTG 1 cut(s) 81
PspEI GGTNACC 1 cut(s) 109
PspN4I GGNNCC 2 cut(s) 399, 471
PspPI GGNCC 1 cut(s) 136
PsuI RGATCY 2 cut(s) 469, 714
PsyI GACNNNGTC 1 cut(s) 107
RsaI GTAC 2 cut(s) 222, 313
RsaNI GTAC 2 cut(s) 221, 312
RseI CAYNNNNRTG 1 cut(s) 319
SaqAI TTAA 5 cut(s) 65, 477, 528, 663, 760
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 6 cut(s) 178, 295, 301, 358, 469, 714
Sau96I GGNCC 1 cut(s) 136
SchI GAGTC 1 cut(s) 480
SduI GDGCHC 3 cut(s) 80, 418, 462
SetI ASST 8 cut(s) 33, 83, 141, 204, 242, 246, 380, 571
SinI GGWCC 1 cut(s) 136
SmiMI CAYNNNNRTG 1 cut(s) 319
Sse9I AATT 3 cut(s) 630, 682, 757
SsiI CCGC 2 cut(s) 134, 722
SspMI CTAG 1 cut(s) 203
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 1 cut(s) 433
TaiI ACGT 1 cut(s) 83
TaqI TCGA 2 cut(s) 294, 489
TasI AATT 3 cut(s) 630, 682, 757
TatI WGTACW 1 cut(s) 220
TfiI GAWTC 2 cut(s) 49, 550
Tru1I TTAA 5 cut(s) 65, 477, 528, 663, 760
Tru9I TTAA 5 cut(s) 65, 477, 528, 663, 760
TscAI CASTG 1 cut(s) 340
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 1 cut(s) 131
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 8 cut(s) 39, 236, 264, 454, 462, 514, 598, 638
TspRI CASTG 1 cut(s) 340
Tth111I GACNNNGTC 1 cut(s) 107
VneI GTGCAC 2 cut(s) 76, 458
VpaK11BI GGWCC 1 cut(s) 136
XapI RAATTY 1 cut(s) 682
XceI RCATGY 1 cut(s) 740
XmnI GAANNNNTTC 2 cut(s) 30, 92
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.