Rmu_sc0004722.1_g000002

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004722.1
OG0006747
Physical Location & Seq
Forward (+)
7128 .. 11299
4172 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1338 bp
atgtctgcatctccccttgacgactacggtctttggagttctgataacttccgcaaacgacggggttttgcgccgaagctgttgattgtgttggtgaagatggaccctgcgttcgcggatgagggccacgacggtgagaccgtgacaatcgaagagtatctgaaggatgttgaggaccaggaactggaagctgatttggttttgggtggcgatgaaggcaaggagtgtacctataataatgggtacatgaagaggcaagcaattttctcatgcttgacttgcacaccagatggtaatgctggagtttgcacagcttgcagcttgtcttgtcatgatggccatgagattgtggaactatggaccaagagaaacttcagatgtgattgtggaaattcaaaatttggtgagttctactgcaagattttcccaagtaaagatattgaaaatgttgagaactcctacaatcataacttcaaaggttcctactgtacctgtggccgcccctatcctgatccagacgtggaagaacaagtagagatgatacagtgctgcttctgtgaggactggttccatgaggaacatattggtcttgattcctctgatgagattccaagagatggagaaggtgaacctctatatgaggattttatatgcgcagcatgctcaaaagtgtgctcctttctaagactatatactcgaatcatcttggaagcaaggagccaaaacaattcttctgttaagactggaaaagataaagaagttatagcagatgaagtacctttagcttgtggatctggaaagctagaaaatattcctcagagttctactaccaactctgaatctgtatctggaggggaagtttcacttggagaaaattctgggaagaatatatgttcagagcaatgtgctacagatgctgatacacgtcctacttgcgctattggagttgatctggcagttgcttcccctgtttcagagagtaagccaatgtttctttcaaaaaactggcggaatgctctttgcaggtgtgaaaagtgcagtgaattttataaacagaagcatattaattttctaattgacaaggaggactcaatcattgagtatgagaaaatggcaaagcagaagagagaagaaaagttgcagcaacgtgagggggcagagttaagtatgctggataaccttggccatgtagagaaaattgagattctgaatggtattgcagatatcaaagatgagctttgttctttcctgggatcatttgatccatcgaaagcaatcacatctgctgatgtccaccagatttttgagaatctaaagaaacgcaggcgtgtagagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBR

Protein Analysis

445

Amino Acids

49.87

Weight (kDa)

4.78

Isoelectric Point (pI)

55.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBR PF02207 76 - 140 2.5e-13 Putative zinc finger in N-recognin (UBR box)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1050
AarI CACCTGC 1 cut(s) 1014
Acc16I TGCGCA 1 cut(s) 655
Acc36I ACCTGC 1 cut(s) 1014
AccB7I CCANNNNNTGG 2 cut(s) 184, 617
AccII CGCG 1 cut(s) 116
AciI CCGC 4 cut(s) 52, 116, 499, 1009
AclWI GGATC 4 cut(s) 506, 799, 1256, 1261
AcoI YGGCCR 3 cut(s) 337, 496, 1183
AcsI RAATTY 4 cut(s) 391, 398, 874, 1043
AcuI CTGAAG 2 cut(s) 182, 358
AfaI GTAC 4 cut(s) 229, 245, 490, 777
AfiI CCNNNNNNNGG 2 cut(s) 184, 617
AflIII ACRYGT 1 cut(s) 923
AgsI TTSAA 4 cut(s) 396, 443, 475, 999
AjiI CACGTC 2 cut(s) 520, 926
AjnI CCWGG 2 cut(s) 177, 1248
AjuI GAANNNNNNNTTGG 6 cut(s) 356, 388, 849, 881, 979, 1011
AleI CACNNNNGTG 1 cut(s) 132
AloI GAACNNNNNNTCC 2 cut(s) 95, 127
AluBI AGCT 7 cut(s) 79, 191, 314, 321, 785, 802, 1237
AluI AGCT 7 cut(s) 79, 191, 314, 321, 785, 802, 1237
Alw21I GWGCWC 1 cut(s) 677
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 4 cut(s) 506, 799, 1256, 1261
AlwNI CAGNNNCTG 2 cut(s) 184, 917
AoxI GGCC 4 cut(s) 124, 337, 496, 1183
ApeKI GCWGC 4 cut(s) 318, 549, 656, 1141
ApoI RAATTY 4 cut(s) 391, 398, 874, 1043
ArsI GACNNNNNNTTYG 2 cut(s) 95, 127
AseI ATTAAT 1 cut(s) 1065
Asp700I GAANNNNTTC 1 cut(s) 810
AspLEI GCGC 3 cut(s) 73, 656, 938
AspS9I GGNCC 4 cut(s) 103, 124, 175, 360
AsuHPI GGTGA 4 cut(s) 106, 146, 416, 638
AvaII GGWCC 3 cut(s) 103, 175, 360
BalI TGGCCA 2 cut(s) 339, 1185
Bbv12I GWGCWC 1 cut(s) 677
BbvI GCAGC 4 cut(s) 330, 536, 668, 1153
BccI CCATC 5 cut(s) 94, 284, 329, 611, 1273
BciT130I CCWGG 2 cut(s) 179, 1250
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 1 cut(s) 803
BfmI CTRYAG 1 cut(s) 909
BfuAI ACCTGC 1 cut(s) 1014
BisI GCNGC 5 cut(s) 319, 499, 550, 657, 1142
BlsI GCNGC 5 cut(s) 320, 500, 551, 658, 1143
Bme1390I CCNGG 2 cut(s) 179, 1250
Bme18I GGWCC 3 cut(s) 103, 175, 360
BmgBI CACGTC 2 cut(s) 520, 926
BmgT120I GGNCC 4 cut(s) 103, 124, 175, 360
BmiI GGNNCC 4 cut(s) 105, 481, 569, 719
BmrFI CCNGG 2 cut(s) 179, 1250
BmsI GCATC 2 cut(s) 17, 904
BoxI GACNNNNGTC 1 cut(s) 27
BpmI CTGGAG 2 cut(s) 321, 870
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 2 cut(s) 1180, 1249
BsaXI ACNNNNNCTCC 2 cut(s) 843, 873
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 617
Bse1I ACTGG 4 cut(s) 189, 569, 748, 1010
Bse3DI GCAATG 1 cut(s) 908
BseBI CCWGG 2 cut(s) 179, 1250
BseDI CCNNGG 2 cut(s) 1180, 1249
BseGI GGATG 2 cut(s) 124, 172
BseLI CCNNNNNNNGG 2 cut(s) 184, 617
BseMI GCAATG 1 cut(s) 908
BseMII CTCAG 1 cut(s) 830
BseNI ACTGG 4 cut(s) 189, 569, 748, 1010
BseXI GCAGC 4 cut(s) 330, 536, 668, 1153
BsgI GTGCAG 1 cut(s) 1057
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 4 cut(s) 126, 339, 498, 1185
BsiHKAI GWGCWC 1 cut(s) 677
BslI CCNNNNNNNGG 2 cut(s) 184, 617
BsmAI GTCTC 1 cut(s) 131
BsmI GAATGC 1 cut(s) 1018
BsnI GGCC 4 cut(s) 126, 339, 498, 1185
Bso31I GGTCTC 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 677
Bsp143I GATC 5 cut(s) 511, 791, 949, 1253, 1261
BspACI CCGC 4 cut(s) 52, 116, 499, 1009
BspANI GGCC 4 cut(s) 126, 339, 498, 1185
BspCNI CTCAG 1 cut(s) 829
BspFNI CGCG 1 cut(s) 116
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 4 cut(s) 105, 481, 569, 719
BspMI ACCTGC 1 cut(s) 1014
BspPI GGATC 4 cut(s) 506, 799, 1256, 1261
BspTNI GGTCTC 1 cut(s) 131
BsrDI GCAATG 1 cut(s) 908
BsrI ACTGG 4 cut(s) 189, 569, 748, 1010
BssECI CCNNGG 2 cut(s) 1180, 1249
BssMI GATC 5 cut(s) 511, 791, 949, 1253, 1261
BssT1I CCWWGG 1 cut(s) 1180
Bst2UI CCWGG 2 cut(s) 179, 1250
Bst4CI ACNGT 5 cut(s) 29, 134, 142, 488, 546
Bst6I CTCTTC 3 cut(s) 147, 245, 1118
BstAPI GCANNNNNTGC 1 cut(s) 315
BstC8I GCNNGC 4 cut(s) 258, 316, 661, 1325
BstDEI CTNAG 2 cut(s) 683, 816
BstF5I GGATG 2 cut(s) 124, 172
BstFNI CGCG 1 cut(s) 116
BstHHI GCGC 3 cut(s) 73, 656, 938
BstKTI GATC 5 cut(s) 514, 794, 952, 1256, 1264
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 5 cut(s) 511, 791, 949, 1253, 1261
BstMWI GCNNNNNNNGC 5 cut(s) 216, 279, 315, 660, 914
BstNI CCWGG 2 cut(s) 179, 1250
BstNSI RCATGY 1 cut(s) 663
BstPAI GACNNNNGTC 1 cut(s) 27
BstSCI CCNGG 2 cut(s) 177, 1248
BstSFI CTRYAG 1 cut(s) 909
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 4 cut(s) 330, 536, 668, 1153
BstX2I RGATCY 1 cut(s) 791
BstYI RGATCY 1 cut(s) 791
BsuRI GGCC 4 cut(s) 126, 339, 498, 1185
BtgZI GCGATG 1 cut(s) 225
BtrI CACGTC 2 cut(s) 520, 926
BtsCI GGATG 2 cut(s) 124, 172
BtsI GCAGTG 1 cut(s) 1045
BtsIMutI CAGTG 2 cut(s) 551, 1045
BveI ACCTGC 1 cut(s) 1014
Cac8I GCNNGC 4 cut(s) 258, 316, 661, 1325
CaiI CAGNNNCTG 2 cut(s) 184, 917
CciI TCATGA 1 cut(s) 331
CfoI GCGC 3 cut(s) 73, 656, 938
Cfr13I GGNCC 4 cut(s) 103, 124, 175, 360
Csp6I GTAC 4 cut(s) 228, 244, 489, 776
CviAII CATG 7 cut(s) 247, 270, 332, 341, 572, 660, 1187
CviQI GTAC 4 cut(s) 228, 244, 489, 776
DdeI CTNAG 2 cut(s) 683, 816
DpnI GATC 5 cut(s) 513, 793, 951, 1255, 1263
DpnII GATC 5 cut(s) 511, 791, 949, 1253, 1261
EaeI YGGCCR 3 cut(s) 337, 496, 1183
Eam1104I CTCTTC 3 cut(s) 147, 245, 1118
EarI CTCTTC 3 cut(s) 147, 245, 1118
EciI GGCGGA 1 cut(s) 1024
Eco130I CCWWGG 1 cut(s) 1180
Eco31I GGTCTC 1 cut(s) 131
Eco32I GATATC 1 cut(s) 1225
Eco47I GGWCC 3 cut(s) 103, 175, 360
Eco57I CTGAAG 2 cut(s) 182, 358
EcoRII CCWGG 2 cut(s) 177, 1248
EcoRV GATATC 1 cut(s) 1225
EcoT14I CCWWGG 1 cut(s) 1180
ErhI CCWWGG 1 cut(s) 1180
FaeI CATG 7 cut(s) 250, 273, 335, 344, 575, 663, 1190
FalI AAGNNNNNCTT 6 cut(s) 356, 388, 849, 881, 1221, 1253
FatI CATG 7 cut(s) 246, 269, 331, 340, 571, 659, 1186
Fnu4HI GCNGC 5 cut(s) 319, 499, 550, 657, 1142
FokI GGATG 2 cut(s) 131, 179
Fsp4HI GCNGC 5 cut(s) 319, 499, 550, 657, 1142
FspBI CTAG 1 cut(s) 803
FspI TGCGCA 1 cut(s) 655
GlaI GCGC 3 cut(s) 72, 655, 937
GluI GCNGC 5 cut(s) 319, 499, 550, 657, 1142
GsuI CTGGAG 2 cut(s) 321, 870
HaeIII GGCC 4 cut(s) 126, 339, 498, 1185
HhaI GCGC 3 cut(s) 73, 656, 938
Hin1II CATG 7 cut(s) 250, 273, 335, 344, 575, 663, 1190
Hin6I GCGC 3 cut(s) 71, 654, 936
HinP1I GCGC 3 cut(s) 71, 654, 936
HinfI GANTC 7 cut(s) 593, 607, 699, 839, 1088, 1204, 1309
HphI GGTGA 4 cut(s) 106, 146, 416, 638
Hpy166II GTNNAC 3 cut(s) 228, 629, 1294
Hpy188I TCNGA 9 cut(s) 43, 162, 377, 601, 819, 838, 898, 976, 1209
Hpy188III TCNNGA 6 cut(s) 332, 509, 515, 590, 795, 849
Hpy8I GTNNAC 3 cut(s) 228, 629, 1294
Hpy99I CGWCG 2 cut(s) 63, 134
HpyAV CCTTC 3 cut(s) 157, 209, 617
HpyCH4III ACNGT 5 cut(s) 29, 134, 142, 488, 546
HpyCH4IV ACGT 3 cut(s) 519, 925, 1147
HpyCH4V TGCA 9 cut(s) 8, 282, 309, 318, 417, 1023, 1038, 1141, 1220
HpyF10VI GCNNNNNNNGC 5 cut(s) 216, 279, 315, 660, 914
HpyF3I CTNAG 2 cut(s) 683, 816
HpySE526I ACGT 3 cut(s) 519, 925, 1147
Hsp92II CATG 7 cut(s) 250, 273, 335, 344, 575, 663, 1190
HspAI GCGC 3 cut(s) 71, 654, 936
Kzo9I GATC 5 cut(s) 511, 791, 949, 1253, 1261
LmnI GCTCC 2 cut(s) 680, 717
Lsp1109I GCAGC 4 cut(s) 330, 536, 668, 1153
LweI GCATC 2 cut(s) 17, 904
MaeI CTAG 1 cut(s) 803
MaeII ACGT 3 cut(s) 519, 925, 1147
MaeIII GTNAC 1 cut(s) 142
MalI GATC 5 cut(s) 513, 793, 951, 1255, 1263
MboI GATC 5 cut(s) 511, 791, 949, 1253, 1261
MboII GAAGA 8 cut(s) 109, 164, 262, 536, 723, 895, 1135, 1142
MflI RGATCY 1 cut(s) 791
MhlI GDGCHC 1 cut(s) 677
MlsI TGGCCA 2 cut(s) 339, 1185
MluCI AATT 9 cut(s) 261, 391, 398, 727, 874, 1043, 1066, 1074, 1197
MluNI TGGCCA 2 cut(s) 339, 1185
MlyI GAGTC 1 cut(s) 1082
Mox20I TGGCCA 2 cut(s) 339, 1185
MroXI GAANNNNTTC 1 cut(s) 810
MscI TGGCCA 2 cut(s) 339, 1185
MseI TTAA 3 cut(s) 738, 1065, 1163
MslI CAYNNNNRTG 1 cut(s) 132
Msp20I TGGCCA 2 cut(s) 339, 1185
MspR9I CCNGG 2 cut(s) 179, 1250
Mva1269I GAATGC 1 cut(s) 1018
MvaI CCWGG 2 cut(s) 179, 1250
MvnI CGCG 1 cut(s) 116
MwoI GCNNNNNNNGC 5 cut(s) 216, 279, 315, 660, 914
NdeII GATC 5 cut(s) 511, 791, 949, 1253, 1261
NlaIII CATG 7 cut(s) 250, 273, 335, 344, 575, 663, 1190
NlaIV GGNNCC 4 cut(s) 105, 481, 569, 719
NmuCI GTSAC 1 cut(s) 142
NsbI TGCGCA 1 cut(s) 655
NspI RCATGY 1 cut(s) 663
OliI CACNNNNGTG 1 cut(s) 132
PaeI GCATGC 1 cut(s) 663
PagI TCATGA 1 cut(s) 331
PaqCI CACCTGC 1 cut(s) 1014
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PctI GAATGC 1 cut(s) 1018
PdmI GAANNNNTTC 1 cut(s) 810
PfeI GAWTC 6 cut(s) 593, 607, 699, 839, 1204, 1309
PflMI CCANNNNNTGG 2 cut(s) 184, 617
PkrI GCNGC 5 cut(s) 320, 500, 551, 658, 1143
PleI GAGTC 1 cut(s) 1082
PpsI GAGTC 1 cut(s) 1082
PshAI GACNNNNGTC 1 cut(s) 27
PshBI ATTAAT 1 cut(s) 1065
PsiI TTATAA 1 cut(s) 1050
Psp6I CCWGG 2 cut(s) 177, 1248
PspGI CCWGG 2 cut(s) 177, 1248
PspN4I GGNNCC 4 cut(s) 105, 481, 569, 719
PspPI GGNCC 4 cut(s) 103, 124, 175, 360
PstNI CAGNNNCTG 2 cut(s) 184, 917
PsuI RGATCY 1 cut(s) 791
RsaI GTAC 4 cut(s) 229, 245, 490, 777
RsaNI GTAC 4 cut(s) 228, 244, 489, 776
RseI CAYNNNNRTG 1 cut(s) 132
SaqAI TTAA 3 cut(s) 738, 1065, 1163
SatI GCNGC 5 cut(s) 319, 499, 550, 657, 1142
Sau3AI GATC 5 cut(s) 511, 791, 949, 1253, 1261
Sau96I GGNCC 4 cut(s) 103, 124, 175, 360
SchI GAGTC 1 cut(s) 1082
ScrFI CCNGG 2 cut(s) 179, 1250
SduI GDGCHC 1 cut(s) 677
SfaNI GCATC 2 cut(s) 17, 904
SfcI CTRYAG 1 cut(s) 909
SinI GGWCC 3 cut(s) 103, 175, 360
SmiMI CAYNNNNRTG 1 cut(s) 132
SphI GCATGC 1 cut(s) 663
Sse9I AATT 9 cut(s) 261, 391, 398, 727, 874, 1043, 1066, 1074, 1197
SsiI CCGC 4 cut(s) 52, 116, 499, 1009
SspI AATATT 1 cut(s) 811
SspMI CTAG 1 cut(s) 803
StyD4I CCNGG 2 cut(s) 177, 1248
StyI CCWWGG 1 cut(s) 1180
TaaI ACNGT 5 cut(s) 29, 134, 142, 488, 546
TaiI ACGT 3 cut(s) 522, 928, 1150
TaqI TCGA 3 cut(s) 150, 697, 1268
TasI AATT 9 cut(s) 261, 391, 398, 727, 874, 1043, 1066, 1074, 1197
TauI GCSGC 1 cut(s) 501
TfiI GAWTC 6 cut(s) 593, 607, 699, 839, 1204, 1309
Tru1I TTAA 3 cut(s) 738, 1065, 1163
Tru9I TTAA 3 cut(s) 738, 1065, 1163
TscAI CASTG 2 cut(s) 551, 1045
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 4 cut(s) 318, 549, 656, 1141
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 3 cut(s) 228, 263, 786
TspRI CASTG 2 cut(s) 551, 1045
Van91I CCANNNNNTGG 2 cut(s) 184, 617
VpaK11BI GGWCC 3 cut(s) 103, 175, 360
VspI ATTAAT 1 cut(s) 1065
XapI RAATTY 4 cut(s) 391, 398, 874, 1043
XceI RCATGY 1 cut(s) 663
XmnI GAANNNNTTC 1 cut(s) 810
XspI CTAG 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.