Rmu_sc0004279.1_g000004

Tobamovirus multiplication protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004279.1
OG0012412
Physical Location & Seq
Forward (+)
11172 .. 14656
3485 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 870 bp
atgccaccgccttcgctgctgacgtcggcgccgatggaggccgtggcgttcgtgatggcctggtgggacgatatcaacgagtcgacgaagtggcaggacggcatcttctattctctctgcgccgcctacgccctcgtctcctccgtcgctctgtttcaactaataaggattgaattgagagtgccggagtacggttggacgacgcagaaggtgtttcatcttatgaatttcattgtcaatggagtgcgagcaattgtctttggatttcacaagcaagtctttctcttgcaaccaagggtgttgatattggtcttattggatcttcctggactgctattcttctcaacgtacacacttcttgtcctattttgggcagagatatatcaccaggcgagaagtttaccgacagataagctcaggattgtttatatttcagtcaatggtggaatgtacttcgtacagatttgcctctggatatacctctggtttgatgacaacagcgttgtggagttcattggaaagatatttattgcagctgtgtcatttgtggctgcactaggtttcttaatttatggaggaaggttatttttcatgctgagacgtttcccaattgaatctaaagggagaaggaagaaacttcatgaggttggatctgttacagccatatgttttacctgctttctcataagatgctttgtggatgtgttgtctgcttttgatgatgatgcatcacttgatgttttggatcatcctattttgaatgcgttctactacatgctggttgagatcctgccctcagccttagtcctcttcatcttgcgcaaattaccccccaagagagtatcagctcagtatcatccaatacgttag

Protein Analysis

289

Amino Acids

33.24

Weight (kDa)

8.54

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 19 - 289 1.6e-141 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21790
fragaria_vesca FvH4_3g02360 FvH4_3g02360
malus_domestica MD05G1343500.v1.1 MD10G1318600.v1.1
prunus_persica Prupe.4G023400_v2.0.a1
pyrus_communis pycom05g31290
rosa_chinensis RchiOBHm_Chr5g0003581
rosa_laevigata RLG00000031154
rosa_multiflora Rmu_sc0004279.1_g000004 Rmu_sc0015897.1_g000002 Rmu_sc0022447.1_g000003
rosa_roxburghii Rroxscaffold_1G00072380
rosa_rugosa Rorug04G0404700 Rorug04G0404800
rosa_samantha Rh5AG031600 Rh5BG030500 Rh5CG034200
rosa_wichuraiana Rw5G002940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 26
Acc16I TGCGCA 1 cut(s) 821
Acc36I ACCTGC 1 cut(s) 683
AccB1I GGYRCC 1 cut(s) 28
AccI GTMKAC 1 cut(s) 83
AciI CCGC 2 cut(s) 8, 123
AclWI GGATC 4 cut(s) 327, 658, 753, 781
AcsI RAATTY 1 cut(s) 226
AcyI GRCGYC 2 cut(s) 23, 29
AfaI GTAC 4 cut(s) 191, 350, 452, 459
AfiI CCNNNNNNNGG 2 cut(s) 191, 370
AgsI TTSAA 4 cut(s) 158, 173, 614, 760
AjnI CCWGG 3 cut(s) 59, 325, 387
AluBI AGCT 3 cut(s) 415, 536, 848
AluI AGCT 3 cut(s) 415, 536, 848
Alw26I GTCTC 2 cut(s) 142, 592
AlwI GGATC 4 cut(s) 327, 658, 753, 781
AoxI GGCC 2 cut(s) 39, 57
ApeKI GCWGC 3 cut(s) 16, 533, 551
ApoI RAATTY 1 cut(s) 226
Asp700I GAANNNNTTC 1 cut(s) 764
AspLEI GCGC 3 cut(s) 31, 122, 822
AsuHPI GGTGA 1 cut(s) 377
BanI GGYRCC 1 cut(s) 28
BbvCI CCTCAGC 1 cut(s) 796
BbvI GCAGC 3 cut(s) 3, 538, 545
BccI CCATC 2 cut(s) 28, 49
BceAI ACGGC 2 cut(s) 26, 115
BciT130I CCWGG 3 cut(s) 61, 327, 389
BcoDI GTCTC 2 cut(s) 142, 592
BfaI CTAG 1 cut(s) 557
BfoI RGCGCY 1 cut(s) 32
BfuAI ACCTGC 1 cut(s) 683
BisI GCNGC 4 cut(s) 17, 123, 534, 552
BlsI GCNGC 4 cut(s) 18, 124, 535, 553
Bme1390I CCNGG 3 cut(s) 61, 327, 389
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 3 cut(s) 61, 327, 389
BmsI GCATC 4 cut(s) 111, 680, 715, 737
Bpu10I CCTNAGC 2 cut(s) 416, 796
BsaHI GRCGYC 2 cut(s) 23, 29
BsaJI CCNNGG 2 cut(s) 42, 293
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 370
BseBI CCWGG 3 cut(s) 61, 327, 389
BseDI CCNNGG 2 cut(s) 42, 293
BseGI GGATG 3 cut(s) 706, 748, 856
BseLI CCNNNNNNNGG 2 cut(s) 191, 370
BseMII CTCAG 4 cut(s) 430, 587, 810, 863
BseRI GAGGAG 1 cut(s) 130
BseXI GCAGC 3 cut(s) 3, 538, 545
BsgI GTGCAG 1 cut(s) 537
BshFI GGCC 2 cut(s) 41, 59
BshNI GGYRCC 1 cut(s) 28
BsiSI CCGG 1 cut(s) 185
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 2 cut(s) 191, 370
BsmAI GTCTC 2 cut(s) 142, 592
BsmBI CGTCTC 2 cut(s) 142, 592
BsmFI GGGAC 1 cut(s) 80
BsmI GAATGC 1 cut(s) 766
BsnI GGCC 2 cut(s) 41, 59
Bsp143I GATC 4 cut(s) 319, 650, 745, 786
BspACI CCGC 2 cut(s) 8, 123
BspANI GGCC 2 cut(s) 41, 59
BspCNI CTCAG 4 cut(s) 429, 588, 809, 862
BspHI TCATGA 1 cut(s) 640
BspLI GGNNCC 1 cut(s) 30
BspMI ACCTGC 1 cut(s) 683
BspPI GGATC 4 cut(s) 327, 658, 753, 781
BspT107I GGYRCC 1 cut(s) 28
BssECI CCNNGG 2 cut(s) 42, 293
BssMI GATC 4 cut(s) 319, 650, 745, 786
BssNI GRCGYC 2 cut(s) 23, 29
BssT1I CCWWGG 1 cut(s) 293
Bst2UI CCWGG 3 cut(s) 61, 327, 389
Bst4CI ACNGT 1 cut(s) 194
Bst6I CTCTTC 1 cut(s) 815
BstACI GRCGYC 2 cut(s) 23, 29
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 5 cut(s) 416, 596, 796, 802, 849
BstDSI CCRYGG 1 cut(s) 42
BstF5I GGATG 3 cut(s) 706, 748, 856
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 3 cut(s) 31, 122, 822
BstKTI GATC 4 cut(s) 322, 653, 748, 789
BstMAI GTCTC 2 cut(s) 142, 592
BstMBI GATC 4 cut(s) 319, 650, 745, 786
BstMWI GCNNNNNNNGC 2 cut(s) 16, 128
BstNI CCWGG 3 cut(s) 61, 327, 389
BstNSI RCATGY 1 cut(s) 778
BstSCI CCNGG 3 cut(s) 59, 325, 387
BstV1I GCAGC 3 cut(s) 3, 538, 545
BstX2I RGATCY 3 cut(s) 319, 650, 786
BstYI RGATCY 3 cut(s) 319, 650, 786
BsuRI GGCC 2 cut(s) 41, 59
BtgI CCRYGG 1 cut(s) 42
BtsCI GGATG 3 cut(s) 706, 748, 856
BveI ACCTGC 1 cut(s) 683
Cac8I GCNNGC 1 cut(s) 249
CciI TCATGA 1 cut(s) 640
CfoI GCGC 3 cut(s) 31, 122, 822
CseI GACGC 1 cut(s) 211
Csp6I GTAC 4 cut(s) 190, 349, 451, 458
CviAII CATG 3 cut(s) 592, 641, 775
CviJI RGCY 8 cut(s) 41, 59, 415, 536, 551, 662, 800, 848
CviKI_1 RGCY 8 cut(s) 41, 59, 415, 536, 551, 662, 800, 848
CviQI GTAC 4 cut(s) 190, 349, 451, 458
DdeI CTNAG 5 cut(s) 416, 596, 796, 802, 849
DinI GGCGCC 1 cut(s) 30
DpnI GATC 4 cut(s) 321, 652, 747, 788
DpnII GATC 4 cut(s) 319, 650, 745, 786
Eam1104I CTCTTC 1 cut(s) 815
EarI CTCTTC 1 cut(s) 815
Eco130I CCWWGG 1 cut(s) 293
Eco32I GATATC 1 cut(s) 73
EcoRII CCWGG 3 cut(s) 59, 325, 387
EcoRV GATATC 1 cut(s) 73
EcoT14I CCWWGG 1 cut(s) 293
EcoT22I ATGCAT 1 cut(s) 730
EgeI GGCGCC 1 cut(s) 30
EheI GGCGCC 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 293
Esp3I CGTCTC 2 cut(s) 142, 592
FaeI CATG 3 cut(s) 595, 644, 778
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FaqI GGGAC 1 cut(s) 80
FatI CATG 3 cut(s) 591, 640, 774
FauNDI CATATG 1 cut(s) 665
FblI GTMKAC 1 cut(s) 83
Fnu4HI GCNGC 4 cut(s) 17, 123, 534, 552
FokI GGATG 3 cut(s) 713, 735, 843
Fsp4HI GCNGC 4 cut(s) 17, 123, 534, 552
FspBI CTAG 1 cut(s) 557
FspI TGCGCA 1 cut(s) 821
GlaI GCGC 3 cut(s) 30, 121, 821
GluI GCNGC 4 cut(s) 17, 123, 534, 552
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 2 cut(s) 41, 59
HapII CCGG 1 cut(s) 185
HgaI GACGC 1 cut(s) 211
HhaI GCGC 3 cut(s) 31, 122, 822
Hin1I GRCGYC 2 cut(s) 23, 29
Hin1II CATG 3 cut(s) 595, 644, 778
Hin6I GCGC 3 cut(s) 29, 120, 820
HinP1I GCGC 3 cut(s) 29, 120, 820
HincII GTYRAC 1 cut(s) 84
HindII GTYRAC 1 cut(s) 84
HinfI GANTC 2 cut(s) 80, 614
HpaII CCGG 1 cut(s) 185
HphI GGTGA 1 cut(s) 377
Hpy166II GTNNAC 3 cut(s) 84, 351, 401
Hpy188III TCNNGA 4 cut(s) 52, 418, 472, 641
Hpy8I GTNNAC 3 cut(s) 84, 351, 401
Hpy99I CGWCG 4 cut(s) 28, 88, 149, 205
HpyAV CCTTC 4 cut(s) 21, 202, 573, 621
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4IV ACGT 4 cut(s) 23, 347, 601, 865
HpyCH4V TGCA 4 cut(s) 289, 533, 554, 728
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 128
HpyF3I CTNAG 5 cut(s) 416, 596, 796, 802, 849
HpySE526I ACGT 4 cut(s) 23, 347, 601, 865
Hsp92I GRCGYC 2 cut(s) 23, 29
Hsp92II CATG 3 cut(s) 595, 644, 778
HspAI GCGC 3 cut(s) 29, 120, 820
KasI GGCGCC 1 cut(s) 28
Kzo9I GATC 4 cut(s) 319, 650, 745, 786
Lsp1109I GCAGC 3 cut(s) 3, 538, 545
LweI GCATC 4 cut(s) 111, 680, 715, 737
MaeI CTAG 1 cut(s) 557
MaeII ACGT 4 cut(s) 23, 347, 601, 865
MaeIII GTNAC 1 cut(s) 655
MalI GATC 4 cut(s) 321, 652, 747, 788
MboI GATC 4 cut(s) 319, 650, 745, 786
MboII GAAGA 5 cut(s) 97, 314, 331, 643, 802
MfeI CAATTG 2 cut(s) 252, 609
MflI RGATCY 3 cut(s) 319, 650, 786
MluCI AATT 6 cut(s) 173, 226, 252, 567, 609, 824
Mly113I GGCGCC 1 cut(s) 29
MlyI GAGTC 1 cut(s) 89
MmeI TCCRAC 2 cut(s) 176, 628
MnlI CCTC 9 cut(s) 31, 143, 151, 479, 491, 569, 637, 805, 818
Mph1103I ATGCAT 1 cut(s) 730
MroXI GAANNNNTTC 1 cut(s) 764
MseI TTAA 1 cut(s) 566
MspA1I CMGCKG 1 cut(s) 536
MspI CCGG 1 cut(s) 185
MspR9I CCNGG 3 cut(s) 61, 327, 389
MunI CAATTG 2 cut(s) 252, 609
Mva1269I GAATGC 1 cut(s) 766
MvaI CCWGG 3 cut(s) 61, 327, 389
MwoI GCNNNNNNNGC 2 cut(s) 16, 128
NarI GGCGCC 1 cut(s) 29
NdeI CATATG 1 cut(s) 665
NdeII GATC 4 cut(s) 319, 650, 745, 786
NlaIII CATG 3 cut(s) 595, 644, 778
NlaIV GGNNCC 1 cut(s) 30
NsbI TGCGCA 1 cut(s) 821
NsiI ATGCAT 1 cut(s) 730
NspI RCATGY 1 cut(s) 778
PagI TCATGA 1 cut(s) 640
PcsI WCGNNNNNNNCGW 4 cut(s) 20, 29, 75, 141
PctI GAATGC 1 cut(s) 766
PdmI GAANNNNTTC 1 cut(s) 764
PfeI GAWTC 1 cut(s) 614
PfoI TCCNGGA 1 cut(s) 325
PkrI GCNGC 4 cut(s) 18, 124, 535, 553
PleI GAGTC 1 cut(s) 88
PluTI GGCGCC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 88
Psp6I CCWGG 3 cut(s) 59, 325, 387
PspGI CCWGG 3 cut(s) 59, 325, 387
PspN4I GGNNCC 1 cut(s) 30
PsuI RGATCY 3 cut(s) 319, 650, 786
PvuII CAGCTG 1 cut(s) 536
RsaI GTAC 4 cut(s) 191, 350, 452, 459
RsaNI GTAC 4 cut(s) 190, 349, 451, 458
SalI GTCGAC 1 cut(s) 82
SaqAI TTAA 1 cut(s) 566
SatI GCNGC 4 cut(s) 17, 123, 534, 552
Sau3AI GATC 4 cut(s) 319, 650, 745, 786
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 3 cut(s) 61, 327, 389
SfaNI GCATC 4 cut(s) 111, 680, 715, 737
SfoI GGCGCC 1 cut(s) 30
Sse9I AATT 6 cut(s) 173, 226, 252, 567, 609, 824
SsiI CCGC 2 cut(s) 8, 123
SspDI GGCGCC 1 cut(s) 28
SspMI CTAG 1 cut(s) 557
StyD4I CCNGG 3 cut(s) 59, 325, 387
StyI CCWWGG 1 cut(s) 293
TaaI ACNGT 1 cut(s) 194
TaiI ACGT 4 cut(s) 26, 350, 604, 868
TaqI TCGA 1 cut(s) 83
TasI AATT 6 cut(s) 173, 226, 252, 567, 609, 824
TatI WGTACW 1 cut(s) 450
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 1 cut(s) 614
Tru1I TTAA 1 cut(s) 566
Tru9I TTAA 1 cut(s) 566
TseI GCWGC 3 cut(s) 16, 533, 551
TspDTI ATGAA 7 cut(s) 206, 220, 239, 502, 580, 629, 802
TspGWI ACGGA 1 cut(s) 133
XapI RAATTY 1 cut(s) 226
XceI RCATGY 1 cut(s) 778
XmiI GTMKAC 1 cut(s) 83
XmnI GAANNNNTTC 1 cut(s) 764
XspI CTAG 1 cut(s) 557
ZraI GACGTC 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.