Rmu_sc0005297.1_g000028

Protein BREAST CANCER SUSCEPTIBILITY 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005297.1
OG0007909
Physical Location & Seq
Reverse (-)
127334 .. 133833
6500 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2388 bp
atgattacattaatctggttaagcaccacctctccggcgggaaattctctcaaaacaaaagaggaaaagggcgccactcccactcaaatattccagcaccccgctcttttcccactcgggcaaaaacacctccgccctccacgtgtccctcctcacctccaaaccaatagaaatccgccgcctcataaatactccacactcttccacaccaccccttcagtcacttctctctctctctctgcacggttcccaatggcccagtccgccaagcagtcttcagattccggaaactgccgcgacggcgtcgtttcgccgatgaacaagaaccccctcacgtttcatctccagaagctcggcctcgagctcacttgccctctctgcctagggttttttaatcggccgacattgcttccctgcaatcacatcttctgcaacaactgtgtgcccaatcggaccaattttggagcagagtgtcctctgtgtaatatccaagttcctggtcaagatctgaggcatgcgcctttcatggaaagcatagtagcaatctacaaaagcatgaaggccacttactgtaccaactcctcccagcctatatcctcagatgttagaacagcttcgggtcaaaacccggtttcacctgatgtcagttttgctgggaaatcgactaaagaaccgtttgacaattgccaagagggtaaatcagaaattgctcagaccattgtttcatcaaggggtaataagcgagtatgggttccatgtagtcagaaacattctgttacagatggaattggcaagcatggcaaagtagataactgtagcatagcaatagatggtaacagcgaagaattcgagttgggtagaggacgtaaatttaatggtggattaaatcccaattcaccattaccaagttctcaaataagatctgtgggactggatgagtgtatgacttcagaaagaggcatgaatccagtagtgcaggcgttgctggatagtcctccttcatttggtgatgccaaatgttcagataatgataatagctttgaccagtgctctgagcaaatttcagacagtttgatcagggggtcaatgaggaaaattgatgatagaacagggcagatgaggcctgatagttctgcatctgatactgatggccatttgagggaactcaagagacagaagaaactggactatgggctagtctcagggactaattctgagctggaacttaaattcagaacaccttctaatgagtctttcacaaataacaccatttgtgcattttgccagtcttacacaatctcagaggtcactgggccaatgttgcactattccaagggaaaatttgtggagggagatgaggcagctgtttcagatgtcatacatgttcacaaaatatgcattgattgggcaccacaagtgttctttaaagatgaaaccattaaaaatttgaaggcggaagtgacaaggggtgcaaaattaaaatgcaccaaatgtgggataaagggagcagccttgggatgctttgtgaaatcctgccgaaaaagctatcatgttacctgtgcaattgaaatttctaagtgccgttgggattatgaaagctttcttctgctgtgtccagcacattgttcagttaaatttcccaatgagaagttgaatcgtaacaagcacaaaagttgtcagctctccgacagtagagtcgcttcaaatggagtgaacaactgggttttatgcccttctggtttgtcttctgaagagaagatttttcttttcaaatttgctgagatgaatggtgcaactttgtccaagagctggacacctgatgttacacatgtgattgctgccctggatggggatggtgcatatgtccggacgtttaaaatttgtatggccattttggcaggaaaatggatagtgacaattgactgggtaaaagcatgtatggaaactaagcatcatgtggacgaagaaccttatgaagttagtcttgataactatggttgtcgcagtggtgccaaagctggcaggcttagggcattaagcaatgatccgaagctctttaatggtttgaatttctattttgctggtgattttatactggaacggaaggaagactacaaagaattggtcatagctgcaggaggtactattttcaacagtaaggaagatgtgcttgaagcaagttgtcatgaaaagggaactccgaggacactagttgtgtacaaccttgaccccccacctgggtgcaagttgggagaagaagtttcaatactttggcaaaggtctaacgaggcacaagatatagctgcgaaaattggagctgaatacgtctttcacacatggttgttggagtcaattgcgaggtatgagttgcagccaattgcatgttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000152 GO:0000724 GO:0000725 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009314 GO:0009628 GO:0009653 GO:0009888 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009934 GO:0009987 GO:0010015 GO:0010073 GO:0010074 GO:0010078 GO:0010212 GO:0010332 GO:0010468 GO:0010492 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010565 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010638 GO:0010639 GO:0016020 GO:0016043 GO:0016567 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0018022 GO:0018023 GO:0018193 GO:0018205 GO:0019216 GO:0019217 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019827 GO:0022603 GO:0022607 GO:0022622 GO:0031056 GO:0031057 GO:0031058 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031401 GO:0031436 GO:0032259 GO:0032268 GO:0032269 GO:0032270 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033043 GO:0033044 GO:0033554 GO:0034622 GO:0034641 GO:0034968 GO:0035065 GO:0035066 GO:0035067 GO:0036211 GO:0042127 GO:0042304 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045717 GO:0045787 GO:0045833 GO:0045892 GO:0045893 GO:0045922 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046890 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051055 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051568 GO:0051716 GO:0051726 GO:0060255 GO:0062012 GO:0062014 GO:0065003 GO:0065004 GO:0065007 GO:0070531 GO:0070647 GO:0071156 GO:0071158 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071704 GO:0071824 GO:0071840 GO:0071944 GO:0080090 GO:0080182 GO:0090068 GO:0090304 GO:0097159 GO:0098727 GO:0099402 GO:0104004 GO:0140096 GO:1901360 GO:1901363 GO:1901564 GO:1901983 GO:1901984 GO:1901985 GO:1902275 GO:1902494 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:1905269 GO:1905392 GO:1990234 GO:2000112 GO:2000113 GO:2000756 GO:2000757 GO:2000758 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03440 ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map03440 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169
Pfam Domains
Protein Families
BRCT BRCT_2 zf-C3HC4 zf-C3HC4_2 zf-C3HC4_3 zf-HC5HC2H zf-RING_UBOX

Protein Analysis

795

Amino Acids

87.74

Weight (kDa)

8.15

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4 PF00097 124 - 161 2.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 124 - 161 3.4e-06 Zinc finger, C3HC4 type (RING finger)
zf-HC5HC2H_2 PF13832 456 - 523 2.5e-07 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 463 - 541 3.3e-09 PHD-like zinc-binding domain
BRCT PF00533 577 - 647 2e-09 BRCA1 C Terminus (BRCT) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1694
AccB1I GGYRCC 3 cut(s) 71, 1408, 2009
AccB7I CCANNNNNTGG 1 cut(s) 1809
AccBSI CCGCTC 1 cut(s) 104
AccII CGCG 1 cut(s) 297
AccIII TCCGGA 2 cut(s) 284, 1866
AciI CCGC 8 cut(s) 38, 102, 133, 176, 179, 264, 295, 1454
AclWI GGATC 1 cut(s) 2039
AcoI YGGCCR 3 cut(s) 398, 1150, 1887
AcuI CTGAAG 4 cut(s) 201, 261, 933, 1770
AcvI CACGTG 1 cut(s) 143
AcyI GRCGYC 2 cut(s) 72, 303
AfaI GTAC 3 cut(s) 574, 2143, 2219
AfiI CCNNNNNNNGG 8 cut(s) 1004, 1159, 1494, 1809, 1848, 1849, 2237, 2238
AflIII ACRYGT 3 cut(s) 142, 1381, 1828
AgsI TTSAA 9 cut(s) 1450, 1568, 1654, 1704, 1771, 2068, 2152, 2174, 2265
AhlI ACTAGT 1 cut(s) 2209
AjnI CCWGG 3 cut(s) 496, 1842, 2236
AleI CACNNNNGTG 1 cut(s) 2239
Alw21I GWGCWC 2 cut(s) 366, 1052
Alw26I GTCTC 2 cut(s) 1165, 1204
AlwI GGATC 1 cut(s) 2039
Ama87I CYCGRG 2 cut(s) 116, 359
Aor13HI TCCGGA 2 cut(s) 284, 1866
AoxI GGCC 8 cut(s) 255, 355, 398, 561, 1121, 1150, 1313, 1887
ApeKI GCWGC 6 cut(s) 1361, 1508, 1838, 2132, 2303, 2371
ArsI GACNNNNNNTTYG 2 cut(s) 2109, 2141
AseI ATTAAT 1 cut(s) 11
Asp700I GAANNNNTTC 3 cut(s) 613, 1240, 1599
AspA2I CCTAGG 1 cut(s) 382
AspLEI GCGC 2 cut(s) 74, 520
AspS9I GGNCC 3 cut(s) 256, 453, 1313
AsuC2I CCSGG 1 cut(s) 629
AsuHPI GGTGA 5 cut(s) 146, 627, 888, 1019, 2096
AvaI CYCGRG 2 cut(s) 116, 359
AvaII GGWCC 1 cut(s) 453
AvrII CCTAGG 1 cut(s) 382
BaeGI GKGCMC 2 cut(s) 447, 1411
BalI TGGCCA 2 cut(s) 1152, 1889
BanI GGYRCC 3 cut(s) 71, 1408, 2009
BanII GRGCYC 1 cut(s) 366
BarI GAAGNNNNNNTAC 8 cut(s) 551, 583, 1684, 1716, 2096, 2128, 2250, 2282
BbrPI CACGTG 1 cut(s) 143
BbsI GAAGAC 3 cut(s) 267, 1737, 2115
Bbv12I GWGCWC 2 cut(s) 366, 1052
BbvI GCAGC 6 cut(s) 1373, 1520, 1825, 2119, 2290, 2383
BccI CCATC 5 cut(s) 776, 824, 1142, 1841, 1847
BceAI ACGGC 2 cut(s) 316, 1566
BciT130I CCWGG 3 cut(s) 498, 1844, 2238
BclI TGATCA 1 cut(s) 1074
BcnI CCSGG 1 cut(s) 629
BcoDI GTCTC 2 cut(s) 1165, 1204
BcuI ACTAGT 1 cut(s) 2209
BfaI CTAG 3 cut(s) 383, 1196, 2210
BfmI CTRYAG 2 cut(s) 814, 2133
BfoI RGCGCY 1 cut(s) 75
BglI GCCNNNNNGGC 2 cut(s) 300, 1895
BglII AGATCT 2 cut(s) 505, 920
BisI GCNGC 8 cut(s) 179, 295, 1362, 1509, 1839, 2133, 2304, 2372
BlnI CCTAGG 1 cut(s) 382
BlsI GCNGC 8 cut(s) 180, 296, 1363, 1510, 1840, 2134, 2305, 2373
Bme1390I CCNGG 4 cut(s) 498, 629, 1844, 2238
Bme18I GGWCC 1 cut(s) 453
BmeT110I CYCGRG 2 cut(s) 116, 359
BmgT120I GGNCC 3 cut(s) 256, 453, 1313
BmiI GGNNCC 5 cut(s) 73, 248, 753, 1410, 2011
BmrFI CCNGG 4 cut(s) 498, 629, 1844, 2238
BmrI ACTGGG 4 cut(s) 253, 1320, 1729, 1933
BmsI GCATC 4 cut(s) 1000, 1145, 1508, 1960
BmuI ACTGGG 4 cut(s) 253, 1320, 1729, 1933
BpiI GAAGAC 3 cut(s) 267, 1737, 2115
BpmI CTGGAG 1 cut(s) 329
Bpu10I CCTNAGC 1 cut(s) 2027
BpuEI CTTGAG 1 cut(s) 1151
BpuMI CCSGG 1 cut(s) 629
BsaAI YACGTR 1 cut(s) 143
BsaHI GRCGYC 2 cut(s) 72, 303
BsaJI CCNNGG 6 cut(s) 382, 1332, 1512, 1842, 2201, 2237
BsaWI WCCGGW 2 cut(s) 284, 1866
BsaXI ACNNNNNCTCC 4 cut(s) 16, 46, 2244, 2274
Bsc4I CCNNNNNNNGG 8 cut(s) 1004, 1159, 1494, 1809, 1848, 1849, 2237, 2238
Bse3DI GCAATG 2 cut(s) 404, 2047
BseAI TCCGGA 2 cut(s) 284, 1866
BseBI CCWGG 3 cut(s) 498, 1844, 2238
BseDI CCNNGG 6 cut(s) 382, 1332, 1512, 1842, 2201, 2237
BseGI GGATG 4 cut(s) 940, 1523, 1852, 1858
BseLI CCNNNNNNNGG 8 cut(s) 1004, 1159, 1494, 1809, 1848, 1849, 2237, 2238
BseMI GCAATG 2 cut(s) 404, 2047
BseMII CTCAG 8 cut(s) 500, 612, 725, 1044, 1206, 1215, 1314, 1770
BseRI GAGGAG 2 cut(s) 141, 571
BseSI GKGCMC 2 cut(s) 447, 1411
BseX3I CGGCCG 1 cut(s) 398
BseXI GCAGC 6 cut(s) 1373, 1520, 1825, 2119, 2290, 2383
BseYI CCCAGC 2 cut(s) 585, 653
BsgI GTGCAG 2 cut(s) 225, 995
Bsh1236I CGCG 1 cut(s) 297
Bsh1285I CGRYCG 1 cut(s) 401
BshFI GGCC 8 cut(s) 257, 357, 400, 563, 1123, 1152, 1315, 1889
BshNI GGYRCC 3 cut(s) 71, 1408, 2009
BsiEI CGRYCG 1 cut(s) 401
BsiHKAI GWGCWC 2 cut(s) 366, 1052
BsiHKCI CYCGRG 2 cut(s) 116, 359
BsiSI CCGG 4 cut(s) 35, 285, 629, 1867
BslFI GGGAC 3 cut(s) 131, 942, 1219
BslI CCNNNNNNNGG 8 cut(s) 1004, 1159, 1494, 1809, 1848, 1849, 2237, 2238
BsmAI GTCTC 2 cut(s) 1165, 1204
BsmFI GGGAC 3 cut(s) 131, 942, 1219
BsnI GGCC 8 cut(s) 257, 357, 400, 563, 1123, 1152, 1315, 1889
BsoBI CYCGRG 2 cut(s) 116, 359
Bsp1286I GDGCHC 4 cut(s) 366, 447, 1052, 1411
Bsp13I TCCGGA 2 cut(s) 284, 1866
Bsp1407I TGTACA 1 cut(s) 2217
Bsp143I GATC 4 cut(s) 505, 920, 1074, 2044
BspACI CCGC 8 cut(s) 38, 102, 133, 176, 179, 264, 295, 1454
BspANI GGCC 8 cut(s) 257, 357, 400, 563, 1123, 1152, 1315, 1889
BspCNI CTCAG 8 cut(s) 501, 611, 724, 1045, 1207, 1214, 1313, 1771
BspEI TCCGGA 2 cut(s) 284, 1866
BspFNI CGCG 1 cut(s) 297
BspHI TCATGA 1 cut(s) 2185
BspLI GGNNCC 5 cut(s) 73, 248, 753, 1410, 2011
BspMAI CTGCAG 1 cut(s) 2137
BspPI GGATC 1 cut(s) 2039
BspT107I GGYRCC 3 cut(s) 71, 1408, 2009
BsrBI CCGCTC 1 cut(s) 104
BsrDI GCAATG 2 cut(s) 404, 2047
BsrGI TGTACA 1 cut(s) 2217
BssECI CCNNGG 6 cut(s) 382, 1332, 1512, 1842, 2201, 2237
BssMI GATC 4 cut(s) 505, 920, 1074, 2044
BssNI GRCGYC 2 cut(s) 72, 303
BssT1I CCWWGG 3 cut(s) 382, 1332, 1512
Bst2UI CCWGG 3 cut(s) 498, 1844, 2238
Bst4CI ACNGT 8 cut(s) 246, 440, 572, 675, 815, 1070, 1691, 2156
Bst6I CTCTTC 2 cut(s) 206, 1746
BstACI GRCGYC 2 cut(s) 72, 303
BstAPI GCANNNNNTGC 1 cut(s) 982
BstAUI TGTACA 1 cut(s) 2217
BstBAI YACGTR 1 cut(s) 143
BstC8I GCNNGC 5 cut(s) 516, 794, 978, 2020, 2024
BstF5I GGATG 4 cut(s) 940, 1523, 1852, 1858
BstFNI CGCG 1 cut(s) 297
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 2 cut(s) 74, 520
BstKTI GATC 4 cut(s) 508, 923, 1077, 2047
BstMAI GTCTC 2 cut(s) 1165, 1204
BstMBI GATC 4 cut(s) 505, 920, 1074, 2044
BstMCI CGRYCG 1 cut(s) 401
BstNI CCWGG 3 cut(s) 498, 1844, 2238
BstNSI RCATGY 5 cut(s) 518, 1385, 1832, 1938, 2385
BstSCI CCNGG 4 cut(s) 496, 627, 1842, 2236
BstSFI CTRYAG 2 cut(s) 814, 2133
BstSLI GKGCMC 2 cut(s) 447, 1411
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 6 cut(s) 1373, 1520, 1825, 2119, 2290, 2383
BstV2I GAAGAC 3 cut(s) 267, 1737, 2115
BstX2I RGATCY 2 cut(s) 505, 920
BstXI CCANNNNNNTGG 1 cut(s) 497
BstYI RGATCY 2 cut(s) 505, 920
BstZI CGGCCG 1 cut(s) 398
BsuRI GGCC 8 cut(s) 257, 357, 400, 563, 1123, 1152, 1315, 1889
BtsCI GGATG 4 cut(s) 940, 1523, 1852, 1858
BtsI GCAGTG 1 cut(s) 2011
BtsIMutI CAGTG 3 cut(s) 1052, 1308, 2011
Cac8I GCNNGC 5 cut(s) 516, 794, 978, 2020, 2024
CciI TCATGA 1 cut(s) 2185
CfoI GCGC 2 cut(s) 74, 520
Cfr13I GGNCC 3 cut(s) 256, 453, 1313
CseI GACGC 1 cut(s) 292
Csp6I GTAC 3 cut(s) 573, 2142, 2218
CviQI GTAC 3 cut(s) 573, 2142, 2218
DinI GGCGCC 1 cut(s) 73
DpnI GATC 4 cut(s) 507, 922, 1076, 2046
DpnII GATC 4 cut(s) 505, 920, 1074, 2044
DraI TTTAAA 2 cut(s) 1426, 1876
DrdI GACNNNNNNGTC 1 cut(s) 1694
DseDI GACNNNNNNGTC 1 cut(s) 1694
EaeI YGGCCR 3 cut(s) 398, 1150, 1887
EagI CGGCCG 1 cut(s) 398
Eam1104I CTCTTC 2 cut(s) 206, 1746
EarI CTCTTC 2 cut(s) 206, 1746
EciI GGCGGA 4 cut(s) 122, 165, 253, 1469
Ecl136II GAGCTC 1 cut(s) 364
EclXI CGGCCG 1 cut(s) 398
Eco130I CCWWGG 3 cut(s) 382, 1332, 1512
Eco147I AGGCCT 1 cut(s) 1123
Eco24I GRGCYC 1 cut(s) 366
Eco47I GGWCC 1 cut(s) 453
Eco52I CGGCCG 1 cut(s) 398
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 4 cut(s) 201, 261, 933, 1770
Eco72I CACGTG 1 cut(s) 143
Eco88I CYCGRG 2 cut(s) 116, 359
EcoICRI GAGCTC 1 cut(s) 364
EcoRI GAATTC 1 cut(s) 845
EcoRII CCWGG 3 cut(s) 496, 1842, 2236
EcoT14I CCWWGG 3 cut(s) 382, 1332, 1512
EcoT22I ATGCAT 1 cut(s) 1400
EcoT38I GRGCYC 1 cut(s) 366
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 3 cut(s) 382, 1332, 1512
FalI AAGNNNNNCTT 4 cut(s) 1968, 2000, 2154, 2186
FaqI GGGAC 3 cut(s) 131, 942, 1219
FauI CCCGC 2 cut(s) 31, 109
FauNDI CATATG 1 cut(s) 1861
FbaI TGATCA 1 cut(s) 1074
Fnu4HI GCNGC 8 cut(s) 179, 295, 1362, 1509, 1839, 2133, 2304, 2372
FokI GGATG 4 cut(s) 947, 1530, 1859, 1865
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 8 cut(s) 179, 295, 1362, 1509, 1839, 2133, 2304, 2372
FspBI CTAG 3 cut(s) 383, 1196, 2210
GlaI GCGC 2 cut(s) 73, 519
GluI GCNGC 8 cut(s) 179, 295, 1362, 1509, 1839, 2133, 2304, 2372
GsaI CCCAGC 2 cut(s) 589, 657
GsuI CTGGAG 1 cut(s) 329
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 8 cut(s) 257, 357, 400, 563, 1123, 1152, 1315, 1889
HapII CCGG 4 cut(s) 35, 285, 629, 1867
HgaI GACGC 1 cut(s) 292
HhaI GCGC 2 cut(s) 74, 520
Hin1I GRCGYC 2 cut(s) 72, 303
Hin6I GCGC 2 cut(s) 72, 518
HinP1I GCGC 2 cut(s) 72, 518
HindIII AAGCTT 1 cut(s) 1597
HinfI GANTC 6 cut(s) 281, 964, 1250, 1654, 1695, 2348
HpaII CCGG 4 cut(s) 35, 285, 629, 1867
HphI GGTGA 5 cut(s) 146, 627, 888, 1019, 2096
Hpy166II GTNNAC 4 cut(s) 1387, 1714, 1960, 2218
Hpy188III TCNNGA 7 cut(s) 285, 346, 503, 1168, 1867, 1985, 2186
Hpy8I GTNNAC 4 cut(s) 1387, 1714, 1960, 2218
Hpy99I CGWCG 2 cut(s) 302, 308
HpyAV CCTTC 7 cut(s) 225, 553, 1008, 1251, 1444, 1743, 2098
HpyCH4III ACNGT 8 cut(s) 246, 440, 572, 675, 815, 1070, 1691, 2156
HpyCH4IV ACGT 5 cut(s) 142, 335, 865, 1871, 2325
HpySE526I ACGT 5 cut(s) 142, 335, 865, 1871, 2325
Hsp92I GRCGYC 2 cut(s) 72, 303
HspAI GCGC 2 cut(s) 72, 518
KasI GGCGCC 1 cut(s) 71
Kpn2I TCCGGA 2 cut(s) 284, 1866
Ksp22I TGATCA 1 cut(s) 1074
Kzo9I GATC 4 cut(s) 505, 920, 1074, 2044
LmnI GCTCC 3 cut(s) 464, 1505, 2315
Lsp1109I GCAGC 6 cut(s) 1373, 1520, 1825, 2119, 2290, 2383
LweI GCATC 4 cut(s) 1000, 1145, 1508, 1960
MaeI CTAG 3 cut(s) 383, 1196, 2210
MaeII ACGT 5 cut(s) 142, 335, 865, 1871, 2325
MaeIII GTNAC 9 cut(s) 220, 775, 833, 1306, 1459, 1552, 1658, 1822, 1912
MalI GATC 4 cut(s) 507, 922, 1076, 2046
MbiI CCGCTC 1 cut(s) 104
MboI GATC 4 cut(s) 505, 920, 1074, 2044
MfeI CAATTG 5 cut(s) 682, 1563, 1917, 2352, 2376
MflI RGATCY 2 cut(s) 505, 920
MhlI GDGCHC 4 cut(s) 366, 447, 1052, 1411
MlsI TGGCCA 2 cut(s) 1152, 1889
MluNI TGGCCA 2 cut(s) 1152, 1889
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 3 cut(s) 1259, 1704, 2357
MmeI TCCRAC 2 cut(s) 1710, 2325
Mox20I TGGCCA 2 cut(s) 1152, 1889
Mph1103I ATGCAT 1 cut(s) 1400
MroI TCCGGA 2 cut(s) 284, 1866
MroXI GAANNNNTTC 3 cut(s) 613, 1240, 1599
MscI TGGCCA 2 cut(s) 1152, 1889
MslI CAYNNNNRTG 1 cut(s) 2239
Msp20I TGGCCA 2 cut(s) 1152, 1889
MspA1I CMGCKG 1 cut(s) 1364
MspI CCGG 4 cut(s) 35, 285, 629, 1867
MspR9I CCNGG 4 cut(s) 498, 629, 1844, 2238
MunI CAATTG 5 cut(s) 682, 1563, 1917, 2352, 2376
MvaI CCWGG 3 cut(s) 498, 1844, 2238
MvnI CGCG 1 cut(s) 297
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 1 cut(s) 629
NdeI CATATG 1 cut(s) 1861
NdeII GATC 4 cut(s) 505, 920, 1074, 2044
NlaIV GGNNCC 5 cut(s) 73, 248, 753, 1410, 2011
NmeAIII GCCGAG 1 cut(s) 333
NmuCI GTSAC 4 cut(s) 220, 1306, 1459, 1912
NsiI ATGCAT 1 cut(s) 1400
NspI RCATGY 5 cut(s) 518, 1385, 1832, 1938, 2385
OliI CACNNNNGTG 1 cut(s) 2239
PaeI GCATGC 1 cut(s) 518
PaeR7I CTCGAG 1 cut(s) 359
PagI TCATGA 1 cut(s) 2185
PceI AGGCCT 1 cut(s) 1123
PciI ACATGT 2 cut(s) 1381, 1828
PdmI GAANNNNTTC 3 cut(s) 613, 1240, 1599
PfeI GAWTC 3 cut(s) 281, 964, 1654
PflFI GACNNNGTC 1 cut(s) 302
PflMI CCANNNNNTGG 1 cut(s) 1809
PkrI GCNGC 8 cut(s) 180, 296, 1363, 1510, 1840, 2134, 2305, 2373
PleI GAGTC 3 cut(s) 1258, 1703, 2356
PluTI GGCGCC 1 cut(s) 75
PmaCI CACGTG 1 cut(s) 143
PmlI CACGTG 1 cut(s) 143
PpsI GAGTC 3 cut(s) 1258, 1703, 2356
Ppu21I YACGTR 1 cut(s) 143
PscI ACATGT 2 cut(s) 1381, 1828
PshBI ATTAAT 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 366
Psp6I CCWGG 3 cut(s) 496, 1842, 2236
PspCI CACGTG 1 cut(s) 143
PspFI CCCAGC 2 cut(s) 585, 653
PspGI CCWGG 3 cut(s) 496, 1842, 2236
PspN4I GGNNCC 5 cut(s) 73, 248, 753, 1410, 2011
PspPI GGNCC 3 cut(s) 256, 453, 1313
PspXI VCTCGAGB 1 cut(s) 359
PstI CTGCAG 1 cut(s) 2137
PsuI RGATCY 2 cut(s) 505, 920
PsyI GACNNNGTC 1 cut(s) 302
PvuII CAGCTG 1 cut(s) 1364
RsaI GTAC 3 cut(s) 574, 2143, 2219
RsaNI GTAC 3 cut(s) 573, 2142, 2218
RseI CAYNNNNRTG 1 cut(s) 2239
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 8 cut(s) 179, 295, 1362, 1509, 1839, 2133, 2304, 2372
Sau3AI GATC 4 cut(s) 505, 920, 1074, 2044
Sau96I GGNCC 3 cut(s) 256, 453, 1313
SchI GAGTC 3 cut(s) 1259, 1704, 2357
ScrFI CCNGG 4 cut(s) 498, 629, 1844, 2238
SduI GDGCHC 4 cut(s) 366, 447, 1052, 1411
SfaNI GCATC 4 cut(s) 1000, 1145, 1508, 1960
SfcI CTRYAG 2 cut(s) 814, 2133
SfoI GGCGCC 1 cut(s) 73
Sfr274I CTCGAG 1 cut(s) 359
SinI GGWCC 1 cut(s) 453
SlaI CTCGAG 1 cut(s) 359
SmiMI CAYNNNNRTG 1 cut(s) 2239
SmlI CTYRAG 2 cut(s) 359, 1166
SmoI CTYRAG 2 cut(s) 359, 1166
SpeI ACTAGT 1 cut(s) 2209
SphI GCATGC 1 cut(s) 518
SseBI AGGCCT 1 cut(s) 1123
SsiI CCGC 8 cut(s) 38, 102, 133, 176, 179, 264, 295, 1454
SspDI GGCGCC 1 cut(s) 71
SspI AATATT 1 cut(s) 90
SspMI CTAG 3 cut(s) 383, 1196, 2210
SstI GAGCTC 1 cut(s) 366
StuI AGGCCT 1 cut(s) 1123
StyD4I CCNGG 4 cut(s) 496, 627, 1842, 2236
StyI CCWWGG 3 cut(s) 382, 1332, 1512
TaaI ACNGT 8 cut(s) 246, 440, 572, 675, 815, 1070, 1691, 2156
TaiI ACGT 5 cut(s) 145, 338, 868, 1874, 2328
TaqI TCGA 3 cut(s) 360, 662, 849
TatI WGTACW 1 cut(s) 2217
TauI GCSGC 2 cut(s) 181, 297
TfiI GAWTC 3 cut(s) 281, 964, 1654
TscAI CASTG 3 cut(s) 1052, 1315, 2011
TseFI GTSAC 4 cut(s) 220, 1306, 1459, 1912
TseI GCWGC 6 cut(s) 1361, 1508, 1838, 2132, 2303, 2371
Tsp45I GTSAC 4 cut(s) 220, 1306, 1459, 1912
TspGWI ACGGA 1 cut(s) 2116
TspRI CASTG 3 cut(s) 1052, 1315, 2011
Tth111I GACNNNGTC 1 cut(s) 302
Van91I CCANNNNNTGG 1 cut(s) 1809
VpaK11BI GGWCC 1 cut(s) 453
VspI ATTAAT 1 cut(s) 11
XceI RCATGY 5 cut(s) 518, 1385, 1832, 1938, 2385
XhoI CTCGAG 1 cut(s) 359
XmaJI CCTAGG 1 cut(s) 382
XmnI GAANNNNTTC 3 cut(s) 613, 1240, 1599
XspI CTAG 3 cut(s) 383, 1196, 2210
Zsp2I ATGCAT 1 cut(s) 1400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.