Rmu_sc0004517.1_g000004

One of the components of the core complex of photosystem II (PSII). It binds chlorophyll and helps catalyze the primary light-induced photochemical processes of PSII. PSII is a light- driven water plastoquinone oxidoreductase, using light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004517.1
OG0007092
Physical Location & Seq
Forward (+)
19215 .. 20177
963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004517.1_g000004.1.cds

Sequence Viewer

Length: 645 bp
atgggtttgccttggtatcgtgttcataccgtcgtattgaatgatcccggtcgtttgatttctgtccatataatgcatacagctctggttgctggttgggccggttcgatggctctatacgaattagccgtttttgatccctctgatcctgttcttgatccaatgtggagacagggggggactataacgaatcccggtatttggagttacgaaggtgtggctgaggcacatattgtgttttctggcttgtgttttttggcagctatctggcattgggtgtattgggatctagaaatatttactgatgaacgtactggaaaaccctctttggatttgcctaagatctttggaattcatttatttctctcagaggtggcttgctttgcttttggtgcatttcatgtaacaggattcctcctccgtccctcaggaccaacaaggggtagtacaggaatattcacctgttgtccatcgactacgcttttcggcctgatcttaggccctgactcaccctccgtggacgaaccttgcggaggaacccttaggttttcagggcattggattctcaccaatgtttgcgttactcaagccgacattctcgcttccgcttcgtccacccccgctcgcgcggttgcttccctctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
PSII

Protein Analysis

214

Amino Acids

23.17

Weight (kDa)

5.48

Isoelectric Point (pI)

29.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 623
AccII CGCG 2 cut(s) 627, 629
AciI CCGC 4 cut(s) 531, 606, 621, 629
AclWI GGATC 5 cut(s) 38, 131, 140, 152, 294
AcsI RAATTY 1 cut(s) 351
AfaI GTAC 2 cut(s) 313, 448
AfiI CCNNNNNNNGG 3 cut(s) 201, 440, 533
AgsI TTSAA 1 cut(s) 40
AluBI AGCT 2 cut(s) 83, 263
AluI AGCT 2 cut(s) 83, 263
Alw26I GTCTC 1 cut(s) 163
AlwI GGATC 5 cut(s) 38, 131, 140, 152, 294
AoxI GGCC 3 cut(s) 99, 487, 499
ApeKI GCWGC 1 cut(s) 260
ApoI RAATTY 1 cut(s) 351
AspLEI GCGC 1 cut(s) 629
AspS9I GGNCC 3 cut(s) 99, 431, 500
AsuC2I CCSGG 2 cut(s) 48, 195
AsuHPI GGTGA 3 cut(s) 451, 501, 559
AvaII GGWCC 1 cut(s) 431
AxyI CCTNAGG 2 cut(s) 427, 542
BbvCI CCTCAGC 1 cut(s) 222
BbvI GCAGC 1 cut(s) 272
BccI CCATC 2 cut(s) 103, 478
BceAI ACGGC 1 cut(s) 113
BcnI CCSGG 2 cut(s) 48, 195
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 2 cut(s) 290, 643
BglII AGATCT 1 cut(s) 342
BisI GCNGC 1 cut(s) 261
BlsI GCNGC 1 cut(s) 262
Bme1390I CCNGG 2 cut(s) 48, 195
Bme18I GGWCC 1 cut(s) 431
BmgT120I GGNCC 3 cut(s) 99, 431, 500
BmiI GGNNCC 1 cut(s) 538
BmrFI CCNGG 2 cut(s) 48, 195
Bpu10I CCTNAGC 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 570
BpuMI CCSGG 2 cut(s) 48, 195
BsaJI CCNNGG 2 cut(s) 11, 516
BsaXI ACNNNNNCTCC 2 cut(s) 497, 527
Bsc4I CCNNNNNNNGG 3 cut(s) 201, 440, 533
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 1 cut(s) 319
Bse21I CCTNAGG 2 cut(s) 427, 542
BseDI CCNNGG 2 cut(s) 11, 516
BseLI CCNNNNNNNGG 3 cut(s) 201, 440, 533
BseMII CTCAG 3 cut(s) 213, 381, 441
BseNI ACTGG 1 cut(s) 319
BseRI GAGGAG 1 cut(s) 407
BseXI GCAGC 1 cut(s) 272
Bsh1236I CGCG 2 cut(s) 627, 629
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 3 cut(s) 101, 489, 501
BsiEI CGRYCG 1 cut(s) 52
BsiSI CCGG 3 cut(s) 48, 102, 195
BslFI GGGAC 2 cut(s) 193, 408
BslI CCNNNNNNNGG 3 cut(s) 201, 440, 533
BsmAI GTCTC 1 cut(s) 163
BsmFI GGGAC 2 cut(s) 193, 408
BsnI GGCC 3 cut(s) 101, 489, 501
Bsp143I GATC 7 cut(s) 43, 136, 145, 157, 286, 342, 492
BspACI CCGC 4 cut(s) 531, 606, 621, 629
BspANI GGCC 3 cut(s) 101, 489, 501
BspCNI CTCAG 3 cut(s) 214, 380, 440
BspFNI CGCG 2 cut(s) 627, 629
BspLI GGNNCC 1 cut(s) 538
BspPI GGATC 5 cut(s) 38, 131, 140, 152, 294
BsrBI CCGCTC 1 cut(s) 623
BsrFI RCCGGY 1 cut(s) 101
BsrI ACTGG 1 cut(s) 319
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 2 cut(s) 11, 516
BssMI GATC 7 cut(s) 43, 136, 145, 157, 286, 342, 492
BssT1I CCWWGG 1 cut(s) 11
Bst4CI ACNGT 1 cut(s) 31
BstC8I GCNNGC 2 cut(s) 379, 625
BstDEI CTNAG 6 cut(s) 222, 339, 367, 427, 496, 542
BstDSI CCRYGG 1 cut(s) 516
BstENI CCTNNNNNAGG 1 cut(s) 531
BstFNI CGCG 2 cut(s) 627, 629
BstHHI GCGC 1 cut(s) 629
BstKTI GATC 7 cut(s) 46, 139, 148, 160, 289, 345, 495
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 7 cut(s) 43, 136, 145, 157, 286, 342, 492
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 4 cut(s) 89, 98, 383, 392
BstSCI CCNGG 2 cut(s) 46, 193
BstUI CGCG 2 cut(s) 627, 629
BstV1I GCAGC 1 cut(s) 272
BstX2I RGATCY 2 cut(s) 286, 342
BstYI RGATCY 2 cut(s) 286, 342
Bsu36I CCTNAGG 2 cut(s) 427, 542
BsuRI GGCC 3 cut(s) 101, 489, 501
BtgI CCRYGG 1 cut(s) 516
Cac8I GCNNGC 2 cut(s) 379, 625
CfoI GCGC 1 cut(s) 629
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 3 cut(s) 99, 431, 500
Csp6I GTAC 2 cut(s) 312, 447
CviAII CATG 1 cut(s) 401
CviQI GTAC 2 cut(s) 312, 447
DdeI CTNAG 6 cut(s) 222, 339, 367, 427, 496, 542
DpnI GATC 7 cut(s) 45, 138, 147, 159, 288, 344, 494
DpnII GATC 7 cut(s) 43, 136, 145, 157, 286, 342, 492
Eco130I CCWWGG 1 cut(s) 11
Eco47I GGWCC 1 cut(s) 431
Eco81I CCTNAGG 2 cut(s) 427, 542
EcoNI CCTNNNNNAGG 1 cut(s) 531
EcoO109I RGGNCCY 1 cut(s) 500
EcoRI GAATTC 1 cut(s) 351
EcoT14I CCWWGG 1 cut(s) 11
EcoT22I ATGCAT 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 11
FaeI CATG 1 cut(s) 404
FaiI YATR 8 cut(s) 27, 69, 71, 78, 118, 185, 231, 402
FaqI GGGAC 2 cut(s) 193, 408
FatI CATG 1 cut(s) 400
FauI CCCGC 1 cut(s) 628
Fnu4HI GCNGC 1 cut(s) 261
Fsp4HI GCNGC 1 cut(s) 261
FspBI CTAG 2 cut(s) 290, 643
GlaI GCGC 1 cut(s) 628
GluI GCNGC 1 cut(s) 261
HaeIII GGCC 3 cut(s) 101, 489, 501
HapII CCGG 3 cut(s) 48, 102, 195
HhaI GCGC 1 cut(s) 629
Hin1II CATG 1 cut(s) 404
Hin6I GCGC 1 cut(s) 627
HinP1I GCGC 1 cut(s) 627
HinfI GANTC 4 cut(s) 190, 411, 506, 562
HpaII CCGG 3 cut(s) 48, 102, 195
HphI GGTGA 3 cut(s) 451, 501, 559
Hpy166II GTNNAC 2 cut(s) 520, 615
Hpy188I TCNGA 2 cut(s) 145, 370
Hpy188III TCNNGA 3 cut(s) 155, 290, 429
Hpy8I GTNNAC 2 cut(s) 520, 615
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4IV ACGT 1 cut(s) 310
HpyCH4V TGCA 2 cut(s) 76, 395
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 98, 383, 392
HpyF3I CTNAG 6 cut(s) 222, 339, 367, 427, 496, 542
HpySE526I ACGT 1 cut(s) 310
Hsp92II CATG 1 cut(s) 404
HspAI GCGC 1 cut(s) 627
Kzo9I GATC 7 cut(s) 43, 136, 145, 157, 286, 342, 492
Lsp1109I GCAGC 1 cut(s) 272
MaeI CTAG 2 cut(s) 290, 643
MaeII ACGT 1 cut(s) 310
MaeIII GTNAC 3 cut(s) 206, 403, 580
MalI GATC 7 cut(s) 45, 138, 147, 159, 288, 344, 494
MbiI CCGCTC 1 cut(s) 623
MboI GATC 7 cut(s) 43, 136, 145, 157, 286, 342, 492
MflI RGATCY 2 cut(s) 286, 342
MluCI AATT 2 cut(s) 122, 351
MlyI GAGTC 1 cut(s) 500
MnlI CCTC 9 cut(s) 151, 217, 334, 364, 425, 428, 436, 523, 527
Mph1103I ATGCAT 1 cut(s) 78
MspI CCGG 3 cut(s) 48, 102, 195
MspR9I CCNGG 2 cut(s) 48, 195
MvnI CGCG 2 cut(s) 627, 629
MwoI GCNNNNNNNGC 4 cut(s) 89, 98, 383, 392
NciI CCSGG 2 cut(s) 48, 195
NdeII GATC 7 cut(s) 43, 136, 145, 157, 286, 342, 492
NlaIII CATG 1 cut(s) 404
NlaIV GGNNCC 1 cut(s) 538
NsiI ATGCAT 1 cut(s) 78
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 3 cut(s) 190, 411, 562
PkrI GCNGC 1 cut(s) 262
PleI GAGTC 1 cut(s) 500
PpsI GAGTC 1 cut(s) 500
PspN4I GGNNCC 1 cut(s) 538
PspPI GGNCC 3 cut(s) 99, 431, 500
PsuI RGATCY 2 cut(s) 286, 342
RsaI GTAC 2 cut(s) 313, 448
RsaNI GTAC 2 cut(s) 312, 447
SatI GCNGC 1 cut(s) 261
Sau3AI GATC 7 cut(s) 43, 136, 145, 157, 286, 342, 492
Sau96I GGNCC 3 cut(s) 99, 431, 500
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 2 cut(s) 48, 195
SetI ASST 8 cut(s) 85, 217, 265, 313, 375, 464, 529, 548
SinI GGWCC 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 585
SmoI CTYRAG 1 cut(s) 585
Sse9I AATT 2 cut(s) 122, 351
SsiI CCGC 4 cut(s) 531, 606, 621, 629
SspI AATATT 2 cut(s) 297, 456
SspMI CTAG 2 cut(s) 290, 643
StyD4I CCNGG 2 cut(s) 46, 193
StyI CCWWGG 1 cut(s) 11
TaaI ACNGT 1 cut(s) 31
TaiI ACGT 1 cut(s) 313
TaqI TCGA 2 cut(s) 107, 473
TasI AATT 2 cut(s) 122, 351
TatI WGTACW 1 cut(s) 446
TfiI GAWTC 3 cut(s) 190, 411, 562
TseI GCWGC 1 cut(s) 260
TspDTI ATGAA 4 cut(s) 14, 321, 344, 389
TspGWI ACGGA 2 cut(s) 410, 505
VpaK11BI GGWCC 1 cut(s) 431
XagI CCTNNNNNAGG 1 cut(s) 531
XapI RAATTY 1 cut(s) 351
XbaI TCTAGA 1 cut(s) 289
XspI CTAG 2 cut(s) 290, 643
Zsp2I ATGCAT 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.