Rmu_sc0004550.1_g000013

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004550.1
OG0013832
Physical Location & Seq
Forward (+)
37066 .. 37716
651 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 651 bp
atgttcacactactcttctctttggccaccttgataatcctaccgccatctccgatcagtgcctcctctccatctgtttctgctctcttcgctttcggcgattccaccgtggattctggcatgaacaacgacctcatgcctttcgttcggtgcgaccatcccccttacggcagagacctgccggaacacggccccaccggaaggtactccaatggaaagattgcaactgactttatggcctcaaacttgggactcaaggacctcttgcctgcttacctcgacccgaaactgaccgactatgatttgctcaccggggtcagtttcgcgtcgggaacttgcggcctagatgatctgaccttggagttgagccacgtctatagtatgggggatcagttggagtattttgatgaagccgtagtgaggatgaagataagtgttggggaaaagaagggtagagagattgtggaaaatgcactgtttgtggttagtgttggggcgaatgacttgatgctgaacgtgtatgagtttcctctaaccgagaggaaaactcagtttacgtctacagaccagtactctgattttctgctccagagacttgggtcctttattcaggtaatgaatttcatctctttgtacatatacatatcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

216

Amino Acids

23.87

Weight (kDa)

4.48

Isoelectric Point (pI)

23.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 29 - 184 4.2e-12 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40250
fragaria_vesca FvH4_7g24850 FvH4_7g24850
malus_domestica MD01G1156400.v1.1 MD07G1225100.v1.1
prunus_persica Prupe.2G253600_v2.0.a1
pyrus_communis pycom07g20380
rosa_chinensis RchiOBHm_Chr1g0370731
rosa_laevigata RLG00000027015
rosa_multiflora Rmu_co8466073.1_g000001 Rmu_sc0004550.1_g000013
rosa_rugosa Rorug01G0358700
rosa_samantha Rh1AG368400 Rh1BG331900 Rh1CG345800 Rh1DG363300
rosa_wichuraiana Rw1G032420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 186
AccI GTMKAC 1 cut(s) 560
AccII CGCG 1 cut(s) 326
AciI CCGC 2 cut(s) 44, 339
AclWI GGATC 1 cut(s) 396
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 1 cut(s) 619
AfaI GTAC 3 cut(s) 206, 572, 635
AfiI CCNNNNNNNGG 4 cut(s) 167, 188, 201, 420
AflIII ACRYGT 1 cut(s) 516
AjiI CACGTC 1 cut(s) 373
Alw26I GTCTC 2 cut(s) 168, 586
AlwI GGATC 1 cut(s) 396
AoxI GGCC 4 cut(s) 24, 190, 237, 340
ApoI RAATTY 1 cut(s) 619
AspS9I GGNCC 3 cut(s) 191, 259, 600
AsuC2I CCSGG 1 cut(s) 313
AsuHPI GGTGA 1 cut(s) 301
AvaII GGWCC 2 cut(s) 259, 600
BalI TGGCCA 1 cut(s) 26
BccI CCATC 3 cut(s) 55, 79, 165
BceAI ACGGC 3 cut(s) 184, 205, 398
BcnI CCSGG 1 cut(s) 313
BcoDI GTCTC 2 cut(s) 168, 586
BfaI CTAG 1 cut(s) 344
BfmI CTRYAG 2 cut(s) 376, 561
BfuAI ACCTGC 1 cut(s) 186
BisI GCNGC 1 cut(s) 340
BlsI GCNGC 1 cut(s) 341
BmcAI AGTACT 1 cut(s) 572
Bme1390I CCNGG 1 cut(s) 313
Bme18I GGWCC 2 cut(s) 259, 600
BmgBI CACGTC 1 cut(s) 373
BmgT120I GGNCC 3 cut(s) 191, 259, 600
BmiI GGNNCC 2 cut(s) 193, 601
BmrFI CCNGG 1 cut(s) 313
BmsI GCATC 1 cut(s) 498
BoxI GACNNNNGTC 1 cut(s) 598
BplI GAGNNNNNCTC 2 cut(s) 532, 564
BpmI CTGGAG 1 cut(s) 572
BpuEI CTTGAG 1 cut(s) 239
BpuMI CCSGG 1 cut(s) 313
BsaI GGTCTC 1 cut(s) 168
BsaJI CCNNGG 3 cut(s) 108, 312, 357
BsaWI WCCGGW 1 cut(s) 197
Bsc4I CCNNNNNNNGG 4 cut(s) 167, 188, 201, 420
Bse1I ACTGG 1 cut(s) 568
BseDI CCNNGG 3 cut(s) 108, 312, 357
BseGI GGATG 2 cut(s) 157, 429
BseLI CCNNNNNNNGG 4 cut(s) 167, 188, 201, 420
BseMII CTCAG 1 cut(s) 563
BseNI ACTGG 1 cut(s) 568
BseRI GAGGAG 1 cut(s) 55
Bsh1236I CGCG 1 cut(s) 326
BshFI GGCC 4 cut(s) 26, 192, 239, 342
BsiSI CCGG 3 cut(s) 182, 198, 312
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 4 cut(s) 167, 188, 201, 420
BsmAI GTCTC 2 cut(s) 168, 586
BsmFI GGGAC 1 cut(s) 264
BsnI GGCC 4 cut(s) 26, 192, 239, 342
Bso31I GGTCTC 1 cut(s) 168
Bsp1407I TGTACA 1 cut(s) 633
Bsp143I GATC 3 cut(s) 54, 349, 388
BspACI CCGC 2 cut(s) 44, 339
BspANI GGCC 4 cut(s) 26, 192, 239, 342
BspCNI CTCAG 1 cut(s) 562
BspFNI CGCG 1 cut(s) 326
BspLI GGNNCC 2 cut(s) 193, 601
BspMI ACCTGC 1 cut(s) 186
BspPI GGATC 1 cut(s) 396
BspTNI GGTCTC 1 cut(s) 168
BsrGI TGTACA 1 cut(s) 633
BsrI ACTGG 1 cut(s) 568
BssECI CCNNGG 3 cut(s) 108, 312, 357
BssMI GATC 3 cut(s) 54, 349, 388
BssT1I CCWWGG 1 cut(s) 357
Bst4CI ACNGT 2 cut(s) 109, 477
Bst6I CTCTTC 2 cut(s) 20, 92
BstAUI TGTACA 1 cut(s) 633
BstC8I GCNNGC 1 cut(s) 270
BstDEI CTNAG 1 cut(s) 549
BstDSI CCRYGG 1 cut(s) 108
BstF5I GGATG 2 cut(s) 157, 429
BstFNI CGCG 1 cut(s) 326
BstKTI GATC 3 cut(s) 57, 352, 391
BstMAI GTCTC 2 cut(s) 168, 586
BstMBI GATC 3 cut(s) 54, 349, 388
BstMWI GCNNNNNNNGC 1 cut(s) 89
BstPAI GACNNNNGTC 1 cut(s) 598
BstSCI CCNGG 1 cut(s) 311
BstSFI CTRYAG 2 cut(s) 376, 561
BstUI CGCG 1 cut(s) 326
BstXI CCANNNNNNTGG 1 cut(s) 596
BsuRI GGCC 4 cut(s) 26, 192, 239, 342
BtgI CCRYGG 1 cut(s) 108
BtrI CACGTC 1 cut(s) 373
BtsCI GGATG 2 cut(s) 157, 429
BtsIMutI CAGTG 2 cut(s) 64, 473
BveI ACCTGC 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 270
Cfr13I GGNCC 3 cut(s) 191, 259, 600
CseI GACGC 1 cut(s) 315
Csp6I GTAC 3 cut(s) 205, 571, 634
CviAII CATG 2 cut(s) 121, 136
CviJI RGCY 6 cut(s) 26, 192, 239, 342, 369, 413
CviKI_1 RGCY 6 cut(s) 26, 192, 239, 342, 369, 413
CviQI GTAC 3 cut(s) 205, 571, 634
DdeI CTNAG 1 cut(s) 549
DpnI GATC 3 cut(s) 56, 351, 390
DpnII GATC 3 cut(s) 54, 349, 388
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 2 cut(s) 20, 92
EarI CTCTTC 2 cut(s) 20, 92
Eco130I CCWWGG 1 cut(s) 357
Eco31I GGTCTC 1 cut(s) 168
Eco47I GGWCC 2 cut(s) 259, 600
EcoO109I RGGNCCY 2 cut(s) 259, 600
EcoT14I CCWWGG 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 2 cut(s) 124, 139
FalI AAGNNNNNCTT 2 cut(s) 248, 280
FaqI GGGAC 1 cut(s) 264
FatI CATG 2 cut(s) 120, 135
FblI GTMKAC 1 cut(s) 560
Fnu4HI GCNGC 1 cut(s) 340
FokI GGATG 2 cut(s) 144, 436
Fsp4HI GCNGC 1 cut(s) 340
FspBI CTAG 1 cut(s) 344
GluI GCNGC 1 cut(s) 340
GsuI CTGGAG 1 cut(s) 572
HaeIII GGCC 4 cut(s) 26, 192, 239, 342
HapII CCGG 3 cut(s) 182, 198, 312
HgaI GACGC 1 cut(s) 315
Hin1II CATG 2 cut(s) 124, 139
HinfI GANTC 3 cut(s) 101, 113, 252
HpaII CCGG 3 cut(s) 182, 198, 312
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 3 cut(s) 6, 555, 561
Hpy188I TCNGA 3 cut(s) 54, 354, 577
Hpy188III TCNNGA 3 cut(s) 330, 589, 648
Hpy8I GTNNAC 3 cut(s) 6, 555, 561
Hpy99I CGWCG 1 cut(s) 331
HpyAV CCTTC 2 cut(s) 195, 442
HpyCH4III ACNGT 2 cut(s) 109, 477
HpyCH4IV ACGT 3 cut(s) 372, 516, 557
HpyCH4V TGCA 2 cut(s) 224, 473
HpyF10VI GCNNNNNNNGC 1 cut(s) 89
HpyF3I CTNAG 1 cut(s) 549
HpySE526I ACGT 3 cut(s) 372, 516, 557
Hsp92II CATG 2 cut(s) 124, 139
Kzo9I GATC 3 cut(s) 54, 349, 388
LmnI GCTCC 1 cut(s) 591
LpnPI CCDG 9 cut(s) 102, 191, 195, 211, 282, 325, 581, 596, 602
LweI GCATC 1 cut(s) 498
MaeI CTAG 1 cut(s) 344
MaeII ACGT 3 cut(s) 372, 516, 557
MalI GATC 3 cut(s) 56, 351, 390
MboI GATC 3 cut(s) 54, 349, 388
MboII GAAGA 3 cut(s) 7, 79, 439
MlsI TGGCCA 1 cut(s) 26
MluCI AATT 1 cut(s) 619
MluNI TGGCCA 1 cut(s) 26
MlyI GAGTC 1 cut(s) 246
MmeI TCCRAC 1 cut(s) 375
MnlI CCTC 9 cut(s) 73, 76, 143, 250, 272, 287, 414, 534, 540
Mox20I TGGCCA 1 cut(s) 26
MscI TGGCCA 1 cut(s) 26
Msp20I TGGCCA 1 cut(s) 26
MspI CCGG 3 cut(s) 182, 198, 312
MspR9I CCNGG 1 cut(s) 313
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 1 cut(s) 89
NciI CCSGG 1 cut(s) 313
NdeII GATC 3 cut(s) 54, 349, 388
NlaIII CATG 2 cut(s) 124, 139
NlaIV GGNNCC 2 cut(s) 193, 601
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 150
PfeI GAWTC 2 cut(s) 101, 113
PkrI GCNGC 1 cut(s) 341
PleI GAGTC 1 cut(s) 246
PpsI GAGTC 1 cut(s) 246
PpuMI RGGWCCY 2 cut(s) 259, 600
PshAI GACNNNNGTC 1 cut(s) 598
Psp5II RGGWCCY 2 cut(s) 259, 600
PspN4I GGNNCC 2 cut(s) 193, 601
PspPI GGNCC 3 cut(s) 191, 259, 600
PspPPI RGGWCCY 2 cut(s) 259, 600
RsaI GTAC 3 cut(s) 206, 572, 635
RsaNI GTAC 3 cut(s) 205, 571, 634
SatI GCNGC 1 cut(s) 340
Sau3AI GATC 3 cut(s) 54, 349, 388
Sau96I GGNCC 3 cut(s) 191, 259, 600
ScaI AGTACT 1 cut(s) 572
SchI GAGTC 1 cut(s) 246
ScrFI CCNGG 1 cut(s) 313
SfaNI GCATC 1 cut(s) 498
SfcI CTRYAG 2 cut(s) 376, 561
SinI GGWCC 2 cut(s) 259, 600
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 1 cut(s) 619
SsiI CCGC 2 cut(s) 44, 339
SspMI CTAG 1 cut(s) 344
StyD4I CCNGG 1 cut(s) 311
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 2 cut(s) 109, 477
TaiI ACGT 3 cut(s) 375, 519, 560
TaqI TCGA 1 cut(s) 279
TaqII GACCGA 1 cut(s) 308
TasI AATT 1 cut(s) 619
TatI WGTACW 2 cut(s) 570, 633
TauI GCSGC 1 cut(s) 342
TfiI GAWTC 2 cut(s) 101, 113
TscAI CASTG 2 cut(s) 64, 480
TspDTI ATGAA 5 cut(s) 137, 423, 440, 613, 632
TspRI CASTG 2 cut(s) 64, 480
VpaK11BI GGWCC 2 cut(s) 259, 600
XapI RAATTY 1 cut(s) 619
XmiI GTMKAC 1 cut(s) 560
XspI CTAG 1 cut(s) 344
ZrmI AGTACT 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.