Rmu_sc0005365.1_g000003

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005365.1
OG0013649
Physical Location & Seq
Forward (+)
11480 .. 12060
581 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 471 bp
atgggtctgaaattggtgaatgtcctggcaatacttgtgctctcattgttagcgtctacaactacatcccaagccgatgtgtttgatgtgactagttcaatctttggtggaaagcccggctctgatattacagtgggtttggcaaaggcatggagagatgcatgtgcatcagagtcgtcaagtaaggttgttgttccaagcggttcgtacaagttgaaagaagcaacattcgaaggtccttgcaaggctccgattgaggttaagatccaaggaacactgcaggctccaaaagaccgaagttcagatacttgggttgcatttagctccattgacatgctcaccttatggggtggtggaacttttgacggccaaggagcacatggttcgaaagacggtgatatcagagatatcaggttctcctccatcaccaattccataattatggacataacttcactagatagtaagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

156

Amino Acids

16.39

Weight (kDa)

5.3

Isoelectric Point (pI)

32.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 55 - 127 1.5e-12 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 56
AciI CCGC 1 cut(s) 201
AclWI GGATC 1 cut(s) 259
AcoI YGGCCR 1 cut(s) 367
AfaI GTAC 1 cut(s) 209
AgsI TTSAA 2 cut(s) 99, 217
AhlI ACTAGT 1 cut(s) 92
AjnI CCWGG 1 cut(s) 24
AluBI AGCT 1 cut(s) 324
AluI AGCT 1 cut(s) 324
Alw21I GWGCWC 2 cut(s) 42, 379
AlwI GGATC 1 cut(s) 259
AoxI GGCC 1 cut(s) 367
AspS9I GGNCC 1 cut(s) 236
AsuC2I CCSGG 1 cut(s) 117
AsuHPI GGTGA 4 cut(s) 28, 331, 407, 418
AsuII TTCGAA 2 cut(s) 231, 386
AvaII GGWCC 1 cut(s) 236
BaeI ACNNNNGTAYC 2 cut(s) 297, 330
BarI GAAGNNNNNNTAC 2 cut(s) 289, 321
Bbv12I GWGCWC 2 cut(s) 42, 379
BccI CCATC 1 cut(s) 431
BceAI ACGGC 1 cut(s) 382
BcgI CGANNNNNNTGC 4 cut(s) 156, 190, 366, 400
BciT130I CCWGG 1 cut(s) 26
BcnI CCSGG 1 cut(s) 117
BcuI ACTAGT 1 cut(s) 92
BfaI CTAG 2 cut(s) 93, 458
BfmI CTRYAG 1 cut(s) 278
Bme1390I CCNGG 2 cut(s) 26, 117
Bme18I GGWCC 1 cut(s) 236
BmgT120I GGNCC 1 cut(s) 236
BmiI GGNNCC 2 cut(s) 249, 285
BmrFI CCNGG 2 cut(s) 26, 117
BmsI GCATC 2 cut(s) 148, 176
Bpu14I TTCGAA 2 cut(s) 231, 386
BpuMI CCSGG 1 cut(s) 117
BsaJI CCNNGG 2 cut(s) 268, 370
BseBI CCWGG 1 cut(s) 26
BseDI CCNNGG 2 cut(s) 268, 370
BseGI GGATG 1 cut(s) 65
BseRI GAGGAG 1 cut(s) 409
BshFI GGCC 1 cut(s) 369
BsiHKAI GWGCWC 2 cut(s) 42, 379
BsiSI CCGG 1 cut(s) 117
BsnI GGCC 1 cut(s) 369
Bsp119I TTCGAA 2 cut(s) 231, 386
Bsp1286I GDGCHC 2 cut(s) 42, 379
Bsp143I GATC 1 cut(s) 264
BspACI CCGC 1 cut(s) 201
BspANI GGCC 1 cut(s) 369
BspLI GGNNCC 2 cut(s) 249, 285
BspMAI CTGCAG 1 cut(s) 282
BspPI GGATC 1 cut(s) 259
BspT104I TTCGAA 2 cut(s) 231, 386
BssECI CCNNGG 2 cut(s) 268, 370
BssMI GATC 1 cut(s) 264
BssT1I CCWWGG 2 cut(s) 268, 370
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 2 cut(s) 133, 395
BstBI TTCGAA 2 cut(s) 231, 386
BstC8I GCNNGC 1 cut(s) 282
BstF5I GGATG 1 cut(s) 65
BstKTI GATC 1 cut(s) 267
BstMBI GATC 1 cut(s) 264
BstNI CCWGG 1 cut(s) 26
BstNSI RCATGY 2 cut(s) 165, 337
BstSCI CCNGG 2 cut(s) 24, 115
BstSFI CTRYAG 1 cut(s) 278
BstX2I RGATCY 1 cut(s) 264
BstXI CCANNNNNNTGG 1 cut(s) 442
BstYI RGATCY 1 cut(s) 264
BsuRI GGCC 1 cut(s) 369
BtsCI GGATG 1 cut(s) 65
BtsI GCAGTG 1 cut(s) 275
BtsIMutI CAGTG 2 cut(s) 138, 275
Cac8I GCNNGC 1 cut(s) 282
Cfr13I GGNCC 1 cut(s) 236
CseI GACGC 1 cut(s) 42
Csp6I GTAC 1 cut(s) 208
CviAII CATG 4 cut(s) 150, 162, 334, 380
CviJI RGCY 7 cut(s) 74, 115, 120, 248, 284, 324, 369
CviKI_1 RGCY 7 cut(s) 74, 115, 120, 248, 284, 324, 369
CviQI GTAC 1 cut(s) 208
DpnI GATC 1 cut(s) 266
DpnII GATC 1 cut(s) 264
EaeI YGGCCR 1 cut(s) 367
Eco130I CCWWGG 2 cut(s) 268, 370
Eco32I GATATC 2 cut(s) 400, 409
Eco47I GGWCC 1 cut(s) 236
EcoO109I RGGNCCY 1 cut(s) 236
EcoRII CCWGG 1 cut(s) 24
EcoRV GATATC 2 cut(s) 400, 409
EcoT14I CCWWGG 2 cut(s) 268, 370
EcoT22I ATGCAT 1 cut(s) 163
ErhI CCWWGG 2 cut(s) 268, 370
FaeI CATG 4 cut(s) 153, 165, 337, 383
FaiI YATR 8 cut(s) 151, 163, 335, 346, 381, 437, 443, 449
FatI CATG 4 cut(s) 149, 161, 333, 379
FblI GTMKAC 1 cut(s) 56
FokI GGATG 1 cut(s) 52
FspBI CTAG 2 cut(s) 93, 458
HaeIII GGCC 1 cut(s) 369
HapII CCGG 1 cut(s) 117
HgaI GACGC 1 cut(s) 42
Hin1II CATG 4 cut(s) 153, 165, 337, 383
HinfI GANTC 1 cut(s) 173
HpaII CCGG 1 cut(s) 117
HphI GGTGA 4 cut(s) 28, 331, 407, 418
Hpy166II GTNNAC 1 cut(s) 57
Hpy188I TCNGA 6 cut(s) 9, 124, 172, 252, 304, 404
Hpy8I GTNNAC 1 cut(s) 57
HpyAV CCTTC 1 cut(s) 227
HpyCH4III ACNGT 2 cut(s) 133, 395
HpyCH4V TGCA 5 cut(s) 161, 167, 243, 280, 317
Hsp92II CATG 4 cut(s) 153, 165, 337, 383
Kzo9I GATC 1 cut(s) 264
LmnI GCTCC 4 cut(s) 253, 289, 329, 374
LpnPI CCDG 5 cut(s) 11, 38, 130, 266, 397
LweI GCATC 2 cut(s) 148, 176
MaeI CTAG 2 cut(s) 93, 458
MaeIII GTNAC 1 cut(s) 88
MalI GATC 1 cut(s) 266
MboI GATC 1 cut(s) 264
MflI RGATCY 1 cut(s) 264
MhlI GDGCHC 2 cut(s) 42, 379
MluCI AATT 3 cut(s) 11, 430, 438
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 2 cut(s) 250, 430
Mph1103I ATGCAT 1 cut(s) 163
MseI TTAA 1 cut(s) 261
MslI CAYNNNNRTG 2 cut(s) 332, 440
MspI CCGG 1 cut(s) 117
MspR9I CCNGG 2 cut(s) 26, 117
MvaI CCWGG 1 cut(s) 26
NciI CCSGG 1 cut(s) 117
NdeII GATC 1 cut(s) 264
NlaIII CATG 4 cut(s) 153, 165, 337, 383
NlaIV GGNNCC 2 cut(s) 249, 285
NmuCI GTSAC 1 cut(s) 88
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 2 cut(s) 165, 337
NspV TTCGAA 2 cut(s) 231, 386
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
PpuMI RGGWCCY 1 cut(s) 236
Psp5II RGGWCCY 1 cut(s) 236
Psp6I CCWGG 1 cut(s) 24
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 2 cut(s) 249, 285
PspPI GGNCC 1 cut(s) 236
PspPPI RGGWCCY 1 cut(s) 236
PstI CTGCAG 1 cut(s) 282
PsuI RGATCY 1 cut(s) 264
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 2 cut(s) 332, 440
SaqAI TTAA 1 cut(s) 261
Sau3AI GATC 1 cut(s) 264
Sau96I GGNCC 1 cut(s) 236
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 26, 117
SduI GDGCHC 2 cut(s) 42, 379
SetI ASST 6 cut(s) 189, 238, 261, 326, 344, 416
SfaNI GCATC 2 cut(s) 148, 176
SfcI CTRYAG 1 cut(s) 278
SfuI TTCGAA 2 cut(s) 231, 386
SinI GGWCC 1 cut(s) 236
SmiMI CAYNNNNRTG 2 cut(s) 332, 440
SpeI ACTAGT 1 cut(s) 92
Sse9I AATT 3 cut(s) 11, 430, 438
SsiI CCGC 1 cut(s) 201
SspMI CTAG 2 cut(s) 93, 458
StyD4I CCNGG 2 cut(s) 24, 115
StyI CCWWGG 2 cut(s) 268, 370
TaaI ACNGT 2 cut(s) 133, 395
TaqI TCGA 2 cut(s) 231, 386
TaqII GACCGA 1 cut(s) 309
TasI AATT 3 cut(s) 11, 430, 438
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 2 cut(s) 138, 282
TseFI GTSAC 1 cut(s) 88
Tsp45I GTSAC 1 cut(s) 88
TspRI CASTG 2 cut(s) 138, 282
VpaK11BI GGWCC 1 cut(s) 236
XceI RCATGY 2 cut(s) 165, 337
XcmI CCANNNNNNNNNTGG 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 56
XspI CTAG 2 cut(s) 93, 458
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.