Rmu_sc0004889.1_g000024

Phospholipase A1-Igamma1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004889.1
OG0009590
Physical Location & Seq
Forward (+)
96822 .. 98384
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1563 bp
atgatgtatgctcatattccggccactcaagcggcctccgcagcagcccggtttcccttgatccgatctcaacagcaccaagaagctgttcttgatcatcacccacaaacaaagacatcaaacaggaccaaccgtccagctgaatcgctagcccacctcctccatcttcacatagaaacccctcctaaaaccgacagtcctcgcttttccaattggaatatgtttactgaagagaagcattcaactcccacaacttctcccaaggaaataatatccgaaaagtggcgtgaaatccacggctcaaaagattgggagggtctaatggatccactccacccttggctaagaagagagatagtcaagtacggtgaatttgcacaggcaacatatgatgctttcgactttgactcattctctgagttctgcgggagctgcaggtacaacaagaacaagctctttgatgttttgggactgagcaaaaatggatatgcagttagcaaatacatttacgccatgtcccatatagacatgcctaattggctggagaggtcacatttggttgagacttggagcaaagattcaaactggatgggatttgttgcagtgagtgatgacgaggagacgagaaggatcggaaggagagatattgctgtggcttggcgtgggacggtggcgccatccgaatggtatgaggatctgcagaggaagttggaacctattggagatttggatgccaaagttgaacatgggtttcttgggatttacaagtccaagagtgagaaaaccaggtacaacaaatctagtgcctcagagcaagtcatgaaggaagtgactaggcttgttgggttttataaatccaagggtgaagaagtgagtcttacaattacagggcatagcttgggtggtgctttggctttgctcaatgcttacgaagctgcaaaatcaattcctgggctacccattagtgtcatttcatttggggcacctagagtgggaaacaatgctttcagagatgagctaaaccaaatgggggtcaagacactgagggttgtggtcaagcaagacgtggttccgaaaatgccgggacttgttttgaatgaaggtttgcagaagtttgaagaaattaccggcacattggaatgggtttatacacatgttggagctgaactgaaagttgatgtcggctcgtcgccttatctcaagaaacatggtttcaattttctagggtttcatagccttgagacttatcttcaccttgtggatgggtatttgaataaagaaactagctttaggtcaaatgccaggagggattttgccttggtgaacaaggcatgtgacatgttggtggaggatcttagaatcccacactgttggtaccaattggaacacaagggacttgtttgtaatcatcatgggagatgggttaaacccataagagagcctgaagacattccttcacctactagagaagcagaagaacaagctcatgctcttcaagtagagatacaatcagattatgtacttcaatctttatgtaccgctaatatgaaaggtgaattttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

520

Amino Acids

59.22

Weight (kDa)

6.46

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 218 - 363 9.7e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 852
Acc36I ACCTGC 1 cut(s) 426
Acc65I GGTACC 1 cut(s) 1374
AccB1I GGYRCC 3 cut(s) 673, 982, 1374
AciI CCGC 4 cut(s) 32, 39, 426, 1539
AclWI GGATC 6 cut(s) 55, 320, 333, 638, 702, 1359
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 2 cut(s) 371, 1556
AcuI CTGAAG 2 cut(s) 249, 1464
AcyI GRCGYC 1 cut(s) 674
AdeI CACNNNGTG 1 cut(s) 1258
AfaI GTAC 6 cut(s) 365, 440, 791, 1376, 1521, 1537
AfiI CCNNNNNNNGG 3 cut(s) 282, 992, 1030
AflIII ACRYGT 2 cut(s) 1153, 1338
AgsI TTSAA 9 cut(s) 243, 582, 743, 1096, 1118, 1216, 1273, 1496, 1526
AhdI GACNNNNNGTC 1 cut(s) 132
AjiI CACGTC 1 cut(s) 1066
AjnI CCWGG 3 cut(s) 785, 949, 1301
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
Alw26I GTCTC 3 cut(s) 557, 614, 1235
AlwI GGATC 6 cut(s) 55, 320, 333, 638, 702, 1359
AoxI GGCC 2 cut(s) 21, 33
ApeKI GCWGC 4 cut(s) 41, 44, 432, 935
ApoI RAATTY 2 cut(s) 371, 1556
Asp700I GAANNNNTTC 2 cut(s) 87, 1449
Asp718I GGTACC 1 cut(s) 1374
AspLEI GCGC 1 cut(s) 676
AspS9I GGNCC 1 cut(s) 126
AsuC2I CCSGG 2 cut(s) 49, 1083
AsuHPI GGTGA 6 cut(s) 92, 380, 875, 1244, 1333, 1449
AsuNHI GCTAGC 1 cut(s) 148
AvaII GGWCC 1 cut(s) 126
BaeGI GKGCMC 1 cut(s) 985
BamHI GGATCC 1 cut(s) 325
BanI GGYRCC 3 cut(s) 673, 982, 1374
BbsI GAAGAC 1 cut(s) 1452
BbvI GCAGC 4 cut(s) 53, 56, 419, 922
BccI CCATC 5 cut(s) 171, 583, 685, 1256, 1413
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 3 cut(s) 787, 951, 1303
BclI TGATCA 1 cut(s) 94
BcnI CCSGG 2 cut(s) 49, 1083
BcoDI GTCTC 3 cut(s) 557, 614, 1235
BfaI CTAG 7 cut(s) 149, 801, 834, 987, 1223, 1284, 1464
BfmI CTRYAG 2 cut(s) 433, 698
BfoI RGCGCY 1 cut(s) 677
BfuAI ACCTGC 1 cut(s) 426
BglI GCCNNNNNGGC 1 cut(s) 538
BisI GCNGC 5 cut(s) 33, 42, 45, 433, 936
BlsI GCNGC 5 cut(s) 34, 43, 46, 434, 937
Bme1390I CCNGG 5 cut(s) 49, 787, 951, 1083, 1303
Bme18I GGWCC 1 cut(s) 126
BmeRI GACNNNNNGTC 1 cut(s) 132
BmgBI CACGTC 1 cut(s) 1066
BmgT120I GGNCC 1 cut(s) 126
BmiI GGNNCC 6 cut(s) 327, 675, 714, 984, 1071, 1376
BmrFI CCNGG 5 cut(s) 49, 787, 951, 1083, 1303
BmsI GCATC 2 cut(s) 382, 721
BmtI GCTAGC 1 cut(s) 152
BpiI GAAGAC 1 cut(s) 1452
BpmI CTGGAG 1 cut(s) 563
BpuEI CTTGAG 3 cut(s) 12, 1184, 1259
BpuMI CCSGG 2 cut(s) 49, 1083
BsaHI GRCGYC 1 cut(s) 674
BsaJI CCNNGG 6 cut(s) 261, 295, 338, 858, 950, 1317
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 3 cut(s) 282, 992, 1030
Bse118I RCCGGY 1 cut(s) 1127
Bse1I ACTGG 1 cut(s) 590
BseBI CCWGG 3 cut(s) 787, 951, 1303
BseDI CCNNGG 6 cut(s) 261, 295, 338, 858, 950, 1317
BseGI GGATG 4 cut(s) 594, 677, 736, 1267
BseLI CCNNNNNNNGG 3 cut(s) 282, 992, 1030
BseMII CTCAG 4 cut(s) 408, 464, 822, 1034
BseNI ACTGG 1 cut(s) 590
BseRI GAGGAG 2 cut(s) 149, 632
BseSI GKGCMC 1 cut(s) 985
BseXI GCAGC 4 cut(s) 53, 56, 419, 922
BshFI GGCC 2 cut(s) 23, 35
BshNI GGYRCC 3 cut(s) 673, 982, 1374
BsiSI CCGG 4 cut(s) 20, 49, 1082, 1128
BslFI GGGAC 5 cut(s) 483, 502, 679, 1098, 1407
BslI CCNNNNNNNGG 3 cut(s) 282, 992, 1030
BsmAI GTCTC 3 cut(s) 557, 614, 1235
BsmBI CGTCTC 1 cut(s) 614
BsmFI GGGAC 5 cut(s) 483, 502, 679, 1098, 1407
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 2 cut(s) 23, 35
Bsp1286I GDGCHC 1 cut(s) 985
Bsp143I GATC 7 cut(s) 60, 65, 94, 325, 630, 694, 1351
BspACI CCGC 4 cut(s) 32, 39, 426, 1539
BspANI GGCC 2 cut(s) 23, 35
BspCNI CTCAG 4 cut(s) 409, 465, 821, 1035
BspHI TCATGA 1 cut(s) 819
BspLI GGNNCC 6 cut(s) 327, 675, 714, 984, 1071, 1376
BspMAI CTGCAG 2 cut(s) 437, 702
BspMI ACCTGC 1 cut(s) 426
BspOI GCTAGC 1 cut(s) 152
BspPI GGATC 6 cut(s) 55, 320, 333, 638, 702, 1359
BspQI GCTCTTC 1 cut(s) 1497
BspT107I GGYRCC 3 cut(s) 673, 982, 1374
BsrFI RCCGGY 1 cut(s) 1127
BsrI ACTGG 1 cut(s) 590
BssAI RCCGGY 1 cut(s) 1127
BssECI CCNNGG 6 cut(s) 261, 295, 338, 858, 950, 1317
BssMI GATC 7 cut(s) 60, 65, 94, 325, 630, 694, 1351
BssNI GRCGYC 1 cut(s) 674
BssT1I CCWWGG 4 cut(s) 261, 338, 858, 1317
Bst2UI CCWGG 3 cut(s) 787, 951, 1303
Bst4CI ACNGT 5 cut(s) 134, 197, 368, 670, 1370
Bst6I CTCTTC 3 cut(s) 225, 343, 1497
BstACI GRCGYC 1 cut(s) 674
BstC8I GCNNGC 1 cut(s) 150
BstDEI CTNAG 6 cut(s) 344, 417, 473, 808, 1043, 1355
BstDSI CCRYGG 1 cut(s) 295
BstF5I GGATG 4 cut(s) 594, 677, 736, 1267
BstH2I RGCGCY 1 cut(s) 677
BstHHI GCGC 1 cut(s) 676
BstKTI GATC 7 cut(s) 63, 68, 97, 328, 633, 697, 1354
BstMAI GTCTC 3 cut(s) 557, 614, 1235
BstMBI GATC 7 cut(s) 60, 65, 94, 325, 630, 694, 1351
BstMWI GCNNNNNNNGC 6 cut(s) 29, 38, 41, 432, 538, 932
BstNI CCWGG 3 cut(s) 787, 951, 1303
BstNSI RCATGY 4 cut(s) 532, 1157, 1335, 1342
BstSCI CCNGG 5 cut(s) 47, 785, 949, 1081, 1301
BstSFI CTRYAG 2 cut(s) 433, 698
BstSLI GKGCMC 1 cut(s) 985
BstV1I GCAGC 4 cut(s) 53, 56, 419, 922
BstV2I GAAGAC 1 cut(s) 1452
BstX2I RGATCY 3 cut(s) 325, 694, 1351
BstXI CCANNNNNNTGG 2 cut(s) 684, 1371
BstYI RGATCY 3 cut(s) 325, 694, 1351
BsuRI GGCC 2 cut(s) 23, 35
BtgI CCRYGG 1 cut(s) 295
BtrI CACGTC 1 cut(s) 1066
BtsCI GGATG 4 cut(s) 594, 677, 736, 1267
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 3 cut(s) 609, 1040, 1366
BveI ACCTGC 1 cut(s) 426
Cac8I GCNNGC 1 cut(s) 150
CciI TCATGA 1 cut(s) 819
CfoI GCGC 1 cut(s) 676
Cfr10I RCCGGY 1 cut(s) 1127
Cfr13I GGNCC 1 cut(s) 126
CsiI ACCWGGT 1 cut(s) 785
Csp6I GTAC 6 cut(s) 364, 439, 790, 1375, 1520, 1536
CviQI GTAC 6 cut(s) 364, 439, 790, 1375, 1520, 1536
DdeI CTNAG 6 cut(s) 344, 417, 473, 808, 1043, 1355
DinI GGCGCC 1 cut(s) 675
DpnI GATC 7 cut(s) 62, 67, 96, 327, 632, 696, 1353
DpnII GATC 7 cut(s) 60, 65, 94, 325, 630, 694, 1351
DraIII CACNNNGTG 1 cut(s) 1258
DriI GACNNNNNGTC 1 cut(s) 132
EaeI YGGCCR 1 cut(s) 21
Eam1104I CTCTTC 3 cut(s) 225, 343, 1497
Eam1105I GACNNNNNGTC 1 cut(s) 132
EarI CTCTTC 3 cut(s) 225, 343, 1497
Eco130I CCWWGG 4 cut(s) 261, 338, 858, 1317
Eco47I GGWCC 1 cut(s) 126
Eco57I CTGAAG 2 cut(s) 249, 1464
EcoRII CCWGG 3 cut(s) 785, 949, 1301
EcoT14I CCWWGG 4 cut(s) 261, 338, 858, 1317
EgeI GGCGCC 1 cut(s) 675
EheI GGCGCC 1 cut(s) 675
ErhI CCWWGG 4 cut(s) 261, 338, 858, 1317
Esp3I CGTCTC 1 cut(s) 614
FalI AAGNNNNNCTT 4 cut(s) 75, 107, 861, 893
FaqI GGGAC 5 cut(s) 483, 502, 679, 1098, 1407
FauI CCCGC 1 cut(s) 419
FauNDI CATATG 1 cut(s) 388
FbaI TGATCA 1 cut(s) 94
Fnu4HI GCNGC 5 cut(s) 33, 42, 45, 433, 936
FokI GGATG 4 cut(s) 601, 664, 743, 1274
Fsp4HI GCNGC 5 cut(s) 33, 42, 45, 433, 936
FspBI CTAG 7 cut(s) 149, 801, 834, 987, 1223, 1284, 1464
GlaI GCGC 1 cut(s) 675
GluI GCNGC 5 cut(s) 33, 42, 45, 433, 936
GsuI CTGGAG 1 cut(s) 563
HaeII RGCGCY 1 cut(s) 677
HaeIII GGCC 2 cut(s) 23, 35
HapII CCGG 4 cut(s) 20, 49, 1082, 1128
HhaI GCGC 1 cut(s) 676
Hin1I GRCGYC 1 cut(s) 674
Hin6I GCGC 1 cut(s) 674
HinP1I GCGC 1 cut(s) 674
HinfI GANTC 5 cut(s) 143, 407, 578, 874, 1359
HpaII CCGG 4 cut(s) 20, 49, 1082, 1128
HphI GGTGA 6 cut(s) 92, 380, 875, 1244, 1333, 1449
Hpy166II GTNNAC 2 cut(s) 225, 1324
Hpy188I TCNGA 9 cut(s) 65, 277, 418, 635, 682, 811, 1010, 1074, 1513
Hpy188III TCNNGA 4 cut(s) 92, 820, 1036, 1201
Hpy8I GTNNAC 2 cut(s) 225, 1324
Hpy99I CGWCG 1 cut(s) 1192
HpyAV CCTTC 5 cut(s) 621, 630, 817, 1094, 1464
HpyCH4III ACNGT 5 cut(s) 134, 197, 368, 670, 1370
HpyCH4IV ACGT 1 cut(s) 1065
HpyCH4V TGCA 7 cut(s) 377, 435, 491, 602, 700, 938, 1108
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 38, 41, 432, 538, 932
HpyF3I CTNAG 6 cut(s) 344, 417, 473, 808, 1043, 1355
HpySE526I ACGT 1 cut(s) 1065
Hsp92I GRCGYC 1 cut(s) 674
HspAI GCGC 1 cut(s) 674
KasI GGCGCC 1 cut(s) 673
KpnI GGTACC 1 cut(s) 1378
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 7 cut(s) 60, 65, 94, 325, 630, 694, 1351
LguI GCTCTTC 1 cut(s) 1497
LmnI GCTCC 3 cut(s) 429, 570, 1160
Lsp1109I GCAGC 4 cut(s) 53, 56, 419, 922
LweI GCATC 2 cut(s) 382, 721
MabI ACCWGGT 1 cut(s) 785
MaeI CTAG 7 cut(s) 149, 801, 834, 987, 1223, 1284, 1464
MaeII ACGT 1 cut(s) 1065
MaeIII GTNAC 3 cut(s) 549, 829, 1334
MalI GATC 7 cut(s) 62, 67, 96, 327, 632, 696, 1353
MboI GATC 7 cut(s) 60, 65, 94, 325, 630, 694, 1351
MboII GAAGA 9 cut(s) 158, 242, 360, 878, 1130, 1241, 1457, 1484, 1487
MfeI CAATTG 2 cut(s) 211, 1379
MflI RGATCY 3 cut(s) 325, 694, 1351
MhlI GDGCHC 1 cut(s) 985
MluCI AATT 9 cut(s) 211, 371, 535, 882, 945, 1122, 1216, 1379, 1556
Mly113I GGCGCC 1 cut(s) 674
MlyI GAGTC 2 cut(s) 401, 883
MmeI TCCRAC 2 cut(s) 690, 1138
MroXI GAANNNNTTC 2 cut(s) 87, 1449
MseI TTAA 1 cut(s) 1425
MslI CAYNNNNRTG 3 cut(s) 682, 1138, 1343
MspA1I CMGCKG 1 cut(s) 140
MspI CCGG 4 cut(s) 20, 49, 1082, 1128
MspR9I CCNGG 5 cut(s) 49, 787, 951, 1083, 1303
MunI CAATTG 2 cut(s) 211, 1379
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 3 cut(s) 787, 951, 1303
MwoI GCNNNNNNNGC 6 cut(s) 29, 38, 41, 432, 538, 932
NarI GGCGCC 1 cut(s) 674
NciI CCSGG 2 cut(s) 49, 1083
NdeI CATATG 1 cut(s) 388
NdeII GATC 7 cut(s) 60, 65, 94, 325, 630, 694, 1351
NheI GCTAGC 1 cut(s) 148
NlaIV GGNNCC 6 cut(s) 327, 675, 714, 984, 1071, 1376
NmuCI GTSAC 3 cut(s) 549, 829, 1334
NspI RCATGY 4 cut(s) 532, 1157, 1335, 1342
PagI TCATGA 1 cut(s) 819
PciI ACATGT 2 cut(s) 1153, 1338
PciSI GCTCTTC 1 cut(s) 1497
PctI GAATGC 1 cut(s) 238
PdmI GAANNNNTTC 2 cut(s) 87, 1449
PfeI GAWTC 3 cut(s) 143, 578, 1359
PkrI GCNGC 5 cut(s) 34, 43, 46, 434, 937
PleI GAGTC 2 cut(s) 401, 882
PluTI GGCGCC 1 cut(s) 677
PpsI GAGTC 2 cut(s) 401, 882
PscI ACATGT 2 cut(s) 1153, 1338
PsiI TTATAA 1 cut(s) 852
Psp6I CCWGG 3 cut(s) 785, 949, 1301
PspGI CCWGG 3 cut(s) 785, 949, 1301
PspN4I GGNNCC 6 cut(s) 327, 675, 714, 984, 1071, 1376
PspPI GGNCC 1 cut(s) 126
PstI CTGCAG 2 cut(s) 437, 702
PsuI RGATCY 3 cut(s) 325, 694, 1351
PvuII CAGCTG 1 cut(s) 140
RsaI GTAC 6 cut(s) 365, 440, 791, 1376, 1521, 1537
RsaNI GTAC 6 cut(s) 364, 439, 790, 1375, 1520, 1536
RseI CAYNNNNRTG 3 cut(s) 682, 1138, 1343
SapI GCTCTTC 1 cut(s) 1497
SaqAI TTAA 1 cut(s) 1425
SatI GCNGC 5 cut(s) 33, 42, 45, 433, 936
Sau3AI GATC 7 cut(s) 60, 65, 94, 325, 630, 694, 1351
Sau96I GGNCC 1 cut(s) 126
SchI GAGTC 2 cut(s) 401, 883
ScrFI CCNGG 5 cut(s) 49, 787, 951, 1083, 1303
SduI GDGCHC 1 cut(s) 985
SexAI ACCWGGT 1 cut(s) 785
SfaNI GCATC 2 cut(s) 382, 721
SfcI CTRYAG 2 cut(s) 433, 698
SfoI GGCGCC 1 cut(s) 675
SinI GGWCC 1 cut(s) 126
SmiMI CAYNNNNRTG 3 cut(s) 682, 1138, 1343
SmlI CTYRAG 3 cut(s) 27, 1199, 1238
SmoI CTYRAG 3 cut(s) 27, 1199, 1238
Sse9I AATT 9 cut(s) 211, 371, 535, 882, 945, 1122, 1216, 1379, 1556
SsiI CCGC 4 cut(s) 32, 39, 426, 1539
SspDI GGCGCC 1 cut(s) 673
SspMI CTAG 7 cut(s) 149, 801, 834, 987, 1223, 1284, 1464
StyD4I CCNGG 5 cut(s) 47, 785, 949, 1081, 1301
StyI CCWWGG 4 cut(s) 261, 338, 858, 1317
TaaI ACNGT 5 cut(s) 134, 197, 368, 670, 1370
TaiI ACGT 1 cut(s) 1068
TaqI TCGA 1 cut(s) 399
TasI AATT 9 cut(s) 211, 371, 535, 882, 945, 1122, 1216, 1379, 1556
TatI WGTACW 1 cut(s) 1519
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 3 cut(s) 143, 578, 1359
Tru1I TTAA 1 cut(s) 1425
Tru9I TTAA 1 cut(s) 1425
TscAI CASTG 3 cut(s) 609, 1047, 1373
TseFI GTSAC 3 cut(s) 549, 829, 1334
TseI GCWGC 4 cut(s) 41, 44, 432, 935
Tsp45I GTSAC 3 cut(s) 549, 829, 1334
TspDTI ATGAA 5 cut(s) 836, 963, 1113, 1220, 1562
TspRI CASTG 3 cut(s) 609, 1047, 1373
VpaK11BI GGWCC 1 cut(s) 126
XapI RAATTY 2 cut(s) 371, 1556
XceI RCATGY 4 cut(s) 532, 1157, 1335, 1342
XcmI CCANNNNNNNNNTGG 1 cut(s) 336
XmnI GAANNNNTTC 2 cut(s) 87, 1449
XspI CTAG 7 cut(s) 149, 801, 834, 987, 1223, 1284, 1464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.