Rmu_sc0005112.1_g000002

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005112.1
OG0016345
Physical Location & Seq
Reverse (-)
1813 .. 11080
9268 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 978 bp
atgttgtttgagaaatattcattcgagagtaaagtgtggcatacaagcgaacccaaccccaggggaggcgaggacttggttctgatcgaaataaaggtctacactaagatcggagccgccttgaggattggttctccaacgaagtatggcgaaccataccacagtgaaggtagagaactcatcatttctaatctctctcgtagcgagctattatccaataagagtgagtatacatggagaatggtcgagttcttttcaggcacatttaaggaggagccactccccgatgatgtgcgacgatcttgtattgaagaagtattccgggtggtcgaaaaagatctagtggtggcctcatcattttctgttattcatgtcgacatattggaagtggttgaccacgtgcttgagtctgctgccgttgcctttctcgacgatgcgatatcatcaatcgagggtttggagaaagtgagagttgatagctctttcctggggttagaagactcttgtgctatttgtatggaggacctaattgacagagacggcaagaaacaatttaaacctaagaggaagagcaagaggctgcttggatttcaaagcctcagcaccaccattgctaaaaggccaagaaaagagccgccccgtttgaggatactaaatagccgggcaattgaggcggcagagaaggaaaggccagttccagacattaggagtggagacgttgtgcaaattaaactggaggttcctgaaaacaagcgtaggttgtctatctataaaggtatcgttatttcaaagcaaaatgctggcattcatacaacatttcgcattcggaggattattgctggcataggggttgagattgttttccctctatactcaccgaacattaaggagttgaaggtacttagtcataggaaggtcagaagggcaaggctgtactatttgagagacaaacttcctaggttctcgactttcaagtga

Protein Analysis

325

Amino Acids

37.25

Weight (kDa)

9.44

Isoelectric Point (pI)

56.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L19 PF01245 222 - 319 1e-30 Ribosomal protein L19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 99, 230, 375
AciI CCGC 3 cut(s) 117, 635, 674
AcvI CACGTG 1 cut(s) 400
AfaI GTAC 2 cut(s) 900, 935
AfiI CCNNNNNNNGG 4 cut(s) 60, 65, 123, 645
AgsI TTSAA 5 cut(s) 311, 593, 789, 895, 973
AjnI CCWGG 2 cut(s) 59, 486
AluBI AGCT 2 cut(s) 208, 480
AluI AGCT 2 cut(s) 208, 480
Alw26I GTCTC 3 cut(s) 531, 708, 939
AoxI GGCC 3 cut(s) 348, 620, 689
ApeKI GCWGC 2 cut(s) 413, 580
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
AspA2I CCTAGG 1 cut(s) 956
AspS9I GGNCC 1 cut(s) 523
AsuC2I CCSGG 2 cut(s) 323, 662
AsuHPI GGTGA 1 cut(s) 867
AvaII GGWCC 1 cut(s) 523
AvrII CCTAGG 1 cut(s) 956
BbrPI CACGTG 1 cut(s) 400
BbsI GAAGAC 1 cut(s) 504
BbvCI CCTCAGC 1 cut(s) 599
BbvI GCAGC 2 cut(s) 400, 567
BceAI ACGGC 2 cut(s) 401, 556
BciT130I CCWGG 2 cut(s) 61, 488
BciVI GTATCC 1 cut(s) 642
BcnI CCSGG 2 cut(s) 323, 662
BcoDI GTCTC 3 cut(s) 531, 708, 939
BfaI CTAG 2 cut(s) 341, 957
BfuI GTATCC 1 cut(s) 642
BglII AGATCT 1 cut(s) 337
BisI GCNGC 5 cut(s) 117, 414, 581, 635, 675
BlnI CCTAGG 1 cut(s) 956
BlsI GCNGC 5 cut(s) 118, 415, 582, 636, 676
Bme1390I CCNGG 4 cut(s) 61, 323, 488, 662
Bme18I GGWCC 1 cut(s) 523
BmgT120I GGNCC 1 cut(s) 523
BmiI GGNNCC 3 cut(s) 115, 276, 741
BmrFI CCNGG 4 cut(s) 61, 323, 488, 662
BmsI GCATC 1 cut(s) 424
BpiI GAAGAC 1 cut(s) 504
BpmI CTGGAG 1 cut(s) 755
Bpu10I CCTNAGC 1 cut(s) 599
BpuEI CTTGAG 2 cut(s) 142, 425
BpuMI CCSGG 2 cut(s) 323, 662
BsaAI YACGTR 1 cut(s) 400
BsaJI CCNNGG 4 cut(s) 59, 60, 487, 956
BsaXI ACNNNNNCTCC 2 cut(s) 705, 735
Bsc4I CCNNNNNNNGG 4 cut(s) 60, 65, 123, 645
Bse1I ACTGG 2 cut(s) 692, 738
Bse3DI GCAATG 1 cut(s) 609
BseBI CCWGG 2 cut(s) 61, 488
BseDI CCNNGG 4 cut(s) 59, 60, 487, 956
BseLI CCNNNNNNNGG 4 cut(s) 60, 65, 123, 645
BseMI GCAATG 1 cut(s) 609
BseMII CTCAG 1 cut(s) 613
BseNI ACTGG 2 cut(s) 692, 738
BseRI GAGGAG 1 cut(s) 287
BseXI GCAGC 2 cut(s) 400, 567
BshFI GGCC 3 cut(s) 350, 622, 691
BsiSI CCGG 2 cut(s) 322, 661
BslI CCNNNNNNNGG 4 cut(s) 60, 65, 123, 645
BsmAI GTCTC 3 cut(s) 531, 708, 939
BsmBI CGTCTC 2 cut(s) 531, 708
BsmI GAATGC 2 cut(s) 804, 822
BsnI GGCC 3 cut(s) 350, 622, 691
Bsp143I GATC 4 cut(s) 84, 108, 299, 337
BspACI CCGC 3 cut(s) 117, 635, 674
BspANI GGCC 3 cut(s) 350, 622, 691
BspCNI CTCAG 1 cut(s) 612
BspLI GGNNCC 3 cut(s) 115, 276, 741
BspQI GCTCTTC 1 cut(s) 563
BsrDI GCAATG 1 cut(s) 609
BsrI ACTGG 2 cut(s) 692, 738
BssECI CCNNGG 4 cut(s) 59, 60, 487, 956
BssMI GATC 4 cut(s) 84, 108, 299, 337
BssNAI GTATAC 1 cut(s) 231
BssT1I CCWWGG 1 cut(s) 956
Bst1107I GTATAC 1 cut(s) 231
Bst2UI CCWGG 2 cut(s) 61, 488
Bst4CI ACNGT 1 cut(s) 164
Bst6I CTCTTC 1 cut(s) 563
BstBAI YACGTR 1 cut(s) 400
BstC8I GCNNGC 3 cut(s) 206, 802, 841
BstDEI CTNAG 4 cut(s) 105, 561, 599, 902
BstKTI GATC 4 cut(s) 87, 111, 302, 340
BstMAI GTCTC 3 cut(s) 531, 708, 939
BstMBI GATC 4 cut(s) 84, 108, 299, 337
BstMWI GCNNNNNNNGC 2 cut(s) 419, 671
BstNI CCWGG 2 cut(s) 61, 488
BstSCI CCNGG 4 cut(s) 59, 321, 486, 660
BstV1I GCAGC 2 cut(s) 400, 567
BstV2I GAAGAC 1 cut(s) 504
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BstZ17I GTATAC 1 cut(s) 231
BsuI GTATCC 1 cut(s) 642
BsuRI GGCC 3 cut(s) 350, 622, 691
BtsIMutI CAGTG 1 cut(s) 169
Cac8I GCNNGC 3 cut(s) 206, 802, 841
Cfr13I GGNCC 1 cut(s) 523
Csp6I GTAC 2 cut(s) 899, 934
CviAII CATG 2 cut(s) 234, 371
CviQI GTAC 2 cut(s) 899, 934
DdeI CTNAG 4 cut(s) 105, 561, 599, 902
DpnI GATC 4 cut(s) 86, 110, 301, 339
DpnII GATC 4 cut(s) 84, 108, 299, 337
DraI TTTAAA 1 cut(s) 556
Eam1104I CTCTTC 1 cut(s) 563
EarI CTCTTC 1 cut(s) 563
Eco130I CCWWGG 1 cut(s) 956
Eco32I GATATC 1 cut(s) 441
Eco47I GGWCC 1 cut(s) 523
Eco72I CACGTG 1 cut(s) 400
EcoO109I RGGNCCY 1 cut(s) 523
EcoRII CCWGG 2 cut(s) 59, 486
EcoRV GATATC 1 cut(s) 441
EcoT14I CCWWGG 1 cut(s) 956
ErhI CCWWGG 1 cut(s) 956
Esp3I CGTCTC 2 cut(s) 531, 708
FaeI CATG 2 cut(s) 237, 374
FatI CATG 2 cut(s) 233, 370
FblI GTMKAC 3 cut(s) 99, 230, 375
Fnu4HI GCNGC 5 cut(s) 117, 414, 581, 635, 675
Fsp4HI GCNGC 5 cut(s) 117, 414, 581, 635, 675
FspBI CTAG 2 cut(s) 341, 957
GluI GCNGC 5 cut(s) 117, 414, 581, 635, 675
GsuI CTGGAG 1 cut(s) 755
HaeIII GGCC 3 cut(s) 350, 622, 691
HapII CCGG 2 cut(s) 322, 661
Hin1II CATG 2 cut(s) 237, 374
HincII GTYRAC 2 cut(s) 376, 394
HindII GTYRAC 2 cut(s) 376, 394
HinfI GANTC 2 cut(s) 407, 500
HpaII CCGG 2 cut(s) 322, 661
HphI GGTGA 1 cut(s) 867
Hpy166II GTNNAC 4 cut(s) 100, 231, 376, 394
Hpy188I TCNGA 4 cut(s) 84, 113, 828, 920
Hpy188III TCNNGA 5 cut(s) 25, 428, 698, 743, 964
Hpy8I GTNNAC 4 cut(s) 100, 231, 376, 394
Hpy99I CGWCG 2 cut(s) 300, 434
HpyAV CCTTC 5 cut(s) 161, 676, 889, 907, 915
HpyCH4III ACNGT 1 cut(s) 164
HpyCH4IV ACGT 2 cut(s) 399, 717
HpyCH4V TGCA 1 cut(s) 724
HpyF10VI GCNNNNNNNGC 2 cut(s) 419, 671
HpyF3I CTNAG 4 cut(s) 105, 561, 599, 902
HpySE526I ACGT 2 cut(s) 399, 717
Hsp92II CATG 2 cut(s) 237, 374
Kzo9I GATC 4 cut(s) 84, 108, 299, 337
LguI GCTCTTC 1 cut(s) 563
LmnI GCTCC 2 cut(s) 113, 274
Lsp1109I GCAGC 2 cut(s) 400, 567
LweI GCATC 1 cut(s) 424
MaeI CTAG 2 cut(s) 341, 957
MaeII ACGT 2 cut(s) 399, 717
MalI GATC 4 cut(s) 86, 110, 301, 339
MboI GATC 4 cut(s) 84, 108, 299, 337
MboII GAAGA 3 cut(s) 323, 509, 580
MfeI CAATTG 1 cut(s) 666
MflI RGATCY 1 cut(s) 337
MluCI AATT 4 cut(s) 528, 551, 666, 726
MlyI GAGTC 2 cut(s) 416, 494
MmeI TCCRAC 1 cut(s) 161
MseI TTAA 4 cut(s) 267, 555, 729, 885
MspI CCGG 2 cut(s) 322, 661
MspR9I CCNGG 4 cut(s) 61, 323, 488, 662
MunI CAATTG 1 cut(s) 666
Mva1269I GAATGC 2 cut(s) 804, 822
MvaI CCWGG 2 cut(s) 61, 488
MwoI GCNNNNNNNGC 2 cut(s) 419, 671
NciI CCSGG 2 cut(s) 323, 662
NdeII GATC 4 cut(s) 84, 108, 299, 337
NlaIII CATG 2 cut(s) 237, 374
NlaIV GGNNCC 3 cut(s) 115, 276, 741
PasI CCCWGGG 1 cut(s) 60
PciSI GCTCTTC 1 cut(s) 563
PctI GAATGC 2 cut(s) 804, 822
PkrI GCNGC 5 cut(s) 118, 415, 582, 636, 676
PleI GAGTC 2 cut(s) 415, 494
PmaCI CACGTG 1 cut(s) 400
PmlI CACGTG 1 cut(s) 400
PpsI GAGTC 2 cut(s) 415, 494
Ppu21I YACGTR 1 cut(s) 400
PpuMI RGGWCCY 1 cut(s) 523
Psp5II RGGWCCY 1 cut(s) 523
Psp6I CCWGG 2 cut(s) 59, 486
PspCI CACGTG 1 cut(s) 400
PspGI CCWGG 2 cut(s) 59, 486
PspN4I GGNNCC 3 cut(s) 115, 276, 741
PspPI GGNCC 1 cut(s) 523
PspPPI RGGWCCY 1 cut(s) 523
PsuI RGATCY 1 cut(s) 337
RsaI GTAC 2 cut(s) 900, 935
RsaNI GTAC 2 cut(s) 899, 934
SalI GTCGAC 1 cut(s) 374
SapI GCTCTTC 1 cut(s) 563
SaqAI TTAA 4 cut(s) 267, 555, 729, 885
SatI GCNGC 5 cut(s) 117, 414, 581, 635, 675
Sau3AI GATC 4 cut(s) 84, 108, 299, 337
Sau96I GGNCC 1 cut(s) 523
SchI GAGTC 2 cut(s) 416, 494
ScrFI CCNGG 4 cut(s) 61, 323, 488, 662
SfaNI GCATC 1 cut(s) 424
SinI GGWCC 1 cut(s) 523
SmlI CTYRAG 2 cut(s) 121, 404
SmoI CTYRAG 2 cut(s) 121, 404
Sse9I AATT 4 cut(s) 528, 551, 666, 726
SsiI CCGC 3 cut(s) 117, 635, 674
SspI AATATT 1 cut(s) 17
SspMI CTAG 2 cut(s) 341, 957
StyD4I CCNGG 4 cut(s) 59, 321, 486, 660
StyI CCWWGG 1 cut(s) 956
TaaI ACNGT 1 cut(s) 164
TaiI ACGT 2 cut(s) 402, 720
TaqI TCGA 8 cut(s) 24, 87, 246, 330, 375, 429, 450, 965
TasI AATT 4 cut(s) 528, 551, 666, 726
TatI WGTACW 1 cut(s) 933
TauI GCSGC 3 cut(s) 119, 637, 677
Tru1I TTAA 4 cut(s) 267, 555, 729, 885
Tru9I TTAA 4 cut(s) 267, 555, 729, 885
TscAI CASTG 1 cut(s) 169
TseI GCWGC 2 cut(s) 413, 580
TspDTI ATGAA 3 cut(s) 9, 359, 797
TspRI CASTG 1 cut(s) 169
VpaK11BI GGWCC 1 cut(s) 523
XmaJI CCTAGG 1 cut(s) 956
XmiI GTMKAC 3 cut(s) 99, 230, 375
XspI CTAG 2 cut(s) 341, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.