Rmu_sc0005899.1_g000006

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005899.1
OG0007471
Physical Location & Seq
Reverse (-)
20563 .. 22376
1814 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 846 bp
atgcggagaaaatatgatcttttagaggtggttttcaagttcaagatcgggcctcaggtcaatgcctcgggcttcgttcccatgaccttgctagtggtttgggtttcttctcgcggatggtgtctccggttgccctatgatattaaggtcaagaaaacatcggtcctctggcatgaccaccggaaaaacgctagaaaccccaaaccaaaagcagtcgcagatcttttcatctccgctatggcagtctccgttcccatagtggccatcatcacctctctccacctcatcgccttcattttcgccgtcggcgccgaacgacgccgtagcaccgcgaagatagtgcccgacgagtacgacgagcaaacctactgcgtttacggcaccgacgcttccaccgtgtacggactggccgcgttcgggcttcttctgctcagccacacggtgcttaacggcgtcaccaggtgtctctgcttcggcaaaggcttaatcaccggctcctctactacttggactgtcttcttcttcgtcttctcctggacaacctttttgggagccgaggcatgcttattggcagggtcagcaaggaatgcctatcacacaaagtacagggggaaatttaacctagacgacttgtcctgtgccactctccgtaagggcgtgtttgctgcagcagctgctcttacactgttgtcgctgatagggtcaaacctttactactggacacattccaaagctgatactggagggtgggagaagcaccgcaatgaaggccttggcatgggcaattccaattatgggcagcacgagcagcagctgcagcaaaccactggattcgagaaggtgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

281

Amino Acids

31.18

Weight (kDa)

9.48

Isoelectric Point (pI)

25.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 138 - 232 4.3e-26 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 308, 380
AccII CGCG 3 cut(s) 114, 332, 413
AciI CCGC 6 cut(s) 4, 114, 234, 330, 411, 760
AcoI YGGCCR 2 cut(s) 261, 408
AcsI RAATTY 1 cut(s) 614
AcyI GRCGYC 3 cut(s) 309, 319, 453
AdeI CACNNNGTG 2 cut(s) 442, 462
AfaI GTAC 3 cut(s) 353, 401, 605
AfiI CCNNNNNNNGG 3 cut(s) 417, 778, 795
AgsI TTSAA 2 cut(s) 37, 43
AhdI GACNNNNNGTC 1 cut(s) 631
AjnI CCWGG 2 cut(s) 458, 533
AluBI AGCT 3 cut(s) 674, 734, 814
AluI AGCT 3 cut(s) 674, 734, 814
Alw26I GTCTC 3 cut(s) 128, 250, 470
AlwNI CAGNNNCTG 2 cut(s) 674, 814
Ama87I CYCGRG 1 cut(s) 67
AoxI GGCC 4 cut(s) 50, 261, 408, 769
ApeKI GCWGC 9 cut(s) 665, 668, 671, 674, 799, 808, 811, 814, 817
ApoI RAATTY 1 cut(s) 614
ArsI GACNNNNNNTTYG 2 cut(s) 529, 561
AspLEI GCGC 1 cut(s) 311
AspS9I GGNCC 2 cut(s) 50, 163
AsuHPI GGTGA 3 cut(s) 262, 448, 481
AvaI CYCGRG 1 cut(s) 67
AvaII GGWCC 1 cut(s) 163
AxyI CCTNAGG 1 cut(s) 54
BaeGI GKGCMC 1 cut(s) 345
BalI TGGCCA 1 cut(s) 263
BanI GGYRCC 2 cut(s) 308, 380
BauI CACGAG 1 cut(s) 803
BbsI GAAGAC 2 cut(s) 508, 520
BbvI GCAGC 9 cut(s) 652, 661, 680, 683, 801, 811, 820, 823, 829
BccI CCATC 2 cut(s) 111, 272
BceAI ACGGC 4 cut(s) 287, 306, 394, 466
BcgI CGANNNNNNTGC 2 cut(s) 322, 356
BciT130I CCWGG 2 cut(s) 460, 535
BcoDI GTCTC 3 cut(s) 128, 250, 470
BfaI CTAG 3 cut(s) 92, 192, 623
BfmI CTRYAG 2 cut(s) 666, 815
BfoI RGCGCY 1 cut(s) 312
BglII AGATCT 1 cut(s) 220
BlpI GCTNAGC 1 cut(s) 431
Bme1390I CCNGG 2 cut(s) 460, 535
Bme18I GGWCC 1 cut(s) 163
BmeRI GACNNNNNGTC 1 cut(s) 631
BmeT110I CYCGRG 1 cut(s) 67
BmgT120I GGNCC 2 cut(s) 50, 163
BmiI GGNNCC 4 cut(s) 310, 382, 496, 553
BmrFI CCNGG 2 cut(s) 460, 535
BpiI GAAGAC 2 cut(s) 508, 520
BpmI CTGGAG 1 cut(s) 762
Bpu1102I GCTNAGC 1 cut(s) 431
BsaHI GRCGYC 3 cut(s) 309, 319, 453
BsaJI CCNNGG 3 cut(s) 66, 555, 772
BsaWI WCCGGW 2 cut(s) 126, 180
Bsc4I CCNNNNNNNGG 3 cut(s) 417, 778, 795
Bse118I RCCGGY 1 cut(s) 491
Bse1I ACTGG 4 cut(s) 411, 722, 745, 832
Bse21I CCTNAGG 1 cut(s) 54
Bse3DI GCAATG 1 cut(s) 769
BseBI CCWGG 2 cut(s) 460, 535
BseDI CCNNGG 3 cut(s) 66, 555, 772
BseGI GGATG 1 cut(s) 122
BseLI CCNNNNNNNGG 3 cut(s) 417, 778, 795
BseMI GCAATG 1 cut(s) 769
BseMII CTCAG 2 cut(s) 68, 445
BseNI ACTGG 4 cut(s) 411, 722, 745, 832
BseRI GAGGAG 1 cut(s) 487
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 9 cut(s) 652, 661, 680, 683, 801, 811, 820, 823, 829
Bsh1236I CGCG 3 cut(s) 114, 332, 413
BshFI GGCC 4 cut(s) 52, 263, 410, 771
BshNI GGYRCC 2 cut(s) 308, 380
BsiHKCI CYCGRG 1 cut(s) 67
BsiSI CCGG 3 cut(s) 127, 181, 492
BslI CCNNNNNNNGG 3 cut(s) 417, 778, 795
BsmAI GTCTC 3 cut(s) 128, 250, 470
BsmI GAATGC 1 cut(s) 592
BsnI GGCC 4 cut(s) 52, 263, 410, 771
BsoBI CYCGRG 1 cut(s) 67
Bsp1286I GDGCHC 1 cut(s) 345
Bsp143I GATC 3 cut(s) 16, 45, 220
Bsp1720I GCTNAGC 1 cut(s) 431
BspACI CCGC 6 cut(s) 4, 114, 234, 330, 411, 760
BspANI GGCC 4 cut(s) 52, 263, 410, 771
BspCNI CTCAG 2 cut(s) 67, 444
BspFNI CGCG 3 cut(s) 114, 332, 413
BspLI GGNNCC 4 cut(s) 310, 382, 496, 553
BspMAI CTGCAG 2 cut(s) 670, 819
BspT107I GGYRCC 2 cut(s) 308, 380
BsrDI GCAATG 1 cut(s) 769
BsrFI RCCGGY 1 cut(s) 491
BsrI ACTGG 4 cut(s) 411, 722, 745, 832
BssAI RCCGGY 1 cut(s) 491
BssECI CCNNGG 3 cut(s) 66, 555, 772
BssMI GATC 3 cut(s) 16, 45, 220
BssNI GRCGYC 3 cut(s) 309, 319, 453
BssSI CACGAG 1 cut(s) 803
BssT1I CCWWGG 1 cut(s) 772
Bst2BI CACGAG 1 cut(s) 803
Bst2UI CCWGG 2 cut(s) 460, 535
Bst4CI ACNGT 4 cut(s) 397, 442, 514, 687
BstACI GRCGYC 3 cut(s) 309, 319, 453
BstAPI GCANNNNNTGC 3 cut(s) 587, 674, 814
BstC8I GCNNGC 1 cut(s) 562
BstDEI CTNAG 2 cut(s) 54, 431
BstF5I GGATG 1 cut(s) 122
BstFNI CGCG 3 cut(s) 114, 332, 413
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 3 cut(s) 19, 48, 223
BstMAI GTCTC 3 cut(s) 128, 250, 470
BstMBI GATC 3 cut(s) 16, 45, 220
BstNI CCWGG 2 cut(s) 460, 535
BstNSI RCATGY 1 cut(s) 564
BstSCI CCNGG 2 cut(s) 458, 533
BstSFI CTRYAG 2 cut(s) 666, 815
BstSLI GKGCMC 1 cut(s) 345
BstUI CGCG 3 cut(s) 114, 332, 413
BstV1I GCAGC 9 cut(s) 652, 661, 680, 683, 801, 811, 820, 823, 829
BstV2I GAAGAC 2 cut(s) 508, 520
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
Bsu36I CCTNAGG 1 cut(s) 54
BsuRI GGCC 4 cut(s) 52, 263, 410, 771
BtgZI GCGATG 1 cut(s) 271
BtsCI GGATG 1 cut(s) 122
BtsIMutI CAGTG 2 cut(s) 683, 825
Cac8I GCNNGC 1 cut(s) 562
CaiI CAGNNNCTG 2 cut(s) 674, 814
CfoI GCGC 1 cut(s) 311
Cfr10I RCCGGY 1 cut(s) 491
Cfr13I GGNCC 2 cut(s) 50, 163
CseI GACGC 3 cut(s) 327, 395, 442
CsiI ACCWGGT 1 cut(s) 458
Csp6I GTAC 3 cut(s) 352, 400, 604
CviAII CATG 4 cut(s) 82, 173, 561, 778
CviQI GTAC 3 cut(s) 352, 400, 604
DdeI CTNAG 2 cut(s) 54, 431
DinI GGCGCC 1 cut(s) 310
DpnI GATC 3 cut(s) 18, 47, 222
DpnII GATC 3 cut(s) 16, 45, 220
DraIII CACNNNGTG 2 cut(s) 442, 462
DriI GACNNNNNGTC 1 cut(s) 631
EaeI YGGCCR 2 cut(s) 261, 408
Eam1105I GACNNNNNGTC 1 cut(s) 631
Eco130I CCWWGG 1 cut(s) 772
Eco147I AGGCCT 1 cut(s) 771
Eco47I GGWCC 1 cut(s) 163
Eco81I CCTNAGG 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 67
EcoRII CCWGG 2 cut(s) 458, 533
EcoT14I CCWWGG 1 cut(s) 772
EgeI GGCGCC 1 cut(s) 310
EheI GGCGCC 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 772
FaeI CATG 4 cut(s) 85, 176, 564, 781
FaiI YATR 9 cut(s) 15, 83, 138, 174, 239, 257, 562, 779, 795
FatI CATG 4 cut(s) 81, 172, 560, 777
FokI GGATG 1 cut(s) 129
FspBI CTAG 3 cut(s) 92, 192, 623
GlaI GCGC 1 cut(s) 310
GsuI CTGGAG 1 cut(s) 762
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 4 cut(s) 52, 263, 410, 771
HapII CCGG 3 cut(s) 127, 181, 492
HgaI GACGC 3 cut(s) 327, 395, 442
HhaI GCGC 1 cut(s) 311
Hin1I GRCGYC 3 cut(s) 309, 319, 453
Hin1II CATG 4 cut(s) 85, 176, 564, 781
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HinfI GANTC 1 cut(s) 831
HpaII CCGG 3 cut(s) 127, 181, 492
HphI GGTGA 3 cut(s) 262, 448, 481
Hpy166II GTNNAC 2 cut(s) 376, 400
Hpy188III TCNNGA 3 cut(s) 43, 151, 835
Hpy8I GTNNAC 2 cut(s) 376, 400
Hpy99I CGWCG 5 cut(s) 308, 321, 350, 359, 389
HpyAV CCTTC 3 cut(s) 301, 761, 832
HpyCH4III ACNGT 4 cut(s) 397, 442, 514, 687
HpyCH4V TGCA 2 cut(s) 668, 817
HpyF3I CTNAG 2 cut(s) 54, 431
Hsp92I GRCGYC 3 cut(s) 309, 319, 453
Hsp92II CATG 4 cut(s) 85, 176, 564, 781
HspAI GCGC 1 cut(s) 309
KasI GGCGCC 1 cut(s) 308
Kzo9I GATC 3 cut(s) 16, 45, 220
LmnI GCTCC 2 cut(s) 500, 551
Lsp1109I GCAGC 9 cut(s) 652, 661, 680, 683, 801, 811, 820, 823, 829
MabI ACCWGGT 1 cut(s) 458
MaeI CTAG 3 cut(s) 92, 192, 623
MaeIII GTNAC 1 cut(s) 454
MalI GATC 3 cut(s) 18, 47, 222
MboI GATC 3 cut(s) 16, 45, 220
MboII GAAGA 7 cut(s) 99, 346, 416, 508, 511, 514, 520
MflI RGATCY 1 cut(s) 220
MhlI GDGCHC 1 cut(s) 345
MlsI TGGCCA 1 cut(s) 263
MluCI AATT 3 cut(s) 614, 784, 790
MluNI TGGCCA 1 cut(s) 263
Mly113I GGCGCC 1 cut(s) 309
MnlI CCTC 9 cut(s) 19, 63, 76, 176, 283, 293, 508, 550, 737
Mox20I TGGCCA 1 cut(s) 263
MscI TGGCCA 1 cut(s) 263
MseI TTAA 4 cut(s) 144, 447, 485, 618
MslI CAYNNNNRTG 1 cut(s) 762
Msp20I TGGCCA 1 cut(s) 263
MspA1I CMGCKG 2 cut(s) 674, 814
MspI CCGG 3 cut(s) 127, 181, 492
MspR9I CCNGG 2 cut(s) 460, 535
Mva1269I GAATGC 1 cut(s) 592
MvaI CCWGG 2 cut(s) 460, 535
MvnI CGCG 3 cut(s) 114, 332, 413
NarI GGCGCC 1 cut(s) 309
NdeII GATC 3 cut(s) 16, 45, 220
NlaIII CATG 4 cut(s) 85, 176, 564, 781
NlaIV GGNNCC 4 cut(s) 310, 382, 496, 553
NmeAIII GCCGAG 1 cut(s) 580
NmuCI GTSAC 1 cut(s) 454
NspI RCATGY 1 cut(s) 564
PaeI GCATGC 1 cut(s) 564
PceI AGGCCT 1 cut(s) 771
PcsI WCGNNNNNNNCGW 2 cut(s) 354, 393
PctI GAATGC 1 cut(s) 592
PfeI GAWTC 1 cut(s) 831
PfoI TCCNGGA 1 cut(s) 533
PluTI GGCGCC 1 cut(s) 312
Psp6I CCWGG 2 cut(s) 458, 533
PspGI CCWGG 2 cut(s) 458, 533
PspN4I GGNNCC 4 cut(s) 310, 382, 496, 553
PspPI GGNCC 2 cut(s) 50, 163
PstI CTGCAG 2 cut(s) 670, 819
PstNI CAGNNNCTG 2 cut(s) 674, 814
PsuI RGATCY 1 cut(s) 220
PvuII CAGCTG 2 cut(s) 674, 814
RsaI GTAC 3 cut(s) 353, 401, 605
RsaNI GTAC 3 cut(s) 352, 400, 604
RseI CAYNNNNRTG 1 cut(s) 762
SaqAI TTAA 4 cut(s) 144, 447, 485, 618
Sau3AI GATC 3 cut(s) 16, 45, 220
Sau96I GGNCC 2 cut(s) 50, 163
ScrFI CCNGG 2 cut(s) 460, 535
SduI GDGCHC 1 cut(s) 345
SexAI ACCWGGT 1 cut(s) 458
SfcI CTRYAG 2 cut(s) 666, 815
SfoI GGCGCC 1 cut(s) 310
SinI GGWCC 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 762
SphI GCATGC 1 cut(s) 564
Sse9I AATT 3 cut(s) 614, 784, 790
SseBI AGGCCT 1 cut(s) 771
SsiI CCGC 6 cut(s) 4, 114, 234, 330, 411, 760
SspDI GGCGCC 1 cut(s) 308
SspMI CTAG 3 cut(s) 92, 192, 623
StuI AGGCCT 1 cut(s) 771
StyD4I CCNGG 2 cut(s) 458, 533
StyI CCWWGG 1 cut(s) 772
TaaI ACNGT 4 cut(s) 397, 442, 514, 687
TaqI TCGA 1 cut(s) 834
TaqII GACCGA 1 cut(s) 151
TasI AATT 3 cut(s) 614, 784, 790
TatI WGTACW 1 cut(s) 603
TauI GCSGC 1 cut(s) 413
TfiI GAWTC 1 cut(s) 831
Tru1I TTAA 4 cut(s) 144, 447, 485, 618
Tru9I TTAA 4 cut(s) 144, 447, 485, 618
TscAI CASTG 2 cut(s) 690, 832
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 9 cut(s) 665, 668, 671, 674, 799, 808, 811, 814, 817
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 3 cut(s) 217, 283, 780
TspGWI ACGGA 3 cut(s) 238, 417, 638
TspRI CASTG 2 cut(s) 690, 832
VpaK11BI GGWCC 1 cut(s) 163
XapI RAATTY 1 cut(s) 614
XceI RCATGY 1 cut(s) 564
XspI CTAG 3 cut(s) 92, 192, 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.