Rmu_sc0005498.1_g000005

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005498.1
OG0012553
Physical Location & Seq
Reverse (-)
14817 .. 17006
2190 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1137 bp
atgggaaacatggagaacaataacatcaataccaacaacaacaacaacaacaacaacaacagcaagcagcagtctttgcattgtgaaaacgagaagggtaatttggagaaggaatctccggtgatgagctccgatcaacagagcaccgtgttgaattcttttcagcttgaaagcatatctgaggattcagtagctgtagaccgaaaacagaacatgtcacattttgtccctgcccttcgatcaggagagtggtctgatataggagatcgtccccatatggaagatactcatatatgcatcagtgacttggctaagaagtttggttataatgtactcaatgatgaagctatctccttctatggtgtatttgatggtcatgggggaaaggatgcagcgcaatttgttcgtgacaacctgccaagagtcatcgttgaggatgctgacttccctgtagagcttgaaaaggtgataacaaggtcatttgttgagacagatgctgcatttgcaaaaacttgctcacttcagtcttcccttgcttctggcacaactgcactcactgcaatgatatttgggaggtctttgcttgtggcgaatgctggagattgcagggctatattgtcgcgaaatggagcagctatagaaatgtcaaaagatcatagaccctgctgcacaaaagaaaggatgcgaattgagtctttgggtggatatgttgatgatggttatctgaatggtcagttaggggtcacccgtgcattaggtgattggcatcttgaaggattgaaggatgagggtgaaaggggaggacccttgagtgctgaaccggaacttaaattgacaacactgaccaaagatgatgaatttttgatcattggtagtgatggaatatgggatgtgttcaccaaccaaaatgctgtagactttgctcgaaggaaactccaagagcgcaatgatgtgaagctgtgttgcaagcaaatagtagaggaggcaataaaacgaggcgcaaccgacaatttgacacttgttatggtgagtttccacttggagccacctccacatctggtggtagaaaggtctagagtcagacgtagtatttctgctgagggactacaaaatcttcagtccttcttagatcgatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

378

Amino Acids

41.93

Weight (kDa)

5.07

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 91 - 337 3.5e-68 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
Acc36I ACCTGC 1 cut(s) 423
AccI GTMKAC 2 cut(s) 198, 915
AccII CGCG 1 cut(s) 622
AcsI RAATTY 2 cut(s) 154, 857
AcuI CTGAAG 2 cut(s) 506, 1100
AfaI GTAC 1 cut(s) 333
AflIII ACRYGT 1 cut(s) 213
AgsI TTSAA 5 cut(s) 154, 170, 461, 773, 781
AjuI GAANNNNNNNTTGG 2 cut(s) 86, 118
AluBI AGCT 7 cut(s) 129, 166, 194, 347, 457, 635, 958
AluI AGCT 7 cut(s) 129, 166, 194, 347, 457, 635, 958
Alw21I GWGCWC 2 cut(s) 131, 146
Alw26I GTCTC 1 cut(s) 482
AlwNI CAGNNNCTG 2 cut(s) 194, 497
ApeKI GCWGC 5 cut(s) 67, 392, 497, 632, 666
ApoI RAATTY 2 cut(s) 154, 857
AspLEI GCGC 3 cut(s) 397, 945, 1001
AspS9I GGNCC 1 cut(s) 803
AsuHPI GGTGA 7 cut(s) 133, 478, 736, 770, 803, 889, 1039
AvaII GGWCC 1 cut(s) 803
BanII GRGCYC 1 cut(s) 131
BbsI GAAGAC 1 cut(s) 519
Bbv12I GWGCWC 2 cut(s) 131, 146
BbvCI CCTCAGC 1 cut(s) 1098
BbvI GCAGC 5 cut(s) 79, 404, 484, 644, 653
BccI CCATC 3 cut(s) 365, 710, 872
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BclI TGATCA 1 cut(s) 864
BcoDI GTCTC 1 cut(s) 482
BfaI CTAG 1 cut(s) 1074
BfmI CTRYAG 4 cut(s) 195, 450, 636, 912
BfuAI ACCTGC 1 cut(s) 423
BisI GCNGC 5 cut(s) 68, 393, 498, 633, 667
BlsI GCNGC 5 cut(s) 69, 394, 499, 634, 668
Bme18I GGWCC 1 cut(s) 803
BmgT120I GGNCC 1 cut(s) 803
BmiI GGNNCC 2 cut(s) 805, 1044
BmsI GCATC 6 cut(s) 306, 379, 427, 484, 672, 775
BpiI GAAGAC 1 cut(s) 519
BpmI CTGGAG 1 cut(s) 618
Bpu10I CCTNAGC 1 cut(s) 1098
BpuEI CTTGAG 1 cut(s) 829
Bsa29I ATCGAT 1 cut(s) 1132
BsaBI GATNNNNATC 2 cut(s) 720, 765
BsaWI WCCGGW 2 cut(s) 118, 820
Bse3DI GCAATG 2 cut(s) 567, 952
Bse8I GATNNNNATC 2 cut(s) 720, 765
BseCI ATCGAT 1 cut(s) 1132
BseGI GGATG 5 cut(s) 394, 442, 687, 790, 895
BseJI GATNNNNATC 2 cut(s) 720, 765
BseMI GCAATG 2 cut(s) 567, 952
BseMII CTCAG 2 cut(s) 171, 1089
BseRI GAGGAG 1 cut(s) 995
BseXI GCAGC 5 cut(s) 79, 404, 484, 644, 653
BsgI GTGCAG 2 cut(s) 534, 652
Bsh1236I CGCG 1 cut(s) 622
BshVI ATCGAT 1 cut(s) 1132
BsiHKAI GWGCWC 2 cut(s) 131, 146
BsiSI CCGG 2 cut(s) 119, 821
BslFI GGGAC 3 cut(s) 212, 255, 1116
BsmAI GTCTC 1 cut(s) 482
BsmFI GGGAC 3 cut(s) 212, 255, 1116
BsmI GAATGC 1 cut(s) 598
Bsp1286I GDGCHC 2 cut(s) 131, 146
Bsp143I GATC 6 cut(s) 133, 239, 265, 652, 864, 1129
Bsp68I TCGCGA 1 cut(s) 622
BspCNI CTCAG 2 cut(s) 172, 1090
BspDI ATCGAT 1 cut(s) 1132
BspFNI CGCG 1 cut(s) 622
BspLI GGNNCC 2 cut(s) 805, 1044
BspMI ACCTGC 1 cut(s) 423
BsrDI GCAATG 2 cut(s) 567, 952
BssMI GATC 6 cut(s) 133, 239, 265, 652, 864, 1129
Bst4CI ACNGT 1 cut(s) 148
BstAPI GCANNNNNTGC 2 cut(s) 76, 557
BstC8I GCNNGC 2 cut(s) 65, 968
BstDEI CTNAG 4 cut(s) 180, 312, 1098, 1126
BstEII GGTNACC 1 cut(s) 742
BstF5I GGATG 5 cut(s) 394, 442, 687, 790, 895
BstFNI CGCG 1 cut(s) 622
BstHHI GCGC 3 cut(s) 397, 945, 1001
BstKTI GATC 6 cut(s) 136, 242, 268, 655, 867, 1132
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 6 cut(s) 133, 239, 265, 652, 864, 1129
BstMWI GCNNNNNNNGC 3 cut(s) 76, 503, 557
BstNSI RCATGY 1 cut(s) 217
BstPI GGTNACC 1 cut(s) 742
BstSFI CTRYAG 4 cut(s) 195, 450, 636, 912
BstUI CGCG 1 cut(s) 622
BstV1I GCAGC 5 cut(s) 79, 404, 484, 644, 653
BstV2I GAAGAC 1 cut(s) 519
Bsu15I ATCGAT 1 cut(s) 1132
BsuTUI ATCGAT 1 cut(s) 1132
BtsCI GGATG 5 cut(s) 394, 442, 687, 790, 895
BtsI GCAGTG 1 cut(s) 555
BtsIMutI CAGTG 3 cut(s) 307, 555, 839
BtuMI TCGCGA 1 cut(s) 622
BveI ACCTGC 1 cut(s) 423
Cac8I GCNNGC 2 cut(s) 65, 968
CaiI CAGNNNCTG 2 cut(s) 194, 497
CfoI GCGC 3 cut(s) 397, 945, 1001
Cfr13I GGNCC 1 cut(s) 803
ClaI ATCGAT 1 cut(s) 1132
Csp6I GTAC 1 cut(s) 332
CviAII CATG 3 cut(s) 10, 214, 377
CviQI GTAC 1 cut(s) 332
DdeI CTNAG 4 cut(s) 180, 312, 1098, 1126
DpnI GATC 6 cut(s) 135, 241, 267, 654, 866, 1131
DpnII GATC 6 cut(s) 133, 239, 265, 652, 864, 1129
Ecl136II GAGCTC 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 131
Eco47I GGWCC 1 cut(s) 803
Eco53kI GAGCTC 1 cut(s) 129
Eco57I CTGAAG 2 cut(s) 506, 1100
Eco91I GGTNACC 1 cut(s) 742
EcoICRI GAGCTC 1 cut(s) 129
EcoO109I RGGNCCY 1 cut(s) 803
EcoO65I GGTNACC 1 cut(s) 742
EcoRI GAATTC 1 cut(s) 154
EcoT22I ATGCAT 1 cut(s) 299
EcoT38I GRGCYC 1 cut(s) 131
FaeI CATG 3 cut(s) 13, 217, 380
FaqI GGGAC 3 cut(s) 212, 255, 1116
FatI CATG 3 cut(s) 9, 213, 376
FauNDI CATATG 1 cut(s) 276
FbaI TGATCA 1 cut(s) 864
FblI GTMKAC 2 cut(s) 198, 915
Fnu4HI GCNGC 5 cut(s) 68, 393, 498, 633, 667
FokI GGATG 5 cut(s) 401, 449, 694, 797, 902
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 5 cut(s) 68, 393, 498, 633, 667
FspBI CTAG 1 cut(s) 1074
GlaI GCGC 3 cut(s) 396, 944, 1000
GluI GCNGC 5 cut(s) 68, 393, 498, 633, 667
GsuI CTGGAG 1 cut(s) 618
HapII CCGG 2 cut(s) 119, 821
HhaI GCGC 3 cut(s) 397, 945, 1001
Hin1II CATG 3 cut(s) 13, 217, 380
Hin6I GCGC 3 cut(s) 395, 943, 999
HinP1I GCGC 3 cut(s) 395, 943, 999
HinfI GANTC 5 cut(s) 113, 185, 423, 692, 1077
HpaII CCGG 2 cut(s) 119, 821
HphI GGTGA 7 cut(s) 133, 478, 736, 770, 803, 889, 1039
Hpy166II GTNNAC 3 cut(s) 199, 897, 916
Hpy188I TCNGA 5 cut(s) 133, 181, 256, 726, 1082
Hpy188III TCNNGA 5 cut(s) 243, 407, 621, 770, 1074
Hpy8I GTNNAC 3 cut(s) 199, 897, 916
HpyAV CCTTC 8 cut(s) 88, 103, 245, 364, 767, 775, 921, 1132
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 1 cut(s) 1084
HpyF10VI GCNNNNNNNGC 3 cut(s) 76, 503, 557
HpyF3I CTNAG 4 cut(s) 180, 312, 1098, 1126
HpySE526I ACGT 1 cut(s) 1084
Hsp92II CATG 3 cut(s) 13, 217, 380
HspAI GCGC 3 cut(s) 395, 943, 999
Ksp22I TGATCA 1 cut(s) 864
Kzo9I GATC 6 cut(s) 133, 239, 265, 652, 864, 1129
LmnI GCTCC 3 cut(s) 134, 629, 1042
Lsp1109I GCAGC 5 cut(s) 79, 404, 484, 644, 653
LweI GCATC 6 cut(s) 306, 379, 427, 484, 672, 775
MaeI CTAG 1 cut(s) 1074
MaeII ACGT 1 cut(s) 1084
MaeIII GTNAC 4 cut(s) 216, 302, 407, 742
MalI GATC 6 cut(s) 135, 241, 267, 654, 866, 1131
MboI GATC 6 cut(s) 133, 239, 265, 652, 864, 1129
MboII GAAGA 3 cut(s) 293, 519, 1106
MhlI GDGCHC 2 cut(s) 131, 146
MluCI AATT 7 cut(s) 100, 154, 398, 687, 830, 857, 1009
MlyI GAGTC 3 cut(s) 432, 701, 1086
Mph1103I ATGCAT 1 cut(s) 299
MseI TTAA 1 cut(s) 828
MslI CAYNNNNRTG 1 cut(s) 560
MspI CCGG 2 cut(s) 119, 821
Mva1269I GAATGC 1 cut(s) 598
MvnI CGCG 1 cut(s) 622
MwoI GCNNNNNNNGC 3 cut(s) 76, 503, 557
NdeI CATATG 1 cut(s) 276
NdeII GATC 6 cut(s) 133, 239, 265, 652, 864, 1129
NlaIII CATG 3 cut(s) 13, 217, 380
NlaIV GGNNCC 2 cut(s) 805, 1044
NmuCI GTSAC 4 cut(s) 216, 302, 407, 742
NruI TCGCGA 1 cut(s) 622
NsiI ATGCAT 1 cut(s) 299
NspI RCATGY 1 cut(s) 217
PciI ACATGT 1 cut(s) 213
PctI GAATGC 1 cut(s) 598
PfeI GAWTC 2 cut(s) 113, 185
PkrI GCNGC 5 cut(s) 69, 394, 499, 634, 668
PleI GAGTC 3 cut(s) 431, 700, 1085
PpsI GAGTC 3 cut(s) 431, 700, 1085
PpuMI RGGWCCY 1 cut(s) 803
PscI ACATGT 1 cut(s) 213
PsiI TTATAA 1 cut(s) 327
Psp124BI GAGCTC 1 cut(s) 131
Psp5II RGGWCCY 1 cut(s) 803
PspEI GGTNACC 1 cut(s) 742
PspN4I GGNNCC 2 cut(s) 805, 1044
PspPI GGNCC 1 cut(s) 803
PspPPI RGGWCCY 1 cut(s) 803
PstNI CAGNNNCTG 2 cut(s) 194, 497
RruI TCGCGA 1 cut(s) 622
RsaI GTAC 1 cut(s) 333
RsaNI GTAC 1 cut(s) 332
RseI CAYNNNNRTG 1 cut(s) 560
SacI GAGCTC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 828
SatI GCNGC 5 cut(s) 68, 393, 498, 633, 667
Sau3AI GATC 6 cut(s) 133, 239, 265, 652, 864, 1129
Sau96I GGNCC 1 cut(s) 803
SchI GAGTC 3 cut(s) 432, 701, 1086
SduI GDGCHC 2 cut(s) 131, 146
SfaNI GCATC 6 cut(s) 306, 379, 427, 484, 672, 775
SfcI CTRYAG 4 cut(s) 195, 450, 636, 912
SinI GGWCC 1 cut(s) 803
SmiMI CAYNNNNRTG 1 cut(s) 560
SmlI CTYRAG 1 cut(s) 808
SmoI CTYRAG 1 cut(s) 808
Sse9I AATT 7 cut(s) 100, 154, 398, 687, 830, 857, 1009
SspMI CTAG 1 cut(s) 1074
SstI GAGCTC 1 cut(s) 131
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 1 cut(s) 1087
TaqI TCGA 3 cut(s) 238, 925, 1132
TaqII GACCGA 1 cut(s) 216
TasI AATT 7 cut(s) 100, 154, 398, 687, 830, 857, 1009
TatI WGTACW 1 cut(s) 331
TfiI GAWTC 2 cut(s) 113, 185
Tru1I TTAA 1 cut(s) 828
Tru9I TTAA 1 cut(s) 828
TscAI CASTG 3 cut(s) 307, 562, 846
TseFI GTSAC 4 cut(s) 216, 302, 407, 742
TseI GCWGC 5 cut(s) 67, 392, 497, 632, 666
Tsp45I GTSAC 4 cut(s) 216, 302, 407, 742
TspDTI ATGAA 2 cut(s) 357, 870
TspRI CASTG 3 cut(s) 307, 562, 846
VpaK11BI GGWCC 1 cut(s) 803
XapI RAATTY 2 cut(s) 154, 857
XbaI TCTAGA 1 cut(s) 1073
XceI RCATGY 1 cut(s) 217
XmiI GTMKAC 2 cut(s) 198, 915
XspI CTAG 1 cut(s) 1074
Zsp2I ATGCAT 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.